RchiOBHm_Chr3g0495591

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0008998
Physical Location & Seq
Forward (+)
45286855 .. 45288292
1438 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45798

Sequence Viewer

Length: 1335 bp
ATGTATCAGTTGGCTCCTTCTCGTACACATATAATTGTTTCAATCAGATCGATATTAATGATGGCAGTCATGGCACCAATATTCTGCAGCAGCAGTAGTACTAGTGGGCAAAATCATCGGGCGTTTCCATTCAATCACGTCCTGCATTTCCTCTTCTTTGTTATTGGTTTGTCACTCGGCATCCTAGTTACCTTATACTTCCAGAGTTACTCATTACTCACTTTTCAAGCATCCCTCAACTCCATTAATAATTTTATTACCATTCCACTAAAACCATCACCACCGAAATCACCAACTCCAACTCCACCTCCACCTCCACCACCGCCAGTTCTGGTACCGTCTTTCTCTACAATTAATGAAACATCATCATCAGTATCTGTTCATTACAGTAGCAGTACTCCCAGTACCAGTGTTTCTTTGAAAGAACAAAAGCTGTTAGTACACAACATGAGCGATGAGGAGTTACTTTGGAGAGCATCCATGGCCCCTCAAGTTGATTCGCCTTGCAACTCTGTTCCGAAAGTGGCTTTCATGTTCTTGACAAAAGGGCCACTACCTTTGGCTCCCTTATGGGACAAATTTTTCAAGGGACACGAGGGTCTATACTCCATCTTTGTGCACGCGCACCCTTCTTTCAATGAATCGCAGCCTCAAACTTCGGTCTTCCATGGTCGAAGAATTCATAGCCAGGCCGTTTATTGGGGAACTTCCTCGATGTTGGATGCAGAGCGACGGCTATTAGCCAATGCACTGCTTGACTTGTCCAATCAAAGATTTGTATTGCTTTCCGAATCTTGCATTCCCTTGTTTAACTTCACGACAACTTATGACTACCTTATAAACTCAAATCAAAGCTTTCAAAGCGTATACGATGACCCGAGGAAGCCTGGTCGAGGGCGCTACAACCCTCAAATGTGGCCACAAATAAACATCACAGATTGGCGGAAAGGATCTCAATGGTTTGAAGTGCACCGAGACCTGGCCGTCCACATTGTCTCCGATCGGAAATACTACCCCATCTTTCAACAATACTGCCACCCTCCTTGTTATATCGATGAGCATTATATTCCAACATTCGTCAACATGTTGTATGGTGATCTCAACTCTGAGAGAAGCATTACATGGGTGGATTGGTCAAGGGGTGGTGCTCATCCTCGGAAATTTGGATGGCCTGATATTACAGATGAGTTTTTTAATGGAATTCGGTTCGGATCGCACTGTAAATACAACGGCAATACAACTTCAATATGTTTCTTATTTGGTAGAAAGTTTTTGCCTAATGCTCTGGAGCCTTTGTTACGAGTTGCTCCACTGCTGCTTGGCTTTGATACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

444

Amino Acids

50.5

Weight (kDa)

8.73

Isoelectric Point (pI)

47.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 175 - 401 4.3e-82 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 839
AasI GACNNNNNNGTC 2 cut(s) 597, 983
Acc65I GGTACC 1 cut(s) 334
AccB1I GGYRCC 2 cut(s) 73, 334
AccI GTMKAC 1 cut(s) 867
AccII CGCG 1 cut(s) 623
AciI CCGC 2 cut(s) 323, 943
AclWI GGATC 2 cut(s) 958, 1219
AcoI YGGCCR 2 cut(s) 917, 981
AcsI RAATTY 4 cut(s) 578, 678, 1160, 1200
AfaI GTAC 6 cut(s) 25, 100, 336, 397, 406, 441
AfiI CCNNNNNNNGG 3 cut(s) 699, 893, 979
AflIII ACRYGT 1 cut(s) 1083
AhlI ACTAGT 1 cut(s) 101
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 3 cut(s) 687, 886, 978
AluBI AGCT 2 cut(s) 433, 855
AluI AGCT 2 cut(s) 433, 855
Alw21I GWGCWC 3 cut(s) 621, 972, 1150
Alw26I GTCTC 2 cut(s) 969, 1000
Alw44I GTGCAC 2 cut(s) 617, 968
AlwI GGATC 2 cut(s) 958, 1219
AlwNI CAGNNNCTG 1 cut(s) 377
Ama87I CYCGRG 1 cut(s) 877
AoxI GGCC 6 cut(s) 483, 548, 690, 917, 981, 1169
ApaLI GTGCAC 2 cut(s) 617, 968
ApeKI GCWGC 4 cut(s) 87, 90, 646, 1315
ApoI RAATTY 4 cut(s) 578, 678, 1160, 1200
AseI ATTAAT 3 cut(s) 56, 246, 354
Asp718I GGTACC 1 cut(s) 334
AspLEI GCGC 2 cut(s) 625, 900
AspS9I GGNCC 2 cut(s) 484, 548
AsuHPI GGTGA 3 cut(s) 270, 282, 1106
AvaI CYCGRG 1 cut(s) 877
BaeGI GKGCMC 2 cut(s) 621, 972
BalI TGGCCA 1 cut(s) 919
BanI GGYRCC 2 cut(s) 73, 334
BauI CACGAG 1 cut(s) 593
BbsI GAAGAC 1 cut(s) 655
Bbv12I GWGCWC 3 cut(s) 621, 972, 1150
BbvI GCAGC 4 cut(s) 99, 102, 658, 1302
BccI CCATC 5 cut(s) 55, 283, 617, 1025, 1161
BceAI ACGGC 4 cut(s) 677, 749, 968, 1246
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 3 cut(s) 689, 888, 980
BcoDI GTCTC 2 cut(s) 969, 1000
BcuI ACTAGT 1 cut(s) 101
BfaI CTAG 3 cut(s) 102, 185, 1333
BfmI CTRYAG 1 cut(s) 85
BfoI RGCGCY 1 cut(s) 901
BisI GCNGC 4 cut(s) 88, 91, 647, 1316
BlsI GCNGC 4 cut(s) 89, 92, 648, 1317
BmcAI AGTACT 2 cut(s) 100, 397
Bme1390I CCNGG 3 cut(s) 689, 888, 980
BmeT110I CYCGRG 1 cut(s) 877
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 2 cut(s) 484, 548
BmiI GGNNCC 6 cut(s) 15, 75, 336, 486, 564, 1290
BmrFI CCNGG 3 cut(s) 689, 888, 980
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 4 cut(s) 189, 239, 485, 712
BmuI ACTGGG 1 cut(s) 396
BpiI GAAGAC 1 cut(s) 655
BpmI CTGGAG 1 cut(s) 1307
BpuEI CTTGAG 1 cut(s) 474
Bsa29I ATCGAT 2 cut(s) 50, 1053
BsaI GGTCTC 1 cut(s) 969
BsaJI CCNNGG 4 cut(s) 480, 667, 878, 1154
BsaXI ACNNNNNCTCC 8 cut(s) 286, 292, 316, 322, 980, 1010, 1280, 1310
Bsc4I CCNNNNNNNGG 3 cut(s) 699, 893, 979
Bse1I ACTGG 3 cut(s) 326, 402, 408
BseBI CCWGG 3 cut(s) 689, 888, 980
BseCI ATCGAT 2 cut(s) 50, 1053
BseDI CCNNGG 4 cut(s) 480, 667, 878, 1154
BseGI GGATG 6 cut(s) 180, 230, 476, 727, 1150, 1172
BseLI CCNNNNNNNGG 3 cut(s) 699, 893, 979
BseMII CTCAG 1 cut(s) 1098
BseNI ACTGG 3 cut(s) 326, 402, 408
BseRI GAGGAG 1 cut(s) 473
BseSI GKGCMC 2 cut(s) 621, 972
BseXI GCAGC 4 cut(s) 99, 102, 658, 1302
Bsh1236I CGCG 1 cut(s) 623
Bsh1285I CGRYCG 1 cut(s) 1003
BshFI GGCC 6 cut(s) 485, 550, 692, 919, 983, 1171
BshNI GGYRCC 2 cut(s) 73, 334
BshVI ATCGAT 2 cut(s) 50, 1053
BsiEI CGRYCG 1 cut(s) 1003
BsiHKAI GWGCWC 3 cut(s) 621, 972, 1150
BsiHKCI CYCGRG 1 cut(s) 877
BslFI GGGAC 2 cut(s) 587, 603
BslI CCNNNNNNNGG 3 cut(s) 699, 893, 979
BsmAI GTCTC 2 cut(s) 969, 1000
BsmFI GGGAC 2 cut(s) 587, 603
BsmI GAATGC 1 cut(s) 798
BsnI GGCC 6 cut(s) 485, 550, 692, 919, 983, 1171
Bso31I GGTCTC 1 cut(s) 969
BsoBI CYCGRG 1 cut(s) 877
Bsp1286I GDGCHC 3 cut(s) 621, 972, 1150
Bsp143I GATC 5 cut(s) 47, 950, 1000, 1096, 1211
Bsp19I CCATGG 2 cut(s) 480, 667
BspACI CCGC 2 cut(s) 323, 943
BspANI GGCC 6 cut(s) 485, 550, 692, 919, 983, 1171
BspCNI CTCAG 1 cut(s) 1099
BspDI ATCGAT 2 cut(s) 50, 1053
BspFNI CGCG 1 cut(s) 623
BspLI GGNNCC 6 cut(s) 15, 75, 336, 486, 564, 1290
BspMAI CTGCAG 1 cut(s) 89
BspPI GGATC 2 cut(s) 958, 1219
BspT107I GGYRCC 2 cut(s) 73, 334
BspTNI GGTCTC 1 cut(s) 969
BsrI ACTGG 3 cut(s) 326, 402, 408
BssECI CCNNGG 4 cut(s) 480, 667, 878, 1154
BssMI GATC 5 cut(s) 47, 950, 1000, 1096, 1211
BssNAI GTATAC 1 cut(s) 868
BssSI CACGAG 1 cut(s) 593
BssT1I CCWWGG 2 cut(s) 480, 667
Bst1107I GTATAC 1 cut(s) 868
Bst2BI CACGAG 1 cut(s) 593
Bst2UI CCWGG 3 cut(s) 689, 888, 980
Bst4CI ACNGT 3 cut(s) 339, 389, 1220
Bst6I CTCTTC 1 cut(s) 158
BstC8I GCNNGC 1 cut(s) 621
BstDEI CTNAG 1 cut(s) 1107
BstDSI CCRYGG 2 cut(s) 480, 667
BstENI CCTNNNNNAGG 1 cut(s) 891
BstF5I GGATG 6 cut(s) 180, 230, 476, 727, 1150, 1172
BstFNI CGCG 1 cut(s) 623
BstH2I RGCGCY 1 cut(s) 901
BstHHI GCGC 2 cut(s) 625, 900
BstKTI GATC 5 cut(s) 50, 953, 1003, 1099, 1214
BstMAI GTCTC 2 cut(s) 969, 1000
BstMBI GATC 5 cut(s) 47, 950, 1000, 1096, 1211
BstMCI CGRYCG 1 cut(s) 1003
BstMWI GCNNNNNNNGC 3 cut(s) 71, 482, 861
BstNI CCWGG 3 cut(s) 689, 888, 980
BstNSI RCATGY 1 cut(s) 1087
BstSCI CCNGG 3 cut(s) 687, 886, 978
BstSFI CTRYAG 1 cut(s) 85
BstSLI GKGCMC 2 cut(s) 621, 972
BstUI CGCG 1 cut(s) 623
BstV1I GCAGC 4 cut(s) 99, 102, 658, 1302
BstV2I GAAGAC 1 cut(s) 655
BstX2I RGATCY 1 cut(s) 950
BstYI RGATCY 1 cut(s) 950
BstZ17I GTATAC 1 cut(s) 868
Bsu15I ATCGAT 2 cut(s) 50, 1053
BsuRI GGCC 6 cut(s) 485, 550, 692, 919, 983, 1171
BsuTUI ATCGAT 2 cut(s) 50, 1053
BtgI CCRYGG 2 cut(s) 480, 667
BtgZI GCGATG 1 cut(s) 468
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 6 cut(s) 180, 230, 476, 727, 1150, 1172
BtsI GCAGTG 2 cut(s) 749, 1310
BtsIMutI CAGTG 4 cut(s) 415, 749, 1216, 1310
Cac8I GCNNGC 1 cut(s) 621
CaiI CAGNNNCTG 1 cut(s) 377
CfoI GCGC 2 cut(s) 625, 900
Cfr13I GGNCC 2 cut(s) 484, 548
ClaI ATCGAT 2 cut(s) 50, 1053
Csp6I GTAC 6 cut(s) 24, 99, 335, 396, 405, 440
CspCI CAANNNNNGTGG 4 cut(s) 540, 575, 1025, 1060
CviAII CATG 7 cut(s) 70, 448, 481, 532, 668, 1084, 1122
CviQI GTAC 6 cut(s) 24, 99, 335, 396, 405, 440
DdeI CTNAG 1 cut(s) 1107
DpnI GATC 5 cut(s) 49, 952, 1002, 1098, 1213
DpnII GATC 5 cut(s) 47, 950, 1000, 1096, 1211
DrdI GACNNNNNNGTC 2 cut(s) 597, 983
DseDI GACNNNNNNGTC 2 cut(s) 597, 983
EaeI YGGCCR 2 cut(s) 917, 981
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
EciI GGCGGA 1 cut(s) 958
Eco130I CCWWGG 2 cut(s) 480, 667
Eco31I GGTCTC 1 cut(s) 969
Eco88I CYCGRG 1 cut(s) 877
EcoNI CCTNNNNNAGG 1 cut(s) 891
EcoRI GAATTC 2 cut(s) 678, 1200
EcoRII CCWGG 3 cut(s) 687, 886, 978
EcoT14I CCWWGG 2 cut(s) 480, 667
ErhI CCWWGG 2 cut(s) 480, 667
FaeI CATG 7 cut(s) 73, 451, 484, 535, 671, 1087, 1125
FaqI GGGAC 2 cut(s) 587, 603
FatI CATG 7 cut(s) 69, 447, 480, 531, 667, 1083, 1121
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 4 cut(s) 88, 91, 647, 1316
FokI GGATG 6 cut(s) 167, 217, 463, 734, 1137, 1179
Fsp4HI GCNGC 4 cut(s) 88, 91, 647, 1316
FspBI CTAG 3 cut(s) 102, 185, 1333
GlaI GCGC 2 cut(s) 624, 899
GluI GCNGC 4 cut(s) 88, 91, 647, 1316
GsuI CTGGAG 1 cut(s) 1307
HaeII RGCGCY 1 cut(s) 901
HaeIII GGCC 6 cut(s) 485, 550, 692, 919, 983, 1171
HhaI GCGC 2 cut(s) 625, 900
Hin1II CATG 7 cut(s) 73, 451, 484, 535, 671, 1087, 1125
Hin6I GCGC 2 cut(s) 623, 898
HinP1I GCGC 2 cut(s) 623, 898
HincII GTYRAC 1 cut(s) 1081
HindII GTYRAC 1 cut(s) 1081
HindIII AAGCTT 1 cut(s) 853
HinfI GANTC 3 cut(s) 497, 641, 791
HphI GGTGA 3 cut(s) 270, 282, 1106
Hpy166II GTNNAC 7 cut(s) 26, 442, 619, 868, 970, 988, 1081
Hpy188I TCNGA 8 cut(s) 47, 519, 790, 1000, 1005, 1108, 1158, 1211
Hpy188III TCNNGA 4 cut(s) 202, 538, 817, 1286
Hpy8I GTNNAC 7 cut(s) 26, 442, 619, 868, 970, 988, 1081
Hpy99I CGWCG 1 cut(s) 735
HpyAV CCTTC 2 cut(s) 27, 639
HpyCH4III ACNGT 3 cut(s) 339, 389, 1220
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 8 cut(s) 87, 145, 507, 619, 725, 749, 798, 970
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 482, 861
HpyF3I CTNAG 1 cut(s) 1107
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 7 cut(s) 73, 451, 484, 535, 671, 1087, 1125
HspAI GCGC 2 cut(s) 623, 898
KpnI GGTACC 1 cut(s) 338
Kzo9I GATC 5 cut(s) 47, 950, 1000, 1096, 1211
LmnI GCTCC 4 cut(s) 19, 568, 1288, 1312
Lsp1109I GCAGC 4 cut(s) 99, 102, 658, 1302
LweI GCATC 4 cut(s) 189, 239, 485, 712
MaeI CTAG 3 cut(s) 102, 185, 1333
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 5 cut(s) 171, 187, 206, 462, 1296
MalI GATC 5 cut(s) 49, 952, 1002, 1098, 1213
MboI GATC 5 cut(s) 47, 950, 1000, 1096, 1211
MboII GAAGA 3 cut(s) 145, 655, 687
MflI RGATCY 1 cut(s) 950
MhlI GDGCHC 3 cut(s) 621, 972, 1150
MlsI TGGCCA 1 cut(s) 919
MluCI AATT 7 cut(s) 33, 250, 351, 578, 678, 1160, 1200
MluNI TGGCCA 1 cut(s) 919
MmeI TCCRAC 3 cut(s) 323, 699, 1094
Mox20I TGGCCA 1 cut(s) 919
MscI TGGCCA 1 cut(s) 919
MseI TTAA 5 cut(s) 56, 246, 354, 810, 1194
MslI CAYNNNNRTG 1 cut(s) 614
Msp20I TGGCCA 1 cut(s) 919
MspR9I CCNGG 3 cut(s) 689, 888, 980
Mva1269I GAATGC 1 cut(s) 798
MvaI CCWGG 3 cut(s) 689, 888, 980
MvnI CGCG 1 cut(s) 623
MwoI GCNNNNNNNGC 3 cut(s) 71, 482, 861
NcoI CCATGG 2 cut(s) 480, 667
NdeII GATC 5 cut(s) 47, 950, 1000, 1096, 1211
NlaIII CATG 7 cut(s) 73, 451, 484, 535, 671, 1087, 1125
NlaIV GGNNCC 6 cut(s) 15, 75, 336, 486, 564, 1290
NmeAIII GCCGAG 1 cut(s) 156
NmuCI GTSAC 1 cut(s) 171
NspI RCATGY 1 cut(s) 1087
PciI ACATGT 1 cut(s) 1083
PctI GAATGC 1 cut(s) 798
PfeI GAWTC 3 cut(s) 497, 641, 791
PkrI GCNGC 4 cut(s) 89, 92, 648, 1317
Ple19I CGATCG 1 cut(s) 1003
PscI ACATGT 1 cut(s) 1083
PshBI ATTAAT 3 cut(s) 56, 246, 354
PsiI TTATAA 1 cut(s) 839
Psp6I CCWGG 3 cut(s) 687, 886, 978
PspGI CCWGG 3 cut(s) 687, 886, 978
PspN4I GGNNCC 6 cut(s) 15, 75, 336, 486, 564, 1290
PspPI GGNCC 2 cut(s) 484, 548
PstI CTGCAG 1 cut(s) 89
PstNI CAGNNNCTG 1 cut(s) 377
PsuI RGATCY 1 cut(s) 950
PvuI CGATCG 1 cut(s) 1003
RsaI GTAC 6 cut(s) 25, 100, 336, 397, 406, 441
RsaNI GTAC 6 cut(s) 24, 99, 335, 396, 405, 440
RseI CAYNNNNRTG 1 cut(s) 614
SaqAI TTAA 5 cut(s) 56, 246, 354, 810, 1194
SatI GCNGC 4 cut(s) 88, 91, 647, 1316
Sau3AI GATC 5 cut(s) 47, 950, 1000, 1096, 1211
Sau96I GGNCC 2 cut(s) 484, 548
ScaI AGTACT 2 cut(s) 100, 397
ScrFI CCNGG 3 cut(s) 689, 888, 980
SduI GDGCHC 3 cut(s) 621, 972, 1150
SfaNI GCATC 4 cut(s) 189, 239, 485, 712
SfcI CTRYAG 1 cut(s) 85
SmiMI CAYNNNNRTG 1 cut(s) 614
SmlI CTYRAG 1 cut(s) 489
SmoI CTYRAG 1 cut(s) 489
SpeI ACTAGT 1 cut(s) 101
Sse9I AATT 7 cut(s) 33, 250, 351, 578, 678, 1160, 1200
SsiI CCGC 2 cut(s) 323, 943
SspI AATATT 1 cut(s) 81
SspMI CTAG 3 cut(s) 102, 185, 1333
StyD4I CCNGG 3 cut(s) 687, 886, 978
StyI CCWWGG 2 cut(s) 480, 667
TaaI ACNGT 3 cut(s) 339, 389, 1220
TaiI ACGT 1 cut(s) 141
TaqI TCGA 5 cut(s) 50, 673, 713, 892, 1053
TaqII GACCGA 1 cut(s) 649
TasI AATT 7 cut(s) 33, 250, 351, 578, 678, 1160, 1200
TatI WGTACW 3 cut(s) 98, 395, 439
TfiI GAWTC 3 cut(s) 497, 641, 791
Tru1I TTAA 5 cut(s) 56, 246, 354, 810, 1194
Tru9I TTAA 5 cut(s) 56, 246, 354, 810, 1194
TscAI CASTG 4 cut(s) 415, 756, 1223, 1317
TseFI GTSAC 1 cut(s) 171
TseI GCWGC 4 cut(s) 87, 90, 646, 1315
Tsp45I GTSAC 1 cut(s) 171
TspDTI ATGAA 5 cut(s) 371, 372, 520, 654, 671
TspRI CASTG 4 cut(s) 415, 756, 1223, 1317
VneI GTGCAC 2 cut(s) 617, 968
VspI ATTAAT 3 cut(s) 56, 246, 354
XagI CCTNNNNNAGG 1 cut(s) 891
XapI RAATTY 4 cut(s) 578, 678, 1160, 1200
XceI RCATGY 1 cut(s) 1087
XmiI GTMKAC 1 cut(s) 867
XspI CTAG 3 cut(s) 102, 185, 1333
ZrmI AGTACT 2 cut(s) 100, 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.