RchiOBHm_Chr2g0173611

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006460
Physical Location & Seq
Forward (+)
86545934 .. 86549509
3576 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54081

Sequence Viewer

Length: 1107 bp
ATGGGTGGCGTGACGTCATCCATGGCGGCCAAGTTCGCCTTTTTCCCGCCGAACCCGCCGTCGTACAAGCTCATCACCGACGAGCTGACGGGGCTGTTGCTGCTGAGCCCATTTCCGCACAGGGAAAACGTGGAGGTTCTGAAGCTGCCGACTCGGCGCGGCACTGAGGTGGTGGCTATTTACATTAGGCACCCTTTGGCCACTTCTACTCTGCTTTACTCTCACGGCAACGCCGCCGATCTGGGCCAGATGTATGAGCTCTTCATCGAGCTCAGCATCCATTTGAGGGTTAATCTCATGGGGTATGACTATTCAGGGTATGGGCAATCATCTGGAAAGGCAAGTGAGCAGAATACATACGCAGATATTGAAGCTGCATATAAGTGTCTTGAAGAAAGCTATGGTACCAAGCAGGAAGATATCATTCTCTATGGTCAATCTGTTGGAAGTGGCCCAACTTTGGATCTAGCTGCTCGGCTGCCTCAGTTAAGAGCTGTTGTTCTGCACAGTCCCATCCTATCTGGTTTAAGAGTCATGTATCCTGTAAAGCGTACATACTGGTTTGACATTTATAAGAATATTGATAAGATTCCACTGGTCAATTGTCCTGTTCTCATCATTCATGGAACTGCAGATGAAGTAGTTGACTGCTCTCATGGCAAGCAACTGTGGGAGTTATGTAAAGAGAAGTATGAACCACTATGGCTTAAAGGAGGCAACCACTGTGATATAGAACTGTATCCCGAGTACATCAGGCATCTCAAGAAGTTCATTTCAACTGTCGAGAAGTCTCCTTCCCAAAGATACAGTTCTAGGAGAAGCACAGATCAATTTGAGCAGCCTCGGAAGAGTACTGATGTTTACGAAGTTTCTAGAAAGAGCACTGATCGAAGAGATAAACCAAAGAAGAGCACTGATAGACCTGAGAAACTGAAAAACCAGTTCATTAGTGCTGATAAACTAGAAAAATTAAAAGTATCCTTTGACCATATGGACAGGTCTCGGAGAAGCGTGGACTGCCATGAGAAGTCCAGAAAAAGTGTCGACCACCAACTGGATAGAGCACGGAAGAGTGTGGATCGATTGGATAGACTACGGAGTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

368

Amino Acids

42.12

Weight (kDa)

9.05

Isoelectric Point (pI)

50.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 71 - 175 3.7e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 71 - 171 1.3e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
AatII GACGTC 1 cut(s) 17
Acc65I GGTACC 1 cut(s) 404
AccB1I GGYRCC 2 cut(s) 189, 404
AccB7I CCANNNNNTGG 1 cut(s) 1054
AccI GTMKAC 1 cut(s) 1044
AccII CGCG 1 cut(s) 159
AciI CCGC 6 cut(s) 26, 47, 56, 116, 159, 234
AclWI GGATC 2 cut(s) 471, 1086
AcoI YGGCCR 2 cut(s) 27, 198
AcuI CTGAAG 1 cut(s) 161
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 5 cut(s) 65, 406, 553, 749, 853
AfiI CCNNNNNNNGG 3 cut(s) 286, 460, 1054
AgsI TTSAA 3 cut(s) 371, 392, 777
AleI CACNNNNGTG 1 cut(s) 167
AluBI AGCT 9 cut(s) 70, 85, 145, 259, 271, 374, 399, 470, 494
AluI AGCT 9 cut(s) 70, 85, 145, 259, 271, 374, 399, 470, 494
Alw21I GWGCWC 5 cut(s) 261, 273, 884, 914, 1066
Alw26I GTCTC 2 cut(s) 795, 1005
AlwI GGATC 2 cut(s) 471, 1086
Ama87I CYCGRG 1 cut(s) 743
AoxI GGCC 4 cut(s) 27, 198, 244, 451
ApeKI GCWGC 6 cut(s) 100, 145, 374, 470, 478, 838
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
Asp718I GGTACC 1 cut(s) 404
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 2 cut(s) 244, 452
AsuHPI GGTGA 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 743
BalI TGGCCA 1 cut(s) 200
BanI GGYRCC 2 cut(s) 189, 404
BanII GRGCYC 3 cut(s) 110, 261, 273
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
Bbv12I GWGCWC 5 cut(s) 261, 273, 884, 914, 1066
BbvI GCAGC 6 cut(s) 87, 132, 361, 457, 465, 850
BccI CCATC 1 cut(s) 521
BceAI ACGGC 2 cut(s) 43, 241
BciVI GTATCC 3 cut(s) 549, 750, 988
BcoDI GTCTC 2 cut(s) 795, 1005
BfaI CTAG 4 cut(s) 467, 813, 873, 962
BfmI CTRYAG 1 cut(s) 630
BfuI GTATCC 3 cut(s) 549, 750, 988
BglI GCCNNNNNGGC 1 cut(s) 154
BisI GCNGC 9 cut(s) 27, 101, 146, 160, 234, 375, 471, 479, 839
BlpI GCTNAGC 2 cut(s) 104, 272
BlsI GCNGC 9 cut(s) 28, 102, 147, 161, 235, 376, 472, 480, 840
BmcAI AGTACT 1 cut(s) 853
BmeT110I CYCGRG 1 cut(s) 743
BmgT120I GGNCC 2 cut(s) 244, 452
BmiI GGNNCC 2 cut(s) 191, 406
BmsI GCATC 2 cut(s) 285, 766
Bpu1102I GCTNAGC 2 cut(s) 104, 272
BpuEI CTTGAG 1 cut(s) 746
Bsa29I ATCGAT 1 cut(s) 1081
BsaHI GRCGYC 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 1005
BsaJI CCNNGG 2 cut(s) 21, 842
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 460, 1054
Bse1I ACTGG 4 cut(s) 563, 600, 940, 1059
BseCI ATCGAT 1 cut(s) 1081
BseDI CCNNGG 2 cut(s) 21, 842
BseGI GGATG 3 cut(s) 17, 276, 513
BseLI CCNNNNNNNGG 3 cut(s) 286, 460, 1054
BseMII CTCAG 5 cut(s) 95, 156, 286, 497, 915
BseNI ACTGG 4 cut(s) 563, 600, 940, 1059
BseXI GCAGC 6 cut(s) 87, 132, 361, 457, 465, 850
BsgI GTGCAG 1 cut(s) 488
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 4 cut(s) 29, 200, 246, 453
BshNI GGYRCC 2 cut(s) 189, 404
BshVI ATCGAT 1 cut(s) 1081
BsiHKAI GWGCWC 5 cut(s) 261, 273, 884, 914, 1066
BsiHKCI CYCGRG 1 cut(s) 743
BslFI GGGAC 1 cut(s) 495
BslI CCNNNNNNNGG 3 cut(s) 286, 460, 1054
BsmAI GTCTC 2 cut(s) 795, 1005
BsmFI GGGAC 1 cut(s) 495
BsnI GGCC 4 cut(s) 29, 200, 246, 453
Bso31I GGTCTC 1 cut(s) 1005
BsoBI CYCGRG 1 cut(s) 743
Bsp1286I GDGCHC 6 cut(s) 110, 261, 273, 884, 914, 1066
Bsp143I GATC 5 cut(s) 238, 463, 826, 886, 1078
Bsp1720I GCTNAGC 2 cut(s) 104, 272
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 6 cut(s) 26, 47, 56, 116, 159, 234
BspANI GGCC 4 cut(s) 29, 200, 246, 453
BspCNI CTCAG 5 cut(s) 96, 157, 285, 496, 916
BspDI ATCGAT 1 cut(s) 1081
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 2 cut(s) 191, 406
BspMAI CTGCAG 1 cut(s) 634
BspPI GGATC 2 cut(s) 471, 1086
BspQI GCTCTTC 2 cut(s) 266, 902
BspT107I GGYRCC 2 cut(s) 189, 404
BspTNI GGTCTC 1 cut(s) 1005
BsrI ACTGG 4 cut(s) 563, 600, 940, 1059
BssECI CCNNGG 2 cut(s) 21, 842
BssMI GATC 5 cut(s) 238, 463, 826, 886, 1078
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 21
Bst4CI ACNGT 6 cut(s) 509, 669, 725, 738, 781, 809
Bst6I CTCTTC 5 cut(s) 266, 842, 886, 902, 1064
BstACI GRCGYC 1 cut(s) 14
BstC8I GCNNGC 1 cut(s) 662
BstDEI CTNAG 5 cut(s) 104, 165, 272, 483, 924
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 3 cut(s) 17, 276, 513
BstFNI CGCG 1 cut(s) 159
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 5 cut(s) 241, 466, 829, 889, 1081
BstMAI GTCTC 2 cut(s) 795, 1005
BstMBI GATC 5 cut(s) 238, 463, 826, 886, 1078
BstMWI GCNNNNNNNGC 7 cut(s) 35, 55, 91, 100, 154, 657, 1017
BstSFI CTRYAG 1 cut(s) 630
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 6 cut(s) 87, 132, 361, 457, 465, 850
BstX2I RGATCY 1 cut(s) 463
BstYI RGATCY 1 cut(s) 463
Bsu15I ATCGAT 1 cut(s) 1081
BsuI GTATCC 3 cut(s) 549, 750, 988
BsuRI GGCC 4 cut(s) 29, 200, 246, 453
BsuTUI ATCGAT 1 cut(s) 1081
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 3 cut(s) 17, 276, 513
BtsIMutI CAGTG 5 cut(s) 162, 593, 721, 882, 912
Cac8I GCNNGC 1 cut(s) 662
CfoI GCGC 1 cut(s) 159
Cfr13I GGNCC 2 cut(s) 244, 452
ClaI ATCGAT 1 cut(s) 1081
Csp6I GTAC 5 cut(s) 64, 405, 552, 748, 852
CviAII CATG 6 cut(s) 22, 298, 535, 623, 656, 1022
CviQI GTAC 5 cut(s) 64, 405, 552, 748, 852
DdeI CTNAG 5 cut(s) 104, 165, 272, 483, 924
DpnI GATC 5 cut(s) 240, 465, 828, 888, 1080
DpnII GATC 5 cut(s) 238, 463, 826, 886, 1078
EaeI YGGCCR 2 cut(s) 27, 198
Eam1104I CTCTTC 5 cut(s) 266, 842, 886, 902, 1064
EarI CTCTTC 5 cut(s) 266, 842, 886, 902, 1064
Ecl136II GAGCTC 2 cut(s) 259, 271
Eco130I CCWWGG 1 cut(s) 21
Eco24I GRGCYC 3 cut(s) 110, 261, 273
Eco31I GGTCTC 1 cut(s) 1005
Eco32I GATATC 1 cut(s) 421
Eco53kI GAGCTC 2 cut(s) 259, 271
Eco57I CTGAAG 1 cut(s) 161
Eco88I CYCGRG 1 cut(s) 743
EcoICRI GAGCTC 2 cut(s) 259, 271
EcoRV GATATC 1 cut(s) 421
EcoT14I CCWWGG 1 cut(s) 21
EcoT38I GRGCYC 3 cut(s) 110, 261, 273
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 6 cut(s) 25, 301, 538, 626, 659, 1025
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FaqI GGGAC 1 cut(s) 495
FatI CATG 6 cut(s) 21, 297, 534, 622, 655, 1021
FauI CCCGC 2 cut(s) 54, 63
FauNDI CATATG 1 cut(s) 990
FblI GTMKAC 1 cut(s) 1044
Fnu4HI GCNGC 9 cut(s) 27, 101, 146, 160, 234, 375, 471, 479, 839
FokI GGATG 3 cut(s) 4, 263, 500
FriOI GRGCYC 3 cut(s) 110, 261, 273
Fsp4HI GCNGC 9 cut(s) 27, 101, 146, 160, 234, 375, 471, 479, 839
FspBI CTAG 4 cut(s) 467, 813, 873, 962
GlaI GCGC 1 cut(s) 158
GluI GCNGC 9 cut(s) 27, 101, 146, 160, 234, 375, 471, 479, 839
HaeIII GGCC 4 cut(s) 29, 200, 246, 453
HhaI GCGC 1 cut(s) 159
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 6 cut(s) 25, 301, 538, 626, 659, 1025
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HincII GTYRAC 2 cut(s) 646, 1045
HindII GTYRAC 2 cut(s) 646, 1045
HinfI GANTC 3 cut(s) 151, 531, 589
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 4 cut(s) 646, 862, 1015, 1045
Hpy188I TCNGA 3 cut(s) 141, 846, 1005
Hpy188III TCNNGA 7 cut(s) 333, 389, 743, 763, 784, 873, 1032
Hpy8I GTNNAC 4 cut(s) 646, 862, 1015, 1045
Hpy99I CGWCG 2 cut(s) 64, 83
HpyAV CCTTC 1 cut(s) 804
HpyCH4III ACNGT 6 cut(s) 509, 669, 725, 738, 781, 809
HpyCH4IV ACGT 2 cut(s) 14, 129
HpyCH4V TGCA 3 cut(s) 377, 505, 632
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 55, 91, 100, 154, 657, 1017
HpyF3I CTNAG 5 cut(s) 104, 165, 272, 483, 924
HpySE526I ACGT 2 cut(s) 14, 129
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 6 cut(s) 25, 301, 538, 626, 659, 1025
HspAI GCGC 1 cut(s) 157
KpnI GGTACC 1 cut(s) 408
Kzo9I GATC 5 cut(s) 238, 463, 826, 886, 1078
LguI GCTCTTC 2 cut(s) 266, 902
Lsp1109I GCAGC 6 cut(s) 87, 132, 361, 457, 465, 850
LweI GCATC 2 cut(s) 285, 766
MaeI CTAG 4 cut(s) 467, 813, 873, 962
MaeII ACGT 2 cut(s) 14, 129
MaeIII GTNAC 1 cut(s) 10
MalI GATC 5 cut(s) 240, 465, 828, 888, 1080
MboI GATC 5 cut(s) 238, 463, 826, 886, 1078
MboII GAAGA 7 cut(s) 253, 404, 428, 859, 903, 919, 1081
MfeI CAATTG 1 cut(s) 601
MflI RGATCY 1 cut(s) 463
MhlI GDGCHC 6 cut(s) 110, 261, 273, 884, 914, 1066
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 3 cut(s) 601, 830, 968
MluNI TGGCCA 1 cut(s) 200
MlyI GAGTC 2 cut(s) 145, 540
MmeI TCCRAC 1 cut(s) 424
MnlI CCTC 6 cut(s) 127, 160, 279, 492, 707, 852
Mox20I TGGCCA 1 cut(s) 200
MscI TGGCCA 1 cut(s) 200
MseI TTAA 5 cut(s) 291, 488, 527, 708, 971
MslI CAYNNNNRTG 2 cut(s) 167, 382
Msp20I TGGCCA 1 cut(s) 200
MunI CAATTG 1 cut(s) 601
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 7 cut(s) 35, 55, 91, 100, 154, 657, 1017
NcoI CCATGG 1 cut(s) 21
NdeI CATATG 1 cut(s) 990
NdeII GATC 5 cut(s) 238, 463, 826, 886, 1078
NlaIII CATG 6 cut(s) 25, 301, 538, 626, 659, 1025
NlaIV GGNNCC 2 cut(s) 191, 406
NmeAIII GCCGAG 2 cut(s) 133, 454
NmuCI GTSAC 1 cut(s) 10
OliI CACNNNNGTG 1 cut(s) 167
PciSI GCTCTTC 2 cut(s) 266, 902
PfeI GAWTC 1 cut(s) 589
PflMI CCANNNNNTGG 1 cut(s) 1054
PkrI GCNGC 9 cut(s) 28, 102, 147, 161, 235, 376, 472, 480, 840
PleI GAGTC 2 cut(s) 145, 539
PpsI GAGTC 2 cut(s) 145, 539
PsiI TTATAA 1 cut(s) 573
Psp124BI GAGCTC 2 cut(s) 261, 273
PspN4I GGNNCC 2 cut(s) 191, 406
PspPI GGNCC 2 cut(s) 244, 452
PstI CTGCAG 1 cut(s) 634
PsuI RGATCY 1 cut(s) 463
RsaI GTAC 5 cut(s) 65, 406, 553, 749, 853
RsaNI GTAC 5 cut(s) 64, 405, 552, 748, 852
RseI CAYNNNNRTG 2 cut(s) 167, 382
SacI GAGCTC 2 cut(s) 261, 273
SalI GTCGAC 1 cut(s) 1043
SapI GCTCTTC 2 cut(s) 266, 902
SaqAI TTAA 5 cut(s) 291, 488, 527, 708, 971
SatI GCNGC 9 cut(s) 27, 101, 146, 160, 234, 375, 471, 479, 839
Sau3AI GATC 5 cut(s) 238, 463, 826, 886, 1078
Sau96I GGNCC 2 cut(s) 244, 452
ScaI AGTACT 1 cut(s) 853
SchI GAGTC 2 cut(s) 145, 540
SduI GDGCHC 6 cut(s) 110, 261, 273, 884, 914, 1066
SfaNI GCATC 2 cut(s) 285, 766
SfcI CTRYAG 1 cut(s) 630
SmiMI CAYNNNNRTG 2 cut(s) 167, 382
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 3 cut(s) 601, 830, 968
SsiI CCGC 6 cut(s) 26, 47, 56, 116, 159, 234
SspI AATATT 1 cut(s) 580
SspMI CTAG 4 cut(s) 467, 813, 873, 962
SstI GAGCTC 2 cut(s) 261, 273
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 6 cut(s) 509, 669, 725, 738, 781, 809
TaiI ACGT 2 cut(s) 17, 132
TaqI TCGA 5 cut(s) 267, 783, 889, 1044, 1081
TasI AATT 3 cut(s) 601, 830, 968
TatI WGTACW 2 cut(s) 747, 851
TauI GCSGC 3 cut(s) 29, 162, 236
TfiI GAWTC 1 cut(s) 589
Tru1I TTAA 5 cut(s) 291, 488, 527, 708, 971
Tru9I TTAA 5 cut(s) 291, 488, 527, 708, 971
TscAI CASTG 5 cut(s) 169, 600, 728, 889, 919
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 6 cut(s) 100, 145, 374, 470, 478, 838
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 6 cut(s) 253, 611, 651, 708, 760, 934
TspGWI ACGGA 1 cut(s) 1081
TspRI CASTG 5 cut(s) 169, 600, 728, 889, 919
Van91I CCANNNNNTGG 1 cut(s) 1054
XbaI TCTAGA 1 cut(s) 872
XmiI GTMKAC 1 cut(s) 1044
XspI CTAG 4 cut(s) 467, 813, 873, 962
ZraI GACGTC 1 cut(s) 15
ZrmI AGTACT 1 cut(s) 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.