AT5G16170

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008998
Physical Location & Seq
Forward (+)
5277787 .. 5279821
2035 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16170.1

Sequence Viewer

Length: 1236 bp
ATGAGCATTAGTGATCATAAGAGCAAGAAAGAAGAAGAACAGTACCCAATGATCACAAAGCCCTTGAGATTGATTCTCTTACAAAAGGGAAGTTATGTCTTAAGCTCACTCTTTTTTGTATTCGGTTTCTCGGTTGGTCTCTTCCTTTGTCTGCAACTAAAAGCCGTACACATGTCTACGACGACGACACAACGACAACCCTTATGGTCTACCTTATTATTCAACCACACGGTCCACACCACAGAGATGGATATCAAACAAGAGTTGCAACTACAAGTACTTCAACACAACATGAGTGATCAAGAACTCTTCACCAAGGCCTCGTCATTATCATCACCAACATCATCCTTATCGTCATCATCATGGTTAGGGAGGAGACATAATAATGATGGGAAGATGGCTGTGAAGGTGGCTTTCATGTTCATGACAGGTGGAAGACTCCCATTGGCTGGCTTGTGGGAAAAGTTCTTTGAAGGACATGAAGGGTTTTATTCCATATATGTTCATACTAATCCATCTTTTCAAGATTCTTTCCCCGAAACTTCCGTCTTCTATTCAAGAAGAATCCCAAGTCAGCCGGTGTATTGGGGAACATCATCAATGGTAGACGCAGAGAAAAGACTATTAGCCAACGCACTTCTCGACGAATCCAACCAAAGATTCGTCCTCTTGTCCGATTCTTGCATCCCACTCTACAATTTCACAACCATCTACGATTACTTAACCGGCACCAATCTCAGCTTCATTGGCTCATTCGACGACCCAAGAAAATCCGGTAGAGGCCGTTACAACCACACAATGTATCCACACATCAACATCACACATTGGAGAAAAGGATCACAGTGGTTTGAAACAACTCGAGAACTTGCTCTTCATATAATCGAAGACACGGTTTACTACAAAATATTCGATCAACATTGTAAACCGCCTTGTTACATGGACGAGCATTATATCCCAACTCTCGTTCACATGTTGCATGGAGAGATGAGCGCTAACCGGACCTTGACGTGGGTTGATTGGTCCAAAGCTGGTCCACATCCTGGCCGGTTTATTTGGCCTGATATAACCGATGAGTTTCTTAACCGAATCCGGTTTAAGGAGGAGTGTGTCTATTTTGGCCGTGGTGGAGAGAATGTTACAACTTCCAAGTGTTTTTTATTTGCGAGGAAATTTACAGCCGAAACTTTAGAACCATTGTTAAGAATTTCGCCAATAGTTCTTGGTTTTGGTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

411

Amino Acids

47.49

Weight (kDa)

7.72

Isoelectric Point (pI)

49.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 137 - 364 9.5e-85 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 728
AccB7I CCANNNNNTGG 2 cut(s) 449, 1040
AccI GTMKAC 3 cut(s) 176, 209, 606
AciI CCGC 1 cut(s) 926
AclWI GGATC 1 cut(s) 844
AcoI YGGCCR 2 cut(s) 1042, 1117
AcsI RAATTY 2 cut(s) 1169, 1203
AfaI GTAC 3 cut(s) 44, 168, 279
AfeI AGCGCT 1 cut(s) 991
AfiI CCNNNNNNNGG 4 cut(s) 449, 1008, 1040, 1096
AflII CTTAAG 1 cut(s) 100
AflIII ACRYGT 2 cut(s) 171, 969
AgsI TTSAA 6 cut(s) 223, 284, 473, 524, 558, 851
AjiI CACGTC 1 cut(s) 1008
AjnI CCWGG 1 cut(s) 1039
AjuI GAANNNNNNNTTGG 4 cut(s) 644, 676, 725, 757
AluBI AGCT 3 cut(s) 105, 741, 1028
AluI AGCT 3 cut(s) 105, 741, 1028
Alw26I GTCTC 2 cut(s) 143, 370
AlwI GGATC 1 cut(s) 844
Ama87I CYCGRG 1 cut(s) 858
Aor51HI AGCGCT 1 cut(s) 991
AoxI GGCC 5 cut(s) 318, 781, 1042, 1055, 1117
ApoI RAATTY 2 cut(s) 1169, 1203
ArsI GACNNNNNNTTYG 2 cut(s) 531, 563
AspLEI GCGC 1 cut(s) 992
AspS9I GGNCC 5 cut(s) 232, 999, 1020, 1031, 1229
AsuHPI GGTGA 2 cut(s) 304, 327
AvaI CYCGRG 1 cut(s) 858
AvaII GGWCC 5 cut(s) 232, 999, 1020, 1031, 1229
BanI GGYRCC 1 cut(s) 728
BbsI GAAGAC 3 cut(s) 442, 541, 891
BccI CCATC 5 cut(s) 241, 383, 391, 523, 716
BceAI ACGGC 3 cut(s) 149, 768, 1104
BciT130I CCWGG 1 cut(s) 1041
BciVI GTATCC 1 cut(s) 813
BclI TGATCA 3 cut(s) 13, 51, 298
BcoDI GTCTC 2 cut(s) 143, 370
BfoI RGCGCY 1 cut(s) 993
BfrI CTTAAG 1 cut(s) 100
BfuI GTATCC 1 cut(s) 813
BmcAI AGTACT 1 cut(s) 279
Bme1390I CCNGG 1 cut(s) 1041
Bme18I GGWCC 5 cut(s) 232, 999, 1020, 1031, 1229
BmeT110I CYCGRG 1 cut(s) 858
BmgBI CACGTC 1 cut(s) 1008
BmgT120I GGNCC 5 cut(s) 232, 999, 1020, 1031, 1229
BmiI GGNNCC 1 cut(s) 730
BmrFI CCNGG 1 cut(s) 1041
BmsI GCATC 1 cut(s) 693
BpiI GAAGAC 3 cut(s) 442, 541, 891
BpuEI CTTGAG 1 cut(s) 85
BsaBI GATNNNNATC 1 cut(s) 251
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 2 cut(s) 315, 1120
BsaWI WCCGGW 3 cut(s) 773, 996, 1089
BsaXI ACNNNNNCTCC 2 cut(s) 1119, 1149
Bsc4I CCNNNNNNNGG 4 cut(s) 449, 1008, 1040, 1096
Bse118I RCCGGY 3 cut(s) 577, 725, 1044
Bse8I GATNNNNATC 1 cut(s) 251
BseBI CCWGG 1 cut(s) 1041
BseDI CCNNGG 2 cut(s) 315, 1120
BseGI GGATG 3 cut(s) 344, 684, 1036
BseJI GATNNNNATC 1 cut(s) 251
BseLI CCNNNNNNNGG 4 cut(s) 449, 1008, 1040, 1096
BseMII CTCAG 1 cut(s) 751
BseRI GAGGAG 2 cut(s) 388, 1115
BshFI GGCC 5 cut(s) 320, 783, 1044, 1057, 1119
BshNI GGYRCC 1 cut(s) 728
BsiHKCI CYCGRG 1 cut(s) 858
BsiSI CCGG 6 cut(s) 578, 726, 774, 997, 1045, 1090
BslI CCNNNNNNNGG 4 cut(s) 449, 1008, 1040, 1096
BsmAI GTCTC 2 cut(s) 143, 370
BsnI GGCC 5 cut(s) 320, 783, 1044, 1057, 1119
Bso31I GGTCTC 1 cut(s) 143
BsoBI CYCGRG 1 cut(s) 858
Bsp143I GATC 5 cut(s) 13, 51, 298, 836, 910
BspACI CCGC 1 cut(s) 926
BspANI GGCC 5 cut(s) 320, 783, 1044, 1057, 1119
BspCNI CTCAG 1 cut(s) 750
BspHI TCATGA 1 cut(s) 423
BspLI GGNNCC 1 cut(s) 730
BspPI GGATC 1 cut(s) 844
BspQI GCTCTTC 1 cut(s) 876
BspT107I GGYRCC 1 cut(s) 728
BspTI CTTAAG 1 cut(s) 100
BspTNI GGTCTC 1 cut(s) 143
BsrFI RCCGGY 3 cut(s) 577, 725, 1044
BssAI RCCGGY 3 cut(s) 577, 725, 1044
BssECI CCNNGG 2 cut(s) 315, 1120
BssMI GATC 5 cut(s) 13, 51, 298, 836, 910
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 1041
Bst4CI ACNGT 4 cut(s) 42, 232, 843, 892
Bst6I CTCTTC 3 cut(s) 146, 314, 876
BstAFI CTTAAG 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 451
BstDEI CTNAG 1 cut(s) 737
BstDSI CCRYGG 1 cut(s) 1120
BstF5I GGATG 3 cut(s) 344, 684, 1036
BstH2I RGCGCY 1 cut(s) 993
BstHHI GCGC 1 cut(s) 992
BstKTI GATC 5 cut(s) 16, 54, 301, 839, 913
BstMAI GTCTC 2 cut(s) 143, 370
BstMBI GATC 5 cut(s) 13, 51, 298, 836, 910
BstMWI GCNNNNNNNGC 1 cut(s) 747
BstNI CCWGG 1 cut(s) 1041
BstNSI RCATGY 2 cut(s) 175, 973
BstSCI CCNGG 1 cut(s) 1039
BstV2I GAAGAC 3 cut(s) 442, 541, 891
BstXI CCANNNNNNTGG 1 cut(s) 247
BsuI GTATCC 1 cut(s) 813
BsuRI GGCC 5 cut(s) 320, 783, 1044, 1057, 1119
BtgI CCRYGG 1 cut(s) 1120
BtrI CACGTC 1 cut(s) 1008
BtsCI GGATG 3 cut(s) 344, 684, 1036
BtsIMutI CAGTG 1 cut(s) 848
Cac8I GCNNGC 1 cut(s) 451
CciI TCATGA 1 cut(s) 423
CfoI GCGC 1 cut(s) 992
Cfr10I RCCGGY 3 cut(s) 577, 725, 1044
Cfr13I GGNCC 5 cut(s) 232, 999, 1020, 1031, 1229
CseI GACGC 1 cut(s) 617
Csp6I GTAC 3 cut(s) 43, 167, 278
CviAII CATG 9 cut(s) 172, 292, 363, 418, 424, 479, 937, 970, 977
CviQI GTAC 3 cut(s) 43, 167, 278
DdeI CTNAG 1 cut(s) 737
DpnI GATC 5 cut(s) 15, 53, 300, 838, 912
DpnII GATC 5 cut(s) 13, 51, 298, 836, 910
EaeI YGGCCR 2 cut(s) 1042, 1117
Eam1104I CTCTTC 3 cut(s) 146, 314, 876
EarI CTCTTC 3 cut(s) 146, 314, 876
Eco130I CCWWGG 1 cut(s) 315
Eco147I AGGCCT 1 cut(s) 320
Eco31I GGTCTC 1 cut(s) 143
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 5 cut(s) 232, 999, 1020, 1031, 1229
Eco47III AGCGCT 1 cut(s) 991
Eco88I CYCGRG 1 cut(s) 858
EcoRII CCWGG 1 cut(s) 1039
EcoRV GATATC 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 9 cut(s) 175, 295, 366, 421, 427, 482, 940, 973, 980
FatI CATG 9 cut(s) 171, 291, 362, 417, 423, 478, 936, 969, 976
FbaI TGATCA 3 cut(s) 13, 51, 298
FblI GTMKAC 3 cut(s) 176, 209, 606
FokI GGATG 3 cut(s) 331, 671, 1023
GlaI GCGC 1 cut(s) 991
HaeII RGCGCY 1 cut(s) 993
HaeIII GGCC 5 cut(s) 320, 783, 1044, 1057, 1119
HapII CCGG 6 cut(s) 578, 726, 774, 997, 1045, 1090
HgaI GACGC 1 cut(s) 617
HhaI GCGC 1 cut(s) 992
Hin1II CATG 9 cut(s) 175, 295, 366, 421, 427, 482, 940, 973, 980
Hin6I GCGC 1 cut(s) 990
HinP1I GCGC 1 cut(s) 990
HinfI GANTC 8 cut(s) 73, 438, 527, 564, 647, 660, 677, 1086
HpaII CCGG 6 cut(s) 578, 726, 774, 997, 1045, 1090
HphI GGTGA 2 cut(s) 304, 327
Hpy166II GTNNAC 9 cut(s) 169, 177, 210, 235, 607, 895, 923, 967, 1034
Hpy188I TCNGA 1 cut(s) 676
Hpy188III TCNNGA 6 cut(s) 302, 424, 524, 558, 641, 860
Hpy8I GTNNAC 9 cut(s) 169, 177, 210, 235, 607, 895, 923, 967, 1034
Hpy99I CGWCG 4 cut(s) 184, 187, 647, 761
HpyAV CCTTC 3 cut(s) 400, 467, 476
HpyCH4III ACNGT 4 cut(s) 42, 232, 843, 892
HpyCH4IV ACGT 1 cut(s) 1007
HpyCH4V TGCA 4 cut(s) 154, 268, 684, 976
HpyF10VI GCNNNNNNNGC 1 cut(s) 747
HpyF3I CTNAG 1 cut(s) 737
HpySE526I ACGT 1 cut(s) 1007
Hsp92II CATG 9 cut(s) 175, 295, 366, 421, 427, 482, 940, 973, 980
HspAI GCGC 1 cut(s) 990
Ksp22I TGATCA 3 cut(s) 13, 51, 298
Kzo9I GATC 5 cut(s) 13, 51, 298, 836, 910
LguI GCTCTTC 1 cut(s) 876
LweI GCATC 1 cut(s) 693
MaeII ACGT 1 cut(s) 1007
MaeIII GTNAC 3 cut(s) 785, 932, 1135
MalI GATC 5 cut(s) 15, 53, 300, 838, 912
MboI GATC 5 cut(s) 13, 51, 298, 836, 910
MluCI AATT 3 cut(s) 697, 1169, 1203
MlyI GAGTC 1 cut(s) 432
MmeI TCCRAC 1 cut(s) 675
MnlI CCTC 6 cut(s) 331, 366, 677, 773, 1093, 1158
MseI TTAA 5 cut(s) 101, 722, 1080, 1095, 1199
MslI CAYNNNNRTG 4 cut(s) 245, 361, 384, 422
MspCI CTTAAG 1 cut(s) 100
MspI CCGG 6 cut(s) 578, 726, 774, 997, 1045, 1090
MspR9I CCNGG 1 cut(s) 1041
MvaI CCWGG 1 cut(s) 1041
MwoI GCNNNNNNNGC 1 cut(s) 747
NdeII GATC 5 cut(s) 13, 51, 298, 836, 910
NlaIII CATG 9 cut(s) 175, 295, 366, 421, 427, 482, 940, 973, 980
NlaIV GGNNCC 1 cut(s) 730
NspI RCATGY 2 cut(s) 175, 973
PaeR7I CTCGAG 1 cut(s) 858
PagI TCATGA 1 cut(s) 423
PceI AGGCCT 1 cut(s) 320
PciI ACATGT 2 cut(s) 171, 969
PciSI GCTCTTC 1 cut(s) 876
PcsI WCGNNNNNNNCGW 1 cut(s) 639
PfeI GAWTC 7 cut(s) 73, 527, 564, 647, 660, 677, 1086
PflMI CCANNNNNTGG 2 cut(s) 449, 1040
PleI GAGTC 1 cut(s) 432
PpsI GAGTC 1 cut(s) 432
PscI ACATGT 2 cut(s) 171, 969
Psp6I CCWGG 1 cut(s) 1039
PspGI CCWGG 1 cut(s) 1039
PspN4I GGNNCC 1 cut(s) 730
PspPI GGNCC 5 cut(s) 232, 999, 1020, 1031, 1229
RsaI GTAC 3 cut(s) 44, 168, 279
RsaNI GTAC 3 cut(s) 43, 167, 278
RseI CAYNNNNRTG 4 cut(s) 245, 361, 384, 422
SapI GCTCTTC 1 cut(s) 876
SaqAI TTAA 5 cut(s) 101, 722, 1080, 1095, 1199
Sau3AI GATC 5 cut(s) 13, 51, 298, 836, 910
Sau96I GGNCC 5 cut(s) 232, 999, 1020, 1031, 1229
ScaI AGTACT 1 cut(s) 279
SchI GAGTC 1 cut(s) 432
ScrFI CCNGG 1 cut(s) 1041
SetI ASST 8 cut(s) 107, 215, 411, 433, 743, 1004, 1010, 1030
SfaNI GCATC 1 cut(s) 693
Sfr274I CTCGAG 1 cut(s) 858
SinI GGWCC 5 cut(s) 232, 999, 1020, 1031, 1229
SlaI CTCGAG 1 cut(s) 858
SmiMI CAYNNNNRTG 4 cut(s) 245, 361, 384, 422
SmlI CTYRAG 3 cut(s) 64, 100, 858
SmoI CTYRAG 3 cut(s) 64, 100, 858
Sse9I AATT 3 cut(s) 697, 1169, 1203
SseBI AGGCCT 1 cut(s) 320
SsiI CCGC 1 cut(s) 926
SspI AATATT 1 cut(s) 906
StuI AGGCCT 1 cut(s) 320
StyD4I CCNGG 1 cut(s) 1039
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 4 cut(s) 42, 232, 843, 892
TaiI ACGT 1 cut(s) 1010
TaqI TCGA 5 cut(s) 642, 756, 859, 882, 909
TasI AATT 3 cut(s) 697, 1169, 1203
TatI WGTACW 1 cut(s) 277
TfiI GAWTC 7 cut(s) 73, 527, 564, 647, 660, 677, 1086
Tru1I TTAA 5 cut(s) 101, 722, 1080, 1095, 1199
Tru9I TTAA 5 cut(s) 101, 722, 1080, 1095, 1199
TscAI CASTG 1 cut(s) 848
TspDTI ATGAA 6 cut(s) 406, 412, 494, 495, 733, 863
TspGWI ACGGA 1 cut(s) 535
TspRI CASTG 1 cut(s) 848
Van91I CCANNNNNTGG 2 cut(s) 449, 1040
Vha464I CTTAAG 1 cut(s) 100
VpaK11BI GGWCC 5 cut(s) 232, 999, 1020, 1031, 1229
XapI RAATTY 2 cut(s) 1169, 1203
XceI RCATGY 2 cut(s) 175, 973
XhoI CTCGAG 1 cut(s) 858
XmiI GTMKAC 3 cut(s) 176, 209, 606
ZrmI AGTACT 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.