RLG00000010800

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004740
Physical Location & Seq
Forward (+)
4441712 .. 4442383
672 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010800

Sequence Viewer

Length: 672 bp
ATGCCGCGGCCTAGCCGCTACAAGCCCTTCAATGACCGCTTGGAGCGTGCCCTCAACCAGCACATTCGCCTCCTGCACCGCTCCGGTGCCACCTTCTTCATCCTCGGCGACACCGGGAATGTGTACACTGCTACCATAACCAGTCACCCTAAATGCAGCTGCCCTGACCCTGTAACACCATGCAAGCATTTGCTGTTTGTGTATCTCCAAGTGTTGGGTCTTTCAGCCGATGACAAGTCTCTCAGGGACGGCACGTTCTCGCAGTGGGAGGTGCAGTGCATGCTGGGGCGGGAAACGCTGCCTGAATCGCTGGCCGGGGAAAGCGTGCGGCAGTGGTTTCATCAGCTGTATGATTTTCAGGGCAAGCAGAGGCAGCAGGGGTCTTCTTCGTCGTCGAGGCCAAGAGTGGTGATTGAAGAGGGGACTTGCTGCCCTGTTTGTTTGGATGAGATGGGGAAGCAAGACAAGGTGGTGGCTTGTGGGACGTGTAGAAACCCGATACACGAGGAATGTTTCTTGAAGTGGAAGAGGAGCGCGAGGAAGAAGCCAGCTCATTGTGTGATGTGCAGGGCGAGATGGGGGTCGATCCAGCAGGAGCAGGAGAAGTACTTGAACTTGGCGGCTTACGCTAGTACTAGCGAGGAGGATGAGGACGAAGACGAGTATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000209 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004706 GO:0004709 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006508 GO:0006511 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007254 GO:0007256 GO:0007346 GO:0008150 GO:0008152 GO:0008289 GO:0008432 GO:0009056 GO:0009057 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030163 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031434 GO:0032147 GO:0032231 GO:0032232 GO:0032268 GO:0032270 GO:0032446 GO:0032872 GO:0032874 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043632 GO:0043900 GO:0043902 GO:0043903 GO:0043933 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0046328 GO:0046330 GO:0046625 GO:0046777 GO:0046782 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048583 GO:0048584 GO:0050434 GO:0050789 GO:0050790 GO:0050792 GO:0050794 GO:0050896 GO:0051019 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051259 GO:0051338 GO:0051347 GO:0051403 GO:0051493 GO:0051494 GO:0051603 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0110053 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902531 GO:1902533 GO:1902680 GO:1902903 GO:1902904 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SWIM zf-RING_2

Protein Analysis

224

Amino Acids

25.53

Weight (kDa)

6.66

Isoelectric Point (pI)

59.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 143 - 190 4.2e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AccB7I CCANNNNNTGG 1 cut(s) 214
AccBSI CCGCTC 1 cut(s) 81
AccII CGCG 2 cut(s) 7, 536
AciI CCGC 8 cut(s) 5, 7, 16, 37, 79, 289, 328, 620
AclWI GGATC 1 cut(s) 580
AcoI YGGCCR 1 cut(s) 312
AfaI GTAC 3 cut(s) 125, 608, 634
AfiI CCNNNNNNNGG 1 cut(s) 214
AflIII ACRYGT 1 cut(s) 485
AgsI TTSAA 4 cut(s) 31, 416, 520, 613
AjiI CACGTC 1 cut(s) 486
AloI GAACNNNNNNTCC 2 cut(s) 239, 271
AluBI AGCT 3 cut(s) 159, 346, 551
AluI AGCT 3 cut(s) 159, 346, 551
Alw26I GTCTC 1 cut(s) 243
AlwI GGATC 1 cut(s) 580
AoxI GGCC 3 cut(s) 8, 312, 398
ApeKI GCWGC 5 cut(s) 156, 159, 298, 373, 429
AspLEI GCGC 1 cut(s) 536
AsuC2I CCSGG 2 cut(s) 115, 316
AsuHPI GGTGA 2 cut(s) 137, 421
BaeGI GKGCMC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 86
BauI CACGAG 1 cut(s) 503
BbsI GAAGAC 2 cut(s) 375, 663
BbvI GCAGC 5 cut(s) 146, 168, 285, 385, 416
BccI CCATC 2 cut(s) 445, 570
BceAI ACGGC 1 cut(s) 265
BcnI CCSGG 2 cut(s) 115, 316
BcoDI GTCTC 1 cut(s) 243
BfaI CTAG 3 cut(s) 12, 630, 636
BmcAI AGTACT 2 cut(s) 608, 634
Bme1390I CCNGG 2 cut(s) 115, 316
BmgBI CACGTC 1 cut(s) 486
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 2 cut(s) 115, 316
BpiI GAAGAC 2 cut(s) 375, 663
BpuMI CCSGG 2 cut(s) 115, 316
BsaJI CCNNGG 3 cut(s) 5, 103, 315
BsaWI WCCGGW 1 cut(s) 83
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse1I ACTGG 1 cut(s) 141
BseDI CCNNGG 3 cut(s) 5, 103, 315
BseGI GGATG 3 cut(s) 99, 451, 652
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMII CTCAG 1 cut(s) 256
BseNI ACTGG 1 cut(s) 141
BseRI GAGGAG 2 cut(s) 544, 656
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 5 cut(s) 146, 168, 285, 385, 416
BseYI CCCAGC 1 cut(s) 283
BsgI GTGCAG 3 cut(s) 59, 293, 586
Bsh1236I CGCG 2 cut(s) 7, 536
BshFI GGCC 3 cut(s) 10, 314, 400
BshNI GGYRCC 1 cut(s) 86
BsiSI CCGG 3 cut(s) 84, 114, 315
BslFI GGGAC 3 cut(s) 260, 436, 496
BslI CCNNNNNNNGG 1 cut(s) 214
BsmAI GTCTC 1 cut(s) 243
BsmFI GGGAC 3 cut(s) 260, 436, 496
BsnI GGCC 3 cut(s) 10, 314, 400
Bsp1286I GDGCHC 1 cut(s) 52
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 1 cut(s) 585
BspACI CCGC 8 cut(s) 5, 7, 16, 37, 79, 289, 328, 620
BspANI GGCC 3 cut(s) 10, 314, 400
BspCNI CTCAG 1 cut(s) 255
BspFNI CGCG 2 cut(s) 7, 536
BspLI GGNNCC 1 cut(s) 88
BspPI GGATC 1 cut(s) 580
BspT107I GGYRCC 1 cut(s) 86
BsrBI CCGCTC 1 cut(s) 81
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 1 cut(s) 141
BssECI CCNNGG 3 cut(s) 5, 103, 315
BssMI GATC 1 cut(s) 585
BssSI CACGAG 1 cut(s) 503
Bst2BI CACGAG 1 cut(s) 503
Bst6I CTCTTC 2 cut(s) 411, 521
BstAPI GCANNNNNTGC 1 cut(s) 280
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 7 cut(s) 48, 185, 281, 312, 326, 365, 549
BstDEI CTNAG 1 cut(s) 242
BstDSI CCRYGG 1 cut(s) 5
BstF5I GGATG 3 cut(s) 99, 451, 652
BstFNI CGCG 2 cut(s) 7, 536
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 1 cut(s) 588
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 1 cut(s) 585
BstMWI GCNNNNNNNGC 5 cut(s) 280, 295, 307, 373, 626
BstNSI RCATGY 1 cut(s) 283
BstSCI CCNGG 2 cut(s) 113, 314
BstSLI GKGCMC 1 cut(s) 52
BstUI CGCG 2 cut(s) 7, 536
BstV1I GCAGC 5 cut(s) 146, 168, 285, 385, 416
BstV2I GAAGAC 2 cut(s) 375, 663
BsuRI GGCC 3 cut(s) 10, 314, 400
BtgI CCRYGG 1 cut(s) 5
BtrI CACGTC 1 cut(s) 486
BtsCI GGATG 3 cut(s) 99, 451, 652
BtsI GCAGTG 4 cut(s) 126, 269, 281, 338
BtsIMutI CAGTG 4 cut(s) 126, 269, 281, 338
Cac8I GCNNGC 7 cut(s) 48, 185, 281, 312, 326, 365, 549
CfoI GCGC 1 cut(s) 536
Cfr42I CCGCGG 1 cut(s) 8
Csp6I GTAC 3 cut(s) 124, 607, 633
CviAII CATG 2 cut(s) 180, 280
CviQI GTAC 3 cut(s) 124, 607, 633
DdeI CTNAG 1 cut(s) 242
DpnI GATC 1 cut(s) 587
DpnII GATC 1 cut(s) 585
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 2 cut(s) 411, 521
EarI CTCTTC 2 cut(s) 411, 521
FaeI CATG 2 cut(s) 183, 283
FaiI YATR 5 cut(s) 137, 181, 281, 351, 666
FaqI GGGAC 3 cut(s) 260, 436, 496
FatI CATG 2 cut(s) 179, 279
FauI CCCGC 1 cut(s) 282
FokI GGATG 3 cut(s) 86, 458, 659
FspBI CTAG 3 cut(s) 12, 630, 636
GlaI GCGC 1 cut(s) 535
GsaI CCCAGC 1 cut(s) 287
HaeIII GGCC 3 cut(s) 10, 314, 400
HapII CCGG 3 cut(s) 84, 114, 315
HhaI GCGC 1 cut(s) 536
Hin1II CATG 2 cut(s) 183, 283
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HinfI GANTC 1 cut(s) 305
HpaII CCGG 3 cut(s) 84, 114, 315
HphI GGTGA 2 cut(s) 137, 421
Hpy166II GTNNAC 2 cut(s) 124, 126
Hpy188III TCNNGA 1 cut(s) 517
Hpy8I GTNNAC 2 cut(s) 124, 126
Hpy99I CGWCG 2 cut(s) 394, 397
HpyAV CCTTC 2 cut(s) 37, 103
HpyCH4IV ACGT 2 cut(s) 254, 485
HpyCH4V TGCA 6 cut(s) 76, 156, 183, 274, 279, 567
HpyF10VI GCNNNNNNNGC 5 cut(s) 280, 295, 307, 373, 626
HpyF3I CTNAG 1 cut(s) 242
HpySE526I ACGT 2 cut(s) 254, 485
Hsp92II CATG 2 cut(s) 183, 283
HspAI GCGC 1 cut(s) 534
KspI CCGCGG 1 cut(s) 8
Kzo9I GATC 1 cut(s) 585
LmnI GCTCC 4 cut(s) 43, 86, 531, 595
Lsp1109I GCAGC 5 cut(s) 146, 168, 285, 385, 416
MaeI CTAG 3 cut(s) 12, 630, 636
MaeII ACGT 2 cut(s) 254, 485
MaeIII GTNAC 2 cut(s) 143, 172
MalI GATC 1 cut(s) 587
MbiI CCGCTC 1 cut(s) 81
MboI GATC 1 cut(s) 585
MboII GAAGA 7 cut(s) 88, 375, 378, 428, 538, 553, 668
MhlI GDGCHC 1 cut(s) 52
MspA1I CMGCKG 3 cut(s) 7, 159, 346
MspI CCGG 3 cut(s) 84, 114, 315
MspR9I CCNGG 2 cut(s) 115, 316
MvnI CGCG 2 cut(s) 7, 536
MwoI GCNNNNNNNGC 5 cut(s) 280, 295, 307, 373, 626
NciI CCSGG 2 cut(s) 115, 316
NdeII GATC 1 cut(s) 585
NlaIII CATG 2 cut(s) 183, 283
NlaIV GGNNCC 1 cut(s) 88
NmeAIII GCCGAG 1 cut(s) 84
NmuCI GTSAC 1 cut(s) 143
NspI RCATGY 1 cut(s) 283
PaeI GCATGC 1 cut(s) 283
PfeI GAWTC 1 cut(s) 305
PflMI CCANNNNNTGG 1 cut(s) 214
PspFI CCCAGC 1 cut(s) 283
PspN4I GGNNCC 1 cut(s) 88
PvuII CAGCTG 2 cut(s) 159, 346
RsaI GTAC 3 cut(s) 125, 608, 634
RsaNI GTAC 3 cut(s) 124, 607, 633
SacII CCGCGG 1 cut(s) 8
Sau3AI GATC 1 cut(s) 585
ScaI AGTACT 2 cut(s) 608, 634
ScrFI CCNGG 2 cut(s) 115, 316
SduI GDGCHC 1 cut(s) 52
SetI ASST 8 cut(s) 95, 161, 257, 273, 348, 471, 488, 553
Sfr303I CCGCGG 1 cut(s) 8
SgrBI CCGCGG 1 cut(s) 8
SphI GCATGC 1 cut(s) 283
SsiI CCGC 8 cut(s) 5, 7, 16, 37, 79, 289, 328, 620
SspMI CTAG 3 cut(s) 12, 630, 636
StyD4I CCNGG 2 cut(s) 113, 314
TaiI ACGT 2 cut(s) 257, 488
TaqI TCGA 2 cut(s) 395, 584
TatI WGTACW 3 cut(s) 123, 606, 632
TauI GCSGC 5 cut(s) 7, 10, 18, 331, 623
TfiI GAWTC 1 cut(s) 305
TscAI CASTG 4 cut(s) 133, 269, 281, 338
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 5 cut(s) 156, 159, 298, 373, 429
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 2 cut(s) 88, 329
TspRI CASTG 4 cut(s) 133, 269, 281, 338
Van91I CCANNNNNTGG 1 cut(s) 214
XceI RCATGY 1 cut(s) 283
XspI CTAG 3 cut(s) 12, 630, 636
ZrmI AGTACT 2 cut(s) 608, 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.