Rh6BG440000

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004740
Physical Location & Seq
Forward (+)
65818860 .. 65819509
650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG440000.1

Sequence Viewer

Length: 498 bp
ATGTTGCGGTGGCGCAACCCGGACAAACCTTTCGAAGACCGGGTGGAGCGAGCCATCCACCAGCACATCCGCCTCCTGCACCGCTCGGGTTCCAGGAGGAGCGCACTCCATCCGAGCGAGGTGAGCTGGCTGTTGGGGCTGCCCATGTCACGCGCATCACTGGCTGAGGATAGTGTCCGACACTGGTTTCATCAGATCCATCCTCTTGGAAAGAAACAGCAGCAGCAGGAAAGGTATTTCTCATCGAGGCCAAGCCTATGTCTGCGAATTGAAGATGGTAGCTACTGCCCTGTTTGCTTGGATGAGATAAGAAAGAGAGATAAAGCGGTGCATTGTGGGCAATGTAGGAATCCAATGCATGAGGAATGCTTCTTGAAGTGGGGGAGGAGCGCAAGGAACAAGTCGCCTCATTGTGCAATATGCAGTGCAAAATGGAGCAGTACAATCAGCCGCGAGCAGGCGCAACACATGTACTTGAATTTAGCTGCTTACGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000209 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004706 GO:0004709 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006508 GO:0006511 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007254 GO:0007256 GO:0007346 GO:0008150 GO:0008152 GO:0008289 GO:0008432 GO:0009056 GO:0009057 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030163 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031434 GO:0032147 GO:0032231 GO:0032232 GO:0032268 GO:0032270 GO:0032446 GO:0032872 GO:0032874 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043632 GO:0043900 GO:0043902 GO:0043903 GO:0043933 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0046328 GO:0046330 GO:0046625 GO:0046777 GO:0046782 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048583 GO:0048584 GO:0050434 GO:0050789 GO:0050790 GO:0050792 GO:0050794 GO:0050896 GO:0051019 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051259 GO:0051338 GO:0051347 GO:0051403 GO:0051493 GO:0051494 GO:0051603 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0110053 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902531 GO:1902533 GO:1902680 GO:1902903 GO:1902904 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SWIM zf-RING_2

Protein Analysis

165

Amino Acids

19.4

Weight (kDa)

9.5

Isoelectric Point (pI)

82.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prok-RING_1 PF14446 93 - 136 2.7e-06 Prokaryotic RING finger family 1
zf-RING_2 PF13639 96 - 141 5.7e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 84
AccII CGCG 2 cut(s) 153, 453
AciI CCGC 5 cut(s) 7, 70, 82, 326, 451
AclWI GGATC 1 cut(s) 190
AcsI RAATTY 1 cut(s) 478
AfaI GTAC 2 cut(s) 442, 473
AfiI CCNNNNNNNGG 1 cut(s) 457
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 3 cut(s) 272, 376, 478
AjnI CCWGG 1 cut(s) 92
AluBI AGCT 3 cut(s) 126, 282, 485
AluI AGCT 3 cut(s) 126, 282, 485
AlwI GGATC 1 cut(s) 190
Ama87I CYCGRG 1 cut(s) 85
AoxI GGCC 1 cut(s) 248
ApeKI GCWGC 4 cut(s) 139, 220, 223, 485
ApoI RAATTY 1 cut(s) 478
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
AspLEI GCGC 5 cut(s) 15, 104, 155, 392, 463
AsuC2I CCSGG 2 cut(s) 20, 41
AsuHPI GGTGA 1 cut(s) 133
AsuII TTCGAA 1 cut(s) 33
AvaI CYCGRG 1 cut(s) 85
BbsI GAAGAC 1 cut(s) 42
BbvCI CCTCAGC 1 cut(s) 165
BbvI GCAGC 4 cut(s) 126, 232, 235, 472
BccI CCATC 4 cut(s) 62, 117, 207, 269
BciT130I CCWGG 1 cut(s) 94
BcnI CCSGG 2 cut(s) 20, 41
BfaI CTAG 1 cut(s) 496
BisI GCNGC 5 cut(s) 140, 221, 224, 451, 486
BlsI GCNGC 5 cut(s) 141, 222, 225, 452, 487
Bme1390I CCNGG 3 cut(s) 20, 41, 94
BmeT110I CYCGRG 1 cut(s) 85
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 3 cut(s) 20, 41, 94
BmsI GCATC 1 cut(s) 164
BpiI GAAGAC 1 cut(s) 42
Bpu10I CCTNAGC 1 cut(s) 165
Bpu14I TTCGAA 1 cut(s) 33
BpuMI CCSGG 2 cut(s) 20, 41
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse1I ACTGG 2 cut(s) 165, 188
Bse3DI GCAATG 1 cut(s) 347
BseBI CCWGG 1 cut(s) 94
BseGI GGATG 5 cut(s) 54, 66, 109, 199, 307
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMI GCAATG 1 cut(s) 347
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 2 cut(s) 165, 188
BseRI GAGGAG 2 cut(s) 112, 400
BseXI GCAGC 4 cut(s) 126, 232, 235, 472
BsgI GTGCAG 1 cut(s) 62
Bsh1236I CGCG 2 cut(s) 153, 453
BshFI GGCC 1 cut(s) 250
BsiHKCI CYCGRG 1 cut(s) 85
BsiSI CCGG 2 cut(s) 20, 40
BslI CCNNNNNNNGG 1 cut(s) 457
BsmI GAATGC 1 cut(s) 371
BsnI GGCC 1 cut(s) 250
BsoBI CYCGRG 1 cut(s) 85
Bsp119I TTCGAA 1 cut(s) 33
Bsp143I GATC 1 cut(s) 195
BspACI CCGC 5 cut(s) 7, 70, 82, 326, 451
BspANI GGCC 1 cut(s) 250
BspCNI CTCAG 1 cut(s) 157
BspFNI CGCG 2 cut(s) 153, 453
BspLI GGNNCC 1 cut(s) 91
BspPI GGATC 1 cut(s) 190
BspT104I TTCGAA 1 cut(s) 33
BsrBI CCGCTC 1 cut(s) 84
BsrDI GCAATG 1 cut(s) 347
BsrI ACTGG 2 cut(s) 165, 188
BssMI GATC 1 cut(s) 195
Bst2UI CCWGG 1 cut(s) 94
BstBI TTCGAA 1 cut(s) 33
BstC8I GCNNGC 4 cut(s) 51, 128, 455, 459
BstDEI CTNAG 1 cut(s) 165
BstF5I GGATG 5 cut(s) 54, 66, 109, 199, 307
BstFNI CGCG 2 cut(s) 153, 453
BstHHI GCGC 5 cut(s) 15, 104, 155, 392, 463
BstKTI GATC 1 cut(s) 198
BstMBI GATC 1 cut(s) 195
BstMWI GCNNNNNNNGC 5 cut(s) 123, 136, 161, 294, 337
BstNI CCWGG 1 cut(s) 94
BstNSI RCATGY 1 cut(s) 472
BstSCI CCNGG 3 cut(s) 18, 39, 92
BstUI CGCG 2 cut(s) 153, 453
BstV1I GCAGC 4 cut(s) 126, 232, 235, 472
BstV2I GAAGAC 1 cut(s) 42
BstX2I RGATCY 1 cut(s) 195
BstXI CCANNNNNNTGG 1 cut(s) 206
BstYI RGATCY 1 cut(s) 195
BsuRI GGCC 1 cut(s) 250
BtsCI GGATG 5 cut(s) 54, 66, 109, 199, 307
BtsI GCAGTG 1 cut(s) 430
BtsIMutI CAGTG 3 cut(s) 158, 181, 430
Cac8I GCNNGC 4 cut(s) 51, 128, 455, 459
CfoI GCGC 5 cut(s) 15, 104, 155, 392, 463
Csp6I GTAC 2 cut(s) 441, 472
CviAII CATG 3 cut(s) 145, 359, 469
CviQI GTAC 2 cut(s) 441, 472
DdeI CTNAG 1 cut(s) 165
DpnI GATC 1 cut(s) 197
DpnII GATC 1 cut(s) 195
EciI GGCGGA 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 92
EcoT22I ATGCAT 1 cut(s) 360
FaeI CATG 3 cut(s) 148, 362, 472
FaiI YATR 5 cut(s) 146, 259, 360, 421, 470
FatI CATG 3 cut(s) 144, 358, 468
Fnu4HI GCNGC 5 cut(s) 140, 221, 224, 451, 486
FokI GGATG 5 cut(s) 41, 53, 96, 186, 314
Fsp4HI GCNGC 5 cut(s) 140, 221, 224, 451, 486
FspBI CTAG 1 cut(s) 496
GlaI GCGC 5 cut(s) 14, 103, 154, 391, 462
GluI GCNGC 5 cut(s) 140, 221, 224, 451, 486
HaeIII GGCC 1 cut(s) 250
HapII CCGG 2 cut(s) 20, 40
HhaI GCGC 5 cut(s) 15, 104, 155, 392, 463
Hin1II CATG 3 cut(s) 148, 362, 472
Hin6I GCGC 5 cut(s) 13, 102, 153, 390, 461
HinP1I GCGC 5 cut(s) 13, 102, 153, 390, 461
HinfI GANTC 1 cut(s) 349
HpaII CCGG 2 cut(s) 20, 40
HphI GGTGA 1 cut(s) 133
Hpy188I TCNGA 3 cut(s) 114, 179, 195
Hpy188III TCNNGA 1 cut(s) 373
HpyCH4V TGCA 6 cut(s) 79, 331, 358, 416, 423, 428
HpyF10VI GCNNNNNNNGC 5 cut(s) 123, 136, 161, 294, 337
HpyF3I CTNAG 1 cut(s) 165
Hsp92II CATG 3 cut(s) 148, 362, 472
HspAI GCGC 5 cut(s) 13, 102, 153, 390, 461
Kzo9I GATC 1 cut(s) 195
LmnI GCTCC 4 cut(s) 46, 99, 387, 435
Lsp1109I GCAGC 4 cut(s) 126, 232, 235, 472
LweI GCATC 1 cut(s) 164
MaeI CTAG 1 cut(s) 496
MaeIII GTNAC 1 cut(s) 147
MalI GATC 1 cut(s) 197
MbiI CCGCTC 1 cut(s) 84
MboI GATC 1 cut(s) 195
MboII GAAGA 2 cut(s) 47, 284
MflI RGATCY 1 cut(s) 195
MluCI AATT 2 cut(s) 267, 478
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 9 cut(s) 83, 90, 112, 160, 213, 240, 355, 378, 417
Mph1103I ATGCAT 1 cut(s) 360
MspI CCGG 2 cut(s) 20, 40
MspR9I CCNGG 3 cut(s) 20, 41, 94
Mva1269I GAATGC 1 cut(s) 371
MvaI CCWGG 1 cut(s) 94
MvnI CGCG 2 cut(s) 153, 453
MwoI GCNNNNNNNGC 5 cut(s) 123, 136, 161, 294, 337
NciI CCSGG 2 cut(s) 20, 41
NdeII GATC 1 cut(s) 195
NlaIII CATG 3 cut(s) 148, 362, 472
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 147
NsiI ATGCAT 1 cut(s) 360
NspI RCATGY 1 cut(s) 472
NspV TTCGAA 1 cut(s) 33
PciI ACATGT 1 cut(s) 468
PctI GAATGC 1 cut(s) 371
PfeI GAWTC 1 cut(s) 349
PfoI TCCNGGA 1 cut(s) 92
PkrI GCNGC 5 cut(s) 141, 222, 225, 452, 487
PscI ACATGT 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 92
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 1 cut(s) 91
PsuI RGATCY 1 cut(s) 195
RsaI GTAC 2 cut(s) 442, 473
RsaNI GTAC 2 cut(s) 441, 472
SatI GCNGC 5 cut(s) 140, 221, 224, 451, 486
Sau3AI GATC 1 cut(s) 195
ScrFI CCNGG 3 cut(s) 20, 41, 94
SetI ASST 6 cut(s) 31, 123, 128, 236, 284, 487
SfaNI GCATC 1 cut(s) 164
SfuI TTCGAA 1 cut(s) 33
Sse9I AATT 2 cut(s) 267, 478
SsiI CCGC 5 cut(s) 7, 70, 82, 326, 451
SspMI CTAG 1 cut(s) 496
StyD4I CCNGG 3 cut(s) 18, 39, 92
TaqI TCGA 2 cut(s) 33, 245
TasI AATT 2 cut(s) 267, 478
TatI WGTACW 2 cut(s) 440, 471
TauI GCSGC 1 cut(s) 453
TfiI GAWTC 1 cut(s) 349
TscAI CASTG 3 cut(s) 165, 188, 430
TseFI GTSAC 1 cut(s) 147
TseI GCWGC 4 cut(s) 139, 220, 223, 485
Tsp45I GTSAC 1 cut(s) 147
TspDTI ATGAA 1 cut(s) 179
TspRI CASTG 3 cut(s) 165, 188, 430
XapI RAATTY 1 cut(s) 478
XceI RCATGY 1 cut(s) 472
XspI CTAG 1 cut(s) 496
Zsp2I ATGCAT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.