RchiOBHm_Chr3g0494101

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0008998
Physical Location & Seq
Reverse (-)
41748992 .. 41750380
1389 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45670

Sequence Viewer

Length: 1290 bp
ATGATGGCAGCGATATTGTGCAGCAGCAGCAGTGCTAGTGAGCAACATCATCGGGCGTTTCAATTCAATTACGTCCTGCATTTCCTCTTCTTTGTTATTGGTTTGTCACTCGGCATCCTGGCTACCTTATATTTCCAGAGTTACTCATTAGTCACTTTTCAAGCATCTCTCAACTCCATTAATAATTTTATTAACACTCCGCTAAAACCATCACCCCCATCACAACCTCCGCCTCCACCGAAATTACCACCTACTCCTCCACCTCCACCGCCACCGCCACCGCCGCTTCTGGTACTGTCTTTCTCAACAATTAATGCAACATCATCATCAGTGTTAGTTAATTACAGTAGCAGTCTCAGCACTAGTGTTTCTTTGAAAGAACAAAAGCTGTTAGTACACAACATGAGCGACGAGGAGTTACTTTGGAGAGCATCCATGGCCCCTCAAGTTGATTCGCCTTATAACTGTGTTCCGAAAGTGGCTTTCATGTTCTTGACAAAAGGGCCTCTACCTTTGGCTCCCTTATGGGACAAATTTTTTGAGGGACACGAGGGTCTATACTCTATCTATGTGCATGCGCACCCTTCTTTTAATGAATCGCAGCCTCAAACTTCTGTCTTCCATGGTCGAAGAATTAATAGCCAGGCCGTTTACTGGGGAACTTCCTCAATGTTGGATGCAGAGCGACGGCTACTAGCAAATGCACTGCTTGACTTGTCAAATCAAAGATTTGTATTGCTTTCCGAATCGTGCATTCCCTTGTTTAACTTCACGACAACTTATGACTACCTTGTAAACTCAAATAAGAGCTTTCTAAGCGTATTCGATGACCCGAGGAAGCCTGGTCGAGGGCGCTACAACCCTCAAATGTGGCCCCAAATAAACATCACAGATTGGCGGAAAGGATCTCAATGGTTTGAAGTGCACCGAGACCTGGCCATCCACATTGTCTCCGATCAGAAATACTACCCCATCTTTCAACAATACTGCCACCCTCCTTGTTATATCGATGAGCATTATATTCCAACACTCGTCAACATGTTGTACGCTGATCTCAACTCTGAGAGGAGCATTACATGGGTGGATTGGTCAAGGGGTGGTCCTCATCCTGGGAAATTTGGATGGCCTGATATTACAGATGAGTTTTTGAATGGAATTCGGTTCGGATCGCAGTGCAAATACAACGGCAATACTACTTCATTATGTTTCTTATTTGGTAGGAAGTTTTTGCCTAATGCTCTGGAGCCTTTGTTACGAGTTGCTCCACTGCTGCTTGGCTTTGATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

429

Amino Acids

48.62

Weight (kDa)

7.2

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 160 - 386 4.9e-82 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 462
AasI GACNNNNNNGTC 1 cut(s) 552
Acc16I TGCGCA 1 cut(s) 579
AciI CCGC 7 cut(s) 200, 230, 269, 275, 281, 284, 898
AclWI GGATC 3 cut(s) 913, 1174, 1277
AcoI YGGCCR 1 cut(s) 936
AcsI RAATTY 3 cut(s) 533, 1115, 1155
AfaI GTAC 3 cut(s) 294, 396, 1046
AfiI CCNNNNNNNGG 4 cut(s) 654, 848, 934, 1109
AflIII ACRYGT 1 cut(s) 1038
AgsI TTSAA 7 cut(s) 62, 67, 161, 376, 920, 980, 1150
AhlI ACTAGT 1 cut(s) 362
AjnI CCWGG 5 cut(s) 117, 642, 841, 933, 1108
AluBI AGCT 2 cut(s) 388, 810
AluI AGCT 2 cut(s) 388, 810
Alw21I GWGCWC 1 cut(s) 927
Alw26I GTCTC 3 cut(s) 359, 924, 955
Alw44I GTGCAC 1 cut(s) 923
AlwI GGATC 3 cut(s) 913, 1174, 1277
Ama87I CYCGRG 1 cut(s) 832
AoxI GGCC 6 cut(s) 438, 503, 645, 872, 936, 1124
ApaLI GTGCAC 1 cut(s) 923
ApeKI GCWGC 6 cut(s) 8, 21, 24, 27, 601, 1270
ApoI RAATTY 3 cut(s) 533, 1115, 1155
ArsI GACNNNNNNTTYG 2 cut(s) 521, 553
AseI ATTAAT 3 cut(s) 180, 312, 636
AspLEI GCGC 2 cut(s) 580, 855
AspS9I GGNCC 4 cut(s) 439, 503, 873, 1100
AsuHPI GGTGA 1 cut(s) 204
AvaI CYCGRG 1 cut(s) 832
AvaII GGWCC 1 cut(s) 1100
BaeGI GKGCMC 1 cut(s) 927
BalI TGGCCA 1 cut(s) 938
BauI CACGAG 1 cut(s) 548
BbsI GAAGAC 1 cut(s) 610
Bbv12I GWGCWC 1 cut(s) 927
BbvI GCAGC 6 cut(s) 20, 33, 36, 39, 613, 1257
BccI CCATC 5 cut(s) 217, 226, 947, 980, 1116
BceAI ACGGC 3 cut(s) 632, 704, 1201
BcgI CGANNNNNNTGC 2 cut(s) 32, 66
BciT130I CCWGG 5 cut(s) 119, 644, 843, 935, 1110
BcoDI GTCTC 3 cut(s) 359, 924, 955
BcuI ACTAGT 1 cut(s) 362
BfaI CTAG 4 cut(s) 36, 363, 695, 1288
BfoI RGCGCY 1 cut(s) 856
BisI GCNGC 7 cut(s) 9, 22, 25, 28, 284, 602, 1271
BlsI GCNGC 7 cut(s) 10, 23, 26, 29, 285, 603, 1272
Bme1390I CCNGG 5 cut(s) 119, 644, 843, 935, 1110
Bme18I GGWCC 1 cut(s) 1100
BmeT110I CYCGRG 1 cut(s) 832
BmgT120I GGNCC 4 cut(s) 439, 503, 873, 1100
BmiI GGNNCC 4 cut(s) 441, 519, 875, 1245
BmrFI CCNGG 5 cut(s) 119, 644, 843, 935, 1110
BmrI ACTGGG 1 cut(s) 664
BmsI GCATC 4 cut(s) 123, 173, 440, 667
BmuI ACTGGG 1 cut(s) 664
BpiI GAAGAC 1 cut(s) 610
BpmI CTGGAG 1 cut(s) 1262
BpuEI CTTGAG 1 cut(s) 429
Bsa29I ATCGAT 1 cut(s) 1008
BsaI GGTCTC 1 cut(s) 924
BsaJI CCNNGG 4 cut(s) 435, 622, 833, 1109
BsaXI ACNNNNNCTCC 4 cut(s) 935, 965, 1235, 1265
Bsc4I CCNNNNNNNGG 4 cut(s) 654, 848, 934, 1109
Bse1I ACTGG 1 cut(s) 659
BseBI CCWGG 5 cut(s) 119, 644, 843, 935, 1110
BseCI ATCGAT 1 cut(s) 1008
BseDI CCNNGG 4 cut(s) 435, 622, 833, 1109
BseGI GGATG 6 cut(s) 114, 431, 682, 939, 1105, 1127
BseLI CCNNNNNNNGG 4 cut(s) 654, 848, 934, 1109
BseMII CTCAG 2 cut(s) 370, 1053
BseNI ACTGG 1 cut(s) 659
BseRI GAGGAG 3 cut(s) 246, 428, 1081
BseSI GKGCMC 1 cut(s) 927
BseXI GCAGC 6 cut(s) 20, 33, 36, 39, 613, 1257
BsgI GTGCAG 1 cut(s) 40
BshFI GGCC 6 cut(s) 440, 505, 647, 874, 938, 1126
BshVI ATCGAT 1 cut(s) 1008
BsiHKAI GWGCWC 1 cut(s) 927
BsiHKCI CYCGRG 1 cut(s) 832
BslFI GGGAC 2 cut(s) 542, 558
BslI CCNNNNNNNGG 4 cut(s) 654, 848, 934, 1109
BsmAI GTCTC 3 cut(s) 359, 924, 955
BsmFI GGGAC 2 cut(s) 542, 558
BsmI GAATGC 1 cut(s) 753
BsnI GGCC 6 cut(s) 440, 505, 647, 874, 938, 1126
Bso31I GGTCTC 1 cut(s) 924
BsoBI CYCGRG 1 cut(s) 832
Bsp1286I GDGCHC 1 cut(s) 927
Bsp143I GATC 5 cut(s) 905, 955, 1051, 1166, 1282
Bsp19I CCATGG 2 cut(s) 435, 622
BspACI CCGC 7 cut(s) 200, 230, 269, 275, 281, 284, 898
BspANI GGCC 6 cut(s) 440, 505, 647, 874, 938, 1126
BspCNI CTCAG 2 cut(s) 369, 1054
BspDI ATCGAT 1 cut(s) 1008
BspLI GGNNCC 4 cut(s) 441, 519, 875, 1245
BspPI GGATC 3 cut(s) 913, 1174, 1277
BspTNI GGTCTC 1 cut(s) 924
BsrI ACTGG 1 cut(s) 659
BssECI CCNNGG 4 cut(s) 435, 622, 833, 1109
BssMI GATC 5 cut(s) 905, 955, 1051, 1166, 1282
BssSI CACGAG 1 cut(s) 548
BssT1I CCWWGG 2 cut(s) 435, 622
Bst2BI CACGAG 1 cut(s) 548
Bst2UI CCWGG 5 cut(s) 119, 644, 843, 935, 1110
Bst4CI ACNGT 3 cut(s) 297, 347, 467
Bst6I CTCTTC 1 cut(s) 92
BstC8I GCNNGC 1 cut(s) 576
BstDEI CTNAG 3 cut(s) 356, 815, 1062
BstDSI CCRYGG 2 cut(s) 435, 622
BstENI CCTNNNNNAGG 1 cut(s) 846
BstF5I GGATG 6 cut(s) 114, 431, 682, 939, 1105, 1127
BstH2I RGCGCY 1 cut(s) 856
BstHHI GCGC 2 cut(s) 580, 855
BstKTI GATC 5 cut(s) 908, 958, 1054, 1169, 1285
BstMAI GTCTC 3 cut(s) 359, 924, 955
BstMBI GATC 5 cut(s) 905, 955, 1051, 1166, 1282
BstMWI GCNNNNNNNGC 5 cut(s) 27, 283, 357, 437, 816
BstNI CCWGG 5 cut(s) 119, 644, 843, 935, 1110
BstNSI RCATGY 2 cut(s) 578, 1042
BstSCI CCNGG 5 cut(s) 117, 642, 841, 933, 1108
BstSLI GKGCMC 1 cut(s) 927
BstV1I GCAGC 6 cut(s) 20, 33, 36, 39, 613, 1257
BstV2I GAAGAC 1 cut(s) 610
BstX2I RGATCY 1 cut(s) 905
BstYI RGATCY 1 cut(s) 905
Bsu15I ATCGAT 1 cut(s) 1008
BsuRI GGCC 6 cut(s) 440, 505, 647, 874, 938, 1126
BsuTUI ATCGAT 1 cut(s) 1008
BtgI CCRYGG 2 cut(s) 435, 622
BtsCI GGATG 6 cut(s) 114, 431, 682, 939, 1105, 1127
BtsI GCAGTG 4 cut(s) 37, 704, 1178, 1265
BtsIMutI CAGTG 5 cut(s) 37, 336, 704, 1178, 1265
Cac8I GCNNGC 1 cut(s) 576
CfoI GCGC 2 cut(s) 580, 855
Cfr13I GGNCC 4 cut(s) 439, 503, 873, 1100
ClaI ATCGAT 1 cut(s) 1008
Csp6I GTAC 3 cut(s) 293, 395, 1045
CspCI CAANNNNNGTGG 2 cut(s) 980, 1015
CviAII CATG 7 cut(s) 403, 436, 487, 575, 623, 1039, 1077
CviQI GTAC 3 cut(s) 293, 395, 1045
DdeI CTNAG 3 cut(s) 356, 815, 1062
DpnI GATC 5 cut(s) 907, 957, 1053, 1168, 1284
DpnII GATC 5 cut(s) 905, 955, 1051, 1166, 1282
DrdI GACNNNNNNGTC 1 cut(s) 552
DseDI GACNNNNNNGTC 1 cut(s) 552
EaeI YGGCCR 1 cut(s) 936
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
EciI GGCGGA 2 cut(s) 219, 913
Eco130I CCWWGG 2 cut(s) 435, 622
Eco31I GGTCTC 1 cut(s) 924
Eco47I GGWCC 1 cut(s) 1100
Eco88I CYCGRG 1 cut(s) 832
EcoNI CCTNNNNNAGG 1 cut(s) 846
EcoO109I RGGNCCY 1 cut(s) 503
EcoRI GAATTC 1 cut(s) 1155
EcoRII CCWGG 5 cut(s) 117, 642, 841, 933, 1108
EcoT14I CCWWGG 2 cut(s) 435, 622
ErhI CCWWGG 2 cut(s) 435, 622
FaeI CATG 7 cut(s) 406, 439, 490, 578, 626, 1042, 1080
FaqI GGGAC 2 cut(s) 542, 558
FatI CATG 7 cut(s) 402, 435, 486, 574, 622, 1038, 1076
Fnu4HI GCNGC 7 cut(s) 9, 22, 25, 28, 284, 602, 1271
FokI GGATG 6 cut(s) 101, 418, 689, 926, 1092, 1134
Fsp4HI GCNGC 7 cut(s) 9, 22, 25, 28, 284, 602, 1271
FspAI RTGCGCAY 1 cut(s) 579
FspBI CTAG 4 cut(s) 36, 363, 695, 1288
FspI TGCGCA 1 cut(s) 579
GlaI GCGC 2 cut(s) 579, 854
GluI GCNGC 7 cut(s) 9, 22, 25, 28, 284, 602, 1271
GsuI CTGGAG 1 cut(s) 1262
HaeII RGCGCY 1 cut(s) 856
HaeIII GGCC 6 cut(s) 440, 505, 647, 874, 938, 1126
HhaI GCGC 2 cut(s) 580, 855
Hin1II CATG 7 cut(s) 406, 439, 490, 578, 626, 1042, 1080
Hin6I GCGC 2 cut(s) 578, 853
HinP1I GCGC 2 cut(s) 578, 853
HincII GTYRAC 1 cut(s) 1036
HindII GTYRAC 1 cut(s) 1036
HinfI GANTC 3 cut(s) 452, 596, 746
HphI GGTGA 1 cut(s) 204
Hpy166II GTNNAC 5 cut(s) 397, 652, 796, 925, 1036
Hpy188I TCNGA 6 cut(s) 474, 745, 955, 960, 1063, 1166
Hpy188III TCNNGA 4 cut(s) 136, 493, 772, 1241
Hpy8I GTNNAC 5 cut(s) 397, 652, 796, 925, 1036
Hpy99I CGWCG 2 cut(s) 413, 690
HpyAV CCTTC 1 cut(s) 594
HpyCH4III ACNGT 3 cut(s) 297, 347, 467
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 9 cut(s) 21, 79, 317, 574, 680, 704, 753, 925, 1176
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 283, 357, 437, 816
HpyF3I CTNAG 3 cut(s) 356, 815, 1062
HpySE526I ACGT 1 cut(s) 72
Hsp92II CATG 7 cut(s) 406, 439, 490, 578, 626, 1042, 1080
HspAI GCGC 2 cut(s) 578, 853
Kzo9I GATC 5 cut(s) 905, 955, 1051, 1166, 1282
LmnI GCTCC 4 cut(s) 523, 1068, 1243, 1267
Lsp1109I GCAGC 6 cut(s) 20, 33, 36, 39, 613, 1257
LweI GCATC 4 cut(s) 123, 173, 440, 667
MaeI CTAG 4 cut(s) 36, 363, 695, 1288
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 5 cut(s) 105, 140, 151, 417, 1251
MalI GATC 5 cut(s) 907, 957, 1053, 1168, 1284
MboI GATC 5 cut(s) 905, 955, 1051, 1166, 1282
MboII GAAGA 3 cut(s) 79, 610, 642
MflI RGATCY 1 cut(s) 905
MhlI GDGCHC 1 cut(s) 927
MlsI TGGCCA 1 cut(s) 938
MluNI TGGCCA 1 cut(s) 938
MmeI TCCRAC 2 cut(s) 654, 1049
Mox20I TGGCCA 1 cut(s) 938
MscI TGGCCA 1 cut(s) 938
MseI TTAA 7 cut(s) 180, 192, 312, 339, 591, 636, 765
Msp20I TGGCCA 1 cut(s) 938
MspR9I CCNGG 5 cut(s) 119, 644, 843, 935, 1110
Mva1269I GAATGC 1 cut(s) 753
MvaI CCWGG 5 cut(s) 119, 644, 843, 935, 1110
MwoI GCNNNNNNNGC 5 cut(s) 27, 283, 357, 437, 816
NcoI CCATGG 2 cut(s) 435, 622
NdeII GATC 5 cut(s) 905, 955, 1051, 1166, 1282
NlaIII CATG 7 cut(s) 406, 439, 490, 578, 626, 1042, 1080
NlaIV GGNNCC 4 cut(s) 441, 519, 875, 1245
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 2 cut(s) 105, 151
NsbI TGCGCA 1 cut(s) 579
NspI RCATGY 2 cut(s) 578, 1042
PaeI GCATGC 1 cut(s) 578
PciI ACATGT 1 cut(s) 1038
PctI GAATGC 1 cut(s) 753
PfeI GAWTC 3 cut(s) 452, 596, 746
PkrI GCNGC 7 cut(s) 10, 23, 26, 29, 285, 603, 1272
PscI ACATGT 1 cut(s) 1038
PshBI ATTAAT 3 cut(s) 180, 312, 636
PsiI TTATAA 1 cut(s) 462
Psp6I CCWGG 5 cut(s) 117, 642, 841, 933, 1108
PspGI CCWGG 5 cut(s) 117, 642, 841, 933, 1108
PspN4I GGNNCC 4 cut(s) 441, 519, 875, 1245
PspPI GGNCC 4 cut(s) 439, 503, 873, 1100
PsuI RGATCY 1 cut(s) 905
RsaI GTAC 3 cut(s) 294, 396, 1046
RsaNI GTAC 3 cut(s) 293, 395, 1045
SaqAI TTAA 7 cut(s) 180, 192, 312, 339, 591, 636, 765
SatI GCNGC 7 cut(s) 9, 22, 25, 28, 284, 602, 1271
Sau3AI GATC 5 cut(s) 905, 955, 1051, 1166, 1282
Sau96I GGNCC 4 cut(s) 439, 503, 873, 1100
ScrFI CCNGG 5 cut(s) 119, 644, 843, 935, 1110
SduI GDGCHC 1 cut(s) 927
SfaNI GCATC 4 cut(s) 123, 173, 440, 667
SinI GGWCC 1 cut(s) 1100
SmlI CTYRAG 1 cut(s) 444
SmoI CTYRAG 1 cut(s) 444
SpeI ACTAGT 1 cut(s) 362
SphI GCATGC 1 cut(s) 578
SsiI CCGC 7 cut(s) 200, 230, 269, 275, 281, 284, 898
SspMI CTAG 4 cut(s) 36, 363, 695, 1288
StyD4I CCNGG 5 cut(s) 117, 642, 841, 933, 1108
StyI CCWWGG 2 cut(s) 435, 622
TaaI ACNGT 3 cut(s) 297, 347, 467
TaiI ACGT 1 cut(s) 75
TaqI TCGA 4 cut(s) 628, 825, 847, 1008
TatI WGTACW 1 cut(s) 394
TauI GCSGC 1 cut(s) 286
TfiI GAWTC 3 cut(s) 452, 596, 746
Tru1I TTAA 7 cut(s) 180, 192, 312, 339, 591, 636, 765
Tru9I TTAA 7 cut(s) 180, 192, 312, 339, 591, 636, 765
TscAI CASTG 5 cut(s) 37, 336, 711, 1178, 1272
TseFI GTSAC 2 cut(s) 105, 151
TseI GCWGC 6 cut(s) 8, 21, 24, 27, 601, 1270
Tsp45I GTSAC 2 cut(s) 105, 151
TspDTI ATGAA 3 cut(s) 475, 609, 1188
TspRI CASTG 5 cut(s) 37, 336, 711, 1178, 1272
VneI GTGCAC 1 cut(s) 923
VpaK11BI GGWCC 1 cut(s) 1100
VspI ATTAAT 3 cut(s) 180, 312, 636
XagI CCTNNNNNAGG 1 cut(s) 846
XapI RAATTY 3 cut(s) 533, 1115, 1155
XceI RCATGY 2 cut(s) 578, 1042
XspI CTAG 4 cut(s) 36, 363, 695, 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.