ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| LON_substr_bdg | PF02190 | 85 - 290 | 4.3e-36 | ATP-dependent protease La (LON) substrate-binding domain |
| AAA | PF00004 | 443 - 580 | 5.6e-24 | ATPase family associated with various cellular activities (AAA) |
| AAA_5 | PF07728 | 443 - 521 | 6.3e-08 | AAA domain (dynein-related subfamily) |
| Lon_lid | PF22667 | 604 - 655 | 3.5e-18 | Lon protease AAA+ ATPase lid domain |
| Lon_C | PF05362 | 711 - 915 | 7.9e-74 | Lon protease (S16) C-terminal proteolytic domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 638 |
| AarI | CACCTGC | 1 cut(s) | 1883 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 524 |
| Acc36I | ACCTGC | 2 cut(s) | 1883, 2026 |
| AccB1I | GGYRCC | 1 cut(s) | 1472 |
| AciI | CCGC | 5 cut(s) | 272, 1511, 1933, 2583, 2619 |
| AclI | AACGTT | 1 cut(s) | 838 |
| AclWI | GGATC | 7 cut(s) | 314, 327, 569, 582, 1601, 2563, 2576 |
| AcoI | YGGCCR | 1 cut(s) | 535 |
| AcsI | RAATTY | 3 cut(s) | 1133, 1201, 1280 |
| AcuI | CTGAAG | 3 cut(s) | 258, 1278, 2628 |
| AcvI | CACGTG | 1 cut(s) | 1808 |
| AfaI | GTAC | 6 cut(s) | 487, 813, 1494, 1684, 1754, 2479 |
| AfeI | AGCGCT | 1 cut(s) | 1014 |
| AflIII | ACRYGT | 3 cut(s) | 1145, 1805, 2050 |
| AhlI | ACTAGT | 1 cut(s) | 689 |
| AjnI | CCWGG | 5 cut(s) | 400, 1178, 1345, 1480, 2694 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 77, 109, 1968, 2000, 2040, 2072 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 140, 172 |
| Alw21I | GWGCWC | 2 cut(s) | 1701, 2302 |
| Alw26I | GTCTC | 5 cut(s) | 510, 680, 1337, 2007, 2258 |
| Alw44I | GTGCAC | 1 cut(s) | 1697 |
| AlwI | GGATC | 7 cut(s) | 314, 327, 569, 582, 1601, 2563, 2576 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 185 |
| Aor51HI | AGCGCT | 1 cut(s) | 1014 |
| AoxI | GGCC | 6 cut(s) | 97, 535, 1339, 2314, 2462, 2692 |
| ApaI | GGGCCC | 1 cut(s) | 2466 |
| ApaLI | GTGCAC | 1 cut(s) | 1697 |
| ApoI | RAATTY | 3 cut(s) | 1133, 1201, 1280 |
| AspLEI | GCGC | 2 cut(s) | 1015, 1222 |
| AspS9I | GGNCC | 5 cut(s) | 97, 405, 1340, 2462, 2463 |
| AsuC2I | CCSGG | 4 cut(s) | 29, 291, 1886, 2563 |
| AsuHPI | GGTGA | 6 cut(s) | 30, 58, 146, 1855, 2000, 2759 |
| AvaII | GGWCC | 1 cut(s) | 405 |
| AxyI | CCTNAGG | 1 cut(s) | 2454 |
| BaeGI | GKGCMC | 3 cut(s) | 1701, 2347, 2466 |
| BamHI | GGATCC | 3 cut(s) | 319, 574, 2568 |
| BanI | GGYRCC | 1 cut(s) | 1472 |
| BanII | GRGCYC | 1 cut(s) | 2466 |
| BbrPI | CACGTG | 1 cut(s) | 1808 |
| BbsI | GAAGAC | 2 cut(s) | 1805, 2633 |
| Bbv12I | GWGCWC | 2 cut(s) | 1701, 2302 |
| BccI | CCATC | 7 cut(s) | 131, 418, 556, 1483, 1751, 2453, 2639 |
| BceAI | ACGGC | 2 cut(s) | 38, 84 |
| BciT130I | CCWGG | 5 cut(s) | 402, 1180, 1347, 1482, 2696 |
| BclI | TGATCA | 2 cut(s) | 595, 2749 |
| BcnI | CCSGG | 4 cut(s) | 29, 291, 1886, 2563 |
| BcoDI | GTCTC | 5 cut(s) | 510, 680, 1337, 2007, 2258 |
| BcuI | ACTAGT | 1 cut(s) | 689 |
| BfaI | CTAG | 8 cut(s) | 690, 824, 854, 1157, 1604, 1631, 1782, 2082 |
| BfmI | CTRYAG | 5 cut(s) | 341, 1416, 2019, 2439, 2719 |
| BfoI | RGCGCY | 1 cut(s) | 1016 |
| BfuAI | ACCTGC | 2 cut(s) | 1883, 2026 |
| BlpI | GCTNAGC | 1 cut(s) | 1097 |
| BmcAI | AGTACT | 1 cut(s) | 813 |
| Bme1390I | CCNGG | 9 cut(s) | 29, 291, 402, 1180, 1347, 1482, 1886, 2563, 2696 |
| Bme18I | GGWCC | 1 cut(s) | 405 |
| BmgT120I | GGNCC | 5 cut(s) | 97, 405, 1340, 2462, 2463 |
| BmiI | GGNNCC | 7 cut(s) | 321, 406, 576, 1474, 1904, 2464, 2570 |
| BmrFI | CCNGG | 9 cut(s) | 29, 291, 402, 1180, 1347, 1482, 1886, 2563, 2696 |
| BmrI | ACTGGG | 3 cut(s) | 995, 2460, 2553 |
| BmsI | GCATC | 4 cut(s) | 905, 1108, 1462, 1840 |
| BmuI | ACTGGG | 3 cut(s) | 995, 2460, 2553 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2721 |
| BpiI | GAAGAC | 2 cut(s) | 1805, 2633 |
| BpmI | CTGGAG | 3 cut(s) | 704, 1368, 1892 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1097 |
| BpuEI | CTTGAG | 1 cut(s) | 587 |
| BpuMI | CCSGG | 4 cut(s) | 29, 291, 1886, 2563 |
| Bsa29I | ATCGAT | 2 cut(s) | 1539, 2372 |
| BsaAI | YACGTR | 2 cut(s) | 1148, 1808 |
| BsaBI | GATNNNNATC | 3 cut(s) | 573, 878, 1239 |
| BsaI | GGTCTC | 2 cut(s) | 510, 2258 |
| BsaJI | CCNNGG | 5 cut(s) | 27, 401, 1178, 1179, 2238 |
| BsaWI | WCCGGW | 2 cut(s) | 2203, 2289 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 30, 60, 507, 537 |
| Bse118I | RCCGGY | 4 cut(s) | 68, 357, 374, 1978 |
| Bse1I | ACTGG | 4 cut(s) | 990, 1122, 2466, 2548 |
| Bse21I | CCTNAGG | 1 cut(s) | 2454 |
| Bse3DI | GCAATG | 5 cut(s) | 260, 266, 806, 1776, 2544 |
| Bse8I | GATNNNNATC | 3 cut(s) | 573, 878, 1239 |
| BseBI | CCWGG | 5 cut(s) | 402, 1180, 1347, 1482, 2696 |
| BseCI | ATCGAT | 2 cut(s) | 1539, 2372 |
| BseDI | CCNNGG | 5 cut(s) | 27, 401, 1178, 1179, 2238 |
| BseGI | GGATG | 8 cut(s) | 400, 631, 1123, 1576, 2332, 2464, 2489, 2704 |
| BseJI | GATNNNNATC | 3 cut(s) | 573, 878, 1239 |
| BseMI | GCAATG | 5 cut(s) | 260, 266, 806, 1776, 2544 |
| BseMII | CTCAG | 6 cut(s) | 177, 411, 740, 1315, 2022, 2285 |
| BseNI | ACTGG | 4 cut(s) | 990, 1122, 2466, 2548 |
| BseRI | GAGGAG | 3 cut(s) | 1704, 2626, 2681 |
| BseSI | GKGCMC | 3 cut(s) | 1701, 2347, 2466 |
| BseYI | CCCAGC | 1 cut(s) | 1748 |
| BshFI | GGCC | 6 cut(s) | 99, 537, 1341, 2316, 2464, 2694 |
| BshNI | GGYRCC | 1 cut(s) | 1472 |
| BshVI | ATCGAT | 2 cut(s) | 1539, 2372 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1701, 2302 |
| BslFI | GGGAC | 1 cut(s) | 418 |
| BsmAI | GTCTC | 5 cut(s) | 510, 680, 1337, 2007, 2258 |
| BsmFI | GGGAC | 1 cut(s) | 418 |
| BsmI | GAATGC | 1 cut(s) | 507 |
| BsnI | GGCC | 6 cut(s) | 99, 537, 1341, 2316, 2464, 2694 |
| Bso31I | GGTCTC | 2 cut(s) | 510, 2258 |
| Bsp120I | GGGCCC | 1 cut(s) | 2462 |
| Bsp1286I | GDGCHC | 4 cut(s) | 1701, 2302, 2347, 2466 |
| Bsp1407I | TGTACA | 1 cut(s) | 1682 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1097 |
| Bsp19I | CCATGG | 1 cut(s) | 2238 |
| BspACI | CCGC | 5 cut(s) | 272, 1511, 1933, 2583, 2619 |
| BspANI | GGCC | 6 cut(s) | 99, 537, 1341, 2316, 2464, 2694 |
| BspCNI | CTCAG | 6 cut(s) | 178, 412, 739, 1314, 2021, 2284 |
| BspDI | ATCGAT | 2 cut(s) | 1539, 2372 |
| BspHI | TCATGA | 1 cut(s) | 2331 |
| BspLI | GGNNCC | 7 cut(s) | 321, 406, 576, 1474, 1904, 2464, 2570 |
| BspMAI | CTGCAG | 2 cut(s) | 2023, 2443 |
| BspMI | ACCTGC | 2 cut(s) | 1883, 2026 |
| BspPI | GGATC | 7 cut(s) | 314, 327, 569, 582, 1601, 2563, 2576 |
| BspQI | GCTCTTC | 3 cut(s) | 813, 1086, 1863 |
| BspT107I | GGYRCC | 1 cut(s) | 1472 |
| BspTNI | GGTCTC | 2 cut(s) | 510, 2258 |
| BsrDI | GCAATG | 5 cut(s) | 260, 266, 806, 1776, 2544 |
| BsrFI | RCCGGY | 4 cut(s) | 68, 357, 374, 1978 |
| BsrGI | TGTACA | 1 cut(s) | 1682 |
| BsrI | ACTGG | 4 cut(s) | 990, 1122, 2466, 2548 |
| BssAI | RCCGGY | 4 cut(s) | 68, 357, 374, 1978 |
| BssECI | CCNNGG | 5 cut(s) | 27, 401, 1178, 1179, 2238 |
| BssT1I | CCWWGG | 1 cut(s) | 2238 |
| Bst2UI | CCWGG | 5 cut(s) | 402, 1180, 1347, 1482, 2696 |
| Bst6I | CTCTTC | 4 cut(s) | 813, 912, 1086, 1863 |
| BstAUI | TGTACA | 1 cut(s) | 1682 |
| BstBAI | YACGTR | 2 cut(s) | 1148, 1808 |
| BstC8I | GCNNGC | 3 cut(s) | 492, 1339, 1569 |
| BstDSI | CCRYGG | 1 cut(s) | 2238 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 389, 1350, 1664 |
| BstF5I | GGATG | 8 cut(s) | 400, 631, 1123, 1576, 2332, 2464, 2489, 2704 |
| BstH2I | RGCGCY | 1 cut(s) | 1016 |
| BstHHI | GCGC | 2 cut(s) | 1015, 1222 |
| BstMAI | GTCTC | 5 cut(s) | 510, 680, 1337, 2007, 2258 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 78, 271, 829, 1517, 1862, 2234, 2351 |
| BstNI | CCWGG | 5 cut(s) | 402, 1180, 1347, 1482, 2696 |
| BstNSI | RCATGY | 5 cut(s) | 304, 1388, 1571, 1818, 2054 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2721 |
| BstSCI | CCNGG | 9 cut(s) | 27, 289, 400, 1178, 1345, 1480, 1884, 2561, 2694 |
| BstSFI | CTRYAG | 5 cut(s) | 341, 1416, 2019, 2439, 2719 |
| BstSLI | GKGCMC | 3 cut(s) | 1701, 2347, 2466 |
| BstV2I | GAAGAC | 2 cut(s) | 1805, 2633 |
| BstX2I | RGATCY | 4 cut(s) | 319, 574, 1606, 2568 |
| BstYI | RGATCY | 4 cut(s) | 319, 574, 1606, 2568 |
| Bsu15I | ATCGAT | 2 cut(s) | 1539, 2372 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2454 |
| BsuRI | GGCC | 6 cut(s) | 99, 537, 1341, 2316, 2464, 2694 |
| BsuTUI | ATCGAT | 2 cut(s) | 1539, 2372 |
| BtgI | CCRYGG | 1 cut(s) | 2238 |
| BtgZI | GCGATG | 1 cut(s) | 1729 |
| BtsCI | GGATG | 8 cut(s) | 400, 631, 1123, 1576, 2332, 2464, 2489, 2704 |
| BtsIMutI | CAGTG | 4 cut(s) | 281, 1198, 2358, 2473 |
| BveI | ACCTGC | 2 cut(s) | 1883, 2026 |
| Cac8I | GCNNGC | 3 cut(s) | 492, 1339, 1569 |
| CaiI | CAGNNNCTG | 1 cut(s) | 185 |
| CciI | TCATGA | 1 cut(s) | 2331 |
| CfoI | GCGC | 2 cut(s) | 1015, 1222 |
| Cfr10I | RCCGGY | 4 cut(s) | 68, 357, 374, 1978 |
| Cfr13I | GGNCC | 5 cut(s) | 97, 405, 1340, 2462, 2463 |
| ClaI | ATCGAT | 2 cut(s) | 1539, 2372 |
| CseI | GACGC | 3 cut(s) | 131, 929, 1442 |
| Csp6I | GTAC | 6 cut(s) | 486, 812, 1493, 1683, 1753, 2478 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2257, 2292 |
| CviQI | GTAC | 6 cut(s) | 486, 812, 1493, 1683, 1753, 2478 |
| DraI | TTTAAA | 1 cut(s) | 843 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 524 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 524 |
| EaeI | YGGCCR | 1 cut(s) | 535 |
| Eam1104I | CTCTTC | 4 cut(s) | 813, 912, 1086, 1863 |
| EarI | CTCTTC | 4 cut(s) | 813, 912, 1086, 1863 |
| EciI | GGCGGA | 1 cut(s) | 2634 |
| Eco130I | CCWWGG | 1 cut(s) | 2238 |
| Eco147I | AGGCCT | 1 cut(s) | 2694 |
| Eco24I | GRGCYC | 1 cut(s) | 2466 |
| Eco31I | GGTCTC | 2 cut(s) | 510, 2258 |
| Eco47I | GGWCC | 1 cut(s) | 405 |
| Eco47III | AGCGCT | 1 cut(s) | 1014 |
| Eco57I | CTGAAG | 3 cut(s) | 258, 1278, 2628 |
| Eco72I | CACGTG | 1 cut(s) | 1808 |
| Eco81I | CCTNAGG | 1 cut(s) | 2454 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 389, 1350, 1664 |
| EcoRII | CCWGG | 5 cut(s) | 400, 1178, 1345, 1480, 2694 |
| EcoT14I | CCWWGG | 1 cut(s) | 2238 |
| EcoT38I | GRGCYC | 1 cut(s) | 2466 |
| ErhI | CCWWGG | 1 cut(s) | 2238 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1080, 1112, 2286, 2318 |
| FaqI | GGGAC | 1 cut(s) | 418 |
| FauI | CCCGC | 2 cut(s) | 1504, 2576 |
| FauNDI | CATATG | 3 cut(s) | 370, 616, 652 |
| FbaI | TGATCA | 2 cut(s) | 595, 2749 |
| FokI | GGATG | 8 cut(s) | 407, 638, 1130, 1563, 2339, 2471, 2476, 2711 |
| FriOI | GRGCYC | 1 cut(s) | 2466 |
| FspBI | CTAG | 8 cut(s) | 690, 824, 854, 1157, 1604, 1631, 1782, 2082 |
| GlaI | GCGC | 2 cut(s) | 1014, 1221 |
| GsaI | CCCAGC | 1 cut(s) | 1752 |
| GsuI | CTGGAG | 3 cut(s) | 704, 1368, 1892 |
| HaeII | RGCGCY | 1 cut(s) | 1016 |
| HaeIII | GGCC | 6 cut(s) | 99, 537, 1341, 2316, 2464, 2694 |
| HgaI | GACGC | 3 cut(s) | 131, 929, 1442 |
| HhaI | GCGC | 2 cut(s) | 1015, 1222 |
| Hin6I | GCGC | 2 cut(s) | 1013, 1220 |
| HinP1I | GCGC | 2 cut(s) | 1013, 1220 |
| HincII | GTYRAC | 3 cut(s) | 1428, 2134, 2524 |
| HindII | GTYRAC | 3 cut(s) | 1428, 2134, 2524 |
| HindIII | AAGCTT | 1 cut(s) | 2420 |
| HinfI | GANTC | 8 cut(s) | 85, 188, 889, 1705, 1926, 2135, 2200, 2414 |
| HpaI | GTTAAC | 1 cut(s) | 1428 |
| HphI | GGTGA | 6 cut(s) | 30, 58, 146, 1855, 2000, 2759 |
| Hpy166II | GTNNAC | 5 cut(s) | 1428, 1699, 2134, 2524, 2704 |
| Hpy188I | TCNGA | 9 cut(s) | 50, 121, 421, 934, 1304, 1315, 1536, 2011, 2727 |
| Hpy8I | GTNNAC | 5 cut(s) | 1428, 1699, 2134, 2524, 2704 |
| Hpy99I | CGWCG | 3 cut(s) | 105, 945, 1461 |
| HpyAV | CCTTC | 9 cut(s) | 30, 310, 973, 1219, 1310, 1554, 2582, 2652, 2684 |
| HpyCH4IV | ACGT | 3 cut(s) | 838, 1147, 1807 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 78, 271, 829, 1517, 1862, 2234, 2351 |
| HpySE526I | ACGT | 3 cut(s) | 838, 1147, 1807 |
| HspAI | GCGC | 2 cut(s) | 1013, 1220 |
| Ksp22I | TGATCA | 2 cut(s) | 595, 2749 |
| KspAI | GTTAAC | 1 cut(s) | 1428 |
| LguI | GCTCTTC | 3 cut(s) | 813, 1086, 1863 |
| LmnI | GCTCC | 4 cut(s) | 1597, 1964, 2297, 2668 |
| LweI | GCATC | 4 cut(s) | 905, 1108, 1462, 1840 |
| MaeI | CTAG | 8 cut(s) | 690, 824, 854, 1157, 1604, 1631, 1782, 2082 |
| MaeII | ACGT | 3 cut(s) | 838, 1147, 1807 |
| MaeIII | GTNAC | 4 cut(s) | 431, 1843, 2308, 2551 |
| MfeI | CAATTG | 3 cut(s) | 1377, 1653, 2577 |
| MflI | RGATCY | 4 cut(s) | 319, 574, 1606, 2568 |
| MhlI | GDGCHC | 4 cut(s) | 1701, 2302, 2347, 2466 |
| MlyI | GAGTC | 3 cut(s) | 197, 2129, 2194 |
| MmeI | TCCRAC | 6 cut(s) | 1095, 1273, 1575, 1701, 1733, 2188 |
| MseI | TTAA | 9 cut(s) | 231, 842, 953, 966, 1137, 1253, 1427, 1446, 1503 |
| MslI | CAYNNNNRTG | 1 cut(s) | 735 |
| MspA1I | CMGCKG | 2 cut(s) | 1111, 1439 |
| MspR9I | CCNGG | 9 cut(s) | 29, 291, 402, 1180, 1347, 1482, 1886, 2563, 2696 |
| MunI | CAATTG | 3 cut(s) | 1377, 1653, 2577 |
| Mva1269I | GAATGC | 1 cut(s) | 507 |
| MvaI | CCWGG | 5 cut(s) | 402, 1180, 1347, 1482, 2696 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 78, 271, 829, 1517, 1862, 2234, 2351 |
| NciI | CCSGG | 4 cut(s) | 29, 291, 1886, 2563 |
| NcoI | CCATGG | 1 cut(s) | 2238 |
| NdeI | CATATG | 3 cut(s) | 370, 616, 652 |
| NlaIV | GGNNCC | 7 cut(s) | 321, 406, 576, 1474, 1904, 2464, 2570 |
| NmuCI | GTSAC | 2 cut(s) | 1843, 2308 |
| NspI | RCATGY | 5 cut(s) | 304, 1388, 1571, 1818, 2054 |
| PaeI | GCATGC | 1 cut(s) | 1571 |
| PagI | TCATGA | 1 cut(s) | 2331 |
| PaqCI | CACCTGC | 1 cut(s) | 1883 |
| PasI | CCCWGGG | 1 cut(s) | 1179 |
| PceI | AGGCCT | 1 cut(s) | 2694 |
| PciI | ACATGT | 1 cut(s) | 2050 |
| PciSI | GCTCTTC | 3 cut(s) | 813, 1086, 1863 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 203, 2751 |
| PctI | GAATGC | 1 cut(s) | 507 |
| PfeI | GAWTC | 5 cut(s) | 85, 889, 1705, 1926, 2414 |
| PleI | GAGTC | 3 cut(s) | 196, 2129, 2194 |
| PmaCI | CACGTG | 1 cut(s) | 1808 |
| PmlI | CACGTG | 1 cut(s) | 1808 |
| PpsI | GAGTC | 3 cut(s) | 196, 2129, 2194 |
| Ppu21I | YACGTR | 2 cut(s) | 1148, 1808 |
| PscI | ACATGT | 1 cut(s) | 2050 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2721 |
| PsiI | TTATAA | 1 cut(s) | 638 |
| Psp1406I | AACGTT | 1 cut(s) | 838 |
| Psp6I | CCWGG | 5 cut(s) | 400, 1178, 1345, 1480, 2694 |
| PspCI | CACGTG | 1 cut(s) | 1808 |
| PspFI | CCCAGC | 1 cut(s) | 1748 |
| PspGI | CCWGG | 5 cut(s) | 400, 1178, 1345, 1480, 2694 |
| PspN4I | GGNNCC | 7 cut(s) | 321, 406, 576, 1474, 1904, 2464, 2570 |
| PspOMI | GGGCCC | 1 cut(s) | 2462 |
| PspPI | GGNCC | 5 cut(s) | 97, 405, 1340, 2462, 2463 |
| PstI | CTGCAG | 2 cut(s) | 2023, 2443 |
| PstNI | CAGNNNCTG | 1 cut(s) | 185 |
| PsuI | RGATCY | 4 cut(s) | 319, 574, 1606, 2568 |
| PvuII | CAGCTG | 2 cut(s) | 1111, 1439 |
| RsaI | GTAC | 6 cut(s) | 487, 813, 1494, 1684, 1754, 2479 |
| RsaNI | GTAC | 6 cut(s) | 486, 812, 1493, 1683, 1753, 2478 |
| RseI | CAYNNNNRTG | 1 cut(s) | 735 |
| SapI | GCTCTTC | 3 cut(s) | 813, 1086, 1863 |
| SaqAI | TTAA | 9 cut(s) | 231, 842, 953, 966, 1137, 1253, 1427, 1446, 1503 |
| Sau96I | GGNCC | 5 cut(s) | 97, 405, 1340, 2462, 2463 |
| SbfI | CCTGCAGG | 1 cut(s) | 2443 |
| ScaI | AGTACT | 1 cut(s) | 813 |
| SchI | GAGTC | 3 cut(s) | 197, 2129, 2194 |
| ScrFI | CCNGG | 9 cut(s) | 29, 291, 402, 1180, 1347, 1482, 1886, 2563, 2696 |
| SdaI | CCTGCAGG | 1 cut(s) | 2443 |
| SduI | GDGCHC | 4 cut(s) | 1701, 2302, 2347, 2466 |
| SfaNI | GCATC | 4 cut(s) | 905, 1108, 1462, 1840 |
| SfcI | CTRYAG | 5 cut(s) | 341, 1416, 2019, 2439, 2719 |
| SinI | GGWCC | 1 cut(s) | 405 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 735 |
| SmlI | CTYRAG | 1 cut(s) | 602 |
| SmoI | CTYRAG | 1 cut(s) | 602 |
| SpeI | ACTAGT | 1 cut(s) | 689 |
| SphI | GCATGC | 1 cut(s) | 1571 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 2443 |
| SseBI | AGGCCT | 1 cut(s) | 2694 |
| SsiI | CCGC | 5 cut(s) | 272, 1511, 1933, 2583, 2619 |
| SspI | AATATT | 1 cut(s) | 2089 |
| SspMI | CTAG | 8 cut(s) | 690, 824, 854, 1157, 1604, 1631, 1782, 2082 |
| StuI | AGGCCT | 1 cut(s) | 2694 |
| StyD4I | CCNGG | 9 cut(s) | 27, 289, 400, 1178, 1345, 1480, 1884, 2561, 2694 |
| StyI | CCWWGG | 1 cut(s) | 2238 |
| TaiI | ACGT | 3 cut(s) | 841, 1150, 1810 |
| TaqI | TCGA | 7 cut(s) | 103, 153, 1233, 1408, 1459, 1539, 2372 |
| TaqII | GACCGA | 1 cut(s) | 422 |
| TatI | WGTACW | 3 cut(s) | 811, 1682, 2477 |
| TauI | GCSGC | 1 cut(s) | 274 |
| TfiI | GAWTC | 5 cut(s) | 85, 889, 1705, 1926, 2414 |
| Tru1I | TTAA | 9 cut(s) | 231, 842, 953, 966, 1137, 1253, 1427, 1446, 1503 |
| Tru9I | TTAA | 9 cut(s) | 231, 842, 953, 966, 1137, 1253, 1427, 1446, 1503 |
| TscAI | CASTG | 4 cut(s) | 288, 1198, 2365, 2473 |
| TseFI | GTSAC | 2 cut(s) | 1843, 2308 |
| Tsp45I | GTSAC | 2 cut(s) | 1843, 2308 |
| TspRI | CASTG | 4 cut(s) | 288, 1198, 2365, 2473 |
| VneI | GTGCAC | 1 cut(s) | 1697 |
| VpaK11BI | GGWCC | 1 cut(s) | 405 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 389, 1350, 1664 |
| XapI | RAATTY | 3 cut(s) | 1133, 1201, 1280 |
| XbaI | TCTAGA | 3 cut(s) | 1156, 1603, 1630 |
| XceI | RCATGY | 5 cut(s) | 304, 1388, 1571, 1818, 2054 |
| XspI | CTAG | 8 cut(s) | 690, 824, 854, 1157, 1604, 1631, 1782, 2082 |
| ZrmI | AGTACT | 1 cut(s) | 813 |