AT5G08170

Porphyromonas-type peptidyl-arginine deiminase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003393
Physical Location & Seq
Reverse (-)
2628300 .. 2631295
2996 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G08170.1

Sequence Viewer

Length: 1152 bp
ATGGAGGAGTCACGAGAATCGCCGGCGGAACACGGCTACTACATGCCGGCGGAATGGGATTCTCATGCTCAAACTTGGATCGGTTGGCCTGAACGGCAAGATAACTGGCGGCACAACGCTTTACCCGCACAACGAGTGTTTGCAGATGTTGCAAAGGCCATCTCAAAGTTCGAGCCTGTCACTGTCTGTGCTAGCCCTGCTCAGTGGGAAAATGCAAGGAAACAGCTTCCAGAGGATATAAGAGTTGTTGAGATGAGCATGAATGATTCTTGGTTCCGCGACTCTGGACCAACTTTCATTGTACGGAAAAGACCAGTAAAGCTCAGTTCTCTTAACCGAAACATTGCTGGGATCGACTGGAATTTCAATGCCTGGGGAGGAGCTAATGATGGCTGTTACAATGATTGGAGTCATGACCTTTTAGTTTCACGAAAGATTCTCGCTTTGGAACGAATTCCAAGATTTCAACATTCGATGATTCTTGAAGGAGGCAGCATCCATGTCGATGGGGAAGGAACCTGCCTTGTCACAGAAGAGTGTCTCTTGAACAAAAACCGAAACCCTCATATGAGTAAAGAGCAAATAGAGGAAGAACTTAAGAAGTACCTCGGAGTACAATCATTTATCTGGCTTCCTCGTGGTCTTTACGGGGATGAGGACACAAATGGTCACATTGATAACATGTGCTGCTTCGCTAGACCGGGAGTTGTGTTATTGTCTTGGACAGACGATGAAACCGATCCTCAATACGAAAGGTCTGTGGAAGCTCTTTCGGTTTTGTCGAATTCGATTGATGCTCGTGGAAGGAAGATTCAAGTCATTAAACTTTATATCCCGGAACCCCTTTATATGACTGAAGAAGAATCGTCTGGAATCACTCAGGATGGTGAAGCTATACCAAGACTTGCAGGGACAAGACTCGCAGCATCGTATGTGAATTTCTACATCGCCAATGGAGGAATAATCGCTCCACAATTCGGTGATCCAATACGTGATAAAGAAGCGATTCGTGTCCTCTCGGATACATTTCCTCATCACTCGGTTGTGGGAATCGAGAATGCAAGAGAGATCGTTCTTGCTGGAGGAAACATTCATTGTATAACGCAGCAGCAGCCGGCGGAGCCTACTTCCGTCGCCGAAAATGGCCACTGA

Protein Analysis

383

Amino Acids

43.15

Weight (kDa)

5.13

Isoelectric Point (pI)

50.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAD_porph PF04371 15 - 372 6.3e-126 Porphyromonas-type peptidyl-arginine deiminase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 527
AccII CGCG 1 cut(s) 279
AciI CCGC 6 cut(s) 26, 50, 109, 126, 277, 1118
AclWI GGATC 4 cut(s) 86, 359, 734, 977
AcoI YGGCCR 1 cut(s) 1144
AcsI RAATTY 4 cut(s) 361, 453, 784, 937
AcuI CTGAAG 1 cut(s) 876
AfaI GTAC 3 cut(s) 303, 605, 615
AfiI CCNNNNNNNGG 1 cut(s) 977
AflII CTTAAG 1 cut(s) 596
AflIII ACRYGT 1 cut(s) 681
AgsI TTSAA 5 cut(s) 367, 467, 485, 547, 815
AjnI CCWGG 1 cut(s) 371
AluBI AGCT 5 cut(s) 226, 322, 383, 767, 893
AluI AGCT 5 cut(s) 226, 322, 383, 767, 893
Alw26I GTCTC 1 cut(s) 545
AlwI GGATC 4 cut(s) 86, 359, 734, 977
AoxI GGCC 3 cut(s) 86, 156, 1144
ApeKI GCWGC 6 cut(s) 492, 687, 923, 1105, 1108, 1111
ApoI RAATTY 4 cut(s) 361, 453, 784, 937
Asp700I GAANNNNTTC 2 cut(s) 453, 1005
AspS9I GGNCC 1 cut(s) 287
AsuC2I CCSGG 2 cut(s) 702, 836
AsuHPI GGTGA 2 cut(s) 899, 992
AsuNHI GCTAGC 1 cut(s) 191
AvaII GGWCC 1 cut(s) 287
BalI TGGCCA 1 cut(s) 1146
BauI CACGAG 3 cut(s) 12, 636, 798
BbvI GCAGC 6 cut(s) 504, 674, 935, 1117, 1120, 1123
BccI CCATC 4 cut(s) 167, 383, 500, 878
BceAI ACGGC 2 cut(s) 49, 110
BcgI CGANNNNNNTGC 2 cut(s) 484, 518
BciT130I CCWGG 1 cut(s) 373
BciVI GTATCC 1 cut(s) 1015
BcnI CCSGG 2 cut(s) 702, 836
BcoDI GTCTC 1 cut(s) 545
BfaI CTAG 2 cut(s) 192, 696
BfrI CTTAAG 1 cut(s) 596
BfuAI ACCTGC 1 cut(s) 527
BfuI GTATCC 1 cut(s) 1015
BglI GCCNNNNNGGC 1 cut(s) 94
BisI GCNGC 7 cut(s) 110, 493, 688, 924, 1106, 1109, 1112
BlsI GCNGC 7 cut(s) 111, 494, 689, 925, 1107, 1110, 1113
Bme1390I CCNGG 3 cut(s) 373, 702, 836
Bme18I GGWCC 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 4 cut(s) 275, 517, 840, 1122
BmrFI CCNGG 3 cut(s) 373, 702, 836
BmsI GCATC 3 cut(s) 504, 784, 935
BmtI GCTAGC 1 cut(s) 195
BpmI CTGGAG 1 cut(s) 1101
BpuMI CCSGG 2 cut(s) 702, 836
BsaAI YACGTR 1 cut(s) 992
BsaJI CCNNGG 2 cut(s) 372, 607
Bsc4I CCNNNNNNNGG 1 cut(s) 977
Bse118I RCCGGY 3 cut(s) 22, 46, 1114
Bse1I ACTGG 3 cut(s) 110, 314, 362
Bse3DI GCAATG 1 cut(s) 342
BseBI CCWGG 1 cut(s) 373
BseDI CCNNGG 2 cut(s) 372, 607
BseGI GGATG 3 cut(s) 495, 658, 889
BseLI CCNNNNNNNGG 1 cut(s) 977
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 3 cut(s) 215, 337, 893
BseNI ACTGG 3 cut(s) 110, 314, 362
BseRI GAGGAG 2 cut(s) 20, 393
BseXI GCAGC 6 cut(s) 504, 674, 935, 1117, 1120, 1123
BseYI CCCAGC 1 cut(s) 347
Bsh1236I CGCG 1 cut(s) 279
BshFI GGCC 3 cut(s) 88, 158, 1146
BsiSI CCGG 5 cut(s) 23, 47, 701, 836, 1115
BslFI GGGAC 1 cut(s) 925
BslI CCNNNNNNNGG 1 cut(s) 977
BsmAI GTCTC 1 cut(s) 545
BsmFI GGGAC 1 cut(s) 925
BsmI GAATGC 1 cut(s) 1063
BsnI GGCC 3 cut(s) 88, 158, 1146
Bsp143I GATC 5 cut(s) 78, 351, 739, 982, 1068
BspACI CCGC 6 cut(s) 26, 50, 109, 126, 277, 1118
BspANI GGCC 3 cut(s) 88, 158, 1146
BspCNI CTCAG 3 cut(s) 214, 336, 892
BspFNI CGCG 1 cut(s) 279
BspHI TCATGA 1 cut(s) 412
BspLI GGNNCC 4 cut(s) 275, 517, 840, 1122
BspMI ACCTGC 1 cut(s) 527
BspOI GCTAGC 1 cut(s) 195
BspPI GGATC 4 cut(s) 86, 359, 734, 977
BspTI CTTAAG 1 cut(s) 596
BsrDI GCAATG 1 cut(s) 342
BsrFI RCCGGY 3 cut(s) 22, 46, 1114
BsrI ACTGG 3 cut(s) 110, 314, 362
BssAI RCCGGY 3 cut(s) 22, 46, 1114
BssECI CCNNGG 2 cut(s) 372, 607
BssMI GATC 5 cut(s) 78, 351, 739, 982, 1068
BssSI CACGAG 3 cut(s) 12, 636, 798
Bst2BI CACGAG 3 cut(s) 12, 636, 798
Bst2UI CCWGG 1 cut(s) 373
Bst4CI ACNGT 1 cut(s) 184
Bst6I CTCTTC 1 cut(s) 528
BstAFI CTTAAG 1 cut(s) 596
BstAPI GCANNNNNTGC 1 cut(s) 149
BstBAI YACGTR 1 cut(s) 992
BstC8I GCNNGC 4 cut(s) 24, 48, 193, 1116
BstDEI CTNAG 3 cut(s) 201, 323, 879
BstF5I GGATG 3 cut(s) 495, 658, 889
BstFNI CGCG 1 cut(s) 279
BstKTI GATC 5 cut(s) 81, 354, 742, 985, 1071
BstMAI GTCTC 1 cut(s) 545
BstMBI GATC 5 cut(s) 78, 351, 739, 982, 1068
BstMWI GCNNNNNNNGC 6 cut(s) 94, 125, 149, 197, 1111, 1120
BstNI CCWGG 1 cut(s) 373
BstNSI RCATGY 2 cut(s) 46, 685
BstSCI CCNGG 3 cut(s) 371, 700, 834
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 6 cut(s) 504, 674, 935, 1117, 1120, 1123
BstXI CCANNNNNNTGG 1 cut(s) 506
BsuI GTATCC 1 cut(s) 1015
BsuRI GGCC 3 cut(s) 88, 158, 1146
BtgZI GCGATG 1 cut(s) 931
BtsCI GGATG 3 cut(s) 495, 658, 889
BtsIMutI CAGTG 3 cut(s) 180, 209, 1147
BveI ACCTGC 1 cut(s) 527
Cac8I GCNNGC 4 cut(s) 24, 48, 193, 1116
CciI TCATGA 1 cut(s) 412
Cfr10I RCCGGY 3 cut(s) 22, 46, 1114
Cfr13I GGNCC 1 cut(s) 287
Csp6I GTAC 3 cut(s) 302, 604, 614
CviAII CATG 6 cut(s) 43, 65, 259, 413, 500, 682
CviQI GTAC 3 cut(s) 302, 604, 614
DdeI CTNAG 3 cut(s) 201, 323, 879
DpnI GATC 5 cut(s) 80, 353, 741, 984, 1070
DpnII GATC 5 cut(s) 78, 351, 739, 982, 1068
EaeI YGGCCR 1 cut(s) 1144
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
EciI GGCGGA 3 cut(s) 41, 65, 1133
Eco47I GGWCC 1 cut(s) 287
Eco57I CTGAAG 1 cut(s) 876
EcoRI GAATTC 2 cut(s) 453, 784
EcoRII CCWGG 1 cut(s) 371
FaeI CATG 6 cut(s) 46, 68, 262, 416, 503, 685
FaqI GGGAC 1 cut(s) 925
FatI CATG 6 cut(s) 42, 64, 258, 412, 499, 681
FauI CCCGC 1 cut(s) 133
FauNDI CATATG 1 cut(s) 567
Fnu4HI GCNGC 7 cut(s) 110, 493, 688, 924, 1106, 1109, 1112
FokI GGATG 3 cut(s) 482, 665, 896
Fsp4HI GCNGC 7 cut(s) 110, 493, 688, 924, 1106, 1109, 1112
FspBI CTAG 2 cut(s) 192, 696
GluI GCNGC 7 cut(s) 110, 493, 688, 924, 1106, 1109, 1112
GsaI CCCAGC 1 cut(s) 351
GsuI CTGGAG 1 cut(s) 1101
HaeIII GGCC 3 cut(s) 88, 158, 1146
HapII CCGG 5 cut(s) 23, 47, 701, 836, 1115
Hin1II CATG 6 cut(s) 46, 68, 262, 416, 503, 685
HpaII CCGG 5 cut(s) 23, 47, 701, 836, 1115
HphI GGTGA 2 cut(s) 899, 992
Hpy188I TCNGA 2 cut(s) 611, 1021
Hpy99I CGWCG 1 cut(s) 1136
HpyAV CCTTC 3 cut(s) 479, 506, 798
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 1 cut(s) 991
HpyCH4V TGCA 5 cut(s) 143, 152, 215, 908, 1061
HpyF10VI GCNNNNNNNGC 6 cut(s) 94, 125, 149, 197, 1111, 1120
HpyF3I CTNAG 3 cut(s) 201, 323, 879
HpySE526I ACGT 1 cut(s) 991
Hsp92II CATG 6 cut(s) 46, 68, 262, 416, 503, 685
KroI GCCGGC 3 cut(s) 22, 46, 1114
KroNI GCCGGC 3 cut(s) 24, 48, 1116
Kzo9I GATC 5 cut(s) 78, 351, 739, 982, 1068
LmnI GCTCC 3 cut(s) 380, 973, 1120
Lsp1109I GCAGC 6 cut(s) 504, 674, 935, 1117, 1120, 1123
LweI GCATC 3 cut(s) 504, 784, 935
MaeI CTAG 2 cut(s) 192, 696
MaeII ACGT 1 cut(s) 991
MaeIII GTNAC 5 cut(s) 9, 178, 395, 526, 668
MalI GATC 5 cut(s) 80, 353, 741, 984, 1070
MboI GATC 5 cut(s) 78, 351, 739, 982, 1068
MboII GAAGA 5 cut(s) 545, 602, 820, 869, 872
MlsI TGGCCA 1 cut(s) 1146
MluCI AATT 5 cut(s) 361, 453, 784, 937, 974
MluNI TGGCCA 1 cut(s) 1146
MlyI GAGTC 4 cut(s) 17, 275, 418, 912
Mox20I TGGCCA 1 cut(s) 1146
MreI CGCCGGCG 1 cut(s) 22
MroNI GCCGGC 3 cut(s) 22, 46, 1114
MroXI GAANNNNTTC 2 cut(s) 453, 1005
MscI TGGCCA 1 cut(s) 1146
MseI TTAA 3 cut(s) 333, 597, 822
MslI CAYNNNNRTG 1 cut(s) 504
Msp20I TGGCCA 1 cut(s) 1146
MspCI CTTAAG 1 cut(s) 596
MspI CCGG 5 cut(s) 23, 47, 701, 836, 1115
MspR9I CCNGG 3 cut(s) 373, 702, 836
Mva1269I GAATGC 1 cut(s) 1063
MvaI CCWGG 1 cut(s) 373
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 6 cut(s) 94, 125, 149, 197, 1111, 1120
NaeI GCCGGC 3 cut(s) 24, 48, 1116
NciI CCSGG 2 cut(s) 702, 836
NdeI CATATG 1 cut(s) 567
NdeII GATC 5 cut(s) 78, 351, 739, 982, 1068
NgoMIV GCCGGC 3 cut(s) 22, 46, 1114
NheI GCTAGC 1 cut(s) 191
NlaIII CATG 6 cut(s) 46, 68, 262, 416, 503, 685
NlaIV GGNNCC 4 cut(s) 275, 517, 840, 1122
NmuCI GTSAC 4 cut(s) 9, 178, 526, 668
NspI RCATGY 2 cut(s) 46, 685
PagI TCATGA 1 cut(s) 412
PciI ACATGT 1 cut(s) 681
PcsI WCGNNNNNNNCGW 2 cut(s) 735, 779
PctI GAATGC 1 cut(s) 1063
PdiI GCCGGC 3 cut(s) 24, 48, 1116
PdmI GAANNNNTTC 2 cut(s) 453, 1005
PfoI TCCNGGA 1 cut(s) 834
PkrI GCNGC 7 cut(s) 111, 494, 689, 925, 1107, 1110, 1113
PleI GAGTC 4 cut(s) 16, 275, 417, 912
PpsI GAGTC 4 cut(s) 16, 275, 417, 912
Ppu21I YACGTR 1 cut(s) 992
PscI ACATGT 1 cut(s) 681
Psp6I CCWGG 1 cut(s) 371
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 1 cut(s) 371
PspN4I GGNNCC 4 cut(s) 275, 517, 840, 1122
PspPI GGNCC 1 cut(s) 287
RsaI GTAC 3 cut(s) 303, 605, 615
RsaNI GTAC 3 cut(s) 302, 604, 614
RseI CAYNNNNRTG 1 cut(s) 504
SaqAI TTAA 3 cut(s) 333, 597, 822
SatI GCNGC 7 cut(s) 110, 493, 688, 924, 1106, 1109, 1112
Sau3AI GATC 5 cut(s) 78, 351, 739, 982, 1068
Sau96I GGNCC 1 cut(s) 287
SchI GAGTC 4 cut(s) 17, 275, 418, 912
ScrFI CCNGG 3 cut(s) 373, 702, 836
SfaNI GCATC 3 cut(s) 504, 784, 935
SgrAI CRCCGGYG 1 cut(s) 22
SinI GGWCC 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 504
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
Sse9I AATT 5 cut(s) 361, 453, 784, 937, 974
SsiI CCGC 6 cut(s) 26, 50, 109, 126, 277, 1118
SspMI CTAG 2 cut(s) 192, 696
StyD4I CCNGG 3 cut(s) 371, 700, 834
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 1 cut(s) 994
TaqI TCGA 7 cut(s) 171, 354, 473, 504, 782, 788, 1053
TasI AATT 5 cut(s) 361, 453, 784, 937, 974
TatI WGTACW 1 cut(s) 613
TauI GCSGC 1 cut(s) 112
Tru1I TTAA 3 cut(s) 333, 597, 822
Tru9I TTAA 3 cut(s) 333, 597, 822
TscAI CASTG 2 cut(s) 187, 209
TseFI GTSAC 4 cut(s) 9, 178, 526, 668
TseI GCWGC 6 cut(s) 492, 687, 923, 1105, 1108, 1111
Tsp45I GTSAC 4 cut(s) 9, 178, 526, 668
TspDTI ATGAA 4 cut(s) 275, 286, 747, 1082
TspGWI ACGGA 2 cut(s) 319, 1120
TspRI CASTG 2 cut(s) 187, 209
Vha464I CTTAAG 1 cut(s) 596
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 4 cut(s) 361, 453, 784, 937
XceI RCATGY 2 cut(s) 46, 685
XmnI GAANNNNTTC 2 cut(s) 453, 1005
XspI CTAG 2 cut(s) 192, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.