MD08G1113400.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004740
Physical Location & Seq
Forward (+)
10059452 .. 10060198
747 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1113400.v1.1.491

Sequence Viewer

Length: 747 bp
ATGGACTCCGTCGCCTCCACCTCCTCCCCACCAAACCACCACCCCCGACGCCCGCGAACCCGGTTCAAACCCACGCAACCCTTCGCCGACCGCATTCTTCGAGCCCTCCACCACCAACTCCGCCTCCTCCACCGCCGAGACTCCAACTTCTTCGTGTTAGGCGCCACGGGGAACGTCTACACCGTCTCCTTATCCGCCACCCCTTCATGCACGTGCCCGGACCGTACAACCCCATGCAAGCACATTTTCTTCGTGTTCATCCGAGCGTTGGGCCTTCCCCTCGATGACGCATGTCTGAGGAGGAGGACGCTGCGGCCGTGCCAGGTCAACCGCCTCTTAGGGCTGCCTACGCTGCCGGAAGCACTGGCGGCTTTCAGCCTTCGAGAGAGGTTTCACCAGTTGTTTTTTCAGGGCTCCTCGTCGTCGTCAGCAACAAAGCCACGACATCAGGTGGAAATTGAAGAGGGAAGTAGCTGCCCGGTTTGTTTGGAGGAGATGGCGGGAAAGCAAGAGAAGGTGGTGGCTTGCGGGACGTGTAGAAACCCTATACACGAAGAGTGTTTGATGACGTGGAAGAGGAGCAGCGGGGGGAGGTCGGCTAGTTGCGTGATTTGTAGGGCAAGGTGGAAGGATATTAATAGGATTATGAAACAAGATAAGTACTTGAATTTGGCTGCTTATTATGCTACTAATCATGATCAAGATGACATTGGTAATGCTAGTGAGGGTCTATCGGGTGATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000209 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004706 GO:0004709 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006508 GO:0006511 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007254 GO:0007256 GO:0007346 GO:0008150 GO:0008152 GO:0008289 GO:0008432 GO:0009056 GO:0009057 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030163 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031434 GO:0032147 GO:0032231 GO:0032232 GO:0032268 GO:0032270 GO:0032446 GO:0032872 GO:0032874 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043632 GO:0043900 GO:0043902 GO:0043903 GO:0043933 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0046328 GO:0046330 GO:0046625 GO:0046777 GO:0046782 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048583 GO:0048584 GO:0050434 GO:0050789 GO:0050790 GO:0050792 GO:0050794 GO:0050896 GO:0051019 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051259 GO:0051338 GO:0051347 GO:0051403 GO:0051493 GO:0051494 GO:0051603 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0110053 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902531 GO:1902533 GO:1902680 GO:1902903 GO:1902904 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SWIM zf-RING_2

Protein Analysis

249

Amino Acids

27.8

Weight (kDa)

9.28

Isoelectric Point (pI)

69.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 158 - 206 1.2e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AccI GTMKAC 1 cut(s) 177
AccII CGCG 1 cut(s) 55
AcoI YGGCCR 1 cut(s) 314
AcsI RAATTY 1 cut(s) 667
AcvI CACGTG 1 cut(s) 213
AcyI GRCGYC 2 cut(s) 49, 162
AfaI GTAC 2 cut(s) 226, 662
AfiI CCNNNNNNNGG 1 cut(s) 268
AflIII ACRYGT 1 cut(s) 533
AgsI TTSAA 3 cut(s) 67, 461, 667
AjiI CACGTC 2 cut(s) 534, 570
AjnI CCWGG 1 cut(s) 321
AluBI AGCT 1 cut(s) 474
AluI AGCT 1 cut(s) 474
Alw26I GTCTC 2 cut(s) 132, 190
AoxI GGCC 2 cut(s) 271, 314
ApeKI GCWGC 6 cut(s) 310, 343, 352, 474, 582, 674
ApoI RAATTY 1 cut(s) 667
AseI ATTAAT 1 cut(s) 636
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 2 cut(s) 220, 271
AsuC2I CCSGG 3 cut(s) 61, 218, 479
AsuHPI GGTGA 1 cut(s) 386
AvaII GGWCC 1 cut(s) 220
BaeGI GKGCMC 1 cut(s) 218
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 2 cut(s) 106, 416
BbrPI CACGTG 1 cut(s) 213
BbvI GCAGC 6 cut(s) 297, 330, 339, 461, 594, 661
BccI CCATC 2 cut(s) 490, 734
BceAI ACGGC 1 cut(s) 301
BciT130I CCWGG 1 cut(s) 323
BclI TGATCA 1 cut(s) 697
BcnI CCSGG 3 cut(s) 61, 218, 479
BcoDI GTCTC 2 cut(s) 132, 190
BfaI CTAG 2 cut(s) 600, 720
BfoI RGCGCY 1 cut(s) 165
BisI GCNGC 8 cut(s) 311, 314, 344, 353, 369, 475, 583, 675
BlsI GCNGC 8 cut(s) 312, 315, 345, 354, 370, 476, 584, 676
BmcAI AGTACT 1 cut(s) 662
Bme1390I CCNGG 4 cut(s) 61, 218, 323, 479
Bme18I GGWCC 1 cut(s) 220
BmgBI CACGTC 2 cut(s) 534, 570
BmgT120I GGNCC 2 cut(s) 220, 271
BmiI GGNNCC 2 cut(s) 163, 415
BmrFI CCNGG 4 cut(s) 61, 218, 323, 479
BoxI GACNNNNGTC 1 cut(s) 291
BpuMI CCSGG 3 cut(s) 61, 218, 479
BsaAI YACGTR 1 cut(s) 213
BsaHI GRCGYC 2 cut(s) 49, 162
BsaJI CCNNGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 4 cut(s) 108, 138, 170, 200
Bsc4I CCNNNNNNNGG 1 cut(s) 268
Bse1I ACTGG 2 cut(s) 369, 397
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 268
BseMII CTCAG 1 cut(s) 287
BseNI ACTGG 2 cut(s) 369, 397
BseRI GAGGAG 7 cut(s) 13, 116, 313, 316, 406, 506, 592
BseSI GKGCMC 1 cut(s) 218
BseX3I CGGCCG 1 cut(s) 314
BseXI GCAGC 6 cut(s) 297, 330, 339, 461, 594, 661
Bsh1236I CGCG 1 cut(s) 55
Bsh1285I CGRYCG 2 cut(s) 91, 317
BshFI GGCC 2 cut(s) 273, 316
BshNI GGYRCC 1 cut(s) 161
BsiEI CGRYCG 2 cut(s) 91, 317
BsiSI CCGG 4 cut(s) 61, 218, 356, 479
BslFI GGGAC 1 cut(s) 544
BslI CCNNNNNNNGG 1 cut(s) 268
BsmAI GTCTC 2 cut(s) 132, 190
BsmBI CGTCTC 1 cut(s) 190
BsmFI GGGAC 1 cut(s) 544
BsmI GAATGC 1 cut(s) 93
BsnI GGCC 2 cut(s) 273, 316
Bsp1286I GDGCHC 3 cut(s) 106, 218, 416
Bsp143I GATC 1 cut(s) 697
BspANI GGCC 2 cut(s) 273, 316
BspCNI CTCAG 1 cut(s) 288
BspFNI CGCG 1 cut(s) 55
BspHI TCATGA 1 cut(s) 694
BspLI GGNNCC 2 cut(s) 163, 415
BspT107I GGYRCC 1 cut(s) 161
BsrI ACTGG 2 cut(s) 369, 397
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 1 cut(s) 697
BssNI GRCGYC 2 cut(s) 49, 162
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 2 cut(s) 184, 224
Bst6I CTCTTC 3 cut(s) 456, 549, 569
BstACI GRCGYC 2 cut(s) 49, 162
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 3 cut(s) 53, 239, 526
BstDEI CTNAG 2 cut(s) 296, 337
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 1 cut(s) 258
BstFNI CGCG 1 cut(s) 55
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 1 cut(s) 700
BstMAI GTCTC 2 cut(s) 132, 190
BstMBI GATC 1 cut(s) 697
BstMCI CGRYCG 2 cut(s) 91, 317
BstMWI GCNNNNNNNGC 4 cut(s) 349, 352, 368, 683
BstNI CCWGG 1 cut(s) 323
BstNSI RCATGY 1 cut(s) 294
BstPAI GACNNNNGTC 1 cut(s) 291
BstSCI CCNGG 4 cut(s) 59, 216, 321, 477
BstSLI GKGCMC 1 cut(s) 218
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 6 cut(s) 297, 330, 339, 461, 594, 661
BstZI CGGCCG 1 cut(s) 314
BsuRI GGCC 2 cut(s) 273, 316
BtgI CCRYGG 1 cut(s) 165
BtrI CACGTC 2 cut(s) 534, 570
BtsCI GGATG 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 362
Cac8I GCNNGC 3 cut(s) 53, 239, 526
CciI TCATGA 1 cut(s) 694
CfoI GCGC 1 cut(s) 164
Cfr13I GGNCC 2 cut(s) 220, 271
CpoI CGGWCCG 1 cut(s) 220
CseI GACGC 3 cut(s) 57, 296, 316
Csp6I GTAC 2 cut(s) 225, 661
CspI CGGWCCG 1 cut(s) 220
CviAII CATG 4 cut(s) 207, 234, 291, 695
CviQI GTAC 2 cut(s) 225, 661
DdeI CTNAG 2 cut(s) 296, 337
DinI GGCGCC 1 cut(s) 163
DpnI GATC 1 cut(s) 699
DpnII GATC 1 cut(s) 697
EaeI YGGCCR 1 cut(s) 314
EagI CGGCCG 1 cut(s) 314
Eam1104I CTCTTC 3 cut(s) 456, 549, 569
EarI CTCTTC 3 cut(s) 456, 549, 569
EciI GGCGGA 2 cut(s) 110, 184
EclXI CGGCCG 1 cut(s) 314
Eco24I GRGCYC 2 cut(s) 106, 416
Eco47I GGWCC 1 cut(s) 220
Eco52I CGGCCG 1 cut(s) 314
Eco72I CACGTG 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 321
EcoT38I GRGCYC 2 cut(s) 106, 416
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 4 cut(s) 210, 237, 294, 698
FaiI YATR 7 cut(s) 208, 235, 292, 548, 647, 684, 696
FaqI GGGAC 1 cut(s) 544
FatI CATG 4 cut(s) 206, 233, 290, 694
FauI CCCGC 4 cut(s) 60, 493, 521, 578
FbaI TGATCA 1 cut(s) 697
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 8 cut(s) 311, 314, 344, 353, 369, 475, 583, 675
FokI GGATG 1 cut(s) 245
FriOI GRGCYC 2 cut(s) 106, 416
Fsp4HI GCNGC 8 cut(s) 311, 314, 344, 353, 369, 475, 583, 675
FspBI CTAG 2 cut(s) 600, 720
GlaI GCGC 1 cut(s) 163
GluI GCNGC 8 cut(s) 311, 314, 344, 353, 369, 475, 583, 675
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 2 cut(s) 273, 316
HapII CCGG 4 cut(s) 61, 218, 356, 479
HgaI GACGC 3 cut(s) 57, 296, 316
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 2 cut(s) 49, 162
Hin1II CATG 4 cut(s) 210, 237, 294, 698
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 2 cut(s) 5, 140
HpaII CCGG 4 cut(s) 61, 218, 356, 479
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 2 cut(s) 178, 328
Hpy188I TCNGA 2 cut(s) 263, 297
Hpy188III TCNNGA 3 cut(s) 383, 695, 701
Hpy8I GTNNAC 2 cut(s) 178, 328
Hpy99I CGWCG 4 cut(s) 14, 51, 424, 427
HpyAV CCTTC 6 cut(s) 91, 213, 284, 389, 508, 622
HpyCH4III ACNGT 2 cut(s) 184, 224
HpyCH4IV ACGT 4 cut(s) 174, 212, 533, 569
HpyCH4V TGCA 2 cut(s) 210, 237
HpyF10VI GCNNNNNNNGC 4 cut(s) 349, 352, 368, 683
HpyF3I CTNAG 2 cut(s) 296, 337
HpySE526I ACGT 4 cut(s) 174, 212, 533, 569
Hsp92I GRCGYC 2 cut(s) 49, 162
Hsp92II CATG 4 cut(s) 210, 237, 294, 698
HspAI GCGC 1 cut(s) 162
KasI GGCGCC 1 cut(s) 161
Ksp22I TGATCA 1 cut(s) 697
Kzo9I GATC 1 cut(s) 697
LmnI GCTCC 2 cut(s) 419, 579
Lsp1109I GCAGC 6 cut(s) 297, 330, 339, 461, 594, 661
MaeI CTAG 2 cut(s) 600, 720
MaeII ACGT 4 cut(s) 174, 212, 533, 569
MalI GATC 1 cut(s) 699
MboI GATC 1 cut(s) 697
MboII GAAGA 6 cut(s) 89, 142, 241, 473, 566, 586
MhlI GDGCHC 3 cut(s) 106, 218, 416
MluCI AATT 2 cut(s) 456, 667
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 168
MseI TTAA 1 cut(s) 636
MslI CAYNNNNRTG 1 cut(s) 211
MspA1I CMGCKG 1 cut(s) 585
MspI CCGG 4 cut(s) 61, 218, 356, 479
MspR9I CCNGG 4 cut(s) 61, 218, 323, 479
Mva1269I GAATGC 1 cut(s) 93
MvaI CCWGG 1 cut(s) 323
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 4 cut(s) 349, 352, 368, 683
NarI GGCGCC 1 cut(s) 162
NciI CCSGG 3 cut(s) 61, 218, 479
NdeII GATC 1 cut(s) 697
NlaIII CATG 4 cut(s) 210, 237, 294, 698
NlaIV GGNNCC 2 cut(s) 163, 415
NmeAIII GCCGAG 1 cut(s) 161
NspI RCATGY 1 cut(s) 294
PagI TCATGA 1 cut(s) 694
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 314
PctI GAATGC 1 cut(s) 93
PflFI GACNNNGTC 1 cut(s) 8
PkrI GCNGC 8 cut(s) 312, 315, 345, 354, 370, 476, 584, 676
PleI GAGTC 1 cut(s) 134
PluTI GGCGCC 1 cut(s) 165
PmaCI CACGTG 1 cut(s) 213
PmlI CACGTG 1 cut(s) 213
PpsI GAGTC 1 cut(s) 134
Ppu21I YACGTR 1 cut(s) 213
PshAI GACNNNNGTC 1 cut(s) 291
PshBI ATTAAT 1 cut(s) 636
Psp6I CCWGG 1 cut(s) 321
PspCI CACGTG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 321
PspN4I GGNNCC 2 cut(s) 163, 415
PspPI GGNCC 2 cut(s) 220, 271
PsyI GACNNNGTC 1 cut(s) 8
RsaI GTAC 2 cut(s) 226, 662
RsaNI GTAC 2 cut(s) 225, 661
RseI CAYNNNNRTG 1 cut(s) 211
Rsr2I CGGWCCG 1 cut(s) 220
RsrII CGGWCCG 1 cut(s) 220
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 8 cut(s) 311, 314, 344, 353, 369, 475, 583, 675
Sau3AI GATC 1 cut(s) 697
Sau96I GGNCC 2 cut(s) 220, 271
ScaI AGTACT 1 cut(s) 662
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 4 cut(s) 61, 218, 323, 479
SduI GDGCHC 3 cut(s) 106, 218, 416
SfoI GGCGCC 1 cut(s) 163
SinI GGWCC 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 211
Sse9I AATT 2 cut(s) 456, 667
SspDI GGCGCC 1 cut(s) 161
SspMI CTAG 2 cut(s) 600, 720
StyD4I CCNGG 4 cut(s) 59, 216, 321, 477
TaaI ACNGT 2 cut(s) 184, 224
TaiI ACGT 4 cut(s) 177, 215, 536, 572
TaqI TCGA 3 cut(s) 100, 282, 382
TasI AATT 2 cut(s) 456, 667
TatI WGTACW 1 cut(s) 660
TauI GCSGC 2 cut(s) 316, 371
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TscAI CASTG 1 cut(s) 369
TseI GCWGC 6 cut(s) 310, 343, 352, 474, 582, 674
TspDTI ATGAA 3 cut(s) 195, 247, 662
TspRI CASTG 1 cut(s) 369
Tth111I GACNNNGTC 1 cut(s) 8
VpaK11BI GGWCC 1 cut(s) 220
VspI ATTAAT 1 cut(s) 636
XapI RAATTY 1 cut(s) 667
XceI RCATGY 1 cut(s) 294
XmiI GTMKAC 1 cut(s) 177
XspI CTAG 2 cut(s) 600, 720
ZrmI AGTACT 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.