RLG00000022205

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006460
Physical Location & Seq
Forward (+)
81693229 .. 81696131
2903 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022205

Sequence Viewer

Length: 1086 bp
ATGGCGGCCAAGTTCGCCTTTTTCCCGCCGAACCCGCCGTCGTACAAGCTCATCACCGACGAGCTGACGGGGCTGTTGCTGCTGAGCCCATTTCCGCACAGGGAAAACGTGGAGGTTCTGAAGCTGCCGACTCGGCGCGGCACTGAGGTGGTGGCTATTTACATTAGGCACCCTTTGGCCACTTCTACTCTGCTTTACTCTCACGGTAACGCCGCCGATCTGGGCCAGATGTATGAGCTCTTCATCGAGCTCAGCATCCATTTGAGGGTTAATCTAATGGGGTATGACTATTCAGGGTATGGGCAATCATCTGGAAAGGCAAGTGAGCAGAATACATACGCAGATATTGAAGCTGCATATAAGTGTCTTGAAGAAAGCTATGGTACTAAGCAGGAAGATATCATTCTCTATGGTCAATCTGTTGGAAGTGGCCCGACTTTGGATCTTGCTGCTCGGCTGCCTCAGTTAAGAGCTGTTGTCCTGCACAGTCCCATCCTATCTGGTTTAAGAGTCATGTATCCTGTAAAGCGTACATACTGGTTTGACATTTATAAGAATATTGATAAGATTCCACTGGTCAATTGTCCTGTTCTCATCATTCATGGAACTGCAGATGAGGTAGTTGACTGCTCTCATGGCAAGCAACTGTGGGAATTATGTAAAGAGAAGTATGAACCACTATGGCTTAAAGGAGGCAACCACTGTGATATAGAACTGTATCCCGAGTACATCAGGCATCTCAAGAAGTTCATTTCAACTGTCGAGAAGTCTCCTTCCCAAAGATACAGTTCTAGGAGAAGCACAGACCAGTTTGAGCAGCCTCGGAAGAGTACTGATGTTTACGAAGTTTCTAGGAAGAGCACTGATCGAAGAGATAAACCAAAGAAGAGCACTGATAGGCCAGAGAAACTGAAAAACCAGTTCATTAGTGCTGATAAACTAGAAAAATTAAAAGTTTCCTTTGACCATATGGACAGGTCTCGGAGAAGCGTGGACTGCCATGAGAAGTCCAGAAAAAGTGTTGACCACCAACTGGATAGAGCACGGAAGAGTGTGGATCGATTGGATAGAATACGGAGTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

362

Amino Acids

41.5

Weight (kDa)

9.05

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 64 - 168 3.6e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 64 - 164 1.3e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AccB1I GGYRCC 1 cut(s) 168
AccB7I CCANNNNNTGG 1 cut(s) 1033
AccII CGCG 1 cut(s) 138
AciI CCGC 6 cut(s) 5, 26, 35, 95, 138, 213
AclWI GGATC 2 cut(s) 450, 1065
AcoI YGGCCR 2 cut(s) 6, 177
AcuI CTGAAG 1 cut(s) 140
AfaI GTAC 5 cut(s) 44, 385, 532, 728, 832
AfiI CCNNNNNNNGG 3 cut(s) 265, 439, 1033
AgsI TTSAA 3 cut(s) 350, 371, 756
AleI CACNNNNGTG 1 cut(s) 146
AluBI AGCT 8 cut(s) 49, 64, 124, 238, 250, 353, 378, 473
AluI AGCT 8 cut(s) 49, 64, 124, 238, 250, 353, 378, 473
Alw21I GWGCWC 5 cut(s) 240, 252, 863, 893, 1045
Alw26I GTCTC 2 cut(s) 774, 984
AlwI GGATC 2 cut(s) 450, 1065
Ama87I CYCGRG 1 cut(s) 722
AoxI GGCC 5 cut(s) 6, 177, 223, 430, 899
ApeKI GCWGC 6 cut(s) 79, 124, 353, 449, 457, 817
ArsI GACNNNNNNTTYG 2 cut(s) 23, 55
AspLEI GCGC 1 cut(s) 138
AspS9I GGNCC 2 cut(s) 223, 431
AsuHPI GGTGA 1 cut(s) 46
AvaI CYCGRG 1 cut(s) 722
BalI TGGCCA 1 cut(s) 179
BanI GGYRCC 1 cut(s) 168
BanII GRGCYC 3 cut(s) 89, 240, 252
BarI GAAGNNNNNNTAC 2 cut(s) 224, 256
Bbv12I GWGCWC 5 cut(s) 240, 252, 863, 893, 1045
BbvI GCAGC 6 cut(s) 66, 111, 340, 436, 444, 829
BccI CCATC 1 cut(s) 500
BceAI ACGGC 1 cut(s) 22
BciVI GTATCC 2 cut(s) 528, 729
BcoDI GTCTC 2 cut(s) 774, 984
BfaI CTAG 3 cut(s) 792, 852, 941
BfmI CTRYAG 1 cut(s) 609
BfuI GTATCC 2 cut(s) 528, 729
BglI GCCNNNNNGGC 1 cut(s) 133
BisI GCNGC 9 cut(s) 6, 80, 125, 139, 213, 354, 450, 458, 818
BlpI GCTNAGC 2 cut(s) 83, 251
BlsI GCNGC 9 cut(s) 7, 81, 126, 140, 214, 355, 451, 459, 819
BmcAI AGTACT 1 cut(s) 832
BmeT110I CYCGRG 1 cut(s) 722
BmgT120I GGNCC 2 cut(s) 223, 431
BmiI GGNNCC 1 cut(s) 170
BmsI GCATC 2 cut(s) 264, 745
Bpu1102I GCTNAGC 2 cut(s) 83, 251
BpuEI CTTGAG 1 cut(s) 725
Bsa29I ATCGAT 1 cut(s) 1060
BsaI GGTCTC 1 cut(s) 984
BsaJI CCNNGG 1 cut(s) 821
Bsc4I CCNNNNNNNGG 3 cut(s) 265, 439, 1033
Bse1I ACTGG 5 cut(s) 542, 579, 808, 919, 1038
BseCI ATCGAT 1 cut(s) 1060
BseDI CCNNGG 1 cut(s) 821
BseGI GGATG 2 cut(s) 255, 492
BseLI CCNNNNNNNGG 3 cut(s) 265, 439, 1033
BseMII CTCAG 4 cut(s) 74, 135, 265, 476
BseNI ACTGG 5 cut(s) 542, 579, 808, 919, 1038
BseXI GCAGC 6 cut(s) 66, 111, 340, 436, 444, 829
BsgI GTGCAG 1 cut(s) 467
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 5 cut(s) 8, 179, 225, 432, 901
BshNI GGYRCC 1 cut(s) 168
BshVI ATCGAT 1 cut(s) 1060
BsiHKAI GWGCWC 5 cut(s) 240, 252, 863, 893, 1045
BsiHKCI CYCGRG 1 cut(s) 722
BslFI GGGAC 1 cut(s) 474
BslI CCNNNNNNNGG 3 cut(s) 265, 439, 1033
BsmAI GTCTC 2 cut(s) 774, 984
BsmFI GGGAC 1 cut(s) 474
BsnI GGCC 5 cut(s) 8, 179, 225, 432, 901
Bso31I GGTCTC 1 cut(s) 984
BsoBI CYCGRG 1 cut(s) 722
Bsp1286I GDGCHC 6 cut(s) 89, 240, 252, 863, 893, 1045
Bsp143I GATC 4 cut(s) 217, 442, 865, 1057
Bsp1720I GCTNAGC 2 cut(s) 83, 251
BspACI CCGC 6 cut(s) 5, 26, 35, 95, 138, 213
BspANI GGCC 5 cut(s) 8, 179, 225, 432, 901
BspCNI CTCAG 4 cut(s) 75, 136, 264, 475
BspDI ATCGAT 1 cut(s) 1060
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 170
BspMAI CTGCAG 1 cut(s) 613
BspPI GGATC 2 cut(s) 450, 1065
BspQI GCTCTTC 3 cut(s) 245, 851, 881
BspT107I GGYRCC 1 cut(s) 168
BspTNI GGTCTC 1 cut(s) 984
BsrI ACTGG 5 cut(s) 542, 579, 808, 919, 1038
BssECI CCNNGG 1 cut(s) 821
BssMI GATC 4 cut(s) 217, 442, 865, 1057
Bst4CI ACNGT 7 cut(s) 206, 488, 648, 704, 717, 760, 788
Bst6I CTCTTC 6 cut(s) 245, 821, 851, 865, 881, 1043
BstC8I GCNNGC 1 cut(s) 641
BstDEI CTNAG 5 cut(s) 83, 144, 251, 387, 462
BstF5I GGATG 2 cut(s) 255, 492
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 1 cut(s) 138
BstKTI GATC 4 cut(s) 220, 445, 868, 1060
BstMAI GTCTC 2 cut(s) 774, 984
BstMBI GATC 4 cut(s) 217, 442, 865, 1057
BstMWI GCNNNNNNNGC 7 cut(s) 14, 34, 70, 79, 133, 636, 996
BstSFI CTRYAG 1 cut(s) 609
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 6 cut(s) 66, 111, 340, 436, 444, 829
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
Bsu15I ATCGAT 1 cut(s) 1060
BsuI GTATCC 2 cut(s) 528, 729
BsuRI GGCC 5 cut(s) 8, 179, 225, 432, 901
BsuTUI ATCGAT 1 cut(s) 1060
BtsCI GGATG 2 cut(s) 255, 492
BtsIMutI CAGTG 5 cut(s) 141, 572, 700, 861, 891
Cac8I GCNNGC 1 cut(s) 641
CfoI GCGC 1 cut(s) 138
Cfr13I GGNCC 2 cut(s) 223, 431
ClaI ATCGAT 1 cut(s) 1060
Csp6I GTAC 5 cut(s) 43, 384, 531, 727, 831
CviAII CATG 4 cut(s) 514, 602, 635, 1001
CviQI GTAC 5 cut(s) 43, 384, 531, 727, 831
DdeI CTNAG 5 cut(s) 83, 144, 251, 387, 462
DpnI GATC 4 cut(s) 219, 444, 867, 1059
DpnII GATC 4 cut(s) 217, 442, 865, 1057
EaeI YGGCCR 2 cut(s) 6, 177
Eam1104I CTCTTC 6 cut(s) 245, 821, 851, 865, 881, 1043
EarI CTCTTC 6 cut(s) 245, 821, 851, 865, 881, 1043
Ecl136II GAGCTC 2 cut(s) 238, 250
Eco24I GRGCYC 3 cut(s) 89, 240, 252
Eco31I GGTCTC 1 cut(s) 984
Eco32I GATATC 1 cut(s) 400
Eco53kI GAGCTC 2 cut(s) 238, 250
Eco57I CTGAAG 1 cut(s) 140
Eco88I CYCGRG 1 cut(s) 722
EcoICRI GAGCTC 2 cut(s) 238, 250
EcoRV GATATC 1 cut(s) 400
EcoT38I GRGCYC 3 cut(s) 89, 240, 252
FaeI CATG 4 cut(s) 517, 605, 638, 1004
FalI AAGNNNNNCTT 1 cut(s) 34
FaqI GGGAC 1 cut(s) 474
FatI CATG 4 cut(s) 513, 601, 634, 1000
FauI CCCGC 2 cut(s) 33, 42
FauNDI CATATG 1 cut(s) 969
Fnu4HI GCNGC 9 cut(s) 6, 80, 125, 139, 213, 354, 450, 458, 818
FokI GGATG 2 cut(s) 242, 479
FriOI GRGCYC 3 cut(s) 89, 240, 252
Fsp4HI GCNGC 9 cut(s) 6, 80, 125, 139, 213, 354, 450, 458, 818
FspBI CTAG 3 cut(s) 792, 852, 941
GlaI GCGC 1 cut(s) 137
GluI GCNGC 9 cut(s) 6, 80, 125, 139, 213, 354, 450, 458, 818
HaeIII GGCC 5 cut(s) 8, 179, 225, 432, 901
HhaI GCGC 1 cut(s) 138
Hin1II CATG 4 cut(s) 517, 605, 638, 1004
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HincII GTYRAC 2 cut(s) 625, 1024
HindII GTYRAC 2 cut(s) 625, 1024
HinfI GANTC 3 cut(s) 130, 510, 568
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 4 cut(s) 625, 841, 994, 1024
Hpy188I TCNGA 3 cut(s) 120, 825, 984
Hpy188III TCNNGA 6 cut(s) 312, 368, 722, 742, 763, 1011
Hpy8I GTNNAC 4 cut(s) 625, 841, 994, 1024
Hpy99I CGWCG 2 cut(s) 43, 62
HpyAV CCTTC 1 cut(s) 783
HpyCH4III ACNGT 7 cut(s) 206, 488, 648, 704, 717, 760, 788
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 3 cut(s) 356, 484, 611
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 34, 70, 79, 133, 636, 996
HpyF3I CTNAG 5 cut(s) 83, 144, 251, 387, 462
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 4 cut(s) 517, 605, 638, 1004
HspAI GCGC 1 cut(s) 136
Kzo9I GATC 4 cut(s) 217, 442, 865, 1057
LguI GCTCTTC 3 cut(s) 245, 851, 881
Lsp1109I GCAGC 6 cut(s) 66, 111, 340, 436, 444, 829
LweI GCATC 2 cut(s) 264, 745
MaeI CTAG 3 cut(s) 792, 852, 941
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 206
MalI GATC 4 cut(s) 219, 444, 867, 1059
MboI GATC 4 cut(s) 217, 442, 865, 1057
MboII GAAGA 8 cut(s) 232, 383, 407, 838, 868, 882, 898, 1060
MfeI CAATTG 1 cut(s) 580
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 6 cut(s) 89, 240, 252, 863, 893, 1045
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 3 cut(s) 580, 653, 947
MluNI TGGCCA 1 cut(s) 179
MlyI GAGTC 2 cut(s) 124, 519
MmeI TCCRAC 1 cut(s) 403
MnlI CCTC 7 cut(s) 106, 139, 258, 471, 610, 686, 831
Mox20I TGGCCA 1 cut(s) 179
MscI TGGCCA 1 cut(s) 179
MseI TTAA 5 cut(s) 270, 467, 506, 687, 950
MslI CAYNNNNRTG 2 cut(s) 146, 361
Msp20I TGGCCA 1 cut(s) 179
MunI CAATTG 1 cut(s) 580
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 7 cut(s) 14, 34, 70, 79, 133, 636, 996
NdeI CATATG 1 cut(s) 969
NdeII GATC 4 cut(s) 217, 442, 865, 1057
NlaIII CATG 4 cut(s) 517, 605, 638, 1004
NlaIV GGNNCC 1 cut(s) 170
NmeAIII GCCGAG 2 cut(s) 112, 433
OliI CACNNNNGTG 1 cut(s) 146
PciSI GCTCTTC 3 cut(s) 245, 851, 881
PfeI GAWTC 1 cut(s) 568
PflMI CCANNNNNTGG 1 cut(s) 1033
PkrI GCNGC 9 cut(s) 7, 81, 126, 140, 214, 355, 451, 459, 819
PleI GAGTC 2 cut(s) 124, 518
PpsI GAGTC 2 cut(s) 124, 518
PsiI TTATAA 1 cut(s) 552
Psp124BI GAGCTC 2 cut(s) 240, 252
PspN4I GGNNCC 1 cut(s) 170
PspPI GGNCC 2 cut(s) 223, 431
PstI CTGCAG 1 cut(s) 613
PsuI RGATCY 1 cut(s) 442
RsaI GTAC 5 cut(s) 44, 385, 532, 728, 832
RsaNI GTAC 5 cut(s) 43, 384, 531, 727, 831
RseI CAYNNNNRTG 2 cut(s) 146, 361
SacI GAGCTC 2 cut(s) 240, 252
SapI GCTCTTC 3 cut(s) 245, 851, 881
SaqAI TTAA 5 cut(s) 270, 467, 506, 687, 950
SatI GCNGC 9 cut(s) 6, 80, 125, 139, 213, 354, 450, 458, 818
Sau3AI GATC 4 cut(s) 217, 442, 865, 1057
Sau96I GGNCC 2 cut(s) 223, 431
ScaI AGTACT 1 cut(s) 832
SchI GAGTC 2 cut(s) 124, 519
SduI GDGCHC 6 cut(s) 89, 240, 252, 863, 893, 1045
SfaNI GCATC 2 cut(s) 264, 745
SfcI CTRYAG 1 cut(s) 609
SmiMI CAYNNNNRTG 2 cut(s) 146, 361
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 3 cut(s) 580, 653, 947
SsiI CCGC 6 cut(s) 5, 26, 35, 95, 138, 213
SspI AATATT 1 cut(s) 559
SspMI CTAG 3 cut(s) 792, 852, 941
SstI GAGCTC 2 cut(s) 240, 252
TaaI ACNGT 7 cut(s) 206, 488, 648, 704, 717, 760, 788
TaiI ACGT 1 cut(s) 111
TaqI TCGA 4 cut(s) 246, 762, 868, 1060
TasI AATT 3 cut(s) 580, 653, 947
TatI WGTACW 2 cut(s) 726, 830
TauI GCSGC 3 cut(s) 8, 141, 215
TfiI GAWTC 1 cut(s) 568
Tru1I TTAA 5 cut(s) 270, 467, 506, 687, 950
Tru9I TTAA 5 cut(s) 270, 467, 506, 687, 950
TscAI CASTG 5 cut(s) 148, 579, 707, 868, 898
TseI GCWGC 6 cut(s) 79, 124, 353, 449, 457, 817
TspDTI ATGAA 5 cut(s) 232, 590, 687, 739, 913
TspGWI ACGGA 1 cut(s) 1060
TspRI CASTG 5 cut(s) 148, 579, 707, 868, 898
Van91I CCANNNNNTGG 1 cut(s) 1033
XspI CTAG 3 cut(s) 792, 852, 941
ZrmI AGTACT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.