Rh3CG346500

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0008998
Physical Location & Seq
Reverse (-)
39461610 .. 39463054
1445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG346500.1

Sequence Viewer

Length: 1284 bp
ATGATGGCGGTGATATTGTGCAGCAGCAGCAGTGCTAGTGAGCAACATCATCAGGCGTTTCAATTCAATTACGTCCTGCATTTCCTCTTCTTTGTTATTGGTTTGTCACTCGGCATCCTGGCTACCTTATATTTCCAGAGTTACTCATTAGTCACTTTTCAAGCATCTCTCAACTCCATTAATAATTTTATTAACACTCCACTAAAACCATCACCCCCATCACAACCTCTGCCTCCACCGAAATTACCACCTACAGCTCCACCTCCACCGCCACCGCCGCTTCTGGTACTGTCTTTCTCAACAATCAATGCAACATCATCATCAGTATTAGTTAATTACAGAACCAGTCTCAGCACTAGTGTTTCTTTGAAAGAACAAAAGCTGTTAGTACACAACATGAGCGACGAGGAGTTACTTTGGAGAGCATCCATGGCCCCTCAAGTTGATTCGCCTTATAACTGTGTTCCGAAAGTGGCTTTCATGTTCTTGACAAAAGGGCCTCTACCTTTGGCTCCCTTATGGGACAAATTTTTCGAGGGATACGAGGGTCTATACTCTATCTATGTGCATGCGCACCCTTCTTTTAATGAATCGCAGCCTCAAACTTCTGTCTTCCATGGTCGAAGAATTAATAGCCAGGCCGTTTACTGGGGAACTTCCTCAATGTTGGATGCAGAGCGACGGCTATTAGCAAATGCATTGCTTGACTTGTCAAATCAAAGATTTGTATTGCTTTCCGAATCGTGCATTCCCTTGTTTAACTTCACGACAACTTATGACTACCTTGTAAACTCAAATCGAAGCTTTCTAAGCGTATTCGATGACCCGAGGAAGCCTGGTAGAGGGCGCTACAACCCTCAAATGTGGCCCCAAATAAACATCACAGATTGGCGGAAAGGATCTCAATGGTTTGAAGTGCACCGAGATCTGGCCATGCACATTGTCTCTGATCAGAAATACTACCCCATCTTTCAACAATACTGCCACCCTCCTTGTTATATCGATGAGCATTATATTCCAACACTTGTCAACATGTTGTACGCTGATCTCAACTCTGAGAGGAGCATTACATGGGTGGATTGGTCGAGGGGTGGTCCTCATCCTGGGAAATTTGGATGGCCAGATATTACAGATGAGTTTTTGAATGGAATTCGGTTCGGATCGCAGTGCAAATACAACGGCAATACTACTTCATTATGTTTCTTATTTGGTAGGAAGTTTTTGCCTAATGCTCTGGAGCCTTTGTTACGAGTTGCTCCACTGCTGCTTGGCTTTGATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

427

Amino Acids

48.57

Weight (kDa)

7.18

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 158 - 384 6.2e-81 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 456
Acc16I TGCGCA 1 cut(s) 573
AciI CCGC 5 cut(s) 8, 269, 275, 278, 892
AclWI GGATC 3 cut(s) 907, 1168, 1271
AcoI YGGCCR 2 cut(s) 930, 1118
AcsI RAATTY 3 cut(s) 527, 1109, 1149
AfaI GTAC 3 cut(s) 288, 390, 1040
AfiI CCNNNNNNNGG 4 cut(s) 648, 842, 928, 1103
AflIII ACRYGT 1 cut(s) 1032
AgsI TTSAA 7 cut(s) 62, 67, 161, 370, 914, 974, 1144
AhlI ACTAGT 1 cut(s) 356
AjnI CCWGG 4 cut(s) 117, 636, 835, 1102
AluBI AGCT 3 cut(s) 257, 382, 804
AluI AGCT 3 cut(s) 257, 382, 804
Alw21I GWGCWC 1 cut(s) 921
Alw26I GTCTC 2 cut(s) 353, 949
Alw44I GTGCAC 1 cut(s) 917
AlwI GGATC 3 cut(s) 907, 1168, 1271
Ama87I CYCGRG 1 cut(s) 826
AoxI GGCC 6 cut(s) 432, 497, 639, 866, 930, 1118
ApaLI GTGCAC 1 cut(s) 917
ApeKI GCWGC 5 cut(s) 21, 24, 27, 595, 1264
ApoI RAATTY 3 cut(s) 527, 1109, 1149
ArsI GACNNNNNNTTYG 2 cut(s) 515, 547
AseI ATTAAT 2 cut(s) 180, 630
AspLEI GCGC 2 cut(s) 574, 849
AspS9I GGNCC 4 cut(s) 433, 497, 867, 1094
AsuHPI GGTGA 2 cut(s) 22, 204
AvaI CYCGRG 1 cut(s) 826
AvaII GGWCC 1 cut(s) 1094
BaeGI GKGCMC 1 cut(s) 921
BaeI ACNNNNGTAYC 2 cut(s) 532, 565
BalI TGGCCA 2 cut(s) 932, 1120
BbsI GAAGAC 1 cut(s) 604
Bbv12I GWGCWC 1 cut(s) 921
BbvI GCAGC 5 cut(s) 33, 36, 39, 607, 1251
BccI CCATC 4 cut(s) 217, 226, 974, 1110
BceAI ACGGC 3 cut(s) 626, 698, 1195
BciT130I CCWGG 4 cut(s) 119, 638, 837, 1104
BciVI GTATCC 1 cut(s) 533
BclI TGATCA 1 cut(s) 949
BcoDI GTCTC 2 cut(s) 353, 949
BcuI ACTAGT 1 cut(s) 356
BfaI CTAG 3 cut(s) 36, 357, 1282
BfmI CTRYAG 1 cut(s) 252
BfoI RGCGCY 1 cut(s) 850
BfuI GTATCC 1 cut(s) 533
BglII AGATCT 1 cut(s) 925
BisI GCNGC 6 cut(s) 22, 25, 28, 278, 596, 1265
BlsI GCNGC 6 cut(s) 23, 26, 29, 279, 597, 1266
Bme1390I CCNGG 4 cut(s) 119, 638, 837, 1104
Bme18I GGWCC 1 cut(s) 1094
BmeT110I CYCGRG 1 cut(s) 826
BmgT120I GGNCC 4 cut(s) 433, 497, 867, 1094
BmiI GGNNCC 4 cut(s) 435, 513, 869, 1239
BmrFI CCNGG 4 cut(s) 119, 638, 837, 1104
BmrI ACTGGG 1 cut(s) 658
BmsI GCATC 4 cut(s) 123, 173, 434, 661
BmuI ACTGGG 1 cut(s) 658
BpiI GAAGAC 1 cut(s) 604
BpmI CTGGAG 1 cut(s) 1256
BpuEI CTTGAG 1 cut(s) 423
Bsa29I ATCGAT 1 cut(s) 1002
BsaJI CCNNGG 4 cut(s) 429, 616, 827, 1103
BsaXI ACNNNNNCTCC 2 cut(s) 1229, 1259
Bsc4I CCNNNNNNNGG 4 cut(s) 648, 842, 928, 1103
Bse1I ACTGG 2 cut(s) 345, 653
Bse3DI GCAATG 1 cut(s) 698
BseBI CCWGG 4 cut(s) 119, 638, 837, 1104
BseCI ATCGAT 1 cut(s) 1002
BseDI CCNNGG 4 cut(s) 429, 616, 827, 1103
BseGI GGATG 5 cut(s) 114, 425, 676, 1099, 1121
BseLI CCNNNNNNNGG 4 cut(s) 648, 842, 928, 1103
BseMI GCAATG 1 cut(s) 698
BseMII CTCAG 2 cut(s) 364, 1047
BseNI ACTGG 2 cut(s) 345, 653
BseRI GAGGAG 2 cut(s) 422, 1075
BseSI GKGCMC 1 cut(s) 921
BseXI GCAGC 5 cut(s) 33, 36, 39, 607, 1251
BsgI GTGCAG 1 cut(s) 40
BshFI GGCC 6 cut(s) 434, 499, 641, 868, 932, 1120
BshVI ATCGAT 1 cut(s) 1002
BsiHKAI GWGCWC 1 cut(s) 921
BsiHKCI CYCGRG 1 cut(s) 826
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 4 cut(s) 648, 842, 928, 1103
BsmAI GTCTC 2 cut(s) 353, 949
BsmFI GGGAC 1 cut(s) 536
BsmI GAATGC 1 cut(s) 747
BsnI GGCC 6 cut(s) 434, 499, 641, 868, 932, 1120
BsoBI CYCGRG 1 cut(s) 826
Bsp1286I GDGCHC 1 cut(s) 921
Bsp143I GATC 6 cut(s) 899, 925, 949, 1045, 1160, 1276
Bsp19I CCATGG 2 cut(s) 429, 616
BspACI CCGC 5 cut(s) 8, 269, 275, 278, 892
BspANI GGCC 6 cut(s) 434, 499, 641, 868, 932, 1120
BspCNI CTCAG 2 cut(s) 363, 1048
BspDI ATCGAT 1 cut(s) 1002
BspLI GGNNCC 4 cut(s) 435, 513, 869, 1239
BspPI GGATC 3 cut(s) 907, 1168, 1271
BsrDI GCAATG 1 cut(s) 698
BsrI ACTGG 2 cut(s) 345, 653
BssECI CCNNGG 4 cut(s) 429, 616, 827, 1103
BssMI GATC 6 cut(s) 899, 925, 949, 1045, 1160, 1276
BssT1I CCWWGG 2 cut(s) 429, 616
Bst2UI CCWGG 4 cut(s) 119, 638, 837, 1104
Bst4CI ACNGT 2 cut(s) 291, 461
Bst6I CTCTTC 1 cut(s) 92
BstC8I GCNNGC 1 cut(s) 570
BstDEI CTNAG 3 cut(s) 350, 809, 1056
BstDSI CCRYGG 2 cut(s) 429, 616
BstENI CCTNNNNNAGG 1 cut(s) 840
BstF5I GGATG 5 cut(s) 114, 425, 676, 1099, 1121
BstH2I RGCGCY 1 cut(s) 850
BstHHI GCGC 2 cut(s) 574, 849
BstKTI GATC 6 cut(s) 902, 928, 952, 1048, 1163, 1279
BstMAI GTCTC 2 cut(s) 353, 949
BstMBI GATC 6 cut(s) 899, 925, 949, 1045, 1160, 1276
BstMWI GCNNNNNNNGC 4 cut(s) 27, 277, 431, 810
BstNI CCWGG 4 cut(s) 119, 638, 837, 1104
BstNSI RCATGY 2 cut(s) 572, 1036
BstSCI CCNGG 4 cut(s) 117, 636, 835, 1102
BstSFI CTRYAG 1 cut(s) 252
BstSLI GKGCMC 1 cut(s) 921
BstV1I GCAGC 5 cut(s) 33, 36, 39, 607, 1251
BstV2I GAAGAC 1 cut(s) 604
BstX2I RGATCY 2 cut(s) 899, 925
BstYI RGATCY 2 cut(s) 899, 925
Bsu15I ATCGAT 1 cut(s) 1002
BsuI GTATCC 1 cut(s) 533
BsuRI GGCC 6 cut(s) 434, 499, 641, 868, 932, 1120
BsuTUI ATCGAT 1 cut(s) 1002
BtgI CCRYGG 2 cut(s) 429, 616
BtsCI GGATG 5 cut(s) 114, 425, 676, 1099, 1121
BtsI GCAGTG 3 cut(s) 37, 1172, 1259
BtsIMutI CAGTG 3 cut(s) 37, 1172, 1259
Cac8I GCNNGC 1 cut(s) 570
CfoI GCGC 2 cut(s) 574, 849
Cfr13I GGNCC 4 cut(s) 433, 497, 867, 1094
ClaI ATCGAT 1 cut(s) 1002
Csp6I GTAC 3 cut(s) 287, 389, 1039
CspCI CAANNNNNGTGG 2 cut(s) 974, 1009
CviAII CATG 8 cut(s) 397, 430, 481, 569, 617, 934, 1033, 1071
CviQI GTAC 3 cut(s) 287, 389, 1039
DdeI CTNAG 3 cut(s) 350, 809, 1056
DpnI GATC 6 cut(s) 901, 927, 951, 1047, 1162, 1278
DpnII GATC 6 cut(s) 899, 925, 949, 1045, 1160, 1276
EaeI YGGCCR 2 cut(s) 930, 1118
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
EciI GGCGGA 1 cut(s) 907
Eco130I CCWWGG 2 cut(s) 429, 616
Eco47I GGWCC 1 cut(s) 1094
Eco88I CYCGRG 1 cut(s) 826
EcoNI CCTNNNNNAGG 1 cut(s) 840
EcoO109I RGGNCCY 1 cut(s) 497
EcoRI GAATTC 1 cut(s) 1149
EcoRII CCWGG 4 cut(s) 117, 636, 835, 1102
EcoT14I CCWWGG 2 cut(s) 429, 616
EcoT22I ATGCAT 1 cut(s) 700
ErhI CCWWGG 2 cut(s) 429, 616
FaeI CATG 8 cut(s) 400, 433, 484, 572, 620, 937, 1036, 1074
FaqI GGGAC 1 cut(s) 536
FatI CATG 8 cut(s) 396, 429, 480, 568, 616, 933, 1032, 1070
FbaI TGATCA 1 cut(s) 949
Fnu4HI GCNGC 6 cut(s) 22, 25, 28, 278, 596, 1265
FokI GGATG 5 cut(s) 101, 412, 683, 1086, 1128
Fsp4HI GCNGC 6 cut(s) 22, 25, 28, 278, 596, 1265
FspAI RTGCGCAY 1 cut(s) 573
FspBI CTAG 3 cut(s) 36, 357, 1282
FspI TGCGCA 1 cut(s) 573
GlaI GCGC 2 cut(s) 573, 848
GluI GCNGC 6 cut(s) 22, 25, 28, 278, 596, 1265
GsuI CTGGAG 1 cut(s) 1256
HaeII RGCGCY 1 cut(s) 850
HaeIII GGCC 6 cut(s) 434, 499, 641, 868, 932, 1120
HhaI GCGC 2 cut(s) 574, 849
Hin1II CATG 8 cut(s) 400, 433, 484, 572, 620, 937, 1036, 1074
Hin6I GCGC 2 cut(s) 572, 847
HinP1I GCGC 2 cut(s) 572, 847
HincII GTYRAC 1 cut(s) 1030
HindII GTYRAC 1 cut(s) 1030
HindIII AAGCTT 1 cut(s) 802
HinfI GANTC 3 cut(s) 446, 590, 740
HphI GGTGA 2 cut(s) 22, 204
Hpy166II GTNNAC 5 cut(s) 391, 646, 790, 919, 1030
Hpy188I TCNGA 6 cut(s) 468, 739, 949, 954, 1057, 1160
Hpy188III TCNNGA 4 cut(s) 136, 487, 766, 1235
Hpy8I GTNNAC 5 cut(s) 391, 646, 790, 919, 1030
Hpy99I CGWCG 2 cut(s) 407, 684
HpyAV CCTTC 1 cut(s) 588
HpyCH4III ACNGT 2 cut(s) 291, 461
HpyCH4IV ACGT 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 277, 431, 810
HpyF3I CTNAG 3 cut(s) 350, 809, 1056
HpySE526I ACGT 1 cut(s) 72
Hsp92II CATG 8 cut(s) 400, 433, 484, 572, 620, 937, 1036, 1074
HspAI GCGC 2 cut(s) 572, 847
Ksp22I TGATCA 1 cut(s) 949
Kzo9I GATC 6 cut(s) 899, 925, 949, 1045, 1160, 1276
LmnI GCTCC 5 cut(s) 262, 517, 1062, 1237, 1261
Lsp1109I GCAGC 5 cut(s) 33, 36, 39, 607, 1251
LweI GCATC 4 cut(s) 123, 173, 434, 661
MaeI CTAG 3 cut(s) 36, 357, 1282
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 5 cut(s) 105, 140, 151, 411, 1245
MalI GATC 6 cut(s) 901, 927, 951, 1047, 1162, 1278
MboI GATC 6 cut(s) 899, 925, 949, 1045, 1160, 1276
MboII GAAGA 3 cut(s) 79, 604, 636
MflI RGATCY 2 cut(s) 899, 925
MhlI GDGCHC 1 cut(s) 921
MlsI TGGCCA 2 cut(s) 932, 1120
MluCI AATT 9 cut(s) 62, 67, 184, 242, 334, 527, 627, 1109, 1149
MluNI TGGCCA 2 cut(s) 932, 1120
MmeI TCCRAC 2 cut(s) 648, 1043
Mox20I TGGCCA 2 cut(s) 932, 1120
Mph1103I ATGCAT 1 cut(s) 700
MscI TGGCCA 2 cut(s) 932, 1120
MseI TTAA 6 cut(s) 180, 192, 333, 585, 630, 759
Msp20I TGGCCA 2 cut(s) 932, 1120
MspR9I CCNGG 4 cut(s) 119, 638, 837, 1104
Mva1269I GAATGC 1 cut(s) 747
MvaI CCWGG 4 cut(s) 119, 638, 837, 1104
MwoI GCNNNNNNNGC 4 cut(s) 27, 277, 431, 810
NcoI CCATGG 2 cut(s) 429, 616
NdeII GATC 6 cut(s) 899, 925, 949, 1045, 1160, 1276
NlaIII CATG 8 cut(s) 400, 433, 484, 572, 620, 937, 1036, 1074
NlaIV GGNNCC 4 cut(s) 435, 513, 869, 1239
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 2 cut(s) 105, 151
NsbI TGCGCA 1 cut(s) 573
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 2 cut(s) 572, 1036
PaeI GCATGC 1 cut(s) 572
PciI ACATGT 1 cut(s) 1032
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PctI GAATGC 1 cut(s) 747
PfeI GAWTC 3 cut(s) 446, 590, 740
PkrI GCNGC 6 cut(s) 23, 26, 29, 279, 597, 1266
PscI ACATGT 1 cut(s) 1032
PshBI ATTAAT 2 cut(s) 180, 630
PsiI TTATAA 1 cut(s) 456
Psp6I CCWGG 4 cut(s) 117, 636, 835, 1102
PspGI CCWGG 4 cut(s) 117, 636, 835, 1102
PspN4I GGNNCC 4 cut(s) 435, 513, 869, 1239
PspPI GGNCC 4 cut(s) 433, 497, 867, 1094
PsuI RGATCY 2 cut(s) 899, 925
RsaI GTAC 3 cut(s) 288, 390, 1040
RsaNI GTAC 3 cut(s) 287, 389, 1039
SaqAI TTAA 6 cut(s) 180, 192, 333, 585, 630, 759
SatI GCNGC 6 cut(s) 22, 25, 28, 278, 596, 1265
Sau3AI GATC 6 cut(s) 899, 925, 949, 1045, 1160, 1276
Sau96I GGNCC 4 cut(s) 433, 497, 867, 1094
ScrFI CCNGG 4 cut(s) 119, 638, 837, 1104
SduI GDGCHC 1 cut(s) 921
SfaNI GCATC 4 cut(s) 123, 173, 434, 661
SfcI CTRYAG 1 cut(s) 252
SinI GGWCC 1 cut(s) 1094
SmlI CTYRAG 1 cut(s) 438
SmoI CTYRAG 1 cut(s) 438
SpeI ACTAGT 1 cut(s) 356
SphI GCATGC 1 cut(s) 572
Sse9I AATT 9 cut(s) 62, 67, 184, 242, 334, 527, 627, 1109, 1149
SsiI CCGC 5 cut(s) 8, 269, 275, 278, 892
SspMI CTAG 3 cut(s) 36, 357, 1282
StyD4I CCNGG 4 cut(s) 117, 636, 835, 1102
StyI CCWWGG 2 cut(s) 429, 616
TaaI ACNGT 2 cut(s) 291, 461
TaiI ACGT 1 cut(s) 75
TaqI TCGA 6 cut(s) 534, 622, 799, 819, 1002, 1085
TasI AATT 9 cut(s) 62, 67, 184, 242, 334, 527, 627, 1109, 1149
TatI WGTACW 1 cut(s) 388
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 3 cut(s) 446, 590, 740
Tru1I TTAA 6 cut(s) 180, 192, 333, 585, 630, 759
Tru9I TTAA 6 cut(s) 180, 192, 333, 585, 630, 759
TscAI CASTG 3 cut(s) 37, 1172, 1266
TseFI GTSAC 2 cut(s) 105, 151
TseI GCWGC 5 cut(s) 21, 24, 27, 595, 1264
Tsp45I GTSAC 2 cut(s) 105, 151
TspDTI ATGAA 3 cut(s) 469, 603, 1182
TspRI CASTG 3 cut(s) 37, 1172, 1266
VneI GTGCAC 1 cut(s) 917
VpaK11BI GGWCC 1 cut(s) 1094
VspI ATTAAT 2 cut(s) 180, 630
XagI CCTNNNNNAGG 1 cut(s) 840
XapI RAATTY 3 cut(s) 527, 1109, 1149
XceI RCATGY 2 cut(s) 572, 1036
XspI CTAG 3 cut(s) 36, 357, 1282
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.