Rh4BG338400

agmatine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003393
Physical Location & Seq
Forward (+)
50106505 .. 50108969
2465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG338400.1

Sequence Viewer

Length: 477 bp
ATGATTATGGTTAGTGGAACCAATTTAACTTTCTTTCTAAGAGTACAGACTGACAAACTGAAGATTTCATTAGGCGTTGATGATGATTGTTATCCAGACTGGAATCATGACCTCTTTGTGGCACGGAAGATTCTGGCAATTGAGAAGCTTCCAAGGTTTCCACATACCATGATTCTCGAAGGTGTAAGCATCCATGTAGATGGAGAAGGGACTTGCCTCACTACTGAGGAGTGCCTCCTAAATAAAAACAGGAACCCTAATTTGACCAAGGAGCAAATAAAGGATCAACTTAAGGCATATCTTGGAGTGACGAAGATTATTTGGTTGCCCCGTGGATTGTATGGTAAGAACTATACTTGGCTACTATCTAATGATTGGTTTACTTTCTTTCATTTGGGAATTAGGTCACTTATTTTTTACTTCAACCTAAACTGTGTACCAACATGCTTGGCAGGAGATGATGATACTAATGGCTAG

Protein Analysis

158

Amino Acids

18.15

Weight (kDa)

6.05

Isoelectric Point (pI)

25.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAD_porph PF04371 30 - 117 7.7e-31 Porphyromonas-type peptidyl-arginine deiminase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 291
AcuI CTGAAG 1 cut(s) 80
AfaI GTAC 2 cut(s) 45, 438
AfiI CCNNNNNNNGG 1 cut(s) 118
AflII CTTAAG 1 cut(s) 290
AgsI TTSAA 1 cut(s) 424
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AluBI AGCT 1 cut(s) 148
AluI AGCT 1 cut(s) 148
AlwI GGATC 1 cut(s) 291
BccI CCATC 1 cut(s) 194
BfaI CTAG 1 cut(s) 475
BfrI CTTAAG 1 cut(s) 290
BmiI GGNNCC 2 cut(s) 19, 254
BmsI GCATC 1 cut(s) 198
BsaBI GATNNNNATC 1 cut(s) 90
BsaJI CCNNGG 3 cut(s) 152, 267, 331
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 104
Bse8I GATNNNNATC 1 cut(s) 90
BseDI CCNNGG 3 cut(s) 152, 267, 331
BseGI GGATG 1 cut(s) 189
BseJI GATNNNNATC 1 cut(s) 90
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 104
BseRI GAGGAG 1 cut(s) 242
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 223
Bsp143I GATC 1 cut(s) 283
BspCNI CTCAG 1 cut(s) 217
BspHI TCATGA 1 cut(s) 106
BspLI GGNNCC 2 cut(s) 19, 254
BspPI GGATC 1 cut(s) 291
BspTI CTTAAG 1 cut(s) 290
BsrI ACTGG 1 cut(s) 104
BssECI CCNNGG 3 cut(s) 152, 267, 331
BssMI GATC 1 cut(s) 283
BssT1I CCWWGG 2 cut(s) 152, 267
Bst4CI ACNGT 1 cut(s) 434
BstAFI CTTAAG 1 cut(s) 290
BstDEI CTNAG 2 cut(s) 38, 225
BstDSI CCRYGG 1 cut(s) 331
BstF5I GGATG 1 cut(s) 189
BstKTI GATC 1 cut(s) 286
BstMBI GATC 1 cut(s) 283
BstNSI RCATGY 1 cut(s) 447
BstXI CCANNNNNNTGG 1 cut(s) 200
BtgI CCRYGG 1 cut(s) 331
BtsCI GGATG 1 cut(s) 189
CciI TCATGA 1 cut(s) 106
Csp6I GTAC 2 cut(s) 44, 437
CviAII CATG 4 cut(s) 107, 169, 194, 444
CviJI RGCY 3 cut(s) 148, 361, 474
CviKI_1 RGCY 3 cut(s) 148, 361, 474
CviQI GTAC 2 cut(s) 44, 437
DdeI CTNAG 2 cut(s) 38, 225
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
Eco130I CCWWGG 2 cut(s) 152, 267
Eco57I CTGAAG 1 cut(s) 80
EcoT14I CCWWGG 2 cut(s) 152, 267
ErhI CCWWGG 2 cut(s) 152, 267
FaeI CATG 4 cut(s) 110, 172, 197, 447
FaiI YATR 9 cut(s) 8, 108, 165, 170, 195, 298, 342, 354, 445
FaqI GGGAC 1 cut(s) 223
FatI CATG 4 cut(s) 106, 168, 193, 443
FokI GGATG 1 cut(s) 176
FspBI CTAG 1 cut(s) 475
Hin1II CATG 4 cut(s) 110, 172, 197, 447
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 3 cut(s) 103, 130, 172
Hpy166II GTNNAC 2 cut(s) 381, 437
Hpy188III TCNNGA 3 cut(s) 95, 107, 176
Hpy8I GTNNAC 2 cut(s) 381, 437
HpyAV CCTTC 2 cut(s) 173, 200
HpyCH4III ACNGT 1 cut(s) 434
HpyF3I CTNAG 2 cut(s) 38, 225
Hsp92II CATG 4 cut(s) 110, 172, 197, 447
Kzo9I GATC 1 cut(s) 283
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 5 cut(s) 85, 108, 119, 235, 438
LweI GCATC 1 cut(s) 198
MaeI CTAG 1 cut(s) 475
MaeIII GTNAC 2 cut(s) 307, 405
MalI GATC 1 cut(s) 285
MboI GATC 1 cut(s) 283
MboII GAAGA 3 cut(s) 73, 139, 325
MfeI CAATTG 1 cut(s) 138
MluCI AATT 4 cut(s) 22, 138, 259, 399
MnlI CCTC 4 cut(s) 122, 220, 227, 245
MseI TTAA 2 cut(s) 26, 291
MslI CAYNNNNRTG 1 cut(s) 198
MspCI CTTAAG 1 cut(s) 290
MunI CAATTG 1 cut(s) 138
NdeII GATC 1 cut(s) 283
NlaIII CATG 4 cut(s) 110, 172, 197, 447
NlaIV GGNNCC 2 cut(s) 19, 254
NmuCI GTSAC 2 cut(s) 307, 405
NspI RCATGY 1 cut(s) 447
PagI TCATGA 1 cut(s) 106
PfeI GAWTC 3 cut(s) 103, 130, 172
PspN4I GGNNCC 2 cut(s) 19, 254
RsaI GTAC 2 cut(s) 45, 438
RsaNI GTAC 2 cut(s) 44, 437
RseI CAYNNNNRTG 1 cut(s) 198
SaqAI TTAA 2 cut(s) 26, 291
Sau3AI GATC 1 cut(s) 283
SetI ASST 6 cut(s) 114, 150, 158, 184, 407, 429
SfaNI GCATC 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 198
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 4 cut(s) 22, 138, 259, 399
SspMI CTAG 1 cut(s) 475
StyI CCWWGG 2 cut(s) 152, 267
TaaI ACNGT 1 cut(s) 434
TaqI TCGA 1 cut(s) 177
TasI AATT 4 cut(s) 22, 138, 259, 399
TatI WGTACW 1 cut(s) 43
TfiI GAWTC 3 cut(s) 103, 130, 172
Tru1I TTAA 2 cut(s) 26, 291
Tru9I TTAA 2 cut(s) 26, 291
TseFI GTSAC 2 cut(s) 307, 405
Tsp45I GTSAC 2 cut(s) 307, 405
TspDTI ATGAA 2 cut(s) 57, 380
TspGWI ACGGA 1 cut(s) 139
Vha464I CTTAAG 1 cut(s) 290
XceI RCATGY 1 cut(s) 447
XspI CTAG 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.