Rh6DG461700

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0004740
Physical Location & Seq
Reverse (-)
63114714 .. 63115776
1063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG461700.1

Sequence Viewer

Length: 654 bp
ATGTCAAAATTGAGTTCATGGGACGGGGGAGGGTTAATGTCGACATCAAAGAGTGTGTTGGTGGTCAGTGCGATAGGATTCACTTCAGATGGGTTTAGATCATTGCACGGGTCCGTCGTTGGCTCTAGTGGTGGCGGCGGCAACCTTTGTACCGTAACAACTACGGGCAATCATGGATTTCTCGGAGGCTCTGTGCATGGAGGTGCGACCGGGGCTTGGCTTCCTAATTCCCCCAATTTAACAATACTGCTGTCCAGCAGCTACAAGCCCTTCAATGACCGTGTGGAGCAAGCCCTCAACCATCACATTCGCCTCCTGCACCGCTCCAGTGCCACCTTCTTCATCTTAGGCGTCACTGGAAATGTGTACACTGCTATCATAACAAGTTACCTTAAATGCAGCTGCCCCGACCCTGTAACACCATGCAAGCATTTGTTGTTTGTGTATATCCAAGTGTTGGGTCTTTCAGCCCATGATAGGTCTCTTAGGGACGACTCATCCTCACTTTGGGAGTGGAGCACATGCTGGGGCGCGACACGCTGCCTGAATCTCGGGGTGGAGAAAACGTGCCGCAATAGTTTCATCAGTTGTATGATTTTCAGGGGAAGCAGTGGCAGTAGGGGTCTTCTTCGTCGTCAAGGCCAAGAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000209 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004706 GO:0004709 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006508 GO:0006511 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007254 GO:0007256 GO:0007346 GO:0008150 GO:0008152 GO:0008289 GO:0008432 GO:0009056 GO:0009057 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030163 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031434 GO:0032147 GO:0032231 GO:0032232 GO:0032268 GO:0032270 GO:0032446 GO:0032872 GO:0032874 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043632 GO:0043900 GO:0043902 GO:0043903 GO:0043933 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0046328 GO:0046330 GO:0046625 GO:0046777 GO:0046782 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048583 GO:0048584 GO:0050434 GO:0050789 GO:0050790 GO:0050792 GO:0050794 GO:0050896 GO:0051019 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051259 GO:0051338 GO:0051347 GO:0051403 GO:0051493 GO:0051494 GO:0051603 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0110053 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902531 GO:1902533 GO:1902680 GO:1902903 GO:1902904 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SWIM zf-RING_2

Protein Analysis

217

Amino Acids

23.26

Weight (kDa)

9.16

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 457
AccBSI CCGCTC 1 cut(s) 324
AccI GTMKAC 1 cut(s) 41
AccII CGCG 1 cut(s) 533
AciI CCGC 4 cut(s) 135, 138, 322, 571
AcuI CTGAAG 1 cut(s) 69
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 2 cut(s) 151, 368
AfiI CCNNNNNNNGG 4 cut(s) 216, 457, 477, 507
AgsI TTSAA 1 cut(s) 274
AluBI AGCT 2 cut(s) 261, 402
AluI AGCT 2 cut(s) 261, 402
Alw21I GWGCWC 1 cut(s) 521
Alw26I GTCTC 1 cut(s) 486
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 1 cut(s) 640
ApeKI GCWGC 4 cut(s) 258, 399, 402, 540
AspLEI GCGC 1 cut(s) 533
AspS9I GGNCC 1 cut(s) 111
AsuC2I CCSGG 1 cut(s) 211
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 1 cut(s) 111
BaeI ACNNNNGTAYC 2 cut(s) 133, 166
BbsI GAAGAC 1 cut(s) 617
Bbv12I GWGCWC 1 cut(s) 521
BbvI GCAGC 4 cut(s) 270, 389, 411, 527
BccI CCATC 2 cut(s) 83, 309
BcnI CCSGG 1 cut(s) 211
BcoDI GTCTC 1 cut(s) 486
BfaI CTAG 1 cut(s) 126
BisI GCNGC 7 cut(s) 136, 139, 259, 400, 403, 541, 571
BlsI GCNGC 7 cut(s) 137, 140, 260, 401, 404, 542, 572
Bme1390I CCNGG 1 cut(s) 211
Bme18I GGWCC 1 cut(s) 111
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 1 cut(s) 111
BmiI GGNNCC 1 cut(s) 112
BmrFI CCNGG 1 cut(s) 211
BpiI GAAGAC 1 cut(s) 617
BpmI CTGGAG 1 cut(s) 310
BpuMI CCSGG 1 cut(s) 211
BsaHI GRCGYC 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 486
BsaJI CCNNGG 1 cut(s) 210
BsaXI ACNNNNNCTCC 4 cut(s) 177, 207, 551, 581
Bsc4I CCNNNNNNNGG 4 cut(s) 216, 457, 477, 507
Bse1I ACTGG 2 cut(s) 327, 361
Bse3DI GCAATG 1 cut(s) 101
BseDI CCNNGG 1 cut(s) 210
BseGI GGATG 1 cut(s) 497
BseLI CCNNNNNNNGG 4 cut(s) 216, 457, 477, 507
BseMI GCAATG 1 cut(s) 101
BseNI ACTGG 2 cut(s) 327, 361
BseXI GCAGC 4 cut(s) 270, 389, 411, 527
BseYI CCCAGC 1 cut(s) 525
BsgI GTGCAG 1 cut(s) 302
Bsh1236I CGCG 1 cut(s) 533
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 1 cut(s) 642
BsiEI CGRYCG 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 521
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 2 cut(s) 35, 503
BslI CCNNNNNNNGG 4 cut(s) 216, 457, 477, 507
BsmAI GTCTC 1 cut(s) 486
BsmFI GGGAC 2 cut(s) 35, 503
BsnI GGCC 1 cut(s) 642
Bso31I GGTCTC 1 cut(s) 486
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 521
Bsp1407I TGTACA 1 cut(s) 366
Bsp143I GATC 1 cut(s) 98
BspACI CCGC 4 cut(s) 135, 138, 322, 571
BspANI GGCC 1 cut(s) 642
BspFNI CGCG 1 cut(s) 533
BspLI GGNNCC 1 cut(s) 112
BspTNI GGTCTC 1 cut(s) 486
BsrBI CCGCTC 1 cut(s) 324
BsrDI GCAATG 1 cut(s) 101
BsrGI TGTACA 1 cut(s) 366
BsrI ACTGG 2 cut(s) 327, 361
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 1 cut(s) 98
BssNI GRCGYC 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 154, 281
BstACI GRCGYC 1 cut(s) 351
BstAUI TGTACA 1 cut(s) 366
BstC8I GCNNGC 2 cut(s) 291, 428
BstDEI CTNAG 2 cut(s) 346, 485
BstF5I GGATG 1 cut(s) 497
BstFNI CGCG 1 cut(s) 533
BstHHI GCGC 1 cut(s) 533
BstKTI GATC 1 cut(s) 101
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 1 cut(s) 98
BstMCI CGRYCG 1 cut(s) 210
BstMWI GCNNNNNNNGC 2 cut(s) 212, 537
BstNSI RCATGY 1 cut(s) 525
BstSCI CCNGG 1 cut(s) 209
BstUI CGCG 1 cut(s) 533
BstV1I GCAGC 4 cut(s) 270, 389, 411, 527
BstV2I GAAGAC 1 cut(s) 617
BsuRI GGCC 1 cut(s) 642
BtsCI GGATG 1 cut(s) 497
BtsI GCAGTG 2 cut(s) 369, 616
BtsIMutI CAGTG 5 cut(s) 73, 334, 354, 369, 616
Cac8I GCNNGC 2 cut(s) 291, 428
CfoI GCGC 1 cut(s) 533
Cfr13I GGNCC 1 cut(s) 111
CseI GACGC 1 cut(s) 340
Csp6I GTAC 2 cut(s) 150, 367
CviAII CATG 6 cut(s) 18, 173, 197, 423, 473, 522
CviQI GTAC 2 cut(s) 150, 367
DdeI CTNAG 2 cut(s) 346, 485
DpnI GATC 1 cut(s) 100
DpnII GATC 1 cut(s) 98
Eco31I GGTCTC 1 cut(s) 486
Eco47I GGWCC 1 cut(s) 111
Eco57I CTGAAG 1 cut(s) 69
Eco88I CYCGRG 1 cut(s) 551
FaeI CATG 6 cut(s) 21, 176, 200, 426, 476, 525
FaiI YATR 9 cut(s) 19, 174, 198, 380, 424, 447, 474, 523, 593
FaqI GGGAC 2 cut(s) 35, 503
FatI CATG 6 cut(s) 17, 172, 196, 422, 472, 521
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 7 cut(s) 136, 139, 259, 400, 403, 541, 571
FokI GGATG 1 cut(s) 484
Fsp4HI GCNGC 7 cut(s) 136, 139, 259, 400, 403, 541, 571
FspBI CTAG 1 cut(s) 126
GlaI GCGC 1 cut(s) 532
GluI GCNGC 7 cut(s) 136, 139, 259, 400, 403, 541, 571
GsaI CCCAGC 1 cut(s) 529
GsuI CTGGAG 1 cut(s) 310
HaeIII GGCC 1 cut(s) 642
HapII CCGG 1 cut(s) 210
HgaI GACGC 1 cut(s) 340
HhaI GCGC 1 cut(s) 533
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 6 cut(s) 21, 176, 200, 426, 476, 525
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HincII GTYRAC 1 cut(s) 42
HindII GTYRAC 1 cut(s) 42
HinfI GANTC 3 cut(s) 78, 494, 547
HpaII CCGG 1 cut(s) 210
Hpy166II GTNNAC 3 cut(s) 42, 367, 369
Hpy188I TCNGA 2 cut(s) 88, 185
Hpy8I GTNNAC 3 cut(s) 42, 367, 369
Hpy99I CGWCG 2 cut(s) 119, 636
HpyAV CCTTC 2 cut(s) 280, 346
HpyCH4III ACNGT 2 cut(s) 154, 281
HpyCH4IV ACGT 1 cut(s) 566
HpyCH4V TGCA 5 cut(s) 106, 196, 319, 399, 426
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 537
HpyF3I CTNAG 2 cut(s) 346, 485
HpySE526I ACGT 1 cut(s) 566
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 6 cut(s) 21, 176, 200, 426, 476, 525
HspAI GCGC 1 cut(s) 531
Kzo9I GATC 1 cut(s) 98
LmnI GCTCC 3 cut(s) 286, 329, 516
LpnPI CCDG 9 cut(s) 223, 268, 329, 340, 342, 426, 511, 557, 586
Lsp1109I GCAGC 4 cut(s) 270, 389, 411, 527
MaeI CTAG 1 cut(s) 126
MaeII ACGT 1 cut(s) 566
MaeIII GTNAC 4 cut(s) 154, 352, 386, 415
MalI GATC 1 cut(s) 100
MbiI CCGCTC 1 cut(s) 324
MboI GATC 1 cut(s) 98
MboII GAAGA 3 cut(s) 331, 617, 620
MhlI GDGCHC 1 cut(s) 521
MluCI AATT 3 cut(s) 8, 226, 235
MlyI GAGTC 1 cut(s) 488
MnlI CCTC 6 cut(s) 23, 179, 194, 305, 323, 511
MseI TTAA 3 cut(s) 35, 239, 393
MslI CAYNNNNRTG 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 402
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 211
MvnI CGCG 1 cut(s) 533
MwoI GCNNNNNNNGC 2 cut(s) 212, 537
NciI CCSGG 1 cut(s) 211
NdeII GATC 1 cut(s) 98
NlaIII CATG 6 cut(s) 21, 176, 200, 426, 476, 525
NlaIV GGNNCC 1 cut(s) 112
NmuCI GTSAC 1 cut(s) 352
NspI RCATGY 1 cut(s) 525
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PfeI GAWTC 2 cut(s) 78, 547
PflMI CCANNNNNTGG 1 cut(s) 457
PkrI GCNGC 7 cut(s) 137, 140, 260, 401, 404, 542, 572
PleI GAGTC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 488
PspFI CCCAGC 1 cut(s) 525
PspN4I GGNNCC 1 cut(s) 112
PspPI GGNCC 1 cut(s) 111
PvuII CAGCTG 1 cut(s) 402
RsaI GTAC 2 cut(s) 151, 368
RsaNI GTAC 2 cut(s) 150, 367
RseI CAYNNNNRTG 1 cut(s) 201
SalI GTCGAC 1 cut(s) 40
SaqAI TTAA 3 cut(s) 35, 239, 393
SatI GCNGC 7 cut(s) 136, 139, 259, 400, 403, 541, 571
Sau3AI GATC 1 cut(s) 98
Sau96I GGNCC 1 cut(s) 111
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 1 cut(s) 211
SduI GDGCHC 1 cut(s) 521
SetI ASST 8 cut(s) 147, 205, 263, 338, 393, 404, 482, 569
SinI GGWCC 1 cut(s) 111
SmiMI CAYNNNNRTG 1 cut(s) 201
Sse9I AATT 3 cut(s) 8, 226, 235
SsiI CCGC 4 cut(s) 135, 138, 322, 571
SspMI CTAG 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 209
TaaI ACNGT 2 cut(s) 154, 281
TaiI ACGT 1 cut(s) 569
TaqI TCGA 1 cut(s) 41
TasI AATT 3 cut(s) 8, 226, 235
TatI WGTACW 1 cut(s) 366
TauI GCSGC 3 cut(s) 138, 141, 573
TfiI GAWTC 2 cut(s) 78, 547
Tru1I TTAA 3 cut(s) 35, 239, 393
Tru9I TTAA 3 cut(s) 35, 239, 393
TscAI CASTG 5 cut(s) 73, 334, 361, 376, 616
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 4 cut(s) 258, 399, 402, 540
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 3 cut(s) 6, 331, 571
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 5 cut(s) 73, 334, 361, 376, 616
Van91I CCANNNNNTGG 1 cut(s) 457
VpaK11BI GGWCC 1 cut(s) 111
XceI RCATGY 1 cut(s) 525
XmiI GTMKAC 1 cut(s) 41
XspI CTAG 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.