Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0001800

Physical Location & Seq

Genomic Coordinates Forward (+)

22342128 .. 22349427

Gene Structure 7300 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g24200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2415 bp

ATGCTGAAGCTTTTGTCGTCTTCCTCGTCATGCCTCCAAGGACGGCTCCACAGTCTCCGCTCCTGGCCGTCCGCTGAGCTGGGGTCGCCGGTTCTCCGAGTTCTCGGCTCGCTCGCCGGATTGACTCGCCCTAGCTCGGCTTGCCGAGCGTTTTATAGTTCGGACTCCGGCGACGGGTCTGACCAAGTGGTGGAAGTTGAGTTCATGGTGGCGGGGACCGAGGCCGAGTCTTCCTCAGCCATTGTGTCCACGAATCCCCGGCCCGAAGATTATCTTACGGTTATAGCTTTGCCATTGCCGCATAGACCGCTGTTTCCGGGGTTTTACATGCCAATTTATGTGAAGGATCCTAAACTGTTAGCAGCATTGCAAGAAAGCCGAAAGCGGCAAGCTCCATATGCCGGCGCTTTCCTTCTCAAGGATAAGCCGGGGACCGATCCCACTTTGGTATCAGGTTCCGAGACAGATAAAAACATACATGAATTGAAAGGGAAGGAGTTGTTTAATCGACTACATGAAGTTGGCACACTTGCGCAGATTTCAAGCATCCAAGGAGACCAAGTGGTCCTTATTGGTCATAGGCGACTTCGAATTACAGAGATGGTTGATGAAGAACCCCTGACTGTAAAAGTTGACCATCTGAAGGATAAACCATATAACAAGGATGATGATGTCATAAAAGCAACATCGTTTGAGGTTATATCAACCTTAAGAGATGTTCTCAAGATTAGTTCCCTTTGGAGAGATCATGTTCAGACATACACTCAGCATATAGGTGACTTTAATTTTCCAAGGTTAGCAGATTTTGGAGCAGCAATATCTGGTGCAAACAAATTGCAAAGTCAAGAAGTGCTTGAAGAGCTAGATGTGTATGAACGTTTAAAGCTCACTTTAGAAATAGTAAAGAAAGAGATAGAAATCAGCAAGATTCAGGAGTCTATTGCAAAAGCAATTGAAGAGAAGATAAGTGGTGAACAGCGCCGCTACTTGTTGAATGAGCAGCTTAAAGCCATTAAAAAGGAACTGGGTTTAGAGACAGATGACAAAACAGCGCTTAACGATAAGTTTAGGGAGAGGCTTGAACCAAACAGGGAAAACTGCCCACCTCATGTTTTACAAGTTATAGAGGAAGAGCTTACAAAACTGCAGTTGTTGGAAGCCAGTTCAAGTGAATTTAATGTGACACGTAATTACCTAGATTGGTTGACTTCAATTCCCTGGGGAAACTACAGTGATGAAAACTTTGATGTTATTCGGGCGCAAAAAATTCTTGATGAAGATCATTATGGATTAACTGATGTAAAGGAAAGGATATTAGAATTTATTGCCGTTGGAAAACTCAGAGGAACCTCTCAAGGAAAAATCATCTGTTTCTCTGGCCCACCTGGAGTGGGCAAAACCAGCATTGGTCGTTCAATTGCACGTGCCCTGAACCGTAACTTCTTCCGGTTTTCTGTGGGAGGGTTATCTGATGTTGCTGAAATTAAGGGGCATCGGCGAACCTACATTGGTGCTATGCCAGGAAAGATGGTGCAATGCCTTAAAAATGTGGGAACAGCTAACCCTTTAGTTCTGATTGATGAGATCGACAAGTTGGGAACGGGCCATGCCGGTGATCCGGCAAGTGCTTTGTTGGAGCTTCTTGATCCCGAGCAAAATGCTAATTTTCTAGACCATTATCTTGATGTTCCAATCGATCTATCAAAGGTTCTGTTTGTATGTACAGCCAATGTTGTAGACATGATACCACATCCACTCTTGGATAGAATGGAGGTCATTTCCATTGCTGGATATATCACTGATGAGAAAATGCACATTGCTAGAGACTATTTGGAGAAGACCGTACGTGACGCATGTGGCATTCAACCTGAACAGGTTGAAGTGACAGATGCAGCTCTTCTTGCCCTCATTGAAAATTATTGCCGAGAAGCAGGTGTTAGGAACCTTCAAAAGCACATAGAAAAGATCTACCGCAAGATAGCTCTCCAAATTGTTAGACAAGGAGCATCAGATGAACCAGTAGTTGCTGATCAAATTCAATCTCCCCCAGATGGGCCTGCAGTTGTTGAAGTTCAGCTTGTGGACACTGATGAAACCCAAGCAGAAGCTGTTGATAAGTTAGATCAAAGCGTGGTTGCTAGTAAGAACCAGACTGCAGCTGAATCAGTTGAGGGTAACGGTCATATGCATATTCCTGAAACCTCTGGAGAAGGAGTCACGATTCAGACTGATCTCCCAGTTGAATCTACAGTTGCAGAAGTTCGGGTTGCAGATGCTGATGAAACTGTGGATTCAAAGGATGCAAAAGAAACAGAGAAAATTCAGGAGGGTGAAACAACAAAAACAGTTGAGAAAGTTTTGGTTGACTCATCAAACTTGGATCGATTTTGTTGGCAAACCTGTCTTCCATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004176 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0006508 GO:0006515 GO:0006807 GO:0006996 GO:0007005 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019538 GO:0030163 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042623 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0051603 GO:0070011 GO:0070013 GO:0071704 GO:0071840 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA LON_substr_bdg Lon_C

Protein Analysis

805

Amino Acids

89.14

Weight (kDa)

5.05

Isoelectric Point (pI)

43.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LON_substr_bdg	PF02190	94 - 303	1.1e-36	ATP-dependent protease La (LON) substrate-binding domain
AAA	PF00004	456 - 593	6.8e-24	ATPase family associated with various cellular activities (AAA)
Lon_lid	PF22667	618 - 668	1.4e-19	Lon protease AAA+ ATPase lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001800)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05790 AT3G05790 AT5G26860
fragaria_vesca	FvH4_6g07420 FvH4_7g03300 FvH4_7g03300 FvH4_7g03300
malus_domestica	MD02G1284000.v1.1 MD04G1193200.v1.1 MD07G1050000.v1.1 MD12G1206100.v1.1
prunus_persica	Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.6G313300_v2.0.a1
pyrus_communis	pycom02g24200 pycom04g17100 pycom07g03580 pycom12g19400
rosa_chinensis	RchiOBHm_Chr1g0325611 RchiOBHm_Chr3g0457021
rosa_laevigata	RLG00000025251 RLG00000030117
rosa_multiflora	Rmu_sc0000488.1_g000001
rosa_roxburghii	Rroxscaffold_4G00324580 Rroxscaffold_6G00422260
rosa_rugosa	Rorug01G0053100 Rorug01G0053200 Rorug01G0053200 Rorug03G0018600 Rorug03G0018700
rosa_samantha	Rh1AG068800 Rh1CG069200 Rh1DG073900 Rh3AG078300 Rh3BG080200 Rh3CG080100 Rh3DG081900
rosa_wichuraiana	Rw1G005700 Rw3G006720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1922
AasI	GACNNNNNNGTC	1 cut(s)	563
Acc16I	TGCGCA	1 cut(s)	534
Acc36I	ACCTGC	1 cut(s)	1922
AccB7I	CCANNNNNTGG	1 cut(s)	190
AccBSI	CCGCTC	1 cut(s)	60
AccI	GTMKAC	1 cut(s)	1737
AciI	CCGC	8 cut(s)	58, 72, 212, 299, 308, 385, 982, 1972
AclI	AACGTT	1 cut(s)	877
AclWI	GGATC	6 cut(s)	341, 354, 431, 1610, 1640, 2388
AcoI	YGGCCR	1 cut(s)	65
AcsI	RAATTY	5 cut(s)	1172, 1266, 1319, 2034, 2319
AcuI	CTGAAG	2 cut(s)	26, 662
AcvI	CACGTG	1 cut(s)	1424
AfaI	GTAC	2 cut(s)	1723, 1845
AfeI	AGCGCT	1 cut(s)	1053
AfiI	CCNNNNNNNGG	9 cut(s)	136, 174, 190, 401, 418, 643, 1391, 2051, 2052
AflII	CTTAAG	1 cut(s)	709
AflIII	ACRYGT	1 cut(s)	1184
AjnI	CCWGG	4 cut(s)	62, 1217, 1384, 1519
AloI	GAACNNNNNNTCC	2 cut(s)	185, 217
Alw26I	GTCTC	5 cut(s)	59, 455, 549, 1028, 1818
AlwI	GGATC	6 cut(s)	341, 354, 431, 1610, 1640, 2388
AlwNI	CAGNNNCTG	2 cut(s)	2108, 2276
Ama87I	CYCGRG	1 cut(s)	1649
Aor51HI	AGCGCT	1 cut(s)	1053
AoxI	GGCC	6 cut(s)	65, 222, 260, 1378, 1603, 2054
ApeKI	GCWGC	5 cut(s)	362, 812, 1000, 1892, 2156
ApoI	RAATTY	5 cut(s)	1172, 1266, 1319, 2034, 2319
AspLEI	GCGC	5 cut(s)	407, 535, 981, 1054, 1261
AspS9I	GGNCC	7 cut(s)	216, 261, 432, 565, 1379, 1603, 2054
AsuC2I	CCSGG	3 cut(s)	259, 318, 429
AsuHPI	GGTGA	4 cut(s)	788, 983, 1625, 2342
AsuII	TTCGAA	1 cut(s)	589
AvaI	CYCGRG	1 cut(s)	1649
AvaII	GGWCC	3 cut(s)	216, 432, 565
BaeGI	GKGCMC	1 cut(s)	1429
BaeI	ACNNNNGTAYC	2 cut(s)	432, 465
BamHI	GGATCC	1 cut(s)	346
BbrPI	CACGTG	1 cut(s)	1424
BbsI	GAAGAC	4 cut(s)	12, 222, 1844, 2396
BbvCI	CCTCAGC	1 cut(s)	235
BbvI	GCAGC	5 cut(s)	374, 824, 1012, 1904, 2168
BccI	CCATC	4 cut(s)	595, 645, 1522, 2045
BceAI	ACGGC	3 cut(s)	52, 59, 1313
BcgI	CGANNNNNNTGC	2 cut(s)	1640, 1674
BciT130I	CCWGG	4 cut(s)	64, 1219, 1386, 1521
BclI	TGATCA	1 cut(s)	2029
BcnI	CCSGG	3 cut(s)	259, 318, 429
BcoDI	GTCTC	5 cut(s)	59, 455, 549, 1028, 1818
BfaI	CTAG	6 cut(s)	132, 863, 1196, 1670, 1821, 2139
BfmI	CTRYAG	5 cut(s)	1145, 1228, 2058, 2154, 2247
BfoI	RGCGCY	3 cut(s)	408, 982, 1055
BfrI	CTTAAG	1 cut(s)	709
BfuAI	ACCTGC	1 cut(s)	1922
BglII	AGATCT	1 cut(s)	1965
BisI	GCNGC	8 cut(s)	299, 363, 386, 813, 982, 1001, 1893, 2157
BlpI	GCTNAGC	1 cut(s)	75
BlsI	GCNGC	8 cut(s)	300, 364, 387, 814, 983, 1002, 1894, 2158
Bme1390I	CCNGG	7 cut(s)	64, 259, 318, 429, 1219, 1386, 1521
Bme18I	GGWCC	3 cut(s)	216, 432, 565
BmeT110I	CYCGRG	1 cut(s)	1649
BmgT120I	GGNCC	7 cut(s)	216, 261, 432, 565, 1379, 1603, 2054
BmiI	GGNNCC	7 cut(s)	47, 217, 348, 433, 457, 1348, 1943
BmrFI	CCNGG	7 cut(s)	64, 259, 318, 429, 1219, 1386, 1521
BmrI	ACTGGG	2 cut(s)	1034, 2231
BmsI	GCATC	6 cut(s)	555, 1501, 1879, 2015, 2263, 2290
BmuI	ACTGGG	2 cut(s)	1034, 2231
BpiI	GAAGAC	4 cut(s)	12, 222, 1844, 2396
BplI	GAGNNNNNCTC	4 cut(s)	218, 250, 705, 737
BpmI	CTGGAG	2 cut(s)	1407, 2226
Bpu10I	CCTNAGC	1 cut(s)	235
Bpu1102I	GCTNAGC	1 cut(s)	75
Bpu14I	TTCGAA	1 cut(s)	589
BpuEI	CTTGAG	3 cut(s)	401, 707, 1338
BpuMI	CCSGG	3 cut(s)	259, 318, 429
Bsa29I	ATCGAT	2 cut(s)	1695, 2383
BsaAI	YACGTR	3 cut(s)	1187, 1424, 1847
BsaBI	GATNNNNATC	2 cut(s)	917, 1278
BsaI	GGTCTC	1 cut(s)	549
BsaJI	CCNNGG	9 cut(s)	37, 219, 257, 317, 428, 550, 791, 1217, 1218
BsaWI	WCCGGW	1 cut(s)	1446
BsaXI	ACNNNNNCTCC	4 cut(s)	546, 576, 2199, 2229
Bsc4I	CCNNNNNNNGG	9 cut(s)	136, 174, 190, 401, 418, 643, 1391, 2051, 2052
Bse118I	RCCGGY	3 cut(s)	88, 401, 1610
Bse1I	ACTGG	4 cut(s)	1029, 1161, 2018, 2237
Bse3DI	GCAATG	5 cut(s)	293, 365, 1541, 1782, 1815
Bse8I	GATNNNNATC	2 cut(s)	917, 1278
BseBI	CCWGG	4 cut(s)	64, 1219, 1386, 1521
BseCI	ATCGAT	2 cut(s)	1695, 2383
BseDI	CCNNGG	9 cut(s)	37, 219, 257, 317, 428, 550, 791, 1217, 1218
BseGI	GGATG	4 cut(s)	546, 670, 1750, 2305
BseJI	GATNNNNATC	2 cut(s)	917, 1278
BseLI	CCNNNNNNNGG	9 cut(s)	136, 174, 190, 401, 418, 643, 1391, 2051, 2052
BseMI	GCAATG	5 cut(s)	293, 365, 1541, 1782, 1815
BseMII	CTCAG	4 cut(s)	66, 249, 779, 1354
BseNI	ACTGG	4 cut(s)	1029, 1161, 2018, 2237
BseSI	GKGCMC	1 cut(s)	1429
BseXI	GCAGC	5 cut(s)	374, 824, 1012, 1904, 2168
BseYI	CCCAGC	1 cut(s)	79
BshFI	GGCC	6 cut(s)	67, 224, 262, 1380, 1605, 2056
BshVI	ATCGAT	2 cut(s)	1695, 2383
BsiHKCI	CYCGRG	1 cut(s)	1649
BsiWI	CGTACG	1 cut(s)	1843
BslFI	GGGAC	2 cut(s)	229, 445
BslI	CCNNNNNNNGG	9 cut(s)	136, 174, 190, 401, 418, 643, 1391, 2051, 2052
BsmAI	GTCTC	5 cut(s)	59, 455, 549, 1028, 1818
BsmFI	GGGAC	2 cut(s)	229, 445
BsmI	GAATGC	1 cut(s)	1860
BsnI	GGCC	6 cut(s)	67, 224, 262, 1380, 1605, 2056
Bso31I	GGTCTC	1 cut(s)	549
BsoBI	CYCGRG	1 cut(s)	1649
Bsp119I	TTCGAA	1 cut(s)	589
Bsp1286I	GDGCHC	1 cut(s)	1429
Bsp1407I	TGTACA	1 cut(s)	1721
Bsp1720I	GCTNAGC	1 cut(s)	75
BspACI	CCGC	8 cut(s)	58, 72, 212, 299, 308, 385, 982, 1972
BspANI	GGCC	6 cut(s)	67, 224, 262, 1380, 1605, 2056
BspCNI	CTCAG	4 cut(s)	67, 248, 778, 1353
BspDI	ATCGAT	2 cut(s)	1695, 2383
BspLI	GGNNCC	7 cut(s)	47, 217, 348, 433, 457, 1348, 1943
BspMAI	CTGCAG	3 cut(s)	1149, 2062, 2158
BspMI	ACCTGC	1 cut(s)	1922
BspPI	GGATC	6 cut(s)	341, 354, 431, 1610, 1640, 2388
BspQI	GCTCTTC	3 cut(s)	852, 1125, 1902
BspT104I	TTCGAA	1 cut(s)	589
BspTI	CTTAAG	1 cut(s)	709
BspTNI	GGTCTC	1 cut(s)	549
BsrBI	CCGCTC	1 cut(s)	60
BsrDI	GCAATG	5 cut(s)	293, 365, 1541, 1782, 1815
BsrFI	RCCGGY	3 cut(s)	88, 401, 1610
BsrGI	TGTACA	1 cut(s)	1721
BsrI	ACTGG	4 cut(s)	1029, 1161, 2018, 2237
BssAI	RCCGGY	3 cut(s)	88, 401, 1610
BssECI	CCNNGG	9 cut(s)	37, 219, 257, 317, 428, 550, 791, 1217, 1218
BssT1I	CCWWGG	3 cut(s)	37, 550, 791
Bst2UI	CCWGG	4 cut(s)	64, 1219, 1386, 1521
Bst6I	CTCTTC	4 cut(s)	852, 951, 1125, 1902
BstAFI	CTTAAG	1 cut(s)	709
BstAUI	TGTACA	1 cut(s)	1721
BstBAI	YACGTR	3 cut(s)	1187, 1424, 1847
BstBI	TTCGAA	1 cut(s)	589
BstC8I	GCNNGC	6 cut(s)	110, 114, 142, 390, 403, 2058
BstDEI	CTNAG	4 cut(s)	75, 235, 765, 1340
BstENI	CCTNNNNNAGG	1 cut(s)	416
BstF5I	GGATG	4 cut(s)	546, 670, 1750, 2305
BstH2I	RGCGCY	3 cut(s)	408, 982, 1055
BstHHI	GCGC	5 cut(s)	407, 535, 981, 1054, 1261
BstMAI	GTCTC	5 cut(s)	59, 455, 549, 1028, 1818
BstMWI	GCNNNNNNNGC	9 cut(s)	85, 141, 146, 298, 307, 398, 859, 1401, 1901
BstNI	CCWGG	4 cut(s)	64, 1219, 1386, 1521
BstNSI	RCATGY	2 cut(s)	331, 1857
BstSCI	CCNGG	7 cut(s)	62, 257, 316, 427, 1217, 1384, 1519
BstSFI	CTRYAG	5 cut(s)	1145, 1228, 2058, 2154, 2247
BstSLI	GKGCMC	1 cut(s)	1429
BstV1I	GCAGC	5 cut(s)	374, 824, 1012, 1904, 2168
BstV2I	GAAGAC	4 cut(s)	12, 222, 1844, 2396
BstX2I	RGATCY	2 cut(s)	346, 1965
BstYI	RGATCY	2 cut(s)	346, 1965
Bsu15I	ATCGAT	2 cut(s)	1695, 2383
BsuRI	GGCC	6 cut(s)	67, 224, 262, 1380, 1605, 2056
BsuTUI	ATCGAT	2 cut(s)	1695, 2383
BtsCI	GGATG	4 cut(s)	546, 670, 1750, 2305
BtsIMutI	CAGTG	3 cut(s)	1237, 1797, 2085
BveI	ACCTGC	1 cut(s)	1922
Cac8I	GCNNGC	6 cut(s)	110, 114, 142, 390, 403, 2058
CaiI	CAGNNNCTG	2 cut(s)	2108, 2276
CfoI	GCGC	5 cut(s)	407, 535, 981, 1054, 1261
Cfr10I	RCCGGY	3 cut(s)	88, 401, 1610
Cfr13I	GGNCC	7 cut(s)	216, 261, 432, 565, 1379, 1603, 2054
ClaI	ATCGAT	2 cut(s)	1695, 2383
CseI	GACGC	1 cut(s)	1859
Csp6I	GTAC	2 cut(s)	1722, 1844
CviQI	GTAC	2 cut(s)	1722, 1844
DdeI	CTNAG	4 cut(s)	75, 235, 765, 1340
DraI	TTTAAA	1 cut(s)	882
DrdI	GACNNNNNNGTC	1 cut(s)	563
DseDI	GACNNNNNNGTC	1 cut(s)	563
EaeI	YGGCCR	1 cut(s)	65
Eam1104I	CTCTTC	4 cut(s)	852, 951, 1125, 1902
EarI	CTCTTC	4 cut(s)	852, 951, 1125, 1902
Eco130I	CCWWGG	3 cut(s)	37, 550, 791
Eco31I	GGTCTC	1 cut(s)	549
Eco47I	GGWCC	3 cut(s)	216, 432, 565
Eco47III	AGCGCT	1 cut(s)	1053
Eco57I	CTGAAG	2 cut(s)	26, 662
Eco72I	CACGTG	1 cut(s)	1424
Eco88I	CYCGRG	1 cut(s)	1649
EcoNI	CCTNNNNNAGG	1 cut(s)	416
EcoRII	CCWGG	4 cut(s)	62, 1217, 1384, 1519
EcoT14I	CCWWGG	3 cut(s)	37, 550, 791
EcoT22I	ATGCAT	1 cut(s)	2190
ErhI	CCWWGG	3 cut(s)	37, 550, 791
FalI	AAGNNNNNCTT	8 cut(s)	258, 290, 552, 584, 837, 869, 2061, 2093
FaqI	GGGAC	2 cut(s)	229, 445
FauI	CCCGC	1 cut(s)	205
FauNDI	CATATG	2 cut(s)	397, 2184
FbaI	TGATCA	1 cut(s)	2029
FblI	GTMKAC	1 cut(s)	1737
Fnu4HI	GCNGC	8 cut(s)	299, 363, 386, 813, 982, 1001, 1893, 2157
FokI	GGATG	4 cut(s)	533, 677, 1737, 2312
Fsp4HI	GCNGC	8 cut(s)	299, 363, 386, 813, 982, 1001, 1893, 2157
FspBI	CTAG	6 cut(s)	132, 863, 1196, 1670, 1821, 2139
FspI	TGCGCA	1 cut(s)	534
GlaI	GCGC	5 cut(s)	406, 534, 980, 1053, 1260
GluI	GCNGC	8 cut(s)	299, 363, 386, 813, 982, 1001, 1893, 2157
GsaI	CCCAGC	1 cut(s)	83
GsuI	CTGGAG	2 cut(s)	1407, 2226
HaeII	RGCGCY	3 cut(s)	408, 982, 1055
HaeIII	GGCC	6 cut(s)	67, 224, 262, 1380, 1605, 2056
HgaI	GACGC	1 cut(s)	1859
HhaI	GCGC	5 cut(s)	407, 535, 981, 1054, 1261
Hin6I	GCGC	5 cut(s)	405, 533, 979, 1052, 1259
HinP1I	GCGC	5 cut(s)	405, 533, 979, 1052, 1259
HincII	GTYRAC	3 cut(s)	634, 1206, 2365
HindII	GTYRAC	3 cut(s)	634, 1206, 2365
HindIII	AAGCTT	1 cut(s)	8
HphI	GGTGA	4 cut(s)	788, 983, 1625, 2342
Hpy166II	GTNNAC	7 cut(s)	249, 634, 974, 1206, 1738, 2083, 2365
Hpy8I	GTNNAC	7 cut(s)	249, 634, 974, 1206, 1738, 2083, 2365
Hpy99I	CGWCG	1 cut(s)	176
HpyAV	CCTTC	6 cut(s)	337, 422, 487, 637, 1955, 2204
HpyCH4IV	ACGT	4 cut(s)	877, 1186, 1423, 1846
HpyF10VI	GCNNNNNNNGC	9 cut(s)	85, 141, 146, 298, 307, 398, 859, 1401, 1901
HpyF3I	CTNAG	4 cut(s)	75, 235, 765, 1340
HpySE526I	ACGT	4 cut(s)	877, 1186, 1423, 1846
HspAI	GCGC	5 cut(s)	405, 533, 979, 1052, 1259
KroI	GCCGGC	1 cut(s)	401
KroNI	GCCGGC	1 cut(s)	403
Ksp22I	TGATCA	1 cut(s)	2029
LguI	GCTCTTC	3 cut(s)	852, 1125, 1902
LmnI	GCTCC	6 cut(s)	51, 65, 397, 809, 1636, 2003
Lsp1109I	GCAGC	5 cut(s)	374, 824, 1012, 1904, 2168
LweI	GCATC	6 cut(s)	555, 1501, 1879, 2015, 2263, 2290
MaeI	CTAG	6 cut(s)	132, 863, 1196, 1670, 1821, 2139
MaeII	ACGT	4 cut(s)	877, 1186, 1423, 1846
MaeIII	GTNAC	7 cut(s)	776, 1180, 1436, 1847, 1882, 2174, 2215
MbiI	CCGCTC	1 cut(s)	60
MfeI	CAATTG	2 cut(s)	951, 1416
MflI	RGATCY	2 cut(s)	346, 1965
MhlI	GDGCHC	1 cut(s)	1429
MlyI	GAGTC	6 cut(s)	118, 158, 236, 944, 2223, 2360
MmeI	TCCRAC	3 cut(s)	1134, 1312, 1614
Mph1103I	ATGCAT	1 cut(s)	2190
MroNI	GCCGGC	1 cut(s)	401
MslI	CAYNNNNRTG	2 cut(s)	774, 1611
MspA1I	CMGCKG	3 cut(s)	74, 310, 2159
MspCI	CTTAAG	1 cut(s)	709
MspR9I	CCNGG	7 cut(s)	64, 259, 318, 429, 1219, 1386, 1521
MunI	CAATTG	2 cut(s)	951, 1416
Mva1269I	GAATGC	1 cut(s)	1860
MvaI	CCWGG	4 cut(s)	64, 1219, 1386, 1521
MwoI	GCNNNNNNNGC	9 cut(s)	85, 141, 146, 298, 307, 398, 859, 1401, 1901
NaeI	GCCGGC	1 cut(s)	403
NciI	CCSGG	3 cut(s)	259, 318, 429
NdeI	CATATG	2 cut(s)	397, 2184
NgoMIV	GCCGGC	1 cut(s)	401
NlaIV	GGNNCC	7 cut(s)	47, 217, 348, 433, 457, 1348, 1943
NmeAIII	GCCGAG	5 cut(s)	84, 116, 170, 250, 1949
NmuCI	GTSAC	5 cut(s)	776, 1180, 1847, 1882, 2215
NsbI	TGCGCA	1 cut(s)	534
NsiI	ATGCAT	1 cut(s)	2190
NspI	RCATGY	2 cut(s)	331, 1857
NspV	TTCGAA	1 cut(s)	589
PaqCI	CACCTGC	1 cut(s)	1922
PasI	CCCWGGG	1 cut(s)	1218
PciSI	GCTCTTC	3 cut(s)	852, 1125, 1902
PcsI	WCGNNNNNNNCGW	1 cut(s)	23
PctI	GAATGC	1 cut(s)	1860
PdiI	GCCGGC	1 cut(s)	403
PfeI	GAWTC	6 cut(s)	253, 928, 2162, 2221, 2243, 2291
Pfl23II	CGTACG	1 cut(s)	1843
PflMI	CCANNNNNTGG	1 cut(s)	190
PkrI	GCNGC	8 cut(s)	300, 364, 387, 814, 983, 1002, 1894, 2158
PleI	GAGTC	6 cut(s)	118, 158, 235, 943, 2222, 2360
PmaCI	CACGTG	1 cut(s)	1424
PmlI	CACGTG	1 cut(s)	1424
PpsI	GAGTC	6 cut(s)	118, 158, 235, 943, 2222, 2360
Ppu21I	YACGTR	3 cut(s)	1187, 1424, 1847
Psp1406I	AACGTT	1 cut(s)	877
Psp6I	CCWGG	4 cut(s)	62, 1217, 1384, 1519
PspCI	CACGTG	1 cut(s)	1424
PspFI	CCCAGC	1 cut(s)	79
PspGI	CCWGG	4 cut(s)	62, 1217, 1384, 1519
PspLI	CGTACG	1 cut(s)	1843
PspN4I	GGNNCC	7 cut(s)	47, 217, 348, 433, 457, 1348, 1943
PspPI	GGNCC	7 cut(s)	216, 261, 432, 565, 1379, 1603, 2054
PstI	CTGCAG	3 cut(s)	1149, 2062, 2158
PstNI	CAGNNNCTG	2 cut(s)	2108, 2276
PsuI	RGATCY	2 cut(s)	346, 1965
PvuII	CAGCTG	1 cut(s)	2159
RsaI	GTAC	2 cut(s)	1723, 1845
RsaNI	GTAC	2 cut(s)	1722, 1844
RseI	CAYNNNNRTG	2 cut(s)	774, 1611
SapI	GCTCTTC	3 cut(s)	852, 1125, 1902
SatI	GCNGC	8 cut(s)	299, 363, 386, 813, 982, 1001, 1893, 2157
Sau96I	GGNCC	7 cut(s)	216, 261, 432, 565, 1379, 1603, 2054
SchI	GAGTC	6 cut(s)	118, 158, 236, 944, 2223, 2360
ScrFI	CCNGG	7 cut(s)	64, 259, 318, 429, 1219, 1386, 1521
SduI	GDGCHC	1 cut(s)	1429
SfaNI	GCATC	6 cut(s)	555, 1501, 1879, 2015, 2263, 2290
SfcI	CTRYAG	5 cut(s)	1145, 1228, 2058, 2154, 2247
SfuI	TTCGAA	1 cut(s)	589
SinI	GGWCC	3 cut(s)	216, 432, 565
SmiMI	CAYNNNNRTG	2 cut(s)	774, 1611
SmlI	CTYRAG	4 cut(s)	416, 709, 722, 1353
SmoI	CTYRAG	4 cut(s)	416, 709, 722, 1353
SsiI	CCGC	8 cut(s)	58, 72, 212, 299, 308, 385, 982, 1972
SspMI	CTAG	6 cut(s)	132, 863, 1196, 1670, 1821, 2139
StyD4I	CCNGG	7 cut(s)	62, 257, 316, 427, 1217, 1384, 1519
StyI	CCWWGG	3 cut(s)	37, 550, 791
TaiI	ACGT	4 cut(s)	880, 1189, 1426, 1849
TaqI	TCGA	5 cut(s)	508, 589, 1587, 1695, 2383
TaqII	GACCGA	2 cut(s)	233, 449
TatI	WGTACW	1 cut(s)	1721
TauI	GCSGC	3 cut(s)	301, 388, 984
TfiI	GAWTC	6 cut(s)	253, 928, 2162, 2221, 2243, 2291
TscAI	CASTG	3 cut(s)	1237, 1804, 2092
TseFI	GTSAC	5 cut(s)	776, 1180, 1847, 1882, 2215
TseI	GCWGC	5 cut(s)	362, 812, 1000, 1892, 2156
Tsp45I	GTSAC	5 cut(s)	776, 1180, 1847, 1882, 2215
TspRI	CASTG	3 cut(s)	1237, 1804, 2092
Van91I	CCANNNNNTGG	1 cut(s)	190
Vha464I	CTTAAG	1 cut(s)	709
VpaK11BI	GGWCC	3 cut(s)	216, 432, 565
XagI	CCTNNNNNAGG	1 cut(s)	416
XapI	RAATTY	5 cut(s)	1172, 1266, 1319, 2034, 2319
XbaI	TCTAGA	1 cut(s)	1669
XceI	RCATGY	2 cut(s)	331, 1857
XmiI	GTMKAC	1 cut(s)	1737
XspI	CTAG	6 cut(s)	132, 863, 1196, 1670, 1821, 2139
Zsp2I	ATGCAT	1 cut(s)	2190

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.