FvH4_2g35480

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004740
Physical Location & Seq
Forward (+)
26146758 .. 26148104
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35480.t1

Sequence Viewer

Length: 675 bp
ATGCCGCGGGCCAGCCGCTACAAGCCGTTCAATGACAGGGTGCAGCGAGCTCTCAGCCAGCACATTCGCCTCCTGCACCGCTCCGGCGCCACCTTCTTCATCTTGGGCGACACCGGAAATGTGTACACTGCCACCATATCGACTATGCCAAAATGCAGCTGCCCTGATCGTGTGACACCCTGCAAGCATTTGCTGTTTGTGTATCTCCAAGTTTTGGGTTATTCGGAGGATGACAGGTTTCTCAGGGACGACTATTTCTCGCTGTGGGAGGTGGAGCGGATGTTGGCGCGGGACACGCTGCCTGGATCAATGGCTGGGGAGAGCATGCGGATGTGGTTTCATCAGCTGTATGATTTTGAGGGCAAGCAGAGGCAGCAGGGGTCGTCGTCCAAGACGAAGCCAAGAGTGGTGATTGAAGAGGGTACTTGCTGCCCTGTTTGTTTGGATGAGATGGGGAAGCAAGACAAGGTGGTGGCTTGTGGGACGTGTAGGAACCCGATTCATGAGGAATGCTTCTTGAAGTGGAAGAGGAGCGCGAGGAAGAAGCAGGCGCATTGTGTGATGTGCAGGGCGAGATGGGGTTCCATCGAGCAGGAGCAGGACAAGTACCTGAACTTGGCTGCTTATGCTTGTACTAGCGAGGAGGAGGATGACGATGATGAGGACGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000209 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004706 GO:0004709 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006508 GO:0006511 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007254 GO:0007256 GO:0007346 GO:0008150 GO:0008152 GO:0008289 GO:0008432 GO:0009056 GO:0009057 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030163 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031434 GO:0032147 GO:0032231 GO:0032232 GO:0032268 GO:0032270 GO:0032446 GO:0032872 GO:0032874 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043632 GO:0043900 GO:0043902 GO:0043903 GO:0043933 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0046328 GO:0046330 GO:0046625 GO:0046777 GO:0046782 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048583 GO:0048584 GO:0050434 GO:0050789 GO:0050790 GO:0050792 GO:0050794 GO:0050896 GO:0051019 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051259 GO:0051338 GO:0051347 GO:0051403 GO:0051493 GO:0051494 GO:0051603 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0110053 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902531 GO:1902533 GO:1902680 GO:1902903 GO:1902904 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SWIM zf-RING_2

Protein Analysis

225

Amino Acids

25.96

Weight (kDa)

6.1

Isoelectric Point (pI)

61.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 143 - 190 4.3e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AccB7I CCANNNNNTGG 1 cut(s) 214
AccBSI CCGCTC 2 cut(s) 81, 277
AccII CGCG 3 cut(s) 7, 289, 536
AciI CCGC 7 cut(s) 5, 7, 16, 79, 277, 289, 328
AclWI GGATC 1 cut(s) 313
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 4 cut(s) 125, 424, 608, 634
AfiI CCNNNNNNNGG 2 cut(s) 214, 616
AflIII ACRYGT 1 cut(s) 485
AgsI TTSAA 3 cut(s) 31, 416, 520
AjiI CACGTC 1 cut(s) 486
AjnI CCWGG 1 cut(s) 301
AluBI AGCT 3 cut(s) 50, 159, 346
AluI AGCT 3 cut(s) 50, 159, 346
Alw21I GWGCWC 1 cut(s) 52
AlwI GGATC 1 cut(s) 313
AoxI GGCC 1 cut(s) 9
ApeKI GCWGC 7 cut(s) 43, 156, 159, 298, 373, 429, 620
AspLEI GCGC 4 cut(s) 89, 289, 536, 553
AspS9I GGNCC 1 cut(s) 9
AsuHPI GGTGA 1 cut(s) 421
BanI GGYRCC 1 cut(s) 86
BanII GRGCYC 1 cut(s) 52
Bbv12I GWGCWC 1 cut(s) 52
BbvI GCAGC 7 cut(s) 55, 146, 168, 285, 385, 416, 607
BccI CCATC 3 cut(s) 445, 570, 593
BceAI ACGGC 1 cut(s) 10
BciT130I CCWGG 1 cut(s) 303
BfaI CTAG 2 cut(s) 636, 673
BfoI RGCGCY 1 cut(s) 90
BisI GCNGC 9 cut(s) 5, 16, 44, 157, 160, 299, 374, 430, 621
BlsI GCNGC 9 cut(s) 6, 17, 45, 158, 161, 300, 375, 431, 622
Bme1390I CCNGG 1 cut(s) 303
BmgBI CACGTC 1 cut(s) 486
BmgT120I GGNCC 1 cut(s) 9
BmiI GGNNCC 3 cut(s) 88, 494, 583
BmrFI CCNGG 1 cut(s) 303
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 1 cut(s) 5
BsaWI WCCGGW 1 cut(s) 113
BsaXI ACNNNNNCTCC 2 cut(s) 266, 296
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 616
BseBI CCWGG 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 5
BseGI GGATG 5 cut(s) 235, 285, 336, 451, 655
BseLI CCNNNNNNNGG 2 cut(s) 214, 616
BseMII CTCAG 2 cut(s) 67, 256
BseRI GAGGAG 3 cut(s) 544, 656, 659
BseXI GCAGC 7 cut(s) 55, 146, 168, 285, 385, 416, 607
BseYI CCCAGC 1 cut(s) 314
BsgI GTGCAG 3 cut(s) 59, 62, 586
Bsh1236I CGCG 3 cut(s) 7, 289, 536
BshFI GGCC 1 cut(s) 11
BshNI GGYRCC 1 cut(s) 86
BsiHKAI GWGCWC 1 cut(s) 52
BsiSI CCGG 2 cut(s) 84, 114
BslFI GGGAC 3 cut(s) 260, 305, 496
BslI CCNNNNNNNGG 2 cut(s) 214, 616
BsmFI GGGAC 3 cut(s) 260, 305, 496
BsmI GAATGC 1 cut(s) 515
BsnI GGCC 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 52
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 2 cut(s) 166, 305
BspACI CCGC 7 cut(s) 5, 7, 16, 79, 277, 289, 328
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 2 cut(s) 66, 255
BspFNI CGCG 3 cut(s) 7, 289, 536
BspHI TCATGA 1 cut(s) 502
BspLI GGNNCC 3 cut(s) 88, 494, 583
BspPI GGATC 1 cut(s) 313
BspT107I GGYRCC 1 cut(s) 86
BsrBI CCGCTC 2 cut(s) 81, 277
BsrGI TGTACA 1 cut(s) 123
BssECI CCNNGG 1 cut(s) 5
BssMI GATC 2 cut(s) 166, 305
BssNI GRCGYC 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 303
Bst6I CTCTTC 2 cut(s) 411, 521
BstACI GRCGYC 1 cut(s) 87
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 8 cut(s) 9, 13, 48, 59, 185, 326, 365, 549
BstDEI CTNAG 2 cut(s) 53, 242
BstDSI CCRYGG 1 cut(s) 5
BstF5I GGATG 5 cut(s) 235, 285, 336, 451, 655
BstFNI CGCG 3 cut(s) 7, 289, 536
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 4 cut(s) 89, 289, 536, 553
BstKTI GATC 2 cut(s) 169, 308
BstMBI GATC 2 cut(s) 166, 305
BstMWI GCNNNNNNNGC 3 cut(s) 295, 373, 626
BstNI CCWGG 1 cut(s) 303
BstNSI RCATGY 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 301
BstUI CGCG 3 cut(s) 7, 289, 536
BstV1I GCAGC 7 cut(s) 55, 146, 168, 285, 385, 416, 607
BsuRI GGCC 1 cut(s) 11
BtgI CCRYGG 1 cut(s) 5
BtrI CACGTC 1 cut(s) 486
BtsCI GGATG 5 cut(s) 235, 285, 336, 451, 655
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 8 cut(s) 9, 13, 48, 59, 185, 326, 365, 549
CciI TCATGA 1 cut(s) 502
CfoI GCGC 4 cut(s) 89, 289, 536, 553
Cfr13I GGNCC 1 cut(s) 9
Cfr42I CCGCGG 1 cut(s) 8
Csp6I GTAC 4 cut(s) 124, 423, 607, 633
CviAII CATG 2 cut(s) 325, 503
CviQI GTAC 4 cut(s) 124, 423, 607, 633
DdeI CTNAG 2 cut(s) 53, 242
DinI GGCGCC 1 cut(s) 88
DpnI GATC 2 cut(s) 168, 307
DpnII GATC 2 cut(s) 166, 305
Eam1104I CTCTTC 2 cut(s) 411, 521
EarI CTCTTC 2 cut(s) 411, 521
Ecl136II GAGCTC 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 52
Eco53kI GAGCTC 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 301
EcoT38I GRGCYC 1 cut(s) 52
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
FaeI CATG 2 cut(s) 328, 506
FaiI YATR 6 cut(s) 137, 146, 326, 351, 504, 627
FaqI GGGAC 3 cut(s) 260, 305, 496
FatI CATG 2 cut(s) 324, 502
FauI CCCGC 1 cut(s) 282
Fnu4HI GCNGC 9 cut(s) 5, 16, 44, 157, 160, 299, 374, 430, 621
FokI GGATG 5 cut(s) 242, 292, 343, 458, 662
FriOI GRGCYC 1 cut(s) 52
Fsp4HI GCNGC 9 cut(s) 5, 16, 44, 157, 160, 299, 374, 430, 621
FspBI CTAG 2 cut(s) 636, 673
GlaI GCGC 4 cut(s) 88, 288, 535, 552
GluI GCNGC 9 cut(s) 5, 16, 44, 157, 160, 299, 374, 430, 621
GsaI CCCAGC 1 cut(s) 318
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 1 cut(s) 11
HapII CCGG 2 cut(s) 84, 114
HhaI GCGC 4 cut(s) 89, 289, 536, 553
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 2 cut(s) 328, 506
Hin6I GCGC 4 cut(s) 87, 287, 534, 551
HinP1I GCGC 4 cut(s) 87, 287, 534, 551
HinfI GANTC 1 cut(s) 499
HpaII CCGG 2 cut(s) 84, 114
HphI GGTGA 1 cut(s) 421
Hpy166II GTNNAC 2 cut(s) 124, 126
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 2 cut(s) 503, 517
Hpy8I GTNNAC 2 cut(s) 124, 126
Hpy99I CGWCG 1 cut(s) 388
HpyAV CCTTC 1 cut(s) 103
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 5 cut(s) 43, 76, 156, 183, 567
HpyF10VI GCNNNNNNNGC 3 cut(s) 295, 373, 626
HpyF3I CTNAG 2 cut(s) 53, 242
HpySE526I ACGT 1 cut(s) 485
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 2 cut(s) 328, 506
HspAI GCGC 4 cut(s) 87, 287, 534, 551
KasI GGCGCC 1 cut(s) 86
KspI CCGCGG 1 cut(s) 8
Kzo9I GATC 2 cut(s) 166, 305
LmnI GCTCC 4 cut(s) 86, 274, 531, 595
Lsp1109I GCAGC 7 cut(s) 55, 146, 168, 285, 385, 416, 607
MaeI CTAG 2 cut(s) 636, 673
MaeII ACGT 1 cut(s) 485
MaeIII GTNAC 1 cut(s) 172
MalI GATC 2 cut(s) 168, 307
MbiI CCGCTC 2 cut(s) 81, 277
MboI GATC 2 cut(s) 166, 305
MboII GAAGA 4 cut(s) 88, 428, 538, 553
MhlI GDGCHC 1 cut(s) 52
Mly113I GGCGCC 1 cut(s) 87
MslI CAYNNNNRTG 1 cut(s) 329
MspA1I CMGCKG 3 cut(s) 7, 159, 346
MspI CCGG 2 cut(s) 84, 114
MspR9I CCNGG 1 cut(s) 303
Mva1269I GAATGC 1 cut(s) 515
MvaI CCWGG 1 cut(s) 303
MvnI CGCG 3 cut(s) 7, 289, 536
MwoI GCNNNNNNNGC 3 cut(s) 295, 373, 626
NarI GGCGCC 1 cut(s) 87
NdeII GATC 2 cut(s) 166, 305
NlaIII CATG 2 cut(s) 328, 506
NlaIV GGNNCC 3 cut(s) 88, 494, 583
NmuCI GTSAC 1 cut(s) 172
NspI RCATGY 1 cut(s) 328
PaeI GCATGC 1 cut(s) 328
PagI TCATGA 1 cut(s) 502
PcsI WCGNNNNNNNCGW 1 cut(s) 392
PctI GAATGC 1 cut(s) 515
PfeI GAWTC 1 cut(s) 499
PflMI CCANNNNNTGG 1 cut(s) 214
PkrI GCNGC 9 cut(s) 6, 17, 45, 158, 161, 300, 375, 431, 622
PluTI GGCGCC 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 301
PspFI CCCAGC 1 cut(s) 314
PspGI CCWGG 1 cut(s) 301
PspN4I GGNNCC 3 cut(s) 88, 494, 583
PspPI GGNCC 1 cut(s) 9
PvuII CAGCTG 2 cut(s) 159, 346
RsaI GTAC 4 cut(s) 125, 424, 608, 634
RsaNI GTAC 4 cut(s) 124, 423, 607, 633
RseI CAYNNNNRTG 1 cut(s) 329
SacI GAGCTC 1 cut(s) 52
SacII CCGCGG 1 cut(s) 8
SatI GCNGC 9 cut(s) 5, 16, 44, 157, 160, 299, 374, 430, 621
Sau3AI GATC 2 cut(s) 166, 305
Sau96I GGNCC 1 cut(s) 9
ScrFI CCNGG 1 cut(s) 303
SduI GDGCHC 1 cut(s) 52
SetI ASST 9 cut(s) 52, 95, 161, 239, 273, 348, 471, 488, 612
SfoI GGCGCC 1 cut(s) 88
Sfr303I CCGCGG 1 cut(s) 8
SgrBI CCGCGG 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 329
SphI GCATGC 1 cut(s) 328
SsiI CCGC 7 cut(s) 5, 7, 16, 79, 277, 289, 328
SspDI GGCGCC 1 cut(s) 86
SspMI CTAG 2 cut(s) 636, 673
SstI GAGCTC 1 cut(s) 52
StyD4I CCNGG 1 cut(s) 301
TaiI ACGT 1 cut(s) 488
TaqI TCGA 2 cut(s) 140, 588
TatI WGTACW 2 cut(s) 123, 632
TauI GCSGC 2 cut(s) 7, 18
TfiI GAWTC 1 cut(s) 499
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 7 cut(s) 43, 156, 159, 298, 373, 429, 620
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 3 cut(s) 88, 329, 491
TspRI CASTG 1 cut(s) 133
Van91I CCANNNNNTGG 1 cut(s) 214
XceI RCATGY 1 cut(s) 328
XspI CTAG 2 cut(s) 636, 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.