Rh3BG357300

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0008998
Physical Location & Seq
Forward (+)
42705196 .. 42708328
3133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG357300.1

Sequence Viewer

Length: 885 bp
ATGAGCGATGAGGAGTTACTTTGGAGAGCATCCATGGCCCCTCAAGTTGATTCGCCGTACAACTCTGTTCCGAAAGTGGCTTTCATGTTCTTGACAAAAGGGCCACTACCTTTGGCTCCCTTATGGGACAAATTTTTCAAGGGACACGAGGGTCTATACTCCATCTATGTGCACGCGCACCCTTCTTTCAATGAATCGCAGCCTCAAACTTCGGTCTTCCATGGTCGAAGAATTCATAGCCAGGCCGTTTATTGGGGAACTTCCTCGATGTTGGATGCAGAGCTACGGCTATTAGCCAATGCACTGCTTGACTTGTCCAATCAAAGATTTGTATTGCTTTCCGAATCTTGCATTCCCTTGTTTAACTTCACGACAACTTATGACTACCTTATAAACTCAAATCAAAGCTTTCTAAGCGTATACGATGACCCGAGGAAGCCTGGTCGAGGGCGCTACAACCGTCAAATGTGGCCACAAATAAACATCACAGATTGGCGGAAAGGATCTCAATGGTTTGAAGTGCACCGAGACCTGGCCGTCCACATTGTCTCCGATCGGAAATACTACCCCATCTTTCAACAATACTGCCACCCTCCTTGTTATATCGATGAGCATTATATTCCAACATTCGTCAACATGTTGTATGCTGATCTCAACTCTGAGAGAAGCATTACATGGGTGGATTGGTCAAGGGGTGGTGCTCATTCCCGAAAATTTGGATGGCCTGATATTACAGATGAGTTTTTAATGGAATTCGGTTCGGATCGCACTGTTCTGATTGGATGCCTCTATGTCAATGGAATTGCACAGAAGGAAGATTTTATCACAGAACCACCCACATATGTATTAGATTCAACTGAGGACGGTTACTATTGCCGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

294

Amino Acids

34.33

Weight (kDa)

5.79

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 26 - 250 2.4e-81 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 392
AasI GACNNNNNNGTC 2 cut(s) 150, 536
AccI GTMKAC 1 cut(s) 420
AccII CGCG 1 cut(s) 176
AciI CCGC 2 cut(s) 496, 877
AclWI GGATC 2 cut(s) 511, 771
AcoI YGGCCR 2 cut(s) 470, 534
AcsI RAATTY 4 cut(s) 131, 231, 713, 752
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 3 cut(s) 252, 446, 532
AflIII ACRYGT 1 cut(s) 636
AgsI TTSAA 5 cut(s) 139, 190, 518, 578, 855
AjnI CCWGG 3 cut(s) 240, 439, 531
AluBI AGCT 2 cut(s) 283, 408
AluI AGCT 2 cut(s) 283, 408
Alw21I GWGCWC 3 cut(s) 174, 525, 703
Alw26I GTCTC 2 cut(s) 522, 553
Alw44I GTGCAC 2 cut(s) 170, 521
AlwI GGATC 2 cut(s) 511, 771
Ama87I CYCGRG 1 cut(s) 430
AoxI GGCC 6 cut(s) 36, 101, 243, 470, 534, 722
ApaLI GTGCAC 2 cut(s) 170, 521
ApeKI GCWGC 1 cut(s) 199
ApoI RAATTY 4 cut(s) 131, 231, 713, 752
AspLEI GCGC 2 cut(s) 178, 453
AspS9I GGNCC 2 cut(s) 37, 101
AvaI CYCGRG 1 cut(s) 430
BaeGI GKGCMC 2 cut(s) 174, 525
BalI TGGCCA 1 cut(s) 472
BauI CACGAG 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 3 cut(s) 174, 525, 703
BbvI GCAGC 1 cut(s) 211
BccI CCATC 3 cut(s) 170, 578, 714
BceAI ACGGC 4 cut(s) 40, 230, 302, 521
BciT130I CCWGG 3 cut(s) 242, 441, 533
BcoDI GTCTC 2 cut(s) 522, 553
BfoI RGCGCY 1 cut(s) 454
BisI GCNGC 2 cut(s) 200, 877
BlsI GCNGC 2 cut(s) 201, 878
Bme1390I CCNGG 3 cut(s) 242, 441, 533
BmeT110I CYCGRG 1 cut(s) 430
BmgT120I GGNCC 2 cut(s) 37, 101
BmiI GGNNCC 2 cut(s) 39, 117
BmrFI CCNGG 3 cut(s) 242, 441, 533
BmsI GCATC 3 cut(s) 38, 265, 773
BpiI GAAGAC 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 27
Bsa29I ATCGAT 1 cut(s) 606
BsaI GGTCTC 1 cut(s) 522
BsaJI CCNNGG 3 cut(s) 33, 220, 431
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bsc4I CCNNNNNNNGG 3 cut(s) 252, 446, 532
BseBI CCWGG 3 cut(s) 242, 441, 533
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 3 cut(s) 33, 220, 431
BseGI GGATG 4 cut(s) 29, 280, 725, 788
BseLI CCNNNNNNNGG 3 cut(s) 252, 446, 532
BseMII CTCAG 2 cut(s) 651, 849
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 2 cut(s) 174, 525
BseXI GCAGC 1 cut(s) 211
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 556
BshFI GGCC 6 cut(s) 38, 103, 245, 472, 536, 724
BshVI ATCGAT 1 cut(s) 606
BsiEI CGRYCG 1 cut(s) 556
BsiHKAI GWGCWC 3 cut(s) 174, 525, 703
BsiHKCI CYCGRG 1 cut(s) 430
BslFI GGGAC 2 cut(s) 140, 156
BslI CCNNNNNNNGG 3 cut(s) 252, 446, 532
BsmAI GTCTC 2 cut(s) 522, 553
BsmFI GGGAC 2 cut(s) 140, 156
BsmI GAATGC 1 cut(s) 351
BsnI GGCC 6 cut(s) 38, 103, 245, 472, 536, 724
Bso31I GGTCTC 1 cut(s) 522
BsoBI CYCGRG 1 cut(s) 430
Bsp1286I GDGCHC 3 cut(s) 174, 525, 703
Bsp143I GATC 4 cut(s) 503, 553, 649, 763
Bsp19I CCATGG 2 cut(s) 33, 220
BspACI CCGC 2 cut(s) 496, 877
BspANI GGCC 6 cut(s) 38, 103, 245, 472, 536, 724
BspCNI CTCAG 2 cut(s) 652, 850
BspDI ATCGAT 1 cut(s) 606
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 2 cut(s) 39, 117
BspPI GGATC 2 cut(s) 511, 771
BspTNI GGTCTC 1 cut(s) 522
BssECI CCNNGG 3 cut(s) 33, 220, 431
BssMI GATC 4 cut(s) 503, 553, 649, 763
BssNAI GTATAC 1 cut(s) 421
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 2 cut(s) 33, 220
Bst1107I GTATAC 1 cut(s) 421
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 3 cut(s) 242, 441, 533
Bst4CI ACNGT 3 cut(s) 461, 772, 866
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 3 cut(s) 413, 660, 858
BstDSI CCRYGG 2 cut(s) 33, 220
BstENI CCTNNNNNAGG 1 cut(s) 444
BstF5I GGATG 4 cut(s) 29, 280, 725, 788
BstFNI CGCG 1 cut(s) 176
BstH2I RGCGCY 1 cut(s) 454
BstHHI GCGC 2 cut(s) 178, 453
BstKTI GATC 4 cut(s) 506, 556, 652, 766
BstMAI GTCTC 2 cut(s) 522, 553
BstMBI GATC 4 cut(s) 503, 553, 649, 763
BstMCI CGRYCG 1 cut(s) 556
BstMWI GCNNNNNNNGC 2 cut(s) 35, 414
BstNI CCWGG 3 cut(s) 242, 441, 533
BstNSI RCATGY 1 cut(s) 640
BstSCI CCNGG 3 cut(s) 240, 439, 531
BstSLI GKGCMC 2 cut(s) 174, 525
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 1 cut(s) 211
BstV2I GAAGAC 1 cut(s) 208
BstX2I RGATCY 1 cut(s) 503
BstYI RGATCY 1 cut(s) 503
BstZ17I GTATAC 1 cut(s) 421
Bsu15I ATCGAT 1 cut(s) 606
BsuRI GGCC 6 cut(s) 38, 103, 245, 472, 536, 724
BsuTUI ATCGAT 1 cut(s) 606
BtgI CCRYGG 2 cut(s) 33, 220
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 4 cut(s) 29, 280, 725, 788
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 2 cut(s) 302, 768
Cac8I GCNNGC 1 cut(s) 174
CfoI GCGC 2 cut(s) 178, 453
Cfr13I GGNCC 2 cut(s) 37, 101
ClaI ATCGAT 1 cut(s) 606
Csp6I GTAC 1 cut(s) 58
CspCI CAANNNNNGTGG 4 cut(s) 93, 128, 578, 613
CviAII CATG 5 cut(s) 34, 85, 221, 637, 675
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 3 cut(s) 413, 660, 858
DpnI GATC 4 cut(s) 505, 555, 651, 765
DpnII GATC 4 cut(s) 503, 553, 649, 763
DrdI GACNNNNNNGTC 2 cut(s) 150, 536
DseDI GACNNNNNNGTC 2 cut(s) 150, 536
EaeI YGGCCR 2 cut(s) 470, 534
EciI GGCGGA 1 cut(s) 511
Eco130I CCWWGG 2 cut(s) 33, 220
Eco31I GGTCTC 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 430
EcoNI CCTNNNNNAGG 1 cut(s) 444
EcoRI GAATTC 2 cut(s) 231, 752
EcoRII CCWGG 3 cut(s) 240, 439, 531
EcoT14I CCWWGG 2 cut(s) 33, 220
ErhI CCWWGG 2 cut(s) 33, 220
FaeI CATG 5 cut(s) 37, 88, 224, 640, 678
FaqI GGGAC 2 cut(s) 140, 156
FatI CATG 5 cut(s) 33, 84, 220, 636, 674
FauNDI CATATG 1 cut(s) 841
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 2 cut(s) 200, 877
FokI GGATG 4 cut(s) 16, 287, 732, 795
Fsp4HI GCNGC 2 cut(s) 200, 877
GlaI GCGC 2 cut(s) 177, 452
GluI GCNGC 2 cut(s) 200, 877
HaeII RGCGCY 1 cut(s) 454
HaeIII GGCC 6 cut(s) 38, 103, 245, 472, 536, 724
HhaI GCGC 2 cut(s) 178, 453
Hin1II CATG 5 cut(s) 37, 88, 224, 640, 678
Hin6I GCGC 2 cut(s) 176, 451
HinP1I GCGC 2 cut(s) 176, 451
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HindIII AAGCTT 1 cut(s) 406
HinfI GANTC 4 cut(s) 50, 194, 344, 851
Hpy166II GTNNAC 5 cut(s) 172, 421, 523, 541, 634
Hpy188I TCNGA 7 cut(s) 72, 343, 553, 558, 661, 763, 777
Hpy188III TCNNGA 3 cut(s) 91, 370, 708
Hpy8I GTNNAC 5 cut(s) 172, 421, 523, 541, 634
HpyAV CCTTC 2 cut(s) 192, 805
HpyCH4III ACNGT 3 cut(s) 461, 772, 866
HpyCH4V TGCA 6 cut(s) 172, 278, 302, 351, 523, 806
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 414
HpyF3I CTNAG 3 cut(s) 413, 660, 858
Hsp92II CATG 5 cut(s) 37, 88, 224, 640, 678
HspAI GCGC 2 cut(s) 176, 451
Kzo9I GATC 4 cut(s) 503, 553, 649, 763
LmnI GCTCC 1 cut(s) 121
LpnPI CCDG 7 cut(s) 227, 254, 426, 453, 518, 545, 738
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 3 cut(s) 38, 265, 773
MaeIII GTNAC 2 cut(s) 15, 866
MalI GATC 4 cut(s) 505, 555, 651, 765
MboI GATC 4 cut(s) 503, 553, 649, 763
MboII GAAGA 3 cut(s) 208, 240, 827
MflI RGATCY 1 cut(s) 503
MhlI GDGCHC 3 cut(s) 174, 525, 703
MlsI TGGCCA 1 cut(s) 472
MluCI AATT 5 cut(s) 131, 231, 713, 752, 801
MluNI TGGCCA 1 cut(s) 472
MmeI TCCRAC 2 cut(s) 252, 647
Mox20I TGGCCA 1 cut(s) 472
MscI TGGCCA 1 cut(s) 472
MseI TTAA 2 cut(s) 363, 746
MslI CAYNNNNRTG 1 cut(s) 167
Msp20I TGGCCA 1 cut(s) 472
MspR9I CCNGG 3 cut(s) 242, 441, 533
Mva1269I GAATGC 1 cut(s) 351
MvaI CCWGG 3 cut(s) 242, 441, 533
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 2 cut(s) 35, 414
NcoI CCATGG 2 cut(s) 33, 220
NdeI CATATG 1 cut(s) 841
NdeII GATC 4 cut(s) 503, 553, 649, 763
NlaIII CATG 5 cut(s) 37, 88, 224, 640, 678
NlaIV GGNNCC 2 cut(s) 39, 117
NspI RCATGY 1 cut(s) 640
PciI ACATGT 1 cut(s) 636
PctI GAATGC 1 cut(s) 351
PfeI GAWTC 4 cut(s) 50, 194, 344, 851
PkrI GCNGC 2 cut(s) 201, 878
Ple19I CGATCG 1 cut(s) 556
PscI ACATGT 1 cut(s) 636
PsiI TTATAA 1 cut(s) 392
Psp6I CCWGG 3 cut(s) 240, 439, 531
PspGI CCWGG 3 cut(s) 240, 439, 531
PspN4I GGNNCC 2 cut(s) 39, 117
PspPI GGNCC 2 cut(s) 37, 101
PsuI RGATCY 1 cut(s) 503
PvuI CGATCG 1 cut(s) 556
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
RseI CAYNNNNRTG 1 cut(s) 167
SaqAI TTAA 2 cut(s) 363, 746
SatI GCNGC 2 cut(s) 200, 877
Sau3AI GATC 4 cut(s) 503, 553, 649, 763
Sau96I GGNCC 2 cut(s) 37, 101
ScrFI CCNGG 3 cut(s) 242, 441, 533
SduI GDGCHC 3 cut(s) 174, 525, 703
SetI ASST 5 cut(s) 112, 285, 390, 410, 534
SfaNI GCATC 3 cut(s) 38, 265, 773
SmiMI CAYNNNNRTG 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 42
SmoI CTYRAG 1 cut(s) 42
Sse9I AATT 5 cut(s) 131, 231, 713, 752, 801
SsiI CCGC 2 cut(s) 496, 877
StyD4I CCNGG 3 cut(s) 240, 439, 531
StyI CCWWGG 2 cut(s) 33, 220
TaaI ACNGT 3 cut(s) 461, 772, 866
TaqI TCGA 4 cut(s) 226, 266, 445, 606
TaqII GACCGA 1 cut(s) 202
TasI AATT 5 cut(s) 131, 231, 713, 752, 801
TauI GCSGC 1 cut(s) 879
TfiI GAWTC 4 cut(s) 50, 194, 344, 851
Tru1I TTAA 2 cut(s) 363, 746
Tru9I TTAA 2 cut(s) 363, 746
TscAI CASTG 2 cut(s) 309, 775
TseI GCWGC 1 cut(s) 199
TspDTI ATGAA 3 cut(s) 73, 207, 224
TspRI CASTG 2 cut(s) 309, 775
VneI GTGCAC 2 cut(s) 170, 521
XagI CCTNNNNNAGG 1 cut(s) 444
XapI RAATTY 4 cut(s) 131, 231, 713, 752
XceI RCATGY 1 cut(s) 640
XmiI GTMKAC 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.