MD17G1247300.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008998
Physical Location & Seq
Forward (+)
29632164 .. 29634267
2104 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1247300.v1.1.491

Sequence Viewer

Length: 1236 bp
ATGACGAGCCATGAGCAATTCCAAATTCAATCAATTTTCCGGCCCTTCCGAACCCTAATCCAGCTAAACCATGTCCTTCATTTCCTTTTCTTTGGTATAGGTTTGTCACTAGGTATCATAATTACCTTATATTTCCAGAGCTTCTCATTCACTACTTTTCAAGCCTCCCTAAATTCCATTAACAATATTTTTCCTTTACAACGATCATCACCTCAAGCTCAATCGTCGCCACCCCCATCACCAAATCCGCTACCCCCACTGCATCCATTTCTCATACCATCTTTCTCCAAAAATGGGACATCTAACAGTGTTTCACTCAAAGAACAAAAATTCCTTGTACACAATATGAGTGACGAGGAGTTGTTTTCGAGGGCATTGATGGTTCCTCGAATTCAAGATTTGCCTTACAAGCTTGTTCCGAAAGTGGCTTTCATGTTCTTGACAAAGGGACCTCTGCCTTTGTCTCCCTTGTGGGAGAAGTTTTTCGAGGGACACGAAGGACTATACTCCATCTATGTGCACGCACACCCGGATTTCAATGAATCAGTGCCTGAAGATTCTGTGTTCCATAGTAGAAGAATTCATAGCCAGCCAGTCTATTGGGGAACATCATCAATGCTAGATGCCGAGAAACGACTCTTGGCAAATGCACTTCTTGACATCTCCAATCAAAGATTTGTGTTGCTTTCTGAATCCTGCATTCCTCTATTCAACTTCACAACAGCATATCACTACCTCATCAACTCAAACCTAAGCTTCCTGAACTCATTCGATGACCCGAGGAAGCCAGGCCGTGGCCGATACAACCCTCAAATGTACCCGATGATAAACATCACAGATTGGAGAAAGGGGTCTCAGTGGTTCGAAGTGCACCGTGACCTTGCCGTCCATATCGTTTCAGATCGGAAATATTATCCAATATTTCAACAGTACTGCAACCCTCCCTGTTACATTGATGAGCACTATATTCCAACACTAGTGAACTTGATGTACACTGAGTTGAATTCAAATAGGAGCGTCACTTGGGTGGATTGGTCAAGGGGTGGTCCTCATCCCGGAAAATTTGGATGGATGGATATTACGGATGAGTTTCTTAATCGGATTCGGTTCGGATCAGAATGCACGTACAATGGCAATACAACTTCAGTGTGTTTCTTGTTTGCTAGAAAATTCTTACCTGACGCATTGGAGCCTTTGTTGCGGGTTGCCCCTTTGCTACTTGGTTTTGATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

412

Amino Acids

47.52

Weight (kDa)

7.26

Isoelectric Point (pI)

48.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 142 - 369 1.4e-84 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 884
AccB7I CCANNNNNTGG 1 cut(s) 794
AciI CCGC 2 cut(s) 248, 1201
AclWI GGATC 2 cut(s) 1120, 1223
AcoI YGGCCR 1 cut(s) 796
AcsI RAATTY 8 cut(s) 24, 172, 329, 390, 579, 1003, 1061, 1169
AcuI CTGAAG 2 cut(s) 573, 1128
AfaI GTAC 5 cut(s) 339, 818, 932, 992, 1127
AfiI CCNNNNNNNGG 3 cut(s) 294, 794, 1055
AgsI TTSAA 8 cut(s) 29, 161, 395, 538, 712, 926, 1003, 1008
AhlI ACTAGT 1 cut(s) 976
AjnI CCWGG 1 cut(s) 787
AjuI GAANNNNNNNTTGG 2 cut(s) 623, 655
AleI CACNNNNGTG 1 cut(s) 1025
AloI GAACNNNNNNTCC 2 cut(s) 315, 347
AluBI AGCT 5 cut(s) 64, 141, 218, 412, 756
AluI AGCT 5 cut(s) 64, 141, 218, 412, 756
Alw21I GWGCWC 3 cut(s) 522, 873, 963
Alw26I GTCTC 2 cut(s) 468, 858
Alw44I GTGCAC 2 cut(s) 518, 869
AlwI GGATC 2 cut(s) 1120, 1223
AlwNI CAGNNNCTG 1 cut(s) 551
Ama87I CYCGRG 1 cut(s) 778
AoxI GGCC 3 cut(s) 41, 790, 796
ApaLI GTGCAC 2 cut(s) 518, 869
ApoI RAATTY 8 cut(s) 24, 172, 329, 390, 579, 1003, 1061, 1169
Asp700I GAANNNNTTC 2 cut(s) 482, 767
AspS9I GGNCC 3 cut(s) 42, 449, 1046
AsuC2I CCSGG 2 cut(s) 530, 1056
AsuHPI GGTGA 2 cut(s) 201, 231
AsuII TTCGAA 1 cut(s) 864
AvaI CYCGRG 1 cut(s) 778
AvaII GGWCC 2 cut(s) 449, 1046
BaeGI GKGCMC 2 cut(s) 522, 873
Bbv12I GWGCWC 3 cut(s) 522, 873, 963
BccI CCATC 6 cut(s) 244, 286, 373, 518, 1062, 1066
BceAI ACGGC 2 cut(s) 777, 869
BciT130I CCWGG 1 cut(s) 789
BcnI CCSGG 2 cut(s) 530, 1056
BcoDI GTCTC 2 cut(s) 468, 858
BcuI ACTAGT 1 cut(s) 976
BfaI CTAG 4 cut(s) 110, 620, 977, 1164
BmcAI AGTACT 1 cut(s) 932
Bme1390I CCNGG 3 cut(s) 530, 789, 1056
Bme18I GGWCC 2 cut(s) 449, 1046
BmeT110I CYCGRG 1 cut(s) 778
BmgT120I GGNCC 3 cut(s) 42, 449, 1046
BmiI GGNNCC 3 cut(s) 384, 450, 1191
BmrFI CCNGG 3 cut(s) 530, 789, 1056
BmsI GCATC 2 cut(s) 271, 613
Bpu10I CCTNAGC 1 cut(s) 752
Bpu14I TTCGAA 1 cut(s) 864
BpuEI CTTGAG 1 cut(s) 198
BpuMI CCSGG 2 cut(s) 530, 1056
BsaAI YACGTR 1 cut(s) 1125
BsaBI GATNNNNATC 1 cut(s) 830
BsaI GGTCTC 1 cut(s) 858
BsaJI CCNNGG 2 cut(s) 779, 793
BsaXI ACNNNNNCTCC 2 cut(s) 1181, 1211
Bsc4I CCNNNNNNNGG 3 cut(s) 294, 794, 1055
Bse1I ACTGG 1 cut(s) 593
Bse8I GATNNNNATC 1 cut(s) 830
BseBI CCWGG 1 cut(s) 789
BseDI CCNNGG 2 cut(s) 779, 793
BseGI GGATG 5 cut(s) 262, 1051, 1073, 1077, 1090
BseJI GATNNNNATC 1 cut(s) 830
BseLI CCNNNNNNNGG 3 cut(s) 294, 794, 1055
BseMII CTCAG 2 cut(s) 869, 987
BseNI ACTGG 1 cut(s) 593
BseRI GAGGAG 1 cut(s) 371
BseSI GKGCMC 2 cut(s) 522, 873
BshFI GGCC 3 cut(s) 43, 792, 798
BsiHKAI GWGCWC 3 cut(s) 522, 873, 963
BsiHKCI CYCGRG 1 cut(s) 778
BsiSI CCGG 3 cut(s) 40, 530, 1056
BslFI GGGAC 3 cut(s) 310, 462, 504
BslI CCNNNNNNNGG 3 cut(s) 294, 794, 1055
BsmAI GTCTC 2 cut(s) 468, 858
BsmFI GGGAC 3 cut(s) 310, 462, 504
BsmI GAATGC 2 cut(s) 699, 1124
BsnI GGCC 3 cut(s) 43, 792, 798
Bso31I GGTCTC 1 cut(s) 858
BsoBI CYCGRG 1 cut(s) 778
Bsp119I TTCGAA 1 cut(s) 864
Bsp1286I GDGCHC 3 cut(s) 522, 873, 963
Bsp1407I TGTACA 2 cut(s) 337, 990
Bsp143I GATC 4 cut(s) 203, 901, 1112, 1228
BspACI CCGC 2 cut(s) 248, 1201
BspANI GGCC 3 cut(s) 43, 792, 798
BspCNI CTCAG 2 cut(s) 868, 988
BspLI GGNNCC 3 cut(s) 384, 450, 1191
BspPI GGATC 2 cut(s) 1120, 1223
BspT104I TTCGAA 1 cut(s) 864
BspTNI GGTCTC 1 cut(s) 858
BsrGI TGTACA 2 cut(s) 337, 990
BsrI ACTGG 1 cut(s) 593
BssECI CCNNGG 2 cut(s) 779, 793
BssMI GATC 4 cut(s) 203, 901, 1112, 1228
Bst2UI CCWGG 1 cut(s) 789
Bst4CI ACNGT 3 cut(s) 308, 875, 930
BstAUI TGTACA 2 cut(s) 337, 990
BstBAI YACGTR 1 cut(s) 1125
BstBI TTCGAA 1 cut(s) 864
BstC8I GCNNGC 2 cut(s) 522, 590
BstDEI CTNAG 3 cut(s) 752, 855, 996
BstDSI CCRYGG 1 cut(s) 793
BstF5I GGATG 5 cut(s) 262, 1051, 1073, 1077, 1090
BstKTI GATC 4 cut(s) 206, 904, 1115, 1231
BstMAI GTCTC 2 cut(s) 468, 858
BstMBI GATC 4 cut(s) 203, 901, 1112, 1228
BstMWI GCNNNNNNNGC 2 cut(s) 409, 1198
BstNI CCWGG 1 cut(s) 789
BstSCI CCNGG 3 cut(s) 528, 787, 1054
BstSLI GKGCMC 2 cut(s) 522, 873
BstXI CCANNNNNNTGG 1 cut(s) 600
BsuRI GGCC 3 cut(s) 43, 792, 798
BtgI CCRYGG 1 cut(s) 793
BtsCI GGATG 5 cut(s) 262, 1051, 1073, 1077, 1090
BtsI GCAGTG 1 cut(s) 257
BtsIMutI CAGTG 6 cut(s) 257, 313, 552, 863, 993, 1152
Cac8I GCNNGC 2 cut(s) 522, 590
CaiI CAGNNNCTG 1 cut(s) 551
Cfr13I GGNCC 3 cut(s) 42, 449, 1046
CseI GACGC 2 cut(s) 1006, 1190
Csp6I GTAC 5 cut(s) 338, 817, 931, 991, 1126
CviAII CATG 3 cut(s) 11, 71, 433
CviQI GTAC 5 cut(s) 338, 817, 931, 991, 1126
DdeI CTNAG 3 cut(s) 752, 855, 996
DpnI GATC 4 cut(s) 205, 903, 1114, 1230
DpnII GATC 4 cut(s) 203, 901, 1112, 1228
DrdI GACNNNNNNGTC 1 cut(s) 884
DseDI GACNNNNNNGTC 1 cut(s) 884
EaeI YGGCCR 1 cut(s) 796
Eco31I GGTCTC 1 cut(s) 858
Eco47I GGWCC 2 cut(s) 449, 1046
Eco57I CTGAAG 2 cut(s) 573, 1128
Eco88I CYCGRG 1 cut(s) 778
EcoO109I RGGNCCY 1 cut(s) 449
EcoRI GAATTC 3 cut(s) 390, 579, 1003
EcoRII CCWGG 1 cut(s) 787
FaeI CATG 3 cut(s) 14, 74, 436
FaqI GGGAC 3 cut(s) 310, 462, 504
FatI CATG 3 cut(s) 10, 70, 432
FauI CCCGC 1 cut(s) 1194
FokI GGATG 5 cut(s) 249, 1038, 1080, 1084, 1097
FspBI CTAG 4 cut(s) 110, 620, 977, 1164
HaeIII GGCC 3 cut(s) 43, 792, 798
HapII CCGG 3 cut(s) 40, 530, 1056
HgaI GACGC 2 cut(s) 1006, 1190
Hin1II CATG 3 cut(s) 14, 74, 436
HindIII AAGCTT 2 cut(s) 410, 754
HinfI GANTC 5 cut(s) 542, 557, 636, 692, 1102
HpaII CCGG 3 cut(s) 40, 530, 1056
HphI GGTGA 2 cut(s) 201, 231
Hpy166II GTNNAC 5 cut(s) 340, 520, 871, 982, 993
Hpy188I TCNGA 8 cut(s) 50, 420, 691, 901, 906, 1101, 1112, 1117
Hpy188III TCNNGA 5 cut(s) 136, 395, 439, 656, 760
Hpy8I GTNNAC 5 cut(s) 340, 520, 871, 982, 993
Hpy99I CGWCG 1 cut(s) 229
HpyAV CCTTC 3 cut(s) 55, 86, 491
HpyCH4III ACNGT 3 cut(s) 308, 875, 930
HpyCH4IV ACGT 1 cut(s) 1124
HpyCH4V TGCA 7 cut(s) 262, 520, 650, 699, 871, 936, 1122
HpyF10VI GCNNNNNNNGC 2 cut(s) 409, 1198
HpyF3I CTNAG 3 cut(s) 752, 855, 996
HpySE526I ACGT 1 cut(s) 1124
Hsp92II CATG 3 cut(s) 14, 74, 436
Kzo9I GATC 4 cut(s) 203, 901, 1112, 1228
LmnI GCTCC 2 cut(s) 1014, 1189
LweI GCATC 2 cut(s) 271, 613
MaeI CTAG 4 cut(s) 110, 620, 977, 1164
MaeII ACGT 1 cut(s) 1124
MaeIII GTNAC 5 cut(s) 105, 350, 875, 947, 1018
MalI GATC 4 cut(s) 205, 903, 1114, 1230
MboI GATC 4 cut(s) 203, 901, 1112, 1228
MboII GAAGA 2 cut(s) 566, 588
MhlI GDGCHC 3 cut(s) 522, 873, 963
MlyI GAGTC 1 cut(s) 630
MmeI TCCRAC 1 cut(s) 995
MroXI GAANNNNTTC 2 cut(s) 482, 767
MseI TTAA 2 cut(s) 180, 1095
MslI CAYNNNNRTG 2 cut(s) 515, 1025
MspI CCGG 3 cut(s) 40, 530, 1056
MspR9I CCNGG 3 cut(s) 530, 789, 1056
Mva1269I GAATGC 2 cut(s) 699, 1124
MvaI CCWGG 1 cut(s) 789
MwoI GCNNNNNNNGC 2 cut(s) 409, 1198
NciI CCSGG 2 cut(s) 530, 1056
NdeII GATC 4 cut(s) 203, 901, 1112, 1228
NlaIII CATG 3 cut(s) 14, 74, 436
NlaIV GGNNCC 3 cut(s) 384, 450, 1191
NmeAIII GCCGAG 1 cut(s) 652
NmuCI GTSAC 4 cut(s) 105, 350, 875, 1018
NspV TTCGAA 1 cut(s) 864
OliI CACNNNNGTG 1 cut(s) 1025
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PctI GAATGC 2 cut(s) 699, 1124
PdmI GAANNNNTTC 2 cut(s) 482, 767
PfeI GAWTC 4 cut(s) 542, 557, 692, 1102
PflMI CCANNNNNTGG 1 cut(s) 794
PfoI TCCNGGA 1 cut(s) 1054
PleI GAGTC 1 cut(s) 630
PpsI GAGTC 1 cut(s) 630
Ppu21I YACGTR 1 cut(s) 1125
PpuMI RGGWCCY 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 787
PspGI CCWGG 1 cut(s) 787
PspN4I GGNNCC 3 cut(s) 384, 450, 1191
PspPI GGNCC 3 cut(s) 42, 449, 1046
PspPPI RGGWCCY 1 cut(s) 449
PsrI GAACNNNNNNTAC 2 cut(s) 974, 1006
PstNI CAGNNNCTG 1 cut(s) 551
RsaI GTAC 5 cut(s) 339, 818, 932, 992, 1127
RsaNI GTAC 5 cut(s) 338, 817, 931, 991, 1126
RseI CAYNNNNRTG 2 cut(s) 515, 1025
SaqAI TTAA 2 cut(s) 180, 1095
Sau3AI GATC 4 cut(s) 203, 901, 1112, 1228
Sau96I GGNCC 3 cut(s) 42, 449, 1046
ScaI AGTACT 1 cut(s) 932
SchI GAGTC 1 cut(s) 630
ScrFI CCNGG 3 cut(s) 530, 789, 1056
SduI GDGCHC 3 cut(s) 522, 873, 963
SfaNI GCATC 2 cut(s) 271, 613
SfuI TTCGAA 1 cut(s) 864
SinI GGWCC 2 cut(s) 449, 1046
SmiMI CAYNNNNRTG 2 cut(s) 515, 1025
SmlI CTYRAG 1 cut(s) 213
SmoI CTYRAG 1 cut(s) 213
SpeI ACTAGT 1 cut(s) 976
SsiI CCGC 2 cut(s) 248, 1201
SspI AATATT 3 cut(s) 187, 911, 921
SspMI CTAG 4 cut(s) 110, 620, 977, 1164
StyD4I CCNGG 3 cut(s) 528, 787, 1054
TaaI ACNGT 3 cut(s) 308, 875, 930
TaiI ACGT 1 cut(s) 1127
TaqI TCGA 5 cut(s) 368, 388, 486, 771, 864
TatI WGTACW 3 cut(s) 337, 930, 990
TfiI GAWTC 4 cut(s) 542, 557, 692, 1102
Tru1I TTAA 2 cut(s) 180, 1095
Tru9I TTAA 2 cut(s) 180, 1095
TscAI CASTG 6 cut(s) 264, 313, 552, 863, 1000, 1152
TseFI GTSAC 4 cut(s) 105, 350, 875, 1018
Tsp45I GTSAC 4 cut(s) 105, 350, 875, 1018
TspDTI ATGAA 4 cut(s) 68, 421, 555, 572
TspGWI ACGGA 1 cut(s) 1097
TspRI CASTG 6 cut(s) 264, 313, 552, 863, 1000, 1152
Van91I CCANNNNNTGG 1 cut(s) 794
VneI GTGCAC 2 cut(s) 518, 869
VpaK11BI GGWCC 2 cut(s) 449, 1046
XapI RAATTY 8 cut(s) 24, 172, 329, 390, 579, 1003, 1061, 1169
XmnI GAANNNNTTC 2 cut(s) 482, 767
XspI CTAG 4 cut(s) 110, 620, 977, 1164
ZrmI AGTACT 1 cut(s) 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.