pycom17g24870

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008998
Physical Location & Seq
Forward (+)
23100661 .. 23102489
1829 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g24870.1

Sequence Viewer

Length: 969 bp
ATGACGAGCCATGAGCAATTCCAAATTCAATCAATTTTCCGGCCCTTTCGAACCCTAATCCAGCTAAACCATGTCCTTCATTTCTTTTTCTTTGGTATAGGTTTGTCACTAGGTATCATAATTACCTTATATTTCCAGAGCTTCTCATTCACTACTTTTCAAGCCTCCCTAAATTCCATTAACAATATTTTTCCTTTACAACCATCATCACTTCAAGCTCAATCGTCGCCACCCCCATCACCAAATCCGCTTCCCACACTGCCGCCATTTCTCATACTATCTTTCTCCAAAAATGGGACATCTAACAATGTTTCACTCACAGAACAAAAATTCCTTGTACACAACAAGAGTGACAAGGAGTTGTTTTCGAGGGCATTGATGGTTCCTCGAATTCAAGATTTGCCTTACAAGCTTGTTCCGAAAGTGGCTTTCATGTTCTTGACAAAGGGACCTTTGCCTTTGTCTCCCTTGTGGGAGAAGTTTTTCGAGGGACACGAAGGACTATACTCCATCTATGTGCACGCACACCCCGATTTCAATGAATCGGTGCCTGAAGAGTCTGTGTTCCATGGTAGAAGAATTCATAGCCAGCCAGTCTATTGGGGGACATCATCAATGCTAGATGCCGAGAGACGACTCCTGGCAAATGCACTTCTTGACATCTCCAATCAAAGATTTGTGTTGCTTTGTGAATCCTGCATTCCTCTATTCAACTTCACAACAGCATATCACTACCTCATCAACTCAAACCTAAGCTTCCTGAACTCATTCGATGACCCGAGGAAGCCAGGCCGTGGCCGATACAACCCTCAAATGTACCCCATGATAAACATCACAGATTGGAGAAAGGGGTCTCAGTGGTTCGAAGTGCACCGTGAGCTTGCCGTCCACATCGTTTCAGATCGATCTTTCAACAGTACTGCAACCCTCCCTGTTACATTGATGAGCACTATATTCCAACACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

323

Amino Acids

37.03

Weight (kDa)

8.83

Isoelectric Point (pI)

52.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 142 - 303 2.5e-59 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 547
AccB7I CCANNNNNTGG 1 cut(s) 794
AciI CCGC 2 cut(s) 248, 263
AcoI YGGCCR 1 cut(s) 796
AcsI RAATTY 5 cut(s) 24, 172, 329, 390, 579
AcuI CTGAAG 1 cut(s) 573
AfaI GTAC 3 cut(s) 339, 818, 919
AfiI CCNNNNNNNGG 2 cut(s) 294, 794
AgsI TTSAA 7 cut(s) 29, 161, 215, 395, 538, 712, 913
AjnI CCWGG 2 cut(s) 639, 787
AloI GAACNNNNNNTCC 2 cut(s) 315, 347
AluBI AGCT 6 cut(s) 64, 141, 218, 412, 756, 880
AluI AGCT 6 cut(s) 64, 141, 218, 412, 756, 880
Alw21I GWGCWC 3 cut(s) 522, 873, 950
Alw26I GTCTC 3 cut(s) 468, 625, 858
Alw44I GTGCAC 2 cut(s) 518, 869
Ama87I CYCGRG 1 cut(s) 778
AoxI GGCC 3 cut(s) 41, 790, 796
ApaLI GTGCAC 2 cut(s) 518, 869
ApoI RAATTY 5 cut(s) 24, 172, 329, 390, 579
Asp700I GAANNNNTTC 2 cut(s) 482, 767
AspS9I GGNCC 2 cut(s) 42, 449
AsuHPI GGTGA 1 cut(s) 231
AsuII TTCGAA 2 cut(s) 49, 864
AvaI CYCGRG 1 cut(s) 778
AvaII GGWCC 1 cut(s) 449
BaeGI GKGCMC 2 cut(s) 522, 873
BanI GGYRCC 1 cut(s) 547
Bbv12I GWGCWC 3 cut(s) 522, 873, 950
BccI CCATC 4 cut(s) 211, 244, 373, 518
BceAI ACGGC 2 cut(s) 777, 869
BciT130I CCWGG 2 cut(s) 641, 789
BcoDI GTCTC 3 cut(s) 468, 625, 858
BfaI CTAG 2 cut(s) 110, 620
BisI GCNGC 1 cut(s) 263
BlsI GCNGC 1 cut(s) 264
BmcAI AGTACT 1 cut(s) 919
Bme1390I CCNGG 2 cut(s) 641, 789
Bme18I GGWCC 1 cut(s) 449
BmeT110I CYCGRG 1 cut(s) 778
BmgT120I GGNCC 2 cut(s) 42, 449
BmiI GGNNCC 3 cut(s) 384, 450, 549
BmrFI CCNGG 2 cut(s) 641, 789
BmsI GCATC 1 cut(s) 613
Bpu10I CCTNAGC 1 cut(s) 752
Bpu14I TTCGAA 2 cut(s) 49, 864
Bsa29I ATCGAT 1 cut(s) 904
BsaBI GATNNNNATC 1 cut(s) 830
BsaI GGTCTC 1 cut(s) 858
BsaJI CCNNGG 3 cut(s) 568, 779, 793
Bsc4I CCNNNNNNNGG 2 cut(s) 294, 794
Bse1I ACTGG 1 cut(s) 593
Bse8I GATNNNNATC 1 cut(s) 830
BseBI CCWGG 2 cut(s) 641, 789
BseCI ATCGAT 1 cut(s) 904
BseDI CCNNGG 3 cut(s) 568, 779, 793
BseJI GATNNNNATC 1 cut(s) 830
BseLI CCNNNNNNNGG 2 cut(s) 294, 794
BseMII CTCAG 1 cut(s) 869
BseNI ACTGG 1 cut(s) 593
BseSI GKGCMC 2 cut(s) 522, 873
BshFI GGCC 3 cut(s) 43, 792, 798
BshNI GGYRCC 1 cut(s) 547
BshVI ATCGAT 1 cut(s) 904
BsiHKAI GWGCWC 3 cut(s) 522, 873, 950
BsiHKCI CYCGRG 1 cut(s) 778
BsiSI CCGG 1 cut(s) 40
BslFI GGGAC 4 cut(s) 310, 462, 504, 619
BslI CCNNNNNNNGG 2 cut(s) 294, 794
BsmAI GTCTC 3 cut(s) 468, 625, 858
BsmBI CGTCTC 1 cut(s) 625
BsmFI GGGAC 4 cut(s) 310, 462, 504, 619
BsmI GAATGC 1 cut(s) 699
BsnI GGCC 3 cut(s) 43, 792, 798
Bso31I GGTCTC 1 cut(s) 858
BsoBI CYCGRG 1 cut(s) 778
Bsp119I TTCGAA 2 cut(s) 49, 864
Bsp1286I GDGCHC 3 cut(s) 522, 873, 950
Bsp1407I TGTACA 1 cut(s) 337
Bsp143I GATC 2 cut(s) 901, 905
Bsp19I CCATGG 1 cut(s) 568
BspACI CCGC 2 cut(s) 248, 263
BspANI GGCC 3 cut(s) 43, 792, 798
BspCNI CTCAG 1 cut(s) 868
BspDI ATCGAT 1 cut(s) 904
BspLI GGNNCC 3 cut(s) 384, 450, 549
BspT104I TTCGAA 2 cut(s) 49, 864
BspT107I GGYRCC 1 cut(s) 547
BspTNI GGTCTC 1 cut(s) 858
BsrGI TGTACA 1 cut(s) 337
BsrI ACTGG 1 cut(s) 593
BssECI CCNNGG 3 cut(s) 568, 779, 793
BssMI GATC 2 cut(s) 901, 905
BssT1I CCWWGG 1 cut(s) 568
Bst2UI CCWGG 2 cut(s) 641, 789
Bst4CI ACNGT 2 cut(s) 875, 917
Bst6I CTCTTC 1 cut(s) 549
BstAUI TGTACA 1 cut(s) 337
BstBI TTCGAA 2 cut(s) 49, 864
BstC8I GCNNGC 3 cut(s) 522, 590, 882
BstDEI CTNAG 2 cut(s) 752, 855
BstDSI CCRYGG 2 cut(s) 568, 793
BstKTI GATC 2 cut(s) 904, 908
BstMAI GTCTC 3 cut(s) 468, 625, 858
BstMBI GATC 2 cut(s) 901, 905
BstMWI GCNNNNNNNGC 2 cut(s) 409, 877
BstNI CCWGG 2 cut(s) 641, 789
BstSCI CCNGG 2 cut(s) 639, 787
BstSLI GKGCMC 2 cut(s) 522, 873
BstXI CCANNNNNNTGG 1 cut(s) 600
Bsu15I ATCGAT 1 cut(s) 904
BsuRI GGCC 3 cut(s) 43, 792, 798
BsuTUI ATCGAT 1 cut(s) 904
BtgI CCRYGG 2 cut(s) 568, 793
BtsI GCAGTG 1 cut(s) 257
BtsIMutI CAGTG 2 cut(s) 257, 863
Cac8I GCNNGC 3 cut(s) 522, 590, 882
Cfr13I GGNCC 2 cut(s) 42, 449
ClaI ATCGAT 1 cut(s) 904
Csp6I GTAC 3 cut(s) 338, 817, 918
CviAII CATG 5 cut(s) 11, 71, 433, 569, 823
CviQI GTAC 3 cut(s) 338, 817, 918
DdeI CTNAG 2 cut(s) 752, 855
DpnI GATC 2 cut(s) 903, 907
DpnII GATC 2 cut(s) 901, 905
EaeI YGGCCR 1 cut(s) 796
Eam1104I CTCTTC 1 cut(s) 549
EarI CTCTTC 1 cut(s) 549
Eco130I CCWWGG 1 cut(s) 568
Eco31I GGTCTC 1 cut(s) 858
Eco47I GGWCC 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 573
Eco88I CYCGRG 1 cut(s) 778
EcoO109I RGGNCCY 1 cut(s) 449
EcoRI GAATTC 2 cut(s) 390, 579
EcoRII CCWGG 2 cut(s) 639, 787
EcoT14I CCWWGG 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 568
Esp3I CGTCTC 1 cut(s) 625
FaeI CATG 5 cut(s) 14, 74, 436, 572, 826
FaqI GGGAC 4 cut(s) 310, 462, 504, 619
FatI CATG 5 cut(s) 10, 70, 432, 568, 822
Fnu4HI GCNGC 1 cut(s) 263
Fsp4HI GCNGC 1 cut(s) 263
FspBI CTAG 2 cut(s) 110, 620
GluI GCNGC 1 cut(s) 263
HaeIII GGCC 3 cut(s) 43, 792, 798
HapII CCGG 1 cut(s) 40
Hin1II CATG 5 cut(s) 14, 74, 436, 572, 826
HindIII AAGCTT 2 cut(s) 410, 754
HinfI GANTC 4 cut(s) 542, 557, 636, 692
HpaII CCGG 1 cut(s) 40
HphI GGTGA 1 cut(s) 231
Hpy166II GTNNAC 4 cut(s) 340, 520, 871, 889
Hpy188I TCNGA 2 cut(s) 420, 901
Hpy188III TCNNGA 5 cut(s) 136, 395, 439, 656, 760
Hpy8I GTNNAC 4 cut(s) 340, 520, 871, 889
Hpy99I CGWCG 1 cut(s) 229
HpyAV CCTTC 2 cut(s) 86, 491
HpyCH4III ACNGT 2 cut(s) 875, 917
HpyCH4V TGCA 5 cut(s) 520, 650, 699, 871, 923
HpyF10VI GCNNNNNNNGC 2 cut(s) 409, 877
HpyF3I CTNAG 2 cut(s) 752, 855
Hsp92II CATG 5 cut(s) 14, 74, 436, 572, 826
Kzo9I GATC 2 cut(s) 901, 905
LweI GCATC 1 cut(s) 613
MaeI CTAG 2 cut(s) 110, 620
MaeIII GTNAC 3 cut(s) 105, 350, 934
MalI GATC 2 cut(s) 903, 907
MboI GATC 2 cut(s) 901, 905
MboII GAAGA 2 cut(s) 566, 588
MhlI GDGCHC 3 cut(s) 522, 873, 950
MluCI AATT 8 cut(s) 17, 24, 33, 120, 172, 329, 390, 579
MlyI GAGTC 2 cut(s) 566, 630
MnlI CCTC 9 cut(s) 175, 363, 396, 481, 714, 746, 774, 819, 938
MroXI GAANNNNTTC 2 cut(s) 482, 767
MseI TTAA 1 cut(s) 180
MslI CAYNNNNRTG 1 cut(s) 515
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 2 cut(s) 641, 789
Mva1269I GAATGC 1 cut(s) 699
MvaI CCWGG 2 cut(s) 641, 789
MwoI GCNNNNNNNGC 2 cut(s) 409, 877
NcoI CCATGG 1 cut(s) 568
NdeII GATC 2 cut(s) 901, 905
NlaIII CATG 5 cut(s) 14, 74, 436, 572, 826
NlaIV GGNNCC 3 cut(s) 384, 450, 549
NmeAIII GCCGAG 1 cut(s) 652
NmuCI GTSAC 2 cut(s) 105, 350
NspV TTCGAA 2 cut(s) 49, 864
PcsI WCGNNNNNNNCGW 2 cut(s) 492, 528
PctI GAATGC 1 cut(s) 699
PdmI GAANNNNTTC 2 cut(s) 482, 767
PfeI GAWTC 2 cut(s) 542, 692
PflMI CCANNNNNTGG 1 cut(s) 794
PkrI GCNGC 1 cut(s) 264
PleI GAGTC 2 cut(s) 565, 630
PpsI GAGTC 2 cut(s) 565, 630
PpuMI RGGWCCY 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 639, 787
PspGI CCWGG 2 cut(s) 639, 787
PspN4I GGNNCC 3 cut(s) 384, 450, 549
PspPI GGNCC 2 cut(s) 42, 449
PspPPI RGGWCCY 1 cut(s) 449
RsaI GTAC 3 cut(s) 339, 818, 919
RsaNI GTAC 3 cut(s) 338, 817, 918
RseI CAYNNNNRTG 1 cut(s) 515
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 1 cut(s) 263
Sau3AI GATC 2 cut(s) 901, 905
Sau96I GGNCC 2 cut(s) 42, 449
ScaI AGTACT 1 cut(s) 919
SchI GAGTC 2 cut(s) 566, 630
ScrFI CCNGG 2 cut(s) 641, 789
SduI GDGCHC 3 cut(s) 522, 873, 950
SfaNI GCATC 1 cut(s) 613
SfuI TTCGAA 2 cut(s) 49, 864
SinI GGWCC 1 cut(s) 449
SmiMI CAYNNNNRTG 1 cut(s) 515
Sse9I AATT 8 cut(s) 17, 24, 33, 120, 172, 329, 390, 579
SsiI CCGC 2 cut(s) 248, 263
SspI AATATT 1 cut(s) 187
SspMI CTAG 2 cut(s) 110, 620
StyD4I CCNGG 2 cut(s) 639, 787
StyI CCWWGG 1 cut(s) 568
TaaI ACNGT 2 cut(s) 875, 917
TaqI TCGA 7 cut(s) 49, 368, 388, 486, 771, 864, 904
TasI AATT 8 cut(s) 17, 24, 33, 120, 172, 329, 390, 579
TatI WGTACW 2 cut(s) 337, 917
TauI GCSGC 1 cut(s) 265
TfiI GAWTC 2 cut(s) 542, 692
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 2 cut(s) 264, 863
TseFI GTSAC 2 cut(s) 105, 350
Tsp45I GTSAC 2 cut(s) 105, 350
TspDTI ATGAA 4 cut(s) 68, 421, 555, 572
TspRI CASTG 2 cut(s) 264, 863
Van91I CCANNNNNTGG 1 cut(s) 794
VneI GTGCAC 2 cut(s) 518, 869
VpaK11BI GGWCC 1 cut(s) 449
XapI RAATTY 5 cut(s) 24, 172, 329, 390, 579
XmnI GAANNNNTTC 2 cut(s) 482, 767
XspI CTAG 2 cut(s) 110, 620
ZrmI AGTACT 1 cut(s) 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.