Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0001800

Physical Location & Seq

Genomic Coordinates Forward (+)

55597623 .. 55606653

Gene Structure 9031 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030117

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2844 bp

ATGCTGAAACTCCTCTCTCCTTCTTCCCCGGCGGCCTCACCGTTTCTCCGAGCCGTTGGCTCTCTCACCGGCTTCACTCGCCGGAATCCTAACTTGGGCCGTCGTGCTTTTTTTTGCTCCGACGCTAAAAACGGCGATGGTGTGGTGGAAATCGAATTCAAGGGTGTAGGAAATGAAGCAGAGGCTGAGTCCAAGTCGTCATCATCATCAGCCATCATACCCACAAATCTTAATCCTGAAGATTATGTAAAGGTTTTAGCATTGCCATTGCCGCATAGACCACTGTTTCCGGGTTTCTACATGCCAATATACGTGAAGGATCCTAAACTATTAGTAGCTCTACAGGAAAGTAGAAACCGGCAAGTTCCATACGCCGGTGCTTTCCTTGTCAAGGATGAACCAGGGACCGACCCTAGTTCTGAGACGGAGAATAAGATTCCTGATTTGAAAGGGAAAGAGTTGTTTGATCGTCTGCATGAAGTTGGTACGCTTGCCCAGATTTCAAGCATTCAAGGAGATCAGGTAGTCCTAATCGGCCATAGGCGACTTCGAATTACAGAGATGGTTGATGAGGATCCCGTGACTGTAAAAGTTGATCATCTCAAGGATAAGCCATATGACAAGGATGATGATGTTATAAAGGCAACATCATATGAGGTTATATCAAATATAAGAGATGTTCTGGAGACTAGTGTTCTTTGGAGAGATCATGCTCAAACATATACTCAGCATATAAGTGATTTTAATTTTACAAGGATAGCAGATTTTGGGGCTGCTATATCTGGTGCAAACAAATTGCAATGCCAAAAAGTACTTGAAGAGCTAGATGTGCATGAACGTTTAAAGCTCACACTAGAATTAGTGAAGAAAGAGATAGAGATCAGTGAGATTCAGGCATCTCTTATGAAAAAAATGGAAGAGAAAATGCTTTCTGAACAGCGTCGCAAGTTGTTAAATGAGCAGCTTAAAGCAATAAAAAAGGAACTGGGATTAGAGGCTGATGATAGAACAGCGCTTTGTGAAAAGTTTAGGGAGAGGCTTGAACCAAATAGGGAAAAATGCCCACCTAATGTTTTGCAAGTCATAGAAGAAGAGCTAAGCAAAATGCAGCTGTTGGATGCCAGTTCAAGTGAATTTAATATAACACGTAATTATCTAGATTGGTTGATTTCACTTCCCTGGGGAAATTACAGTGATGAGAATTTTGATGTTCTTCGGGCGCAGAAAGTTCTCGATGAAGATCATTATGGATTAACTGAAGTAAAAGAAAGGATATTGGAATTTATTGCTGTTGGAAAACTCAGAGGAATGTCAGAAGGAAAAATCATCTGTCTCGCTGGTCCACCTGGAGTAGGAAAAACCAGCATTGGTCGTTCAATTGCACATGCCTTGAACCGTAATTTCTTTCGATTTTCTGTAGGAGGGTTAACTGATGCAGCTGAAATTAAGGGGCATCGTCGAACCTATGTTGGTGCTATGCCAGGAAAGATGGTACAATGCCTTAAAACTGTGGGAACAGCTAACCCTCTGGTTCTGATCGATGAGATTGACAAGTTGGGAAGGGGGCATGCTGGTCATCCGGCAAGTGCATTGTTGGAGCTTCTAGATCCAGAGCAAAATGCTAATTTTCTAGACCATTATCTTGATGTTCCGATTGACCTATCTAAGGTTCTGTTTGTTTGTACAGCAAATGTTGTGCACATGATTCCAACTCCTCTGTTGGATAGAATGGAGGTCGTTTCCATCGCTGGGTACATTACTGATGAGAAAATCCACATTGCTAGAGAGTATTTGGAGAAGACCACACGTGAAGCATGTGGCATTGAACCTGAACAGGTACTAATATATGTCATTTTGTATCTAATTTGTTTAATATATGCAGTAGTTTTTATTTATATGGTAAATGACATATTTCAGGTTGAGGTGACCGATGCAGCTCTTCTTGCACTCATTGAAAATTATTGCAGGGAAGCAGGTGTTAGGAACCTCCAGCAGCACATAGAAAGGATCTACCGCAAGATAGCTCTGCAACTTGTTAGACAAGGAACAGCAAATGAACCGGCAGTTGGTGATCAAATACAGTCTCTCTCAGATGAACCTGCAGTTGTTGAAGTTGCAGATAGTGACGAACATGTTGTTGGTTCAAAGGTTCGAAGAGATACTGGAAATATTTCCCAAAGTGAAGCAACAAAAATGGTTGAGAAAGTGTTGGTTGACTCATTAAACTTACCTGATTTTGTTGGGAAACCTGTTTTCCATGCTGAACACATCTACGAACAGACTCCAGTTGGAGTTGTTATGGGTCTTGCTTGGACTGCCATGGGTGGTTGCCCCTTGTATATAGAGACCACTCAGGTTGAGGAAGCTCACGGGAAAGGAGCACTTCAAGTCACAGGCCAACTTGGGGATGTCATGAGAGAAAGTGCCCATATTGCTCACACTGTTGCCAGATCGATATTGCTTGATAAAGAGCCAGATAACACTTTCTTTGCCGATTCCAAGCTTCATCTCCATATTCCTGCAGGCGCTATACCTAAGGATGGGCCCAGTGCTGGTTGTACTATGATTACATCCATGCTCTCCCTTGCCATGAAGAAGCCTGTCAACAAGGATTTAGCAATGACTGGGGAAGTAACACTAACCGGGAGGATCCTTCCAATTGGCGGGGTAAAGGAGAAAACAATAGCAGCAAGGAGGGGCGGAGTGAAGACCATCATATTCCCTTCAGCTAACAAGAGGGATTACGAGGAGCTTACCCCCAATGTAAAGGAAGGCCTGGATGTTCACTTTGTAGATGACTACAGTCAGATATTCAACTTGGCTTTCGGTGATCACGAAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004176 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0006508 GO:0006515 GO:0006807 GO:0006996 GO:0007005 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019538 GO:0030163 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042623 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0051603 GO:0070011 GO:0070013 GO:0071704 GO:0071840 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA LON_substr_bdg Lon_C

Protein Analysis

948

Amino Acids

105.22

Weight (kDa)

5.49

Isoelectric Point (pI)

44.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LON_substr_bdg	PF02190	85 - 290	8e-35	ATP-dependent protease La (LON) substrate-binding domain
AAA	PF00004	443 - 580	1.8e-23	ATPase family associated with various cellular activities (AAA)
AAA_5	PF07728	443 - 521	7.3e-07	AAA domain (dynein-related subfamily)
Lon_lid	PF22667	639 - 682	1.1e-15	Lon protease AAA+ ATPase lid domain
Lon_C	PF05362	738 - 942	3.1e-74	Lon protease (S16) C-terminal proteolytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001800)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05790 AT3G05790 AT5G26860
fragaria_vesca	FvH4_6g07420 FvH4_7g03300 FvH4_7g03300 FvH4_7g03300
malus_domestica	MD02G1284000.v1.1 MD04G1193200.v1.1 MD07G1050000.v1.1 MD12G1206100.v1.1
prunus_persica	Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.6G313300_v2.0.a1
pyrus_communis	pycom02g24200 pycom04g17100 pycom07g03580 pycom12g19400
rosa_chinensis	RchiOBHm_Chr1g0325611 RchiOBHm_Chr3g0457021
rosa_laevigata	RLG00000025251 RLG00000030117
rosa_multiflora	Rmu_sc0000488.1_g000001
rosa_roxburghii	Rroxscaffold_4G00324580 Rroxscaffold_6G00422260
rosa_rugosa	Rorug01G0053100 Rorug01G0053200 Rorug01G0053200 Rorug03G0018600 Rorug03G0018700
rosa_samantha	Rh1AG068800 Rh1CG069200 Rh1DG073900 Rh3AG078300 Rh3BG080200 Rh3CG080100 Rh3DG081900
rosa_wichuraiana	Rw1G005700 Rw3G006720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	638
AarI	CACCTGC	1 cut(s)	1964
Acc36I	ACCTGC	2 cut(s)	1964, 2107
AciI	CCGC	5 cut(s)	32, 272, 2014, 2664, 2700
AclI	AACGTT	1 cut(s)	838
AclWI	GGATC	8 cut(s)	314, 327, 569, 582, 1601, 2015, 2644, 2657
AcoI	YGGCCR	1 cut(s)	535
AcsI	RAATTY	4 cut(s)	155, 1133, 1201, 1280
AcuI	CTGAAG	3 cut(s)	258, 1278, 2709
AcvI	CACGTG	1 cut(s)	1808
AfaI	GTAC	7 cut(s)	487, 813, 1494, 1684, 1754, 1839, 2560
AfeI	AGCGCT	1 cut(s)	1014
AflIII	ACRYGT	3 cut(s)	1145, 1805, 2131
AhlI	ACTAGT	1 cut(s)	689
AjnI	CCWGG	5 cut(s)	400, 1178, 1345, 1480, 2775
AjuI	GAANNNNNNNTTGG	6 cut(s)	77, 109, 2049, 2081, 2121, 2153
Alw21I	GWGCWC	2 cut(s)	1701, 2383
Alw26I	GTCTC	5 cut(s)	416, 680, 1337, 2088, 2339
Alw44I	GTGCAC	1 cut(s)	1697
AlwI	GGATC	8 cut(s)	314, 327, 569, 582, 1601, 2015, 2644, 2657
AlwNI	CAGNNNCTG	1 cut(s)	185
Aor51HI	AGCGCT	1 cut(s)	1014
AoxI	GGCC	6 cut(s)	33, 97, 535, 2395, 2543, 2773
ApaI	GGGCCC	1 cut(s)	2547
ApaLI	GTGCAC	1 cut(s)	1697
ApeKI	GCWGC	7 cut(s)	773, 961, 1108, 1436, 1934, 1993, 2687
ApoI	RAATTY	4 cut(s)	155, 1133, 1201, 1280
Asp700I	GAANNNNTTC	1 cut(s)	2170
AspLEI	GCGC	3 cut(s)	1015, 1222, 2528
AspS9I	GGNCC	5 cut(s)	97, 405, 1340, 2543, 2544
AsuC2I	CCSGG	3 cut(s)	29, 291, 2644
AsuHPI	GGTGA	5 cut(s)	30, 58, 1936, 2081, 2840
AsuII	TTCGAA	2 cut(s)	550, 2152
AvaII	GGWCC	2 cut(s)	405, 1340
AxyI	CCTNAGG	1 cut(s)	2535
BaeGI	GKGCMC	3 cut(s)	1701, 2428, 2547
BamHI	GGATCC	3 cut(s)	319, 574, 2649
BanII	GRGCYC	1 cut(s)	2547
BbrPI	CACGTG	1 cut(s)	1808
BbsI	GAAGAC	2 cut(s)	1805, 2714
Bbv12I	GWGCWC	2 cut(s)	1701, 2383
BbvI	GCAGC	7 cut(s)	760, 973, 1120, 1448, 1946, 2005, 2699
BccI	CCATC	7 cut(s)	131, 221, 556, 1483, 1751, 2534, 2720
BceAI	ACGGC	3 cut(s)	38, 84, 148
BciT130I	CCWGG	5 cut(s)	402, 1180, 1347, 1482, 2777
BclI	TGATCA	3 cut(s)	595, 2071, 2830
BcnI	CCSGG	3 cut(s)	29, 291, 2644
BcoDI	GTCTC	5 cut(s)	416, 680, 1337, 2088, 2339
BcuI	ACTAGT	1 cut(s)	689
BfaI	CTAG	8 cut(s)	414, 690, 824, 854, 1157, 1604, 1631, 1782
BfmI	CTRYAG	5 cut(s)	341, 1416, 2100, 2520, 2800
BfoI	RGCGCY	2 cut(s)	1016, 2529
BfuAI	ACCTGC	2 cut(s)	1964, 2107
BisI	GCNGC	9 cut(s)	33, 272, 774, 962, 1109, 1437, 1935, 1994, 2688
BlpI	GCTNAGC	1 cut(s)	1097
BlsI	GCNGC	9 cut(s)	34, 273, 775, 963, 1110, 1438, 1936, 1995, 2689
BmcAI	AGTACT	1 cut(s)	813
Bme1390I	CCNGG	8 cut(s)	29, 291, 402, 1180, 1347, 1482, 2644, 2777
Bme18I	GGWCC	2 cut(s)	405, 1340
BmgT120I	GGNCC	5 cut(s)	97, 405, 1340, 2543, 2544
BmiI	GGNNCC	6 cut(s)	321, 406, 576, 1985, 2545, 2651
BmrFI	CCNGG	8 cut(s)	29, 291, 402, 1180, 1347, 1482, 2644, 2777
BmrI	ACTGGG	3 cut(s)	995, 2541, 2634
BmsI	GCATC	5 cut(s)	905, 1108, 1423, 1462, 1921
BmuI	ACTGGG	3 cut(s)	995, 2541, 2634
BoxI	GACNNNNGTC	1 cut(s)	2802
BpiI	GAAGAC	2 cut(s)	1805, 2714
BpmI	CTGGAG	4 cut(s)	704, 1368, 1973, 2268
Bpu1102I	GCTNAGC	1 cut(s)	1097
Bpu14I	TTCGAA	2 cut(s)	550, 2152
BpuEI	CTTGAG	1 cut(s)	587
BpuMI	CCSGG	3 cut(s)	29, 291, 2644
Bsa29I	ATCGAT	2 cut(s)	1539, 2453
BsaAI	YACGTR	3 cut(s)	313, 1148, 1808
BsaBI	GATNNNNATC	3 cut(s)	573, 878, 1239
BsaI	GGTCTC	1 cut(s)	2339
BsaJI	CCNNGG	5 cut(s)	27, 401, 1178, 1179, 2319
BsaXI	ACNNNNNCTCC	4 cut(s)	30, 60, 507, 537
Bse118I	RCCGGY	4 cut(s)	68, 357, 374, 2059
Bse1I	ACTGG	6 cut(s)	990, 1122, 2167, 2285, 2547, 2629
Bse21I	CCTNAGG	1 cut(s)	2535
Bse3DI	GCAATG	5 cut(s)	260, 266, 806, 1776, 2625
Bse8I	GATNNNNATC	3 cut(s)	573, 878, 1239
BseBI	CCWGG	5 cut(s)	402, 1180, 1347, 1482, 2777
BseCI	ATCGAT	2 cut(s)	1539, 2453
BseDI	CCNNGG	5 cut(s)	27, 401, 1178, 1179, 2319
BseGI	GGATG	8 cut(s)	400, 631, 1123, 1576, 2413, 2545, 2570, 2785
BseJI	GATNNNNATC	3 cut(s)	573, 878, 1239
BseMI	GCAATG	5 cut(s)	260, 266, 806, 1776, 2625
BseMII	CTCAG	6 cut(s)	177, 411, 740, 1315, 2103, 2366
BseNI	ACTGG	6 cut(s)	990, 1122, 2167, 2285, 2547, 2629
BseRI	GAGGAG	2 cut(s)	1704, 2762
BseSI	GKGCMC	3 cut(s)	1701, 2428, 2547
BseXI	GCAGC	7 cut(s)	760, 973, 1120, 1448, 1946, 2005, 2699
BseYI	CCCAGC	1 cut(s)	1748
BshFI	GGCC	6 cut(s)	35, 99, 537, 2397, 2545, 2775
BshVI	ATCGAT	2 cut(s)	1539, 2453
BsiHKAI	GWGCWC	2 cut(s)	1701, 2383
BsiSI	CCGG	9 cut(s)	29, 69, 82, 290, 358, 375, 1580, 2060, 2643
BslFI	GGGAC	1 cut(s)	418
BsmAI	GTCTC	5 cut(s)	416, 680, 1337, 2088, 2339
BsmBI	CGTCTC	1 cut(s)	416
BsmFI	GGGAC	1 cut(s)	418
BsmI	GAATGC	1 cut(s)	507
BsnI	GGCC	6 cut(s)	35, 99, 537, 2397, 2545, 2775
Bso31I	GGTCTC	1 cut(s)	2339
Bsp119I	TTCGAA	2 cut(s)	550, 2152
Bsp120I	GGGCCC	1 cut(s)	2543
Bsp1286I	GDGCHC	4 cut(s)	1701, 2383, 2428, 2547
Bsp1407I	TGTACA	1 cut(s)	1682
Bsp1720I	GCTNAGC	1 cut(s)	1097
Bsp19I	CCATGG	1 cut(s)	2319
BspACI	CCGC	5 cut(s)	32, 272, 2014, 2664, 2700
BspANI	GGCC	6 cut(s)	35, 99, 537, 2397, 2545, 2775
BspCNI	CTCAG	6 cut(s)	178, 412, 739, 1314, 2102, 2365
BspDI	ATCGAT	2 cut(s)	1539, 2453
BspHI	TCATGA	1 cut(s)	2412
BspLI	GGNNCC	6 cut(s)	321, 406, 576, 1985, 2545, 2651
BspMAI	CTGCAG	2 cut(s)	2104, 2524
BspMI	ACCTGC	2 cut(s)	1964, 2107
BspPI	GGATC	8 cut(s)	314, 327, 569, 582, 1601, 2015, 2644, 2657
BspQI	GCTCTTC	3 cut(s)	813, 1086, 1944
BspT104I	TTCGAA	2 cut(s)	550, 2152
BspTNI	GGTCTC	1 cut(s)	2339
BsrDI	GCAATG	5 cut(s)	260, 266, 806, 1776, 2625
BsrFI	RCCGGY	4 cut(s)	68, 357, 374, 2059
BsrGI	TGTACA	1 cut(s)	1682
BsrI	ACTGG	6 cut(s)	990, 1122, 2167, 2285, 2547, 2629
BssAI	RCCGGY	4 cut(s)	68, 357, 374, 2059
BssECI	CCNNGG	5 cut(s)	27, 401, 1178, 1179, 2319
BssT1I	CCWWGG	1 cut(s)	2319
Bst2UI	CCWGG	5 cut(s)	402, 1180, 1347, 1482, 2777
Bst4CI	ACNGT	9 cut(s)	42, 285, 586, 1193, 1397, 1510, 2082, 2443, 2804
Bst6I	CTCTTC	5 cut(s)	813, 912, 1086, 1944, 2149
BstAUI	TGTACA	1 cut(s)	1682
BstBAI	YACGTR	3 cut(s)	313, 1148, 1808
BstBI	TTCGAA	2 cut(s)	550, 2152
BstC8I	GCNNGC	3 cut(s)	492, 1569, 2524
BstDEI	CTNAG	9 cut(s)	186, 420, 726, 1097, 1301, 1665, 2089, 2352, 2535
BstDSI	CCRYGG	1 cut(s)	2319
BstEII	GGTNACC	1 cut(s)	1924
BstENI	CCTNNNNNAGG	3 cut(s)	389, 1350, 1664
BstF5I	GGATG	8 cut(s)	400, 631, 1123, 1576, 2413, 2545, 2570, 2785
BstH2I	RGCGCY	2 cut(s)	1016, 2529
BstHHI	GCGC	3 cut(s)	1015, 1222, 2528
BstMAI	GTCTC	5 cut(s)	416, 680, 1337, 2088, 2339
BstMWI	GCNNNNNNNGC	6 cut(s)	78, 271, 829, 1943, 2315, 2432
BstNI	CCWGG	5 cut(s)	402, 1180, 1347, 1482, 2777
BstNSI	RCATGY	5 cut(s)	304, 1388, 1571, 1818, 2135
BstPAI	GACNNNNGTC	1 cut(s)	2802
BstPI	GGTNACC	1 cut(s)	1924
BstSCI	CCNGG	8 cut(s)	27, 289, 400, 1178, 1345, 1480, 2642, 2775
BstSFI	CTRYAG	5 cut(s)	341, 1416, 2100, 2520, 2800
BstSLI	GKGCMC	3 cut(s)	1701, 2428, 2547
BstV1I	GCAGC	7 cut(s)	760, 973, 1120, 1448, 1946, 2005, 2699
BstV2I	GAAGAC	2 cut(s)	1805, 2714
BstX2I	RGATCY	5 cut(s)	319, 574, 1606, 2007, 2649
BstYI	RGATCY	5 cut(s)	319, 574, 1606, 2007, 2649
Bsu15I	ATCGAT	2 cut(s)	1539, 2453
Bsu36I	CCTNAGG	1 cut(s)	2535
BsuRI	GGCC	6 cut(s)	35, 99, 537, 2397, 2545, 2775
BsuTUI	ATCGAT	2 cut(s)	1539, 2453
BtgI	CCRYGG	1 cut(s)	2319
BtgZI	GCGATG	2 cut(s)	150, 1729
BtsCI	GGATG	8 cut(s)	400, 631, 1123, 1576, 2413, 2545, 2570, 2785
BtsIMutI	CAGTG	5 cut(s)	281, 889, 1198, 2439, 2554
BveI	ACCTGC	2 cut(s)	1964, 2107
Cac8I	GCNNGC	3 cut(s)	492, 1569, 2524
CaiI	CAGNNNCTG	1 cut(s)	185
CciI	TCATGA	1 cut(s)	2412
CfoI	GCGC	3 cut(s)	1015, 1222, 2528
Cfr10I	RCCGGY	4 cut(s)	68, 357, 374, 2059
Cfr13I	GGNCC	5 cut(s)	97, 405, 1340, 2543, 2544
ClaI	ATCGAT	2 cut(s)	1539, 2453
CseI	GACGC	2 cut(s)	131, 929
Csp6I	GTAC	7 cut(s)	486, 812, 1493, 1683, 1753, 1838, 2559
CspCI	CAANNNNNGTGG	2 cut(s)	2338, 2373
CviQI	GTAC	7 cut(s)	486, 812, 1493, 1683, 1753, 1838, 2559
DdeI	CTNAG	9 cut(s)	186, 420, 726, 1097, 1301, 1665, 2089, 2352, 2535
DraI	TTTAAA	1 cut(s)	843
EaeI	YGGCCR	1 cut(s)	535
Eam1104I	CTCTTC	5 cut(s)	813, 912, 1086, 1944, 2149
EarI	CTCTTC	5 cut(s)	813, 912, 1086, 1944, 2149
EciI	GGCGGA	1 cut(s)	2715
Eco130I	CCWWGG	1 cut(s)	2319
Eco147I	AGGCCT	1 cut(s)	2775
Eco24I	GRGCYC	1 cut(s)	2547
Eco31I	GGTCTC	1 cut(s)	2339
Eco47I	GGWCC	2 cut(s)	405, 1340
Eco47III	AGCGCT	1 cut(s)	1014
Eco57I	CTGAAG	3 cut(s)	258, 1278, 2709
Eco72I	CACGTG	1 cut(s)	1808
Eco81I	CCTNAGG	1 cut(s)	2535
Eco91I	GGTNACC	1 cut(s)	1924
EcoNI	CCTNNNNNAGG	3 cut(s)	389, 1350, 1664
EcoO65I	GGTNACC	1 cut(s)	1924
EcoRI	GAATTC	1 cut(s)	155
EcoRII	CCWGG	5 cut(s)	400, 1178, 1345, 1480, 2775
EcoT14I	CCWWGG	1 cut(s)	2319
EcoT38I	GRGCYC	1 cut(s)	2547
ErhI	CCWWGG	1 cut(s)	2319
Esp3I	CGTCTC	1 cut(s)	416
FalI	AAGNNNNNCTT	2 cut(s)	2367, 2399
FaqI	GGGAC	1 cut(s)	418
FauI	CCCGC	1 cut(s)	2657
FauNDI	CATATG	2 cut(s)	616, 652
FbaI	TGATCA	3 cut(s)	595, 2071, 2830
Fnu4HI	GCNGC	9 cut(s)	33, 272, 774, 962, 1109, 1437, 1935, 1994, 2688
FokI	GGATG	8 cut(s)	407, 638, 1130, 1563, 2420, 2552, 2557, 2792
FriOI	GRGCYC	1 cut(s)	2547
Fsp4HI	GCNGC	9 cut(s)	33, 272, 774, 962, 1109, 1437, 1935, 1994, 2688
FspBI	CTAG	8 cut(s)	414, 690, 824, 854, 1157, 1604, 1631, 1782
GlaI	GCGC	3 cut(s)	1014, 1221, 2527
GluI	GCNGC	9 cut(s)	33, 272, 774, 962, 1109, 1437, 1935, 1994, 2688
GsaI	CCCAGC	1 cut(s)	1752
GsuI	CTGGAG	4 cut(s)	704, 1368, 1973, 2268
HaeII	RGCGCY	2 cut(s)	1016, 2529
HaeIII	GGCC	6 cut(s)	35, 99, 537, 2397, 2545, 2775
HapII	CCGG	9 cut(s)	29, 69, 82, 290, 358, 375, 1580, 2060, 2643
HgaI	GACGC	2 cut(s)	131, 929
HhaI	GCGC	3 cut(s)	1015, 1222, 2528
Hin6I	GCGC	3 cut(s)	1013, 1220, 2526
HinP1I	GCGC	3 cut(s)	1013, 1220, 2526
HincII	GTYRAC	3 cut(s)	1428, 2215, 2605
HindII	GTYRAC	3 cut(s)	1428, 2215, 2605
HindIII	AAGCTT	1 cut(s)	2501
HinfI	GANTC	8 cut(s)	85, 188, 436, 889, 1705, 2216, 2281, 2495
HpaI	GTTAAC	1 cut(s)	1428
HpaII	CCGG	9 cut(s)	29, 69, 82, 290, 358, 375, 1580, 2060, 2643
HphI	GGTGA	5 cut(s)	30, 58, 1936, 2081, 2840
Hpy166II	GTNNAC	6 cut(s)	1343, 1428, 1699, 2215, 2605, 2785
Hpy8I	GTNNAC	6 cut(s)	1343, 1428, 1699, 2215, 2605, 2785
Hpy99I	CGWCG	4 cut(s)	105, 125, 945, 1461
HpyAV	CCTTC	7 cut(s)	30, 310, 1310, 1554, 2663, 2733, 2765
HpyCH4III	ACNGT	9 cut(s)	42, 285, 586, 1193, 1397, 1510, 2082, 2443, 2804
HpyCH4IV	ACGT	4 cut(s)	312, 838, 1147, 1807
HpyF10VI	GCNNNNNNNGC	6 cut(s)	78, 271, 829, 1943, 2315, 2432
HpyF3I	CTNAG	9 cut(s)	186, 420, 726, 1097, 1301, 1665, 2089, 2352, 2535
HpySE526I	ACGT	4 cut(s)	312, 838, 1147, 1807
HspAI	GCGC	3 cut(s)	1013, 1220, 2526
Ksp22I	TGATCA	3 cut(s)	595, 2071, 2830
KspAI	GTTAAC	1 cut(s)	1428
LguI	GCTCTTC	3 cut(s)	813, 1086, 1944
LmnI	GCTCC	4 cut(s)	122, 1597, 2378, 2749
Lsp1109I	GCAGC	7 cut(s)	760, 973, 1120, 1448, 1946, 2005, 2699
LweI	GCATC	5 cut(s)	905, 1108, 1423, 1462, 1921
MaeI	CTAG	8 cut(s)	414, 690, 824, 854, 1157, 1604, 1631, 1782
MaeII	ACGT	4 cut(s)	312, 838, 1147, 1807
MaeIII	GTNAC	5 cut(s)	580, 1924, 2123, 2389, 2632
MfeI	CAATTG	2 cut(s)	1377, 2658
MflI	RGATCY	5 cut(s)	319, 574, 1606, 2007, 2649
MhlI	GDGCHC	4 cut(s)	1701, 2383, 2428, 2547
MlyI	GAGTC	3 cut(s)	197, 2210, 2275
MmeI	TCCRAC	7 cut(s)	144, 1095, 1273, 1575, 1701, 1733, 2269
MroXI	GAANNNNTTC	1 cut(s)	2170
MslI	CAYNNNNRTG	1 cut(s)	735
MspA1I	CMGCKG	2 cut(s)	1111, 1439
MspI	CCGG	9 cut(s)	29, 69, 82, 290, 358, 375, 1580, 2060, 2643
MspR9I	CCNGG	8 cut(s)	29, 291, 402, 1180, 1347, 1482, 2644, 2777
MunI	CAATTG	2 cut(s)	1377, 2658
Mva1269I	GAATGC	1 cut(s)	507
MvaI	CCWGG	5 cut(s)	402, 1180, 1347, 1482, 2777
MwoI	GCNNNNNNNGC	6 cut(s)	78, 271, 829, 1943, 2315, 2432
NciI	CCSGG	3 cut(s)	29, 291, 2644
NcoI	CCATGG	1 cut(s)	2319
NdeI	CATATG	2 cut(s)	616, 652
NlaIV	GGNNCC	6 cut(s)	321, 406, 576, 1985, 2545, 2651
NmuCI	GTSAC	4 cut(s)	580, 1924, 2123, 2389
NspI	RCATGY	5 cut(s)	304, 1388, 1571, 1818, 2135
NspV	TTCGAA	2 cut(s)	550, 2152
PaeI	GCATGC	1 cut(s)	1571
PagI	TCATGA	1 cut(s)	2412
PaqCI	CACCTGC	1 cut(s)	1964
PasI	CCCWGGG	1 cut(s)	1179
PceI	AGGCCT	1 cut(s)	2775
PciI	ACATGT	1 cut(s)	2131
PciSI	GCTCTTC	3 cut(s)	813, 1086, 1944
PcsI	WCGNNNNNNNCGW	1 cut(s)	2832
PctI	GAATGC	1 cut(s)	507
PdmI	GAANNNNTTC	1 cut(s)	2170
PfeI	GAWTC	5 cut(s)	85, 436, 889, 1705, 2495
PkrI	GCNGC	9 cut(s)	34, 273, 775, 963, 1110, 1438, 1936, 1995, 2689
PleI	GAGTC	3 cut(s)	196, 2210, 2275
PmaCI	CACGTG	1 cut(s)	1808
PmlI	CACGTG	1 cut(s)	1808
PpsI	GAGTC	3 cut(s)	196, 2210, 2275
Ppu21I	YACGTR	3 cut(s)	313, 1148, 1808
PscI	ACATGT	1 cut(s)	2131
PshAI	GACNNNNGTC	1 cut(s)	2802
PsiI	TTATAA	1 cut(s)	638
Psp1406I	AACGTT	1 cut(s)	838
Psp6I	CCWGG	5 cut(s)	400, 1178, 1345, 1480, 2775
PspCI	CACGTG	1 cut(s)	1808
PspEI	GGTNACC	1 cut(s)	1924
PspFI	CCCAGC	1 cut(s)	1748
PspGI	CCWGG	5 cut(s)	400, 1178, 1345, 1480, 2775
PspN4I	GGNNCC	6 cut(s)	321, 406, 576, 1985, 2545, 2651
PspOMI	GGGCCC	1 cut(s)	2543
PspPI	GGNCC	5 cut(s)	97, 405, 1340, 2543, 2544
PstI	CTGCAG	2 cut(s)	2104, 2524
PstNI	CAGNNNCTG	1 cut(s)	185
PsuI	RGATCY	5 cut(s)	319, 574, 1606, 2007, 2649
PvuII	CAGCTG	2 cut(s)	1111, 1439
RsaI	GTAC	7 cut(s)	487, 813, 1494, 1684, 1754, 1839, 2560
RsaNI	GTAC	7 cut(s)	486, 812, 1493, 1683, 1753, 1838, 2559
RseI	CAYNNNNRTG	1 cut(s)	735
SapI	GCTCTTC	3 cut(s)	813, 1086, 1944
SatI	GCNGC	9 cut(s)	33, 272, 774, 962, 1109, 1437, 1935, 1994, 2688
Sau96I	GGNCC	5 cut(s)	97, 405, 1340, 2543, 2544
SbfI	CCTGCAGG	1 cut(s)	2524
ScaI	AGTACT	1 cut(s)	813
SchI	GAGTC	3 cut(s)	197, 2210, 2275
ScrFI	CCNGG	8 cut(s)	29, 291, 402, 1180, 1347, 1482, 2644, 2777
SdaI	CCTGCAGG	1 cut(s)	2524
SduI	GDGCHC	4 cut(s)	1701, 2383, 2428, 2547
SfaNI	GCATC	5 cut(s)	905, 1108, 1423, 1462, 1921
SfcI	CTRYAG	5 cut(s)	341, 1416, 2100, 2520, 2800
SfuI	TTCGAA	2 cut(s)	550, 2152
SgrAI	CRCCGGYG	1 cut(s)	374
SinI	GGWCC	2 cut(s)	405, 1340
SmiMI	CAYNNNNRTG	1 cut(s)	735
SmlI	CTYRAG	1 cut(s)	602
SmoI	CTYRAG	1 cut(s)	602
SpeI	ACTAGT	1 cut(s)	689
SphI	GCATGC	1 cut(s)	1571
Sse8387I	CCTGCAGG	1 cut(s)	2524
SseBI	AGGCCT	1 cut(s)	2775
SsiI	CCGC	5 cut(s)	32, 272, 2014, 2664, 2700
SspI	AATATT	1 cut(s)	2170
SspMI	CTAG	8 cut(s)	414, 690, 824, 854, 1157, 1604, 1631, 1782
StuI	AGGCCT	1 cut(s)	2775
StyD4I	CCNGG	8 cut(s)	27, 289, 400, 1178, 1345, 1480, 2642, 2775
StyI	CCWWGG	1 cut(s)	2319
TaaI	ACNGT	9 cut(s)	42, 285, 586, 1193, 1397, 1510, 2082, 2443, 2804
TaiI	ACGT	4 cut(s)	315, 841, 1150, 1810
TaqI	TCGA	8 cut(s)	153, 550, 1233, 1408, 1459, 1539, 2152, 2453
TaqII	GACCGA	2 cut(s)	422, 1943
TatI	WGTACW	3 cut(s)	811, 1682, 2558
TauI	GCSGC	2 cut(s)	35, 274
TfiI	GAWTC	5 cut(s)	85, 436, 889, 1705, 2495
TscAI	CASTG	5 cut(s)	288, 889, 1198, 2446, 2554
TseFI	GTSAC	4 cut(s)	580, 1924, 2123, 2389
TseI	GCWGC	7 cut(s)	773, 961, 1108, 1436, 1934, 1993, 2687
Tsp45I	GTSAC	4 cut(s)	580, 1924, 2123, 2389
TspGWI	ACGGA	1 cut(s)	440
TspRI	CASTG	5 cut(s)	288, 889, 1198, 2446, 2554
VneI	GTGCAC	1 cut(s)	1697
VpaK11BI	GGWCC	2 cut(s)	405, 1340
XagI	CCTNNNNNAGG	3 cut(s)	389, 1350, 1664
XapI	RAATTY	4 cut(s)	155, 1133, 1201, 1280
XbaI	TCTAGA	3 cut(s)	1156, 1603, 1630
XceI	RCATGY	5 cut(s)	304, 1388, 1571, 1818, 2135
XmnI	GAANNNNTTC	1 cut(s)	2170
XspI	CTAG	8 cut(s)	414, 690, 824, 854, 1157, 1604, 1631, 1782
ZrmI	AGTACT	1 cut(s)	813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.