Rh3AG322100

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0008998
Physical Location & Seq
Forward (+)
44954829 .. 44971982
17154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG322100.1

Sequence Viewer

Length: 834 bp
ATGAGCGATGAGGAGTTACTTTGGAGAGCATCCATGGCCCCTCAAGTTGATTCGCCTTGCAACTCTGTTCCGAAAGTGGCTTTCATGTTCTTGACAAAAGGGCCACTACCTTTGGCTCCCTTATGGGACAAATTTTTCAAGGGACACGAGGGTCTATACTCCATCTTTGTGCACGCGCACCCTTCTTTCAATGAATCGCAGCCTCAAACTTCGGTCTTCCATGGTCGAAGAATTCATAGCCAGGCCGTTTATTGGGGAACTTCCTCGATGTTGGATGCAGAGCGACGGCTATTAGCCAATGCACTGCTTGACTTGTCCAATCAAAGATTTGTATTGCTTTCCGAATCTTGCATTCCCTTGTTTAACTTCACGACAACTTATGACTACCTTATAAACTCAAATCAAAGCTTTCAAAGCGTATACGATGACCCGAGGAAGCCTGGTCGAGGGCGCTACAACCCTCAAATGTGGCCACAAATAAACATCACAGATTGGCGGAAAGGATCTCAATGGTTTGAAGTGCACCGAGACCTGGCCGTCCACATTGTCTCCGATCGGAAATACTACCCCATCTTTCAACAATACTGCCACCCTCCTTGTTATATCGATGAGCATTATATTCCAACATTCGTCAACATGTTGTATGGTGATCTCAACTCTGAGAGAAGCATTACATGGGTGGATTGGTCAAGGGGTGGTGCTCATCCTCGGAAATTTGGATGGCCTGATATTACAGATGAGTTTTTTAATGGAATTCGGTTCGGATCGCACTTCATGCCGAATGGAGTTTACAAACACCAACAACTTAATATGGTTTCTATGGCTGTTACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

277

Amino Acids

32.31

Weight (kDa)

6.86

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 26 - 252 1e-82 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 392
AasI GACNNNNNNGTC 2 cut(s) 150, 536
AccI GTMKAC 1 cut(s) 420
AccII CGCG 1 cut(s) 176
AciI CCGC 1 cut(s) 496
AclWI GGATC 2 cut(s) 511, 772
AcoI YGGCCR 2 cut(s) 470, 534
AcsI RAATTY 4 cut(s) 131, 231, 713, 753
AfiI CCNNNNNNNGG 3 cut(s) 252, 446, 532
AflIII ACRYGT 1 cut(s) 636
AgsI TTSAA 5 cut(s) 139, 190, 413, 518, 578
AjnI CCWGG 3 cut(s) 240, 439, 531
AluBI AGCT 1 cut(s) 408
AluI AGCT 1 cut(s) 408
Alw21I GWGCWC 3 cut(s) 174, 525, 703
Alw26I GTCTC 2 cut(s) 522, 553
Alw44I GTGCAC 2 cut(s) 170, 521
AlwI GGATC 2 cut(s) 511, 772
Ama87I CYCGRG 1 cut(s) 430
AoxI GGCC 6 cut(s) 36, 101, 243, 470, 534, 722
ApaLI GTGCAC 2 cut(s) 170, 521
ApeKI GCWGC 1 cut(s) 199
ApoI RAATTY 4 cut(s) 131, 231, 713, 753
AspLEI GCGC 2 cut(s) 178, 453
AspS9I GGNCC 2 cut(s) 37, 101
AsuHPI GGTGA 1 cut(s) 659
AvaI CYCGRG 1 cut(s) 430
BaeGI GKGCMC 2 cut(s) 174, 525
BalI TGGCCA 1 cut(s) 472
BauI CACGAG 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 3 cut(s) 174, 525, 703
BbvI GCAGC 1 cut(s) 211
BccI CCATC 3 cut(s) 170, 578, 714
BceAI ACGGC 3 cut(s) 230, 302, 521
BciT130I CCWGG 3 cut(s) 242, 441, 533
BcoDI GTCTC 2 cut(s) 522, 553
BfoI RGCGCY 1 cut(s) 454
BisI GCNGC 1 cut(s) 200
BlsI GCNGC 1 cut(s) 201
Bme1390I CCNGG 3 cut(s) 242, 441, 533
BmeT110I CYCGRG 1 cut(s) 430
BmgT120I GGNCC 2 cut(s) 37, 101
BmiI GGNNCC 2 cut(s) 39, 117
BmrFI CCNGG 3 cut(s) 242, 441, 533
BmsI GCATC 2 cut(s) 38, 265
BpiI GAAGAC 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 27
Bsa29I ATCGAT 1 cut(s) 606
BsaAI YACGTR 1 cut(s) 831
BsaI GGTCTC 1 cut(s) 522
BsaJI CCNNGG 4 cut(s) 33, 220, 431, 707
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bsc4I CCNNNNNNNGG 3 cut(s) 252, 446, 532
BseBI CCWGG 3 cut(s) 242, 441, 533
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 4 cut(s) 33, 220, 431, 707
BseGI GGATG 4 cut(s) 29, 280, 703, 725
BseLI CCNNNNNNNGG 3 cut(s) 252, 446, 532
BseMII CTCAG 1 cut(s) 651
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 2 cut(s) 174, 525
BseXI GCAGC 1 cut(s) 211
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 556
BshFI GGCC 6 cut(s) 38, 103, 245, 472, 536, 724
BshVI ATCGAT 1 cut(s) 606
BsiEI CGRYCG 1 cut(s) 556
BsiHKAI GWGCWC 3 cut(s) 174, 525, 703
BsiHKCI CYCGRG 1 cut(s) 430
BslFI GGGAC 2 cut(s) 140, 156
BslI CCNNNNNNNGG 3 cut(s) 252, 446, 532
BsmAI GTCTC 2 cut(s) 522, 553
BsmFI GGGAC 2 cut(s) 140, 156
BsmI GAATGC 1 cut(s) 351
BsnI GGCC 6 cut(s) 38, 103, 245, 472, 536, 724
Bso31I GGTCTC 1 cut(s) 522
BsoBI CYCGRG 1 cut(s) 430
Bsp1286I GDGCHC 3 cut(s) 174, 525, 703
Bsp143I GATC 4 cut(s) 503, 553, 649, 764
Bsp19I CCATGG 2 cut(s) 33, 220
BspACI CCGC 1 cut(s) 496
BspANI GGCC 6 cut(s) 38, 103, 245, 472, 536, 724
BspCNI CTCAG 1 cut(s) 652
BspDI ATCGAT 1 cut(s) 606
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 2 cut(s) 39, 117
BspPI GGATC 2 cut(s) 511, 772
BspTNI GGTCTC 1 cut(s) 522
BssECI CCNNGG 4 cut(s) 33, 220, 431, 707
BssMI GATC 4 cut(s) 503, 553, 649, 764
BssNAI GTATAC 1 cut(s) 421
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 2 cut(s) 33, 220
Bst1107I GTATAC 1 cut(s) 421
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 3 cut(s) 242, 441, 533
BstAPI GCANNNNNTGC 1 cut(s) 775
BstBAI YACGTR 1 cut(s) 831
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 1 cut(s) 660
BstDSI CCRYGG 2 cut(s) 33, 220
BstENI CCTNNNNNAGG 1 cut(s) 444
BstF5I GGATG 4 cut(s) 29, 280, 703, 725
BstFNI CGCG 1 cut(s) 176
BstH2I RGCGCY 1 cut(s) 454
BstHHI GCGC 2 cut(s) 178, 453
BstKTI GATC 4 cut(s) 506, 556, 652, 767
BstMAI GTCTC 2 cut(s) 522, 553
BstMBI GATC 4 cut(s) 503, 553, 649, 764
BstMCI CGRYCG 1 cut(s) 556
BstMWI GCNNNNNNNGC 3 cut(s) 35, 414, 775
BstNI CCWGG 3 cut(s) 242, 441, 533
BstNSI RCATGY 1 cut(s) 640
BstSCI CCNGG 3 cut(s) 240, 439, 531
BstSLI GKGCMC 2 cut(s) 174, 525
BstSNI TACGTA 1 cut(s) 831
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 1 cut(s) 211
BstV2I GAAGAC 1 cut(s) 208
BstX2I RGATCY 1 cut(s) 503
BstYI RGATCY 1 cut(s) 503
BstZ17I GTATAC 1 cut(s) 421
Bsu15I ATCGAT 1 cut(s) 606
BsuRI GGCC 6 cut(s) 38, 103, 245, 472, 536, 724
BsuTUI ATCGAT 1 cut(s) 606
BtgI CCRYGG 2 cut(s) 33, 220
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 4 cut(s) 29, 280, 703, 725
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 1 cut(s) 302
Cac8I GCNNGC 1 cut(s) 174
CfoI GCGC 2 cut(s) 178, 453
Cfr13I GGNCC 2 cut(s) 37, 101
ClaI ATCGAT 1 cut(s) 606
CspCI CAANNNNNGTGG 4 cut(s) 93, 128, 578, 613
CviAII CATG 6 cut(s) 34, 85, 221, 637, 675, 775
DdeI CTNAG 1 cut(s) 660
DpnI GATC 4 cut(s) 505, 555, 651, 766
DpnII GATC 4 cut(s) 503, 553, 649, 764
DrdI GACNNNNNNGTC 2 cut(s) 150, 536
DseDI GACNNNNNNGTC 2 cut(s) 150, 536
EaeI YGGCCR 2 cut(s) 470, 534
EciI GGCGGA 1 cut(s) 511
Eco105I TACGTA 1 cut(s) 831
Eco130I CCWWGG 2 cut(s) 33, 220
Eco31I GGTCTC 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 430
EcoNI CCTNNNNNAGG 1 cut(s) 444
EcoRI GAATTC 2 cut(s) 231, 753
EcoRII CCWGG 3 cut(s) 240, 439, 531
EcoT14I CCWWGG 2 cut(s) 33, 220
ErhI CCWWGG 2 cut(s) 33, 220
FaeI CATG 6 cut(s) 37, 88, 224, 640, 678, 778
FaqI GGGAC 2 cut(s) 140, 156
FatI CATG 6 cut(s) 33, 84, 220, 636, 674, 774
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 1 cut(s) 200
FokI GGATG 4 cut(s) 16, 287, 690, 732
Fsp4HI GCNGC 1 cut(s) 200
GlaI GCGC 2 cut(s) 177, 452
GluI GCNGC 1 cut(s) 200
HaeII RGCGCY 1 cut(s) 454
HaeIII GGCC 6 cut(s) 38, 103, 245, 472, 536, 724
HhaI GCGC 2 cut(s) 178, 453
Hin1II CATG 6 cut(s) 37, 88, 224, 640, 678, 778
Hin6I GCGC 2 cut(s) 176, 451
HinP1I GCGC 2 cut(s) 176, 451
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HindIII AAGCTT 1 cut(s) 406
HinfI GANTC 3 cut(s) 50, 194, 344
HphI GGTGA 1 cut(s) 659
Hpy166II GTNNAC 6 cut(s) 172, 421, 523, 541, 634, 790
Hpy188I TCNGA 7 cut(s) 72, 343, 553, 558, 661, 711, 764
Hpy188III TCNNGA 2 cut(s) 91, 370
Hpy8I GTNNAC 6 cut(s) 172, 421, 523, 541, 634, 790
Hpy99I CGWCG 1 cut(s) 288
HpyAV CCTTC 1 cut(s) 192
HpyCH4IV ACGT 1 cut(s) 830
HpyCH4V TGCA 6 cut(s) 60, 172, 278, 302, 351, 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 414, 775
HpyF3I CTNAG 1 cut(s) 660
HpySE526I ACGT 1 cut(s) 830
Hsp92II CATG 6 cut(s) 37, 88, 224, 640, 678, 778
HspAI GCGC 2 cut(s) 176, 451
Kzo9I GATC 4 cut(s) 503, 553, 649, 764
LmnI GCTCC 1 cut(s) 121
LpnPI CCDG 7 cut(s) 227, 254, 426, 453, 518, 545, 738
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 2 cut(s) 38, 265
MaeII ACGT 1 cut(s) 830
MaeIII GTNAC 2 cut(s) 15, 826
MalI GATC 4 cut(s) 505, 555, 651, 766
MboI GATC 4 cut(s) 503, 553, 649, 764
MboII GAAGA 2 cut(s) 208, 240
MflI RGATCY 1 cut(s) 503
MhlI GDGCHC 3 cut(s) 174, 525, 703
MlsI TGGCCA 1 cut(s) 472
MluCI AATT 4 cut(s) 131, 231, 713, 753
MluNI TGGCCA 1 cut(s) 472
MmeI TCCRAC 2 cut(s) 252, 647
Mox20I TGGCCA 1 cut(s) 472
MscI TGGCCA 1 cut(s) 472
MseI TTAA 3 cut(s) 363, 747, 807
MslI CAYNNNNRTG 1 cut(s) 167
Msp20I TGGCCA 1 cut(s) 472
MspR9I CCNGG 3 cut(s) 242, 441, 533
Mva1269I GAATGC 1 cut(s) 351
MvaI CCWGG 3 cut(s) 242, 441, 533
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 3 cut(s) 35, 414, 775
NcoI CCATGG 2 cut(s) 33, 220
NdeII GATC 4 cut(s) 503, 553, 649, 764
NlaIII CATG 6 cut(s) 37, 88, 224, 640, 678, 778
NlaIV GGNNCC 2 cut(s) 39, 117
NspI RCATGY 1 cut(s) 640
PciI ACATGT 1 cut(s) 636
PctI GAATGC 1 cut(s) 351
PfeI GAWTC 3 cut(s) 50, 194, 344
PkrI GCNGC 1 cut(s) 201
Ple19I CGATCG 1 cut(s) 556
Ppu21I YACGTR 1 cut(s) 831
PscI ACATGT 1 cut(s) 636
PsiI TTATAA 1 cut(s) 392
Psp6I CCWGG 3 cut(s) 240, 439, 531
PspGI CCWGG 3 cut(s) 240, 439, 531
PspN4I GGNNCC 2 cut(s) 39, 117
PspPI GGNCC 2 cut(s) 37, 101
PsuI RGATCY 1 cut(s) 503
PvuI CGATCG 1 cut(s) 556
RseI CAYNNNNRTG 1 cut(s) 167
SaqAI TTAA 3 cut(s) 363, 747, 807
SatI GCNGC 1 cut(s) 200
Sau3AI GATC 4 cut(s) 503, 553, 649, 764
Sau96I GGNCC 2 cut(s) 37, 101
ScrFI CCNGG 3 cut(s) 242, 441, 533
SduI GDGCHC 3 cut(s) 174, 525, 703
SetI ASST 5 cut(s) 112, 390, 410, 534, 833
SfaNI GCATC 2 cut(s) 38, 265
SmiMI CAYNNNNRTG 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 42
SmoI CTYRAG 1 cut(s) 42
SnaBI TACGTA 1 cut(s) 831
Sse9I AATT 4 cut(s) 131, 231, 713, 753
SsiI CCGC 1 cut(s) 496
StyD4I CCNGG 3 cut(s) 240, 439, 531
StyI CCWWGG 2 cut(s) 33, 220
TaiI ACGT 1 cut(s) 833
TaqI TCGA 4 cut(s) 226, 266, 445, 606
TaqII GACCGA 1 cut(s) 202
TasI AATT 4 cut(s) 131, 231, 713, 753
TfiI GAWTC 3 cut(s) 50, 194, 344
Tru1I TTAA 3 cut(s) 363, 747, 807
Tru9I TTAA 3 cut(s) 363, 747, 807
TscAI CASTG 1 cut(s) 309
TseI GCWGC 1 cut(s) 199
TspDTI ATGAA 4 cut(s) 73, 207, 224, 763
TspRI CASTG 1 cut(s) 309
VneI GTGCAC 2 cut(s) 170, 521
XagI CCTNNNNNAGG 1 cut(s) 444
XapI RAATTY 4 cut(s) 131, 231, 713, 753
XceI RCATGY 1 cut(s) 640
XmiI GTMKAC 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.