MD09G1254700.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008998
Physical Location & Seq
Forward (+)
32628599 .. 32630695
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1254700.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGACAATGACGAGTCACGAGCAACCCCAAATTCAATCAACTTTCCGGCCCTTTCGAACCCTAATCCAGTTAAACCCTGTCCTTCATTTCCTATTCTTTGGTATAGGTTTGTCACTAGGTATCATAGTTACCTTATATTTCCAGAGCTTCTCACTCACTACTTTTCAAGCCTCCCTAAATTCCATTAACAATATCTTTCCTTTACAATCATCATCACCTCAAGCTCAATTGTTGCCGCCGCCATTACCAAAACTGCTCTCATCGCTGCCTCCTCCACCATCACCAAAACTGCTCCCCTCGCCGCCTCCACCACCACCATTTCTAATACCATCTTTCTCCAAGAATGGGACATCTAACTATACAAGTGTTTCACTGACAGAACAAAACCTCCTCGTACACAATATGAGTGACGAGGAGTTGTTTCGGAGAGCATCAATGGTTCCTAGAATTCAAGATTTGCCTTACAAGCTTGTTCCGAAAGTTGCTTTCATGTTCTTGACAAAGGGACCTCTACCTTTGTCTCCCTTGTGGGAGAAGTTTTTCAAGGGACTTGAAGGACTATACTCCATTTTTGTGCACGCACACCCTGATTTCAATGAATCAGTGCCTAAAGATTCTGTCTTCCACGGTAGAAGAATTCATAGCCAGCCGGTCTATTGGGGAACATCAACAATGTTAGATGCCGAGAGACGCCTCTTAGCAAATGGACTTCTTGACATTTCTAACCAAAGATTTGTGCTGTTTTCTGAATCCTGCATCCCTTTATTCAACTTCACAACAGCATATGACTACCTCATCAACTCAAACCAAAGCATCCTGAACTCATTTGATGACCCTAGAAAGCCAAGCCGGGGCCGATACAACCCTCAAATGTACCCCATGATAAACATCACAAATTGGAGAAAGGGGTCTCAGTGGTTCGAAGTGCACCGTGAGCTTGCCGTCCACATCATTTCTGATAGTAAATATTATCCGATCTTTCAACAGTACTGCAACCCTCCCTGTTACATTGATAAGCACTATATTCCAACACTTGTGAACTTATTGTACACTGAGTTGAGTTCAATGAGGAGCGTCACTTGGGTGGATTGGTCAAGAGGTGGTCCTCATCCTGGAAAATTTGGATGGATTGATATTTCGAATGAGCTTCTGAATCGGATTCGGTTCGGATCAAAATGCACGTATAATGGCAATACAACTTCAGTGTGTTTCTTGTTTGCTAGAAAATTCTTACCTAACACATTGGAGCCTTTGTTGAGGGTTGCCCCTTTGCTACTTGGTTTTGATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

431

Amino Acids

49.18

Weight (kDa)

9.25

Isoelectric Point (pI)

50.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 161 - 388 2.9e-76 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 236, 239, 302
AclWI GGATC 2 cut(s) 1177, 1280
AcsI RAATTY 6 cut(s) 30, 178, 447, 636, 1118, 1226
AcuI CTGAAG 1 cut(s) 1185
AcyI GRCGYC 1 cut(s) 691
AfaI GTAC 4 cut(s) 396, 875, 989, 1049
AfiI CCNNNNNNNGG 3 cut(s) 345, 851, 1112
AgsI TTSAA 9 cut(s) 35, 167, 452, 544, 554, 595, 769, 983, 1065
AjnI CCWGG 1 cut(s) 1111
AleI CACNNNNGTG 1 cut(s) 1082
AloI GAACNNNNNNTCC 2 cut(s) 372, 404
AluBI AGCT 5 cut(s) 147, 224, 469, 937, 1147
AluI AGCT 5 cut(s) 147, 224, 469, 937, 1147
Alw21I GWGCWC 2 cut(s) 579, 930
Alw26I GTCTC 3 cut(s) 525, 682, 915
Alw44I GTGCAC 2 cut(s) 575, 926
AlwI GGATC 2 cut(s) 1177, 1280
AoxI GGCC 2 cut(s) 47, 853
ApaLI GTGCAC 2 cut(s) 575, 926
ApeKI GCWGC 1 cut(s) 265
ApoI RAATTY 6 cut(s) 30, 178, 447, 636, 1118, 1226
Asp700I GAANNNNTTC 1 cut(s) 539
AspS9I GGNCC 4 cut(s) 48, 506, 853, 1103
AsuC2I CCSGG 1 cut(s) 851
AsuHPI GGTGA 2 cut(s) 207, 273
AsuII TTCGAA 3 cut(s) 55, 921, 1139
AvaII GGWCC 2 cut(s) 506, 1103
BaeGI GKGCMC 2 cut(s) 579, 930
BauI CACGAG 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 613
Bbv12I GWGCWC 2 cut(s) 579, 930
BbvI GCAGC 1 cut(s) 252
BccI CCATC 3 cut(s) 286, 337, 1119
BceAI ACGGC 1 cut(s) 926
BciT130I CCWGG 1 cut(s) 1113
BcnI CCSGG 1 cut(s) 851
BcoDI GTCTC 3 cut(s) 525, 682, 915
BfaI CTAG 4 cut(s) 116, 444, 837, 1221
BisI GCNGC 4 cut(s) 236, 239, 266, 302
BlsI GCNGC 4 cut(s) 237, 240, 267, 303
BmcAI AGTACT 1 cut(s) 989
Bme1390I CCNGG 2 cut(s) 851, 1113
Bme18I GGWCC 2 cut(s) 506, 1103
BmgT120I GGNCC 4 cut(s) 48, 506, 853, 1103
BmiI GGNNCC 4 cut(s) 441, 507, 854, 1248
BmrFI CCNGG 2 cut(s) 851, 1113
BmsI GCATC 4 cut(s) 440, 670, 765, 822
BpiI GAAGAC 1 cut(s) 613
Bpu14I TTCGAA 3 cut(s) 55, 921, 1139
BpuEI CTTGAG 1 cut(s) 204
BpuMI CCSGG 1 cut(s) 851
BsaAI YACGTR 1 cut(s) 1182
BsaBI GATNNNNATC 1 cut(s) 887
BsaHI GRCGYC 1 cut(s) 691
BsaI GGTCTC 1 cut(s) 915
BsaJI CCNNGG 2 cut(s) 625, 850
BsaXI ACNNNNNCTCC 4 cut(s) 372, 402, 1238, 1268
Bsc4I CCNNNNNNNGG 3 cut(s) 345, 851, 1112
Bse118I RCCGGY 1 cut(s) 649
Bse1I ACTGG 1 cut(s) 67
Bse8I GATNNNNATC 1 cut(s) 887
BseBI CCWGG 1 cut(s) 1113
BseDI CCNNGG 2 cut(s) 625, 850
BseGI GGATG 4 cut(s) 756, 813, 1108, 1130
BseJI GATNNNNATC 1 cut(s) 887
BseLI CCNNNNNNNGG 3 cut(s) 345, 851, 1112
BseMII CTCAG 2 cut(s) 926, 1044
BseNI ACTGG 1 cut(s) 67
BseRI GAGGAG 4 cut(s) 261, 380, 428, 1084
BseSI GKGCMC 2 cut(s) 579, 930
BseXI GCAGC 1 cut(s) 252
BshFI GGCC 2 cut(s) 49, 855
BsiHKAI GWGCWC 2 cut(s) 579, 930
BsiSI CCGG 3 cut(s) 46, 650, 850
BslFI GGGAC 3 cut(s) 361, 519, 561
BslI CCNNNNNNNGG 3 cut(s) 345, 851, 1112
BsmAI GTCTC 3 cut(s) 525, 682, 915
BsmBI CGTCTC 1 cut(s) 682
BsmFI GGGAC 3 cut(s) 361, 519, 561
BsnI GGCC 2 cut(s) 49, 855
Bso31I GGTCTC 1 cut(s) 915
Bsp119I TTCGAA 3 cut(s) 55, 921, 1139
Bsp1286I GDGCHC 2 cut(s) 579, 930
Bsp1407I TGTACA 1 cut(s) 1047
Bsp143I GATC 3 cut(s) 975, 1169, 1285
BspACI CCGC 3 cut(s) 236, 239, 302
BspANI GGCC 2 cut(s) 49, 855
BspCNI CTCAG 2 cut(s) 925, 1045
BspLI GGNNCC 4 cut(s) 441, 507, 854, 1248
BspPI GGATC 2 cut(s) 1177, 1280
BspT104I TTCGAA 3 cut(s) 55, 921, 1139
BspTNI GGTCTC 1 cut(s) 915
BsrFI RCCGGY 1 cut(s) 649
BsrGI TGTACA 1 cut(s) 1047
BsrI ACTGG 1 cut(s) 67
BssAI RCCGGY 1 cut(s) 649
BssECI CCNNGG 2 cut(s) 625, 850
BssMI GATC 3 cut(s) 975, 1169, 1285
BssNI GRCGYC 1 cut(s) 691
BssSI CACGAG 1 cut(s) 17
Bst2BI CACGAG 1 cut(s) 17
Bst2UI CCWGG 1 cut(s) 1113
Bst4CI ACNGT 3 cut(s) 629, 932, 987
BstACI GRCGYC 1 cut(s) 691
BstAUI TGTACA 1 cut(s) 1047
BstBAI YACGTR 1 cut(s) 1182
BstBI TTCGAA 3 cut(s) 55, 921, 1139
BstC8I GCNNGC 3 cut(s) 579, 647, 939
BstDEI CTNAG 3 cut(s) 697, 912, 1053
BstDSI CCRYGG 1 cut(s) 625
BstF5I GGATG 4 cut(s) 756, 813, 1108, 1130
BstKTI GATC 3 cut(s) 978, 1172, 1288
BstMAI GTCTC 3 cut(s) 525, 682, 915
BstMBI GATC 3 cut(s) 975, 1169, 1285
BstMWI GCNNNNNNNGC 4 cut(s) 262, 298, 466, 934
BstNI CCWGG 1 cut(s) 1113
BstSCI CCNGG 2 cut(s) 849, 1111
BstSLI GKGCMC 2 cut(s) 579, 930
BstV1I GCAGC 1 cut(s) 252
BstV2I GAAGAC 1 cut(s) 613
BsuRI GGCC 2 cut(s) 49, 855
BtgI CCRYGG 1 cut(s) 625
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 4 cut(s) 756, 813, 1108, 1130
BtsIMutI CAGTG 5 cut(s) 371, 609, 920, 1050, 1209
Cac8I GCNNGC 3 cut(s) 579, 647, 939
Cfr10I RCCGGY 1 cut(s) 649
Cfr13I GGNCC 4 cut(s) 48, 506, 853, 1103
CseI GACGC 2 cut(s) 699, 1063
Csp6I GTAC 4 cut(s) 395, 874, 988, 1048
CviAII CATG 2 cut(s) 490, 880
CviQI GTAC 4 cut(s) 395, 874, 988, 1048
DdeI CTNAG 3 cut(s) 697, 912, 1053
DpnI GATC 3 cut(s) 977, 1171, 1287
DpnII GATC 3 cut(s) 975, 1169, 1285
Eco31I GGTCTC 1 cut(s) 915
Eco47I GGWCC 2 cut(s) 506, 1103
Eco57I CTGAAG 1 cut(s) 1185
EcoO109I RGGNCCY 1 cut(s) 506
EcoRI GAATTC 2 cut(s) 447, 636
EcoRII CCWGG 1 cut(s) 1111
Esp3I CGTCTC 1 cut(s) 682
FaeI CATG 2 cut(s) 493, 883
FaqI GGGAC 3 cut(s) 361, 519, 561
FatI CATG 2 cut(s) 489, 879
FauNDI CATATG 1 cut(s) 784
Fnu4HI GCNGC 4 cut(s) 236, 239, 266, 302
FokI GGATG 4 cut(s) 743, 800, 1095, 1137
Fsp4HI GCNGC 4 cut(s) 236, 239, 266, 302
FspBI CTAG 4 cut(s) 116, 444, 837, 1221
GluI GCNGC 4 cut(s) 236, 239, 266, 302
HaeIII GGCC 2 cut(s) 49, 855
HapII CCGG 3 cut(s) 46, 650, 850
HgaI GACGC 2 cut(s) 699, 1063
Hin1I GRCGYC 1 cut(s) 691
Hin1II CATG 2 cut(s) 493, 883
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 6 cut(s) 13, 599, 614, 749, 1153, 1159
HpaII CCGG 3 cut(s) 46, 650, 850
HphI GGTGA 2 cut(s) 207, 273
Hpy166II GTNNAC 6 cut(s) 397, 577, 928, 946, 1039, 1050
Hpy188I TCNGA 8 cut(s) 426, 477, 748, 958, 975, 1152, 1158, 1169
Hpy188III TCNNGA 7 cut(s) 17, 142, 452, 496, 713, 817, 1095
Hpy8I GTNNAC 6 cut(s) 397, 577, 928, 946, 1039, 1050
HpyAV CCTTC 2 cut(s) 92, 548
HpyCH4III ACNGT 3 cut(s) 629, 932, 987
HpyCH4IV ACGT 1 cut(s) 1181
HpyCH4V TGCA 5 cut(s) 577, 756, 928, 993, 1179
HpyF10VI GCNNNNNNNGC 4 cut(s) 262, 298, 466, 934
HpyF3I CTNAG 3 cut(s) 697, 912, 1053
HpySE526I ACGT 1 cut(s) 1181
Hsp92I GRCGYC 1 cut(s) 691
Hsp92II CATG 2 cut(s) 493, 883
Kzo9I GATC 3 cut(s) 975, 1169, 1285
LmnI GCTCC 3 cut(s) 297, 1071, 1246
Lsp1109I GCAGC 1 cut(s) 252
LweI GCATC 4 cut(s) 440, 670, 765, 822
MaeI CTAG 4 cut(s) 116, 444, 837, 1221
MaeII ACGT 1 cut(s) 1181
MaeIII GTNAC 6 cut(s) 14, 111, 127, 407, 1004, 1075
MalI GATC 3 cut(s) 977, 1171, 1287
MboI GATC 3 cut(s) 975, 1169, 1285
MboII GAAGA 2 cut(s) 613, 645
MfeI CAATTG 1 cut(s) 227
MhlI GDGCHC 2 cut(s) 579, 930
MluCI AATT 8 cut(s) 30, 178, 227, 447, 636, 895, 1118, 1226
MlyI GAGTC 1 cut(s) 22
MmeI TCCRAC 1 cut(s) 1052
MroXI GAANNNNTTC 1 cut(s) 539
MseI TTAA 2 cut(s) 71, 186
MslI CAYNNNNRTG 2 cut(s) 572, 1082
MspI CCGG 3 cut(s) 46, 650, 850
MspR9I CCNGG 2 cut(s) 851, 1113
MunI CAATTG 1 cut(s) 227
MvaI CCWGG 1 cut(s) 1113
MwoI GCNNNNNNNGC 4 cut(s) 262, 298, 466, 934
NciI CCSGG 1 cut(s) 851
NdeI CATATG 1 cut(s) 784
NdeII GATC 3 cut(s) 975, 1169, 1285
NlaIII CATG 2 cut(s) 493, 883
NlaIV GGNNCC 4 cut(s) 441, 507, 854, 1248
NmeAIII GCCGAG 1 cut(s) 709
NmuCI GTSAC 4 cut(s) 14, 111, 407, 1075
NspV TTCGAA 3 cut(s) 55, 921, 1139
OliI CACNNNNGTG 1 cut(s) 1082
PdmI GAANNNNTTC 1 cut(s) 539
PfeI GAWTC 5 cut(s) 599, 614, 749, 1153, 1159
PfoI TCCNGGA 1 cut(s) 1111
PkrI GCNGC 4 cut(s) 237, 240, 267, 303
PleI GAGTC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 21
Ppu21I YACGTR 1 cut(s) 1182
PpuMI RGGWCCY 1 cut(s) 506
Psp5II RGGWCCY 1 cut(s) 506
Psp6I CCWGG 1 cut(s) 1111
PspGI CCWGG 1 cut(s) 1111
PspN4I GGNNCC 4 cut(s) 441, 507, 854, 1248
PspPI GGNCC 4 cut(s) 48, 506, 853, 1103
PspPPI RGGWCCY 1 cut(s) 506
PsrI GAACNNNNNNTAC 2 cut(s) 1031, 1063
RsaI GTAC 4 cut(s) 396, 875, 989, 1049
RsaNI GTAC 4 cut(s) 395, 874, 988, 1048
RseI CAYNNNNRTG 2 cut(s) 572, 1082
SaqAI TTAA 2 cut(s) 71, 186
SatI GCNGC 4 cut(s) 236, 239, 266, 302
Sau3AI GATC 3 cut(s) 975, 1169, 1285
Sau96I GGNCC 4 cut(s) 48, 506, 853, 1103
ScaI AGTACT 1 cut(s) 989
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 2 cut(s) 851, 1113
SduI GDGCHC 2 cut(s) 579, 930
SfaNI GCATC 4 cut(s) 440, 670, 765, 822
SfuI TTCGAA 3 cut(s) 55, 921, 1139
SinI GGWCC 2 cut(s) 506, 1103
SmiMI CAYNNNNRTG 2 cut(s) 572, 1082
SmlI CTYRAG 1 cut(s) 219
SmoI CTYRAG 1 cut(s) 219
Sse9I AATT 8 cut(s) 30, 178, 227, 447, 636, 895, 1118, 1226
SsiI CCGC 3 cut(s) 236, 239, 302
SspI AATATT 1 cut(s) 968
SspMI CTAG 4 cut(s) 116, 444, 837, 1221
StyD4I CCNGG 2 cut(s) 849, 1111
TaaI ACNGT 3 cut(s) 629, 932, 987
TaiI ACGT 1 cut(s) 1184
TaqI TCGA 3 cut(s) 55, 921, 1139
TasI AATT 8 cut(s) 30, 178, 227, 447, 636, 895, 1118, 1226
TatI WGTACW 2 cut(s) 987, 1047
TauI GCSGC 3 cut(s) 238, 241, 304
TfiI GAWTC 5 cut(s) 599, 614, 749, 1153, 1159
Tru1I TTAA 2 cut(s) 71, 186
Tru9I TTAA 2 cut(s) 71, 186
TscAI CASTG 5 cut(s) 378, 609, 920, 1057, 1209
TseFI GTSAC 4 cut(s) 14, 111, 407, 1075
TseI GCWGC 1 cut(s) 265
Tsp45I GTSAC 4 cut(s) 14, 111, 407, 1075
TspDTI ATGAA 4 cut(s) 74, 478, 612, 629
TspRI CASTG 5 cut(s) 378, 609, 920, 1057, 1209
VneI GTGCAC 2 cut(s) 575, 926
VpaK11BI GGWCC 2 cut(s) 506, 1103
XapI RAATTY 6 cut(s) 30, 178, 447, 636, 1118, 1226
XmnI GAANNNNTTC 1 cut(s) 539
XspI CTAG 4 cut(s) 116, 444, 837, 1221
ZrmI AGTACT 1 cut(s) 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.