RLG00000010801

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004740
Physical Location & Seq
Forward (+)
4443349 .. 4443993
645 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010801

Sequence Viewer

Length: 645 bp
ATGCCGCGGTGGCGCAACCCGTACAAACCTTTCGAAGACCGGGTGGAGATAGCCATCCGCCTCCTGCATCGCTCTGGTTCCAGGTTCTTCGTTTTAGGACACACCAAAAATGTGTACACCGTCACATTGTCCACCGAGTCAACATGCTCGTGCCCTGACGTTGTCTCCCCCTGCAAGCACCTGCTGTTTGTGTATCTCCAAGTATTGGGTGTTTGTATCGACACGCGTTGTGTTAGGAGGAGCGCACCCCACCCGAGCGAGGTGAGCTGGCTGTTGGGGCTGCCCATGTCGCGAGCATCACTGGCTGAGGATAGTGTTCAACAGTGGTTTCATCAGCTCCATCCTCTGGGAAAGAAGCAGCAGCAGCAGCAGGAGGATAGGTCTTTCTCATCAAGGCCAAGCCTAAGTCTGCGACTTGAAGACAGTAGCGGCTGCCCTGTTTGCTTGGATGAGATAAGAAAGAGAGACAAAGCAGTGCATTGTAGGAAATGTAGGAATCCAATGCATGAGGAATGTTTCTTGAAGTGGGGGAGGAGGGCAAGGAACAAGTCGCCTCATTGTGCAATATGCAGTGCAAAATGGAGCAGCACAATCAGCCGCGAGCAGGCGCAGCACATGTGCTTGAATTTAGCTGCTTACGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000209 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004706 GO:0004709 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006508 GO:0006511 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007254 GO:0007256 GO:0007346 GO:0008150 GO:0008152 GO:0008289 GO:0008432 GO:0009056 GO:0009057 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030163 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031434 GO:0032147 GO:0032231 GO:0032232 GO:0032268 GO:0032270 GO:0032446 GO:0032872 GO:0032874 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043632 GO:0043900 GO:0043902 GO:0043903 GO:0043933 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0046328 GO:0046330 GO:0046625 GO:0046777 GO:0046782 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048583 GO:0048584 GO:0050434 GO:0050789 GO:0050790 GO:0050792 GO:0050794 GO:0050896 GO:0051019 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051259 GO:0051338 GO:0051347 GO:0051403 GO:0051493 GO:0051494 GO:0051603 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0110053 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902531 GO:1902533 GO:1902680 GO:1902903 GO:1902904 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SWIM zf-RING_2

Protein Analysis

215

Amino Acids

24.68

Weight (kDa)

9.28

Isoelectric Point (pI)

73.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIM PF04434 39 - 67 7.1e-06 SWIM zinc finger
Prok-RING_1 PF14446 144 - 181 6.6e-06 Prokaryotic RING finger family 1
zf-RING_2 PF13639 145 - 190 4.7e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 189
Acc36I ACCTGC 1 cut(s) 189
AccB7I CCANNNNNTGG 2 cut(s) 205, 346
AccII CGCG 4 cut(s) 7, 226, 292, 600
AciI CCGC 5 cut(s) 5, 7, 58, 429, 598
AcsI RAATTY 1 cut(s) 625
AfaI GTAC 2 cut(s) 23, 116
AfiI CCNNNNNNNGG 4 cut(s) 205, 259, 346, 604
AflIII ACRYGT 2 cut(s) 224, 615
AgsI TTSAA 4 cut(s) 320, 419, 523, 625
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 3 cut(s) 267, 337, 632
AluI AGCT 3 cut(s) 267, 337, 632
Alw26I GTCTC 2 cut(s) 169, 459
Ama87I CYCGRG 1 cut(s) 253
AoxI GGCC 1 cut(s) 395
ApeKI GCWGC 9 cut(s) 280, 358, 361, 364, 367, 432, 585, 610, 632
ApoI RAATTY 1 cut(s) 625
AspLEI GCGC 3 cut(s) 15, 245, 610
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 1 cut(s) 274
AsuII TTCGAA 1 cut(s) 33
AvaI CYCGRG 1 cut(s) 253
BaeGI GKGCMC 1 cut(s) 155
BauI CACGAG 1 cut(s) 148
BbsI GAAGAC 2 cut(s) 42, 426
BbvCI CCTCAGC 1 cut(s) 306
BbvI GCAGC 9 cut(s) 267, 370, 373, 376, 379, 419, 597, 619, 622
BccI CCATC 2 cut(s) 62, 348
BciT130I CCWGG 1 cut(s) 82
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 2 cut(s) 169, 459
BfaI CTAG 1 cut(s) 643
BfuAI ACCTGC 1 cut(s) 189
BglI GCCNNNNNGGC 1 cut(s) 10
Bme1390I CCNGG 2 cut(s) 41, 82
BmeT110I CYCGRG 1 cut(s) 253
BmiI GGNNCC 1 cut(s) 79
BmrFI CCNGG 2 cut(s) 41, 82
BmsI GCATC 2 cut(s) 76, 305
BpiI GAAGAC 2 cut(s) 42, 426
Bpu10I CCTNAGC 1 cut(s) 306
Bpu14I TTCGAA 1 cut(s) 33
BpuMI CCSGG 1 cut(s) 41
BsaBI GATNNNNATC 1 cut(s) 53
BsaJI CCNNGG 1 cut(s) 5
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 365, 395
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 259, 346, 604
Bse1I ACTGG 1 cut(s) 306
Bse8I GATNNNNATC 1 cut(s) 53
BseBI CCWGG 1 cut(s) 82
BseDI CCNNGG 1 cut(s) 5
BseGI GGATG 3 cut(s) 54, 340, 454
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 4 cut(s) 205, 259, 346, 604
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 1 cut(s) 306
BseRI GAGGAG 2 cut(s) 253, 547
BseSI GKGCMC 1 cut(s) 155
BseXI GCAGC 9 cut(s) 267, 370, 373, 376, 379, 419, 597, 619, 622
Bsh1236I CGCG 4 cut(s) 7, 226, 292, 600
BshFI GGCC 1 cut(s) 397
BsiHKCI CYCGRG 1 cut(s) 253
BsiSI CCGG 1 cut(s) 40
BslI CCNNNNNNNGG 4 cut(s) 205, 259, 346, 604
BsmAI GTCTC 2 cut(s) 169, 459
BsnI GGCC 1 cut(s) 397
BsoBI CYCGRG 1 cut(s) 253
Bsp119I TTCGAA 1 cut(s) 33
Bsp1286I GDGCHC 1 cut(s) 155
Bsp1407I TGTACA 1 cut(s) 114
Bsp68I TCGCGA 1 cut(s) 292
BspACI CCGC 5 cut(s) 5, 7, 58, 429, 598
BspANI GGCC 1 cut(s) 397
BspCNI CTCAG 1 cut(s) 298
BspFNI CGCG 4 cut(s) 7, 226, 292, 600
BspLI GGNNCC 1 cut(s) 79
BspMI ACCTGC 1 cut(s) 189
BspT104I TTCGAA 1 cut(s) 33
BsrGI TGTACA 1 cut(s) 114
BsrI ACTGG 1 cut(s) 306
BssECI CCNNGG 1 cut(s) 5
BssSI CACGAG 1 cut(s) 148
Bst2BI CACGAG 1 cut(s) 148
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 3 cut(s) 121, 324, 425
BstAUI TGTACA 1 cut(s) 114
BstBI TTCGAA 1 cut(s) 33
BstC8I GCNNGC 5 cut(s) 176, 269, 294, 602, 606
BstDEI CTNAG 2 cut(s) 306, 404
BstDSI CCRYGG 1 cut(s) 5
BstF5I GGATG 3 cut(s) 54, 340, 454
BstFNI CGCG 4 cut(s) 7, 226, 292, 600
BstHHI GCGC 3 cut(s) 15, 245, 610
BstMAI GTCTC 2 cut(s) 169, 459
BstNI CCWGG 1 cut(s) 82
BstNSI RCATGY 2 cut(s) 147, 619
BstSCI CCNGG 2 cut(s) 39, 80
BstSLI GKGCMC 1 cut(s) 155
BstUI CGCG 4 cut(s) 7, 226, 292, 600
BstV1I GCAGC 9 cut(s) 267, 370, 373, 376, 379, 419, 597, 619, 622
BstV2I GAAGAC 2 cut(s) 42, 426
BsuRI GGCC 1 cut(s) 397
BtgI CCRYGG 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 53
BtsCI GGATG 3 cut(s) 54, 340, 454
BtsI GCAGTG 2 cut(s) 480, 577
BtsIMutI CAGTG 4 cut(s) 299, 329, 480, 577
BtuMI TCGCGA 1 cut(s) 292
BveI ACCTGC 1 cut(s) 189
Cac8I GCNNGC 5 cut(s) 176, 269, 294, 602, 606
CfoI GCGC 3 cut(s) 15, 245, 610
Cfr42I CCGCGG 1 cut(s) 8
Csp6I GTAC 2 cut(s) 22, 115
CviAII CATG 4 cut(s) 144, 286, 506, 616
CviQI GTAC 2 cut(s) 22, 115
DdeI CTNAG 2 cut(s) 306, 404
EciI GGCGGA 1 cut(s) 47
Eco88I CYCGRG 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 80
EcoT22I ATGCAT 1 cut(s) 507
FaeI CATG 4 cut(s) 147, 289, 509, 619
FaiI YATR 5 cut(s) 145, 287, 507, 568, 617
FatI CATG 4 cut(s) 143, 285, 505, 615
FokI GGATG 3 cut(s) 41, 327, 461
FspBI CTAG 1 cut(s) 643
GlaI GCGC 3 cut(s) 14, 244, 609
HaeIII GGCC 1 cut(s) 397
HapII CCGG 1 cut(s) 40
HhaI GCGC 3 cut(s) 15, 245, 610
Hin1II CATG 4 cut(s) 147, 289, 509, 619
Hin6I GCGC 3 cut(s) 13, 243, 608
HinP1I GCGC 3 cut(s) 13, 243, 608
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HinfI GANTC 2 cut(s) 137, 496
HpaII CCGG 1 cut(s) 40
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 4 cut(s) 115, 117, 132, 141
Hpy188III TCNNGA 2 cut(s) 291, 520
Hpy8I GTNNAC 4 cut(s) 115, 117, 132, 141
HpyCH4III ACNGT 3 cut(s) 121, 324, 425
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 7 cut(s) 67, 174, 478, 505, 563, 570, 575
HpyF3I CTNAG 2 cut(s) 306, 404
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 4 cut(s) 147, 289, 509, 619
HspAI GCGC 3 cut(s) 13, 243, 608
KspI CCGCGG 1 cut(s) 8
LmnI GCTCC 3 cut(s) 240, 342, 582
Lsp1109I GCAGC 9 cut(s) 267, 370, 373, 376, 379, 419, 597, 619, 622
LweI GCATC 2 cut(s) 76, 305
MaeI CTAG 1 cut(s) 643
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 121
MboII GAAGA 3 cut(s) 47, 79, 431
MhlI GDGCHC 1 cut(s) 155
MluCI AATT 1 cut(s) 625
MluI ACGCGT 1 cut(s) 224
MlyI GAGTC 1 cut(s) 146
Mph1103I ATGCAT 1 cut(s) 507
MslI CAYNNNNRTG 1 cut(s) 148
MspA1I CMGCKG 1 cut(s) 7
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 2 cut(s) 41, 82
MvaI CCWGG 1 cut(s) 82
MvnI CGCG 4 cut(s) 7, 226, 292, 600
NciI CCSGG 1 cut(s) 41
NlaIII CATG 4 cut(s) 147, 289, 509, 619
NlaIV GGNNCC 1 cut(s) 79
NmuCI GTSAC 1 cut(s) 121
NruI TCGCGA 1 cut(s) 292
NsiI ATGCAT 1 cut(s) 507
NspI RCATGY 2 cut(s) 147, 619
NspV TTCGAA 1 cut(s) 33
PaqCI CACCTGC 1 cut(s) 189
PciI ACATGT 1 cut(s) 615
PfeI GAWTC 1 cut(s) 496
PflFI GACNNNGTC 1 cut(s) 161
PflMI CCANNNNNTGG 2 cut(s) 205, 346
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
PscI ACATGT 1 cut(s) 615
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 1 cut(s) 79
PsyI GACNNNGTC 1 cut(s) 161
RruI TCGCGA 1 cut(s) 292
RsaI GTAC 2 cut(s) 23, 116
RsaNI GTAC 2 cut(s) 22, 115
RseI CAYNNNNRTG 1 cut(s) 148
SacII CCGCGG 1 cut(s) 8
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 2 cut(s) 41, 82
SduI GDGCHC 1 cut(s) 155
SetI ASST 9 cut(s) 31, 86, 162, 183, 264, 269, 339, 383, 634
SfaNI GCATC 2 cut(s) 76, 305
Sfr303I CCGCGG 1 cut(s) 8
SfuI TTCGAA 1 cut(s) 33
SgrBI CCGCGG 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 148
Sse9I AATT 1 cut(s) 625
SsiI CCGC 5 cut(s) 5, 7, 58, 429, 598
SspMI CTAG 1 cut(s) 643
StyD4I CCNGG 2 cut(s) 39, 80
TaaI ACNGT 3 cut(s) 121, 324, 425
TaiI ACGT 1 cut(s) 162
TaqI TCGA 2 cut(s) 33, 219
TasI AATT 1 cut(s) 625
TatI WGTACW 1 cut(s) 114
TauI GCSGC 3 cut(s) 7, 432, 600
TfiI GAWTC 1 cut(s) 496
TscAI CASTG 4 cut(s) 306, 329, 480, 577
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 9 cut(s) 280, 358, 361, 364, 367, 432, 585, 610, 632
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 1 cut(s) 320
TspRI CASTG 4 cut(s) 306, 329, 480, 577
Tth111I GACNNNGTC 1 cut(s) 161
Van91I CCANNNNNTGG 2 cut(s) 205, 346
XapI RAATTY 1 cut(s) 625
XceI RCATGY 2 cut(s) 147, 619
XspI CTAG 1 cut(s) 643
Zsp2I ATGCAT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.