AT5G14390

Alpha beta hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006460
Physical Location & Seq
Reverse (-)
4637287 .. 4639835
2549 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G14390.1

Sequence Viewer

Length: 1110 bp
ATGGGAGGTGTGACGTCATCAGTTGCGGCGAAGTTCGCTTTCTTCCCGCCGAGTCCGTCGTCTTACAAGCTGGTTTACGATGAACTGACCGGACTTCTTCTGATGAACCCATTTCCTCACCGTGAAAACGTTGAAATCCTTAAGCTTCCGACACGTAGAGGAACAGAGATCGTGGCTATGTACGTCAGACATCCGATGGCTACCTCTACTCTGCTTTATTCCCATGGAAACGCCGCCGATCTGGGCCAGATGTATGAGCTCTTCATCGAATTGAGCATCCATCTTAAGGTTAATCTCATGGGGTACGATTACTCCGGGTATGGACAATCTACTGGAAAGCCAAGTGAGCACCATACATATGCTGATATCGAAGCTGCTTATAAATGTCTTGAGGAAACCTATGGAGCAAAACAGGAAGACATAATCCTTTATGGCCAATCCGTTGGAAGTGGACCTACATTAGATCTTGCTGCTCGGTTGCCTCAATTAAGAGCTGCTGTTCTCCATAGCCCGATTCTTTCTGGTCTAAGAGTAATGTATCCAGTGAAGAAGACATACTGGTTCGACATCTTCAAGAATATCGACAAAATACCTCTTGTGAATTGCCCGGTTCTCGTCATTCACGGAACTTGTGATGAAGTTGTTGACTGTTCTCATGGAAAACAACTATGGGAACTCTCTAAAGAGAAATATGAACCGCTTTGGCTCGAAGGCGGTAACCACTGTGATCTAGAACACTATCCTGAATACATTAAACACCTTAAGAAGTTCATCACAACCGTAGAGAGAGATCTCTCCTCGAGGGCGAGCACGGCCCAATTAGAGAAACAGAGCAGCGATTTGGAAATGCCGAGGCAGAGCGTAGATAGAAGAGAGAAGCCGAGGCAAAGCGTTGATAAAAGGGAGAAAGAGAAACCTCCAAAGGGTCCTTCAAAGAAGAGTAAGCTGAGAATCACGTTCGAGCAGCACTTAGATCGGACTAGGAGAAGCGTTGACTTCCACGAAAAGGCAAGGAAGAGCGTTGACCATCATCAGCATCATCAGAATCATCATCAGATTGAGAGAGGAAGGAAGAGTGTTGATAGATTGGATAGAGTACGGTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

369

Amino Acids

42.34

Weight (kDa)

8.72

Isoelectric Point (pI)

51.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 70 - 175 1.5e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 71 - 171 4.5e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 381
AatII GACGTC 1 cut(s) 17
AbsI CCTCGAGG 1 cut(s) 799
AciI CCGC 5 cut(s) 26, 47, 234, 698, 714
AclI AACGTT 1 cut(s) 129
AcoI YGGCCR 1 cut(s) 433
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 3 cut(s) 182, 305, 1098
AfiI CCNNNNNNNGG 3 cut(s) 286, 923, 1006
AflII CTTAAG 3 cut(s) 140, 284, 761
AflIII ACRYGT 1 cut(s) 152
AgsI TTSAA 3 cut(s) 134, 574, 933
AjuI GAANNNNNNNTTGG 2 cut(s) 913, 945
AluBI AGCT 6 cut(s) 70, 145, 259, 374, 494, 946
AluI AGCT 6 cut(s) 70, 145, 259, 374, 494, 946
Alw21I GWGCWC 3 cut(s) 261, 351, 812
Ama87I CYCGRG 1 cut(s) 799
AoxI GGCC 3 cut(s) 244, 433, 813
ApeKI GCWGC 5 cut(s) 374, 470, 494, 834, 964
AspS9I GGNCC 4 cut(s) 244, 452, 814, 926
AsuC2I CCSGG 2 cut(s) 316, 608
AsuHPI GGTGA 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 799
AvaII GGWCC 2 cut(s) 452, 926
BalI TGGCCA 1 cut(s) 435
BanII GRGCYC 1 cut(s) 261
BarI GAAGNNNNNNTAC 6 cut(s) 245, 277, 439, 471, 539, 571
BbsI GAAGAC 2 cut(s) 423, 557
Bbv12I GWGCWC 3 cut(s) 261, 351, 812
BbvI GCAGC 5 cut(s) 361, 457, 481, 846, 976
BccI CCATC 3 cut(s) 190, 288, 1035
BceAI ACGGC 1 cut(s) 828
BcgI CGANNNNNNTGC 2 cut(s) 956, 990
BciVI GTATCC 1 cut(s) 549
BcnI CCSGG 2 cut(s) 316, 608
BfaI CTAG 2 cut(s) 731, 981
BfrI CTTAAG 3 cut(s) 140, 284, 761
BfuI GTATCC 1 cut(s) 549
BglII AGATCT 2 cut(s) 463, 790
BisI GCNGC 7 cut(s) 27, 234, 375, 471, 495, 835, 965
BlsI GCNGC 7 cut(s) 28, 235, 376, 472, 496, 836, 966
Bme1390I CCNGG 2 cut(s) 316, 608
Bme18I GGWCC 2 cut(s) 452, 926
BmeT110I CYCGRG 1 cut(s) 799
BmgT120I GGNCC 4 cut(s) 244, 452, 814, 926
BmiI GGNNCC 1 cut(s) 927
BmrFI CCNGG 2 cut(s) 316, 608
BmsI GCATC 2 cut(s) 285, 1045
BpiI GAAGAC 2 cut(s) 423, 557
BpuEI CTTGAG 1 cut(s) 410
BpuMI CCSGG 2 cut(s) 316, 608
BsaAI YACGTR 1 cut(s) 155
BsaHI GRCGYC 1 cut(s) 14
BsaJI CCNNGG 3 cut(s) 223, 851, 881
BsaWI WCCGGW 1 cut(s) 89
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 923, 1006
Bse1I ACTGG 3 cut(s) 337, 542, 563
BseDI CCNNGG 3 cut(s) 223, 851, 881
BseGI GGATG 2 cut(s) 190, 276
BseLI CCNNNNNNNGG 3 cut(s) 286, 923, 1006
BseMII CTCAG 2 cut(s) 938, 1095
BseNI ACTGG 3 cut(s) 337, 542, 563
BseRI GAGGAG 1 cut(s) 787
BseXI GCAGC 5 cut(s) 361, 457, 481, 846, 976
BshFI GGCC 3 cut(s) 246, 435, 815
BsiHKAI GWGCWC 3 cut(s) 261, 351, 812
BsiHKCI CYCGRG 1 cut(s) 799
BsiSI CCGG 3 cut(s) 90, 315, 608
BslI CCNNNNNNNGG 3 cut(s) 286, 923, 1006
BsnI GGCC 3 cut(s) 246, 435, 815
BsoBI CYCGRG 1 cut(s) 799
Bsp1286I GDGCHC 3 cut(s) 261, 351, 812
Bsp143I GATC 6 cut(s) 168, 238, 463, 727, 790, 973
Bsp19I CCATGG 1 cut(s) 223
BspACI CCGC 5 cut(s) 26, 47, 234, 698, 714
BspANI GGCC 3 cut(s) 246, 435, 815
BspCNI CTCAG 2 cut(s) 939, 1096
BspLI GGNNCC 1 cut(s) 927
BspQI GCTCTTC 2 cut(s) 266, 1010
BspTI CTTAAG 3 cut(s) 140, 284, 761
BsrI ACTGG 3 cut(s) 337, 542, 563
BssECI CCNNGG 3 cut(s) 223, 851, 881
BssMI GATC 6 cut(s) 168, 238, 463, 727, 790, 973
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 223
Bst4CI ACNGT 5 cut(s) 122, 650, 725, 781, 1101
Bst6I CTCTTC 5 cut(s) 266, 865, 932, 1010, 1067
BstACI GRCGYC 1 cut(s) 14
BstAFI CTTAAG 3 cut(s) 140, 284, 761
BstBAI YACGTR 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 808
BstDEI CTNAG 4 cut(s) 527, 947, 970, 1104
BstDSI CCRYGG 1 cut(s) 223
BstEII GGTNACC 1 cut(s) 716
BstF5I GGATG 2 cut(s) 190, 276
BstKTI GATC 6 cut(s) 171, 241, 466, 730, 793, 976
BstMBI GATC 6 cut(s) 168, 238, 463, 727, 790, 973
BstMWI GCNNNNNNNGC 3 cut(s) 35, 346, 812
BstPI GGTNACC 1 cut(s) 716
BstSCI CCNGG 2 cut(s) 314, 606
BstV1I GCAGC 5 cut(s) 361, 457, 481, 846, 976
BstV2I GAAGAC 2 cut(s) 423, 557
BstX2I RGATCY 2 cut(s) 463, 790
BstXI CCANNNNNNTGG 1 cut(s) 443
BstYI RGATCY 2 cut(s) 463, 790
BsuI GTATCC 1 cut(s) 549
BsuRI GGCC 3 cut(s) 246, 435, 815
BtgI CCRYGG 1 cut(s) 223
BtsCI GGATG 2 cut(s) 190, 276
BtsIMutI CAGTG 2 cut(s) 549, 721
Cac8I GCNNGC 1 cut(s) 808
Cfr13I GGNCC 4 cut(s) 244, 452, 814, 926
Csp6I GTAC 3 cut(s) 181, 304, 1097
CviAII CATG 3 cut(s) 224, 298, 656
CviQI GTAC 3 cut(s) 181, 304, 1097
DdeI CTNAG 4 cut(s) 527, 947, 970, 1104
DpnI GATC 6 cut(s) 170, 240, 465, 729, 792, 975
DpnII GATC 6 cut(s) 168, 238, 463, 727, 790, 973
EaeI YGGCCR 1 cut(s) 433
Eam1104I CTCTTC 5 cut(s) 266, 865, 932, 1010, 1067
EarI CTCTTC 5 cut(s) 266, 865, 932, 1010, 1067
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 261
Eco32I GATATC 1 cut(s) 367
Eco47I GGWCC 2 cut(s) 452, 926
Eco53kI GAGCTC 1 cut(s) 259
Eco88I CYCGRG 1 cut(s) 799
Eco91I GGTNACC 1 cut(s) 716
EcoICRI GAGCTC 1 cut(s) 259
EcoO109I RGGNCCY 1 cut(s) 926
EcoO65I GGTNACC 1 cut(s) 716
EcoRV GATATC 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 223
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 223
FaeI CATG 3 cut(s) 227, 301, 659
FatI CATG 3 cut(s) 223, 297, 655
FauI CCCGC 1 cut(s) 54
FauNDI CATATG 1 cut(s) 358
Fnu4HI GCNGC 7 cut(s) 27, 234, 375, 471, 495, 835, 965
FokI GGATG 2 cut(s) 177, 263
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 7 cut(s) 27, 234, 375, 471, 495, 835, 965
FspBI CTAG 2 cut(s) 731, 981
GluI GCNGC 7 cut(s) 27, 234, 375, 471, 495, 835, 965
HaeIII GGCC 3 cut(s) 246, 435, 815
HapII CCGG 3 cut(s) 90, 315, 608
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 3 cut(s) 227, 301, 659
HincII GTYRAC 3 cut(s) 646, 994, 1024
HindII GTYRAC 3 cut(s) 646, 994, 1024
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 4 cut(s) 52, 514, 951, 1045
HpaII CCGG 3 cut(s) 90, 315, 608
HphI GGTGA 1 cut(s) 110
Hpy166II GTNNAC 5 cut(s) 76, 452, 646, 994, 1024
Hpy188I TCNGA 8 cut(s) 102, 150, 188, 195, 978, 1044, 1056, 1105
Hpy188III TCNNGA 4 cut(s) 389, 574, 731, 743
Hpy8I GTNNAC 5 cut(s) 76, 452, 646, 994, 1024
Hpy99I CGWCG 1 cut(s) 61
HpyAV CCTTC 3 cut(s) 704, 939, 1062
HpyCH4III ACNGT 5 cut(s) 122, 650, 725, 781, 1101
HpyCH4IV ACGT 5 cut(s) 14, 129, 154, 183, 956
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 346, 812
HpyF3I CTNAG 4 cut(s) 527, 947, 970, 1104
HpySE526I ACGT 5 cut(s) 14, 129, 154, 183, 956
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 3 cut(s) 227, 301, 659
Kzo9I GATC 6 cut(s) 168, 238, 463, 727, 790, 973
LguI GCTCTTC 2 cut(s) 266, 1010
LmnI GCTCC 1 cut(s) 404
Lsp1109I GCAGC 5 cut(s) 361, 457, 481, 846, 976
LweI GCATC 2 cut(s) 285, 1045
MaeI CTAG 2 cut(s) 731, 981
MaeII ACGT 5 cut(s) 14, 129, 154, 183, 956
MaeIII GTNAC 2 cut(s) 10, 716
MalI GATC 6 cut(s) 170, 240, 465, 729, 792, 975
MboI GATC 6 cut(s) 168, 238, 463, 727, 790, 973
MflI RGATCY 2 cut(s) 463, 790
MhlI GDGCHC 3 cut(s) 261, 351, 812
MlsI TGGCCA 1 cut(s) 435
MluCI AATT 4 cut(s) 269, 485, 601, 818
MluNI TGGCCA 1 cut(s) 435
MlyI GAGTC 1 cut(s) 61
MmeI TCCRAC 2 cut(s) 173, 424
Mox20I TGGCCA 1 cut(s) 435
MscI TGGCCA 1 cut(s) 435
MseI TTAA 6 cut(s) 141, 285, 291, 488, 753, 762
MslI CAYNNNNRTG 1 cut(s) 357
Msp20I TGGCCA 1 cut(s) 435
MspCI CTTAAG 3 cut(s) 140, 284, 761
MspI CCGG 3 cut(s) 90, 315, 608
MspR9I CCNGG 2 cut(s) 316, 608
MwoI GCNNNNNNNGC 3 cut(s) 35, 346, 812
NciI CCSGG 2 cut(s) 316, 608
NcoI CCATGG 1 cut(s) 223
NdeI CATATG 1 cut(s) 358
NdeII GATC 6 cut(s) 168, 238, 463, 727, 790, 973
NlaIII CATG 3 cut(s) 227, 301, 659
NlaIV GGNNCC 1 cut(s) 927
NmeAIII GCCGAG 3 cut(s) 75, 876, 906
NmuCI GTSAC 1 cut(s) 10
PaeR7I CTCGAG 1 cut(s) 799
PciSI GCTCTTC 2 cut(s) 266, 1010
PfeI GAWTC 3 cut(s) 514, 951, 1045
PkrI GCNGC 7 cut(s) 28, 235, 376, 472, 496, 836, 966
PleI GAGTC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 60
Ppu21I YACGTR 1 cut(s) 155
PpuMI RGGWCCY 1 cut(s) 926
PsiI TTATAA 1 cut(s) 381
Psp124BI GAGCTC 1 cut(s) 261
Psp1406I AACGTT 1 cut(s) 129
Psp5II RGGWCCY 1 cut(s) 926
PspEI GGTNACC 1 cut(s) 716
PspN4I GGNNCC 1 cut(s) 927
PspPI GGNCC 4 cut(s) 244, 452, 814, 926
PspPPI RGGWCCY 1 cut(s) 926
PspXI VCTCGAGB 1 cut(s) 799
PsuI RGATCY 2 cut(s) 463, 790
RsaI GTAC 3 cut(s) 182, 305, 1098
RsaNI GTAC 3 cut(s) 181, 304, 1097
RseI CAYNNNNRTG 1 cut(s) 357
SacI GAGCTC 1 cut(s) 261
SapI GCTCTTC 2 cut(s) 266, 1010
SaqAI TTAA 6 cut(s) 141, 285, 291, 488, 753, 762
SatI GCNGC 7 cut(s) 27, 234, 375, 471, 495, 835, 965
Sau3AI GATC 6 cut(s) 168, 238, 463, 727, 790, 973
Sau96I GGNCC 4 cut(s) 244, 452, 814, 926
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 2 cut(s) 316, 608
SduI GDGCHC 3 cut(s) 261, 351, 812
SfaNI GCATC 2 cut(s) 285, 1045
Sfr274I CTCGAG 1 cut(s) 799
SinI GGWCC 2 cut(s) 452, 926
SlaI CTCGAG 1 cut(s) 799
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 5 cut(s) 140, 284, 389, 761, 799
SmoI CTYRAG 5 cut(s) 140, 284, 389, 761, 799
Sse9I AATT 4 cut(s) 269, 485, 601, 818
SsiI CCGC 5 cut(s) 26, 47, 234, 698, 714
SspMI CTAG 2 cut(s) 731, 981
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 2 cut(s) 314, 606
StyI CCWWGG 1 cut(s) 223
TaaI ACNGT 5 cut(s) 122, 650, 725, 781, 1101
TaiI ACGT 5 cut(s) 17, 132, 157, 186, 959
TaqI TCGA 7 cut(s) 267, 369, 564, 582, 708, 800, 960
TasI AATT 4 cut(s) 269, 485, 601, 818
TauI GCSGC 2 cut(s) 29, 236
TfiI GAWTC 3 cut(s) 514, 951, 1045
Tru1I TTAA 6 cut(s) 141, 285, 291, 488, 753, 762
Tru9I TTAA 6 cut(s) 141, 285, 291, 488, 753, 762
TscAI CASTG 2 cut(s) 549, 728
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 5 cut(s) 374, 470, 494, 834, 964
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 6 cut(s) 96, 119, 253, 651, 708, 760
TspGWI ACGGA 3 cut(s) 45, 430, 639
TspRI CASTG 2 cut(s) 549, 728
Vha464I CTTAAG 3 cut(s) 140, 284, 761
VpaK11BI GGWCC 2 cut(s) 452, 926
XbaI TCTAGA 1 cut(s) 730
XhoI CTCGAG 1 cut(s) 799
XspI CTAG 2 cut(s) 731, 981
ZraI GACGTC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.