FvH4_7g07190

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008998
Physical Location & Seq
Reverse (-)
7152731 .. 7154066
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g07190.t1

Sequence Viewer

Length: 1233 bp
ATGGCAGCAATATTGGGCAGCAGCAGCAGCAGTAGTAATACTAGCAGTGAGCAACATCATCGAGCTTTTCAATCCAGTCACGTCCTTCATTTCCTCTTCTTTGTTATTGGTTTATCACTCGGCATCCTAGCTACCTTATATTTCCGGAGTTACTCATTGCTCACTTTTCAAGACTCCCTCAACTACATTCCATTCAATCCATCACCAACCCCACCTCCACCTGCGCTGTCGTCACCGCCACTTCTAGTAAATGAAACAACATCATCCTCAGTATCAGTTAATTACAGTACCAATCCTAGTACTGCTAGTGTTTCTTTGAAAGAACATAAGTTGTTAGTACACAACATGAGCGACGAGGAGTTACTTCGGAGAGCATCCATGACCCCTCGAGTTGGATTGCCTTACAAAAGTGTTCCCACTGTGGCTTTTATGTTCTTGACACAAGGGCCTCTACCTTTGGCTCCCTTATGGGACAAATTTTTCGAGGGACACGAGGGTCTATACTCCATCTATGTGCACGCACACCCTTCTTTCAATGAATCGCAGCCTCCAACTTCTGTGTTCTATGGTCGAAGGATTCATAGCCAGGCAGTTTATTGGGGAACTTCCTCGATGTTGGATGCAGAGCGACGACTATTAGCGAATGCACTGCTTGACTTGTCTAATCAAAGATTTGTATTGCTTTCCGAATCTTGCATCCCCTTGTTTAACTTCACAACAACTTATGACTACCTTCTAAATTCAAACCAAAGCTTTCTAAGTGTTTTTGATGATCCGAGGAAGCCTGGTCGAGGGCGTTACAACCCTCAAATGTGGCCACAAATAAACATCACAGATTGGAGGAAAGGATCTCAATGGTTTGAAGTGCACCGAGACCTGGCCATCCACATTGTCTCCGATCGGAAATACTACCCCATCTTTCAGCAACACTGCCACCCTCCTTGTTATATTGATGAGCACTATATTCCAACACTGGTCAACATGTTGTATGCTGATCTCAACTCTGAGAGGAGCATTACATGGGTGGATTGGTCAAGGGGTGGTCCTCATCCAGGGAAGTTTGGATGGCCTGATATTACAGATGAGTTTCTGAATGGAATTCGGTTCGGATCCGAATGCAAATACAACGGCAATATGACTTCAATATGCTTCTTATTTGGTAGAAAGTTTTTGCCTCATGCCCTGGAGCCTTTGTTACGAGTTGCTCCACTGCTGCTTGGATTTGATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

411

Amino Acids

46.59

Weight (kDa)

6.78

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 141 - 367 2.2e-81 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 229
AasI GACNNNNNNGTC 1 cut(s) 495
Acc36I ACCTGC 1 cut(s) 229
AccIII TCCGGA 1 cut(s) 144
AciI CCGC 1 cut(s) 236
AclWI GGATC 5 cut(s) 767, 856, 1104, 1117, 1220
AcoI YGGCCR 2 cut(s) 815, 879
AcsI RAATTY 3 cut(s) 476, 739, 1098
AfaI GTAC 3 cut(s) 289, 301, 339
AfiI CCNNNNNNNGG 4 cut(s) 392, 791, 877, 1052
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 8 cut(s) 71, 170, 196, 319, 535, 744, 863, 1143
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 5 cut(s) 585, 784, 876, 1051, 1182
AluBI AGCT 3 cut(s) 65, 131, 753
AluI AGCT 3 cut(s) 65, 131, 753
Alw21I GWGCWC 3 cut(s) 519, 870, 960
Alw26I GTCTC 2 cut(s) 867, 898
Alw44I GTGCAC 2 cut(s) 515, 866
AlwI GGATC 5 cut(s) 767, 856, 1104, 1117, 1220
Ama87I CYCGRG 1 cut(s) 387
Aor13HI TCCGGA 1 cut(s) 144
AoxI GGCC 4 cut(s) 446, 815, 879, 1067
ApaLI GTGCAC 2 cut(s) 515, 866
ApeKI GCWGC 7 cut(s) 5, 18, 21, 24, 27, 544, 1213
ApoI RAATTY 3 cut(s) 476, 739, 1098
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
AspLEI GCGC 1 cut(s) 226
AspS9I GGNCC 2 cut(s) 446, 1043
AsuHPI GGTGA 2 cut(s) 195, 225
AvaI CYCGRG 1 cut(s) 387
AvaII GGWCC 1 cut(s) 1043
BaeGI GKGCMC 2 cut(s) 519, 870
BalI TGGCCA 2 cut(s) 817, 881
BamHI GGATCC 1 cut(s) 1109
BauI CACGAG 1 cut(s) 491
Bbv12I GWGCWC 3 cut(s) 519, 870, 960
BbvI GCAGC 7 cut(s) 17, 30, 33, 36, 39, 556, 1200
BccI CCATC 5 cut(s) 208, 515, 890, 923, 1059
BceAI ACGGC 1 cut(s) 1144
BcgI CGANNNNNNTGC 4 cut(s) 41, 75, 631, 665
BciT130I CCWGG 5 cut(s) 587, 786, 878, 1053, 1184
BcoDI GTCTC 2 cut(s) 867, 898
BfaI CTAG 6 cut(s) 42, 128, 245, 297, 306, 1231
BfuAI ACCTGC 1 cut(s) 229
BisI GCNGC 7 cut(s) 6, 19, 22, 25, 28, 545, 1214
BlsI GCNGC 7 cut(s) 7, 20, 23, 26, 29, 546, 1215
BmcAI AGTACT 1 cut(s) 301
Bme1390I CCNGG 5 cut(s) 587, 786, 878, 1053, 1184
Bme18I GGWCC 1 cut(s) 1043
BmeT110I CYCGRG 1 cut(s) 387
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 2 cut(s) 446, 1043
BmiI GGNNCC 3 cut(s) 462, 1111, 1188
BmrFI CCNGG 5 cut(s) 587, 786, 878, 1053, 1184
BmsI GCATC 4 cut(s) 132, 383, 610, 705
BpmI CTGGAG 1 cut(s) 1205
BsaI GGTCTC 1 cut(s) 867
BsaJI CCNNGG 3 cut(s) 776, 1052, 1182
BsaWI WCCGGW 1 cut(s) 144
BsaXI ACNNNNNCTCC 6 cut(s) 199, 229, 878, 908, 1178, 1208
Bsc4I CCNNNNNNNGG 4 cut(s) 392, 791, 877, 1052
Bse1I ACTGG 2 cut(s) 75, 978
Bse3DI GCAATG 1 cut(s) 155
BseAI TCCGGA 1 cut(s) 144
BseBI CCWGG 5 cut(s) 587, 786, 878, 1053, 1184
BseDI CCNNGG 3 cut(s) 776, 1052, 1182
BseGI GGATG 8 cut(s) 123, 263, 374, 625, 696, 882, 1048, 1070
BseLI CCNNNNNNNGG 4 cut(s) 392, 791, 877, 1052
BseMI GCAATG 1 cut(s) 155
BseMII CTCAG 2 cut(s) 282, 996
BseNI ACTGG 2 cut(s) 75, 978
BseRI GAGGAG 2 cut(s) 371, 1024
BseSI GKGCMC 2 cut(s) 519, 870
BseXI GCAGC 7 cut(s) 17, 30, 33, 36, 39, 556, 1200
Bsh1285I CGRYCG 1 cut(s) 901
BshFI GGCC 4 cut(s) 448, 817, 881, 1069
BsiEI CGRYCG 1 cut(s) 901
BsiHKAI GWGCWC 3 cut(s) 519, 870, 960
BsiHKCI CYCGRG 1 cut(s) 387
BsiSI CCGG 1 cut(s) 145
BslFI GGGAC 2 cut(s) 485, 501
BslI CCNNNNNNNGG 4 cut(s) 392, 791, 877, 1052
BsmAI GTCTC 2 cut(s) 867, 898
BsmFI GGGAC 2 cut(s) 485, 501
BsmI GAATGC 2 cut(s) 649, 1121
BsnI GGCC 4 cut(s) 448, 817, 881, 1069
Bso31I GGTCTC 1 cut(s) 867
BsoBI CYCGRG 1 cut(s) 387
Bsp1286I GDGCHC 3 cut(s) 519, 870, 960
Bsp13I TCCGGA 1 cut(s) 144
Bsp143I GATC 6 cut(s) 772, 848, 898, 994, 1109, 1225
BspACI CCGC 1 cut(s) 236
BspANI GGCC 4 cut(s) 448, 817, 881, 1069
BspCNI CTCAG 2 cut(s) 281, 997
BspEI TCCGGA 1 cut(s) 144
BspLI GGNNCC 3 cut(s) 462, 1111, 1188
BspMI ACCTGC 1 cut(s) 229
BspPI GGATC 5 cut(s) 767, 856, 1104, 1117, 1220
BspTNI GGTCTC 1 cut(s) 867
BsrDI GCAATG 1 cut(s) 155
BsrI ACTGG 2 cut(s) 75, 978
BssECI CCNNGG 3 cut(s) 776, 1052, 1182
BssMI GATC 6 cut(s) 772, 848, 898, 994, 1109, 1225
BssSI CACGAG 1 cut(s) 491
Bst2BI CACGAG 1 cut(s) 491
Bst2UI CCWGG 5 cut(s) 587, 786, 878, 1053, 1184
Bst4CI ACNGT 2 cut(s) 287, 421
Bst6I CTCTTC 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 3 cut(s) 268, 758, 1005
BstENI CCTNNNNNAGG 2 cut(s) 789, 1050
BstF5I GGATG 8 cut(s) 123, 263, 374, 625, 696, 882, 1048, 1070
BstHHI GCGC 1 cut(s) 226
BstKTI GATC 6 cut(s) 775, 851, 901, 997, 1112, 1228
BstMAI GTCTC 2 cut(s) 867, 898
BstMBI GATC 6 cut(s) 772, 848, 898, 994, 1109, 1225
BstMCI CGRYCG 1 cut(s) 901
BstMWI GCNNNNNNNGC 2 cut(s) 24, 27
BstNI CCWGG 5 cut(s) 587, 786, 878, 1053, 1184
BstNSI RCATGY 1 cut(s) 985
BstSCI CCNGG 5 cut(s) 585, 784, 876, 1051, 1182
BstSLI GKGCMC 2 cut(s) 519, 870
BstV1I GCAGC 7 cut(s) 17, 30, 33, 36, 39, 556, 1200
BstX2I RGATCY 2 cut(s) 848, 1109
BstYI RGATCY 2 cut(s) 848, 1109
BsuRI GGCC 4 cut(s) 448, 817, 881, 1069
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 8 cut(s) 123, 263, 374, 625, 696, 882, 1048, 1070
BtsI GCAGTG 4 cut(s) 52, 647, 928, 1208
BtsIMutI CAGTG 6 cut(s) 52, 417, 647, 928, 971, 1208
BveI ACCTGC 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 519
CfoI GCGC 1 cut(s) 226
Cfr13I GGNCC 2 cut(s) 446, 1043
Csp6I GTAC 3 cut(s) 288, 300, 338
CspCI CAANNNNNGTGG 2 cut(s) 923, 958
CviAII CATG 5 cut(s) 346, 379, 982, 1020, 1178
CviQI GTAC 3 cut(s) 288, 300, 338
DdeI CTNAG 3 cut(s) 268, 758, 1005
DpnI GATC 6 cut(s) 774, 850, 900, 996, 1111, 1227
DpnII GATC 6 cut(s) 772, 848, 898, 994, 1109, 1225
DrdI GACNNNNNNGTC 1 cut(s) 495
DseDI GACNNNNNNGTC 1 cut(s) 495
EaeI YGGCCR 2 cut(s) 815, 879
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
Eco31I GGTCTC 1 cut(s) 867
Eco47I GGWCC 1 cut(s) 1043
Eco88I CYCGRG 1 cut(s) 387
EcoNI CCTNNNNNAGG 2 cut(s) 789, 1050
EcoO109I RGGNCCY 1 cut(s) 446
EcoRI GAATTC 1 cut(s) 1098
EcoRII CCWGG 5 cut(s) 585, 784, 876, 1051, 1182
FaeI CATG 5 cut(s) 349, 382, 985, 1023, 1181
FaqI GGGAC 2 cut(s) 485, 501
FatI CATG 5 cut(s) 345, 378, 981, 1019, 1177
Fnu4HI GCNGC 7 cut(s) 6, 19, 22, 25, 28, 545, 1214
FokI GGATG 8 cut(s) 110, 250, 361, 632, 683, 869, 1035, 1077
Fsp4HI GCNGC 7 cut(s) 6, 19, 22, 25, 28, 545, 1214
FspBI CTAG 6 cut(s) 42, 128, 245, 297, 306, 1231
GlaI GCGC 1 cut(s) 225
GluI GCNGC 7 cut(s) 6, 19, 22, 25, 28, 545, 1214
GsuI CTGGAG 1 cut(s) 1205
HaeIII GGCC 4 cut(s) 448, 817, 881, 1069
HapII CCGG 1 cut(s) 145
HhaI GCGC 1 cut(s) 226
Hin1II CATG 5 cut(s) 349, 382, 985, 1023, 1181
Hin6I GCGC 1 cut(s) 224
HinP1I GCGC 1 cut(s) 224
HincII GTYRAC 1 cut(s) 979
HindII GTYRAC 1 cut(s) 979
HindIII AAGCTT 1 cut(s) 751
HinfI GANTC 4 cut(s) 173, 539, 577, 689
HpaII CCGG 1 cut(s) 145
HphI GGTGA 2 cut(s) 195, 225
Hpy166II GTNNAC 4 cut(s) 340, 517, 868, 979
Hpy188I TCNGA 9 cut(s) 369, 688, 777, 898, 903, 1006, 1092, 1109, 1114
Hpy188III TCNNGA 3 cut(s) 145, 170, 436
Hpy8I GTNNAC 4 cut(s) 340, 517, 868, 979
Hpy99I CGWCG 2 cut(s) 356, 633
HpyAV CCTTC 4 cut(s) 95, 537, 567, 743
HpyCH4III ACNGT 2 cut(s) 287, 421
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 6 cut(s) 517, 623, 647, 696, 868, 1119
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 27
HpyF3I CTNAG 3 cut(s) 268, 758, 1005
HpySE526I ACGT 1 cut(s) 81
Hsp92II CATG 5 cut(s) 349, 382, 985, 1023, 1181
HspAI GCGC 1 cut(s) 224
Kpn2I TCCGGA 1 cut(s) 144
Kzo9I GATC 6 cut(s) 772, 848, 898, 994, 1109, 1225
LmnI GCTCC 4 cut(s) 466, 1011, 1186, 1210
Lsp1109I GCAGC 7 cut(s) 17, 30, 33, 36, 39, 556, 1200
LweI GCATC 4 cut(s) 132, 383, 610, 705
MaeI CTAG 6 cut(s) 42, 128, 245, 297, 306, 1231
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 6 cut(s) 77, 149, 231, 360, 797, 1194
MalI GATC 6 cut(s) 774, 850, 900, 996, 1111, 1227
MboI GATC 6 cut(s) 772, 848, 898, 994, 1109, 1225
MboII GAAGA 1 cut(s) 88
MflI RGATCY 2 cut(s) 848, 1109
MhlI GDGCHC 3 cut(s) 519, 870, 960
MlsI TGGCCA 2 cut(s) 817, 881
MluCI AATT 4 cut(s) 280, 476, 739, 1098
MluNI TGGCCA 2 cut(s) 817, 881
MlyI GAGTC 1 cut(s) 167
MmeI TCCRAC 4 cut(s) 373, 575, 597, 992
Mox20I TGGCCA 2 cut(s) 817, 881
MroI TCCGGA 1 cut(s) 144
MscI TGGCCA 2 cut(s) 817, 881
MseI TTAA 2 cut(s) 279, 708
MslI CAYNNNNRTG 1 cut(s) 512
Msp20I TGGCCA 2 cut(s) 817, 881
MspI CCGG 1 cut(s) 145
MspR9I CCNGG 5 cut(s) 587, 786, 878, 1053, 1184
Mva1269I GAATGC 2 cut(s) 649, 1121
MvaI CCWGG 5 cut(s) 587, 786, 878, 1053, 1184
MwoI GCNNNNNNNGC 2 cut(s) 24, 27
NdeII GATC 6 cut(s) 772, 848, 898, 994, 1109, 1225
NlaIII CATG 5 cut(s) 349, 382, 985, 1023, 1181
NlaIV GGNNCC 3 cut(s) 462, 1111, 1188
NmeAIII GCCGAG 1 cut(s) 99
NmuCI GTSAC 2 cut(s) 77, 231
NspI RCATGY 1 cut(s) 985
PaeR7I CTCGAG 1 cut(s) 387
PaqCI CACCTGC 1 cut(s) 229
PciI ACATGT 1 cut(s) 981
PcsI WCGNNNNNNNCGW 1 cut(s) 489
PctI GAATGC 2 cut(s) 649, 1121
PfeI GAWTC 3 cut(s) 539, 577, 689
PkrI GCNGC 7 cut(s) 7, 20, 23, 26, 29, 546, 1215
Ple19I CGATCG 1 cut(s) 901
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
PscI ACATGT 1 cut(s) 981
Psp6I CCWGG 5 cut(s) 585, 784, 876, 1051, 1182
PspGI CCWGG 5 cut(s) 585, 784, 876, 1051, 1182
PspN4I GGNNCC 3 cut(s) 462, 1111, 1188
PspPI GGNCC 2 cut(s) 446, 1043
PspXI VCTCGAGB 1 cut(s) 387
PsuI RGATCY 2 cut(s) 848, 1109
PvuI CGATCG 1 cut(s) 901
RsaI GTAC 3 cut(s) 289, 301, 339
RsaNI GTAC 3 cut(s) 288, 300, 338
RseI CAYNNNNRTG 1 cut(s) 512
SaqAI TTAA 2 cut(s) 279, 708
SatI GCNGC 7 cut(s) 6, 19, 22, 25, 28, 545, 1214
Sau3AI GATC 6 cut(s) 772, 848, 898, 994, 1109, 1225
Sau96I GGNCC 2 cut(s) 446, 1043
ScaI AGTACT 1 cut(s) 301
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 5 cut(s) 587, 786, 878, 1053, 1184
SduI GDGCHC 3 cut(s) 519, 870, 960
SfaNI GCATC 4 cut(s) 132, 383, 610, 705
Sfr274I CTCGAG 1 cut(s) 387
SinI GGWCC 1 cut(s) 1043
SlaI CTCGAG 1 cut(s) 387
SmiMI CAYNNNNRTG 1 cut(s) 512
SmlI CTYRAG 1 cut(s) 387
SmoI CTYRAG 1 cut(s) 387
Sse9I AATT 4 cut(s) 280, 476, 739, 1098
SsiI CCGC 1 cut(s) 236
SspI AATATT 1 cut(s) 12
SspMI CTAG 6 cut(s) 42, 128, 245, 297, 306, 1231
StyD4I CCNGG 5 cut(s) 585, 784, 876, 1051, 1182
TaaI ACNGT 2 cut(s) 287, 421
TaiI ACGT 1 cut(s) 84
TaqI TCGA 6 cut(s) 61, 388, 483, 571, 611, 790
TasI AATT 4 cut(s) 280, 476, 739, 1098
TatI WGTACW 2 cut(s) 299, 337
TfiI GAWTC 3 cut(s) 539, 577, 689
Tru1I TTAA 2 cut(s) 279, 708
Tru9I TTAA 2 cut(s) 279, 708
TscAI CASTG 6 cut(s) 52, 424, 654, 935, 978, 1215
TseFI GTSAC 2 cut(s) 77, 231
TseI GCWGC 7 cut(s) 5, 18, 21, 24, 27, 544, 1213
Tsp45I GTSAC 2 cut(s) 77, 231
TspDTI ATGAA 4 cut(s) 77, 267, 552, 569
TspRI CASTG 6 cut(s) 52, 424, 654, 935, 978, 1215
VneI GTGCAC 2 cut(s) 515, 866
VpaK11BI GGWCC 1 cut(s) 1043
XagI CCTNNNNNAGG 2 cut(s) 789, 1050
XapI RAATTY 3 cut(s) 476, 739, 1098
XceI RCATGY 1 cut(s) 985
XhoI CTCGAG 1 cut(s) 387
XspI CTAG 6 cut(s) 42, 128, 245, 297, 306, 1231
ZrmI AGTACT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.