FvH4_6g52100

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006460
Physical Location & Seq
Forward (+)
38489737 .. 38493535
3799 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g52100.t3

Sequence Viewer

Length: 1152 bp
ATGGGTGGCGTGACGTCATCCATGGCGGCCAAGTTCGCGTTTTTCCCGCCGAACCCACCGTCGTATAAGCTGATCACCGACGAGCCGACGGGGCTGTTGCTGCTGAGCCCATTTCCCCACAGAGAAAACGTGGAGGTTCTGAAGCTGCCGACTCGTAGAGGCACTGAGGTGGTGGCTATTTACATTAGGCACCCTTTGGCTACTTCCACTCTGCTTTACTCTCACGGCAACGCCGCCGATCTGGGTCAGATGTATGAGCTCTTCATCGAGCTGAGTATCCACTTGAGGGTTAATCTCATGGGGTATGACTATTCAGGGTATGGGCAATCATCTGGAAAGGTCTGCTGCAGAATTGAGCACTTATCTATTCTGTTAACTCCGGCAAGTGAGCAGAATACATACGCAGATATTGAAGCTGCATATAAGTGTCTTGAAGAAAGCTATGGTACCAAGCAGGAAGATATCATTCTCTATGGTCAATCTGTTGGAAGTGGCCCGACTTTGGATCTTGCTGCTCGGTTGCCTCAGTTAAGAGCTGTTGTCCTGCATAGTCCCATACTATCTGGTTTAAGAGTCATGTATCCTGTAAAGCGCACATACTGGTTTGACATTTATAAGAATATTGATAAAATTCCACTGGTCAGTTGTCCTGTTCTCATCATTCATGGAACTGCAGATGAAGTAGTTGATTGCTCTCATGGCAAGCAACTGTGGGAGCTATGTAAAGAGAAGTATGAACCGCTATGGCTTAAAGGAGGCAATCACTGTGATTTAGAACTGTATCCTGAGTACATTAGGCATCTGAAGAAGTTCATATCAACTGTCGAGAAGTCTCCGTCCCAAAGGTTCAGTTCTAGGAGAAGCACAGACCAGTTTGAGCAGCCTCGGAAGAGTACTGATGTTTACGAAGTTTCTAGGAAGAGCACTGATCGAAGAGAGAAACCAAAGAAGAGCACCACTGATAGACCTGAGAAACTGAAAAACCAGTTCATTAGTGCTGATAAACTAGAAAAGTTAAAATTGTCCTTAGACCATATGGACAGGTCTCGGAGAAGCGTGGACTGCCACGAGAAGTCCAGAAAAAGTGTTGACCACCAATTGGATAGAGCGCGGAAGAGTGTGGATCGATTGGATAGAATACGATCTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

384

Amino Acids

43.73

Weight (kDa)

8.98

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 615
AatII GACGTC 1 cut(s) 17
Acc65I GGTACC 1 cut(s) 446
AccB1I GGYRCC 2 cut(s) 189, 446
AccB7I CCANNNNNTGG 1 cut(s) 1099
AccII CGCG 2 cut(s) 38, 1111
AciI CCGC 5 cut(s) 26, 47, 234, 740, 1111
AclWI GGATC 2 cut(s) 513, 1131
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 1 cut(s) 630
AcuI CTGAAG 2 cut(s) 161, 824
AcyI GRCGYC 1 cut(s) 14
AfaI GTAC 3 cut(s) 448, 791, 895
AfiI CCNNNNNNNGG 3 cut(s) 286, 502, 1099
AgsI TTSAA 2 cut(s) 413, 434
AleI CACNNNNGTG 1 cut(s) 167
AluBI AGCT 8 cut(s) 70, 145, 259, 271, 416, 441, 536, 718
AluI AGCT 8 cut(s) 70, 145, 259, 271, 416, 441, 536, 718
Alw21I GWGCWC 4 cut(s) 261, 360, 926, 956
Alw26I GTCTC 2 cut(s) 837, 1050
AlwI GGATC 2 cut(s) 513, 1131
AoxI GGCC 2 cut(s) 27, 493
ApeKI GCWGC 6 cut(s) 100, 145, 345, 416, 512, 880
ApoI RAATTY 1 cut(s) 630
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
Asp700I GAANNNNTTC 1 cut(s) 809
Asp718I GGTACC 1 cut(s) 446
AspLEI GCGC 2 cut(s) 594, 1111
AspS9I GGNCC 1 cut(s) 494
AsuHPI GGTGA 1 cut(s) 67
BanI GGYRCC 2 cut(s) 189, 446
BanII GRGCYC 2 cut(s) 110, 261
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
BauI CACGAG 1 cut(s) 1067
Bbv12I GWGCWC 4 cut(s) 261, 360, 926, 956
BbvI GCAGC 6 cut(s) 87, 132, 332, 403, 499, 892
BceAI ACGGC 1 cut(s) 241
BciVI GTATCC 3 cut(s) 287, 591, 792
BclI TGATCA 1 cut(s) 72
BcoDI GTCTC 2 cut(s) 837, 1050
BfaI CTAG 3 cut(s) 855, 915, 1007
BfmI CTRYAG 2 cut(s) 346, 672
BfuI GTATCC 3 cut(s) 287, 591, 792
BglI GCCNNNNNGGC 1 cut(s) 91
BisI GCNGC 8 cut(s) 27, 101, 146, 234, 346, 417, 513, 881
BlpI GCTNAGC 1 cut(s) 104
BlsI GCNGC 8 cut(s) 28, 102, 147, 235, 347, 418, 514, 882
BmcAI AGTACT 1 cut(s) 895
BmgT120I GGNCC 1 cut(s) 494
BmiI GGNNCC 2 cut(s) 191, 448
BmsI GCATC 1 cut(s) 808
Bpu1102I GCTNAGC 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 304
Bsa29I ATCGAT 1 cut(s) 1126
BsaHI GRCGYC 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 1050
BsaJI CCNNGG 2 cut(s) 21, 884
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 502, 1099
Bse1I ACTGG 4 cut(s) 605, 642, 871, 985
BseCI ATCGAT 1 cut(s) 1126
BseDI CCNNGG 2 cut(s) 21, 884
BseGI GGATG 1 cut(s) 17
BseLI CCNNNNNNNGG 3 cut(s) 286, 502, 1099
BseMII CTCAG 6 cut(s) 95, 156, 263, 539, 777, 960
BseNI ACTGG 4 cut(s) 605, 642, 871, 985
BseXI GCAGC 6 cut(s) 87, 132, 332, 403, 499, 892
Bsh1236I CGCG 2 cut(s) 38, 1111
BshFI GGCC 2 cut(s) 29, 495
BshNI GGYRCC 2 cut(s) 189, 446
BshVI ATCGAT 1 cut(s) 1126
BsiHKAI GWGCWC 4 cut(s) 261, 360, 926, 956
BsiSI CCGG 1 cut(s) 380
BslFI GGGAC 2 cut(s) 537, 823
BslI CCNNNNNNNGG 3 cut(s) 286, 502, 1099
BsmAI GTCTC 2 cut(s) 837, 1050
BsmFI GGGAC 2 cut(s) 537, 823
BsnI GGCC 2 cut(s) 29, 495
Bso31I GGTCTC 1 cut(s) 1050
Bsp1286I GDGCHC 5 cut(s) 110, 261, 360, 926, 956
Bsp143I GATC 6 cut(s) 72, 238, 505, 928, 1123, 1142
Bsp1720I GCTNAGC 1 cut(s) 104
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 5 cut(s) 26, 47, 234, 740, 1111
BspANI GGCC 2 cut(s) 29, 495
BspCNI CTCAG 6 cut(s) 96, 157, 264, 538, 778, 961
BspDI ATCGAT 1 cut(s) 1126
BspFNI CGCG 2 cut(s) 38, 1111
BspLI GGNNCC 2 cut(s) 191, 448
BspMAI CTGCAG 2 cut(s) 350, 676
BspPI GGATC 2 cut(s) 513, 1131
BspQI GCTCTTC 3 cut(s) 266, 914, 944
BspT107I GGYRCC 2 cut(s) 189, 446
BspTNI GGTCTC 1 cut(s) 1050
BsrI ACTGG 4 cut(s) 605, 642, 871, 985
BssECI CCNNGG 2 cut(s) 21, 884
BssMI GATC 6 cut(s) 72, 238, 505, 928, 1123, 1142
BssNI GRCGYC 1 cut(s) 14
BssSI CACGAG 1 cut(s) 1067
BssT1I CCWWGG 1 cut(s) 21
Bst2BI CACGAG 1 cut(s) 1067
Bst4CI ACNGT 5 cut(s) 60, 711, 767, 780, 823
Bst6I CTCTTC 6 cut(s) 266, 884, 914, 928, 944, 1109
BstACI GRCGYC 1 cut(s) 14
BstC8I GCNNGC 1 cut(s) 704
BstDEI CTNAG 7 cut(s) 104, 165, 272, 525, 786, 969, 1027
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 17
BstFNI CGCG 2 cut(s) 38, 1111
BstHHI GCGC 2 cut(s) 594, 1111
BstKTI GATC 6 cut(s) 75, 241, 508, 931, 1126, 1145
BstMAI GTCTC 2 cut(s) 837, 1050
BstMBI GATC 6 cut(s) 72, 238, 505, 928, 1123, 1142
BstMWI GCNNNNNNNGC 5 cut(s) 35, 91, 100, 699, 1062
BstSFI CTRYAG 2 cut(s) 346, 672
BstUI CGCG 2 cut(s) 38, 1111
BstV1I GCAGC 6 cut(s) 87, 132, 332, 403, 499, 892
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
Bsu15I ATCGAT 1 cut(s) 1126
BsuI GTATCC 3 cut(s) 287, 591, 792
BsuRI GGCC 2 cut(s) 29, 495
BsuTUI ATCGAT 1 cut(s) 1126
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 1 cut(s) 17
BtsIMutI CAGTG 5 cut(s) 162, 635, 763, 924, 957
Cac8I GCNNGC 1 cut(s) 704
CfoI GCGC 2 cut(s) 594, 1111
Cfr13I GGNCC 1 cut(s) 494
ClaI ATCGAT 1 cut(s) 1126
Csp6I GTAC 3 cut(s) 447, 790, 894
CviAII CATG 5 cut(s) 22, 298, 577, 665, 698
CviQI GTAC 3 cut(s) 447, 790, 894
DdeI CTNAG 7 cut(s) 104, 165, 272, 525, 786, 969, 1027
DpnI GATC 6 cut(s) 74, 240, 507, 930, 1125, 1144
DpnII GATC 6 cut(s) 72, 238, 505, 928, 1123, 1142
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 6 cut(s) 266, 884, 914, 928, 944, 1109
EarI CTCTTC 6 cut(s) 266, 884, 914, 928, 944, 1109
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 21
Eco24I GRGCYC 2 cut(s) 110, 261
Eco31I GGTCTC 1 cut(s) 1050
Eco32I GATATC 1 cut(s) 463
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 2 cut(s) 161, 824
EcoICRI GAGCTC 1 cut(s) 259
EcoRV GATATC 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 21
EcoT38I GRGCYC 2 cut(s) 110, 261
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 5 cut(s) 25, 301, 580, 668, 701
FaqI GGGAC 2 cut(s) 537, 823
FatI CATG 5 cut(s) 21, 297, 576, 664, 697
FauI CCCGC 1 cut(s) 54
FauNDI CATATG 1 cut(s) 1035
FbaI TGATCA 1 cut(s) 72
Fnu4HI GCNGC 8 cut(s) 27, 101, 146, 234, 346, 417, 513, 881
FokI GGATG 1 cut(s) 4
FriOI GRGCYC 2 cut(s) 110, 261
Fsp4HI GCNGC 8 cut(s) 27, 101, 146, 234, 346, 417, 513, 881
FspBI CTAG 3 cut(s) 855, 915, 1007
GlaI GCGC 2 cut(s) 593, 1110
GluI GCNGC 8 cut(s) 27, 101, 146, 234, 346, 417, 513, 881
HaeIII GGCC 2 cut(s) 29, 495
HapII CCGG 1 cut(s) 380
HhaI GCGC 2 cut(s) 594, 1111
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 5 cut(s) 25, 301, 580, 668, 701
Hin6I GCGC 2 cut(s) 592, 1109
HinP1I GCGC 2 cut(s) 592, 1109
HincII GTYRAC 2 cut(s) 375, 1090
HindII GTYRAC 2 cut(s) 375, 1090
HinfI GANTC 2 cut(s) 151, 573
HpaI GTTAAC 1 cut(s) 375
HpaII CCGG 1 cut(s) 380
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 4 cut(s) 375, 904, 1060, 1090
Hpy188I TCNGA 5 cut(s) 141, 249, 804, 888, 1050
Hpy188III TCNNGA 5 cut(s) 333, 431, 785, 826, 1077
Hpy8I GTNNAC 4 cut(s) 375, 904, 1060, 1090
Hpy99I CGWCG 3 cut(s) 64, 83, 91
HpyCH4III ACNGT 5 cut(s) 60, 711, 767, 780, 823
HpyCH4IV ACGT 2 cut(s) 14, 129
HpyCH4V TGCA 4 cut(s) 348, 419, 547, 674
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 91, 100, 699, 1062
HpyF3I CTNAG 7 cut(s) 104, 165, 272, 525, 786, 969, 1027
HpySE526I ACGT 2 cut(s) 14, 129
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 5 cut(s) 25, 301, 580, 668, 701
HspAI GCGC 2 cut(s) 592, 1109
KpnI GGTACC 1 cut(s) 450
Ksp22I TGATCA 1 cut(s) 72
KspAI GTTAAC 1 cut(s) 375
Kzo9I GATC 6 cut(s) 72, 238, 505, 928, 1123, 1142
LguI GCTCTTC 3 cut(s) 266, 914, 944
LmnI GCTCC 1 cut(s) 715
Lsp1109I GCAGC 6 cut(s) 87, 132, 332, 403, 499, 892
LweI GCATC 1 cut(s) 808
MaeI CTAG 3 cut(s) 855, 915, 1007
MaeII ACGT 2 cut(s) 14, 129
MaeIII GTNAC 1 cut(s) 10
MalI GATC 6 cut(s) 74, 240, 507, 930, 1125, 1144
MboI GATC 6 cut(s) 72, 238, 505, 928, 1123, 1142
MboII GAAGA 9 cut(s) 253, 446, 470, 817, 901, 931, 945, 961, 1126
MfeI CAATTG 1 cut(s) 1097
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 5 cut(s) 110, 261, 360, 926, 956
MluCI AATT 4 cut(s) 351, 630, 1019, 1097
MlyI GAGTC 2 cut(s) 145, 582
MmeI TCCRAC 1 cut(s) 466
MnlI CCTC 7 cut(s) 127, 152, 160, 279, 534, 749, 894
MroXI GAANNNNTTC 1 cut(s) 809
MseI TTAA 6 cut(s) 291, 374, 530, 569, 750, 1016
MslI CAYNNNNRTG 2 cut(s) 167, 424
MspI CCGG 1 cut(s) 380
MunI CAATTG 1 cut(s) 1097
MvnI CGCG 2 cut(s) 38, 1111
MwoI GCNNNNNNNGC 5 cut(s) 35, 91, 100, 699, 1062
NcoI CCATGG 1 cut(s) 21
NdeI CATATG 1 cut(s) 1035
NdeII GATC 6 cut(s) 72, 238, 505, 928, 1123, 1142
NlaIII CATG 5 cut(s) 25, 301, 580, 668, 701
NlaIV GGNNCC 2 cut(s) 191, 448
NmuCI GTSAC 1 cut(s) 10
OliI CACNNNNGTG 1 cut(s) 167
PciSI GCTCTTC 3 cut(s) 266, 914, 944
PdmI GAANNNNTTC 1 cut(s) 809
PflMI CCANNNNNTGG 1 cut(s) 1099
PkrI GCNGC 8 cut(s) 28, 102, 147, 235, 347, 418, 514, 882
PleI GAGTC 2 cut(s) 145, 581
PpsI GAGTC 2 cut(s) 145, 581
PsiI TTATAA 1 cut(s) 615
Psp124BI GAGCTC 1 cut(s) 261
PspN4I GGNNCC 2 cut(s) 191, 448
PspPI GGNCC 1 cut(s) 494
PstI CTGCAG 2 cut(s) 350, 676
PsuI RGATCY 1 cut(s) 505
RsaI GTAC 3 cut(s) 448, 791, 895
RsaNI GTAC 3 cut(s) 447, 790, 894
RseI CAYNNNNRTG 2 cut(s) 167, 424
SacI GAGCTC 1 cut(s) 261
SapI GCTCTTC 3 cut(s) 266, 914, 944
SaqAI TTAA 6 cut(s) 291, 374, 530, 569, 750, 1016
SatI GCNGC 8 cut(s) 27, 101, 146, 234, 346, 417, 513, 881
Sau3AI GATC 6 cut(s) 72, 238, 505, 928, 1123, 1142
Sau96I GGNCC 1 cut(s) 494
ScaI AGTACT 1 cut(s) 895
SchI GAGTC 2 cut(s) 145, 582
SduI GDGCHC 5 cut(s) 110, 261, 360, 926, 956
SfaNI GCATC 1 cut(s) 808
SfcI CTRYAG 2 cut(s) 346, 672
SmiMI CAYNNNNRTG 2 cut(s) 167, 424
SmlI CTYRAG 1 cut(s) 283
SmoI CTYRAG 1 cut(s) 283
Sse9I AATT 4 cut(s) 351, 630, 1019, 1097
SsiI CCGC 5 cut(s) 26, 47, 234, 740, 1111
SspI AATATT 1 cut(s) 622
SspMI CTAG 3 cut(s) 855, 915, 1007
SstI GAGCTC 1 cut(s) 261
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 5 cut(s) 60, 711, 767, 780, 823
TaiI ACGT 2 cut(s) 17, 132
TaqI TCGA 4 cut(s) 267, 825, 931, 1126
TasI AATT 4 cut(s) 351, 630, 1019, 1097
TatI WGTACW 2 cut(s) 789, 893
TauI GCSGC 2 cut(s) 29, 236
Tru1I TTAA 6 cut(s) 291, 374, 530, 569, 750, 1016
Tru9I TTAA 6 cut(s) 291, 374, 530, 569, 750, 1016
TscAI CASTG 5 cut(s) 169, 642, 770, 931, 964
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 6 cut(s) 100, 145, 345, 416, 512, 880
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 6 cut(s) 253, 653, 693, 750, 802, 979
TspGWI ACGGA 1 cut(s) 825
TspRI CASTG 5 cut(s) 169, 642, 770, 931, 964
Van91I CCANNNNNTGG 1 cut(s) 1099
XapI RAATTY 1 cut(s) 630
XmnI GAANNNNTTC 1 cut(s) 809
XspI CTAG 3 cut(s) 855, 915, 1007
ZraI GACGTC 1 cut(s) 15
ZrmI AGTACT 1 cut(s) 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.