Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0001800

Physical Location & Seq

Genomic Coordinates Forward (+)

43391788 .. 43398570

Gene Structure 6783 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025251

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2988 bp

ATGTTGAAGCTTCTCTCTTCTTCCTCCTCGTGCCTACAGGCCGGAGCCCACAGTTTGGGCCCTAAATTGGGCGTCCGGCCCGAAATGACTTCGCCGTTACTCCGAGTTCTGAACTCCCTCACCGGCCGGAATCGGCCCAATATGGCTCGCCGGGCGTTTTTTTGCTCGGATTCAAGCGACGGCTCCGATCAAGTTGTTGAGTTGGAGGCCAAGGCTGGGACTGAGGGTGAGGCTGAGGCTAAGGCTTCTTCAGCTATAGTGTCCACAAACCCTAGACCAGAAGATTATCTAACGGTTTTAGCTTTGCCATTGCCGCATAGACCACTCTTTCCAGGGTTCTACATGCCAATTTATGTAAAGGATCCGAAATTGCTAGCGGCTTTACAGGAAAGCCGAAAACGGCAAGCTCCATATGCTGGAGCTTTTCTGGTGAAGGATGAGCCAGGGACTGATCCAAGTTTGGTATCAGGTTCTGATACAGAGAAAAGCATATATGAATTAAAAGGGAAAGCCTTGTTTAATCGATTGCATGAAGTTGGTACGCTTGCCCAGATATCTAGTATCCAAGGTGACCAAGTAGTGCTTATTGGTCATAGGCGACTTCGAATTACAGAGATGGTTGATGAGGATCCCCTGACTGTAAAAGTTGACCATCTCAAGAATAAACCATATAACAAGGATGATGATGTCATAAAGGCTACATCGTTTGAGGTTATAGCAACCTTAAGAGATGTTCTGAAGACTAGTTCTCTTTGGAGAGATCATGTTCAAACATACACTCAGCATATAGGTGACTTTAATTTTCCAAGGTTAGCAGATTTTGGAGCTGCAATATCTGGTGCAAACAAATTGCAGTGCCAACAAGTGCTTGAAGAGCTAGACGTGTACAAACGTTTAAATCTTACACTAGAATTGGTGAAGAAAGAGATGGAAATCAGCAAGCTTCAGGAAACTATTGCAAAAGCGATTGAAGAGAAGATAAGCCGTGAGCAACGCCGCTACTTGTTGAATGAGCAGCTTAAAGCCATAAAGAAGGAACTGGGATTAGAGACAGATGACAAGACAGCACTTTCTGCAAAGTTTAGGGAGAGGCTCGAACCAAACAAGGATAAATGTCCACCTCATGTTTTGCAAGTTATAGAAGAAGAGCTTACAAAACTACAGCTGCTGGAGGCCAGTTCGAGTGAATTTAACGTGACACGTAATTACCTAGATTGGTTGACGTCAATTCCCTGGGGAAATTACAGTAATGAGAACTTTGATGTTCTTCGGGCTGAAAAAATTCTTGATGAAGACCATTACGGATTAAACGATGTAAAGGAAAGGATATTGGAATTTATTGCTGTTGGAAAACTCAGAGGAATCTCACAAGGAAAAATCATTTGTCTCTCTGGCCCTCCTGGAGTTGGCAAAACCAGCATTGGTCGTTCAATTGCACGTGCATTGAATCGCAATTTCTTTCGATTTTCTGTTGGAGGGTTATCTGATGTCGCTGAAATCAAGGGACATCGCCGAACCTATATCGGTGCTATGCCAGGAAAGATGGTTCAATGCCTTAAAAATGTCGGAACAGCTAACCCTCTAGTTCTAATTGATGAGATCGACAAGTTGGGAATTGGACATGCTGGTGATCCGGCTAGCGCTTTGTTGGAGCTTCTTGATCCAGAGCAAAATGCTAATTTTCTAGATCATTATCTTGATGTTCCAATTGACCTAACAAAGGTTCTGTTTGTTTGTACAGCAAATGTTGTCGAGAATATACCAGCTCCTCTCTTGGATAGAATGGAGGTCATTTCCATAGCTGGGTATATTACTGATGAGAAAATGCACATTGCTAGAGACTATTTGGAGAAGACGACACGTGAAGCATGTGGCATCGAACCTGAACAGGTTGAAGTGACCAATGCAGCACTTCTTGCTCTCGTAGAAAATTATTGCCGAGAAGCAGGTGTTCGGAACCTCCAAAAGCACATAGAAAAGATCTACCGTAAGATAGCTTTGCAACTTGTTAGGCAAGGACAGTCAGATGAACCTGTAGATGCTGGCGAAGTACAGTCTCCCGTCAGTAAAACTGATCTCCCAGATGAACCTGCAGTTGTTGCAGATACTGATGAAGCCAAAGTAGAAGATATTGCTGGCGTTAAACAGTCTGCAGATGAATCAGCAGAGGGTAAAGACCATGGACATAATACTGAAACCTCTGTGGGAAGTGTCACTATCCCAGATGAAACTGGAGCTTCTGAACTTCAGGTTGCAGATACTGGTGAACTTATGCATTCAAAGGATACAGAAGAAACTCAGAAAATTCAGGAGGGTGAAGCCAAAAAACCAGTTGAGAAAGTGTTGGTTAATGAATCAAACTTGGGTGACTTTGTGGGCAAACCAGTTTTCCATGCGGAGCGCATCTATGAACAGACTCCGGTTGGAGTTGTCATGGGTCTTGCTTGGACTGCCATGGGTGGTTCCACCCTGTATATAGAGACCACTCAGGTTGAGGAAGGAGAGGGAAAAGGAGCGCTTCATGCTACTGGTCAACTTGGAGATGTTATGAAAGAAAGTACCCAAATTGCTCACACTGTGGCCAGAGCAATATTGCTCGAGAAAGAGCCAGATAACACTTTCTTTGCCGATTCCAAGCTTCATCTCCATGTGCCGGCAGGGGCCACACCAAAGGATGGGCCAAGTGCTGGTTGCACCATGATCACATCCATGCTGTCCATTGCCATGAAGAAGCCTGTGAAGAAGGATTTAGCAATGACAGGGGAAGTAACACTAACTGGCAGGATCCTTCCAATTGGCGGGGTTAAGGAGAAGACAATAGCAGCAAGACGGAGTGGAGTGAAGACCATCATATTCCCTGAAGCCAACAGGAAGGATTTTGATGAGCTTGCCCCTAATGTAAAAGAAGGTCTTGATGTTCACTTTGTAGATGACTACAATCAGATATTCGCTTTGGCTTTCAGTGATGACCAGAATCAAGAAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004176 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0006508 GO:0006515 GO:0006807 GO:0006996 GO:0007005 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019538 GO:0030163 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042623 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0051603 GO:0070011 GO:0070013 GO:0071704 GO:0071840 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA LON_substr_bdg Lon_C

Protein Analysis

996

Amino Acids

109.49

Weight (kDa)

5.33

Isoelectric Point (pI)

39.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LON_substr_bdg	PF02190	99 - 308	3.9e-36	ATP-dependent protease La (LON) substrate-binding domain
AAA	PF00004	461 - 598	7.2e-23	ATPase family associated with various cellular activities (AAA)
Lon_lid	PF22667	622 - 673	4.3e-18	Lon protease AAA+ ATPase lid domain
Lon_C	PF05362	783 - 987	8.4e-77	Lon protease (S16) C-terminal proteolytic domain
ChlI	PF13541	835 - 956	1e-05	Subunit ChlI of Mg-chelatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001800)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05790 AT3G05790 AT5G26860
fragaria_vesca	FvH4_6g07420 FvH4_7g03300 FvH4_7g03300 FvH4_7g03300
malus_domestica	MD02G1284000.v1.1 MD04G1193200.v1.1 MD07G1050000.v1.1 MD12G1206100.v1.1
prunus_persica	Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.6G313300_v2.0.a1
pyrus_communis	pycom02g24200 pycom04g17100 pycom07g03580 pycom12g19400
rosa_chinensis	RchiOBHm_Chr1g0325611 RchiOBHm_Chr3g0457021
rosa_laevigata	RLG00000025251 RLG00000030117
rosa_multiflora	Rmu_sc0000488.1_g000001
rosa_roxburghii	Rroxscaffold_4G00324580 Rroxscaffold_6G00422260
rosa_rugosa	Rorug01G0053100 Rorug01G0053200 Rorug01G0053200 Rorug03G0018600 Rorug03G0018700
rosa_samantha	Rh1AG068800 Rh1CG069200 Rh1DG073900 Rh3AG078300 Rh3BG080200 Rh3CG080100 Rh3DG081900
rosa_wichuraiana	Rw1G005700 Rw3G006720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1937
AatII	GACGTC	1 cut(s)	1226
Acc36I	ACCTGC	2 cut(s)	1937, 2098
AccB7I	CCANNNNNTGG	4 cut(s)	55, 416, 2675, 2687
AciI	CCGC	5 cut(s)	314, 377, 997, 2396, 2799
AclI	AACGTT	1 cut(s)	892
AclWI	GGATC	9 cut(s)	356, 369, 446, 623, 636, 1625, 1655, 2779, 2792
AcoI	YGGCCR	2 cut(s)	124, 2580
AcsI	RAATTY	4 cut(s)	1187, 1281, 1334, 2304
AcuI	CTGAAG	5 cut(s)	234, 758, 929, 2231, 2880
AcvI	CACGTG	2 cut(s)	1439, 1862
AcyI	GRCGYC	2 cut(s)	72, 1223
AdeI	CACNNNGTG	1 cut(s)	2578
AfaI	GTAC	5 cut(s)	541, 887, 1738, 2052, 2560
AfeI	AGCGCT	2 cut(s)	1642, 2517
AflII	CTTAAG	1 cut(s)	724
AflIII	ACRYGT	3 cut(s)	882, 1199, 1859
AhlI	ACTAGT	1 cut(s)	743
AjiI	CACGTC	1 cut(s)	883
AjnI	CCWGG	5 cut(s)	331, 442, 1232, 1399, 1534
AloI	GAACNNNNNNTCC	2 cut(s)	1530, 1562
Alw26I	GTCTC	5 cut(s)	1043, 1391, 1833, 2061, 2474
AlwI	GGATC	9 cut(s)	356, 369, 446, 623, 636, 1625, 1655, 2779, 2792
AlwNI	CAGNNNCTG	5 cut(s)	449, 473, 1168, 2108, 2261
Ama87I	CYCGRG	1 cut(s)	2597
Aor51HI	AGCGCT	2 cut(s)	1642, 2517
ApaI	GGGCCC	1 cut(s)	62
ApeKI	GCWGC	5 cut(s)	827, 1015, 1165, 1907, 2822
ApoI	RAATTY	4 cut(s)	1187, 1281, 1334, 2304
Asp700I	GAANNNNTTC	1 cut(s)	1281
AspLEI	GCGC	3 cut(s)	1643, 2403, 2518
AspS9I	GGNCC	7 cut(s)	58, 59, 78, 135, 1394, 2661, 2678
AsuC2I	CCSGG	1 cut(s)	152
AsuII	TTCGAA	1 cut(s)	604
AsuNHI	GCTAGC	2 cut(s)	373, 1637
AvaI	CYCGRG	1 cut(s)	2597
BaeGI	GKGCMC	1 cut(s)	62
BalI	TGGCCA	1 cut(s)	2582
BamHI	GGATCC	3 cut(s)	361, 628, 2784
BanII	GRGCYC	2 cut(s)	49, 62
BauI	CACGAG	1 cut(s)	28
BbrPI	CACGTG	2 cut(s)	1439, 1862
BbsI	GAAGAC	5 cut(s)	746, 1299, 1859, 2819, 2849
BbvCI	CCTCAGC	1 cut(s)	234
BbvI	GCAGC	5 cut(s)	814, 1027, 1152, 1919, 2834
BccI	CCATC	6 cut(s)	610, 660, 922, 1537, 2669, 2855
BceAI	ACGGC	4 cut(s)	79, 196, 416, 969
BciT130I	CCWGG	5 cut(s)	333, 444, 1234, 1401, 1536
BciVI	GTATCC	2 cut(s)	572, 2278
BclI	TGATCA	1 cut(s)	2700
BcnI	CCSGG	1 cut(s)	152
BcoDI	GTCTC	5 cut(s)	1043, 1391, 1833, 2061, 2474
BcuI	ACTAGT	1 cut(s)	743
BfmI	CTRYAG	6 cut(s)	35, 255, 1160, 2034, 2091, 2151
BfoI	RGCGCY	2 cut(s)	1644, 2519
BfrI	CTTAAG	1 cut(s)	724
BfuAI	ACCTGC	2 cut(s)	1937, 2098
BfuI	GTATCC	2 cut(s)	572, 2278
BglII	AGATCT	1 cut(s)	1980
BisI	GCNGC	8 cut(s)	314, 378, 828, 997, 1016, 1166, 1908, 2823
BlsI	GCNGC	8 cut(s)	315, 379, 829, 998, 1017, 1167, 1909, 2824
Bme1390I	CCNGG	6 cut(s)	152, 333, 444, 1234, 1401, 1536
BmeT110I	CYCGRG	1 cut(s)	2597
BmgBI	CACGTC	1 cut(s)	883
BmgT120I	GGNCC	7 cut(s)	58, 59, 78, 135, 1394, 2661, 2678
BmiI	GGNNCC	9 cut(s)	46, 60, 184, 363, 630, 1958, 2464, 2662, 2786
BmrFI	CCNGG	6 cut(s)	152, 333, 444, 1234, 1401, 1536
BmrI	ACTGGG	1 cut(s)	1049
BmsI	GCATC	3 cut(s)	1884, 2029, 2412
BmtI	GCTAGC	2 cut(s)	377, 1641
BmuI	ACTGGG	1 cut(s)	1049
BpiI	GAAGAC	5 cut(s)	746, 1299, 1859, 2819, 2849
BpmI	CTGGAG	4 cut(s)	438, 1190, 1422, 2253
Bpu10I	CCTNAGC	2 cut(s)	234, 240
Bpu14I	TTCGAA	1 cut(s)	604
BpuEI	CTTGAG	1 cut(s)	641
BpuMI	CCSGG	1 cut(s)	152
Bsa29I	ATCGAT	1 cut(s)	523
BsaAI	YACGTR	3 cut(s)	1202, 1439, 1862
BsaBI	GATNNNNATC	3 cut(s)	627, 932, 1692
BsaHI	GRCGYC	2 cut(s)	72, 1223
BsaI	GGTCTC	1 cut(s)	2474
BsaJI	CCNNGG	9 cut(s)	210, 332, 443, 565, 806, 1232, 1233, 2179, 2454
BsaWI	WCCGGW	1 cut(s)	2419
Bse118I	RCCGGY	2 cut(s)	122, 2653
Bse1I	ACTGG	8 cut(s)	1044, 1176, 2236, 2266, 2330, 2384, 2533, 2782
Bse3DI	GCAATG	4 cut(s)	308, 1830, 2718, 2760
Bse8I	GATNNNNATC	3 cut(s)	627, 932, 1692
BseBI	CCWGG	5 cut(s)	333, 444, 1234, 1401, 1536
BseCI	ATCGAT	1 cut(s)	523
BseDI	CCNNGG	9 cut(s)	210, 332, 443, 565, 806, 1232, 1233, 2179, 2454
BseGI	GGATG	4 cut(s)	442, 685, 2680, 2705
BseJI	GATNNNNATC	3 cut(s)	627, 932, 1692
BseMI	GCAATG	4 cut(s)	308, 1830, 2718, 2760
BseMII	CTCAG	6 cut(s)	213, 225, 794, 1369, 2312, 2501
BseNI	ACTGG	8 cut(s)	1044, 1176, 2236, 2266, 2330, 2384, 2533, 2782
BseRI	GAGGAG	2 cut(s)	16, 1758
BseSI	GKGCMC	1 cut(s)	62
BseX3I	CGGCCG	1 cut(s)	124
BseXI	GCAGC	5 cut(s)	814, 1027, 1152, 1919, 2834
BseYI	CCCAGC	2 cut(s)	215, 1802
Bsh1285I	CGRYCG	1 cut(s)	127
BshVI	ATCGAT	1 cut(s)	523
BsiEI	CGRYCG	1 cut(s)	127
BsiHKCI	CYCGRG	1 cut(s)	2597
BsiSI	CCGG	8 cut(s)	42, 76, 123, 127, 151, 1634, 2420, 2654
BslFI	GGGAC	3 cut(s)	232, 460, 1518
BsmAI	GTCTC	5 cut(s)	1043, 1391, 1833, 2061, 2474
BsmFI	GGGAC	3 cut(s)	232, 460, 1518
BsmI	GAATGC	1 cut(s)	2275
Bso31I	GGTCTC	1 cut(s)	2474
BsoBI	CYCGRG	1 cut(s)	2597
Bsp119I	TTCGAA	1 cut(s)	604
Bsp120I	GGGCCC	1 cut(s)	58
Bsp1286I	GDGCHC	2 cut(s)	49, 62
Bsp1407I	TGTACA	2 cut(s)	885, 1736
Bsp19I	CCATGG	2 cut(s)	2179, 2454
BspACI	CCGC	5 cut(s)	314, 377, 997, 2396, 2799
BspCNI	CTCAG	6 cut(s)	214, 226, 793, 1368, 2311, 2500
BspDI	ATCGAT	1 cut(s)	523
BspLI	GGNNCC	9 cut(s)	46, 60, 184, 363, 630, 1958, 2464, 2662, 2786
BspMAI	CTGCAG	2 cut(s)	2095, 2155
BspMI	ACCTGC	2 cut(s)	1937, 2098
BspOI	GCTAGC	2 cut(s)	377, 1641
BspPI	GGATC	9 cut(s)	356, 369, 446, 623, 636, 1625, 1655, 2779, 2792
BspQI	GCTCTTC	2 cut(s)	867, 1140
BspT104I	TTCGAA	1 cut(s)	604
BspTI	CTTAAG	1 cut(s)	724
BspTNI	GGTCTC	1 cut(s)	2474
BsrDI	GCAATG	4 cut(s)	308, 1830, 2718, 2760
BsrFI	RCCGGY	2 cut(s)	122, 2653
BsrGI	TGTACA	2 cut(s)	885, 1736
BsrI	ACTGG	8 cut(s)	1044, 1176, 2236, 2266, 2330, 2384, 2533, 2782
BssAI	RCCGGY	2 cut(s)	122, 2653
BssECI	CCNNGG	9 cut(s)	210, 332, 443, 565, 806, 1232, 1233, 2179, 2454
BssNI	GRCGYC	2 cut(s)	72, 1223
BssSI	CACGAG	1 cut(s)	28
BssT1I	CCWWGG	5 cut(s)	210, 565, 806, 2179, 2454
Bst2BI	CACGAG	1 cut(s)	28
Bst2UI	CCWGG	5 cut(s)	333, 444, 1234, 1401, 1536
Bst4CI	ACNGT	9 cut(s)	53, 295, 640, 1247, 1988, 2022, 2055, 2148, 2578
Bst6I	CTCTTC	4 cut(s)	22, 867, 966, 1140
BstACI	GRCGYC	2 cut(s)	72, 1223
BstAFI	CTTAAG	1 cut(s)	724
BstAPI	GCANNNNNTGC	3 cut(s)	1073, 1916, 2099
BstAUI	TGTACA	2 cut(s)	885, 1736
BstBAI	YACGTR	3 cut(s)	1202, 1439, 1862
BstBI	TTCGAA	1 cut(s)	604
BstDEI	CTNAG	7 cut(s)	222, 234, 240, 780, 1355, 2298, 2487
BstDSI	CCRYGG	2 cut(s)	2179, 2454
BstEII	GGTNACC	1 cut(s)	569
BstENI	CCTNNNNNAGG	1 cut(s)	1718
BstF5I	GGATG	4 cut(s)	442, 685, 2680, 2705
BstH2I	RGCGCY	2 cut(s)	1644, 2519
BstHHI	GCGC	3 cut(s)	1643, 2403, 2518
BstMAI	GTCTC	5 cut(s)	1043, 1391, 1833, 2061, 2474
BstMCI	CGRYCG	1 cut(s)	127
BstNI	CCWGG	5 cut(s)	333, 444, 1234, 1401, 1536
BstNSI	RCATGY	3 cut(s)	346, 1625, 1872
BstPI	GGTNACC	1 cut(s)	569
BstSCI	CCNGG	6 cut(s)	150, 331, 442, 1232, 1399, 1534
BstSFI	CTRYAG	6 cut(s)	35, 255, 1160, 2034, 2091, 2151
BstSLI	GKGCMC	1 cut(s)	62
BstV1I	GCAGC	5 cut(s)	814, 1027, 1152, 1919, 2834
BstV2I	GAAGAC	5 cut(s)	746, 1299, 1859, 2819, 2849
BstX2I	RGATCY	4 cut(s)	361, 628, 1980, 2784
BstYI	RGATCY	4 cut(s)	361, 628, 1980, 2784
BstZI	CGGCCG	1 cut(s)	124
Bsu15I	ATCGAT	1 cut(s)	523
BsuI	GTATCC	2 cut(s)	572, 2278
BsuTUI	ATCGAT	1 cut(s)	523
BtgI	CCRYGG	2 cut(s)	2179, 2454
BtgZI	GCGATG	1 cut(s)	1493
BtrI	CACGTC	1 cut(s)	883
BtsCI	GGATG	4 cut(s)	442, 685, 2680, 2705
BtsI	GCAGTG	1 cut(s)	860
BtsIMutI	CAGTG	3 cut(s)	860, 2574, 2968
BveI	ACCTGC	2 cut(s)	1937, 2098
CaiI	CAGNNNCTG	5 cut(s)	449, 473, 1168, 2108, 2261
CfoI	GCGC	3 cut(s)	1643, 2403, 2518
Cfr10I	RCCGGY	2 cut(s)	122, 2653
Cfr13I	GGNCC	7 cut(s)	58, 59, 78, 135, 1394, 2661, 2678
ClaI	ATCGAT	1 cut(s)	523
CseI	GACGC	1 cut(s)	61
Csp6I	GTAC	5 cut(s)	540, 886, 1737, 2051, 2559
CspCI	CAANNNNNGTGG	2 cut(s)	2473, 2508
CviQI	GTAC	5 cut(s)	540, 886, 1737, 2051, 2559
DdeI	CTNAG	7 cut(s)	222, 234, 240, 780, 1355, 2298, 2487
DraI	TTTAAA	1 cut(s)	897
DraIII	CACNNNGTG	1 cut(s)	2578
EaeI	YGGCCR	2 cut(s)	124, 2580
EagI	CGGCCG	1 cut(s)	124
Eam1104I	CTCTTC	4 cut(s)	22, 867, 966, 1140
EarI	CTCTTC	4 cut(s)	22, 867, 966, 1140
EclXI	CGGCCG	1 cut(s)	124
Eco130I	CCWWGG	5 cut(s)	210, 565, 806, 2179, 2454
Eco24I	GRGCYC	2 cut(s)	49, 62
Eco31I	GGTCTC	1 cut(s)	2474
Eco32I	GATATC	1 cut(s)	555
Eco47III	AGCGCT	2 cut(s)	1642, 2517
Eco52I	CGGCCG	1 cut(s)	124
Eco57I	CTGAAG	5 cut(s)	234, 758, 929, 2231, 2880
Eco72I	CACGTG	2 cut(s)	1439, 1862
Eco88I	CYCGRG	1 cut(s)	2597
Eco91I	GGTNACC	1 cut(s)	569
EcoNI	CCTNNNNNAGG	1 cut(s)	1718
EcoO109I	RGGNCCY	1 cut(s)	59
EcoO65I	GGTNACC	1 cut(s)	569
EcoRII	CCWGG	5 cut(s)	331, 442, 1232, 1399, 1534
EcoRV	GATATC	1 cut(s)	555
EcoT14I	CCWWGG	5 cut(s)	210, 565, 806, 2179, 2454
EcoT22I	ATGCAT	1 cut(s)	2277
EcoT38I	GRGCYC	2 cut(s)	49, 62
ErhI	CCWWGG	5 cut(s)	210, 565, 806, 2179, 2454
FalI	AAGNNNNNCTT	8 cut(s)	567, 599, 1134, 1166, 2502, 2534, 2895, 2927
FaqI	GGGAC	3 cut(s)	232, 460, 1518
FauI	CCCGC	1 cut(s)	2792
FauNDI	CATATG	1 cut(s)	412
FbaI	TGATCA	1 cut(s)	2700
Fnu4HI	GCNGC	8 cut(s)	314, 378, 828, 997, 1016, 1166, 1908, 2823
FokI	GGATG	4 cut(s)	449, 692, 2687, 2692
FriOI	GRGCYC	2 cut(s)	49, 62
Fsp4HI	GCNGC	8 cut(s)	314, 378, 828, 997, 1016, 1166, 1908, 2823
GlaI	GCGC	3 cut(s)	1642, 2402, 2517
GluI	GCNGC	8 cut(s)	314, 378, 828, 997, 1016, 1166, 1908, 2823
GsaI	CCCAGC	2 cut(s)	219, 1806
GsuI	CTGGAG	4 cut(s)	438, 1190, 1422, 2253
HaeII	RGCGCY	2 cut(s)	1644, 2519
HapII	CCGG	8 cut(s)	42, 76, 123, 127, 151, 1634, 2420, 2654
HgaI	GACGC	1 cut(s)	61
HhaI	GCGC	3 cut(s)	1643, 2403, 2518
Hin1I	GRCGYC	2 cut(s)	72, 1223
Hin6I	GCGC	3 cut(s)	1641, 2401, 2516
HinP1I	GCGC	3 cut(s)	1641, 2401, 2516
HincII	GTYRAC	3 cut(s)	649, 1221, 2534
HindII	GTYRAC	3 cut(s)	649, 1221, 2534
HindIII	AAGCTT	3 cut(s)	8, 941, 2636
HinfI	GANTC	9 cut(s)	130, 170, 1362, 1447, 2159, 2354, 2416, 2630, 2974
HpaII	CCGG	8 cut(s)	42, 76, 123, 127, 151, 1634, 2420, 2654
Hpy166II	GTNNAC	8 cut(s)	264, 649, 886, 1118, 1221, 2267, 2534, 2920
Hpy8I	GTNNAC	8 cut(s)	264, 649, 886, 1118, 1221, 2267, 2534, 2920
Hpy99I	CGWCG	1 cut(s)	182
HpyAV	CCTTC	7 cut(s)	427, 1027, 2492, 2737, 2798, 2866, 2900
HpyCH4III	ACNGT	9 cut(s)	53, 295, 640, 1247, 1988, 2022, 2055, 2148, 2578
HpyCH4IV	ACGT	7 cut(s)	882, 892, 1194, 1201, 1223, 1438, 1861
HpyF3I	CTNAG	7 cut(s)	222, 234, 240, 780, 1355, 2298, 2487
HpySE526I	ACGT	7 cut(s)	882, 892, 1194, 1201, 1223, 1438, 1861
Hsp92I	GRCGYC	2 cut(s)	72, 1223
HspAI	GCGC	3 cut(s)	1641, 2401, 2516
KroI	GCCGGC	1 cut(s)	2653
KroNI	GCCGGC	1 cut(s)	2655
Ksp22I	TGATCA	1 cut(s)	2700
LguI	GCTCTTC	2 cut(s)	867, 1140
Lsp1109I	GCAGC	5 cut(s)	814, 1027, 1152, 1919, 2834
LweI	GCATC	3 cut(s)	1884, 2029, 2412
MaeII	ACGT	7 cut(s)	882, 892, 1194, 1201, 1223, 1438, 1861
MaeIII	GTNAC	8 cut(s)	96, 569, 791, 1195, 1897, 2212, 2366, 2767
MfeI	CAATTG	3 cut(s)	1431, 1707, 2793
MflI	RGATCY	4 cut(s)	361, 628, 1980, 2784
MhlI	GDGCHC	2 cut(s)	49, 62
MlsI	TGGCCA	1 cut(s)	2582
MluNI	TGGCCA	1 cut(s)	2582
MlyI	GAGTC	1 cut(s)	2410
MmeI	TCCRAC	6 cut(s)	183, 1327, 1453, 1546, 1629, 2404
Mox20I	TGGCCA	1 cut(s)	2582
Mph1103I	ATGCAT	1 cut(s)	2277
MroNI	GCCGGC	1 cut(s)	2653
MroXI	GAANNNNTTC	1 cut(s)	1281
MscI	TGGCCA	1 cut(s)	2582
MslI	CAYNNNNRTG	5 cut(s)	789, 1626, 2646, 2708, 2723
Msp20I	TGGCCA	1 cut(s)	2582
MspA1I	CMGCKG	1 cut(s)	1165
MspCI	CTTAAG	1 cut(s)	724
MspI	CCGG	8 cut(s)	42, 76, 123, 127, 151, 1634, 2420, 2654
MspR9I	CCNGG	6 cut(s)	152, 333, 444, 1234, 1401, 1536
MunI	CAATTG	3 cut(s)	1431, 1707, 2793
Mva1269I	GAATGC	1 cut(s)	2275
MvaI	CCWGG	5 cut(s)	333, 444, 1234, 1401, 1536
NaeI	GCCGGC	1 cut(s)	2655
NciI	CCSGG	1 cut(s)	152
NcoI	CCATGG	2 cut(s)	2179, 2454
NdeI	CATATG	1 cut(s)	412
NgoMIV	GCCGGC	1 cut(s)	2653
NheI	GCTAGC	2 cut(s)	373, 1637
NlaIV	GGNNCC	9 cut(s)	46, 60, 184, 363, 630, 1958, 2464, 2662, 2786
NmeAIII	GCCGAG	1 cut(s)	1964
NmuCI	GTSAC	6 cut(s)	569, 791, 1195, 1897, 2212, 2366
NsiI	ATGCAT	1 cut(s)	2277
NspI	RCATGY	3 cut(s)	346, 1625, 1872
NspV	TTCGAA	1 cut(s)	604
PaeR7I	CTCGAG	1 cut(s)	2597
PaqCI	CACCTGC	1 cut(s)	1937
PasI	CCCWGGG	1 cut(s)	1233
PciSI	GCTCTTC	2 cut(s)	867, 1140
PcsI	WCGNNNNNNNCGW	1 cut(s)	1308
PctI	GAATGC	1 cut(s)	2275
PdiI	GCCGGC	1 cut(s)	2655
PdmI	GAANNNNTTC	1 cut(s)	1281
PfeI	GAWTC	8 cut(s)	130, 170, 1362, 1447, 2159, 2354, 2630, 2974
PflMI	CCANNNNNTGG	4 cut(s)	55, 416, 2675, 2687
PfoI	TCCNGGA	1 cut(s)	1399
PkrI	GCNGC	8 cut(s)	315, 379, 829, 998, 1017, 1167, 1909, 2824
PleI	GAGTC	1 cut(s)	2410
PmaCI	CACGTG	2 cut(s)	1439, 1862
PmlI	CACGTG	2 cut(s)	1439, 1862
PpsI	GAGTC	1 cut(s)	2410
Ppu21I	YACGTR	3 cut(s)	1202, 1439, 1862
Psp1406I	AACGTT	1 cut(s)	892
Psp6I	CCWGG	5 cut(s)	331, 442, 1232, 1399, 1534
PspCI	CACGTG	2 cut(s)	1439, 1862
PspEI	GGTNACC	1 cut(s)	569
PspFI	CCCAGC	2 cut(s)	215, 1802
PspGI	CCWGG	5 cut(s)	331, 442, 1232, 1399, 1534
PspN4I	GGNNCC	9 cut(s)	46, 60, 184, 363, 630, 1958, 2464, 2662, 2786
PspOMI	GGGCCC	1 cut(s)	58
PspPI	GGNCC	7 cut(s)	58, 59, 78, 135, 1394, 2661, 2678
PstI	CTGCAG	2 cut(s)	2095, 2155
PstNI	CAGNNNCTG	5 cut(s)	449, 473, 1168, 2108, 2261
PsuI	RGATCY	4 cut(s)	361, 628, 1980, 2784
PvuII	CAGCTG	1 cut(s)	1165
RsaI	GTAC	5 cut(s)	541, 887, 1738, 2052, 2560
RsaNI	GTAC	5 cut(s)	540, 886, 1737, 2051, 2559
RseI	CAYNNNNRTG	5 cut(s)	789, 1626, 2646, 2708, 2723
SapI	GCTCTTC	2 cut(s)	867, 1140
SatI	GCNGC	8 cut(s)	314, 378, 828, 997, 1016, 1166, 1908, 2823
Sau96I	GGNCC	7 cut(s)	58, 59, 78, 135, 1394, 2661, 2678
SchI	GAGTC	1 cut(s)	2410
ScrFI	CCNGG	6 cut(s)	152, 333, 444, 1234, 1401, 1536
SduI	GDGCHC	2 cut(s)	49, 62
SfaNI	GCATC	3 cut(s)	1884, 2029, 2412
SfcI	CTRYAG	6 cut(s)	35, 255, 1160, 2034, 2091, 2151
Sfr274I	CTCGAG	1 cut(s)	2597
SfuI	TTCGAA	1 cut(s)	604
SlaI	CTCGAG	1 cut(s)	2597
SmiMI	CAYNNNNRTG	5 cut(s)	789, 1626, 2646, 2708, 2723
SmlI	CTYRAG	3 cut(s)	656, 724, 2597
SmoI	CTYRAG	3 cut(s)	656, 724, 2597
SpeI	ACTAGT	1 cut(s)	743
SsiI	CCGC	5 cut(s)	314, 377, 997, 2396, 2799
SspI	AATATT	1 cut(s)	2592
StyD4I	CCNGG	6 cut(s)	150, 331, 442, 1232, 1399, 1534
StyI	CCWWGG	5 cut(s)	210, 565, 806, 2179, 2454
TaaI	ACNGT	9 cut(s)	53, 295, 640, 1247, 1988, 2022, 2055, 2148, 2578
TaiI	ACGT	7 cut(s)	885, 895, 1197, 1204, 1226, 1441, 1864
TaqI	TCGA	9 cut(s)	523, 604, 1095, 1181, 1462, 1602, 1752, 1878, 2598
TatI	WGTACW	3 cut(s)	885, 1736, 2050
TauI	GCSGC	3 cut(s)	316, 380, 999
TfiI	GAWTC	8 cut(s)	130, 170, 1362, 1447, 2159, 2354, 2630, 2974
TscAI	CASTG	3 cut(s)	860, 2581, 2968
TseFI	GTSAC	6 cut(s)	569, 791, 1195, 1897, 2212, 2366
TseI	GCWGC	5 cut(s)	827, 1015, 1165, 1907, 2822
Tsp45I	GTSAC	6 cut(s)	569, 791, 1195, 1897, 2212, 2366
TspGWI	ACGGA	2 cut(s)	1317, 2845
TspRI	CASTG	3 cut(s)	860, 2581, 2968
Van91I	CCANNNNNTGG	4 cut(s)	55, 416, 2675, 2687
Vha464I	CTTAAG	1 cut(s)	724
XagI	CCTNNNNNAGG	1 cut(s)	1718
XapI	RAATTY	4 cut(s)	1187, 1281, 1334, 2304
XbaI	TCTAGA	1 cut(s)	1684
XceI	RCATGY	3 cut(s)	346, 1625, 1872
XhoI	CTCGAG	1 cut(s)	2597
XmnI	GAANNNNTTC	1 cut(s)	1281
ZraI	GACGTC	1 cut(s)	1224
Zsp2I	ATGCAT	1 cut(s)	2277

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.