AT5G11620

Mitogen-Activated Protein Kinase Kinase Kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004740
Physical Location & Seq
Reverse (-)
3738729 .. 3740164
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G11620.2

Sequence Viewer

Length: 822 bp
ATGGAATCTGTCGGATCCAATCAAATATCCCCGTACGTAAGTAACCGTGACCAACGGCATTTCTTGGCTCAACCGGTGGCAGATAGGATACTTCGAGCTCTCCGTCACCGGATCCGTCTTCTCCACCGTCCTAATGCAGGAACTTTTCACGTTCTTGGTGCAACGTGTAACGTCTACACAGTGACGCTAATGGCCACACCTACTTGCACTTGTCCCGACCGTAAGAAGCCGTGCAAACACATCTTGTTTGTCTTAATCCGAGTTCTTGGAATTCCTCTCGACGATAAGTGTCTCAGGCAACGGAGACTCCGGACATGCCTCCTCTTCCACCTCTTCTCTGCCCCGACACGGCCTGACTGCCTCGCCAGTTTCCGCCTCCAACAACGGTTTCTTCAGCTTTTCCCAGCCACCACTTCTCAACCCGGCTATACTACTAACTCATCCTCCTCCACCAGTAAAATGGAAAATGAGGTGGATGAAGAGCCGGCAACATGTCCAATATGTCTAGACGATATTATCGCAATAGAAAACGTAGACGGTGGAAATGGTGGAGGGAAGGAGAAGGAAACCGCGGTGGTGAAGTGCAGAGTATGCAAGAACAAGGTTCATGATGAATGCATGCTGGCGTGGAGAAAGAGTCGTGGACGGCGGCCGGCCATCTGTGTGGTATGCCGTGCACGGTGGCCAGCAAATAGATCGAGTAAGAATCCTAATGTTGGTGATAATAACGAGAACTGCCACGGTAATTGTTATTTGAATTTAGCTCCTTACGTTGATGAGGAAGTGGAGGATGGGGTTGGCACGAGCCAACGGCCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000209 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004706 GO:0004709 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006508 GO:0006511 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007254 GO:0007256 GO:0007346 GO:0008150 GO:0008152 GO:0008289 GO:0008432 GO:0009056 GO:0009057 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030163 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031434 GO:0032147 GO:0032231 GO:0032232 GO:0032268 GO:0032270 GO:0032446 GO:0032872 GO:0032874 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043632 GO:0043900 GO:0043902 GO:0043903 GO:0043933 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0046328 GO:0046330 GO:0046625 GO:0046777 GO:0046782 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048583 GO:0048584 GO:0050434 GO:0050789 GO:0050790 GO:0050792 GO:0050794 GO:0050896 GO:0051019 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051259 GO:0051338 GO:0051347 GO:0051403 GO:0051493 GO:0051494 GO:0051603 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0110053 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902531 GO:1902533 GO:1902680 GO:1902903 GO:1902904 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SWIM zf-RING_2

Protein Analysis

273

Amino Acids

30.61

Weight (kDa)

9.0

Isoelectric Point (pI)

52.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIM PF04434 59 - 88 4.2e-08 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 174, 534
AccII CGCG 1 cut(s) 572
AccIII TCCGGA 1 cut(s) 309
AciI CCGC 4 cut(s) 373, 570, 572, 649
AclWI GGATC 4 cut(s) 9, 22, 106, 119
AcoI YGGCCR 5 cut(s) 192, 650, 654, 683, 812
AcsI RAATTY 2 cut(s) 270, 757
AcuI CTGAAG 1 cut(s) 377
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 3 cut(s) 137, 348, 716
AflIII ACRYGT 2 cut(s) 164, 491
AgeI ACCGGT 1 cut(s) 73
AgsI TTSAA 1 cut(s) 757
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AluBI AGCT 3 cut(s) 98, 397, 764
AluI AGCT 3 cut(s) 98, 397, 764
Alw21I GWGCWC 2 cut(s) 100, 679
Alw26I GTCTC 2 cut(s) 296, 298
Alw44I GTGCAC 1 cut(s) 675
AlwI GGATC 4 cut(s) 9, 22, 106, 119
Aor13HI TCCGGA 1 cut(s) 309
AoxI GGCC 6 cut(s) 192, 350, 650, 654, 683, 812
ApaLI GTGCAC 1 cut(s) 675
ApoI RAATTY 2 cut(s) 270, 757
AsiGI ACCGGT 1 cut(s) 73
AsuC2I CCSGG 1 cut(s) 423
AsuHPI GGTGA 3 cut(s) 98, 589, 731
BaeGI GKGCMC 1 cut(s) 679
BalI TGGCCA 2 cut(s) 194, 685
BamHI GGATCC 2 cut(s) 14, 111
BanII GRGCYC 1 cut(s) 100
BauI CACGAG 1 cut(s) 802
BbsI GAAGAC 1 cut(s) 110
Bbv12I GWGCWC 2 cut(s) 100, 679
BccI CCATC 2 cut(s) 665, 785
BceAI ACGGC 5 cut(s) 71, 214, 365, 657, 662
BcgI CGANNNNNNTGC 2 cut(s) 678, 712
BciVI GTATCC 1 cut(s) 81
BcnI CCSGG 1 cut(s) 423
BcoDI GTCTC 2 cut(s) 296, 298
BfaI CTAG 1 cut(s) 506
BfuI GTATCC 1 cut(s) 81
BisI GCNGC 1 cut(s) 650
BlsI GCNGC 1 cut(s) 651
Bme1390I CCNGG 1 cut(s) 423
BmiI GGNNCC 2 cut(s) 16, 113
BmrFI CCNGG 1 cut(s) 423
BpiI GAAGAC 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 423
BsaAI YACGTR 1 cut(s) 37
BsaJI CCNNGG 2 cut(s) 570, 739
BsaWI WCCGGW 3 cut(s) 73, 108, 309
BsaXI ACNNNNNCTCC 6 cut(s) 291, 321, 428, 458, 622, 652
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 348, 716
Bse118I RCCGGY 3 cut(s) 73, 484, 652
Bse1I ACTGG 2 cut(s) 366, 453
BseAI TCCGGA 1 cut(s) 309
BseDI CCNNGG 2 cut(s) 570, 739
BseGI GGATG 3 cut(s) 440, 481, 796
BseLI CCNNNNNNNGG 3 cut(s) 137, 348, 716
BseMII CTCAG 1 cut(s) 307
BseNI ACTGG 2 cut(s) 366, 453
BseRI GAGGAG 2 cut(s) 311, 436
BseSI GKGCMC 1 cut(s) 679
BseX3I CGGCCG 1 cut(s) 650
BseYI CCCAGC 1 cut(s) 403
BsgI GTGCAG 1 cut(s) 604
Bsh1236I CGCG 1 cut(s) 572
Bsh1285I CGRYCG 2 cut(s) 220, 653
BshFI GGCC 6 cut(s) 194, 352, 652, 656, 685, 814
BshTI ACCGGT 1 cut(s) 73
BsiEI CGRYCG 2 cut(s) 220, 653
BsiHKAI GWGCWC 2 cut(s) 100, 679
BsiSI CCGG 6 cut(s) 74, 109, 310, 423, 485, 653
BsiWI CGTACG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 198
BslI CCNNNNNNNGG 3 cut(s) 137, 348, 716
BsmAI GTCTC 2 cut(s) 296, 298
BsmFI GGGAC 1 cut(s) 198
BsmI GAATGC 1 cut(s) 620
BsnI GGCC 6 cut(s) 194, 352, 652, 656, 685, 814
Bsp1286I GDGCHC 2 cut(s) 100, 679
Bsp13I TCCGGA 1 cut(s) 309
Bsp143I GATC 3 cut(s) 14, 111, 695
BspACI CCGC 4 cut(s) 373, 570, 572, 649
BspANI GGCC 6 cut(s) 194, 352, 652, 656, 685, 814
BspCNI CTCAG 1 cut(s) 306
BspEI TCCGGA 1 cut(s) 309
BspFNI CGCG 1 cut(s) 572
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 2 cut(s) 16, 113
BspPI GGATC 4 cut(s) 9, 22, 106, 119
BspQI GCTCTTC 1 cut(s) 474
BsrFI RCCGGY 3 cut(s) 73, 484, 652
BsrI ACTGG 2 cut(s) 366, 453
BssAI RCCGGY 3 cut(s) 73, 484, 652
BssECI CCNNGG 2 cut(s) 570, 739
BssMI GATC 3 cut(s) 14, 111, 695
BssSI CACGAG 1 cut(s) 802
Bst2BI CACGAG 1 cut(s) 802
Bst4CI ACNGT 8 cut(s) 47, 128, 181, 221, 387, 539, 681, 743
Bst6I CTCTTC 3 cut(s) 329, 338, 474
BstAPI GCANNNNNTGC 1 cut(s) 591
BstBAI YACGTR 1 cut(s) 37
BstC8I GCNNGC 5 cut(s) 486, 620, 624, 654, 687
BstDEI CTNAG 1 cut(s) 293
BstDSI CCRYGG 2 cut(s) 570, 739
BstENI CCTNNNNNAGG 1 cut(s) 135
BstF5I GGATG 3 cut(s) 440, 481, 796
BstFNI CGCG 1 cut(s) 572
BstKTI GATC 3 cut(s) 17, 114, 698
BstMAI GTCTC 2 cut(s) 296, 298
BstMBI GATC 3 cut(s) 14, 111, 695
BstMCI CGRYCG 2 cut(s) 220, 653
BstMWI GCNNNNNNNGC 1 cut(s) 591
BstNSI RCATGY 3 cut(s) 318, 495, 622
BstSCI CCNGG 1 cut(s) 421
BstSLI GKGCMC 1 cut(s) 679
BstSNI TACGTA 1 cut(s) 37
BstUI CGCG 1 cut(s) 572
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 2 cut(s) 14, 111
BstXI CCANNNNNNTGG 2 cut(s) 460, 664
BstYI RGATCY 2 cut(s) 14, 111
BstZI CGGCCG 1 cut(s) 650
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 6 cut(s) 194, 352, 652, 656, 685, 814
BtgI CCRYGG 2 cut(s) 570, 739
BtsCI GGATG 3 cut(s) 440, 481, 796
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 5 cut(s) 486, 620, 624, 654, 687
CciI TCATGA 1 cut(s) 607
Cfr10I RCCGGY 3 cut(s) 73, 484, 652
Cfr42I CCGCGG 1 cut(s) 573
CseI GACGC 1 cut(s) 193
Csp6I GTAC 1 cut(s) 34
CspAI ACCGGT 1 cut(s) 73
CspCI CAANNNNNGTGG 2 cut(s) 728, 763
CviAII CATG 5 cut(s) 315, 492, 608, 619, 816
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 1 cut(s) 293
DpnI GATC 3 cut(s) 16, 113, 697
DpnII GATC 3 cut(s) 14, 111, 695
EaeI YGGCCR 5 cut(s) 192, 650, 654, 683, 812
EagI CGGCCG 1 cut(s) 650
Eam1104I CTCTTC 3 cut(s) 329, 338, 474
EarI CTCTTC 3 cut(s) 329, 338, 474
EciI GGCGGA 1 cut(s) 362
Ecl136II GAGCTC 1 cut(s) 98
EclXI CGGCCG 1 cut(s) 650
Eco105I TACGTA 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 100
Eco52I CGGCCG 1 cut(s) 650
Eco53kI GAGCTC 1 cut(s) 98
Eco57I CTGAAG 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 98
EcoNI CCTNNNNNAGG 1 cut(s) 135
EcoRI GAATTC 1 cut(s) 270
EcoT22I ATGCAT 1 cut(s) 620
EcoT38I GRGCYC 1 cut(s) 100
FaeI CATG 5 cut(s) 318, 495, 611, 622, 819
FaiI YATR 9 cut(s) 316, 429, 493, 502, 592, 609, 620, 670, 817
FaqI GGGAC 1 cut(s) 198
FatI CATG 5 cut(s) 314, 491, 607, 618, 815
FblI GTMKAC 2 cut(s) 174, 534
Fnu4HI GCNGC 1 cut(s) 650
FokI GGATG 3 cut(s) 427, 488, 803
FriOI GRGCYC 1 cut(s) 100
FseI GGCCGGCC 1 cut(s) 656
Fsp4HI GCNGC 1 cut(s) 650
FspBI CTAG 1 cut(s) 506
GluI GCNGC 1 cut(s) 650
GsaI CCCAGC 1 cut(s) 407
HaeIII GGCC 6 cut(s) 194, 352, 652, 656, 685, 814
HapII CCGG 6 cut(s) 74, 109, 310, 423, 485, 653
HgaI GACGC 1 cut(s) 193
Hin1II CATG 5 cut(s) 318, 495, 611, 622, 819
HinfI GANTC 4 cut(s) 5, 306, 637, 706
HpaII CCGG 6 cut(s) 74, 109, 310, 423, 485, 653
HphI GGTGA 3 cut(s) 98, 589, 731
Hpy166II GTNNAC 4 cut(s) 175, 535, 644, 677
Hpy188I TCNGA 2 cut(s) 14, 260
Hpy188III TCNNGA 5 cut(s) 215, 278, 310, 506, 608
Hpy8I GTNNAC 4 cut(s) 175, 535, 644, 677
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 2 cut(s) 550, 556
HpyCH4III ACNGT 8 cut(s) 47, 128, 181, 221, 387, 539, 681, 743
HpyCH4IV ACGT 6 cut(s) 36, 150, 164, 171, 531, 771
HpyCH4V TGCA 8 cut(s) 137, 161, 207, 234, 585, 594, 618, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 591
HpyF3I CTNAG 1 cut(s) 293
HpySE526I ACGT 6 cut(s) 36, 150, 164, 171, 531, 771
Hsp92II CATG 5 cut(s) 318, 495, 611, 622, 819
Kpn2I TCCGGA 1 cut(s) 309
KroI GCCGGC 2 cut(s) 484, 652
KroNI GCCGGC 2 cut(s) 486, 654
KspI CCGCGG 1 cut(s) 573
Kzo9I GATC 3 cut(s) 14, 111, 695
LguI GCTCTTC 1 cut(s) 474
LmnI GCTCC 1 cut(s) 769
MaeI CTAG 1 cut(s) 506
MaeII ACGT 6 cut(s) 36, 150, 164, 171, 531, 771
MaeIII GTNAC 5 cut(s) 41, 47, 104, 167, 181
MalI GATC 3 cut(s) 16, 113, 697
MboI GATC 3 cut(s) 14, 111, 695
MboII GAAGA 5 cut(s) 110, 316, 325, 383, 491
MflI RGATCY 2 cut(s) 14, 111
MhlI GDGCHC 2 cut(s) 100, 679
MlsI TGGCCA 2 cut(s) 194, 685
MluCI AATT 3 cut(s) 270, 745, 757
MluNI TGGCCA 2 cut(s) 194, 685
MlyI GAGTC 2 cut(s) 300, 646
MmeI TCCRAC 1 cut(s) 403
Mox20I TGGCCA 2 cut(s) 194, 685
Mph1103I ATGCAT 1 cut(s) 620
MroI TCCGGA 1 cut(s) 309
MroNI GCCGGC 2 cut(s) 484, 652
MscI TGGCCA 2 cut(s) 194, 685
MseI TTAA 1 cut(s) 254
MslI CAYNNNNRTG 1 cut(s) 662
Msp20I TGGCCA 2 cut(s) 194, 685
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 6 cut(s) 74, 109, 310, 423, 485, 653
MspR9I CCNGG 1 cut(s) 423
Mva1269I GAATGC 1 cut(s) 620
MvnI CGCG 1 cut(s) 572
MwoI GCNNNNNNNGC 1 cut(s) 591
NaeI GCCGGC 2 cut(s) 486, 654
NciI CCSGG 1 cut(s) 423
NdeII GATC 3 cut(s) 14, 111, 695
NgoMIV GCCGGC 2 cut(s) 484, 652
NlaIII CATG 5 cut(s) 318, 495, 611, 622, 819
NlaIV GGNNCC 2 cut(s) 16, 113
NmuCI GTSAC 3 cut(s) 47, 104, 181
NsiI ATGCAT 1 cut(s) 620
NspI RCATGY 3 cut(s) 318, 495, 622
PaeI GCATGC 1 cut(s) 622
PagI TCATGA 1 cut(s) 607
PciI ACATGT 1 cut(s) 491
PciSI GCTCTTC 1 cut(s) 474
PcsI WCGNNNNNNNCGW 1 cut(s) 100
PctI GAATGC 1 cut(s) 620
PdiI GCCGGC 2 cut(s) 486, 654
PfeI GAWTC 2 cut(s) 5, 706
Pfl23II CGTACG 1 cut(s) 33
PinAI ACCGGT 1 cut(s) 73
PkrI GCNGC 1 cut(s) 651
PleI GAGTC 2 cut(s) 300, 645
PpsI GAGTC 2 cut(s) 300, 645
Ppu21I YACGTR 1 cut(s) 37
PscI ACATGT 1 cut(s) 491
Psp124BI GAGCTC 1 cut(s) 100
PspFI CCCAGC 1 cut(s) 403
PspLI CGTACG 1 cut(s) 33
PspN4I GGNNCC 2 cut(s) 16, 113
PsuI RGATCY 2 cut(s) 14, 111
RigI GGCCGGCC 1 cut(s) 656
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
RseI CAYNNNNRTG 1 cut(s) 662
SacI GAGCTC 1 cut(s) 100
SacII CCGCGG 1 cut(s) 573
SapI GCTCTTC 1 cut(s) 474
SaqAI TTAA 1 cut(s) 254
SatI GCNGC 1 cut(s) 650
Sau3AI GATC 3 cut(s) 14, 111, 695
SchI GAGTC 2 cut(s) 300, 646
ScrFI CCNGG 1 cut(s) 423
SduI GDGCHC 2 cut(s) 100, 679
Sfr303I CCGCGG 1 cut(s) 573
SgrBI CCGCGG 1 cut(s) 573
SmiMI CAYNNNNRTG 1 cut(s) 662
SnaBI TACGTA 1 cut(s) 37
SphI GCATGC 1 cut(s) 622
Sse9I AATT 3 cut(s) 270, 745, 757
SsiI CCGC 4 cut(s) 373, 570, 572, 649
SspMI CTAG 1 cut(s) 506
SstI GAGCTC 1 cut(s) 100
StyD4I CCNGG 1 cut(s) 421
TaaI ACNGT 8 cut(s) 47, 128, 181, 221, 387, 539, 681, 743
TaiI ACGT 6 cut(s) 39, 153, 167, 174, 534, 774
TaqI TCGA 3 cut(s) 94, 279, 698
TasI AATT 3 cut(s) 270, 745, 757
TauI GCSGC 1 cut(s) 652
TfiI GAWTC 2 cut(s) 5, 706
Tru1I TTAA 1 cut(s) 254
Tru9I TTAA 1 cut(s) 254
TscAI CASTG 1 cut(s) 186
TseFI GTSAC 3 cut(s) 47, 104, 181
Tsp45I GTSAC 3 cut(s) 47, 104, 181
TspDTI ATGAA 3 cut(s) 492, 596, 627
TspGWI ACGGA 3 cut(s) 92, 104, 316
TspRI CASTG 1 cut(s) 186
VneI GTGCAC 1 cut(s) 675
XagI CCTNNNNNAGG 1 cut(s) 135
XapI RAATTY 2 cut(s) 270, 757
XbaI TCTAGA 1 cut(s) 505
XceI RCATGY 3 cut(s) 318, 495, 622
XcmI CCANNNNNNNNNTGG 1 cut(s) 457
XmiI GTMKAC 2 cut(s) 174, 534
XspI CTAG 1 cut(s) 506
Zsp2I ATGCAT 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.