Rroxscaffold_6G00391280

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008998
Physical Location & Seq
Forward (+)
8871387 .. 8872766
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00391280.1

Sequence Viewer

Length: 1281 bp
ATGGCAGCGATATTGTGCAGCAGCAGCAGTGCTAGTGAGCAACATCATCGGGCGTTTCAATTCAATTACGTCCTGCATTTCCTCTTCTTTGTTATTGGTTTGTCACTCGGCATCCTGGCTACCTTATATTTCCAGAGTTACTCATTAGTCACTTTTCAAGCATCTCTCAACTCCATTAATAATTTTATTAACACTCCACTAAAACCATCACCCCCATCACAACCTCCGCCTCGACCGAAATTACCACCTACACCTCCACCTCCACCGCCACCGCCGCTTCTGGTACTGTCTTTCTCAACAATTAATGCAACATCATCATCAGTATTAGTTAATTACAGTAGCAGTCTCAGCACTAGTGTTTCTTTGAAAGAACAAAAGCTGTTAGTACACAACATGAGCGACGAGGAGTTACTTTGGAGAGCATCCATGGCCCCTCAAGTTGATTCGCCTTATAACTGTGTTCCGAAAGTGGCTTTCATGTTCTTGACAAAAGGGCCTCTACCTTTGGCTCCCTTATGGGACAAATTTTTTGAGGGACACGAGGGTCTATACTCTATCTATGTGCACGCGCACCCTTCTTTTAATGAATCGCAGCCTCAAACTTCTGTCTTCCATGGTCGAAGAATTAATAGCCAGGCCGTTTACTGGGGAACTTCCTCAATGTTGGATGCAGAGCGACGGCTATTAGCAAATGCACTGCTTGACTTGTCAAATCAAAGATTTGTATTGCTTTCCGAATCGTGCATTCCCTTGTTTAACTTCACGACAACTTATGACTACCTTGTAAACTCAAATCAAAGCTTTCTAAGCGTATTCGACGACCCGAGGAAGCCTGGTCGAGGGCGCTACAACCCTCAAATGTGGCCCCAAATAAACATCACAGATTGGCGGAAAGGATCTCAATGGTTTGAAGTGCACCGAGATCTGGCCGTCCACATTGTCTCCGATCAGAAATACTACCCCATCTTTCAACAATACTGCCACCCTCCTTGTTATATCGATGAGCATTATATTCCAACACTCGTCAACATGTTGTACGCTGATCTCAACTCTGAGAGGAGCATTACATGGGTGGATTGGTCAAGGGGTGGTCCTCATCCTGGGAAATTTGGATGGCCTGATATTACAGATGAGTTTTTGAATGGAATTCGGTTCGGATCGCAGTGCAAATACAACGGCAATACTACTTCATTATGTTTCTTATTTGGTAGGAAGTTTTTGTCTAATGCTCTGGAGCCTTTGTTACGAGTTTCTCCACTGCTGCTTGGCTTTGATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

426

Amino Acids

48.34

Weight (kDa)

7.2

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 157 - 383 2.4e-82 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 453
AasI GACNNNNNNGTC 1 cut(s) 543
AccII CGCG 1 cut(s) 569
AciI CCGC 5 cut(s) 227, 266, 272, 275, 889
AclWI GGATC 3 cut(s) 904, 1165, 1268
AcoI YGGCCR 1 cut(s) 927
AcsI RAATTY 3 cut(s) 524, 1106, 1146
AfaI GTAC 3 cut(s) 285, 387, 1037
AfiI CCNNNNNNNGG 4 cut(s) 645, 839, 925, 1100
AflIII ACRYGT 1 cut(s) 1029
AgsI TTSAA 7 cut(s) 59, 64, 158, 367, 911, 971, 1141
AhlI ACTAGT 1 cut(s) 353
AjnI CCWGG 4 cut(s) 114, 633, 832, 1099
AluBI AGCT 2 cut(s) 379, 801
AluI AGCT 2 cut(s) 379, 801
Alw21I GWGCWC 2 cut(s) 567, 918
Alw26I GTCTC 2 cut(s) 350, 946
Alw44I GTGCAC 2 cut(s) 563, 914
AlwI GGATC 3 cut(s) 904, 1165, 1268
Ama87I CYCGRG 1 cut(s) 823
AoxI GGCC 6 cut(s) 429, 494, 636, 863, 927, 1115
ApaLI GTGCAC 2 cut(s) 563, 914
ApeKI GCWGC 6 cut(s) 5, 18, 21, 24, 592, 1261
ApoI RAATTY 3 cut(s) 524, 1106, 1146
ArsI GACNNNNNNTTYG 2 cut(s) 512, 544
AseI ATTAAT 3 cut(s) 177, 303, 627
AspLEI GCGC 2 cut(s) 571, 846
AspS9I GGNCC 4 cut(s) 430, 494, 864, 1091
AsuHPI GGTGA 1 cut(s) 201
AvaI CYCGRG 1 cut(s) 823
AvaII GGWCC 1 cut(s) 1091
BaeGI GKGCMC 2 cut(s) 567, 918
BauI CACGAG 1 cut(s) 539
BbsI GAAGAC 1 cut(s) 601
Bbv12I GWGCWC 2 cut(s) 567, 918
BbvI GCAGC 6 cut(s) 17, 30, 33, 36, 604, 1248
BccI CCATC 4 cut(s) 214, 223, 971, 1107
BceAI ACGGC 4 cut(s) 623, 695, 914, 1192
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BciT130I CCWGG 4 cut(s) 116, 635, 834, 1101
BcoDI GTCTC 2 cut(s) 350, 946
BcuI ACTAGT 1 cut(s) 353
BfaI CTAG 3 cut(s) 33, 354, 1279
BfoI RGCGCY 1 cut(s) 847
BglII AGATCT 1 cut(s) 922
BisI GCNGC 7 cut(s) 6, 19, 22, 25, 275, 593, 1262
BlsI GCNGC 7 cut(s) 7, 20, 23, 26, 276, 594, 1263
Bme1390I CCNGG 4 cut(s) 116, 635, 834, 1101
Bme18I GGWCC 1 cut(s) 1091
BmeT110I CYCGRG 1 cut(s) 823
BmgT120I GGNCC 4 cut(s) 430, 494, 864, 1091
BmiI GGNNCC 4 cut(s) 432, 510, 866, 1236
BmrFI CCNGG 4 cut(s) 116, 635, 834, 1101
BmrI ACTGGG 1 cut(s) 655
BmsI GCATC 4 cut(s) 120, 170, 431, 658
BmuI ACTGGG 1 cut(s) 655
BpiI GAAGAC 1 cut(s) 601
BpmI CTGGAG 1 cut(s) 1253
BpuEI CTTGAG 1 cut(s) 420
Bsa29I ATCGAT 1 cut(s) 999
BsaJI CCNNGG 4 cut(s) 426, 613, 824, 1100
BsaXI ACNNNNNCTCC 4 cut(s) 926, 956, 1226, 1256
Bsc4I CCNNNNNNNGG 4 cut(s) 645, 839, 925, 1100
Bse1I ACTGG 1 cut(s) 650
BseBI CCWGG 4 cut(s) 116, 635, 834, 1101
BseCI ATCGAT 1 cut(s) 999
BseDI CCNNGG 4 cut(s) 426, 613, 824, 1100
BseGI GGATG 5 cut(s) 111, 422, 673, 1096, 1118
BseLI CCNNNNNNNGG 4 cut(s) 645, 839, 925, 1100
BseMII CTCAG 2 cut(s) 361, 1044
BseNI ACTGG 1 cut(s) 650
BseRI GAGGAG 2 cut(s) 419, 1072
BseSI GKGCMC 2 cut(s) 567, 918
BseXI GCAGC 6 cut(s) 17, 30, 33, 36, 604, 1248
BsgI GTGCAG 1 cut(s) 37
Bsh1236I CGCG 1 cut(s) 569
Bsh1285I CGRYCG 1 cut(s) 236
BshFI GGCC 6 cut(s) 431, 496, 638, 865, 929, 1117
BshVI ATCGAT 1 cut(s) 999
BsiEI CGRYCG 1 cut(s) 236
BsiHKAI GWGCWC 2 cut(s) 567, 918
BsiHKCI CYCGRG 1 cut(s) 823
BslFI GGGAC 2 cut(s) 533, 549
BslI CCNNNNNNNGG 4 cut(s) 645, 839, 925, 1100
BsmAI GTCTC 2 cut(s) 350, 946
BsmFI GGGAC 2 cut(s) 533, 549
BsmI GAATGC 1 cut(s) 744
BsnI GGCC 6 cut(s) 431, 496, 638, 865, 929, 1117
BsoBI CYCGRG 1 cut(s) 823
Bsp1286I GDGCHC 2 cut(s) 567, 918
Bsp143I GATC 6 cut(s) 896, 922, 946, 1042, 1157, 1273
Bsp19I CCATGG 2 cut(s) 426, 613
BspACI CCGC 5 cut(s) 227, 266, 272, 275, 889
BspANI GGCC 6 cut(s) 431, 496, 638, 865, 929, 1117
BspCNI CTCAG 2 cut(s) 360, 1045
BspDI ATCGAT 1 cut(s) 999
BspFNI CGCG 1 cut(s) 569
BspLI GGNNCC 4 cut(s) 432, 510, 866, 1236
BspPI GGATC 3 cut(s) 904, 1165, 1268
BsrI ACTGG 1 cut(s) 650
BssECI CCNNGG 4 cut(s) 426, 613, 824, 1100
BssMI GATC 6 cut(s) 896, 922, 946, 1042, 1157, 1273
BssSI CACGAG 1 cut(s) 539
BssT1I CCWWGG 2 cut(s) 426, 613
Bst2BI CACGAG 1 cut(s) 539
Bst2UI CCWGG 4 cut(s) 116, 635, 834, 1101
Bst4CI ACNGT 3 cut(s) 288, 338, 458
Bst6I CTCTTC 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 567
BstDEI CTNAG 3 cut(s) 347, 806, 1053
BstDSI CCRYGG 2 cut(s) 426, 613
BstENI CCTNNNNNAGG 1 cut(s) 837
BstF5I GGATG 5 cut(s) 111, 422, 673, 1096, 1118
BstFNI CGCG 1 cut(s) 569
BstH2I RGCGCY 1 cut(s) 847
BstHHI GCGC 2 cut(s) 571, 846
BstKTI GATC 6 cut(s) 899, 925, 949, 1045, 1160, 1276
BstMAI GTCTC 2 cut(s) 350, 946
BstMBI GATC 6 cut(s) 896, 922, 946, 1042, 1157, 1273
BstMCI CGRYCG 1 cut(s) 236
BstMWI GCNNNNNNNGC 5 cut(s) 24, 274, 348, 428, 807
BstNI CCWGG 4 cut(s) 116, 635, 834, 1101
BstNSI RCATGY 1 cut(s) 1033
BstSCI CCNGG 4 cut(s) 114, 633, 832, 1099
BstSLI GKGCMC 2 cut(s) 567, 918
BstUI CGCG 1 cut(s) 569
BstV1I GCAGC 6 cut(s) 17, 30, 33, 36, 604, 1248
BstV2I GAAGAC 1 cut(s) 601
BstX2I RGATCY 2 cut(s) 896, 922
BstYI RGATCY 2 cut(s) 896, 922
Bsu15I ATCGAT 1 cut(s) 999
BsuRI GGCC 6 cut(s) 431, 496, 638, 865, 929, 1117
BsuTUI ATCGAT 1 cut(s) 999
BtgI CCRYGG 2 cut(s) 426, 613
BtsCI GGATG 5 cut(s) 111, 422, 673, 1096, 1118
BtsI GCAGTG 4 cut(s) 34, 695, 1169, 1256
BtsIMutI CAGTG 4 cut(s) 34, 695, 1169, 1256
Cac8I GCNNGC 1 cut(s) 567
CfoI GCGC 2 cut(s) 571, 846
Cfr13I GGNCC 4 cut(s) 430, 494, 864, 1091
ClaI ATCGAT 1 cut(s) 999
Csp6I GTAC 3 cut(s) 284, 386, 1036
CspCI CAANNNNNGTGG 2 cut(s) 971, 1006
CviAII CATG 6 cut(s) 394, 427, 478, 614, 1030, 1068
CviQI GTAC 3 cut(s) 284, 386, 1036
DdeI CTNAG 3 cut(s) 347, 806, 1053
DpnI GATC 6 cut(s) 898, 924, 948, 1044, 1159, 1275
DpnII GATC 6 cut(s) 896, 922, 946, 1042, 1157, 1273
DrdI GACNNNNNNGTC 1 cut(s) 543
DseDI GACNNNNNNGTC 1 cut(s) 543
EaeI YGGCCR 1 cut(s) 927
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
EciI GGCGGA 2 cut(s) 216, 904
Eco130I CCWWGG 2 cut(s) 426, 613
Eco47I GGWCC 1 cut(s) 1091
Eco88I CYCGRG 1 cut(s) 823
EcoNI CCTNNNNNAGG 1 cut(s) 837
EcoO109I RGGNCCY 1 cut(s) 494
EcoRI GAATTC 1 cut(s) 1146
EcoRII CCWGG 4 cut(s) 114, 633, 832, 1099
EcoT14I CCWWGG 2 cut(s) 426, 613
ErhI CCWWGG 2 cut(s) 426, 613
FaeI CATG 6 cut(s) 397, 430, 481, 617, 1033, 1071
FaqI GGGAC 2 cut(s) 533, 549
FatI CATG 6 cut(s) 393, 426, 477, 613, 1029, 1067
Fnu4HI GCNGC 7 cut(s) 6, 19, 22, 25, 275, 593, 1262
FokI GGATG 5 cut(s) 98, 409, 680, 1083, 1125
Fsp4HI GCNGC 7 cut(s) 6, 19, 22, 25, 275, 593, 1262
FspBI CTAG 3 cut(s) 33, 354, 1279
GlaI GCGC 2 cut(s) 570, 845
GluI GCNGC 7 cut(s) 6, 19, 22, 25, 275, 593, 1262
GsuI CTGGAG 1 cut(s) 1253
HaeII RGCGCY 1 cut(s) 847
HaeIII GGCC 6 cut(s) 431, 496, 638, 865, 929, 1117
HhaI GCGC 2 cut(s) 571, 846
Hin1II CATG 6 cut(s) 397, 430, 481, 617, 1033, 1071
Hin6I GCGC 2 cut(s) 569, 844
HinP1I GCGC 2 cut(s) 569, 844
HincII GTYRAC 1 cut(s) 1027
HindII GTYRAC 1 cut(s) 1027
HindIII AAGCTT 1 cut(s) 799
HinfI GANTC 3 cut(s) 443, 587, 737
HphI GGTGA 1 cut(s) 201
Hpy166II GTNNAC 7 cut(s) 388, 565, 643, 787, 916, 934, 1027
Hpy188I TCNGA 6 cut(s) 465, 736, 946, 951, 1054, 1157
Hpy188III TCNNGA 4 cut(s) 133, 484, 763, 1232
Hpy8I GTNNAC 7 cut(s) 388, 565, 643, 787, 916, 934, 1027
Hpy99I CGWCG 3 cut(s) 404, 681, 821
HpyAV CCTTC 1 cut(s) 585
HpyCH4III ACNGT 3 cut(s) 288, 338, 458
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 9 cut(s) 18, 76, 308, 565, 671, 695, 744, 916, 1167
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 274, 348, 428, 807
HpyF3I CTNAG 3 cut(s) 347, 806, 1053
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 6 cut(s) 397, 430, 481, 617, 1033, 1071
HspAI GCGC 2 cut(s) 569, 844
Kzo9I GATC 6 cut(s) 896, 922, 946, 1042, 1157, 1273
LmnI GCTCC 3 cut(s) 514, 1059, 1234
Lsp1109I GCAGC 6 cut(s) 17, 30, 33, 36, 604, 1248
LweI GCATC 4 cut(s) 120, 170, 431, 658
MaeI CTAG 3 cut(s) 33, 354, 1279
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 5 cut(s) 102, 137, 148, 408, 1242
MalI GATC 6 cut(s) 898, 924, 948, 1044, 1159, 1275
MboI GATC 6 cut(s) 896, 922, 946, 1042, 1157, 1273
MboII GAAGA 3 cut(s) 76, 601, 633
MflI RGATCY 2 cut(s) 896, 922
MhlI GDGCHC 2 cut(s) 567, 918
MmeI TCCRAC 2 cut(s) 645, 1040
MseI TTAA 7 cut(s) 177, 189, 303, 330, 582, 627, 756
MspR9I CCNGG 4 cut(s) 116, 635, 834, 1101
Mva1269I GAATGC 1 cut(s) 744
MvaI CCWGG 4 cut(s) 116, 635, 834, 1101
MvnI CGCG 1 cut(s) 569
MwoI GCNNNNNNNGC 5 cut(s) 24, 274, 348, 428, 807
NcoI CCATGG 2 cut(s) 426, 613
NdeII GATC 6 cut(s) 896, 922, 946, 1042, 1157, 1273
NlaIII CATG 6 cut(s) 397, 430, 481, 617, 1033, 1071
NlaIV GGNNCC 4 cut(s) 432, 510, 866, 1236
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 2 cut(s) 102, 148
NspI RCATGY 1 cut(s) 1033
PciI ACATGT 1 cut(s) 1029
PctI GAATGC 1 cut(s) 744
PfeI GAWTC 3 cut(s) 443, 587, 737
PkrI GCNGC 7 cut(s) 7, 20, 23, 26, 276, 594, 1263
PscI ACATGT 1 cut(s) 1029
PshBI ATTAAT 3 cut(s) 177, 303, 627
PsiI TTATAA 1 cut(s) 453
Psp6I CCWGG 4 cut(s) 114, 633, 832, 1099
PspGI CCWGG 4 cut(s) 114, 633, 832, 1099
PspN4I GGNNCC 4 cut(s) 432, 510, 866, 1236
PspPI GGNCC 4 cut(s) 430, 494, 864, 1091
PsuI RGATCY 2 cut(s) 896, 922
RsaI GTAC 3 cut(s) 285, 387, 1037
RsaNI GTAC 3 cut(s) 284, 386, 1036
SaqAI TTAA 7 cut(s) 177, 189, 303, 330, 582, 627, 756
SatI GCNGC 7 cut(s) 6, 19, 22, 25, 275, 593, 1262
Sau3AI GATC 6 cut(s) 896, 922, 946, 1042, 1157, 1273
Sau96I GGNCC 4 cut(s) 430, 494, 864, 1091
ScrFI CCNGG 4 cut(s) 116, 635, 834, 1101
SduI GDGCHC 2 cut(s) 567, 918
SfaNI GCATC 4 cut(s) 120, 170, 431, 658
SinI GGWCC 1 cut(s) 1091
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
SpeI ACTAGT 1 cut(s) 353
SsiI CCGC 5 cut(s) 227, 266, 272, 275, 889
SspMI CTAG 3 cut(s) 33, 354, 1279
StyD4I CCNGG 4 cut(s) 114, 633, 832, 1099
StyI CCWWGG 2 cut(s) 426, 613
TaaI ACNGT 3 cut(s) 288, 338, 458
TaiI ACGT 1 cut(s) 72
TaqI TCGA 5 cut(s) 232, 619, 816, 838, 999
TaqII GACCGA 1 cut(s) 250
TatI WGTACW 1 cut(s) 385
TauI GCSGC 1 cut(s) 277
TfiI GAWTC 3 cut(s) 443, 587, 737
Tru1I TTAA 7 cut(s) 177, 189, 303, 330, 582, 627, 756
Tru9I TTAA 7 cut(s) 177, 189, 303, 330, 582, 627, 756
TscAI CASTG 4 cut(s) 34, 702, 1169, 1263
TseFI GTSAC 2 cut(s) 102, 148
TseI GCWGC 6 cut(s) 5, 18, 21, 24, 592, 1261
Tsp45I GTSAC 2 cut(s) 102, 148
TspDTI ATGAA 3 cut(s) 466, 600, 1179
TspRI CASTG 4 cut(s) 34, 702, 1169, 1263
VneI GTGCAC 2 cut(s) 563, 914
VpaK11BI GGWCC 1 cut(s) 1091
VspI ATTAAT 3 cut(s) 177, 303, 627
XagI CCTNNNNNAGG 1 cut(s) 837
XapI RAATTY 3 cut(s) 524, 1106, 1146
XceI RCATGY 1 cut(s) 1033
XspI CTAG 3 cut(s) 33, 354, 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.