Rmu_sc0002971.1_g000027

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002971.1
OG0004740
Physical Location & Seq
Forward (+)
113526 .. 114077
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002971.1_g000027.1.cds

Sequence Viewer

Length: 552 bp
atgccgcggtggcgcaacccggacaaacctttcgaagaccgggtggagcgagccatccaccagcacatccgcctcctgcaccgctctggttccaggttcttcgttttaggacacaccaaaaatgtgtacactgtcacattgtccaccgagtcaacatgctcgtgccctgacgttgtctccccctgcaagcacctgctgcttgtgtatctccaagtattgggtgtttgtatcgacacccgtggtgttaggaggagtgcactccatccgagcgaggtgagctggctgttggggctgcccatgtcgcgcacatcactggctgaggatagtgtccgacagtggtttcatgagctccatcctcttggaaagaaacagcagcagcaggaggaggagaggcctttctcattgaggccaagcctaagtctgcgaattgaagatggtagctgctgccctgtttgcttggatgagataataaagagagataaagcggtgcattgtgggaaatgtaggaatccaatgcatgaggaatgcttcttgaagtgggggaggagctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000209 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004706 GO:0004709 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006508 GO:0006511 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007254 GO:0007256 GO:0007346 GO:0008150 GO:0008152 GO:0008289 GO:0008432 GO:0009056 GO:0009057 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030163 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031434 GO:0032147 GO:0032231 GO:0032232 GO:0032268 GO:0032270 GO:0032446 GO:0032872 GO:0032874 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043632 GO:0043900 GO:0043902 GO:0043903 GO:0043933 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0046328 GO:0046330 GO:0046625 GO:0046777 GO:0046782 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048583 GO:0048584 GO:0050434 GO:0050789 GO:0050790 GO:0050792 GO:0050794 GO:0050896 GO:0051019 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051259 GO:0051338 GO:0051347 GO:0051403 GO:0051493 GO:0051494 GO:0051603 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0110053 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902531 GO:1902533 GO:1902680 GO:1902903 GO:1902904 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SWIM zf-RING_2

Protein Analysis

183

Amino Acids

21.17

Weight (kDa)

8.87

Isoelectric Point (pI)

75.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 201
Acc36I ACCTGC 1 cut(s) 201
AccB7I CCANNNNNTGG 1 cut(s) 217
AccBSI CCGCTC 1 cut(s) 84
AccII CGCG 2 cut(s) 7, 304
AciI CCGC 5 cut(s) 5, 7, 70, 82, 485
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 2 cut(s) 431, 535
AjnI CCWGG 1 cut(s) 92
AluBI AGCT 4 cut(s) 279, 349, 441, 549
AluI AGCT 4 cut(s) 279, 349, 441, 549
Alw21I GWGCWC 2 cut(s) 259, 351
Alw26I GTCTC 1 cut(s) 181
Alw44I GTGCAC 1 cut(s) 255
AoxI GGCC 2 cut(s) 392, 407
ApaLI GTGCAC 1 cut(s) 255
ApeKI GCWGC 6 cut(s) 196, 292, 373, 376, 441, 444
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
AspLEI GCGC 2 cut(s) 15, 306
AsuC2I CCSGG 2 cut(s) 20, 41
AsuHPI GGTGA 1 cut(s) 286
AsuII TTCGAA 1 cut(s) 33
BaeGI GKGCMC 2 cut(s) 167, 259
BanII GRGCYC 1 cut(s) 351
BauI CACGAG 1 cut(s) 160
BbsI GAAGAC 1 cut(s) 42
Bbv12I GWGCWC 2 cut(s) 259, 351
BbvCI CCTCAGC 1 cut(s) 318
BbvI GCAGC 6 cut(s) 183, 279, 385, 388, 428, 431
BccI CCATC 4 cut(s) 62, 270, 360, 428
BciT130I CCWGG 1 cut(s) 94
BcnI CCSGG 2 cut(s) 20, 41
BcoDI GTCTC 1 cut(s) 181
BfaI CTAG 1 cut(s) 550
BfuAI ACCTGC 1 cut(s) 201
BglI GCCNNNNNGGC 1 cut(s) 10
BisI GCNGC 7 cut(s) 5, 197, 293, 374, 377, 442, 445
BlsI GCNGC 7 cut(s) 6, 198, 294, 375, 378, 443, 446
Bme1390I CCNGG 3 cut(s) 20, 41, 94
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 3 cut(s) 20, 41, 94
BpiI GAAGAC 1 cut(s) 42
Bpu10I CCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 33
BpuMI CCSGG 2 cut(s) 20, 41
BsaJI CCNNGG 2 cut(s) 5, 238
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse1I ACTGG 1 cut(s) 318
BseBI CCWGG 1 cut(s) 94
BseDI CCNNGG 2 cut(s) 5, 238
BseGI GGATG 5 cut(s) 54, 66, 262, 352, 466
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 1 cut(s) 318
BseRI GAGGAG 3 cut(s) 265, 398, 401
BseSI GKGCMC 2 cut(s) 167, 259
BseXI GCAGC 6 cut(s) 183, 279, 385, 388, 428, 431
BsgI GTGCAG 1 cut(s) 62
Bsh1236I CGCG 2 cut(s) 7, 304
BshFI GGCC 2 cut(s) 394, 409
BsiHKAI GWGCWC 2 cut(s) 259, 351
BsiSI CCGG 2 cut(s) 20, 40
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 1 cut(s) 181
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 2 cut(s) 394, 409
Bsp119I TTCGAA 1 cut(s) 33
Bsp1286I GDGCHC 3 cut(s) 167, 259, 351
Bsp1407I TGTACA 1 cut(s) 126
BspACI CCGC 5 cut(s) 5, 7, 70, 82, 485
BspANI GGCC 2 cut(s) 394, 409
BspCNI CTCAG 1 cut(s) 310
BspFNI CGCG 2 cut(s) 7, 304
BspHI TCATGA 1 cut(s) 343
BspLI GGNNCC 1 cut(s) 91
BspMI ACCTGC 1 cut(s) 201
BspT104I TTCGAA 1 cut(s) 33
BsrBI CCGCTC 1 cut(s) 84
BsrGI TGTACA 1 cut(s) 126
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 2 cut(s) 5, 238
BssSI CACGAG 1 cut(s) 160
Bst2BI CACGAG 1 cut(s) 160
Bst2UI CCWGG 1 cut(s) 94
Bst4CI ACNGT 2 cut(s) 133, 336
BstAPI GCANNNNNTGC 1 cut(s) 196
BstAUI TGTACA 1 cut(s) 126
BstBI TTCGAA 1 cut(s) 33
BstC8I GCNNGC 3 cut(s) 51, 188, 281
BstDEI CTNAG 2 cut(s) 318, 416
BstDSI CCRYGG 2 cut(s) 5, 238
BstF5I GGATG 5 cut(s) 54, 66, 262, 352, 466
BstFNI CGCG 2 cut(s) 7, 304
BstHHI GCGC 2 cut(s) 15, 306
BstMAI GTCTC 1 cut(s) 181
BstMWI GCNNNNNNNGC 6 cut(s) 10, 196, 276, 289, 301, 453
BstNI CCWGG 1 cut(s) 94
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 3 cut(s) 18, 39, 92
BstSLI GKGCMC 2 cut(s) 167, 259
BstUI CGCG 2 cut(s) 7, 304
BstV1I GCAGC 6 cut(s) 183, 279, 385, 388, 428, 431
BstV2I GAAGAC 1 cut(s) 42
BstXI CCANNNNNNTGG 1 cut(s) 359
BsuRI GGCC 2 cut(s) 394, 409
BtgI CCRYGG 2 cut(s) 5, 238
BtsCI GGATG 5 cut(s) 54, 66, 262, 352, 466
BtsIMutI CAGTG 3 cut(s) 129, 311, 341
BveI ACCTGC 1 cut(s) 201
Cac8I GCNNGC 3 cut(s) 51, 188, 281
CciI TCATGA 1 cut(s) 343
CfoI GCGC 2 cut(s) 15, 306
Cfr42I CCGCGG 1 cut(s) 8
Csp6I GTAC 1 cut(s) 127
CviAII CATG 4 cut(s) 156, 298, 344, 518
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 2 cut(s) 318, 416
EciI GGCGGA 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 349
Eco147I AGGCCT 1 cut(s) 394
Eco24I GRGCYC 1 cut(s) 351
Eco53kI GAGCTC 1 cut(s) 349
EcoICRI GAGCTC 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 92
EcoT22I ATGCAT 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 351
FaeI CATG 4 cut(s) 159, 301, 347, 521
FaiI YATR 4 cut(s) 157, 299, 345, 519
FatI CATG 4 cut(s) 155, 297, 343, 517
Fnu4HI GCNGC 7 cut(s) 5, 197, 293, 374, 377, 442, 445
FokI GGATG 5 cut(s) 41, 53, 249, 339, 473
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 7 cut(s) 5, 197, 293, 374, 377, 442, 445
FspBI CTAG 1 cut(s) 550
GlaI GCGC 2 cut(s) 14, 305
GluI GCNGC 7 cut(s) 5, 197, 293, 374, 377, 442, 445
HaeIII GGCC 2 cut(s) 394, 409
HapII CCGG 2 cut(s) 20, 40
HhaI GCGC 2 cut(s) 15, 306
Hin1II CATG 4 cut(s) 159, 301, 347, 521
Hin6I GCGC 2 cut(s) 13, 304
HinP1I GCGC 2 cut(s) 13, 304
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HinfI GANTC 2 cut(s) 149, 508
HpaII CCGG 2 cut(s) 20, 40
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 5 cut(s) 127, 129, 144, 153, 257
Hpy188I TCNGA 2 cut(s) 267, 332
Hpy188III TCNNGA 2 cut(s) 344, 532
Hpy8I GTNNAC 5 cut(s) 127, 129, 144, 153, 257
HpyCH4III ACNGT 2 cut(s) 133, 336
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 5 cut(s) 79, 186, 257, 490, 517
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 196, 276, 289, 301, 453
HpyF3I CTNAG 2 cut(s) 318, 416
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 4 cut(s) 159, 301, 347, 521
HspAI GCGC 2 cut(s) 13, 304
KspI CCGCGG 1 cut(s) 8
LmnI GCTCC 3 cut(s) 46, 354, 546
Lsp1109I GCAGC 6 cut(s) 183, 279, 385, 388, 428, 431
MaeI CTAG 1 cut(s) 550
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 133
MbiI CCGCTC 1 cut(s) 84
MboII GAAGA 3 cut(s) 47, 91, 443
MhlI GDGCHC 3 cut(s) 167, 259, 351
MluCI AATT 1 cut(s) 426
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 355
Mph1103I ATGCAT 1 cut(s) 519
MslI CAYNNNNRTG 1 cut(s) 160
MspA1I CMGCKG 1 cut(s) 7
MspI CCGG 2 cut(s) 20, 40
MspR9I CCNGG 3 cut(s) 20, 41, 94
Mva1269I GAATGC 1 cut(s) 530
MvaI CCWGG 1 cut(s) 94
MvnI CGCG 2 cut(s) 7, 304
MwoI GCNNNNNNNGC 6 cut(s) 10, 196, 276, 289, 301, 453
NciI CCSGG 2 cut(s) 20, 41
NlaIII CATG 4 cut(s) 159, 301, 347, 521
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 1 cut(s) 519
NspI RCATGY 1 cut(s) 159
NspV TTCGAA 1 cut(s) 33
PagI TCATGA 1 cut(s) 343
PaqCI CACCTGC 1 cut(s) 201
PceI AGGCCT 1 cut(s) 394
PctI GAATGC 1 cut(s) 530
PfeI GAWTC 1 cut(s) 508
PflFI GACNNNGTC 1 cut(s) 173
PflMI CCANNNNNTGG 1 cut(s) 217
PkrI GCNGC 7 cut(s) 6, 198, 294, 375, 378, 443, 446
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
Psp124BI GAGCTC 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 92
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 1 cut(s) 91
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
RseI CAYNNNNRTG 1 cut(s) 160
SacI GAGCTC 1 cut(s) 351
SacII CCGCGG 1 cut(s) 8
SatI GCNGC 7 cut(s) 5, 197, 293, 374, 377, 442, 445
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 3 cut(s) 20, 41, 94
SduI GDGCHC 3 cut(s) 167, 259, 351
SetI ASST 9 cut(s) 31, 98, 174, 195, 276, 281, 351, 443, 551
Sfr303I CCGCGG 1 cut(s) 8
SfuI TTCGAA 1 cut(s) 33
SgrBI CCGCGG 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 160
Sse9I AATT 1 cut(s) 426
SseBI AGGCCT 1 cut(s) 394
SsiI CCGC 5 cut(s) 5, 7, 70, 82, 485
SspMI CTAG 1 cut(s) 550
SstI GAGCTC 1 cut(s) 351
StuI AGGCCT 1 cut(s) 394
StyD4I CCNGG 3 cut(s) 18, 39, 92
TaaI ACNGT 2 cut(s) 133, 336
TaiI ACGT 1 cut(s) 174
TaqI TCGA 2 cut(s) 33, 231
TasI AATT 1 cut(s) 426
TatI WGTACW 1 cut(s) 126
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 1 cut(s) 508
TscAI CASTG 3 cut(s) 136, 318, 341
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 6 cut(s) 196, 292, 373, 376, 441, 444
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 1 cut(s) 332
TspRI CASTG 3 cut(s) 136, 318, 341
Tth111I GACNNNGTC 1 cut(s) 173
Van91I CCANNNNNTGG 1 cut(s) 217
VneI GTGCAC 1 cut(s) 255
XceI RCATGY 1 cut(s) 159
XspI CTAG 1 cut(s) 550
Zsp2I ATGCAT 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.