Rh6DG462200

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0004740
Physical Location & Seq
Reverse (-)
63125783 .. 63127585
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG462200.1

Sequence Viewer

Length: 666 bp
ATGCCGCGGCCTAGCCGCTACAAGCCCTTCAATGACCGCTTGGAGCGAGCCCTCAACCAGCACATTCGCCTCCTGCACCGCTCCGGTGCCACCTTCTTCATCCTCGGCGACACGGGCAATGTGTACACTGCGACCATCACCACTTACCCTAAATGCAGCTGCCCTGACCCTGTAACACCATGCAAGCATTTGCTGTTTGTGTATCTCCAAGTGTTGGGTCTTTCAGCCGATGGTCTCAGGGACGGCACGTTCTCGCAGTGGGAGGTGCAGTGCATGCTGGGGCGGGACACGCTGCCTGAATCGCTGGCCGGGGAAAGCGTGCGGCAGTGGTTTCATCAGCTGTATGATTTTCAGGGCAGGCAGAGGCAGCAGGGGTCTTCTTCGTCGTCGAGGCCAAGAGTGGTGATTGAAGAGGGTACTTGCTGCCCTGTTTGTTTGGATGAGATGGGGAAGCAAGACAAAGTGGTGGCTTGTGGGACGTGTAGAAACCCGATACATGAGGAATGCTTCTTGAAGTGGAAGAGGAGCGCGAGGAAGAAGCCAGCTCATTGTGTGATGTGCAGGGCGAGATGGGGGTCGATCCAGCAGGAGCAGGAGAAGTACTTGAACTTGGCGGCTTACGCTAGTACGAGCGAGGAGGATGAGGACGAAGACGAATATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000209 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004706 GO:0004709 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006508 GO:0006511 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007254 GO:0007256 GO:0007346 GO:0008150 GO:0008152 GO:0008289 GO:0008432 GO:0009056 GO:0009057 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030163 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031434 GO:0032147 GO:0032231 GO:0032232 GO:0032268 GO:0032270 GO:0032446 GO:0032872 GO:0032874 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043632 GO:0043900 GO:0043902 GO:0043903 GO:0043933 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0046328 GO:0046330 GO:0046625 GO:0046777 GO:0046782 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048583 GO:0048584 GO:0050434 GO:0050789 GO:0050790 GO:0050792 GO:0050794 GO:0050896 GO:0051019 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051259 GO:0051338 GO:0051347 GO:0051403 GO:0051493 GO:0051494 GO:0051603 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0110053 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902531 GO:1902533 GO:1902680 GO:1902903 GO:1902904 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SWIM zf-RING_2

Protein Analysis

221

Amino Acids

25.31

Weight (kDa)

6.6

Isoelectric Point (pI)

60.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 141 - 188 4.1e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AccB7I CCANNNNNTGG 1 cut(s) 214
AccBSI CCGCTC 1 cut(s) 81
AccII CGCG 2 cut(s) 7, 530
AciI CCGC 8 cut(s) 5, 7, 16, 37, 79, 283, 322, 614
AclWI GGATC 1 cut(s) 574
AcoI YGGCCR 1 cut(s) 306
AfaI GTAC 4 cut(s) 125, 418, 602, 628
AfiI CCNNNNNNNGG 1 cut(s) 214
AflIII ACRYGT 1 cut(s) 479
AgsI TTSAA 4 cut(s) 31, 410, 514, 607
AjiI CACGTC 1 cut(s) 480
AloI GAACNNNNNNTCC 2 cut(s) 233, 265
AluBI AGCT 3 cut(s) 159, 340, 545
AluI AGCT 3 cut(s) 159, 340, 545
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 1 cut(s) 574
AoxI GGCC 3 cut(s) 8, 306, 392
ApeKI GCWGC 5 cut(s) 156, 159, 292, 367, 423
AspLEI GCGC 1 cut(s) 530
AsuC2I CCSGG 1 cut(s) 310
AsuHPI GGTGA 2 cut(s) 130, 415
BanI GGYRCC 1 cut(s) 86
BanII GRGCYC 1 cut(s) 52
BbsI GAAGAC 2 cut(s) 369, 657
BbvI GCAGC 5 cut(s) 146, 168, 279, 379, 410
BccI CCATC 4 cut(s) 143, 224, 439, 564
BceAI ACGGC 1 cut(s) 259
BcnI CCSGG 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 2 cut(s) 12, 624
BmcAI AGTACT 1 cut(s) 602
Bme1390I CCNGG 1 cut(s) 310
BmgBI CACGTC 1 cut(s) 480
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 310
BpiI GAAGAC 2 cut(s) 369, 657
BpuMI CCSGG 1 cut(s) 310
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 3 cut(s) 5, 103, 309
BsaWI WCCGGW 1 cut(s) 83
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 124
BseDI CCNNGG 3 cut(s) 5, 103, 309
BseGI GGATG 3 cut(s) 99, 445, 646
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 1 cut(s) 250
BseRI GAGGAG 2 cut(s) 538, 650
BseXI GCAGC 5 cut(s) 146, 168, 279, 379, 410
BseYI CCCAGC 1 cut(s) 277
BsgI GTGCAG 3 cut(s) 59, 287, 580
Bsh1236I CGCG 2 cut(s) 7, 530
BshFI GGCC 3 cut(s) 10, 308, 394
BshNI GGYRCC 1 cut(s) 86
BsiSI CCGG 2 cut(s) 84, 309
BslFI GGGAC 3 cut(s) 254, 299, 490
BslI CCNNNNNNNGG 1 cut(s) 214
BsmAI GTCTC 1 cut(s) 239
BsmFI GGGAC 3 cut(s) 254, 299, 490
BsmI GAATGC 1 cut(s) 509
BsnI GGCC 3 cut(s) 10, 308, 394
Bso31I GGTCTC 1 cut(s) 239
Bsp1286I GDGCHC 1 cut(s) 52
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 1 cut(s) 579
BspACI CCGC 8 cut(s) 5, 7, 16, 37, 79, 283, 322, 614
BspANI GGCC 3 cut(s) 10, 308, 394
BspCNI CTCAG 1 cut(s) 249
BspFNI CGCG 2 cut(s) 7, 530
BspLI GGNNCC 1 cut(s) 88
BspPI GGATC 1 cut(s) 574
BspT107I GGYRCC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 239
BsrBI CCGCTC 1 cut(s) 81
BsrDI GCAATG 1 cut(s) 124
BsrGI TGTACA 1 cut(s) 123
BssECI CCNNGG 3 cut(s) 5, 103, 309
BssMI GATC 1 cut(s) 579
Bst6I CTCTTC 2 cut(s) 405, 515
BstAPI GCANNNNNTGC 1 cut(s) 274
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 7 cut(s) 48, 185, 275, 306, 320, 359, 543
BstDEI CTNAG 1 cut(s) 236
BstDSI CCRYGG 1 cut(s) 5
BstF5I GGATG 3 cut(s) 99, 445, 646
BstFNI CGCG 2 cut(s) 7, 530
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 1 cut(s) 582
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 1 cut(s) 579
BstMWI GCNNNNNNNGC 6 cut(s) 114, 274, 289, 301, 367, 620
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 308
BstUI CGCG 2 cut(s) 7, 530
BstV1I GCAGC 5 cut(s) 146, 168, 279, 379, 410
BstV2I GAAGAC 2 cut(s) 369, 657
BsuRI GGCC 3 cut(s) 10, 308, 394
BtgI CCRYGG 1 cut(s) 5
BtrI CACGTC 1 cut(s) 480
BtsCI GGATG 3 cut(s) 99, 445, 646
BtsI GCAGTG 4 cut(s) 126, 263, 275, 332
BtsIMutI CAGTG 4 cut(s) 126, 263, 275, 332
Cac8I GCNNGC 7 cut(s) 48, 185, 275, 306, 320, 359, 543
CfoI GCGC 1 cut(s) 530
Cfr42I CCGCGG 1 cut(s) 8
Csp6I GTAC 4 cut(s) 124, 417, 601, 627
CviAII CATG 3 cut(s) 180, 274, 497
CviQI GTAC 4 cut(s) 124, 417, 601, 627
DdeI CTNAG 1 cut(s) 236
DpnI GATC 1 cut(s) 581
DpnII GATC 1 cut(s) 579
EaeI YGGCCR 1 cut(s) 306
Eam1104I CTCTTC 2 cut(s) 405, 515
EarI CTCTTC 2 cut(s) 405, 515
Eco24I GRGCYC 1 cut(s) 52
Eco31I GGTCTC 1 cut(s) 239
EcoT38I GRGCYC 1 cut(s) 52
FaeI CATG 3 cut(s) 183, 277, 500
FaiI YATR 5 cut(s) 181, 275, 345, 498, 660
FaqI GGGAC 3 cut(s) 254, 299, 490
FatI CATG 3 cut(s) 179, 273, 496
FauI CCCGC 1 cut(s) 276
FokI GGATG 3 cut(s) 86, 452, 653
FriOI GRGCYC 1 cut(s) 52
FspBI CTAG 2 cut(s) 12, 624
GlaI GCGC 1 cut(s) 529
GsaI CCCAGC 1 cut(s) 281
HaeIII GGCC 3 cut(s) 10, 308, 394
HapII CCGG 2 cut(s) 84, 309
HhaI GCGC 1 cut(s) 530
Hin1II CATG 3 cut(s) 183, 277, 500
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HinfI GANTC 1 cut(s) 299
HpaII CCGG 2 cut(s) 84, 309
HphI GGTGA 2 cut(s) 130, 415
Hpy166II GTNNAC 2 cut(s) 124, 126
Hpy188III TCNNGA 1 cut(s) 511
Hpy8I GTNNAC 2 cut(s) 124, 126
Hpy99I CGWCG 2 cut(s) 388, 391
HpyAV CCTTC 2 cut(s) 37, 103
HpyCH4IV ACGT 2 cut(s) 248, 479
HpyCH4V TGCA 6 cut(s) 76, 156, 183, 268, 273, 561
HpyF10VI GCNNNNNNNGC 6 cut(s) 114, 274, 289, 301, 367, 620
HpyF3I CTNAG 1 cut(s) 236
HpySE526I ACGT 2 cut(s) 248, 479
Hsp92II CATG 3 cut(s) 183, 277, 500
HspAI GCGC 1 cut(s) 528
KspI CCGCGG 1 cut(s) 8
Kzo9I GATC 1 cut(s) 579
LmnI GCTCC 4 cut(s) 43, 86, 525, 589
Lsp1109I GCAGC 5 cut(s) 146, 168, 279, 379, 410
MaeI CTAG 2 cut(s) 12, 624
MaeII ACGT 2 cut(s) 248, 479
MaeIII GTNAC 1 cut(s) 172
MalI GATC 1 cut(s) 581
MbiI CCGCTC 1 cut(s) 81
MboI GATC 1 cut(s) 579
MboII GAAGA 7 cut(s) 88, 369, 372, 422, 532, 547, 662
MhlI GDGCHC 1 cut(s) 52
MspA1I CMGCKG 3 cut(s) 7, 159, 340
MspI CCGG 2 cut(s) 84, 309
MspR9I CCNGG 1 cut(s) 310
Mva1269I GAATGC 1 cut(s) 509
MvnI CGCG 2 cut(s) 7, 530
MwoI GCNNNNNNNGC 6 cut(s) 114, 274, 289, 301, 367, 620
NciI CCSGG 1 cut(s) 310
NdeII GATC 1 cut(s) 579
NlaIII CATG 3 cut(s) 183, 277, 500
NlaIV GGNNCC 1 cut(s) 88
NmeAIII GCCGAG 1 cut(s) 84
NspI RCATGY 1 cut(s) 277
PaeI GCATGC 1 cut(s) 277
PctI GAATGC 1 cut(s) 509
PfeI GAWTC 1 cut(s) 299
PflMI CCANNNNNTGG 1 cut(s) 214
PspFI CCCAGC 1 cut(s) 277
PspN4I GGNNCC 1 cut(s) 88
PvuII CAGCTG 2 cut(s) 159, 340
RsaI GTAC 4 cut(s) 125, 418, 602, 628
RsaNI GTAC 4 cut(s) 124, 417, 601, 627
SacII CCGCGG 1 cut(s) 8
Sau3AI GATC 1 cut(s) 579
ScaI AGTACT 1 cut(s) 602
ScrFI CCNGG 1 cut(s) 310
SduI GDGCHC 1 cut(s) 52
SetI ASST 7 cut(s) 95, 161, 251, 267, 342, 482, 547
Sfr303I CCGCGG 1 cut(s) 8
SgrBI CCGCGG 1 cut(s) 8
SphI GCATGC 1 cut(s) 277
SsiI CCGC 8 cut(s) 5, 7, 16, 37, 79, 283, 322, 614
SspMI CTAG 2 cut(s) 12, 624
StyD4I CCNGG 1 cut(s) 308
TaiI ACGT 2 cut(s) 251, 482
TaqI TCGA 2 cut(s) 389, 578
TatI WGTACW 2 cut(s) 123, 600
TauI GCSGC 5 cut(s) 7, 10, 18, 325, 617
TfiI GAWTC 1 cut(s) 299
TscAI CASTG 4 cut(s) 133, 263, 275, 332
TseI GCWGC 5 cut(s) 156, 159, 292, 367, 423
TspDTI ATGAA 2 cut(s) 88, 323
TspRI CASTG 4 cut(s) 133, 263, 275, 332
Van91I CCANNNNNTGG 1 cut(s) 214
XceI RCATGY 1 cut(s) 277
XspI CTAG 2 cut(s) 12, 624
ZrmI AGTACT 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.