Rmu_sc0003473.1_g000010

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003473.1
OG0004740
Physical Location & Seq
Forward (+)
41105 .. 41721
617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003473.1_g000010.1.cds

Sequence Viewer

Length: 510 bp
atgcaccgctccagtgccacattcttcatcttgggagtcactggcaatatgtacactgccatcataacaagttaccctaaatgcagctgccccaaccctgtaacaccatgcaagcatttgctgtttgtgtatatccaagtgttgggtctttcagcccatgataagtcttttagggagggctcattctcgcattgggagtggagcacatgctggggcgcgacacgctgcctgaatctcggtctcgggggtacttgctgccctatttgtttggatgagatggggaagcaagacaaggtggtggcttgtgggacgtgtagaaacccgatacatgaggaatgtttcttgaagtggaagaggagcgcgaggaagaagccaacttattgtgtgatgtgcagggcaagatggggttcgattcagcaggagcaggaaaagtacttgaacttggtggcttacgccagtactagcgaggaggaggaagatgatgagtacgaagacgagtatgaacgatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000209 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004706 GO:0004709 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006508 GO:0006511 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007254 GO:0007256 GO:0007346 GO:0008150 GO:0008152 GO:0008289 GO:0008432 GO:0009056 GO:0009057 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030163 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031434 GO:0032147 GO:0032231 GO:0032232 GO:0032268 GO:0032270 GO:0032446 GO:0032872 GO:0032874 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043632 GO:0043900 GO:0043902 GO:0043903 GO:0043933 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0046328 GO:0046330 GO:0046625 GO:0046777 GO:0046782 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048583 GO:0048584 GO:0050434 GO:0050789 GO:0050790 GO:0050792 GO:0050794 GO:0050896 GO:0051019 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051259 GO:0051338 GO:0051347 GO:0051403 GO:0051493 GO:0051494 GO:0051603 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0110053 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902531 GO:1902533 GO:1902680 GO:1902903 GO:1902904 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SWIM zf-RING_2

Protein Analysis

169

Amino Acids

19.35

Weight (kDa)

6.21

Isoelectric Point (pI)

52.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 142
AccBSI CCGCTC 1 cut(s) 9
AccII CGCG 2 cut(s) 218, 362
AciI CCGC 1 cut(s) 7
AfaI GTAC 5 cut(s) 53, 250, 434, 460, 488
AfiI CCNNNNNNNGG 1 cut(s) 142
AflIII ACRYGT 1 cut(s) 311
AgsI TTSAA 2 cut(s) 346, 439
AjiI CACGTC 1 cut(s) 312
AluBI AGCT 1 cut(s) 87
AluI AGCT 1 cut(s) 87
Alw21I GWGCWC 1 cut(s) 206
Alw26I GTCTC 1 cut(s) 245
Ama87I CYCGRG 1 cut(s) 242
ApeKI GCWGC 4 cut(s) 84, 87, 225, 255
AspLEI GCGC 2 cut(s) 218, 362
AvaI CYCGRG 1 cut(s) 242
BanII GRGCYC 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 498
Bbv12I GWGCWC 1 cut(s) 206
BbvI GCAGC 4 cut(s) 74, 96, 212, 242
BccI CCATC 3 cut(s) 68, 271, 396
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 1 cut(s) 462
BisI GCNGC 4 cut(s) 85, 88, 226, 256
BlsI GCNGC 4 cut(s) 86, 89, 227, 257
BmcAI AGTACT 2 cut(s) 434, 460
BmeT110I CYCGRG 1 cut(s) 242
BmgBI CACGTC 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 498
BsaI GGTCTC 1 cut(s) 245
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse1I ACTGG 3 cut(s) 12, 46, 456
BseGI GGATG 1 cut(s) 277
BseLI CCNNNNNNNGG 1 cut(s) 142
BseNI ACTGG 3 cut(s) 12, 46, 456
BseRI GAGGAG 3 cut(s) 370, 482, 485
BseXI GCAGC 4 cut(s) 74, 96, 212, 242
BseYI CCCAGC 1 cut(s) 210
BsgI GTGCAG 1 cut(s) 412
Bsh1236I CGCG 2 cut(s) 218, 362
BsiHKAI GWGCWC 1 cut(s) 206
BsiHKCI CYCGRG 1 cut(s) 242
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 322
Bso31I GGTCTC 1 cut(s) 245
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 2 cut(s) 182, 206
Bsp1407I TGTACA 1 cut(s) 51
BspACI CCGC 1 cut(s) 7
BspFNI CGCG 2 cut(s) 218, 362
BspTNI GGTCTC 1 cut(s) 245
BsrBI CCGCTC 1 cut(s) 9
BsrGI TGTACA 1 cut(s) 51
BsrI ACTGG 3 cut(s) 12, 46, 456
Bst6I CTCTTC 1 cut(s) 347
BstAUI TGTACA 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 113
BstF5I GGATG 1 cut(s) 277
BstFNI CGCG 2 cut(s) 218, 362
BstHHI GCGC 2 cut(s) 218, 362
BstMAI GTCTC 1 cut(s) 245
BstMWI GCNNNNNNNGC 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 210
BstUI CGCG 2 cut(s) 218, 362
BstV1I GCAGC 4 cut(s) 74, 96, 212, 242
BstV2I GAAGAC 1 cut(s) 498
BtrI CACGTC 1 cut(s) 312
BtsCI GGATG 1 cut(s) 277
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 3 cut(s) 19, 39, 54
Cac8I GCNNGC 1 cut(s) 113
CfoI GCGC 2 cut(s) 218, 362
Csp6I GTAC 5 cut(s) 52, 249, 433, 459, 487
CviAII CATG 4 cut(s) 108, 158, 207, 329
CviJI RGCY 6 cut(s) 87, 155, 180, 302, 373, 449
CviKI_1 RGCY 6 cut(s) 87, 155, 180, 302, 373, 449
CviQI GTAC 5 cut(s) 52, 249, 433, 459, 487
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
Eco24I GRGCYC 1 cut(s) 182
Eco31I GGTCTC 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 242
EcoT38I GRGCYC 1 cut(s) 182
FaeI CATG 4 cut(s) 111, 161, 210, 332
FaiI YATR 8 cut(s) 50, 65, 109, 132, 159, 208, 330, 501
FaqI GGGAC 1 cut(s) 322
FatI CATG 4 cut(s) 107, 157, 206, 328
Fnu4HI GCNGC 4 cut(s) 85, 88, 226, 256
FokI GGATG 1 cut(s) 284
FriOI GRGCYC 1 cut(s) 182
Fsp4HI GCNGC 4 cut(s) 85, 88, 226, 256
FspBI CTAG 1 cut(s) 462
GlaI GCGC 2 cut(s) 217, 361
GluI GCNGC 4 cut(s) 85, 88, 226, 256
GsaI CCCAGC 1 cut(s) 214
HhaI GCGC 2 cut(s) 218, 362
Hin1II CATG 4 cut(s) 111, 161, 210, 332
Hin6I GCGC 2 cut(s) 216, 360
HinP1I GCGC 2 cut(s) 216, 360
HinfI GANTC 3 cut(s) 36, 232, 412
Hpy166II GTNNAC 1 cut(s) 54
Hpy188III TCNNGA 1 cut(s) 343
Hpy8I GTNNAC 1 cut(s) 54
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 4 cut(s) 4, 84, 111, 393
HpyF10VI GCNNNNNNNGC 1 cut(s) 222
HpySE526I ACGT 1 cut(s) 311
Hsp92II CATG 4 cut(s) 111, 161, 210, 332
HspAI GCGC 2 cut(s) 216, 360
LmnI GCTCC 4 cut(s) 14, 201, 357, 421
LpnPI CCDG 9 cut(s) 25, 27, 111, 196, 242, 379, 404, 410, 469
Lsp1109I GCAGC 4 cut(s) 74, 96, 212, 242
MaeI CTAG 1 cut(s) 462
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 3 cut(s) 37, 71, 100
MbiI CCGCTC 1 cut(s) 9
MboII GAAGA 5 cut(s) 16, 364, 379, 488, 503
MhlI GDGCHC 2 cut(s) 182, 206
MlyI GAGTC 1 cut(s) 45
MnlI CCTC 7 cut(s) 169, 325, 348, 357, 460, 463, 466
MspA1I CMGCKG 1 cut(s) 87
MvnI CGCG 2 cut(s) 218, 362
MwoI GCNNNNNNNGC 1 cut(s) 222
NlaIII CATG 4 cut(s) 111, 161, 210, 332
NmuCI GTSAC 1 cut(s) 37
NspI RCATGY 1 cut(s) 210
PfeI GAWTC 2 cut(s) 232, 412
PflMI CCANNNNNTGG 1 cut(s) 142
PkrI GCNGC 4 cut(s) 86, 89, 227, 257
PleI GAGTC 1 cut(s) 44
PpsI GAGTC 1 cut(s) 44
PspFI CCCAGC 1 cut(s) 210
PvuII CAGCTG 1 cut(s) 87
RsaI GTAC 5 cut(s) 53, 250, 434, 460, 488
RsaNI GTAC 5 cut(s) 52, 249, 433, 459, 487
SatI GCNGC 4 cut(s) 85, 88, 226, 256
ScaI AGTACT 2 cut(s) 434, 460
SchI GAGTC 1 cut(s) 45
SduI GDGCHC 2 cut(s) 182, 206
SetI ASST 3 cut(s) 89, 297, 314
SsiI CCGC 1 cut(s) 7
SspMI CTAG 1 cut(s) 462
TaiI ACGT 1 cut(s) 314
TaqI TCGA 1 cut(s) 410
TaqII GACCGA 1 cut(s) 227
TatI WGTACW 3 cut(s) 51, 432, 458
TfiI GAWTC 2 cut(s) 232, 412
TscAI CASTG 3 cut(s) 19, 46, 61
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 4 cut(s) 84, 87, 225, 255
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 16
TspRI CASTG 3 cut(s) 19, 46, 61
Van91I CCANNNNNTGG 1 cut(s) 142
XceI RCATGY 1 cut(s) 210
XspI CTAG 1 cut(s) 462
ZrmI AGTACT 2 cut(s) 434, 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.