MD15G1092800.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004740
Physical Location & Seq
Forward (+)
6447855 .. 6448598
744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1092800.v1.1.491

Sequence Viewer

Length: 744 bp
ATGGAGTCCGTCGCCTCCAACTCCTCCCCACAAAACCACCGCCTGGCCCAGCGGCCGCGAACACGTATCATACCCACGCAGCCCTTCGCCGACCGCATTCTCCGAGCCCTGCACCACAACCTCCGACTCCTTCACCACCGCGAATCCAACTTTTCTGTGTTAGGTGCCACGGGGAATGTGTACACTGTCTCCTTATCCGCCACCCCTTCATGCACGTGCCCTGACCGTACAACCCCATGCAAGCACATCCTCTTCGTGTTCCTTCGAGTGTTGGGCCTCCCCCTCGATGACGCGTGTCTGAGGAGGAAGACGCTGCGGTCGTGCCAGGTCAGCCGCCTCTTAGGGCTGCCTATGCTGCCGGAATCACTGGCGGCTGTCAGCCTTCGAGAGAGGTTTCATCAGCTGTTTTTTCAGGAATCTTCGTCGACAACAAGGCCACGCCATGACCTGGAGATTGAAGAGGGGAGGAGCTGCCCTATTTGTTTGGAGGAGATGGCGGGAAGGGAGGAGAGAGTGGTGGCTTGCGGGGCGTGCAGAAACCCTATTCACGAAGAGTGTTTGATGAAGTGGAAGCGGAGCAGCGGGAGAAGGTCGGCGAATTGTGGGATTTGTAGGGCAAGGTTGAAGGATATTAATAGGATTATGGAACAGGATAAGTACTTGAATTTGGCTGCTTATTATGCCACTAATCATGATCAAGATGACATTGACAATGCTGATGAGGGTCTATATGGTAATCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000209 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004706 GO:0004709 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0006357 GO:0006464 GO:0006468 GO:0006508 GO:0006511 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007254 GO:0007256 GO:0007346 GO:0008150 GO:0008152 GO:0008289 GO:0008432 GO:0009056 GO:0009057 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030163 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0031434 GO:0032147 GO:0032231 GO:0032232 GO:0032268 GO:0032270 GO:0032446 GO:0032872 GO:0032874 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043506 GO:0043507 GO:0043549 GO:0043632 GO:0043900 GO:0043902 GO:0043903 GO:0043933 GO:0044085 GO:0044087 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0045944 GO:0046328 GO:0046330 GO:0046625 GO:0046777 GO:0046782 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048583 GO:0048584 GO:0050434 GO:0050789 GO:0050790 GO:0050792 GO:0050794 GO:0050896 GO:0051019 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051259 GO:0051338 GO:0051347 GO:0051403 GO:0051493 GO:0051494 GO:0051603 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0065009 GO:0070302 GO:0070304 GO:0070647 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0110053 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902531 GO:1902533 GO:1902680 GO:1902903 GO:1902904 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SWIM zf-RING_2

Protein Analysis

248

Amino Acids

28.1

Weight (kDa)

8.89

Isoelectric Point (pI)

70.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIM PF04434 61 - 90 2.8e-06 SWIM zinc finger
zf-RING_2 PF13639 157 - 205 1.6e-07 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 316
AccB1I GGYRCC 1 cut(s) 164
AccB7I CCANNNNNTGG 2 cut(s) 43, 448
AccI GTMKAC 1 cut(s) 425
AccII CGCG 3 cut(s) 58, 141, 293
AcoI YGGCCR 1 cut(s) 53
AcsI RAATTY 1 cut(s) 664
AcvI CACGTG 1 cut(s) 216
AfaI GTAC 3 cut(s) 182, 229, 659
AfiI CCNNNNNNNGG 2 cut(s) 43, 448
AflIII ACRYGT 2 cut(s) 62, 291
AgsI TTSAA 3 cut(s) 458, 625, 664
AjnI CCWGG 3 cut(s) 42, 324, 447
AluBI AGCT 2 cut(s) 403, 471
AluI AGCT 2 cut(s) 403, 471
Alw26I GTCTC 1 cut(s) 193
AoxI GGCC 4 cut(s) 45, 53, 274, 434
ApeKI GCWGC 7 cut(s) 79, 313, 346, 355, 471, 579, 671
ApoI RAATTY 1 cut(s) 664
AseI ATTAAT 1 cut(s) 633
AspS9I GGNCC 2 cut(s) 46, 274
AsuHPI GGTGA 1 cut(s) 125
BaeGI GKGCMC 1 cut(s) 221
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 109
BbrPI CACGTG 1 cut(s) 216
BbsI GAAGAC 1 cut(s) 314
BbvI GCAGC 7 cut(s) 91, 300, 333, 342, 458, 591, 658
BccI CCATC 1 cut(s) 487
BciT130I CCWGG 3 cut(s) 44, 326, 449
BclI TGATCA 1 cut(s) 694
BcoDI GTCTC 1 cut(s) 193
BmcAI AGTACT 1 cut(s) 659
Bme1390I CCNGG 3 cut(s) 44, 326, 449
BmgT120I GGNCC 2 cut(s) 46, 274
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 3 cut(s) 44, 326, 449
BoxI GACNNNNGTC 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 314
BpmI CTGGAG 1 cut(s) 470
BsaAI YACGTR 2 cut(s) 65, 216
BsaJI CCNNGG 1 cut(s) 168
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 448
Bse1I ACTGG 1 cut(s) 372
BseBI CCWGG 3 cut(s) 44, 326, 449
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 246
BseLI CCNNNNNNNGG 2 cut(s) 43, 448
BseMII CTCAG 1 cut(s) 290
BseNI ACTGG 1 cut(s) 372
BseRI GAGGAG 5 cut(s) 13, 316, 481, 503, 521
BseSI GKGCMC 1 cut(s) 221
BseX3I CGGCCG 1 cut(s) 53
BseXI GCAGC 7 cut(s) 91, 300, 333, 342, 458, 591, 658
BseYI CCCAGC 1 cut(s) 48
BsgI GTGCAG 2 cut(s) 95, 553
Bsh1236I CGCG 3 cut(s) 58, 141, 293
Bsh1285I CGRYCG 3 cut(s) 56, 94, 320
BshFI GGCC 4 cut(s) 47, 55, 276, 436
BshNI GGYRCC 1 cut(s) 164
BsiEI CGRYCG 3 cut(s) 56, 94, 320
BsiSI CCGG 1 cut(s) 359
BslI CCNNNNNNNGG 2 cut(s) 43, 448
BsmAI GTCTC 1 cut(s) 193
BsmI GAATGC 1 cut(s) 96
BsnI GGCC 4 cut(s) 47, 55, 276, 436
Bsp1286I GDGCHC 2 cut(s) 109, 221
Bsp1407I TGTACA 1 cut(s) 180
Bsp143I GATC 1 cut(s) 694
BspANI GGCC 4 cut(s) 47, 55, 276, 436
BspCNI CTCAG 1 cut(s) 291
BspFNI CGCG 3 cut(s) 58, 141, 293
BspHI TCATGA 1 cut(s) 691
BspLI GGNNCC 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 164
BsrGI TGTACA 1 cut(s) 180
BsrI ACTGG 1 cut(s) 372
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 1 cut(s) 694
Bst2UI CCWGG 3 cut(s) 44, 326, 449
Bst4CI ACNGT 2 cut(s) 187, 227
Bst6I CTCTTC 3 cut(s) 257, 453, 546
BstAUI TGTACA 1 cut(s) 180
BstBAI YACGTR 2 cut(s) 65, 216
BstC8I GCNNGC 3 cut(s) 242, 523, 532
BstDEI CTNAG 2 cut(s) 299, 340
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 1 cut(s) 246
BstFNI CGCG 3 cut(s) 58, 141, 293
BstKTI GATC 1 cut(s) 697
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 1 cut(s) 694
BstMCI CGRYCG 3 cut(s) 56, 94, 320
BstMWI GCNNNNNNNGC 6 cut(s) 330, 352, 355, 527, 531, 680
BstNI CCWGG 3 cut(s) 44, 326, 449
BstPAI GACNNNNGTC 1 cut(s) 294
BstSCI CCNGG 3 cut(s) 42, 324, 447
BstSLI GKGCMC 1 cut(s) 221
BstUI CGCG 3 cut(s) 58, 141, 293
BstV1I GCAGC 7 cut(s) 91, 300, 333, 342, 458, 591, 658
BstV2I GAAGAC 1 cut(s) 314
BstZI CGGCCG 1 cut(s) 53
BsuRI GGCC 4 cut(s) 47, 55, 276, 436
BtgI CCRYGG 1 cut(s) 168
BtsCI GGATG 1 cut(s) 246
BtsIMutI CAGTG 2 cut(s) 183, 365
Cac8I GCNNGC 3 cut(s) 242, 523, 532
CciI TCATGA 1 cut(s) 691
CciNI GCGGCCGC 1 cut(s) 53
Cfr13I GGNCC 2 cut(s) 46, 274
CseI GACGC 2 cut(s) 299, 319
Csp6I GTAC 3 cut(s) 181, 228, 658
CviAII CATG 4 cut(s) 210, 237, 443, 692
CviQI GTAC 3 cut(s) 181, 228, 658
DdeI CTNAG 2 cut(s) 299, 340
DpnI GATC 1 cut(s) 696
DpnII GATC 1 cut(s) 694
DrdI GACNNNNNNGTC 1 cut(s) 316
DseDI GACNNNNNNGTC 1 cut(s) 316
EaeI YGGCCR 1 cut(s) 53
EagI CGGCCG 1 cut(s) 53
Eam1104I CTCTTC 3 cut(s) 257, 453, 546
EarI CTCTTC 3 cut(s) 257, 453, 546
EciI GGCGGA 1 cut(s) 187
EclXI CGGCCG 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 109
Eco52I CGGCCG 1 cut(s) 53
Eco72I CACGTG 1 cut(s) 216
EcoRII CCWGG 3 cut(s) 42, 324, 447
EcoT38I GRGCYC 1 cut(s) 109
FaeI CATG 4 cut(s) 213, 240, 446, 695
FatI CATG 4 cut(s) 209, 236, 442, 691
FauI CCCGC 3 cut(s) 490, 518, 575
FbaI TGATCA 1 cut(s) 694
FblI GTMKAC 1 cut(s) 425
FokI GGATG 1 cut(s) 233
FriOI GRGCYC 1 cut(s) 109
GsaI CCCAGC 1 cut(s) 52
GsuI CTGGAG 1 cut(s) 470
HaeIII GGCC 4 cut(s) 47, 55, 276, 436
HapII CCGG 1 cut(s) 359
HgaI GACGC 2 cut(s) 299, 319
Hin1II CATG 4 cut(s) 213, 240, 446, 695
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
HinfI GANTC 5 cut(s) 5, 126, 143, 362, 416
HpaII CCGG 1 cut(s) 359
HphI GGTGA 1 cut(s) 125
Hpy166II GTNNAC 3 cut(s) 181, 183, 426
Hpy188I TCNGA 3 cut(s) 104, 125, 300
Hpy188III TCNNGA 5 cut(s) 386, 413, 548, 692, 698
Hpy8I GTNNAC 3 cut(s) 181, 183, 426
Hpy99I CGWCG 2 cut(s) 14, 427
HpyAV CCTTC 8 cut(s) 94, 140, 216, 272, 392, 495, 582, 619
HpyCH4III ACNGT 2 cut(s) 187, 227
HpyCH4IV ACGT 2 cut(s) 64, 215
HpyCH4V TGCA 4 cut(s) 112, 213, 240, 534
HpyF10VI GCNNNNNNNGC 6 cut(s) 330, 352, 355, 527, 531, 680
HpyF3I CTNAG 2 cut(s) 299, 340
HpySE526I ACGT 2 cut(s) 64, 215
Hsp92II CATG 4 cut(s) 213, 240, 446, 695
Ksp22I TGATCA 1 cut(s) 694
Kzo9I GATC 1 cut(s) 694
LmnI GCTCC 2 cut(s) 468, 576
Lsp1109I GCAGC 7 cut(s) 91, 300, 333, 342, 458, 591, 658
MaeII ACGT 2 cut(s) 64, 215
MalI GATC 1 cut(s) 696
MboI GATC 1 cut(s) 694
MboII GAAGA 5 cut(s) 244, 319, 411, 470, 563
MhlI GDGCHC 2 cut(s) 109, 221
MluCI AATT 2 cut(s) 598, 664
MluI ACGCGT 1 cut(s) 291
MlyI GAGTC 2 cut(s) 14, 120
MmeI TCCRAC 3 cut(s) 42, 148, 171
MseI TTAA 1 cut(s) 633
MslI CAYNNNNRTG 1 cut(s) 214
MspA1I CMGCKG 3 cut(s) 52, 403, 582
MspI CCGG 1 cut(s) 359
MspR9I CCNGG 3 cut(s) 44, 326, 449
Mva1269I GAATGC 1 cut(s) 96
MvaI CCWGG 3 cut(s) 44, 326, 449
MvnI CGCG 3 cut(s) 58, 141, 293
MwoI GCNNNNNNNGC 6 cut(s) 330, 352, 355, 527, 531, 680
NdeII GATC 1 cut(s) 694
NlaIII CATG 4 cut(s) 213, 240, 446, 695
NlaIV GGNNCC 1 cut(s) 166
NotI GCGGCCGC 1 cut(s) 53
PagI TCATGA 1 cut(s) 691
PcsI WCGNNNNNNNCGW 1 cut(s) 317
PctI GAATGC 1 cut(s) 96
PfeI GAWTC 3 cut(s) 143, 362, 416
PflMI CCANNNNNTGG 2 cut(s) 43, 448
PleI GAGTC 2 cut(s) 13, 120
PmaCI CACGTG 1 cut(s) 216
PmlI CACGTG 1 cut(s) 216
PpsI GAGTC 2 cut(s) 13, 120
Ppu21I YACGTR 2 cut(s) 65, 216
PshAI GACNNNNGTC 1 cut(s) 294
PshBI ATTAAT 1 cut(s) 633
Psp6I CCWGG 3 cut(s) 42, 324, 447
PspCI CACGTG 1 cut(s) 216
PspFI CCCAGC 1 cut(s) 48
PspGI CCWGG 3 cut(s) 42, 324, 447
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 2 cut(s) 46, 274
PvuII CAGCTG 1 cut(s) 403
RsaI GTAC 3 cut(s) 182, 229, 659
RsaNI GTAC 3 cut(s) 181, 228, 658
RseI CAYNNNNRTG 1 cut(s) 214
SalI GTCGAC 1 cut(s) 424
SaqAI TTAA 1 cut(s) 633
Sau3AI GATC 1 cut(s) 694
Sau96I GGNCC 2 cut(s) 46, 274
ScaI AGTACT 1 cut(s) 659
SchI GAGTC 2 cut(s) 14, 120
ScrFI CCNGG 3 cut(s) 44, 326, 449
SduI GDGCHC 2 cut(s) 109, 221
SmiMI CAYNNNNRTG 1 cut(s) 214
Sse9I AATT 2 cut(s) 598, 664
StyD4I CCNGG 3 cut(s) 42, 324, 447
TaaI ACNGT 2 cut(s) 187, 227
TaiI ACGT 2 cut(s) 67, 218
TaqI TCGA 4 cut(s) 265, 285, 385, 425
TasI AATT 2 cut(s) 598, 664
TatI WGTACW 2 cut(s) 180, 657
TauI GCSGC 4 cut(s) 55, 58, 336, 374
TfiI GAWTC 3 cut(s) 143, 362, 416
Tru1I TTAA 1 cut(s) 633
Tru9I TTAA 1 cut(s) 633
TscAI CASTG 2 cut(s) 190, 372
TseI GCWGC 7 cut(s) 79, 313, 346, 355, 471, 579, 671
TspDTI ATGAA 3 cut(s) 198, 386, 578
TspRI CASTG 2 cut(s) 190, 372
Van91I CCANNNNNTGG 2 cut(s) 43, 448
VspI ATTAAT 1 cut(s) 633
XapI RAATTY 1 cut(s) 664
XmiI GTMKAC 1 cut(s) 425
ZrmI AGTACT 1 cut(s) 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.