ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| LON_substr_bdg | PF02190 | 145 - 352 | 1.2e-36 | ATP-dependent protease La (LON) substrate-binding domain |
| AAA | PF00004 | 505 - 642 | 3.9e-24 | ATPase family associated with various cellular activities (AAA) |
| Lon_lid | PF22667 | 668 - 717 | 2.3e-19 | Lon protease AAA+ ATPase lid domain |
| Lon_C | PF05362 | 774 - 978 | 2.3e-79 | Lon protease (S16) C-terminal proteolytic domain |
| ChlI | PF13541 | 826 - 947 | 1.6e-06 | Subunit ChlI of Mg-chelatase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 86 |
| AarI | CACCTGC | 1 cut(s) | 2069 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 818 |
| Acc36I | ACCTGC | 1 cut(s) | 2069 |
| AccB1I | GGYRCC | 2 cut(s) | 448, 1658 |
| AccBSI | CCGCTC | 1 cut(s) | 959 |
| AccI | GTMKAC | 1 cut(s) | 28 |
| AccII | CGCG | 1 cut(s) | 1333 |
| AclWI | GGATC | 6 cut(s) | 494, 507, 1434, 1787, 1800, 2177 |
| AcoI | YGGCCR | 1 cut(s) | 1767 |
| AcsI | RAATTY | 6 cut(s) | 47, 1245, 1466, 1586, 1810, 2757 |
| AcuI | CTGAAG | 7 cut(s) | 351, 384, 438, 590, 890, 2208, 2286 |
| AcvI | CACGTG | 2 cut(s) | 1571, 1994 |
| AcyI | GRCGYC | 1 cut(s) | 1659 |
| AdeI | CACNNNGTG | 2 cut(s) | 255, 2899 |
| AfaI | GTAC | 3 cut(s) | 510, 867, 2447 |
| AfeI | AGCGCT | 1 cut(s) | 2043 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 314, 554, 571, 1538, 2648, 2660, 2771 |
| AflII | CTTAAG | 1 cut(s) | 788 |
| AflIII | ACRYGT | 1 cut(s) | 1331 |
| AgeI | ACCGGT | 2 cut(s) | 2356, 2392 |
| AhlI | ACTAGT | 1 cut(s) | 1547 |
| AjnI | CCWGG | 3 cut(s) | 469, 1531, 1666 |
| Alw21I | GWGCWC | 3 cut(s) | 1576, 1871, 2536 |
| Alw26I | GTCTC | 6 cut(s) | 225, 696, 1175, 1965, 2447, 2841 |
| Alw44I | GTGCAC | 2 cut(s) | 1867, 2532 |
| AlwI | GGATC | 6 cut(s) | 494, 507, 1434, 1787, 1800, 2177 |
| Ama87I | CYCGRG | 1 cut(s) | 165 |
| Aor51HI | AGCGCT | 1 cut(s) | 2043 |
| AoxI | GGCC | 7 cut(s) | 413, 458, 1525, 1750, 1767, 2283, 2488 |
| ApaLI | GTGCAC | 2 cut(s) | 1867, 2532 |
| ApeKI | GCWGC | 6 cut(s) | 515, 1147, 1291, 1294, 2039, 2664 |
| ApoI | RAATTY | 6 cut(s) | 47, 1245, 1466, 1586, 1810, 2757 |
| AsiGI | ACCGGT | 2 cut(s) | 2356, 2392 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 1245, 1956, 2591 |
| AspA2I | CCTAGG | 1 cut(s) | 410 |
| AspLEI | GCGC | 5 cut(s) | 974, 1255, 1408, 1661, 2044 |
| AspS9I | GGNCC | 5 cut(s) | 1526, 1819, 2284, 2651, 2719 |
| AsuC2I | CCSGG | 1 cut(s) | 1766 |
| AsuHPI | GGTGA | 8 cut(s) | 763, 935, 1130, 1772, 1933, 2041, 2525, 2788 |
| AvaI | CYCGRG | 1 cut(s) | 165 |
| AvaII | GGWCC | 3 cut(s) | 1819, 2651, 2719 |
| AvrII | CCTAGG | 1 cut(s) | 410 |
| BaeGI | GKGCMC | 3 cut(s) | 402, 1871, 2536 |
| BamHI | GGATCC | 2 cut(s) | 499, 1792 |
| BanI | GGYRCC | 2 cut(s) | 448, 1658 |
| BbrPI | CACGTG | 2 cut(s) | 1571, 1994 |
| BbsI | GAAGAC | 4 cut(s) | 198, 541, 878, 1489 |
| Bbv12I | GWGCWC | 3 cut(s) | 1576, 1871, 2536 |
| BbvI | GCAGC | 6 cut(s) | 527, 1159, 1278, 1306, 2051, 2651 |
| BccI | CCATC | 5 cut(s) | 589, 742, 1669, 2401, 2642 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2150, 2184 |
| BciT130I | CCWGG | 3 cut(s) | 471, 1533, 1668 |
| BciVI | GTATCC | 1 cut(s) | 704 |
| BclI | TGATCA | 1 cut(s) | 2673 |
| BcnI | CCSGG | 1 cut(s) | 1766 |
| BcoDI | GTCTC | 6 cut(s) | 225, 696, 1175, 1965, 2447, 2841 |
| BcuI | ACTAGT | 1 cut(s) | 1547 |
| BfaI | CTAG | 7 cut(s) | 411, 437, 944, 1548, 1907, 2414, 2570 |
| BfmI | CTRYAG | 7 cut(s) | 29, 1292, 1839, 2068, 2169, 2628, 2790 |
| BfoI | RGCGCY | 2 cut(s) | 1662, 2045 |
| BfrI | CTTAAG | 1 cut(s) | 788 |
| BfuAI | ACCTGC | 1 cut(s) | 2069 |
| BfuI | GTATCC | 1 cut(s) | 704 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 73, 457, 1524 |
| BglII | AGATCT | 3 cut(s) | 116, 1843, 2916 |
| BlnI | CCTAGG | 1 cut(s) | 410 |
| BmcAI | AGTACT | 1 cut(s) | 510 |
| Bme1390I | CCNGG | 4 cut(s) | 471, 1533, 1668, 1766 |
| Bme18I | GGWCC | 3 cut(s) | 1819, 2651, 2719 |
| BmeT110I | CYCGRG | 1 cut(s) | 165 |
| BmgT120I | GGNCC | 5 cut(s) | 1526, 1819, 2284, 2651, 2719 |
| BmiI | GGNNCC | 5 cut(s) | 450, 501, 1660, 1794, 2635 |
| BmrFI | CCNGG | 4 cut(s) | 471, 1533, 1668, 1766 |
| BmrI | ACTGGG | 1 cut(s) | 261 |
| BmsI | GCATC | 3 cut(s) | 991, 1648, 2026 |
| BmuI | ACTGGG | 1 cut(s) | 261 |
| BpiI | GAAGAC | 4 cut(s) | 198, 541, 878, 1489 |
| BpmI | CTGGAG | 5 cut(s) | 27, 195, 1554, 2078, 2754 |
| BpuEI | CTTGAG | 2 cut(s) | 975, 1136 |
| BpuMI | CCSGG | 1 cut(s) | 1766 |
| Bsa29I | ATCGAT | 1 cut(s) | 1725 |
| BsaAI | YACGTR | 2 cut(s) | 1571, 1994 |
| BsaBI | GATNNNNATC | 1 cut(s) | 18 |
| BsaHI | GRCGYC | 1 cut(s) | 1659 |
| BsaI | GGTCTC | 1 cut(s) | 696 |
| BsaJI | CCNNGG | 4 cut(s) | 410, 697, 1364, 2643 |
| BsaWI | WCCGGW | 6 cut(s) | 184, 1593, 2356, 2392, 2750, 2841 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 693, 723 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 314, 554, 571, 1538, 2648, 2660, 2771 |
| Bse118I | RCCGGY | 4 cut(s) | 445, 654, 2356, 2392 |
| Bse1I | ACTGG | 6 cut(s) | 212, 256, 1014, 1467, 1770, 2737 |
| Bse3DI | GCAATG | 3 cut(s) | 440, 1688, 2733 |
| Bse8I | GATNNNNATC | 1 cut(s) | 18 |
| BseBI | CCWGG | 3 cut(s) | 471, 1533, 1668 |
| BseCI | ATCGAT | 1 cut(s) | 1725 |
| BseDI | CCNNGG | 4 cut(s) | 410, 697, 1364, 2643 |
| BseGI | GGATG | 5 cut(s) | 580, 868, 1018, 2653, 2908 |
| BseJI | GATNNNNATC | 1 cut(s) | 18 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 314, 554, 571, 1538, 2648, 2660, 2771 |
| BseMI | GCAATG | 3 cut(s) | 440, 1688, 2733 |
| BseMII | CTCAG | 3 cut(s) | 294, 695, 1617 |
| BseNI | ACTGG | 6 cut(s) | 212, 256, 1014, 1467, 1770, 2737 |
| BseSI | GKGCMC | 3 cut(s) | 402, 1871, 2536 |
| BseXI | GCAGC | 6 cut(s) | 527, 1159, 1278, 1306, 2051, 2651 |
| BseYI | CCCAGC | 3 cut(s) | 912, 1741, 1934 |
| BsgI | GTGCAG | 4 cut(s) | 1182, 1974, 2650, 2679 |
| Bsh1236I | CGCG | 1 cut(s) | 1333 |
| BshFI | GGCC | 7 cut(s) | 415, 460, 1527, 1752, 1769, 2285, 2490 |
| BshNI | GGYRCC | 2 cut(s) | 448, 1658 |
| BshTI | ACCGGT | 2 cut(s) | 2356, 2392 |
| BshVI | ATCGAT | 1 cut(s) | 1725 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1576, 1871, 2536 |
| BsiHKCI | CYCGRG | 1 cut(s) | 165 |
| BslFI | GGGAC | 2 cut(s) | 1714, 2141 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 314, 554, 571, 1538, 2648, 2660, 2771 |
| BsmAI | GTCTC | 6 cut(s) | 225, 696, 1175, 1965, 2447, 2841 |
| BsmBI | CGTCTC | 1 cut(s) | 2447 |
| BsmFI | GGGAC | 2 cut(s) | 1714, 2141 |
| BsnI | GGCC | 7 cut(s) | 415, 460, 1527, 1752, 1769, 2285, 2490 |
| Bso31I | GGTCTC | 1 cut(s) | 696 |
| BsoBI | CYCGRG | 1 cut(s) | 165 |
| Bsp1286I | GDGCHC | 4 cut(s) | 402, 1576, 1871, 2536 |
| Bsp1407I | TGTACA | 1 cut(s) | 2445 |
| BspANI | GGCC | 7 cut(s) | 415, 460, 1527, 1752, 1769, 2285, 2490 |
| BspCNI | CTCAG | 3 cut(s) | 295, 694, 1618 |
| BspDI | ATCGAT | 1 cut(s) | 1725 |
| BspFNI | CGCG | 1 cut(s) | 1333 |
| BspLI | GGNNCC | 5 cut(s) | 450, 501, 1660, 1794, 2635 |
| BspMAI | CTGCAG | 4 cut(s) | 1296, 2072, 2173, 2632 |
| BspMI | ACCTGC | 1 cut(s) | 2069 |
| BspPI | GGATC | 6 cut(s) | 494, 507, 1434, 1787, 1800, 2177 |
| BspQI | GCTCTTC | 3 cut(s) | 152, 1272, 2157 |
| BspT107I | GGYRCC | 2 cut(s) | 448, 1658 |
| BspTI | CTTAAG | 1 cut(s) | 788 |
| BspTNI | GGTCTC | 1 cut(s) | 696 |
| BsrBI | CCGCTC | 1 cut(s) | 959 |
| BsrDI | GCAATG | 3 cut(s) | 440, 1688, 2733 |
| BsrFI | RCCGGY | 4 cut(s) | 445, 654, 2356, 2392 |
| BsrGI | TGTACA | 1 cut(s) | 2445 |
| BsrI | ACTGG | 6 cut(s) | 212, 256, 1014, 1467, 1770, 2737 |
| BssAI | RCCGGY | 4 cut(s) | 445, 654, 2356, 2392 |
| BssECI | CCNNGG | 4 cut(s) | 410, 697, 1364, 2643 |
| BssNI | GRCGYC | 1 cut(s) | 1659 |
| BssT1I | CCWWGG | 4 cut(s) | 410, 697, 1364, 2643 |
| Bst2UI | CCWGG | 3 cut(s) | 471, 1533, 1668 |
| Bst4CI | ACNGT | 9 cut(s) | 433, 772, 1379, 2120, 2239, 2254, 2314, 2461, 2551 |
| Bst6I | CTCTTC | 4 cut(s) | 152, 1272, 2157, 2274 |
| BstACI | GRCGYC | 1 cut(s) | 1659 |
| BstAFI | CTTAAG | 1 cut(s) | 788 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1205, 2540 |
| BstAUI | TGTACA | 1 cut(s) | 2445 |
| BstBAI | YACGTR | 2 cut(s) | 1571, 1994 |
| BstC8I | GCNNGC | 4 cut(s) | 2169, 2662, 2862, 2928 |
| BstDEI | CTNAG | 6 cut(s) | 303, 342, 681, 857, 1487, 1626 |
| BstEII | GGTNACC | 1 cut(s) | 1760 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 569, 1536 |
| BstF5I | GGATG | 5 cut(s) | 580, 868, 1018, 2653, 2908 |
| BstFNI | CGCG | 1 cut(s) | 1333 |
| BstH2I | RGCGCY | 2 cut(s) | 1662, 2045 |
| BstHHI | GCGC | 5 cut(s) | 974, 1255, 1408, 1661, 2044 |
| BstMAI | GTCTC | 6 cut(s) | 225, 696, 1175, 1965, 2447, 2841 |
| BstNI | CCWGG | 3 cut(s) | 471, 1533, 1668 |
| BstNSI | RCATGY | 2 cut(s) | 484, 1259 |
| BstPI | GGTNACC | 1 cut(s) | 1760 |
| BstSCI | CCNGG | 4 cut(s) | 469, 1531, 1666, 1764 |
| BstSFI | CTRYAG | 7 cut(s) | 29, 1292, 1839, 2068, 2169, 2628, 2790 |
| BstSLI | GKGCMC | 3 cut(s) | 402, 1871, 2536 |
| BstUI | CGCG | 1 cut(s) | 1333 |
| BstV1I | GCAGC | 6 cut(s) | 527, 1159, 1278, 1306, 2051, 2651 |
| BstV2I | GAAGAC | 4 cut(s) | 198, 541, 878, 1489 |
| BstX2I | RGATCY | 5 cut(s) | 116, 499, 1792, 1843, 2916 |
| BstYI | RGATCY | 5 cut(s) | 116, 499, 1792, 1843, 2916 |
| Bsu15I | ATCGAT | 1 cut(s) | 1725 |
| BsuI | GTATCC | 1 cut(s) | 704 |
| BsuRI | GGCC | 7 cut(s) | 415, 460, 1527, 1752, 1769, 2285, 2490 |
| BsuTUI | ATCGAT | 1 cut(s) | 1725 |
| BtsCI | GGATG | 5 cut(s) | 580, 868, 1018, 2653, 2908 |
| BtsI | GCAGTG | 1 cut(s) | 1482 |
| BtsIMutI | CAGTG | 7 cut(s) | 219, 249, 603, 1482, 1777, 1944, 2250 |
| BveI | ACCTGC | 1 cut(s) | 2069 |
| Cac8I | GCNNGC | 4 cut(s) | 2169, 2662, 2862, 2928 |
| CfoI | GCGC | 5 cut(s) | 974, 1255, 1408, 1661, 2044 |
| Cfr10I | RCCGGY | 4 cut(s) | 445, 654, 2356, 2392 |
| Cfr13I | GGNCC | 5 cut(s) | 1526, 1819, 2284, 2651, 2719 |
| ClaI | ATCGAT | 1 cut(s) | 1725 |
| CseI | GACGC | 3 cut(s) | 617, 1045, 1339 |
| Csp6I | GTAC | 3 cut(s) | 509, 866, 2446 |
| CspAI | ACCGGT | 2 cut(s) | 2356, 2392 |
| CviQI | GTAC | 3 cut(s) | 509, 866, 2446 |
| DdeI | CTNAG | 6 cut(s) | 303, 342, 681, 857, 1487, 1626 |
| DinI | GGCGCC | 1 cut(s) | 1660 |
| DraIII | CACNNNGTG | 2 cut(s) | 255, 2899 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 818 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 818 |
| EaeI | YGGCCR | 1 cut(s) | 1767 |
| Eam1104I | CTCTTC | 4 cut(s) | 152, 1272, 2157, 2274 |
| EarI | CTCTTC | 4 cut(s) | 152, 1272, 2157, 2274 |
| EciI | GGCGGA | 2 cut(s) | 148, 2818 |
| Eco130I | CCWWGG | 4 cut(s) | 410, 697, 1364, 2643 |
| Eco147I | AGGCCT | 1 cut(s) | 415 |
| Eco31I | GGTCTC | 1 cut(s) | 696 |
| Eco47I | GGWCC | 3 cut(s) | 1819, 2651, 2719 |
| Eco47III | AGCGCT | 1 cut(s) | 2043 |
| Eco57I | CTGAAG | 7 cut(s) | 351, 384, 438, 590, 890, 2208, 2286 |
| Eco72I | CACGTG | 2 cut(s) | 1571, 1994 |
| Eco88I | CYCGRG | 1 cut(s) | 165 |
| Eco91I | GGTNACC | 1 cut(s) | 1760 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 569, 1536 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2719 |
| EcoO65I | GGTNACC | 1 cut(s) | 1760 |
| EcoRI | GAATTC | 2 cut(s) | 47, 2757 |
| EcoRII | CCWGG | 3 cut(s) | 469, 1531, 1666 |
| EcoT14I | CCWWGG | 4 cut(s) | 410, 697, 1364, 2643 |
| EgeI | GGCGCC | 1 cut(s) | 1660 |
| EheI | GGCGCC | 1 cut(s) | 1660 |
| ErhI | CCWWGG | 4 cut(s) | 410, 697, 1364, 2643 |
| Esp3I | CGTCTC | 1 cut(s) | 2447 |
| FaqI | GGGAC | 2 cut(s) | 1714, 2141 |
| FauNDI | CATATG | 2 cut(s) | 550, 1651 |
| FbaI | TGATCA | 1 cut(s) | 2673 |
| FblI | GTMKAC | 1 cut(s) | 28 |
| FokI | GGATG | 5 cut(s) | 587, 875, 1025, 2660, 2915 |
| FspBI | CTAG | 7 cut(s) | 411, 437, 944, 1548, 1907, 2414, 2570 |
| GlaI | GCGC | 5 cut(s) | 973, 1254, 1407, 1660, 2043 |
| GsaI | CCCAGC | 3 cut(s) | 916, 1745, 1938 |
| GsuI | CTGGAG | 5 cut(s) | 27, 195, 1554, 2078, 2754 |
| HaeII | RGCGCY | 2 cut(s) | 1662, 2045 |
| HaeIII | GGCC | 7 cut(s) | 415, 460, 1527, 1752, 1769, 2285, 2490 |
| HgaI | GACGC | 3 cut(s) | 617, 1045, 1339 |
| HhaI | GCGC | 5 cut(s) | 974, 1255, 1408, 1661, 2044 |
| Hin1I | GRCGYC | 1 cut(s) | 1659 |
| Hin6I | GCGC | 5 cut(s) | 972, 1253, 1406, 1659, 2042 |
| HinP1I | GCGC | 5 cut(s) | 972, 1253, 1406, 1659, 2042 |
| HincII | GTYRAC | 2 cut(s) | 1035, 1353 |
| HindII | GTYRAC | 2 cut(s) | 1035, 1353 |
| HindIII | AAGCTT | 1 cut(s) | 2609 |
| HphI | GGTGA | 8 cut(s) | 763, 935, 1130, 1772, 1933, 2041, 2525, 2788 |
| Hpy166II | GTNNAC | 9 cut(s) | 29, 172, 881, 1018, 1035, 1353, 1869, 2534, 2893 |
| Hpy8I | GTNNAC | 9 cut(s) | 29, 172, 881, 1018, 1035, 1353, 1869, 2534, 2893 |
| Hpy99I | CGWCG | 4 cut(s) | 203, 1131, 1647, 1835 |
| HpyAV | CCTTC | 8 cut(s) | 565, 575, 1159, 2141, 2465, 2471, 2710, 2873 |
| HpyCH4III | ACNGT | 9 cut(s) | 433, 772, 1379, 2120, 2239, 2254, 2314, 2461, 2551 |
| HpyCH4IV | ACGT | 8 cut(s) | 897, 1024, 1126, 1326, 1446, 1570, 1833, 1993 |
| HpyF3I | CTNAG | 6 cut(s) | 303, 342, 681, 857, 1487, 1626 |
| HpySE526I | ACGT | 8 cut(s) | 897, 1024, 1126, 1326, 1446, 1570, 1833, 1993 |
| Hsp92I | GRCGYC | 1 cut(s) | 1659 |
| HspAI | GCGC | 5 cut(s) | 972, 1253, 1406, 1659, 2042 |
| KasI | GGCGCC | 1 cut(s) | 1658 |
| Ksp22I | TGATCA | 1 cut(s) | 2673 |
| LguI | GCTCTTC | 3 cut(s) | 152, 1272, 2157 |
| LmnI | GCTCC | 7 cut(s) | 550, 557, 956, 1168, 1783, 2150, 2633 |
| Lsp1109I | GCAGC | 6 cut(s) | 527, 1159, 1278, 1306, 2051, 2651 |
| LweI | GCATC | 3 cut(s) | 991, 1648, 2026 |
| MaeI | CTAG | 7 cut(s) | 411, 437, 944, 1548, 1907, 2414, 2570 |
| MaeII | ACGT | 8 cut(s) | 897, 1024, 1126, 1326, 1446, 1570, 1833, 1993 |
| MaeIII | GTNAC | 8 cut(s) | 107, 722, 1130, 1327, 1334, 1760, 1834, 2740 |
| MbiI | CCGCTC | 1 cut(s) | 959 |
| MfeI | CAATTG | 1 cut(s) | 1098 |
| MflI | RGATCY | 5 cut(s) | 116, 499, 1792, 1843, 2916 |
| MhlI | GDGCHC | 4 cut(s) | 402, 1576, 1871, 2536 |
| MluI | ACGCGT | 1 cut(s) | 1331 |
| Mly113I | GGCGCC | 1 cut(s) | 1659 |
| MlyI | GAGTC | 8 cut(s) | 175, 177, 227, 250, 362, 368, 2336, 2815 |
| MmeI | TCCRAC | 3 cut(s) | 1585, 1625, 1761 |
| MroXI | GAANNNNTTC | 3 cut(s) | 1245, 1956, 2591 |
| MslI | CAYNNNNRTG | 3 cut(s) | 921, 1758, 2619 |
| MspA1I | CMGCKG | 2 cut(s) | 587, 1297 |
| MspCI | CTTAAG | 1 cut(s) | 788 |
| MspR9I | CCNGG | 4 cut(s) | 471, 1533, 1668, 1766 |
| MunI | CAATTG | 1 cut(s) | 1098 |
| MvaI | CCWGG | 3 cut(s) | 471, 1533, 1668 |
| MvnI | CGCG | 1 cut(s) | 1333 |
| NarI | GGCGCC | 1 cut(s) | 1659 |
| NciI | CCSGG | 1 cut(s) | 1766 |
| NdeI | CATATG | 2 cut(s) | 550, 1651 |
| NlaIV | GGNNCC | 5 cut(s) | 450, 501, 1660, 1794, 2635 |
| NmuCI | GTSAC | 4 cut(s) | 722, 1327, 1760, 2740 |
| NspI | RCATGY | 2 cut(s) | 484, 1259 |
| PaeR7I | CTCGAG | 1 cut(s) | 165 |
| PaqCI | CACCTGC | 1 cut(s) | 2069 |
| PceI | AGGCCT | 1 cut(s) | 415 |
| PciSI | GCTCTTC | 3 cut(s) | 152, 1272, 2157 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 819 |
| PdmI | GAANNNNTTC | 3 cut(s) | 1245, 1956, 2591 |
| PfeI | GAWTC | 8 cut(s) | 259, 359, 590, 1891, 2089, 2219, 2230, 2587 |
| PfoI | TCCNGGA | 1 cut(s) | 1531 |
| PinAI | ACCGGT | 2 cut(s) | 2356, 2392 |
| PleI | GAGTC | 8 cut(s) | 175, 176, 226, 249, 361, 368, 2335, 2814 |
| PluTI | GGCGCC | 1 cut(s) | 1662 |
| PmaCI | CACGTG | 2 cut(s) | 1571, 1994 |
| PmlI | CACGTG | 2 cut(s) | 1571, 1994 |
| PpsI | GAGTC | 8 cut(s) | 175, 176, 226, 249, 361, 368, 2335, 2814 |
| Ppu21I | YACGTR | 2 cut(s) | 1571, 1994 |
| PpuMI | RGGWCCY | 1 cut(s) | 2719 |
| PsiI | TTATAA | 1 cut(s) | 86 |
| Psp5II | RGGWCCY | 1 cut(s) | 2719 |
| Psp6I | CCWGG | 3 cut(s) | 469, 1531, 1666 |
| PspCI | CACGTG | 2 cut(s) | 1571, 1994 |
| PspEI | GGTNACC | 1 cut(s) | 1760 |
| PspFI | CCCAGC | 3 cut(s) | 912, 1741, 1934 |
| PspGI | CCWGG | 3 cut(s) | 469, 1531, 1666 |
| PspN4I | GGNNCC | 5 cut(s) | 450, 501, 1660, 1794, 2635 |
| PspPI | GGNCC | 5 cut(s) | 1526, 1819, 2284, 2651, 2719 |
| PspPPI | RGGWCCY | 1 cut(s) | 2719 |
| PstI | CTGCAG | 4 cut(s) | 1296, 2072, 2173, 2632 |
| PsuI | RGATCY | 5 cut(s) | 116, 499, 1792, 1843, 2916 |
| PvuII | CAGCTG | 2 cut(s) | 587, 1297 |
| RsaI | GTAC | 3 cut(s) | 510, 867, 2447 |
| RsaNI | GTAC | 3 cut(s) | 509, 866, 2446 |
| RseI | CAYNNNNRTG | 3 cut(s) | 921, 1758, 2619 |
| SapI | GCTCTTC | 3 cut(s) | 152, 1272, 2157 |
| Sau96I | GGNCC | 5 cut(s) | 1526, 1819, 2284, 2651, 2719 |
| SbfI | CCTGCAGG | 1 cut(s) | 2632 |
| ScaI | AGTACT | 1 cut(s) | 510 |
| SchI | GAGTC | 8 cut(s) | 175, 177, 227, 250, 362, 368, 2336, 2815 |
| ScrFI | CCNGG | 4 cut(s) | 471, 1533, 1668, 1766 |
| SdaI | CCTGCAGG | 1 cut(s) | 2632 |
| SduI | GDGCHC | 4 cut(s) | 402, 1576, 1871, 2536 |
| SfaNI | GCATC | 3 cut(s) | 991, 1648, 2026 |
| SfcI | CTRYAG | 7 cut(s) | 29, 1292, 1839, 2068, 2169, 2628, 2790 |
| SfoI | GGCGCC | 1 cut(s) | 1660 |
| Sfr274I | CTCGAG | 1 cut(s) | 165 |
| SinI | GGWCC | 3 cut(s) | 1819, 2651, 2719 |
| SlaI | CTCGAG | 1 cut(s) | 165 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 921, 1758, 2619 |
| SmlI | CTYRAG | 4 cut(s) | 165, 788, 990, 1151 |
| SmoI | CTYRAG | 4 cut(s) | 165, 788, 990, 1151 |
| SpeI | ACTAGT | 1 cut(s) | 1547 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 2632 |
| SseBI | AGGCCT | 1 cut(s) | 415 |
| SspDI | GGCGCC | 1 cut(s) | 1658 |
| SspI | AATATT | 1 cut(s) | 2826 |
| SspMI | CTAG | 7 cut(s) | 411, 437, 944, 1548, 1907, 2414, 2570 |
| StuI | AGGCCT | 1 cut(s) | 415 |
| StyD4I | CCNGG | 4 cut(s) | 469, 1531, 1666, 1764 |
| StyI | CCWWGG | 4 cut(s) | 410, 697, 1364, 2643 |
| TaaI | ACNGT | 9 cut(s) | 433, 772, 1379, 2120, 2239, 2254, 2314, 2461, 2551 |
| TaiI | ACGT | 8 cut(s) | 900, 1027, 1129, 1329, 1449, 1573, 1836, 1996 |
| TaqI | TCGA | 7 cut(s) | 166, 780, 1725, 1830, 2106, 2241, 2922 |
| TatI | WGTACW | 3 cut(s) | 508, 865, 2445 |
| TauI | GCSGC | 5 cut(s) | 326, 329, 463, 962, 2798 |
| TfiI | GAWTC | 8 cut(s) | 259, 359, 590, 1891, 2089, 2219, 2230, 2587 |
| TscAI | CASTG | 7 cut(s) | 219, 256, 610, 1482, 1777, 1951, 2257 |
| TseFI | GTSAC | 4 cut(s) | 722, 1327, 1760, 2740 |
| TseI | GCWGC | 6 cut(s) | 515, 1147, 1291, 1294, 2039, 2664 |
| Tsp45I | GTSAC | 4 cut(s) | 722, 1327, 1760, 2740 |
| TspDTI | ATGAA | 8 cut(s) | 17, 582, 1064, 1398, 1949, 1965, 2537, 2603 |
| TspGWI | ACGGA | 1 cut(s) | 789 |
| TspRI | CASTG | 7 cut(s) | 219, 256, 610, 1482, 1777, 1951, 2257 |
| Vha464I | CTTAAG | 1 cut(s) | 788 |
| VneI | GTGCAC | 2 cut(s) | 1867, 2532 |
| VpaK11BI | GGWCC | 3 cut(s) | 1819, 2651, 2719 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 569, 1536 |
| XapI | RAATTY | 6 cut(s) | 47, 1245, 1466, 1586, 1810, 2757 |
| XceI | RCATGY | 2 cut(s) | 484, 1259 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1777 |
| XhoI | CTCGAG | 1 cut(s) | 165 |
| XmaJI | CCTAGG | 1 cut(s) | 410 |
| XmiI | GTMKAC | 1 cut(s) | 28 |
| XmnI | GAANNNNTTC | 3 cut(s) | 1245, 1956, 2591 |
| XspI | CTAG | 7 cut(s) | 411, 437, 944, 1548, 1907, 2414, 2570 |
| ZrmI | AGTACT | 1 cut(s) | 510 |