RLG00000022608

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008998
Physical Location & Seq
Reverse (-)
5178458 .. 5179838
1381 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022608

Sequence Viewer

Length: 1278 bp
ATGGCACCAATATTCTGCAGCAGCAGTAGTACTAGTGGGCAAAATCATCGGGCGTTTCCATTCAATCACGTCCTGCATTTCCTCTTCTTTGTTATTGGTTTGTCACTCGGCATCCTAGTTACCTTATACTTCCAGAGTTACTCATTACTCACTTTTCAAGCATCCCTCAACTCCATTAATAATTTTATTAACATTCCACTAAAACCATCACCCCCATCTCCACCTCCGCCTCCACCGAAATCACCAACTCCACCTCCATCTCCACCACCACCAGTTCTGGTACCGTCTTTCTCTACAATTAATGAAGCATCATCATCAGTATCTGTTAATTACAGTACCAGTCCCAGTACTAGTGTTTCTTTGAAAGAACAAAAGCTGTTGGTACACAACATGAGCGATGAGGAGTTACTTGGGAGAGCATCCATGGCCCCTCTAGTTGATTCGCCTTACAACTCTGTTCCGAAAGTGGCTTTCATGTTCTTGACAAAAGGGCCACTACCTTTGGCTCCCTTATGGGACAAATTTTTCAAGGGACACGAGGGTCTATACTCCATCTATGTGCACGCGCACCCTTCTTTCAATGAATCGCAGCCTCAAACTTCGGTCTTCTATGGTCGAAGAATTCATAGCCAGGCCGTTTATTGGGGAACTTCCTCGATGTTGGATGCAGAGCGACGGCTATTAGCGAATGCACTGCTTGACTTGTCCAATCAGAGATTTGTATTGCTTTCCGAATCTTGCATTCCCTTGTTTAACTTCACGACAACTTATGACTACCTTATAAACTCAAATCAAAGCTTTCTAAGCGTATATGATGACCCGAGGAAGCCTGGTCGAGGGCGCTACAACCCTCAAATGTGGCCACAAATAAACATCACAGATTGGCGGAAAGGATCTCAATGGTTTGAAGTGCACCGAGACCTGGCCGTCCACATTGTCTCCGATCGGAAATACTACCCCATCTTTCAACAATACTGCCACCCTCCTTGTTATATCGATGAGCATTATATTCCAACATTCGTCAACATGTTGTATGCTGATCTCAACTCTGAGAGAAGCATTACATGGGTGGATTGGTCAAGGGGTGGTGCTCATCCTCGGAAATTTGGATGGCCTGATATTACAGATGAGTTTTTTAATGGAATTCGGTTCGGATCGCACTGTAAATACAACGGCAATACATCTTCAATATGTTTCTTATTTGGTAGAAAGTTTTTGCCTAATGCTCTGGAGCCTTTGTTACGAGTTGCTCCACTGCTGCTTGGCTTTGATACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

426

Amino Acids

48.16

Weight (kDa)

8.42

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 156 - 382 3.2e-83 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 782
AasI GACNNNNNNGTC 2 cut(s) 540, 926
Acc65I GGTACC 1 cut(s) 280
AccB1I GGYRCC 2 cut(s) 4, 280
AccII CGCG 1 cut(s) 566
AciI CCGC 2 cut(s) 227, 886
AclWI GGATC 2 cut(s) 901, 1162
AcoI YGGCCR 2 cut(s) 860, 924
AcsI RAATTY 4 cut(s) 521, 621, 1103, 1143
AfaI GTAC 5 cut(s) 31, 282, 337, 349, 384
AfiI CCNNNNNNNGG 3 cut(s) 642, 836, 922
AflIII ACRYGT 1 cut(s) 1026
AgsI TTSAA 8 cut(s) 64, 158, 364, 529, 580, 908, 968, 1188
AhlI ACTAGT 2 cut(s) 32, 350
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 3 cut(s) 630, 829, 921
AluBI AGCT 2 cut(s) 376, 798
AluI AGCT 2 cut(s) 376, 798
Alw21I GWGCWC 3 cut(s) 564, 915, 1093
Alw26I GTCTC 2 cut(s) 912, 943
Alw44I GTGCAC 2 cut(s) 560, 911
AlwI GGATC 2 cut(s) 901, 1162
AlwNI CAGNNNCTG 1 cut(s) 323
Ama87I CYCGRG 1 cut(s) 820
AoxI GGCC 6 cut(s) 426, 491, 633, 860, 924, 1112
ApaLI GTGCAC 2 cut(s) 560, 911
ApeKI GCWGC 4 cut(s) 18, 21, 589, 1258
ApoI RAATTY 4 cut(s) 521, 621, 1103, 1143
AseI ATTAAT 2 cut(s) 177, 300
Asp718I GGTACC 1 cut(s) 280
AspLEI GCGC 2 cut(s) 568, 843
AspS9I GGNCC 2 cut(s) 427, 491
AsuHPI GGTGA 2 cut(s) 201, 234
AvaI CYCGRG 1 cut(s) 820
BaeGI GKGCMC 2 cut(s) 564, 915
BalI TGGCCA 1 cut(s) 862
BanI GGYRCC 2 cut(s) 4, 280
BauI CACGAG 1 cut(s) 536
BbsI GAAGAC 1 cut(s) 598
Bbv12I GWGCWC 3 cut(s) 564, 915, 1093
BbvI GCAGC 4 cut(s) 30, 33, 601, 1245
BccI CCATC 6 cut(s) 214, 223, 265, 560, 968, 1104
BceAI ACGGC 4 cut(s) 620, 692, 911, 1189
BcgI CGANNNNNNTGC 4 cut(s) 29, 63, 676, 710
BciT130I CCWGG 3 cut(s) 632, 831, 923
BcoDI GTCTC 2 cut(s) 912, 943
BcuI ACTAGT 2 cut(s) 32, 350
BfaI CTAG 5 cut(s) 33, 116, 351, 434, 1276
BfmI CTRYAG 1 cut(s) 16
BfoI RGCGCY 1 cut(s) 844
BisI GCNGC 4 cut(s) 19, 22, 590, 1259
BlsI GCNGC 4 cut(s) 20, 23, 591, 1260
BmcAI AGTACT 2 cut(s) 31, 349
Bme1390I CCNGG 3 cut(s) 632, 831, 923
BmeT110I CYCGRG 1 cut(s) 820
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 2 cut(s) 427, 491
BmiI GGNNCC 5 cut(s) 6, 282, 429, 507, 1233
BmrFI CCNGG 3 cut(s) 632, 831, 923
BmrI ACTGGG 1 cut(s) 339
BmsI GCATC 5 cut(s) 120, 170, 317, 428, 655
BmuI ACTGGG 1 cut(s) 339
BpiI GAAGAC 1 cut(s) 598
BpmI CTGGAG 1 cut(s) 1250
Bsa29I ATCGAT 1 cut(s) 996
BsaI GGTCTC 1 cut(s) 912
BsaJI CCNNGG 3 cut(s) 423, 821, 1097
BsaXI ACNNNNNCTCC 6 cut(s) 238, 268, 923, 953, 1223, 1253
Bsc4I CCNNNNNNNGG 3 cut(s) 642, 836, 922
Bse1I ACTGG 3 cut(s) 272, 339, 345
BseBI CCWGG 3 cut(s) 632, 831, 923
BseCI ATCGAT 1 cut(s) 996
BseDI CCNNGG 3 cut(s) 423, 821, 1097
BseGI GGATG 6 cut(s) 111, 161, 419, 670, 1093, 1115
BseLI CCNNNNNNNGG 3 cut(s) 642, 836, 922
BseMII CTCAG 1 cut(s) 1041
BseNI ACTGG 3 cut(s) 272, 339, 345
BseRI GAGGAG 1 cut(s) 416
BseSI GKGCMC 2 cut(s) 564, 915
BseXI GCAGC 4 cut(s) 30, 33, 601, 1245
Bsh1236I CGCG 1 cut(s) 566
Bsh1285I CGRYCG 1 cut(s) 946
BshFI GGCC 6 cut(s) 428, 493, 635, 862, 926, 1114
BshNI GGYRCC 2 cut(s) 4, 280
BshVI ATCGAT 1 cut(s) 996
BsiEI CGRYCG 1 cut(s) 946
BsiHKAI GWGCWC 3 cut(s) 564, 915, 1093
BsiHKCI CYCGRG 1 cut(s) 820
BslFI GGGAC 3 cut(s) 327, 530, 546
BslI CCNNNNNNNGG 3 cut(s) 642, 836, 922
BsmAI GTCTC 2 cut(s) 912, 943
BsmFI GGGAC 3 cut(s) 327, 530, 546
BsmI GAATGC 2 cut(s) 694, 741
BsnI GGCC 6 cut(s) 428, 493, 635, 862, 926, 1114
Bso31I GGTCTC 1 cut(s) 912
BsoBI CYCGRG 1 cut(s) 820
Bsp1286I GDGCHC 3 cut(s) 564, 915, 1093
Bsp143I GATC 4 cut(s) 893, 943, 1039, 1154
Bsp19I CCATGG 1 cut(s) 423
BspACI CCGC 2 cut(s) 227, 886
BspANI GGCC 6 cut(s) 428, 493, 635, 862, 926, 1114
BspCNI CTCAG 1 cut(s) 1042
BspDI ATCGAT 1 cut(s) 996
BspFNI CGCG 1 cut(s) 566
BspLI GGNNCC 5 cut(s) 6, 282, 429, 507, 1233
BspMAI CTGCAG 1 cut(s) 20
BspPI GGATC 2 cut(s) 901, 1162
BspT107I GGYRCC 2 cut(s) 4, 280
BspTNI GGTCTC 1 cut(s) 912
BsrI ACTGG 3 cut(s) 272, 339, 345
BssECI CCNNGG 3 cut(s) 423, 821, 1097
BssMI GATC 4 cut(s) 893, 943, 1039, 1154
BssSI CACGAG 1 cut(s) 536
BssT1I CCWWGG 1 cut(s) 423
Bst2BI CACGAG 1 cut(s) 536
Bst2UI CCWGG 3 cut(s) 632, 831, 923
Bst4CI ACNGT 3 cut(s) 285, 335, 1163
Bst6I CTCTTC 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 564
BstDEI CTNAG 2 cut(s) 803, 1050
BstDSI CCRYGG 1 cut(s) 423
BstENI CCTNNNNNAGG 1 cut(s) 834
BstF5I GGATG 6 cut(s) 111, 161, 419, 670, 1093, 1115
BstFNI CGCG 1 cut(s) 566
BstH2I RGCGCY 1 cut(s) 844
BstHHI GCGC 2 cut(s) 568, 843
BstKTI GATC 4 cut(s) 896, 946, 1042, 1157
BstMAI GTCTC 2 cut(s) 912, 943
BstMBI GATC 4 cut(s) 893, 943, 1039, 1154
BstMCI CGRYCG 1 cut(s) 946
BstMWI GCNNNNNNNGC 2 cut(s) 425, 804
BstNI CCWGG 3 cut(s) 632, 831, 923
BstNSI RCATGY 1 cut(s) 1030
BstSCI CCNGG 3 cut(s) 630, 829, 921
BstSFI CTRYAG 1 cut(s) 16
BstSLI GKGCMC 2 cut(s) 564, 915
BstUI CGCG 1 cut(s) 566
BstV1I GCAGC 4 cut(s) 30, 33, 601, 1245
BstV2I GAAGAC 1 cut(s) 598
BstX2I RGATCY 1 cut(s) 893
BstYI RGATCY 1 cut(s) 893
Bsu15I ATCGAT 1 cut(s) 996
BsuRI GGCC 6 cut(s) 428, 493, 635, 862, 926, 1114
BsuTUI ATCGAT 1 cut(s) 996
BtgI CCRYGG 1 cut(s) 423
BtgZI GCGATG 1 cut(s) 411
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 6 cut(s) 111, 161, 419, 670, 1093, 1115
BtsI GCAGTG 2 cut(s) 692, 1253
BtsIMutI CAGTG 3 cut(s) 692, 1159, 1253
Cac8I GCNNGC 1 cut(s) 564
CaiI CAGNNNCTG 1 cut(s) 323
CfoI GCGC 2 cut(s) 568, 843
Cfr13I GGNCC 2 cut(s) 427, 491
ClaI ATCGAT 1 cut(s) 996
Csp6I GTAC 5 cut(s) 30, 281, 336, 348, 383
CspCI CAANNNNNGTGG 4 cut(s) 483, 518, 968, 1003
CviAII CATG 5 cut(s) 391, 424, 475, 1027, 1065
CviQI GTAC 5 cut(s) 30, 281, 336, 348, 383
DdeI CTNAG 2 cut(s) 803, 1050
DpnI GATC 4 cut(s) 895, 945, 1041, 1156
DpnII GATC 4 cut(s) 893, 943, 1039, 1154
DrdI GACNNNNNNGTC 2 cut(s) 540, 926
DseDI GACNNNNNNGTC 2 cut(s) 540, 926
EaeI YGGCCR 2 cut(s) 860, 924
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
EciI GGCGGA 2 cut(s) 216, 901
Eco130I CCWWGG 1 cut(s) 423
Eco31I GGTCTC 1 cut(s) 912
Eco88I CYCGRG 1 cut(s) 820
EcoNI CCTNNNNNAGG 1 cut(s) 834
EcoRI GAATTC 2 cut(s) 621, 1143
EcoRII CCWGG 3 cut(s) 630, 829, 921
EcoT14I CCWWGG 1 cut(s) 423
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 5 cut(s) 394, 427, 478, 1030, 1068
FaqI GGGAC 3 cut(s) 327, 530, 546
FatI CATG 5 cut(s) 390, 423, 474, 1026, 1064
Fnu4HI GCNGC 4 cut(s) 19, 22, 590, 1259
FokI GGATG 6 cut(s) 98, 148, 406, 677, 1080, 1122
Fsp4HI GCNGC 4 cut(s) 19, 22, 590, 1259
FspBI CTAG 5 cut(s) 33, 116, 351, 434, 1276
GlaI GCGC 2 cut(s) 567, 842
GluI GCNGC 4 cut(s) 19, 22, 590, 1259
GsuI CTGGAG 1 cut(s) 1250
HaeII RGCGCY 1 cut(s) 844
HaeIII GGCC 6 cut(s) 428, 493, 635, 862, 926, 1114
HhaI GCGC 2 cut(s) 568, 843
Hin1II CATG 5 cut(s) 394, 427, 478, 1030, 1068
Hin6I GCGC 2 cut(s) 566, 841
HinP1I GCGC 2 cut(s) 566, 841
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HindIII AAGCTT 1 cut(s) 796
HinfI GANTC 3 cut(s) 440, 584, 734
HphI GGTGA 2 cut(s) 201, 234
Hpy166II GTNNAC 5 cut(s) 385, 562, 913, 931, 1024
Hpy188I TCNGA 8 cut(s) 462, 714, 733, 943, 948, 1051, 1101, 1154
Hpy188III TCNNGA 4 cut(s) 133, 481, 760, 1229
Hpy8I GTNNAC 5 cut(s) 385, 562, 913, 931, 1024
Hpy99I CGWCG 1 cut(s) 678
HpyAV CCTTC 1 cut(s) 582
HpyCH4III ACNGT 3 cut(s) 285, 335, 1163
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 7 cut(s) 18, 76, 562, 668, 692, 741, 913
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 804
HpyF3I CTNAG 2 cut(s) 803, 1050
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 5 cut(s) 394, 427, 478, 1030, 1068
HspAI GCGC 2 cut(s) 566, 841
KpnI GGTACC 1 cut(s) 284
Kzo9I GATC 4 cut(s) 893, 943, 1039, 1154
LmnI GCTCC 3 cut(s) 511, 1231, 1255
Lsp1109I GCAGC 4 cut(s) 30, 33, 601, 1245
LweI GCATC 5 cut(s) 120, 170, 317, 428, 655
MaeI CTAG 5 cut(s) 33, 116, 351, 434, 1276
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 5 cut(s) 102, 118, 137, 405, 1239
MalI GATC 4 cut(s) 895, 945, 1041, 1156
MboI GATC 4 cut(s) 893, 943, 1039, 1154
MboII GAAGA 4 cut(s) 76, 598, 630, 1176
MflI RGATCY 1 cut(s) 893
MhlI GDGCHC 3 cut(s) 564, 915, 1093
MlsI TGGCCA 1 cut(s) 862
MluCI AATT 7 cut(s) 181, 297, 328, 521, 621, 1103, 1143
MluNI TGGCCA 1 cut(s) 862
MmeI TCCRAC 2 cut(s) 642, 1037
Mox20I TGGCCA 1 cut(s) 862
MscI TGGCCA 1 cut(s) 862
MseI TTAA 6 cut(s) 177, 189, 300, 327, 753, 1137
MslI CAYNNNNRTG 1 cut(s) 557
Msp20I TGGCCA 1 cut(s) 862
MspR9I CCNGG 3 cut(s) 632, 831, 923
Mva1269I GAATGC 2 cut(s) 694, 741
MvaI CCWGG 3 cut(s) 632, 831, 923
MvnI CGCG 1 cut(s) 566
MwoI GCNNNNNNNGC 2 cut(s) 425, 804
NcoI CCATGG 1 cut(s) 423
NdeII GATC 4 cut(s) 893, 943, 1039, 1154
NlaIII CATG 5 cut(s) 394, 427, 478, 1030, 1068
NlaIV GGNNCC 5 cut(s) 6, 282, 429, 507, 1233
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 1 cut(s) 102
NspI RCATGY 1 cut(s) 1030
PciI ACATGT 1 cut(s) 1026
PctI GAATGC 2 cut(s) 694, 741
PfeI GAWTC 3 cut(s) 440, 584, 734
PkrI GCNGC 4 cut(s) 20, 23, 591, 1260
Ple19I CGATCG 1 cut(s) 946
PscI ACATGT 1 cut(s) 1026
PshBI ATTAAT 2 cut(s) 177, 300
PsiI TTATAA 1 cut(s) 782
Psp6I CCWGG 3 cut(s) 630, 829, 921
PspGI CCWGG 3 cut(s) 630, 829, 921
PspN4I GGNNCC 5 cut(s) 6, 282, 429, 507, 1233
PspPI GGNCC 2 cut(s) 427, 491
PstI CTGCAG 1 cut(s) 20
PstNI CAGNNNCTG 1 cut(s) 323
PsuI RGATCY 1 cut(s) 893
PvuI CGATCG 1 cut(s) 946
RsaI GTAC 5 cut(s) 31, 282, 337, 349, 384
RsaNI GTAC 5 cut(s) 30, 281, 336, 348, 383
RseI CAYNNNNRTG 1 cut(s) 557
SaqAI TTAA 6 cut(s) 177, 189, 300, 327, 753, 1137
SatI GCNGC 4 cut(s) 19, 22, 590, 1259
Sau3AI GATC 4 cut(s) 893, 943, 1039, 1154
Sau96I GGNCC 2 cut(s) 427, 491
ScaI AGTACT 2 cut(s) 31, 349
ScrFI CCNGG 3 cut(s) 632, 831, 923
SduI GDGCHC 3 cut(s) 564, 915, 1093
SfaNI GCATC 5 cut(s) 120, 170, 317, 428, 655
SfcI CTRYAG 1 cut(s) 16
SmiMI CAYNNNNRTG 1 cut(s) 557
SpeI ACTAGT 2 cut(s) 32, 350
Sse9I AATT 7 cut(s) 181, 297, 328, 521, 621, 1103, 1143
SsiI CCGC 2 cut(s) 227, 886
SspI AATATT 1 cut(s) 12
SspMI CTAG 5 cut(s) 33, 116, 351, 434, 1276
StyD4I CCNGG 3 cut(s) 630, 829, 921
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 3 cut(s) 285, 335, 1163
TaiI ACGT 1 cut(s) 72
TaqI TCGA 4 cut(s) 616, 656, 835, 996
TaqII GACCGA 1 cut(s) 592
TasI AATT 7 cut(s) 181, 297, 328, 521, 621, 1103, 1143
TatI WGTACW 2 cut(s) 29, 347
TfiI GAWTC 3 cut(s) 440, 584, 734
Tru1I TTAA 6 cut(s) 177, 189, 300, 327, 753, 1137
Tru9I TTAA 6 cut(s) 177, 189, 300, 327, 753, 1137
TscAI CASTG 3 cut(s) 699, 1166, 1260
TseFI GTSAC 1 cut(s) 102
TseI GCWGC 4 cut(s) 18, 21, 589, 1258
Tsp45I GTSAC 1 cut(s) 102
TspDTI ATGAA 4 cut(s) 318, 463, 597, 614
TspRI CASTG 3 cut(s) 699, 1166, 1260
VneI GTGCAC 2 cut(s) 560, 911
VspI ATTAAT 2 cut(s) 177, 300
XagI CCTNNNNNAGG 1 cut(s) 834
XapI RAATTY 4 cut(s) 521, 621, 1103, 1143
XceI RCATGY 1 cut(s) 1030
XspI CTAG 5 cut(s) 33, 116, 351, 434, 1276
ZrmI AGTACT 2 cut(s) 31, 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.