Prupe.1G447700_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0004740
Physical Location & Seq
Forward (+)
37834144 .. 37835382
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G447700.1

Sequence Viewer

Length: 744 bp
ATGGAGTCTGTTGCCTCCAGCTCCTCTCCACCAAACCACCACCACCCCCAACGGCCACGGACACGGTTCAAGCGCACCCAGCCCTTCGCAGACCGGATAGTCCGCGCCCTCCACCACGACCTCCGCCTCCTCCACCGCCGCGAATCCACTTTCTTCATCCTGGGCGCCACGGGGAACGTGTACACCGTGGCCTTATGCGCCACCCCTTCATGCACGTGCCCTGACCGCACAACCCCATGCAAGCACATACTCTTCGTGTTCCTCCGAGTGCTCGGCCTTCCCCTCGACGACACGTGTCTCAGGAGGAGGACGCTCCGGCCGTGCCAGGTTAGCCGCCTGTTGGGGCTGCCGACGCTGCCCGAATCACTGGCTGGTGTAAGTGTTCGACAGAGGTTTCATCAGCTGTTTTTCAACCAGGGCTCGTCAGCTTCATCGTCAAGAACAAGGCCAAAGGATGTGGAGATTGAAGAGGGCAGAAGCTGCCCTGTTTGCTTGGAGGAGATGGGAAGGGAAGAGAGAGTGGTGGCTTGCGGGACATGCAGAAACCCAATTCATGAGGAGTGTTTGATGAAGTGGAAGAGGAGCAGCGGGAGGAGGTCGGCTTATTGTGTGATTTGTAGGGCAAGGTGGAGGGATATCAATAGGACGATGGAACAAGACAAGTACTTGAATTTGACTGCTTATGCTACAAGTCATGATCAAAAAGATGATTCTGATGAGGGTCTCTGTGGTGGTGGTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000209 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004706 GO:0004709 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006508 GO:0006511 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007254 GO:0007256 GO:0007346 GO:0008150 GO:0008152 GO:0008289 GO:0008432 GO:0009056 GO:0009057 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030163 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031434 GO:0032147 GO:0032231 GO:0032232 GO:0032268 GO:0032270 GO:0032446 GO:0032872 GO:0032874 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043632 GO:0043900 GO:0043902 GO:0043903 GO:0043933 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0046328 GO:0046330 GO:0046625 GO:0046777 GO:0046782 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048583 GO:0048584 GO:0050434 GO:0050789 GO:0050790 GO:0050792 GO:0050794 GO:0050896 GO:0051019 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051259 GO:0051338 GO:0051347 GO:0051403 GO:0051493 GO:0051494 GO:0051603 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0110053 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902531 GO:1902533 GO:1902680 GO:1902903 GO:1902904 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SWIM zf-RING_2

Protein Analysis

248

Amino Acids

28.02

Weight (kDa)

9.28

Isoelectric Point (pI)

75.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
AccB1I GGYRCC 1 cut(s) 164
AccII CGCG 2 cut(s) 105, 141
AciI CCGC 8 cut(s) 103, 124, 136, 139, 226, 334, 531, 588
AcoI YGGCCR 2 cut(s) 53, 317
AcsI RAATTY 1 cut(s) 670
AcvI CACGTG 2 cut(s) 216, 294
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 2 cut(s) 182, 665
AfiI CCNNNNNNNGG 1 cut(s) 340
AflIII ACRYGT 3 cut(s) 177, 291, 293
AgsI TTSAA 4 cut(s) 70, 412, 467, 670
AjnI CCWGG 3 cut(s) 159, 324, 414
AluBI AGCT 4 cut(s) 21, 403, 428, 480
AluI AGCT 4 cut(s) 21, 403, 428, 480
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 2 cut(s) 302, 728
AlwNI CAGNNNCTG 1 cut(s) 480
AoxI GGCC 5 cut(s) 53, 189, 274, 317, 446
ApeKI GCWGC 4 cut(s) 346, 355, 480, 585
ApoI RAATTY 1 cut(s) 670
AspLEI GCGC 4 cut(s) 75, 107, 167, 200
BaeGI GKGCMC 1 cut(s) 221
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 422
BbrPI CACGTG 2 cut(s) 216, 294
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 4 cut(s) 333, 342, 467, 597
BccI CCATC 2 cut(s) 496, 643
BceAI ACGGC 2 cut(s) 68, 304
BciT130I CCWGG 3 cut(s) 161, 326, 416
BclI TGATCA 1 cut(s) 697
BcoDI GTCTC 2 cut(s) 302, 728
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 6 cut(s) 139, 334, 347, 356, 481, 586
BlsI GCNGC 6 cut(s) 140, 335, 348, 357, 482, 587
BmcAI AGTACT 1 cut(s) 665
Bme1390I CCNGG 3 cut(s) 161, 326, 416
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 3 cut(s) 161, 326, 416
BoxI GACNNNNGTC 1 cut(s) 294
BsaAI YACGTR 2 cut(s) 216, 294
BsaHI GRCGYC 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 728
BsaJI CCNNGG 5 cut(s) 56, 160, 168, 186, 415
BsaWI WCCGGW 1 cut(s) 93
Bsc4I CCNNNNNNNGG 1 cut(s) 340
Bse1I ACTGG 1 cut(s) 372
BseBI CCWGG 3 cut(s) 161, 326, 416
BseDI CCNNGG 5 cut(s) 56, 160, 168, 186, 415
BseGI GGATG 2 cut(s) 156, 460
BseLI CCNNNNNNNGG 1 cut(s) 340
BseMII CTCAG 1 cut(s) 313
BseNI ACTGG 1 cut(s) 372
BseRI GAGGAG 7 cut(s) 13, 119, 319, 512, 572, 595, 607
BseSI GKGCMC 1 cut(s) 221
BseX3I CGGCCG 1 cut(s) 317
BseXI GCAGC 4 cut(s) 333, 342, 467, 597
BseYI CCCAGC 1 cut(s) 78
Bsh1236I CGCG 2 cut(s) 105, 141
Bsh1285I CGRYCG 1 cut(s) 320
BshFI GGCC 5 cut(s) 55, 191, 276, 319, 448
BshNI GGYRCC 1 cut(s) 164
BsiEI CGRYCG 1 cut(s) 320
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 2 cut(s) 94, 316
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 1 cut(s) 340
BsmAI GTCTC 2 cut(s) 302, 728
BsmFI GGGAC 1 cut(s) 547
BsnI GGCC 5 cut(s) 55, 191, 276, 319, 448
Bso31I GGTCTC 1 cut(s) 728
Bsp1286I GDGCHC 3 cut(s) 221, 273, 422
Bsp1407I TGTACA 1 cut(s) 180
Bsp143I GATC 1 cut(s) 697
BspACI CCGC 8 cut(s) 103, 124, 136, 139, 226, 334, 531, 588
BspANI GGCC 5 cut(s) 55, 191, 276, 319, 448
BspCNI CTCAG 1 cut(s) 312
BspFNI CGCG 2 cut(s) 105, 141
BspHI TCATGA 2 cut(s) 553, 694
BspLI GGNNCC 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 164
BspTNI GGTCTC 1 cut(s) 728
BsrGI TGTACA 1 cut(s) 180
BsrI ACTGG 1 cut(s) 372
BssECI CCNNGG 5 cut(s) 56, 160, 168, 186, 415
BssMI GATC 1 cut(s) 697
BssNI GRCGYC 1 cut(s) 165
Bst2UI CCWGG 3 cut(s) 161, 326, 416
Bst4CI ACNGT 2 cut(s) 66, 187
Bst6I CTCTTC 4 cut(s) 257, 462, 507, 572
BstACI GRCGYC 1 cut(s) 165
BstAPI GCANNNNNTGC 1 cut(s) 480
BstAUI TGTACA 1 cut(s) 180
BstBAI YACGTR 2 cut(s) 216, 294
BstC8I GCNNGC 2 cut(s) 242, 529
BstDEI CTNAG 1 cut(s) 299
BstDSI CCRYGG 3 cut(s) 56, 168, 186
BstF5I GGATG 2 cut(s) 156, 460
BstFNI CGCG 2 cut(s) 105, 141
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 4 cut(s) 75, 107, 167, 200
BstKTI GATC 1 cut(s) 700
BstMAI GTCTC 2 cut(s) 302, 728
BstMBI GATC 1 cut(s) 697
BstMCI CGRYCG 1 cut(s) 320
BstMWI GCNNNNNNNGC 9 cut(s) 79, 197, 225, 330, 352, 355, 480, 489, 537
BstNI CCWGG 3 cut(s) 161, 326, 416
BstNSI RCATGY 1 cut(s) 540
BstPAI GACNNNNGTC 1 cut(s) 294
BstSCI CCNGG 3 cut(s) 159, 324, 414
BstSLI GKGCMC 1 cut(s) 221
BstUI CGCG 2 cut(s) 105, 141
BstV1I GCAGC 4 cut(s) 333, 342, 467, 597
BstZI CGGCCG 1 cut(s) 317
BsuRI GGCC 5 cut(s) 55, 191, 276, 319, 448
BtgI CCRYGG 3 cut(s) 56, 168, 186
BtsCI GGATG 2 cut(s) 156, 460
BtsIMutI CAGTG 1 cut(s) 365
Cac8I GCNNGC 2 cut(s) 242, 529
CaiI CAGNNNCTG 1 cut(s) 480
CciI TCATGA 2 cut(s) 553, 694
CfoI GCGC 4 cut(s) 75, 107, 167, 200
CseI GACGC 2 cut(s) 319, 361
Csp6I GTAC 2 cut(s) 181, 664
CspCI CAANNNNNGTGG 2 cut(s) 438, 473
CviAII CATG 5 cut(s) 210, 237, 537, 554, 695
CviQI GTAC 2 cut(s) 181, 664
DdeI CTNAG 1 cut(s) 299
DinI GGCGCC 1 cut(s) 166
DpnI GATC 1 cut(s) 699
DpnII GATC 1 cut(s) 697
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
EaeI YGGCCR 2 cut(s) 53, 317
EagI CGGCCG 1 cut(s) 317
Eam1104I CTCTTC 4 cut(s) 257, 462, 507, 572
EarI CTCTTC 4 cut(s) 257, 462, 507, 572
EciI GGCGGA 1 cut(s) 113
EclXI CGGCCG 1 cut(s) 317
Eco24I GRGCYC 1 cut(s) 422
Eco31I GGTCTC 1 cut(s) 728
Eco32I GATATC 1 cut(s) 637
Eco52I CGGCCG 1 cut(s) 317
Eco72I CACGTG 2 cut(s) 216, 294
EcoRII CCWGG 3 cut(s) 159, 324, 414
EcoRV GATATC 1 cut(s) 637
EcoT38I GRGCYC 1 cut(s) 422
EgeI GGCGCC 1 cut(s) 166
EheI GGCGCC 1 cut(s) 166
FaeI CATG 5 cut(s) 213, 240, 540, 557, 698
FaiI YATR 8 cut(s) 196, 211, 238, 248, 538, 555, 684, 696
FaqI GGGAC 1 cut(s) 547
FatI CATG 5 cut(s) 209, 236, 536, 553, 694
FauI CCCGC 2 cut(s) 524, 581
FbaI TGATCA 1 cut(s) 697
Fnu4HI GCNGC 6 cut(s) 139, 334, 347, 356, 481, 586
FokI GGATG 2 cut(s) 143, 467
FriOI GRGCYC 1 cut(s) 422
Fsp4HI GCNGC 6 cut(s) 139, 334, 347, 356, 481, 586
GlaI GCGC 4 cut(s) 74, 106, 166, 199
GluI GCNGC 6 cut(s) 139, 334, 347, 356, 481, 586
GsaI CCCAGC 1 cut(s) 82
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 5 cut(s) 55, 191, 276, 319, 448
HapII CCGG 2 cut(s) 94, 316
HgaI GACGC 2 cut(s) 319, 361
HhaI GCGC 4 cut(s) 75, 107, 167, 200
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 5 cut(s) 213, 240, 540, 557, 698
Hin6I GCGC 4 cut(s) 73, 105, 165, 198
HinP1I GCGC 4 cut(s) 73, 105, 165, 198
HinfI GANTC 4 cut(s) 5, 143, 362, 710
HpaII CCGG 2 cut(s) 94, 316
Hpy166II GTNNAC 2 cut(s) 181, 183
Hpy188I TCNGA 2 cut(s) 266, 715
Hpy188III TCNNGA 4 cut(s) 301, 438, 554, 695
Hpy8I GTNNAC 2 cut(s) 181, 183
Hpy99I CGWCG 2 cut(s) 290, 355
HpyAV CCTTC 4 cut(s) 94, 216, 287, 501
HpyCH4III ACNGT 2 cut(s) 66, 187
HpyCH4IV ACGT 3 cut(s) 177, 215, 293
HpyCH4V TGCA 3 cut(s) 213, 240, 540
HpyF10VI GCNNNNNNNGC 9 cut(s) 79, 197, 225, 330, 352, 355, 480, 489, 537
HpyF3I CTNAG 1 cut(s) 299
HpySE526I ACGT 3 cut(s) 177, 215, 293
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 5 cut(s) 213, 240, 540, 557, 698
HspAI GCGC 4 cut(s) 73, 105, 165, 198
KasI GGCGCC 1 cut(s) 164
Ksp22I TGATCA 1 cut(s) 697
Kzo9I GATC 1 cut(s) 697
LmnI GCTCC 3 cut(s) 26, 318, 582
Lsp1109I GCAGC 4 cut(s) 333, 342, 467, 597
MaeII ACGT 3 cut(s) 177, 215, 293
MalI GATC 1 cut(s) 699
MboI GATC 1 cut(s) 697
MboII GAAGA 5 cut(s) 145, 244, 479, 524, 589
MhlI GDGCHC 3 cut(s) 221, 273, 422
MluCI AATT 2 cut(s) 549, 670
Mly113I GGCGCC 1 cut(s) 165
MlyI GAGTC 1 cut(s) 14
MslI CAYNNNNRTG 1 cut(s) 214
MspA1I CMGCKG 2 cut(s) 403, 588
MspI CCGG 2 cut(s) 94, 316
MspR9I CCNGG 3 cut(s) 161, 326, 416
MvaI CCWGG 3 cut(s) 161, 326, 416
MvnI CGCG 2 cut(s) 105, 141
MwoI GCNNNNNNNGC 9 cut(s) 79, 197, 225, 330, 352, 355, 480, 489, 537
NarI GGCGCC 1 cut(s) 165
NdeII GATC 1 cut(s) 697
NlaIII CATG 5 cut(s) 213, 240, 540, 557, 698
NlaIV GGNNCC 1 cut(s) 166
NmeAIII GCCGAG 1 cut(s) 252
NspI RCATGY 1 cut(s) 540
PagI TCATGA 2 cut(s) 553, 694
PcsI WCGNNNNNNNCGW 2 cut(s) 183, 317
PfeI GAWTC 3 cut(s) 143, 362, 710
PkrI GCNGC 6 cut(s) 140, 335, 348, 357, 482, 587
PleI GAGTC 1 cut(s) 13
PluTI GGCGCC 1 cut(s) 168
PmaCI CACGTG 2 cut(s) 216, 294
PmlI CACGTG 2 cut(s) 216, 294
PpsI GAGTC 1 cut(s) 13
Ppu21I YACGTR 2 cut(s) 216, 294
PshAI GACNNNNGTC 1 cut(s) 294
Psp6I CCWGG 3 cut(s) 159, 324, 414
PspCI CACGTG 2 cut(s) 216, 294
PspFI CCCAGC 1 cut(s) 78
PspGI CCWGG 3 cut(s) 159, 324, 414
PspN4I GGNNCC 1 cut(s) 166
PstNI CAGNNNCTG 1 cut(s) 480
PvuII CAGCTG 1 cut(s) 403
RsaI GTAC 2 cut(s) 182, 665
RsaNI GTAC 2 cut(s) 181, 664
RseI CAYNNNNRTG 1 cut(s) 214
SatI GCNGC 6 cut(s) 139, 334, 347, 356, 481, 586
Sau3AI GATC 1 cut(s) 697
ScaI AGTACT 1 cut(s) 665
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 3 cut(s) 161, 326, 416
SduI GDGCHC 3 cut(s) 221, 273, 422
SfoI GGCGCC 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 214
Sse9I AATT 2 cut(s) 549, 670
SsiI CCGC 8 cut(s) 103, 124, 136, 139, 226, 334, 531, 588
SspDI GGCGCC 1 cut(s) 164
StyD4I CCNGG 3 cut(s) 159, 324, 414
TaaI ACNGT 2 cut(s) 66, 187
TaiI ACGT 3 cut(s) 180, 218, 296
TaqI TCGA 2 cut(s) 285, 385
TasI AATT 2 cut(s) 549, 670
TatI WGTACW 2 cut(s) 180, 663
TauI GCSGC 2 cut(s) 141, 336
TfiI GAWTC 3 cut(s) 143, 362, 710
TscAI CASTG 1 cut(s) 372
TseI GCWGC 4 cut(s) 346, 355, 480, 585
TspDTI ATGAA 6 cut(s) 145, 198, 386, 420, 542, 584
TspGWI ACGGA 1 cut(s) 73
TspRI CASTG 1 cut(s) 372
XapI RAATTY 1 cut(s) 670
XceI RCATGY 1 cut(s) 540
ZrmI AGTACT 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.