Rh3BG349700

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0008998
Physical Location & Seq
Reverse (-)
39104904 .. 39105659
756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG349700.1

Sequence Viewer

Length: 525 bp
ATGAGCGACGAGGAGTTACTTTGGAGAGCATCCATGGCCCCTCAAGTTGATTCGCCTTATAACTGTGTTCCGAAAGTGGCTTTCATGTTCTTGACAAAAGGGCCTCTACCTTTGGCTCCCTTATGGGACAAATTTTTTGAGGGACACGAGGGTCTATACTCTATCTATGTGCACGCGCACCCTTCTTTTAATGAATCGCAGCCTCAAACTTCTGTCTTCCATGGTCGAAGAATTAATAGCCAGGCCGTTTACTGGGGAACTTCCTCAATGTTGGATGCAGAGCAACGGCTATTAGCAAATGCACTGCTTGACTTGTCAAATCAAAGATTTGTATTGCTTTCCGAATCGTGCATTCCCTTGTTTAACTTCACGACAACTTATGACTACCTTGTAAACTCAAATCAAAGCTTTCTAAGCGTATTCGACGACCCGAGGAAGCCTGGTCGAGGGCGCTACAACCCTCAAATGTGGCCCCAAATAAACATCACAGATTGGCGGAAAGGATCTCAATGGTTTGAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

174

Amino Acids

20.15

Weight (kDa)

5.76

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 26 - 174 3.5e-53 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 60
AasI GACNNNNNNGTC 1 cut(s) 150
AccII CGCG 1 cut(s) 176
AciI CCGC 1 cut(s) 496
AclWI GGATC 1 cut(s) 511
AcsI RAATTY 1 cut(s) 131
AfiI CCNNNNNNNGG 2 cut(s) 252, 446
AgsI TTSAA 1 cut(s) 518
AjnI CCWGG 2 cut(s) 240, 439
AluBI AGCT 1 cut(s) 408
AluI AGCT 1 cut(s) 408
Alw21I GWGCWC 1 cut(s) 174
Alw44I GTGCAC 1 cut(s) 170
AlwI GGATC 1 cut(s) 511
Ama87I CYCGRG 1 cut(s) 430
AoxI GGCC 4 cut(s) 36, 101, 243, 470
ApaLI GTGCAC 1 cut(s) 170
ApeKI GCWGC 1 cut(s) 199
ApoI RAATTY 1 cut(s) 131
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
AseI ATTAAT 1 cut(s) 234
AspLEI GCGC 2 cut(s) 178, 453
AspS9I GGNCC 3 cut(s) 37, 101, 471
AvaI CYCGRG 1 cut(s) 430
BaeGI GKGCMC 1 cut(s) 174
BauI CACGAG 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 211
BceAI ACGGC 2 cut(s) 230, 302
BciT130I CCWGG 2 cut(s) 242, 441
BfoI RGCGCY 1 cut(s) 454
BisI GCNGC 1 cut(s) 200
BlsI GCNGC 1 cut(s) 201
Bme1390I CCNGG 2 cut(s) 242, 441
BmeT110I CYCGRG 1 cut(s) 430
BmgT120I GGNCC 3 cut(s) 37, 101, 471
BmiI GGNNCC 3 cut(s) 39, 117, 473
BmrFI CCNGG 2 cut(s) 242, 441
BmrI ACTGGG 1 cut(s) 262
BmsI GCATC 2 cut(s) 38, 265
BmuI ACTGGG 1 cut(s) 262
BpiI GAAGAC 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 27
BsaJI CCNNGG 3 cut(s) 33, 220, 431
Bsc4I CCNNNNNNNGG 2 cut(s) 252, 446
Bse1I ACTGG 1 cut(s) 257
BseBI CCWGG 2 cut(s) 242, 441
BseDI CCNNGG 3 cut(s) 33, 220, 431
BseGI GGATG 2 cut(s) 29, 280
BseLI CCNNNNNNNGG 2 cut(s) 252, 446
BseNI ACTGG 1 cut(s) 257
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 1 cut(s) 211
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 4 cut(s) 38, 103, 245, 472
BsiHKAI GWGCWC 1 cut(s) 174
BsiHKCI CYCGRG 1 cut(s) 430
BslFI GGGAC 2 cut(s) 140, 156
BslI CCNNNNNNNGG 2 cut(s) 252, 446
BsmFI GGGAC 2 cut(s) 140, 156
BsmI GAATGC 1 cut(s) 351
BsnI GGCC 4 cut(s) 38, 103, 245, 472
BsoBI CYCGRG 1 cut(s) 430
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 1 cut(s) 503
Bsp19I CCATGG 2 cut(s) 33, 220
BspACI CCGC 1 cut(s) 496
BspANI GGCC 4 cut(s) 38, 103, 245, 472
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 3 cut(s) 39, 117, 473
BspPI GGATC 1 cut(s) 511
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 3 cut(s) 33, 220, 431
BssMI GATC 1 cut(s) 503
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 2 cut(s) 33, 220
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 2 cut(s) 242, 441
Bst4CI ACNGT 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 1 cut(s) 413
BstDSI CCRYGG 2 cut(s) 33, 220
BstENI CCTNNNNNAGG 1 cut(s) 444
BstF5I GGATG 2 cut(s) 29, 280
BstFNI CGCG 1 cut(s) 176
BstH2I RGCGCY 1 cut(s) 454
BstHHI GCGC 2 cut(s) 178, 453
BstKTI GATC 1 cut(s) 506
BstMBI GATC 1 cut(s) 503
BstMWI GCNNNNNNNGC 2 cut(s) 35, 414
BstNI CCWGG 2 cut(s) 242, 441
BstSCI CCNGG 2 cut(s) 240, 439
BstSLI GKGCMC 1 cut(s) 174
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 1 cut(s) 211
BstV2I GAAGAC 1 cut(s) 208
BstX2I RGATCY 1 cut(s) 503
BstYI RGATCY 1 cut(s) 503
BsuRI GGCC 4 cut(s) 38, 103, 245, 472
BtgI CCRYGG 2 cut(s) 33, 220
BtsCI GGATG 2 cut(s) 29, 280
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 1 cut(s) 302
Cac8I GCNNGC 1 cut(s) 174
CfoI GCGC 2 cut(s) 178, 453
Cfr13I GGNCC 3 cut(s) 37, 101, 471
CviAII CATG 3 cut(s) 34, 85, 221
DdeI CTNAG 1 cut(s) 413
DpnI GATC 1 cut(s) 505
DpnII GATC 1 cut(s) 503
DrdI GACNNNNNNGTC 1 cut(s) 150
DseDI GACNNNNNNGTC 1 cut(s) 150
EciI GGCGGA 1 cut(s) 511
Eco130I CCWWGG 2 cut(s) 33, 220
Eco88I CYCGRG 1 cut(s) 430
EcoNI CCTNNNNNAGG 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 101
EcoRII CCWGG 2 cut(s) 240, 439
EcoT14I CCWWGG 2 cut(s) 33, 220
ErhI CCWWGG 2 cut(s) 33, 220
FaeI CATG 3 cut(s) 37, 88, 224
FaiI YATR 8 cut(s) 35, 60, 86, 124, 157, 168, 222, 381
FaqI GGGAC 2 cut(s) 140, 156
FatI CATG 3 cut(s) 33, 84, 220
Fnu4HI GCNGC 1 cut(s) 200
FokI GGATG 2 cut(s) 16, 287
Fsp4HI GCNGC 1 cut(s) 200
GlaI GCGC 2 cut(s) 177, 452
GluI GCNGC 1 cut(s) 200
HaeII RGCGCY 1 cut(s) 454
HaeIII GGCC 4 cut(s) 38, 103, 245, 472
HhaI GCGC 2 cut(s) 178, 453
Hin1II CATG 3 cut(s) 37, 88, 224
Hin6I GCGC 2 cut(s) 176, 451
HinP1I GCGC 2 cut(s) 176, 451
HindIII AAGCTT 1 cut(s) 406
HinfI GANTC 3 cut(s) 50, 194, 344
Hpy166II GTNNAC 3 cut(s) 172, 250, 394
Hpy188I TCNGA 2 cut(s) 72, 343
Hpy188III TCNNGA 2 cut(s) 91, 370
Hpy8I GTNNAC 3 cut(s) 172, 250, 394
Hpy99I CGWCG 2 cut(s) 11, 428
HpyAV CCTTC 1 cut(s) 192
HpyCH4III ACNGT 1 cut(s) 65
HpyCH4V TGCA 4 cut(s) 172, 278, 302, 351
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 414
HpyF3I CTNAG 1 cut(s) 413
Hsp92II CATG 3 cut(s) 37, 88, 224
HspAI GCGC 2 cut(s) 176, 451
Kzo9I GATC 1 cut(s) 503
LmnI GCTCC 1 cut(s) 121
LpnPI CCDG 5 cut(s) 227, 238, 254, 426, 453
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 2 cut(s) 38, 265
MaeIII GTNAC 1 cut(s) 15
MalI GATC 1 cut(s) 505
MboI GATC 1 cut(s) 503
MboII GAAGA 2 cut(s) 208, 240
MflI RGATCY 1 cut(s) 503
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 2 cut(s) 131, 231
MmeI TCCRAC 1 cut(s) 252
MseI TTAA 3 cut(s) 189, 234, 363
MspR9I CCNGG 2 cut(s) 242, 441
Mva1269I GAATGC 1 cut(s) 351
MvaI CCWGG 2 cut(s) 242, 441
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 2 cut(s) 35, 414
NcoI CCATGG 2 cut(s) 33, 220
NdeII GATC 1 cut(s) 503
NlaIII CATG 3 cut(s) 37, 88, 224
NlaIV GGNNCC 3 cut(s) 39, 117, 473
PctI GAATGC 1 cut(s) 351
PfeI GAWTC 3 cut(s) 50, 194, 344
PkrI GCNGC 1 cut(s) 201
PshBI ATTAAT 1 cut(s) 234
PsiI TTATAA 1 cut(s) 60
Psp6I CCWGG 2 cut(s) 240, 439
PspGI CCWGG 2 cut(s) 240, 439
PspN4I GGNNCC 3 cut(s) 39, 117, 473
PspPI GGNCC 3 cut(s) 37, 101, 471
PsuI RGATCY 1 cut(s) 503
SaqAI TTAA 3 cut(s) 189, 234, 363
SatI GCNGC 1 cut(s) 200
Sau3AI GATC 1 cut(s) 503
Sau96I GGNCC 3 cut(s) 37, 101, 471
ScrFI CCNGG 2 cut(s) 242, 441
SduI GDGCHC 1 cut(s) 174
SetI ASST 3 cut(s) 112, 390, 410
SfaNI GCATC 2 cut(s) 38, 265
SmlI CTYRAG 1 cut(s) 42
SmoI CTYRAG 1 cut(s) 42
Sse9I AATT 2 cut(s) 131, 231
SsiI CCGC 1 cut(s) 496
StyD4I CCNGG 2 cut(s) 240, 439
StyI CCWWGG 2 cut(s) 33, 220
TaaI ACNGT 1 cut(s) 65
TaqI TCGA 3 cut(s) 226, 423, 445
TasI AATT 2 cut(s) 131, 231
TfiI GAWTC 3 cut(s) 50, 194, 344
Tru1I TTAA 3 cut(s) 189, 234, 363
Tru9I TTAA 3 cut(s) 189, 234, 363
TscAI CASTG 1 cut(s) 309
TseI GCWGC 1 cut(s) 199
TspDTI ATGAA 2 cut(s) 73, 207
TspRI CASTG 1 cut(s) 309
VneI GTGCAC 1 cut(s) 170
VspI ATTAAT 1 cut(s) 234
XagI CCTNNNNNAGG 1 cut(s) 444
XapI RAATTY 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.