Rw3G027450

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0008998
Physical Location & Seq
Reverse (-)
38992325 .. 38993709
1385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G027450.1

Sequence Viewer

Length: 1242 bp
ATGGCGGTGATATTGTGCAGCAGCAGCAGTGCTAGTGAGCAACATCATCAGGCGTTTCAATTCAATTACGTCCTGCATTTCCTCTTCTTTGTTATTGGTTTGTCACTCGGCATCCTGGCTACCTTATATTTCCAGAGTTACTCATTAGTCACTTTTCAAGCATCTCTCAACTCCATTAATAATTTTATTAACACTCCACTAAAACCATCACCCCCATCACAACCTCTGCCTCCACCGAAATTACCACCACTACAGCTCCACCTCCACCACCACCGCCGCTTCTGGTACTTTAATTACAGTACCAGTCTCAGCACTAGTGTTTCTTTGAAAGAACAAAAGCTGTTAGTACACAACATGAGCGACGAGGAGTTACTTTGGAGAGCATCCATGGCCCCTCAAGTTGATTCGCCTTATAACTGTGTTCCGAAAGTGGCTTTCATGTTCTTGACAAAAGGGCCTCTACCTTTGGCTCCCTTATGGGACAAATTTTTCGAGGGATACGAGGGTCTATACTCTATCTATGTGCATGCGCACCCTTCTTTTAATGAATCGCAGCCTCAAACTTCTGTCTTCCATGGTCGAAGAATTAATAGCCAGGCCGTTTACTGGGGAACTTCCTCAATGTTGGATGCAGAGCGACGGCTATTAGCAAATGCATTGCTTGACTTGTCAAATCAAAGATTTGTATTGCTTTCCGAATCGTGCATTCCCTTGTTCAACTTCACGACAACTTATGACTACCTTGTAAACTCAAATCGAAGCTTTCTAAGCGTATTCGATGACCCGAGGAAGCCTGGTAGAGGGCGCTACAACCCTCAAATGTGGCCCCAAATAAACATCACAGATTGGCGGAAAGGATCTCAATGGTTTGAAGTGCACCGAGATCTGGCCATGCACATTGTCTCTGATCAGAAATACTACCCCATCTTTCAACAATACTGCCACCCTCCTTGTTATATCGATGAGCATTATATTCCAACACTCGTCAACATGTTGTACGCTGATCTCAACTCTGAGAGGAGCATTACATGGGTGGATTGGTCGAGGGGTGGTCCTCATCCTGGGAAATTTGGATGGCCAGATATTACAGATGAGTTTTTGAATGGAATTCGGTTCGGATCGCAGTGCAAATACAACGGCAATACTACTTCATTATGTTTCTTATTTGGTAGGAAGTTTTTGCCTAATGCTCTGGAGCCTTTGTTACGAGTTGCTCCACTGCTGCTTGGCTTTGATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

413

Amino Acids

47.65

Weight (kDa)

7.69

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 144 - 370 5.7e-81 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
Acc16I TGCGCA 1 cut(s) 531
AciI CCGC 4 cut(s) 5, 274, 277, 850
AclWI GGATC 3 cut(s) 865, 1126, 1229
AcoI YGGCCR 2 cut(s) 888, 1076
AcsI RAATTY 3 cut(s) 485, 1067, 1107
AfaI GTAC 4 cut(s) 287, 301, 348, 998
AfiI CCNNNNNNNGG 4 cut(s) 606, 800, 886, 1061
AflIII ACRYGT 1 cut(s) 990
AgsI TTSAA 8 cut(s) 59, 64, 158, 328, 718, 872, 932, 1102
AhlI ACTAGT 1 cut(s) 314
AjnI CCWGG 4 cut(s) 114, 594, 793, 1060
AluBI AGCT 3 cut(s) 256, 340, 762
AluI AGCT 3 cut(s) 256, 340, 762
Alw21I GWGCWC 1 cut(s) 879
Alw26I GTCTC 2 cut(s) 311, 907
Alw44I GTGCAC 1 cut(s) 875
AlwI GGATC 3 cut(s) 865, 1126, 1229
Ama87I CYCGRG 1 cut(s) 784
AoxI GGCC 6 cut(s) 390, 455, 597, 824, 888, 1076
ApaLI GTGCAC 1 cut(s) 875
ApeKI GCWGC 5 cut(s) 18, 21, 24, 553, 1222
ApoI RAATTY 3 cut(s) 485, 1067, 1107
ArsI GACNNNNNNTTYG 2 cut(s) 473, 505
AseI ATTAAT 2 cut(s) 177, 588
AspLEI GCGC 2 cut(s) 532, 807
AspS9I GGNCC 4 cut(s) 391, 455, 825, 1052
AsuHPI GGTGA 2 cut(s) 19, 201
AvaI CYCGRG 1 cut(s) 784
AvaII GGWCC 1 cut(s) 1052
BaeGI GKGCMC 1 cut(s) 879
BaeI ACNNNNGTAYC 2 cut(s) 490, 523
BalI TGGCCA 2 cut(s) 890, 1078
BbsI GAAGAC 1 cut(s) 562
Bbv12I GWGCWC 1 cut(s) 879
BbvI GCAGC 5 cut(s) 30, 33, 36, 565, 1209
BccI CCATC 4 cut(s) 214, 223, 932, 1068
BceAI ACGGC 3 cut(s) 584, 656, 1153
BciT130I CCWGG 4 cut(s) 116, 596, 795, 1062
BciVI GTATCC 1 cut(s) 491
BclI TGATCA 1 cut(s) 907
BcoDI GTCTC 2 cut(s) 311, 907
BcuI ACTAGT 1 cut(s) 314
BfaI CTAG 3 cut(s) 33, 315, 1240
BfmI CTRYAG 1 cut(s) 251
BfoI RGCGCY 1 cut(s) 808
BfuI GTATCC 1 cut(s) 491
BglII AGATCT 1 cut(s) 883
BisI GCNGC 6 cut(s) 19, 22, 25, 277, 554, 1223
BlsI GCNGC 6 cut(s) 20, 23, 26, 278, 555, 1224
Bme1390I CCNGG 4 cut(s) 116, 596, 795, 1062
Bme18I GGWCC 1 cut(s) 1052
BmeT110I CYCGRG 1 cut(s) 784
BmgT120I GGNCC 4 cut(s) 391, 455, 825, 1052
BmiI GGNNCC 4 cut(s) 393, 471, 827, 1197
BmrFI CCNGG 4 cut(s) 116, 596, 795, 1062
BmrI ACTGGG 1 cut(s) 616
BmsI GCATC 4 cut(s) 120, 170, 392, 619
BmuI ACTGGG 1 cut(s) 616
BpiI GAAGAC 1 cut(s) 562
BpmI CTGGAG 1 cut(s) 1214
BpuEI CTTGAG 1 cut(s) 381
Bsa29I ATCGAT 1 cut(s) 960
BsaJI CCNNGG 4 cut(s) 387, 574, 785, 1061
BsaXI ACNNNNNCTCC 4 cut(s) 240, 270, 1187, 1217
Bsc4I CCNNNNNNNGG 4 cut(s) 606, 800, 886, 1061
Bse1I ACTGG 2 cut(s) 303, 611
Bse3DI GCAATG 1 cut(s) 656
BseBI CCWGG 4 cut(s) 116, 596, 795, 1062
BseCI ATCGAT 1 cut(s) 960
BseDI CCNNGG 4 cut(s) 387, 574, 785, 1061
BseGI GGATG 5 cut(s) 111, 383, 634, 1057, 1079
BseLI CCNNNNNNNGG 4 cut(s) 606, 800, 886, 1061
BseMI GCAATG 1 cut(s) 656
BseMII CTCAG 2 cut(s) 322, 1005
BseNI ACTGG 2 cut(s) 303, 611
BseRI GAGGAG 2 cut(s) 380, 1033
BseSI GKGCMC 1 cut(s) 879
BseXI GCAGC 5 cut(s) 30, 33, 36, 565, 1209
BsgI GTGCAG 1 cut(s) 37
BshFI GGCC 6 cut(s) 392, 457, 599, 826, 890, 1078
BshVI ATCGAT 1 cut(s) 960
BsiHKAI GWGCWC 1 cut(s) 879
BsiHKCI CYCGRG 1 cut(s) 784
BslFI GGGAC 1 cut(s) 494
BslI CCNNNNNNNGG 4 cut(s) 606, 800, 886, 1061
BsmAI GTCTC 2 cut(s) 311, 907
BsmFI GGGAC 1 cut(s) 494
BsmI GAATGC 1 cut(s) 705
BsnI GGCC 6 cut(s) 392, 457, 599, 826, 890, 1078
BsoBI CYCGRG 1 cut(s) 784
Bsp1286I GDGCHC 1 cut(s) 879
Bsp143I GATC 6 cut(s) 857, 883, 907, 1003, 1118, 1234
Bsp19I CCATGG 2 cut(s) 387, 574
BspACI CCGC 4 cut(s) 5, 274, 277, 850
BspANI GGCC 6 cut(s) 392, 457, 599, 826, 890, 1078
BspCNI CTCAG 2 cut(s) 321, 1006
BspDI ATCGAT 1 cut(s) 960
BspLI GGNNCC 4 cut(s) 393, 471, 827, 1197
BspPI GGATC 3 cut(s) 865, 1126, 1229
BsrDI GCAATG 1 cut(s) 656
BsrI ACTGG 2 cut(s) 303, 611
BssECI CCNNGG 4 cut(s) 387, 574, 785, 1061
BssMI GATC 6 cut(s) 857, 883, 907, 1003, 1118, 1234
BssT1I CCWWGG 2 cut(s) 387, 574
Bst2UI CCWGG 4 cut(s) 116, 596, 795, 1062
Bst4CI ACNGT 2 cut(s) 299, 419
Bst6I CTCTTC 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 3 cut(s) 308, 767, 1014
BstDSI CCRYGG 2 cut(s) 387, 574
BstENI CCTNNNNNAGG 1 cut(s) 798
BstF5I GGATG 5 cut(s) 111, 383, 634, 1057, 1079
BstH2I RGCGCY 1 cut(s) 808
BstHHI GCGC 2 cut(s) 532, 807
BstKTI GATC 6 cut(s) 860, 886, 910, 1006, 1121, 1237
BstMAI GTCTC 2 cut(s) 311, 907
BstMBI GATC 6 cut(s) 857, 883, 907, 1003, 1118, 1234
BstMWI GCNNNNNNNGC 3 cut(s) 24, 389, 768
BstNI CCWGG 4 cut(s) 116, 596, 795, 1062
BstNSI RCATGY 2 cut(s) 530, 994
BstSCI CCNGG 4 cut(s) 114, 594, 793, 1060
BstSFI CTRYAG 1 cut(s) 251
BstSLI GKGCMC 1 cut(s) 879
BstV1I GCAGC 5 cut(s) 30, 33, 36, 565, 1209
BstV2I GAAGAC 1 cut(s) 562
BstX2I RGATCY 2 cut(s) 857, 883
BstYI RGATCY 2 cut(s) 857, 883
Bsu15I ATCGAT 1 cut(s) 960
BsuI GTATCC 1 cut(s) 491
BsuRI GGCC 6 cut(s) 392, 457, 599, 826, 890, 1078
BsuTUI ATCGAT 1 cut(s) 960
BtgI CCRYGG 2 cut(s) 387, 574
BtsCI GGATG 5 cut(s) 111, 383, 634, 1057, 1079
BtsI GCAGTG 3 cut(s) 34, 1130, 1217
BtsIMutI CAGTG 3 cut(s) 34, 1130, 1217
Cac8I GCNNGC 1 cut(s) 528
CfoI GCGC 2 cut(s) 532, 807
Cfr13I GGNCC 4 cut(s) 391, 455, 825, 1052
ClaI ATCGAT 1 cut(s) 960
Csp6I GTAC 4 cut(s) 286, 300, 347, 997
CspCI CAANNNNNGTGG 2 cut(s) 932, 967
CviAII CATG 8 cut(s) 355, 388, 439, 527, 575, 892, 991, 1029
CviQI GTAC 4 cut(s) 286, 300, 347, 997
DdeI CTNAG 3 cut(s) 308, 767, 1014
DpnI GATC 6 cut(s) 859, 885, 909, 1005, 1120, 1236
DpnII GATC 6 cut(s) 857, 883, 907, 1003, 1118, 1234
EaeI YGGCCR 2 cut(s) 888, 1076
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
EciI GGCGGA 1 cut(s) 865
Eco130I CCWWGG 2 cut(s) 387, 574
Eco47I GGWCC 1 cut(s) 1052
Eco88I CYCGRG 1 cut(s) 784
EcoNI CCTNNNNNAGG 1 cut(s) 798
EcoO109I RGGNCCY 1 cut(s) 455
EcoRI GAATTC 1 cut(s) 1107
EcoRII CCWGG 4 cut(s) 114, 594, 793, 1060
EcoT14I CCWWGG 2 cut(s) 387, 574
EcoT22I ATGCAT 1 cut(s) 658
ErhI CCWWGG 2 cut(s) 387, 574
FaeI CATG 8 cut(s) 358, 391, 442, 530, 578, 895, 994, 1032
FaqI GGGAC 1 cut(s) 494
FatI CATG 8 cut(s) 354, 387, 438, 526, 574, 891, 990, 1028
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 6 cut(s) 19, 22, 25, 277, 554, 1223
FokI GGATG 5 cut(s) 98, 370, 641, 1044, 1086
Fsp4HI GCNGC 6 cut(s) 19, 22, 25, 277, 554, 1223
FspAI RTGCGCAY 1 cut(s) 531
FspBI CTAG 3 cut(s) 33, 315, 1240
FspI TGCGCA 1 cut(s) 531
GlaI GCGC 2 cut(s) 531, 806
GluI GCNGC 6 cut(s) 19, 22, 25, 277, 554, 1223
GsuI CTGGAG 1 cut(s) 1214
HaeII RGCGCY 1 cut(s) 808
HaeIII GGCC 6 cut(s) 392, 457, 599, 826, 890, 1078
HhaI GCGC 2 cut(s) 532, 807
Hin1II CATG 8 cut(s) 358, 391, 442, 530, 578, 895, 994, 1032
Hin6I GCGC 2 cut(s) 530, 805
HinP1I GCGC 2 cut(s) 530, 805
HincII GTYRAC 1 cut(s) 988
HindII GTYRAC 1 cut(s) 988
HindIII AAGCTT 1 cut(s) 760
HinfI GANTC 3 cut(s) 404, 548, 698
HphI GGTGA 2 cut(s) 19, 201
Hpy166II GTNNAC 5 cut(s) 349, 604, 748, 877, 988
Hpy188I TCNGA 6 cut(s) 426, 697, 907, 912, 1015, 1118
Hpy188III TCNNGA 4 cut(s) 133, 445, 724, 1193
Hpy8I GTNNAC 5 cut(s) 349, 604, 748, 877, 988
Hpy99I CGWCG 2 cut(s) 365, 642
HpyAV CCTTC 1 cut(s) 546
HpyCH4III ACNGT 2 cut(s) 299, 419
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 9 cut(s) 18, 76, 526, 632, 656, 705, 877, 895, 1128
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 389, 768
HpyF3I CTNAG 3 cut(s) 308, 767, 1014
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 8 cut(s) 358, 391, 442, 530, 578, 895, 994, 1032
HspAI GCGC 2 cut(s) 530, 805
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 6 cut(s) 857, 883, 907, 1003, 1118, 1234
LmnI GCTCC 5 cut(s) 261, 475, 1020, 1195, 1219
Lsp1109I GCAGC 5 cut(s) 30, 33, 36, 565, 1209
LweI GCATC 4 cut(s) 120, 170, 392, 619
MaeI CTAG 3 cut(s) 33, 315, 1240
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 5 cut(s) 102, 137, 148, 369, 1203
MalI GATC 6 cut(s) 859, 885, 909, 1005, 1120, 1236
MboI GATC 6 cut(s) 857, 883, 907, 1003, 1118, 1234
MboII GAAGA 3 cut(s) 76, 562, 594
MflI RGATCY 2 cut(s) 857, 883
MhlI GDGCHC 1 cut(s) 879
MlsI TGGCCA 2 cut(s) 890, 1078
MluCI AATT 9 cut(s) 59, 64, 181, 239, 292, 485, 585, 1067, 1107
MluNI TGGCCA 2 cut(s) 890, 1078
MmeI TCCRAC 2 cut(s) 606, 1001
Mox20I TGGCCA 2 cut(s) 890, 1078
Mph1103I ATGCAT 1 cut(s) 658
MscI TGGCCA 2 cut(s) 890, 1078
MseI TTAA 5 cut(s) 177, 189, 291, 543, 588
Msp20I TGGCCA 2 cut(s) 890, 1078
MspR9I CCNGG 4 cut(s) 116, 596, 795, 1062
Mva1269I GAATGC 1 cut(s) 705
MvaI CCWGG 4 cut(s) 116, 596, 795, 1062
MwoI GCNNNNNNNGC 3 cut(s) 24, 389, 768
NcoI CCATGG 2 cut(s) 387, 574
NdeII GATC 6 cut(s) 857, 883, 907, 1003, 1118, 1234
NlaIII CATG 8 cut(s) 358, 391, 442, 530, 578, 895, 994, 1032
NlaIV GGNNCC 4 cut(s) 393, 471, 827, 1197
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 2 cut(s) 102, 148
NsbI TGCGCA 1 cut(s) 531
NsiI ATGCAT 1 cut(s) 658
NspI RCATGY 2 cut(s) 530, 994
PaeI GCATGC 1 cut(s) 530
PciI ACATGT 1 cut(s) 990
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PctI GAATGC 1 cut(s) 705
PfeI GAWTC 3 cut(s) 404, 548, 698
PkrI GCNGC 6 cut(s) 20, 23, 26, 278, 555, 1224
PscI ACATGT 1 cut(s) 990
PshBI ATTAAT 2 cut(s) 177, 588
PsiI TTATAA 1 cut(s) 414
Psp6I CCWGG 4 cut(s) 114, 594, 793, 1060
PspGI CCWGG 4 cut(s) 114, 594, 793, 1060
PspN4I GGNNCC 4 cut(s) 393, 471, 827, 1197
PspPI GGNCC 4 cut(s) 391, 455, 825, 1052
PsuI RGATCY 2 cut(s) 857, 883
RsaI GTAC 4 cut(s) 287, 301, 348, 998
RsaNI GTAC 4 cut(s) 286, 300, 347, 997
SaqAI TTAA 5 cut(s) 177, 189, 291, 543, 588
SatI GCNGC 6 cut(s) 19, 22, 25, 277, 554, 1223
Sau3AI GATC 6 cut(s) 857, 883, 907, 1003, 1118, 1234
Sau96I GGNCC 4 cut(s) 391, 455, 825, 1052
ScrFI CCNGG 4 cut(s) 116, 596, 795, 1062
SduI GDGCHC 1 cut(s) 879
SetI ASST 9 cut(s) 72, 125, 226, 258, 264, 342, 466, 744, 764
SfaNI GCATC 4 cut(s) 120, 170, 392, 619
SfcI CTRYAG 1 cut(s) 251
SinI GGWCC 1 cut(s) 1052
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
SpeI ACTAGT 1 cut(s) 314
SphI GCATGC 1 cut(s) 530
Sse9I AATT 9 cut(s) 59, 64, 181, 239, 292, 485, 585, 1067, 1107
SsiI CCGC 4 cut(s) 5, 274, 277, 850
SspMI CTAG 3 cut(s) 33, 315, 1240
StyD4I CCNGG 4 cut(s) 114, 594, 793, 1060
StyI CCWWGG 2 cut(s) 387, 574
TaaI ACNGT 2 cut(s) 299, 419
TaiI ACGT 1 cut(s) 72
TaqI TCGA 6 cut(s) 492, 580, 757, 777, 960, 1043
TasI AATT 9 cut(s) 59, 64, 181, 239, 292, 485, 585, 1067, 1107
TatI WGTACW 1 cut(s) 346
TauI GCSGC 1 cut(s) 279
TfiI GAWTC 3 cut(s) 404, 548, 698
Tru1I TTAA 5 cut(s) 177, 189, 291, 543, 588
Tru9I TTAA 5 cut(s) 177, 189, 291, 543, 588
TscAI CASTG 3 cut(s) 34, 1130, 1224
TseFI GTSAC 2 cut(s) 102, 148
TseI GCWGC 5 cut(s) 18, 21, 24, 553, 1222
Tsp45I GTSAC 2 cut(s) 102, 148
TspDTI ATGAA 3 cut(s) 427, 561, 1140
TspRI CASTG 3 cut(s) 34, 1130, 1224
VneI GTGCAC 1 cut(s) 875
VpaK11BI GGWCC 1 cut(s) 1052
VspI ATTAAT 2 cut(s) 177, 588
XagI CCTNNNNNAGG 1 cut(s) 798
XapI RAATTY 3 cut(s) 485, 1067, 1107
XceI RCATGY 2 cut(s) 530, 994
XspI CTAG 3 cut(s) 33, 315, 1240
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.