Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0001800

Physical Location & Seq

Genomic Coordinates Forward (+)

56095960 .. 56103680

Gene Structure 7721 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00324580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2640 bp

ATGCTGAAACTCCTCTCTCCTTCTTCCCCGGCGGCCTCACCGTTTCTCCGAGCCTTCAAGGGGTTAGGAAATGAAGCAGAGGCTGAGTCCAAGTCGTCATCATCATCAGCTATCATACCCACAAATCTTAATCCTGAAGATTATGTAAAGGTTTTAGCATTGCCATTGCCGCATAGACCACTGTTTCCGGGTTTCTACATGCCAATATATGTGAAGGATCCTAAACTATTAGCAGCTCTACAGGAAAGTAGAAACCGGCAAGTTCCATATGCCGGTGCTTTCCTTGTCAAGGATGAACCAGGGACCGACCCTAGTTCTGAGACGGAGAATAGCATTCCTGATTTGAAAGGGAAAGAGTTGTTTGATCGTCTGCATGAAGTTGGTACGCTTGCCCAGATTTCAAGCATTCAAGGAGATCAAGTAGTCCTAATCGGCCATAGGCGACTTCAAATTACAGAGATGGTTGATGAGGATCCCGTGACTGATAAGCCATATGACAAGGATGACGATGTTATAAAGGCAACATCATATGAGCTTATATCAACTCTAAGAGATGTTCTGGAGACTAGTGTTCTTTGGAGAGATCATGCTCAAACATATACTCAGCACATAAGTGATTTTAATTTTACAAGGATAGCAGATTTTGGGGCTGCTATATCTGGTGCAAACAAATTGCAATGCCAAAAAGTACTTGAAGAGCTAGATGTGCATGAACGTTTAAAGCTCACACTAGAATTAGTGAAGAAAGAGATAGAGATCAGTAAGATTCAGGCATCTCTTATGAAAAAAATGGAAGAGAAAATGCTTTCTGAACAGCGTCGCAAATTGTTAAATGAGCAGCTTAAAGCAATAAAGAAGGAACTGGGATTAGAGGCTGATGATAGAACAGCGCTTTGTGAAAAGTTTAGGGAGAGGCTTGAACCAAATAGGGAAAAATGCCCACCTAATGTTATGCAAGTCATAGAAGAAGAGCTTAGCAAAATGCAGCTGTTGGATGCCAGTTCAAGTGAATTTAATGTAACACGTAATTATCTAGATTGGTTGATTTCACTTCCCTGGGGAAATTACAGTGATGAGAATTTTGATGTTCTTCGGGCGCAGAAAGTTCTCGATGAAGATCATTATGGATTAACTGAAGTAAAAGAAAGGATATTGGAATTTATTGCTGTTGGAAAACTCAGAGGAATGTCAGAAGGAAAAATCATCTGTCTCGCTGGCCCACCTGGAGTAGGAAAAACCAGCATTGGTCGTTCAATTGCACATGCCTTGAACCGTAATTTCTTTCGATTTTCTGTAGGAGGGTTAACTGACGCAGCTGAAATTAAGGGGCATCGTCGAACCTACGTTGGTGCCATGCCAGGAAAGATGGTACAATGCCTTAAAACTGTGGGAACAGCTAACCCTCTGGTTCTGATCGATGAGATTGACAAGTTGGGAAGGGGGCATGCTGGTCATCCGGCCAGTGCATTGTTGGAGCTTCTAGATCCAGAGCAAAATGCTAATTTTCTAGACCATTATCTTGATGTTCCAATTGACCTATCCAAGGTTCTGTTTGTTTGTACAGCAAATGTTGTGCACATGATTCCAACTCCTCTGTTGGATAGAATGGAGGTCGTTTCCATCGCTGGGTATATTACTGATGAGAAAATCCACATTGCTAGAGAGTATTTGGAGAAGACCACACGTGAAGCATGTGGCATTGAACCTGAACAGGTTGAGGTGACCGATGCAGCTCTTCTTGCACTCATTGAAAATTATTGCCGGGAAGCAGGTGTTAGGAACCTCCAGCAGCACATAGAAAGAATCTACCGCAAGATAGCTCTGCAACTTGTTAGACAAGGAGCAGCAAATCAACCGGCAGTTGGTGATAAAATACAGTCTCTCTCAGATGAACCTGCAGTTGTTGAAGTTGCAGATAGTGATGAACATGTTGTTGGTTCAAAGGTTCGAAGAGATACTAGAAATATTCCCAAAAGTGAAGCAACAAAAACAGTTGAGAAAGTGTTGGTTGACTCATCAAACTTAACTGATTTTGTTGGGAAACCTGTTTTCCATGCTGAACACATCTACGAACAGACTCCCGTTGGAGTTGTTATGGGTCTTGCTTGGACTGCCATGGGTGGTTGCCCCTTGTACATAGAGACCACTCAGGTTGAGGAAGCTCACGGGAAAGGAGCACTTCAAGTCACAGGCCAACTTGGGGATGTCATGAGAGAAAGTGCCCATATTGCTCACACTGTTGCCAGATCGATATTGCTTGATAAAGAACCAGATAACACTTTCTTTGCCGATTCCAAGCTTCATCTCCATATTCCTGCAGGGGCTATACCTAAGGATGGGCCCAGTGCTGGTTGTACTATGATAACATCCATGCTCTCCCTTGCCATGAAGAAGCCTGTCAACAAGGATTTAGCAATGACTGGGGAAGTAACACTAACCGGGAGGATCCTTCCAATTGGCGGGGTAAAGGAGAAAACAATAGCAGCGAGGAGGGGCGGAGTGAAGACCATCATATTCCCTTCAGCTAACAAGAGGGATTATGAGGAGCTTACCCCCAATGTAAAGGAAGGCCTGGATGTTTACTTTGTAGATGACTACAGTCAGATATTCAACTTGGCTTTCGGTGATCACGAAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004176 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0006508 GO:0006515 GO:0006807 GO:0006996 GO:0007005 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019538 GO:0030163 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042623 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0051603 GO:0070011 GO:0070013 GO:0071704 GO:0071840 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA LON_substr_bdg Lon_C

Protein Analysis

879

Amino Acids

97.47

Weight (kDa)

5.53

Isoelectric Point (pI)

41.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LON_substr_bdg	PF02190	51 - 249	2.4e-31	ATP-dependent protease La (LON) substrate-binding domain
AAA	PF00004	402 - 539	1.6e-23	ATPase family associated with various cellular activities (AAA)
AAA_5	PF07728	402 - 480	6.6e-07	AAA domain (dynein-related subfamily)
Lon_lid	PF22667	563 - 614	3.2e-18	Lon protease AAA+ ATPase lid domain
Lon_C	PF05362	670 - 874	1.8e-74	Lon protease (S16) C-terminal proteolytic domain
ChlI	PF13541	718 - 843	9.7e-06	Subunit ChlI of Mg-chelatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0001800)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05790 AT3G05790 AT5G26860
fragaria_vesca	FvH4_6g07420 FvH4_7g03300 FvH4_7g03300 FvH4_7g03300
malus_domestica	MD02G1284000.v1.1 MD04G1193200.v1.1 MD07G1050000.v1.1 MD12G1206100.v1.1
prunus_persica	Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.6G313300_v2.0.a1
pyrus_communis	pycom02g24200 pycom04g17100 pycom07g03580 pycom12g19400
rosa_chinensis	RchiOBHm_Chr1g0325611 RchiOBHm_Chr3g0457021
rosa_laevigata	RLG00000025251 RLG00000030117
rosa_multiflora	Rmu_sc0000488.1_g000001
rosa_roxburghii	Rroxscaffold_4G00324580 Rroxscaffold_6G00422260
rosa_rugosa	Rorug01G0053100 Rorug01G0053200 Rorug01G0053200 Rorug03G0018600 Rorug03G0018700
rosa_samantha	Rh1AG068800 Rh1CG069200 Rh1DG073900 Rh3AG078300 Rh3BG080200 Rh3CG080100 Rh3DG081900
rosa_wichuraiana	Rw1G005700 Rw3G006720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	515
AarI	CACCTGC	1 cut(s)	1760
Acc36I	ACCTGC	2 cut(s)	1760, 1903
AccB1I	GGYRCC	1 cut(s)	1349
AciI	CCGC	5 cut(s)	32, 170, 1810, 2460, 2496
AclI	AACGTT	1 cut(s)	715
AclWI	GGATC	7 cut(s)	212, 225, 467, 480, 1478, 2440, 2453
AcoI	YGGCCR	2 cut(s)	433, 1458
AcsI	RAATTY	3 cut(s)	1010, 1078, 1157
AcuI	CTGAAG	3 cut(s)	156, 1155, 2505
AcvI	CACGTG	1 cut(s)	1685
AfaI	GTAC	6 cut(s)	385, 690, 1371, 1561, 2135, 2356
AfeI	AGCGCT	1 cut(s)	891
AflIII	ACRYGT	3 cut(s)	1022, 1682, 1927
AhlI	ACTAGT	1 cut(s)	566
AjnI	CCWGG	5 cut(s)	298, 1055, 1222, 1357, 2571
AjuI	GAANNNNNNNTTGG	2 cut(s)	1917, 1949
AleI	CACNNNNGTG	1 cut(s)	612
Alw21I	GWGCWC	2 cut(s)	1578, 2179
Alw26I	GTCTC	5 cut(s)	314, 557, 1214, 1884, 2135
Alw44I	GTGCAC	1 cut(s)	1574
AlwI	GGATC	7 cut(s)	212, 225, 467, 480, 1478, 2440, 2453
AlwNI	CAGNNNCTG	1 cut(s)	83
Aor51HI	AGCGCT	1 cut(s)	891
AoxI	GGCC	7 cut(s)	33, 433, 1216, 1458, 2191, 2339, 2569
ApaI	GGGCCC	1 cut(s)	2343
ApaLI	GTGCAC	1 cut(s)	1574
ApeKI	GCWGC	9 cut(s)	233, 650, 838, 985, 1313, 1730, 1789, 1844, 2483
ApoI	RAATTY	3 cut(s)	1010, 1078, 1157
AspLEI	GCGC	2 cut(s)	892, 1099
AspS9I	GGNCC	4 cut(s)	303, 1217, 2339, 2340
AsuC2I	CCSGG	4 cut(s)	29, 189, 1763, 2440
AsuHPI	GGTGA	4 cut(s)	30, 1732, 1877, 2636
AsuII	TTCGAA	1 cut(s)	1948
AvaII	GGWCC	1 cut(s)	303
AxyI	CCTNAGG	1 cut(s)	2331
BaeGI	GKGCMC	3 cut(s)	1578, 2224, 2343
BamHI	GGATCC	3 cut(s)	217, 472, 2445
BanI	GGYRCC	1 cut(s)	1349
BanII	GRGCYC	1 cut(s)	2343
BbrPI	CACGTG	1 cut(s)	1685
BbsI	GAAGAC	2 cut(s)	1682, 2510
Bbv12I	GWGCWC	2 cut(s)	1578, 2179
BbvI	GCAGC	9 cut(s)	245, 637, 850, 997, 1325, 1742, 1801, 1856, 2495
BccI	CCATC	5 cut(s)	454, 1360, 1628, 2330, 2516
BciT130I	CCWGG	5 cut(s)	300, 1057, 1224, 1359, 2573
BclI	TGATCA	1 cut(s)	2626
BcnI	CCSGG	4 cut(s)	29, 189, 1763, 2440
BcoDI	GTCTC	5 cut(s)	314, 557, 1214, 1884, 2135
BcuI	ACTAGT	1 cut(s)	566
BfaI	CTAG	9 cut(s)	312, 567, 701, 731, 1034, 1481, 1508, 1659, 1959
BfmI	CTRYAG	5 cut(s)	239, 1293, 1896, 2316, 2596
BfoI	RGCGCY	1 cut(s)	893
BfuAI	ACCTGC	2 cut(s)	1760, 1903
BlpI	GCTNAGC	1 cut(s)	974
BmcAI	AGTACT	1 cut(s)	690
Bme1390I	CCNGG	9 cut(s)	29, 189, 300, 1057, 1224, 1359, 1763, 2440, 2573
Bme18I	GGWCC	1 cut(s)	303
BmgT120I	GGNCC	4 cut(s)	303, 1217, 2339, 2340
BmiI	GGNNCC	7 cut(s)	219, 304, 474, 1351, 1781, 2341, 2447
BmrFI	CCNGG	9 cut(s)	29, 189, 300, 1057, 1224, 1359, 1763, 2440, 2573
BmrI	ACTGGG	3 cut(s)	872, 2337, 2430
BmsI	GCATC	4 cut(s)	782, 985, 1339, 1717
BmuI	ACTGGG	3 cut(s)	872, 2337, 2430
BoxI	GACNNNNGTC	1 cut(s)	2598
BpiI	GAAGAC	2 cut(s)	1682, 2510
BpmI	CTGGAG	3 cut(s)	581, 1245, 1769
Bpu1102I	GCTNAGC	1 cut(s)	974
Bpu14I	TTCGAA	1 cut(s)	1948
BpuMI	CCSGG	4 cut(s)	29, 189, 1763, 2440
Bsa29I	ATCGAT	2 cut(s)	1416, 2249
BsaAI	YACGTR	2 cut(s)	1025, 1685
BsaBI	GATNNNNATC	3 cut(s)	471, 755, 1116
BsaI	GGTCTC	1 cut(s)	2135
BsaJI	CCNNGG	6 cut(s)	27, 299, 1055, 1056, 1542, 2115
BsaXI	ACNNNNNCTCC	4 cut(s)	30, 60, 405, 435
Bse118I	RCCGGY	3 cut(s)	255, 272, 1855
Bse1I	ACTGG	5 cut(s)	867, 999, 1461, 2343, 2425
Bse21I	CCTNAGG	1 cut(s)	2331
Bse3DI	GCAATG	5 cut(s)	158, 164, 683, 1653, 2421
Bse8I	GATNNNNATC	3 cut(s)	471, 755, 1116
BseBI	CCWGG	5 cut(s)	300, 1057, 1224, 1359, 2573
BseCI	ATCGAT	2 cut(s)	1416, 2249
BseDI	CCNNGG	6 cut(s)	27, 299, 1055, 1056, 1542, 2115
BseGI	GGATG	8 cut(s)	298, 508, 1000, 1453, 2209, 2341, 2366, 2581
BseJI	GATNNNNATC	3 cut(s)	471, 755, 1116
BseMI	GCAATG	5 cut(s)	158, 164, 683, 1653, 2421
BseMII	CTCAG	6 cut(s)	75, 309, 617, 1192, 1899, 2162
BseNI	ACTGG	5 cut(s)	867, 999, 1461, 2343, 2425
BseRI	GAGGAG	3 cut(s)	1581, 2503, 2558
BseSI	GKGCMC	3 cut(s)	1578, 2224, 2343
BseXI	GCAGC	9 cut(s)	245, 637, 850, 997, 1325, 1742, 1801, 1856, 2495
BseYI	CCCAGC	1 cut(s)	1625
BshFI	GGCC	7 cut(s)	35, 435, 1218, 1460, 2193, 2341, 2571
BshNI	GGYRCC	1 cut(s)	1349
BshVI	ATCGAT	2 cut(s)	1416, 2249
BsiHKAI	GWGCWC	2 cut(s)	1578, 2179
BsiSI	CCGG	8 cut(s)	29, 188, 256, 273, 1457, 1762, 1856, 2439
BslFI	GGGAC	1 cut(s)	316
BsmAI	GTCTC	5 cut(s)	314, 557, 1214, 1884, 2135
BsmBI	CGTCTC	1 cut(s)	314
BsmFI	GGGAC	1 cut(s)	316
BsmI	GAATGC	2 cut(s)	333, 405
BsnI	GGCC	7 cut(s)	35, 435, 1218, 1460, 2193, 2341, 2571
Bso31I	GGTCTC	1 cut(s)	2135
Bsp119I	TTCGAA	1 cut(s)	1948
Bsp120I	GGGCCC	1 cut(s)	2339
Bsp1286I	GDGCHC	4 cut(s)	1578, 2179, 2224, 2343
Bsp1407I	TGTACA	2 cut(s)	1559, 2133
Bsp1720I	GCTNAGC	1 cut(s)	974
Bsp19I	CCATGG	1 cut(s)	2115
BspACI	CCGC	5 cut(s)	32, 170, 1810, 2460, 2496
BspANI	GGCC	7 cut(s)	35, 435, 1218, 1460, 2193, 2341, 2571
BspCNI	CTCAG	6 cut(s)	76, 310, 616, 1191, 1898, 2161
BspDI	ATCGAT	2 cut(s)	1416, 2249
BspHI	TCATGA	1 cut(s)	2208
BspLI	GGNNCC	7 cut(s)	219, 304, 474, 1351, 1781, 2341, 2447
BspMAI	CTGCAG	2 cut(s)	1900, 2320
BspMI	ACCTGC	2 cut(s)	1760, 1903
BspPI	GGATC	7 cut(s)	212, 225, 467, 480, 1478, 2440, 2453
BspQI	GCTCTTC	3 cut(s)	690, 963, 1740
BspT104I	TTCGAA	1 cut(s)	1948
BspT107I	GGYRCC	1 cut(s)	1349
BspTNI	GGTCTC	1 cut(s)	2135
BsrDI	GCAATG	5 cut(s)	158, 164, 683, 1653, 2421
BsrFI	RCCGGY	3 cut(s)	255, 272, 1855
BsrGI	TGTACA	2 cut(s)	1559, 2133
BsrI	ACTGG	5 cut(s)	867, 999, 1461, 2343, 2425
BssAI	RCCGGY	3 cut(s)	255, 272, 1855
BssECI	CCNNGG	6 cut(s)	27, 299, 1055, 1056, 1542, 2115
BssT1I	CCWWGG	2 cut(s)	1542, 2115
Bst2UI	CCWGG	5 cut(s)	300, 1057, 1224, 1359, 2573
Bst4CI	ACNGT	9 cut(s)	42, 183, 1070, 1274, 1387, 1878, 1993, 2239, 2600
Bst6I	CTCTTC	5 cut(s)	690, 789, 963, 1740, 1945
BstAUI	TGTACA	2 cut(s)	1559, 2133
BstBAI	YACGTR	2 cut(s)	1025, 1685
BstBI	TTCGAA	1 cut(s)	1948
BstC8I	GCNNGC	3 cut(s)	390, 1216, 1446
BstDEI	CTNAG	9 cut(s)	84, 318, 548, 603, 974, 1178, 1885, 2148, 2331
BstDSI	CCRYGG	1 cut(s)	2115
BstEII	GGTNACC	1 cut(s)	1720
BstENI	CCTNNNNNAGG	3 cut(s)	287, 1227, 1541
BstF5I	GGATG	8 cut(s)	298, 508, 1000, 1453, 2209, 2341, 2366, 2581
BstH2I	RGCGCY	1 cut(s)	893
BstHHI	GCGC	2 cut(s)	892, 1099
BstMAI	GTCTC	5 cut(s)	314, 557, 1214, 1884, 2135
BstMWI	GCNNNNNNNGC	5 cut(s)	169, 706, 1739, 2111, 2228
BstNI	CCWGG	5 cut(s)	300, 1057, 1224, 1359, 2573
BstNSI	RCATGY	5 cut(s)	202, 1265, 1448, 1695, 1931
BstPAI	GACNNNNGTC	1 cut(s)	2598
BstPI	GGTNACC	1 cut(s)	1720
BstSCI	CCNGG	9 cut(s)	27, 187, 298, 1055, 1222, 1357, 1761, 2438, 2571
BstSFI	CTRYAG	5 cut(s)	239, 1293, 1896, 2316, 2596
BstSLI	GKGCMC	3 cut(s)	1578, 2224, 2343
BstV1I	GCAGC	9 cut(s)	245, 637, 850, 997, 1325, 1742, 1801, 1856, 2495
BstV2I	GAAGAC	2 cut(s)	1682, 2510
BstX2I	RGATCY	4 cut(s)	217, 472, 1483, 2445
BstYI	RGATCY	4 cut(s)	217, 472, 1483, 2445
Bsu15I	ATCGAT	2 cut(s)	1416, 2249
Bsu36I	CCTNAGG	1 cut(s)	2331
BsuRI	GGCC	7 cut(s)	35, 435, 1218, 1460, 2193, 2341, 2571
BsuTUI	ATCGAT	2 cut(s)	1416, 2249
BtgI	CCRYGG	1 cut(s)	2115
BtgZI	GCGATG	1 cut(s)	1606
BtsCI	GGATG	8 cut(s)	298, 508, 1000, 1453, 2209, 2341, 2366, 2581
BtsIMutI	CAGTG	5 cut(s)	179, 1075, 1468, 2235, 2350
BveI	ACCTGC	2 cut(s)	1760, 1903
Cac8I	GCNNGC	3 cut(s)	390, 1216, 1446
CaiI	CAGNNNCTG	1 cut(s)	83
CciI	TCATGA	1 cut(s)	2208
CfoI	GCGC	2 cut(s)	892, 1099
Cfr10I	RCCGGY	3 cut(s)	255, 272, 1855
Cfr13I	GGNCC	4 cut(s)	303, 1217, 2339, 2340
ClaI	ATCGAT	2 cut(s)	1416, 2249
CseI	GACGC	2 cut(s)	806, 1319
Csp6I	GTAC	6 cut(s)	384, 689, 1370, 1560, 2134, 2355
CspCI	CAANNNNNGTGG	2 cut(s)	2134, 2169
CviQI	GTAC	6 cut(s)	384, 689, 1370, 1560, 2134, 2355
DdeI	CTNAG	9 cut(s)	84, 318, 548, 603, 974, 1178, 1885, 2148, 2331
DraI	TTTAAA	1 cut(s)	720
EaeI	YGGCCR	2 cut(s)	433, 1458
Eam1104I	CTCTTC	5 cut(s)	690, 789, 963, 1740, 1945
EarI	CTCTTC	5 cut(s)	690, 789, 963, 1740, 1945
EciI	GGCGGA	1 cut(s)	2511
Eco130I	CCWWGG	2 cut(s)	1542, 2115
Eco147I	AGGCCT	1 cut(s)	2571
Eco24I	GRGCYC	1 cut(s)	2343
Eco31I	GGTCTC	1 cut(s)	2135
Eco47I	GGWCC	1 cut(s)	303
Eco47III	AGCGCT	1 cut(s)	891
Eco57I	CTGAAG	3 cut(s)	156, 1155, 2505
Eco72I	CACGTG	1 cut(s)	1685
Eco81I	CCTNAGG	1 cut(s)	2331
Eco91I	GGTNACC	1 cut(s)	1720
EcoNI	CCTNNNNNAGG	3 cut(s)	287, 1227, 1541
EcoO65I	GGTNACC	1 cut(s)	1720
EcoRII	CCWGG	5 cut(s)	298, 1055, 1222, 1357, 2571
EcoT14I	CCWWGG	2 cut(s)	1542, 2115
EcoT38I	GRGCYC	1 cut(s)	2343
ErhI	CCWWGG	2 cut(s)	1542, 2115
Esp3I	CGTCTC	1 cut(s)	314
FalI	AAGNNNNNCTT	4 cut(s)	957, 989, 2163, 2195
FaqI	GGGAC	1 cut(s)	316
FauI	CCCGC	1 cut(s)	2453
FauNDI	CATATG	3 cut(s)	268, 493, 529
FbaI	TGATCA	1 cut(s)	2626
FokI	GGATG	8 cut(s)	305, 515, 1007, 1440, 2216, 2348, 2353, 2588
FriOI	GRGCYC	1 cut(s)	2343
FspBI	CTAG	9 cut(s)	312, 567, 701, 731, 1034, 1481, 1508, 1659, 1959
GlaI	GCGC	2 cut(s)	891, 1098
GsaI	CCCAGC	1 cut(s)	1629
GsuI	CTGGAG	3 cut(s)	581, 1245, 1769
HaeII	RGCGCY	1 cut(s)	893
HaeIII	GGCC	7 cut(s)	35, 435, 1218, 1460, 2193, 2341, 2571
HapII	CCGG	8 cut(s)	29, 188, 256, 273, 1457, 1762, 1856, 2439
HgaI	GACGC	2 cut(s)	806, 1319
HhaI	GCGC	2 cut(s)	892, 1099
Hin6I	GCGC	2 cut(s)	890, 1097
HinP1I	GCGC	2 cut(s)	890, 1097
HincII	GTYRAC	3 cut(s)	1305, 2011, 2401
HindII	GTYRAC	3 cut(s)	1305, 2011, 2401
HindIII	AAGCTT	1 cut(s)	2297
HinfI	GANTC	7 cut(s)	86, 766, 1582, 1803, 2012, 2077, 2291
HpaI	GTTAAC	1 cut(s)	1305
HpaII	CCGG	8 cut(s)	29, 188, 256, 273, 1457, 1762, 1856, 2439
HphI	GGTGA	4 cut(s)	30, 1732, 1877, 2636
Hpy166II	GTNNAC	5 cut(s)	1305, 1576, 2011, 2401, 2581
Hpy188I	TCNGA	8 cut(s)	50, 319, 811, 1181, 1192, 1413, 1888, 2604
Hpy8I	GTNNAC	5 cut(s)	1305, 1576, 2011, 2401, 2581
Hpy99I	CGWCG	2 cut(s)	822, 1338
HpyAV	CCTTC	9 cut(s)	30, 64, 208, 850, 1187, 1431, 2459, 2529, 2561
HpyCH4III	ACNGT	9 cut(s)	42, 183, 1070, 1274, 1387, 1878, 1993, 2239, 2600
HpyCH4IV	ACGT	4 cut(s)	715, 1024, 1344, 1684
HpyF10VI	GCNNNNNNNGC	5 cut(s)	169, 706, 1739, 2111, 2228
HpyF3I	CTNAG	9 cut(s)	84, 318, 548, 603, 974, 1178, 1885, 2148, 2331
HpySE526I	ACGT	4 cut(s)	715, 1024, 1344, 1684
HspAI	GCGC	2 cut(s)	890, 1097
Ksp22I	TGATCA	1 cut(s)	2626
KspAI	GTTAAC	1 cut(s)	1305
LguI	GCTCTTC	3 cut(s)	690, 963, 1740
LmnI	GCTCC	4 cut(s)	1474, 1841, 2174, 2545
Lsp1109I	GCAGC	9 cut(s)	245, 637, 850, 997, 1325, 1742, 1801, 1856, 2495
LweI	GCATC	4 cut(s)	782, 985, 1339, 1717
MaeI	CTAG	9 cut(s)	312, 567, 701, 731, 1034, 1481, 1508, 1659, 1959
MaeII	ACGT	4 cut(s)	715, 1024, 1344, 1684
MaeIII	GTNAC	5 cut(s)	478, 1018, 1720, 2185, 2428
MfeI	CAATTG	3 cut(s)	1254, 1530, 2454
MflI	RGATCY	4 cut(s)	217, 472, 1483, 2445
MhlI	GDGCHC	4 cut(s)	1578, 2179, 2224, 2343
MlyI	GAGTC	3 cut(s)	95, 2006, 2071
MmeI	TCCRAC	6 cut(s)	972, 1150, 1452, 1578, 1610, 2065
MslI	CAYNNNNRTG	1 cut(s)	612
MspA1I	CMGCKG	2 cut(s)	988, 1316
MspI	CCGG	8 cut(s)	29, 188, 256, 273, 1457, 1762, 1856, 2439
MspR9I	CCNGG	9 cut(s)	29, 189, 300, 1057, 1224, 1359, 1763, 2440, 2573
MunI	CAATTG	3 cut(s)	1254, 1530, 2454
Mva1269I	GAATGC	2 cut(s)	333, 405
MvaI	CCWGG	5 cut(s)	300, 1057, 1224, 1359, 2573
MwoI	GCNNNNNNNGC	5 cut(s)	169, 706, 1739, 2111, 2228
NciI	CCSGG	4 cut(s)	29, 189, 1763, 2440
NcoI	CCATGG	1 cut(s)	2115
NdeI	CATATG	3 cut(s)	268, 493, 529
NlaIV	GGNNCC	7 cut(s)	219, 304, 474, 1351, 1781, 2341, 2447
NmuCI	GTSAC	3 cut(s)	478, 1720, 2185
NspI	RCATGY	5 cut(s)	202, 1265, 1448, 1695, 1931
NspV	TTCGAA	1 cut(s)	1948
OliI	CACNNNNGTG	1 cut(s)	612
PaeI	GCATGC	1 cut(s)	1448
PagI	TCATGA	1 cut(s)	2208
PaqCI	CACCTGC	1 cut(s)	1760
PasI	CCCWGGG	1 cut(s)	1056
PceI	AGGCCT	1 cut(s)	2571
PciI	ACATGT	1 cut(s)	1927
PciSI	GCTCTTC	3 cut(s)	690, 963, 1740
PcsI	WCGNNNNNNNCGW	1 cut(s)	2628
PctI	GAATGC	2 cut(s)	333, 405
PfeI	GAWTC	4 cut(s)	766, 1582, 1803, 2291
PleI	GAGTC	3 cut(s)	94, 2006, 2071
PmaCI	CACGTG	1 cut(s)	1685
PmlI	CACGTG	1 cut(s)	1685
PpsI	GAGTC	3 cut(s)	94, 2006, 2071
Ppu21I	YACGTR	2 cut(s)	1025, 1685
PscI	ACATGT	1 cut(s)	1927
PshAI	GACNNNNGTC	1 cut(s)	2598
PsiI	TTATAA	1 cut(s)	515
Psp1406I	AACGTT	1 cut(s)	715
Psp6I	CCWGG	5 cut(s)	298, 1055, 1222, 1357, 2571
PspCI	CACGTG	1 cut(s)	1685
PspEI	GGTNACC	1 cut(s)	1720
PspFI	CCCAGC	1 cut(s)	1625
PspGI	CCWGG	5 cut(s)	298, 1055, 1222, 1357, 2571
PspN4I	GGNNCC	7 cut(s)	219, 304, 474, 1351, 1781, 2341, 2447
PspOMI	GGGCCC	1 cut(s)	2339
PspPI	GGNCC	4 cut(s)	303, 1217, 2339, 2340
PstI	CTGCAG	2 cut(s)	1900, 2320
PstNI	CAGNNNCTG	1 cut(s)	83
PsuI	RGATCY	4 cut(s)	217, 472, 1483, 2445
PvuII	CAGCTG	2 cut(s)	988, 1316
RsaI	GTAC	6 cut(s)	385, 690, 1371, 1561, 2135, 2356
RsaNI	GTAC	6 cut(s)	384, 689, 1370, 1560, 2134, 2355
RseI	CAYNNNNRTG	1 cut(s)	612
SapI	GCTCTTC	3 cut(s)	690, 963, 1740
Sau96I	GGNCC	4 cut(s)	303, 1217, 2339, 2340
SbfI	CCTGCAGG	1 cut(s)	2320
ScaI	AGTACT	1 cut(s)	690
SchI	GAGTC	3 cut(s)	95, 2006, 2071
ScrFI	CCNGG	9 cut(s)	29, 189, 300, 1057, 1224, 1359, 1763, 2440, 2573
SdaI	CCTGCAGG	1 cut(s)	2320
SduI	GDGCHC	4 cut(s)	1578, 2179, 2224, 2343
SfaNI	GCATC	4 cut(s)	782, 985, 1339, 1717
SfcI	CTRYAG	5 cut(s)	239, 1293, 1896, 2316, 2596
SfuI	TTCGAA	1 cut(s)	1948
SinI	GGWCC	1 cut(s)	303
SmiMI	CAYNNNNRTG	1 cut(s)	612
SpeI	ACTAGT	1 cut(s)	566
SphI	GCATGC	1 cut(s)	1448
Sse8387I	CCTGCAGG	1 cut(s)	2320
SseBI	AGGCCT	1 cut(s)	2571
SsiI	CCGC	5 cut(s)	32, 170, 1810, 2460, 2496
SspI	AATATT	1 cut(s)	1966
SspMI	CTAG	9 cut(s)	312, 567, 701, 731, 1034, 1481, 1508, 1659, 1959
StuI	AGGCCT	1 cut(s)	2571
StyD4I	CCNGG	9 cut(s)	27, 187, 298, 1055, 1222, 1357, 1761, 2438, 2571
StyI	CCWWGG	2 cut(s)	1542, 2115
TaaI	ACNGT	9 cut(s)	42, 183, 1070, 1274, 1387, 1878, 1993, 2239, 2600
TaiI	ACGT	4 cut(s)	718, 1027, 1347, 1687
TaqI	TCGA	6 cut(s)	1110, 1285, 1336, 1416, 1948, 2249
TaqII	GACCGA	2 cut(s)	320, 1739
TatI	WGTACW	4 cut(s)	688, 1559, 2133, 2354
TauI	GCSGC	2 cut(s)	35, 172
TfiI	GAWTC	4 cut(s)	766, 1582, 1803, 2291
TscAI	CASTG	5 cut(s)	186, 1075, 1468, 2242, 2350
TseFI	GTSAC	3 cut(s)	478, 1720, 2185
TseI	GCWGC	9 cut(s)	233, 650, 838, 985, 1313, 1730, 1789, 1844, 2483
Tsp45I	GTSAC	3 cut(s)	478, 1720, 2185
TspGWI	ACGGA	1 cut(s)	338
TspRI	CASTG	5 cut(s)	186, 1075, 1468, 2242, 2350
VneI	GTGCAC	1 cut(s)	1574
VpaK11BI	GGWCC	1 cut(s)	303
XagI	CCTNNNNNAGG	3 cut(s)	287, 1227, 1541
XapI	RAATTY	3 cut(s)	1010, 1078, 1157
XbaI	TCTAGA	3 cut(s)	1033, 1480, 1507
XceI	RCATGY	5 cut(s)	202, 1265, 1448, 1695, 1931
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1468
XspI	CTAG	9 cut(s)	312, 567, 701, 731, 1034, 1481, 1508, 1659, 1959
ZrmI	AGTACT	1 cut(s)	690

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.