Rh6DG465300

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0004740
Physical Location & Seq
Forward (+)
63331179 .. 63331829
651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG465300.1

Sequence Viewer

Length: 651 bp
ATGCCACGGTGGCGCAACCTGGACAAACCTTTCGAAGACCGGGTGGAGCGAGCCATCCACCAGCACATCCGCCTCCTGCACCGCTCTGGTTCCAGGTTCTTCGTTTTAGGACACACCAAAAATGTGTACACTGTCACATTGTCCACCGAGTCAACATGCTCGTGCCCTGACGTTGTCTCCCCCTGCAAGCACCTGCTGTTTGTGTATCTCCAAGTATTGGGTGTTTGTATCGACACCCGTTGTGTTAGGAGGAGCGCACTCCATCCAAGCGAGGTGAGCTGGCTGTTGGGGTTGCCCATGTCACGCGCATCACTGGCTGAGGATAGTGTCCGACACTGGTTTCATCAGATCCATCCTCTTGGAAAGAAACAGCAGCAGCAGGAGAGGTCTTTCTCATCGAGGCCAAGCCTAATTCTGCAAATTGAAGATGGTAGCTGCTGCCCTGTTTGCTTGGATGAGATAAGAAAGAGAGATAAAGCGGTGCATTGTGGGCAATGTAGGAATCCAATGCATGAGGAATGCTTCTTGAAGTGGGGGAGGAGCGCAAGGAACAAGTCGCCTCATTGTGCAATATGCAATGCAAAATGGAGCAGTACAATCAGCCGCGAGCAGGCGCAGCACATGTACTTGAATTTAGCTGCTTACGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000209 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004706 GO:0004709 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006508 GO:0006511 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007254 GO:0007256 GO:0007346 GO:0008150 GO:0008152 GO:0008289 GO:0008432 GO:0009056 GO:0009057 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030163 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031434 GO:0032147 GO:0032231 GO:0032232 GO:0032268 GO:0032270 GO:0032446 GO:0032872 GO:0032874 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043632 GO:0043900 GO:0043902 GO:0043903 GO:0043933 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0046328 GO:0046330 GO:0046625 GO:0046777 GO:0046782 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048583 GO:0048584 GO:0050434 GO:0050789 GO:0050790 GO:0050792 GO:0050794 GO:0050896 GO:0051019 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051259 GO:0051338 GO:0051347 GO:0051403 GO:0051493 GO:0051494 GO:0051603 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0110053 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902531 GO:1902533 GO:1902680 GO:1902903 GO:1902904 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SWIM zf-RING_2

Protein Analysis

216

Amino Acids

24.95

Weight (kDa)

9.12

Isoelectric Point (pI)

75.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIM PF04434 43 - 71 7.2e-06 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 201
Acc36I ACCTGC 1 cut(s) 201
AccB7I CCANNNNNTGG 1 cut(s) 217
AccBSI CCGCTC 1 cut(s) 84
AccII CGCG 2 cut(s) 306, 606
AciI CCGC 4 cut(s) 70, 82, 479, 604
AclWI GGATC 1 cut(s) 343
AcsI RAATTY 1 cut(s) 631
AfaI GTAC 3 cut(s) 128, 595, 626
AfiI CCNNNNNNNGG 2 cut(s) 217, 610
AflIII ACRYGT 1 cut(s) 621
AgsI TTSAA 3 cut(s) 425, 529, 631
AjnI CCWGG 2 cut(s) 18, 92
AluBI AGCT 3 cut(s) 279, 435, 638
AluI AGCT 3 cut(s) 279, 435, 638
Alw26I GTCTC 1 cut(s) 181
AlwI GGATC 1 cut(s) 343
AoxI GGCC 1 cut(s) 401
ApeKI GCWGC 6 cut(s) 373, 376, 435, 438, 616, 638
ApoI RAATTY 1 cut(s) 631
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
AspLEI GCGC 5 cut(s) 15, 257, 308, 545, 616
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 1 cut(s) 286
AsuII TTCGAA 1 cut(s) 33
BaeGI GKGCMC 1 cut(s) 167
BauI CACGAG 1 cut(s) 160
BbsI GAAGAC 1 cut(s) 42
BbvCI CCTCAGC 1 cut(s) 318
BbvI GCAGC 6 cut(s) 385, 388, 422, 425, 625, 628
BccI CCATC 4 cut(s) 62, 270, 360, 422
BciT130I CCWGG 2 cut(s) 20, 94
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 1 cut(s) 181
BfaI CTAG 1 cut(s) 649
BfuAI ACCTGC 1 cut(s) 201
BglI GCCNNNNNGGC 1 cut(s) 10
BisI GCNGC 7 cut(s) 374, 377, 436, 439, 604, 617, 639
BlsI GCNGC 7 cut(s) 375, 378, 437, 440, 605, 618, 640
Bme1390I CCNGG 3 cut(s) 20, 41, 94
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 3 cut(s) 20, 41, 94
BmsI GCATC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 42
Bpu10I CCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 33
BpuMI CCSGG 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 5
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 610
Bse1I ACTGG 2 cut(s) 318, 341
Bse3DI GCAATG 2 cut(s) 500, 583
BseBI CCWGG 2 cut(s) 20, 94
BseDI CCNNGG 1 cut(s) 5
BseGI GGATG 5 cut(s) 54, 66, 262, 352, 460
BseLI CCNNNNNNNGG 2 cut(s) 217, 610
BseMI GCAATG 2 cut(s) 500, 583
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 2 cut(s) 318, 341
BseRI GAGGAG 2 cut(s) 265, 553
BseSI GKGCMC 1 cut(s) 167
BseXI GCAGC 6 cut(s) 385, 388, 422, 425, 625, 628
BsgI GTGCAG 1 cut(s) 62
Bsh1236I CGCG 2 cut(s) 306, 606
BshFI GGCC 1 cut(s) 403
BsiSI CCGG 1 cut(s) 40
BslI CCNNNNNNNGG 2 cut(s) 217, 610
BsmAI GTCTC 1 cut(s) 181
BsmI GAATGC 1 cut(s) 524
BsnI GGCC 1 cut(s) 403
Bsp119I TTCGAA 1 cut(s) 33
Bsp1286I GDGCHC 1 cut(s) 167
Bsp1407I TGTACA 1 cut(s) 126
Bsp143I GATC 1 cut(s) 348
BspACI CCGC 4 cut(s) 70, 82, 479, 604
BspANI GGCC 1 cut(s) 403
BspCNI CTCAG 1 cut(s) 310
BspFNI CGCG 2 cut(s) 306, 606
BspLI GGNNCC 1 cut(s) 91
BspMI ACCTGC 1 cut(s) 201
BspPI GGATC 1 cut(s) 343
BspT104I TTCGAA 1 cut(s) 33
BsrBI CCGCTC 1 cut(s) 84
BsrDI GCAATG 2 cut(s) 500, 583
BsrGI TGTACA 1 cut(s) 126
BsrI ACTGG 2 cut(s) 318, 341
BssECI CCNNGG 1 cut(s) 5
BssMI GATC 1 cut(s) 348
BssSI CACGAG 1 cut(s) 160
Bst2BI CACGAG 1 cut(s) 160
Bst2UI CCWGG 2 cut(s) 20, 94
Bst4CI ACNGT 2 cut(s) 9, 133
BstAUI TGTACA 1 cut(s) 126
BstBI TTCGAA 1 cut(s) 33
BstC8I GCNNGC 5 cut(s) 51, 188, 281, 608, 612
BstDEI CTNAG 1 cut(s) 318
BstDSI CCRYGG 1 cut(s) 5
BstF5I GGATG 5 cut(s) 54, 66, 262, 352, 460
BstFNI CGCG 2 cut(s) 306, 606
BstHHI GCGC 5 cut(s) 15, 257, 308, 545, 616
BstKTI GATC 1 cut(s) 351
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 1 cut(s) 348
BstMWI GCNNNNNNNGC 6 cut(s) 10, 276, 314, 447, 490, 616
BstNI CCWGG 2 cut(s) 20, 94
BstNSI RCATGY 2 cut(s) 159, 625
BstSCI CCNGG 3 cut(s) 18, 39, 92
BstSLI GKGCMC 1 cut(s) 167
BstUI CGCG 2 cut(s) 306, 606
BstV1I GCAGC 6 cut(s) 385, 388, 422, 425, 625, 628
BstV2I GAAGAC 1 cut(s) 42
BstX2I RGATCY 1 cut(s) 348
BstXI CCANNNNNNTGG 1 cut(s) 359
BstYI RGATCY 1 cut(s) 348
BsuRI GGCC 1 cut(s) 403
BtgI CCRYGG 1 cut(s) 5
BtsCI GGATG 5 cut(s) 54, 66, 262, 352, 460
BtsIMutI CAGTG 3 cut(s) 129, 311, 334
BveI ACCTGC 1 cut(s) 201
Cac8I GCNNGC 5 cut(s) 51, 188, 281, 608, 612
CfoI GCGC 5 cut(s) 15, 257, 308, 545, 616
Csp6I GTAC 3 cut(s) 127, 594, 625
CviAII CATG 4 cut(s) 156, 298, 512, 622
CviJI RGCY 9 cut(s) 53, 279, 283, 317, 403, 408, 435, 603, 638
CviKI_1 RGCY 9 cut(s) 53, 279, 283, 317, 403, 408, 435, 603, 638
CviQI GTAC 3 cut(s) 127, 594, 625
DdeI CTNAG 1 cut(s) 318
DpnI GATC 1 cut(s) 350
DpnII GATC 1 cut(s) 348
EciI GGCGGA 1 cut(s) 59
EcoRII CCWGG 2 cut(s) 18, 92
EcoT22I ATGCAT 1 cut(s) 513
FaeI CATG 4 cut(s) 159, 301, 515, 625
FaiI YATR 5 cut(s) 157, 299, 513, 574, 623
FatI CATG 4 cut(s) 155, 297, 511, 621
Fnu4HI GCNGC 7 cut(s) 374, 377, 436, 439, 604, 617, 639
FokI GGATG 5 cut(s) 41, 53, 249, 339, 467
Fsp4HI GCNGC 7 cut(s) 374, 377, 436, 439, 604, 617, 639
FspBI CTAG 1 cut(s) 649
GlaI GCGC 5 cut(s) 14, 256, 307, 544, 615
GluI GCNGC 7 cut(s) 374, 377, 436, 439, 604, 617, 639
HaeIII GGCC 1 cut(s) 403
HapII CCGG 1 cut(s) 40
HhaI GCGC 5 cut(s) 15, 257, 308, 545, 616
Hin1II CATG 4 cut(s) 159, 301, 515, 625
Hin6I GCGC 5 cut(s) 13, 255, 306, 543, 614
HinP1I GCGC 5 cut(s) 13, 255, 306, 543, 614
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HinfI GANTC 2 cut(s) 149, 502
HpaII CCGG 1 cut(s) 40
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 4 cut(s) 127, 129, 144, 153
Hpy188I TCNGA 2 cut(s) 332, 348
Hpy188III TCNNGA 1 cut(s) 526
Hpy8I GTNNAC 4 cut(s) 127, 129, 144, 153
HpyCH4III ACNGT 2 cut(s) 9, 133
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 8 cut(s) 79, 186, 418, 484, 511, 569, 576, 581
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 276, 314, 447, 490, 616
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 4 cut(s) 159, 301, 515, 625
HspAI GCGC 5 cut(s) 13, 255, 306, 543, 614
Kzo9I GATC 1 cut(s) 348
LmnI GCTCC 4 cut(s) 46, 252, 540, 588
Lsp1109I GCAGC 6 cut(s) 385, 388, 422, 425, 625, 628
LweI GCATC 1 cut(s) 317
MaeI CTAG 1 cut(s) 649
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 133, 300
MalI GATC 1 cut(s) 350
MbiI CCGCTC 1 cut(s) 84
MboI GATC 1 cut(s) 348
MboII GAAGA 3 cut(s) 47, 91, 437
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 1 cut(s) 167
MluCI AATT 3 cut(s) 411, 420, 631
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 355
Mph1103I ATGCAT 1 cut(s) 513
MslI CAYNNNNRTG 1 cut(s) 160
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 3 cut(s) 20, 41, 94
Mva1269I GAATGC 1 cut(s) 524
MvaI CCWGG 2 cut(s) 20, 94
MvnI CGCG 2 cut(s) 306, 606
MwoI GCNNNNNNNGC 6 cut(s) 10, 276, 314, 447, 490, 616
NciI CCSGG 1 cut(s) 41
NdeII GATC 1 cut(s) 348
NlaIII CATG 4 cut(s) 159, 301, 515, 625
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 2 cut(s) 133, 300
NsiI ATGCAT 1 cut(s) 513
NspI RCATGY 2 cut(s) 159, 625
NspV TTCGAA 1 cut(s) 33
PaqCI CACCTGC 1 cut(s) 201
PciI ACATGT 1 cut(s) 621
PctI GAATGC 1 cut(s) 524
PfeI GAWTC 1 cut(s) 502
PflFI GACNNNGTC 1 cut(s) 173
PflMI CCANNNNNTGG 1 cut(s) 217
PkrI GCNGC 7 cut(s) 375, 378, 437, 440, 605, 618, 640
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PscI ACATGT 1 cut(s) 621
Psp6I CCWGG 2 cut(s) 18, 92
PspGI CCWGG 2 cut(s) 18, 92
PspN4I GGNNCC 1 cut(s) 91
PsuI RGATCY 1 cut(s) 348
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 3 cut(s) 128, 595, 626
RsaNI GTAC 3 cut(s) 127, 594, 625
RseI CAYNNNNRTG 1 cut(s) 160
SatI GCNGC 7 cut(s) 374, 377, 436, 439, 604, 617, 639
Sau3AI GATC 1 cut(s) 348
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 3 cut(s) 20, 41, 94
SduI GDGCHC 1 cut(s) 167
SfaNI GCATC 1 cut(s) 317
SfuI TTCGAA 1 cut(s) 33
SmiMI CAYNNNNRTG 1 cut(s) 160
Sse9I AATT 3 cut(s) 411, 420, 631
SsiI CCGC 4 cut(s) 70, 82, 479, 604
SspMI CTAG 1 cut(s) 649
StyD4I CCNGG 3 cut(s) 18, 39, 92
TaaI ACNGT 2 cut(s) 9, 133
TaiI ACGT 1 cut(s) 174
TaqI TCGA 3 cut(s) 33, 231, 398
TasI AATT 3 cut(s) 411, 420, 631
TatI WGTACW 3 cut(s) 126, 593, 624
TauI GCSGC 1 cut(s) 606
TfiI GAWTC 1 cut(s) 502
TscAI CASTG 3 cut(s) 136, 318, 341
TseFI GTSAC 2 cut(s) 133, 300
TseI GCWGC 6 cut(s) 373, 376, 435, 438, 616, 638
Tsp45I GTSAC 2 cut(s) 133, 300
TspDTI ATGAA 1 cut(s) 332
TspRI CASTG 3 cut(s) 136, 318, 341
Tth111I GACNNNGTC 1 cut(s) 173
Van91I CCANNNNNTGG 1 cut(s) 217
XapI RAATTY 1 cut(s) 631
XceI RCATGY 2 cut(s) 159, 625
XspI CTAG 1 cut(s) 649
Zsp2I ATGCAT 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.