ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1934 |
| AatII | GACGTC | 1 cut(s) | 1217 |
| Acc36I | ACCTGC | 2 cut(s) | 1934, 2095 |
| Acc65I | GGTACC | 1 cut(s) | 1882 |
| AccB1I | GGYRCC | 1 cut(s) | 1882 |
| AccB7I | CCANNNNNTGG | 4 cut(s) | 55, 422, 2672, 2684 |
| AciI | CCGC | 7 cut(s) | 72, 320, 383, 988, 1863, 2393, 2796 |
| AclI | AACGTT | 1 cut(s) | 883 |
| AcoI | YGGCCR | 3 cut(s) | 73, 124, 2577 |
| AcsI | RAATTY | 4 cut(s) | 1178, 1272, 1325, 2301 |
| AcuI | CTGAAG | 4 cut(s) | 240, 749, 920, 2259 |
| AcvI | CACGTG | 2 cut(s) | 1430, 1853 |
| AcyI | GRCGYC | 1 cut(s) | 1214 |
| AdeI | CACNNNGTG | 1 cut(s) | 2575 |
| AfaI | GTAC | 6 cut(s) | 547, 878, 1729, 1884, 2049, 2557 |
| AfeI | AGCGCT | 1 cut(s) | 2514 |
| AflII | CTTAAG | 1 cut(s) | 715 |
| AflIII | ACRYGT | 3 cut(s) | 873, 1190, 1850 |
| AgeI | ACCGGT | 1 cut(s) | 1885 |
| AhlI | ACTAGT | 1 cut(s) | 734 |
| AjiI | CACGTC | 1 cut(s) | 874 |
| AjnI | CCWGG | 5 cut(s) | 337, 448, 1223, 1390, 1525 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1521, 1553 |
| Alw26I | GTCTC | 5 cut(s) | 1034, 1382, 1824, 2058, 2471 |
| AlwNI | CAGNNNCTG | 5 cut(s) | 455, 479, 1159, 2105, 2258 |
| Ama87I | CYCGRG | 1 cut(s) | 2594 |
| Aor51HI | AGCGCT | 1 cut(s) | 2514 |
| ApaI | GGGCCC | 1 cut(s) | 62 |
| ApeKI | GCWGC | 4 cut(s) | 818, 1006, 1156, 1904 |
| ApoI | RAATTY | 4 cut(s) | 1178, 1272, 1325, 2301 |
| AsiGI | ACCGGT | 1 cut(s) | 1885 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1272, 2615 |
| Asp718I | GGTACC | 1 cut(s) | 1882 |
| AspLEI | GCGC | 1 cut(s) | 2515 |
| AspS9I | GGNCC | 7 cut(s) | 58, 59, 78, 135, 1385, 2658, 2675 |
| AsuC2I | CCSGG | 1 cut(s) | 152 |
| AsuHPI | GGTGA | 8 cut(s) | 112, 448, 794, 919, 1631, 2273, 2324, 2375 |
| AsuII | TTCGAA | 1 cut(s) | 610 |
| AsuNHI | GCTAGC | 1 cut(s) | 379 |
| AvaI | CYCGRG | 1 cut(s) | 2594 |
| BaeGI | GKGCMC | 1 cut(s) | 62 |
| BalI | TGGCCA | 1 cut(s) | 2579 |
| BamHI | GGATCC | 3 cut(s) | 367, 634, 2781 |
| BanI | GGYRCC | 1 cut(s) | 1882 |
| BanII | GRGCYC | 2 cut(s) | 49, 62 |
| BauI | CACGAG | 1 cut(s) | 28 |
| BbrPI | CACGTG | 2 cut(s) | 1430, 1853 |
| BbsI | GAAGAC | 3 cut(s) | 737, 1290, 1850 |
| BbvCI | CCTCAGC | 3 cut(s) | 228, 234, 240 |
| BbvI | GCAGC | 4 cut(s) | 805, 1018, 1143, 1916 |
| BccI | CCATC | 4 cut(s) | 616, 913, 1528, 2666 |
| BceAI | ACGGC | 4 cut(s) | 79, 196, 422, 960 |
| BciT130I | CCWGG | 5 cut(s) | 339, 450, 1225, 1392, 1527 |
| BciVI | GTATCC | 2 cut(s) | 578, 2275 |
| BclI | TGATCA | 1 cut(s) | 2697 |
| BcnI | CCSGG | 1 cut(s) | 152 |
| BcoDI | GTCTC | 5 cut(s) | 1034, 1382, 1824, 2058, 2471 |
| BcuI | ACTAGT | 1 cut(s) | 734 |
| BfmI | CTRYAG | 6 cut(s) | 35, 261, 1151, 2031, 2088, 2148 |
| BfoI | RGCGCY | 1 cut(s) | 2516 |
| BfrI | CTTAAG | 1 cut(s) | 715 |
| BfuAI | ACCTGC | 2 cut(s) | 1934, 2095 |
| BfuI | GTATCC | 2 cut(s) | 578, 2275 |
| BglII | AGATCT | 1 cut(s) | 1977 |
| BisI | GCNGC | 9 cut(s) | 73, 320, 384, 819, 988, 1007, 1157, 1864, 1905 |
| BlsI | GCNGC | 9 cut(s) | 74, 321, 385, 820, 989, 1008, 1158, 1865, 1906 |
| Bme1390I | CCNGG | 6 cut(s) | 152, 339, 450, 1225, 1392, 1527 |
| BmeT110I | CYCGRG | 1 cut(s) | 2594 |
| BmgBI | CACGTC | 1 cut(s) | 874 |
| BmgT120I | GGNCC | 7 cut(s) | 58, 59, 78, 135, 1385, 2658, 2675 |
| BmrFI | CCNGG | 6 cut(s) | 152, 339, 450, 1225, 1392, 1527 |
| BmrI | ACTGGG | 1 cut(s) | 1040 |
| BmsI | GCATC | 3 cut(s) | 1875, 2026, 2409 |
| BmtI | GCTAGC | 1 cut(s) | 383 |
| BmuI | ACTGGG | 1 cut(s) | 1040 |
| BpiI | GAAGAC | 3 cut(s) | 737, 1290, 1850 |
| BpmI | CTGGAG | 4 cut(s) | 444, 1181, 1413, 2250 |
| Bpu10I | CCTNAGC | 4 cut(s) | 228, 234, 240, 246 |
| Bpu14I | TTCGAA | 1 cut(s) | 610 |
| BpuMI | CCSGG | 1 cut(s) | 152 |
| Bsa29I | ATCGAT | 1 cut(s) | 529 |
| BsaAI | YACGTR | 3 cut(s) | 1193, 1430, 1853 |
| BsaBI | GATNNNNATC | 3 cut(s) | 633, 923, 1683 |
| BsaHI | GRCGYC | 1 cut(s) | 1214 |
| BsaI | GGTCTC | 1 cut(s) | 2471 |
| BsaJI | CCNNGG | 8 cut(s) | 210, 338, 449, 571, 797, 1223, 1224, 2451 |
| BsaWI | WCCGGW | 2 cut(s) | 1885, 2416 |
| Bse118I | RCCGGY | 3 cut(s) | 75, 122, 1885 |
| Bse1I | ACTGG | 8 cut(s) | 1035, 1167, 2233, 2263, 2327, 2381, 2530, 2779 |
| Bse3DI | GCAATG | 4 cut(s) | 314, 1821, 2715, 2757 |
| Bse8I | GATNNNNATC | 3 cut(s) | 633, 923, 1683 |
| BseBI | CCWGG | 5 cut(s) | 339, 450, 1225, 1392, 1527 |
| BseCI | ATCGAT | 1 cut(s) | 529 |
| BseDI | CCNNGG | 8 cut(s) | 210, 338, 449, 571, 797, 1223, 1224, 2451 |
| BseGI | GGATG | 4 cut(s) | 448, 676, 2677, 2702 |
| BseJI | GATNNNNATC | 3 cut(s) | 633, 923, 1683 |
| BseMI | GCAATG | 4 cut(s) | 314, 1821, 2715, 2757 |
| BseMII | CTCAG | 8 cut(s) | 213, 219, 225, 231, 785, 1360, 2309, 2498 |
| BseNI | ACTGG | 8 cut(s) | 1035, 1167, 2233, 2263, 2327, 2381, 2530, 2779 |
| BseRI | GAGGAG | 2 cut(s) | 16, 1749 |
| BseSI | GKGCMC | 1 cut(s) | 62 |
| BseX3I | CGGCCG | 2 cut(s) | 73, 124 |
| BseXI | GCAGC | 4 cut(s) | 805, 1018, 1143, 1916 |
| BseYI | CCCAGC | 2 cut(s) | 215, 1793 |
| Bsh1285I | CGRYCG | 2 cut(s) | 76, 127 |
| BshNI | GGYRCC | 1 cut(s) | 1882 |
| BshTI | ACCGGT | 1 cut(s) | 1885 |
| BshVI | ATCGAT | 1 cut(s) | 529 |
| BsiEI | CGRYCG | 2 cut(s) | 76, 127 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2594 |
| BsiSI | CCGG | 9 cut(s) | 42, 76, 123, 127, 151, 400, 1625, 1886, 2417 |
| BslFI | GGGAC | 3 cut(s) | 232, 466, 1509 |
| BsmAI | GTCTC | 5 cut(s) | 1034, 1382, 1824, 2058, 2471 |
| BsmFI | GGGAC | 3 cut(s) | 232, 466, 1509 |
| BsmI | GAATGC | 1 cut(s) | 2272 |
| Bso31I | GGTCTC | 1 cut(s) | 2471 |
| BsoBI | CYCGRG | 1 cut(s) | 2594 |
| Bsp119I | TTCGAA | 1 cut(s) | 610 |
| Bsp120I | GGGCCC | 1 cut(s) | 58 |
| Bsp1286I | GDGCHC | 2 cut(s) | 49, 62 |
| Bsp1407I | TGTACA | 2 cut(s) | 876, 1727 |
| Bsp19I | CCATGG | 1 cut(s) | 2451 |
| BspACI | CCGC | 7 cut(s) | 72, 320, 383, 988, 1863, 2393, 2796 |
| BspCNI | CTCAG | 8 cut(s) | 214, 220, 226, 232, 784, 1359, 2308, 2497 |
| BspDI | ATCGAT | 1 cut(s) | 529 |
| BspMAI | CTGCAG | 2 cut(s) | 2092, 2152 |
| BspMI | ACCTGC | 2 cut(s) | 1934, 2095 |
| BspOI | GCTAGC | 1 cut(s) | 383 |
| BspQI | GCTCTTC | 2 cut(s) | 858, 1131 |
| BspT104I | TTCGAA | 1 cut(s) | 610 |
| BspT107I | GGYRCC | 1 cut(s) | 1882 |
| BspTI | CTTAAG | 1 cut(s) | 715 |
| BspTNI | GGTCTC | 1 cut(s) | 2471 |
| BsrDI | GCAATG | 4 cut(s) | 314, 1821, 2715, 2757 |
| BsrFI | RCCGGY | 3 cut(s) | 75, 122, 1885 |
| BsrGI | TGTACA | 2 cut(s) | 876, 1727 |
| BsrI | ACTGG | 8 cut(s) | 1035, 1167, 2233, 2263, 2327, 2381, 2530, 2779 |
| BssAI | RCCGGY | 3 cut(s) | 75, 122, 1885 |
| BssECI | CCNNGG | 8 cut(s) | 210, 338, 449, 571, 797, 1223, 1224, 2451 |
| BssNI | GRCGYC | 1 cut(s) | 1214 |
| BssSI | CACGAG | 1 cut(s) | 28 |
| BssT1I | CCWWGG | 3 cut(s) | 210, 571, 2451 |
| Bst2BI | CACGAG | 1 cut(s) | 28 |
| Bst2UI | CCWGG | 5 cut(s) | 339, 450, 1225, 1392, 1527 |
| Bst4CI | ACNGT | 8 cut(s) | 53, 301, 646, 1238, 1985, 2019, 2052, 2575 |
| Bst6I | CTCTTC | 4 cut(s) | 22, 858, 957, 1131 |
| BstACI | GRCGYC | 1 cut(s) | 1214 |
| BstAFI | CTTAAG | 1 cut(s) | 715 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1064, 1913, 2096 |
| BstAUI | TGTACA | 2 cut(s) | 876, 1727 |
| BstBAI | YACGTR | 3 cut(s) | 1193, 1430, 1853 |
| BstBI | TTCGAA | 1 cut(s) | 610 |
| BstDEI | CTNAG | 9 cut(s) | 222, 228, 234, 240, 246, 771, 1346, 2295, 2484 |
| BstDSI | CCRYGG | 1 cut(s) | 2451 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1709 |
| BstF5I | GGATG | 4 cut(s) | 448, 676, 2677, 2702 |
| BstH2I | RGCGCY | 1 cut(s) | 2516 |
| BstHHI | GCGC | 1 cut(s) | 2515 |
| BstMAI | GTCTC | 5 cut(s) | 1034, 1382, 1824, 2058, 2471 |
| BstMCI | CGRYCG | 2 cut(s) | 76, 127 |
| BstNI | CCWGG | 5 cut(s) | 339, 450, 1225, 1392, 1527 |
| BstNSI | RCATGY | 3 cut(s) | 352, 1616, 1863 |
| BstSCI | CCNGG | 6 cut(s) | 150, 337, 448, 1223, 1390, 1525 |
| BstSFI | CTRYAG | 6 cut(s) | 35, 261, 1151, 2031, 2088, 2148 |
| BstSLI | GKGCMC | 1 cut(s) | 62 |
| BstV1I | GCAGC | 4 cut(s) | 805, 1018, 1143, 1916 |
| BstV2I | GAAGAC | 3 cut(s) | 737, 1290, 1850 |
| BstX2I | RGATCY | 4 cut(s) | 367, 634, 1977, 2781 |
| BstYI | RGATCY | 4 cut(s) | 367, 634, 1977, 2781 |
| BstZI | CGGCCG | 2 cut(s) | 73, 124 |
| Bsu15I | ATCGAT | 1 cut(s) | 529 |
| BsuI | GTATCC | 2 cut(s) | 578, 2275 |
| BsuTUI | ATCGAT | 1 cut(s) | 529 |
| BtgI | CCRYGG | 1 cut(s) | 2451 |
| BtgZI | GCGATG | 1 cut(s) | 1484 |
| BtrI | CACGTC | 1 cut(s) | 874 |
| BtsCI | GGATG | 4 cut(s) | 448, 676, 2677, 2702 |
| BtsI | GCAGTG | 1 cut(s) | 851 |
| BtsIMutI | CAGTG | 2 cut(s) | 851, 2571 |
| BveI | ACCTGC | 2 cut(s) | 1934, 2095 |
| CaiI | CAGNNNCTG | 5 cut(s) | 455, 479, 1159, 2105, 2258 |
| CfoI | GCGC | 1 cut(s) | 2515 |
| Cfr10I | RCCGGY | 3 cut(s) | 75, 122, 1885 |
| Cfr13I | GGNCC | 7 cut(s) | 58, 59, 78, 135, 1385, 2658, 2675 |
| ClaI | ATCGAT | 1 cut(s) | 529 |
| Csp6I | GTAC | 6 cut(s) | 546, 877, 1728, 1883, 2048, 2556 |
| CspAI | ACCGGT | 1 cut(s) | 1885 |
| CviQI | GTAC | 6 cut(s) | 546, 877, 1728, 1883, 2048, 2556 |
| DdeI | CTNAG | 9 cut(s) | 222, 228, 234, 240, 246, 771, 1346, 2295, 2484 |
| DraI | TTTAAA | 1 cut(s) | 888 |
| DraIII | CACNNNGTG | 1 cut(s) | 2575 |
| EaeI | YGGCCR | 3 cut(s) | 73, 124, 2577 |
| EagI | CGGCCG | 2 cut(s) | 73, 124 |
| Eam1104I | CTCTTC | 4 cut(s) | 22, 858, 957, 1131 |
| EarI | CTCTTC | 4 cut(s) | 22, 858, 957, 1131 |
| EclXI | CGGCCG | 2 cut(s) | 73, 124 |
| Eco130I | CCWWGG | 3 cut(s) | 210, 571, 2451 |
| Eco24I | GRGCYC | 2 cut(s) | 49, 62 |
| Eco31I | GGTCTC | 1 cut(s) | 2471 |
| Eco47III | AGCGCT | 1 cut(s) | 2514 |
| Eco52I | CGGCCG | 2 cut(s) | 73, 124 |
| Eco57I | CTGAAG | 4 cut(s) | 240, 749, 920, 2259 |
| Eco72I | CACGTG | 2 cut(s) | 1430, 1853 |
| Eco88I | CYCGRG | 1 cut(s) | 2594 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1709 |
| EcoO109I | RGGNCCY | 1 cut(s) | 59 |
| EcoRII | CCWGG | 5 cut(s) | 337, 448, 1223, 1390, 1525 |
| EcoT14I | CCWWGG | 3 cut(s) | 210, 571, 2451 |
| EcoT22I | ATGCAT | 1 cut(s) | 2274 |
| EcoT38I | GRGCYC | 2 cut(s) | 49, 62 |
| ErhI | CCWWGG | 3 cut(s) | 210, 571, 2451 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 573, 605, 1125, 1157, 2499, 2531 |
| FaqI | GGGAC | 3 cut(s) | 232, 466, 1509 |
| FauI | CCCGC | 1 cut(s) | 2789 |
| FauNDI | CATATG | 1 cut(s) | 418 |
| FbaI | TGATCA | 1 cut(s) | 2697 |
| Fnu4HI | GCNGC | 9 cut(s) | 73, 320, 384, 819, 988, 1007, 1157, 1864, 1905 |
| FokI | GGATG | 4 cut(s) | 455, 683, 2684, 2689 |
| FriOI | GRGCYC | 2 cut(s) | 49, 62 |
| FseI | GGCCGGCC | 1 cut(s) | 79 |
| Fsp4HI | GCNGC | 9 cut(s) | 73, 320, 384, 819, 988, 1007, 1157, 1864, 1905 |
| GlaI | GCGC | 1 cut(s) | 2514 |
| GluI | GCNGC | 9 cut(s) | 73, 320, 384, 819, 988, 1007, 1157, 1864, 1905 |
| GsaI | CCCAGC | 2 cut(s) | 219, 1797 |
| GsuI | CTGGAG | 4 cut(s) | 444, 1181, 1413, 2250 |
| HaeII | RGCGCY | 1 cut(s) | 2516 |
| HapII | CCGG | 9 cut(s) | 42, 76, 123, 127, 151, 400, 1625, 1886, 2417 |
| HhaI | GCGC | 1 cut(s) | 2515 |
| Hin1I | GRCGYC | 1 cut(s) | 1214 |
| Hin6I | GCGC | 1 cut(s) | 2513 |
| HinP1I | GCGC | 1 cut(s) | 2513 |
| HincII | GTYRAC | 2 cut(s) | 1212, 2531 |
| HindII | GTYRAC | 2 cut(s) | 1212, 2531 |
| HindIII | AAGCTT | 3 cut(s) | 8, 932, 2633 |
| HinfI | GANTC | 8 cut(s) | 130, 170, 1353, 1438, 2156, 2351, 2413, 2627 |
| HpaII | CCGG | 9 cut(s) | 42, 76, 123, 127, 151, 400, 1625, 1886, 2417 |
| HphI | GGTGA | 8 cut(s) | 112, 448, 794, 919, 1631, 2273, 2324, 2375 |
| Hpy166II | GTNNAC | 6 cut(s) | 270, 877, 1109, 1212, 2264, 2531 |
| Hpy8I | GTNNAC | 6 cut(s) | 270, 877, 1109, 1212, 2264, 2531 |
| Hpy99I | CGWCG | 1 cut(s) | 182 |
| HpyAV | CCTTC | 6 cut(s) | 358, 433, 1018, 2489, 2734, 2795 |
| HpyCH4III | ACNGT | 8 cut(s) | 53, 301, 646, 1238, 1985, 2019, 2052, 2575 |
| HpyCH4IV | ACGT | 7 cut(s) | 873, 883, 1185, 1192, 1214, 1429, 1852 |
| HpyF3I | CTNAG | 9 cut(s) | 222, 228, 234, 240, 246, 771, 1346, 2295, 2484 |
| HpySE526I | ACGT | 7 cut(s) | 873, 883, 1185, 1192, 1214, 1429, 1852 |
| Hsp92I | GRCGYC | 1 cut(s) | 1214 |
| HspAI | GCGC | 1 cut(s) | 2513 |
| KpnI | GGTACC | 1 cut(s) | 1886 |
| KroI | GCCGGC | 1 cut(s) | 75 |
| KroNI | GCCGGC | 1 cut(s) | 77 |
| Ksp22I | TGATCA | 1 cut(s) | 2697 |
| LguI | GCTCTTC | 2 cut(s) | 858, 1131 |
| LmnI | GCTCC | 9 cut(s) | 44, 188, 418, 425, 815, 1642, 1762, 2231, 2510 |
| Lsp1109I | GCAGC | 4 cut(s) | 805, 1018, 1143, 1916 |
| LweI | GCATC | 3 cut(s) | 1875, 2026, 2409 |
| MaeII | ACGT | 7 cut(s) | 873, 883, 1185, 1192, 1214, 1429, 1852 |
| MaeIII | GTNAC | 7 cut(s) | 96, 782, 1186, 1894, 2209, 2363, 2764 |
| MfeI | CAATTG | 2 cut(s) | 1422, 2790 |
| MflI | RGATCY | 4 cut(s) | 367, 634, 1977, 2781 |
| MhlI | GDGCHC | 2 cut(s) | 49, 62 |
| MlsI | TGGCCA | 1 cut(s) | 2579 |
| MluNI | TGGCCA | 1 cut(s) | 2579 |
| MlyI | GAGTC | 1 cut(s) | 2407 |
| MmeI | TCCRAC | 6 cut(s) | 183, 1318, 1444, 1537, 1620, 2401 |
| Mox20I | TGGCCA | 1 cut(s) | 2579 |
| Mph1103I | ATGCAT | 1 cut(s) | 2274 |
| MroNI | GCCGGC | 1 cut(s) | 75 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1272, 2615 |
| MscI | TGGCCA | 1 cut(s) | 2579 |
| MslI | CAYNNNNRTG | 5 cut(s) | 780, 1617, 2643, 2705, 2720 |
| Msp20I | TGGCCA | 1 cut(s) | 2579 |
| MspA1I | CMGCKG | 1 cut(s) | 1156 |
| MspCI | CTTAAG | 1 cut(s) | 715 |
| MspI | CCGG | 9 cut(s) | 42, 76, 123, 127, 151, 400, 1625, 1886, 2417 |
| MspR9I | CCNGG | 6 cut(s) | 152, 339, 450, 1225, 1392, 1527 |
| MunI | CAATTG | 2 cut(s) | 1422, 2790 |
| Mva1269I | GAATGC | 1 cut(s) | 2272 |
| MvaI | CCWGG | 5 cut(s) | 339, 450, 1225, 1392, 1527 |
| NaeI | GCCGGC | 1 cut(s) | 77 |
| NciI | CCSGG | 1 cut(s) | 152 |
| NcoI | CCATGG | 1 cut(s) | 2451 |
| NdeI | CATATG | 1 cut(s) | 418 |
| NgoMIV | GCCGGC | 1 cut(s) | 75 |
| NheI | GCTAGC | 1 cut(s) | 379 |
| NmeAIII | GCCGAG | 1 cut(s) | 1961 |
| NmuCI | GTSAC | 5 cut(s) | 782, 1186, 1894, 2209, 2363 |
| NsiI | ATGCAT | 1 cut(s) | 2274 |
| NspI | RCATGY | 3 cut(s) | 352, 1616, 1863 |
| NspV | TTCGAA | 1 cut(s) | 610 |
| PaeI | GCATGC | 1 cut(s) | 1863 |
| PaeR7I | CTCGAG | 1 cut(s) | 2594 |
| PaqCI | CACCTGC | 1 cut(s) | 1934 |
| PasI | CCCWGGG | 1 cut(s) | 1224 |
| PciSI | GCTCTTC | 2 cut(s) | 858, 1131 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1299 |
| PctI | GAATGC | 1 cut(s) | 2272 |
| PdiI | GCCGGC | 1 cut(s) | 77 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1272, 2615 |
| PfeI | GAWTC | 7 cut(s) | 130, 170, 1353, 1438, 2156, 2351, 2627 |
| PflMI | CCANNNNNTGG | 4 cut(s) | 55, 422, 2672, 2684 |
| PfoI | TCCNGGA | 1 cut(s) | 1390 |
| PinAI | ACCGGT | 1 cut(s) | 1885 |
| PkrI | GCNGC | 9 cut(s) | 74, 321, 385, 820, 989, 1008, 1158, 1865, 1906 |
| PleI | GAGTC | 1 cut(s) | 2407 |
| PmaCI | CACGTG | 2 cut(s) | 1430, 1853 |
| PmlI | CACGTG | 2 cut(s) | 1430, 1853 |
| PpsI | GAGTC | 1 cut(s) | 2407 |
| Ppu21I | YACGTR | 3 cut(s) | 1193, 1430, 1853 |
| Psp1406I | AACGTT | 1 cut(s) | 883 |
| Psp6I | CCWGG | 5 cut(s) | 337, 448, 1223, 1390, 1525 |
| PspCI | CACGTG | 2 cut(s) | 1430, 1853 |
| PspFI | CCCAGC | 2 cut(s) | 215, 1793 |
| PspGI | CCWGG | 5 cut(s) | 337, 448, 1223, 1390, 1525 |
| PspOMI | GGGCCC | 1 cut(s) | 58 |
| PspPI | GGNCC | 7 cut(s) | 58, 59, 78, 135, 1385, 2658, 2675 |
| PstI | CTGCAG | 2 cut(s) | 2092, 2152 |
| PstNI | CAGNNNCTG | 5 cut(s) | 455, 479, 1159, 2105, 2258 |
| PsuI | RGATCY | 4 cut(s) | 367, 634, 1977, 2781 |
| PvuII | CAGCTG | 1 cut(s) | 1156 |
| RigI | GGCCGGCC | 1 cut(s) | 79 |
| RsaI | GTAC | 6 cut(s) | 547, 878, 1729, 1884, 2049, 2557 |
| RsaNI | GTAC | 6 cut(s) | 546, 877, 1728, 1883, 2048, 2556 |
| RseI | CAYNNNNRTG | 5 cut(s) | 780, 1617, 2643, 2705, 2720 |
| SapI | GCTCTTC | 2 cut(s) | 858, 1131 |
| SatI | GCNGC | 9 cut(s) | 73, 320, 384, 819, 988, 1007, 1157, 1864, 1905 |
| Sau96I | GGNCC | 7 cut(s) | 58, 59, 78, 135, 1385, 2658, 2675 |
| SchI | GAGTC | 1 cut(s) | 2407 |
| ScrFI | CCNGG | 6 cut(s) | 152, 339, 450, 1225, 1392, 1527 |
| SduI | GDGCHC | 2 cut(s) | 49, 62 |
| SfaNI | GCATC | 3 cut(s) | 1875, 2026, 2409 |
| SfcI | CTRYAG | 6 cut(s) | 35, 261, 1151, 2031, 2088, 2148 |
| Sfr274I | CTCGAG | 1 cut(s) | 2594 |
| SfuI | TTCGAA | 1 cut(s) | 610 |
| SlaI | CTCGAG | 1 cut(s) | 2594 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 780, 1617, 2643, 2705, 2720 |
| SmlI | CTYRAG | 2 cut(s) | 715, 2594 |
| SmoI | CTYRAG | 2 cut(s) | 715, 2594 |
| SpeI | ACTAGT | 1 cut(s) | 734 |
| SphI | GCATGC | 1 cut(s) | 1863 |
| SsiI | CCGC | 7 cut(s) | 72, 320, 383, 988, 1863, 2393, 2796 |
| SspI | AATATT | 1 cut(s) | 2589 |
| StyD4I | CCNGG | 6 cut(s) | 150, 337, 448, 1223, 1390, 1525 |
| StyI | CCWWGG | 3 cut(s) | 210, 571, 2451 |
| TaaI | ACNGT | 8 cut(s) | 53, 301, 646, 1238, 1985, 2019, 2052, 2575 |
| TaiI | ACGT | 7 cut(s) | 876, 886, 1188, 1195, 1217, 1432, 1855 |
| TatI | WGTACW | 3 cut(s) | 876, 1727, 2047 |
| TauI | GCSGC | 5 cut(s) | 75, 322, 386, 990, 1866 |
| TfiI | GAWTC | 7 cut(s) | 130, 170, 1353, 1438, 2156, 2351, 2627 |
| TscAI | CASTG | 2 cut(s) | 851, 2578 |
| TseFI | GTSAC | 5 cut(s) | 782, 1186, 1894, 2209, 2363 |
| TseI | GCWGC | 4 cut(s) | 818, 1006, 1156, 1904 |
| Tsp45I | GTSAC | 5 cut(s) | 782, 1186, 1894, 2209, 2363 |
| TspGWI | ACGGA | 1 cut(s) | 1308 |
| TspRI | CASTG | 2 cut(s) | 851, 2578 |
| Van91I | CCANNNNNTGG | 4 cut(s) | 55, 422, 2672, 2684 |
| Vha464I | CTTAAG | 1 cut(s) | 715 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1709 |
| XapI | RAATTY | 4 cut(s) | 1178, 1272, 1325, 2301 |
| XbaI | TCTAGA | 1 cut(s) | 1675 |
| XceI | RCATGY | 3 cut(s) | 352, 1616, 1863 |
| XhoI | CTCGAG | 1 cut(s) | 2594 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1272, 2615 |
| ZraI | GACGTC | 1 cut(s) | 1215 |
| Zsp2I | ATGCAT | 1 cut(s) | 2274 |