Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3A

Orthogroup ID

OG0001800

Physical Location & Seq

Genomic Coordinates Reverse (-)

6214489 .. 6224696

Gene Structure 10208 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3AG078300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2982 bp

ATGTTGAAGCTTCTCTCTTCTTCCTCCTCGTGCCTACAGGCCGGAGCCCACAGTTTGGGCCCTAAATTGGGCGTCCGGCCCGAAATGACTTCGCCGTTACTCCGAGTTCTGAACTCCCTCACCGGCCGGAATCGGCCAAATATGGCTCGCCGGGCGTTTTTTTGCTCGGATTCAAGCGACGGCTCCGATCAAGTTGTTGAGTTGGAGGCCAAGGCTGGGACTGAGGCTGAGGCTAAGGCTTCTTCAGCTATAGTGTCCACAAACCCTAGACCAGAAGATTATCTAACGGTTTTAGCTTTGCCATTGCCGCATAGACCACTCTTTCCAGGGTTCTACATGCCAATTTATGTGAAGGATCCAAAATTGCTAGCGGCTTTACAGGAAAGCCGGAAACGGCAAGCTCCATATGCTGGAGCTTTTCTGGTGAAGGATGAGCCAGGGACTGATCCAAGTTTGGTATCAGGTTCTGATACAGAGAAAAGCATATATGAATTAAAAGGGAAAGCCTTGTTTAACCGTTTGCATGAAGTTGGTACGCTTGCCCAGATTTCTAGTATCCAAGGTGACCAAGTAGTGCTTATTGGTCATAGGCGACTTCGAATTACAGAGATGGTTGATGAGGACCCCCTGACTGTAAAAGTTGACCATCTCAAGAATAAACCATATAACAAGGATGATGATGTCATAAAGGCTACGTCATTTGAGGTTATAGCAACCTTAAGAGATGTTCTGAAGACTAGTTCTCTTTGGAGAGATCATGTTCAAACATACACTCAGCATATAGGTGACTTTAATTTTCCGAGGTTAGCAGATTTTGGAGCTGCAATATCTGGTGCAAACAAATTGCAGTGCCAGCAAGTGCTTGAAGAGCTAGACGTGTACAAACGTTTAAATCTTACACTAGAATTGGTGAAGAAAGAGATGGAAATCAGCAAGCTTCAGGAAACAATTGCAAAAGCGATTGAAGAGAAGATAAGCCGTGAGCAACGCCGCTACTTGTTGAATGAGCAGCTTAAAGCCATAAAGAAGGAACTGGGATTAGAGACAGATGACAAGACGGCACTTTCTGCAAAGTTTAGGGAGAGGCTTGAACCAAACAAGGATAAATGTCCACCTCATGTTTTGCAAGTTATAGAAGAAGAGCTTGCAAAACTACAGCTGCTGGAGGCCAGTTCGAGTGAATTTAACGTGACACGTAATTACCTAGATTGGTTGACGTCAATTCCCTGGGGAAATTACAGTAATGAGAACTTTGATGTTCTTCGGGCTGAAAAAATTCTTGATGAAGACCATTACGGATTAAATGATGTAAAGGAAAGGATATTGGAATTTATTGCTGTTGGAAAACTCAGAGGAATCTCACAAGGAAAAATCATTTGTCTCTCTGGCCCTCCTGGAGTTGGCAAAACCAGCATTGGTCGTTCAATTGCACGTGCATTGAATCGCAATTTCTTTCGATTTTCTGTTGGAGGGTTATCTGATGTCGCTGAAATTAAGGGACATCGCCGAACCTATATCGGTGCTATGCCAGGAAAGATGGTTCAATGCCTTAAAAATGTTGGAACAGCTAACCCTCTAGTTCTAATTGATGAGATCGACAAGTTGGGAATTGGACATGCTGGTGATCCGGCTAGTGCTTTGTTGGAGCTTCTTGATCCAGAGCAAAATGCTAATTTTCTAGATCATTATCTTGATGTTCCAATCGACCTAACAAAGGTTCTGTTTGTTTGTACAGCAAATGTTGTCGAGAATATACCAGCTCCTCTCTTGGATAGAATGGAGGTCATTTCCATAGCTGGGTATATTACTGATGAGAAAATGCACATTGCTAGAGACTATTTGGAGAAGACGACACGTGAAGCATGCGGCATCGAACCTGAACAGGTTGAAGTGACCAATGCAGCACTTCTTGCTCTCGTAGAAAATTATTGCCGAGAAGCAGGTGTTCGGAACCTCCAAAAGCACATAGAAAAGATCTACCGTAAGATAGCTTTGCAACTTGTTAGGCAAGGACAGTCAGATGAACCTGTAGATGCTGGCGAAGTACAGTCTCCCGTCAGTAAAACTGATCTCCCAGATGAACCTGCAGTTGTTGCAGATACTGATGAAGCCAAAGTAGAAGATTTTGCTGGCGTAAAACAGACTGCAGATGAATCAGCAGAGGGTAAAGACCATGAAGATAATACTGAAACCTCTGTGGGAAGTGTCACTATCCCAGATGAAACTGGAGCTTCTGAAGTTCAGGTTGCAGATACTGGTGAACTTATGCATTCAAAGGATACAGAAGAAACTCAGAAAATTCAGGAGGGTGAAGCCAAAAAACCAGTTGAGAAAGTGTTGGTTAATGAATCAAACTTGGGTGACTTTGTTGGCAAACCAGTTTTCCATGCGGATCGCATCTATGAACAGACTCCGGTTGGAGTTGTCATGGGTCTGGCTTGGACTGCCATGGGTGGTTCCACCCTGTATATAGAGACCACTCAGGTCGAGGAAGGAGAGGGAAAAGGAGCGCTTCATGCTACTGGTCAACTTGGAGATGTTATGAAAGAAAGTACCCAAATTGCTCACACTGTGGCCAGAGCAATATTGCTCGAGAAAGAGCCAGAGAACACTTTCTTTGCCGATTCCAAGCTTCATCTCCATGTGCCGGCAGGGGCCACACCAAAGGATGGGCCAAGTGCTGGTTGCACCATGATCACATCCATGCTGTCCATTGCCATGAAGAAGCCTGTCAAGAAGGATTTAGCAATGACAGGGGAAGTAACACTAACTGGCAGGATCCTTCCAATTGGCGGGGTTAAGGAGAAGACAATAGCAGCGAGGCGGAGTGGAGTGAAGACCATCATATTCCCTGAAGCCAACAGGAAGGATTTTGATGAGCTTGCCCCTAATGTAAAAGAAGGTCTTGATGTTCACTTTGTAGATGACTACAATCAGATATTCGCTTTGGCTTTCAGTGATGACCAGAATCAAGAAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004176 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0006508 GO:0006515 GO:0006807 GO:0006996 GO:0007005 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019538 GO:0030163 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0042623 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0051603 GO:0070011 GO:0070013 GO:0071704 GO:0071840 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA LON_substr_bdg Lon_C

Protein Analysis

993

Amino Acids

109.35

Weight (kDa)

5.28

Isoelectric Point (pI)

39.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LON_substr_bdg	PF02190	97 - 306	3.9e-36	ATP-dependent protease La (LON) substrate-binding domain
AAA	PF00004	459 - 596	7.2e-23	ATPase family associated with various cellular activities (AAA)
Lon_lid	PF22667	620 - 671	4.2e-18	Lon protease AAA+ ATPase lid domain
Lon_C	PF05362	781 - 985	1.8e-76	Lon protease (S16) C-terminal proteolytic domain
ChlI	PF13541	833 - 954	8.2e-06	Subunit ChlI of Mg-chelatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001800)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05790 AT3G05790 AT5G26860
fragaria_vesca	FvH4_6g07420 FvH4_7g03300 FvH4_7g03300 FvH4_7g03300
malus_domestica	MD02G1284000.v1.1 MD04G1193200.v1.1 MD07G1050000.v1.1 MD12G1206100.v1.1
prunus_persica	Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.2G038600_v2.0.a1 Prupe.6G313300_v2.0.a1
pyrus_communis	pycom02g24200 pycom04g17100 pycom07g03580 pycom12g19400
rosa_chinensis	RchiOBHm_Chr1g0325611 RchiOBHm_Chr3g0457021
rosa_laevigata	RLG00000025251 RLG00000030117
rosa_multiflora	Rmu_sc0000488.1_g000001
rosa_roxburghii	Rroxscaffold_4G00324580 Rroxscaffold_6G00422260
rosa_rugosa	Rorug01G0053100 Rorug01G0053200 Rorug01G0053200 Rorug03G0018600 Rorug03G0018700
rosa_samantha	Rh1AG068800 Rh1CG069200 Rh1DG073900 Rh3AG078300 Rh3BG080200 Rh3CG080100 Rh3DG081900
rosa_wichuraiana	Rw1G005700 Rw3G006720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1931
AatII	GACGTC	1 cut(s)	1220
Acc36I	ACCTGC	2 cut(s)	1931, 2092
AccB7I	CCANNNNNTGG	4 cut(s)	55, 410, 2669, 2681
AciI	CCGC	7 cut(s)	308, 371, 991, 1866, 2390, 2793, 2824
AclI	AACGTT	1 cut(s)	886
AclWI	GGATC	8 cut(s)	350, 363, 440, 1619, 1649, 2400, 2773, 2786
AcoI	YGGCCR	3 cut(s)	124, 134, 2574
AcsI	RAATTY	4 cut(s)	1181, 1275, 1328, 2298
AcuI	CTGAAG	5 cut(s)	228, 752, 923, 2256, 2874
AcvI	CACGTG	2 cut(s)	1433, 1856
AcyI	GRCGYC	2 cut(s)	72, 1217
AdeI	CACNNNGTG	1 cut(s)	2572
AfaI	GTAC	5 cut(s)	535, 881, 1732, 2046, 2554
AfeI	AGCGCT	1 cut(s)	2511
AflII	CTTAAG	1 cut(s)	718
AflIII	ACRYGT	3 cut(s)	876, 1193, 1853
AhlI	ACTAGT	1 cut(s)	737
AjiI	CACGTC	1 cut(s)	877
AjnI	CCWGG	5 cut(s)	325, 436, 1226, 1393, 1528
AloI	GAACNNNNNNTCC	2 cut(s)	1524, 1556
Alw26I	GTCTC	5 cut(s)	1037, 1385, 1827, 2055, 2468
AlwI	GGATC	8 cut(s)	350, 363, 440, 1619, 1649, 2400, 2773, 2786
AlwNI	CAGNNNCTG	5 cut(s)	443, 467, 1162, 2102, 2255
Ama87I	CYCGRG	1 cut(s)	2591
Aor51HI	AGCGCT	1 cut(s)	2511
ApaI	GGGCCC	1 cut(s)	62
ApeKI	GCWGC	5 cut(s)	821, 1009, 1159, 1901, 2816
ApoI	RAATTY	4 cut(s)	1181, 1275, 1328, 2298
Asp700I	GAANNNNTTC	2 cut(s)	1275, 2612
AspLEI	GCGC	1 cut(s)	2512
AspS9I	GGNCC	7 cut(s)	58, 59, 78, 622, 1388, 2655, 2672
AsuC2I	CCSGG	1 cut(s)	152
AsuHPI	GGTGA	9 cut(s)	112, 436, 575, 797, 922, 1634, 2270, 2321, 2372
AsuII	TTCGAA	1 cut(s)	598
AsuNHI	GCTAGC	1 cut(s)	367
AvaI	CYCGRG	1 cut(s)	2591
AvaII	GGWCC	1 cut(s)	622
BaeGI	GKGCMC	1 cut(s)	62
BalI	TGGCCA	1 cut(s)	2576
BamHI	GGATCC	2 cut(s)	355, 2778
BanII	GRGCYC	2 cut(s)	49, 62
BauI	CACGAG	1 cut(s)	28
BbrPI	CACGTG	2 cut(s)	1433, 1856
BbsI	GAAGAC	5 cut(s)	740, 1293, 1853, 2813, 2843
BbvCI	CCTCAGC	1 cut(s)	228
BbvI	GCAGC	5 cut(s)	808, 1021, 1146, 1913, 2828
BccI	CCATC	6 cut(s)	604, 654, 916, 1531, 2663, 2849
BceAI	ACGGC	5 cut(s)	79, 196, 410, 963, 1074
BciT130I	CCWGG	5 cut(s)	327, 438, 1228, 1395, 1530
BciVI	GTATCC	2 cut(s)	566, 2272
BclI	TGATCA	1 cut(s)	2694
BcnI	CCSGG	1 cut(s)	152
BcoDI	GTCTC	5 cut(s)	1037, 1385, 1827, 2055, 2468
BcuI	ACTAGT	1 cut(s)	737
BfmI	CTRYAG	6 cut(s)	35, 249, 1154, 2028, 2085, 2145
BfoI	RGCGCY	1 cut(s)	2513
BfrI	CTTAAG	1 cut(s)	718
BfuAI	ACCTGC	2 cut(s)	1931, 2092
BfuI	GTATCC	2 cut(s)	566, 2272
BglII	AGATCT	1 cut(s)	1974
BisI	GCNGC	9 cut(s)	308, 372, 822, 991, 1010, 1160, 1867, 1902, 2817
BlsI	GCNGC	9 cut(s)	309, 373, 823, 992, 1011, 1161, 1868, 1903, 2818
Bme1390I	CCNGG	6 cut(s)	152, 327, 438, 1228, 1395, 1530
Bme18I	GGWCC	1 cut(s)	622
BmeT110I	CYCGRG	1 cut(s)	2591
BmgBI	CACGTC	1 cut(s)	877
BmgT120I	GGNCC	7 cut(s)	58, 59, 78, 622, 1388, 2655, 2672
BmiI	GGNNCC	9 cut(s)	46, 60, 184, 357, 624, 1952, 2458, 2656, 2780
BmrFI	CCNGG	6 cut(s)	152, 327, 438, 1228, 1395, 1530
BmrI	ACTGGG	1 cut(s)	1043
BmsI	GCATC	3 cut(s)	1878, 2023, 2406
BmtI	GCTAGC	1 cut(s)	371
BmuI	ACTGGG	1 cut(s)	1043
BpiI	GAAGAC	5 cut(s)	740, 1293, 1853, 2813, 2843
BpmI	CTGGAG	4 cut(s)	432, 1184, 1416, 2247
Bpu10I	CCTNAGC	2 cut(s)	228, 234
Bpu14I	TTCGAA	1 cut(s)	598
BpuEI	CTTGAG	1 cut(s)	635
BpuMI	CCSGG	1 cut(s)	152
BsaAI	YACGTR	3 cut(s)	1196, 1433, 1856
BsaBI	GATNNNNATC	2 cut(s)	926, 1686
BsaHI	GRCGYC	2 cut(s)	72, 1217
BsaI	GGTCTC	1 cut(s)	2468
BsaJI	CCNNGG	8 cut(s)	210, 326, 437, 559, 800, 1226, 1227, 2448
BsaWI	WCCGGW	1 cut(s)	2413
Bse118I	RCCGGY	2 cut(s)	122, 2647
Bse1I	ACTGG	8 cut(s)	1038, 1170, 2230, 2260, 2324, 2378, 2527, 2776
Bse3DI	GCAATG	4 cut(s)	302, 1824, 2712, 2754
Bse8I	GATNNNNATC	2 cut(s)	926, 1686
BseBI	CCWGG	5 cut(s)	327, 438, 1228, 1395, 1530
BseDI	CCNNGG	8 cut(s)	210, 326, 437, 559, 800, 1226, 1227, 2448
BseGI	GGATG	4 cut(s)	436, 679, 2674, 2699
BseJI	GATNNNNATC	2 cut(s)	926, 1686
BseMI	GCAATG	4 cut(s)	302, 1824, 2712, 2754
BseMII	CTCAG	6 cut(s)	213, 219, 788, 1363, 2306, 2495
BseNI	ACTGG	8 cut(s)	1038, 1170, 2230, 2260, 2324, 2378, 2527, 2776
BseRI	GAGGAG	2 cut(s)	16, 1752
BseSI	GKGCMC	1 cut(s)	62
BseX3I	CGGCCG	1 cut(s)	124
BseXI	GCAGC	5 cut(s)	808, 1021, 1146, 1913, 2828
BseYI	CCCAGC	2 cut(s)	215, 1796
Bsh1285I	CGRYCG	1 cut(s)	127
BsiEI	CGRYCG	1 cut(s)	127
BsiHKCI	CYCGRG	1 cut(s)	2591
BsiSI	CCGG	9 cut(s)	42, 76, 123, 127, 151, 388, 1628, 2414, 2648
BslFI	GGGAC	3 cut(s)	232, 454, 1512
BsmAI	GTCTC	5 cut(s)	1037, 1385, 1827, 2055, 2468
BsmFI	GGGAC	3 cut(s)	232, 454, 1512
BsmI	GAATGC	1 cut(s)	2269
Bso31I	GGTCTC	1 cut(s)	2468
BsoBI	CYCGRG	1 cut(s)	2591
Bsp119I	TTCGAA	1 cut(s)	598
Bsp120I	GGGCCC	1 cut(s)	58
Bsp1286I	GDGCHC	2 cut(s)	49, 62
Bsp1407I	TGTACA	2 cut(s)	879, 1730
Bsp19I	CCATGG	1 cut(s)	2448
BspACI	CCGC	7 cut(s)	308, 371, 991, 1866, 2390, 2793, 2824
BspCNI	CTCAG	6 cut(s)	214, 220, 787, 1362, 2305, 2494
BspLI	GGNNCC	9 cut(s)	46, 60, 184, 357, 624, 1952, 2458, 2656, 2780
BspMAI	CTGCAG	2 cut(s)	2089, 2149
BspMI	ACCTGC	2 cut(s)	1931, 2092
BspOI	GCTAGC	1 cut(s)	371
BspPI	GGATC	8 cut(s)	350, 363, 440, 1619, 1649, 2400, 2773, 2786
BspQI	GCTCTTC	2 cut(s)	861, 1134
BspT104I	TTCGAA	1 cut(s)	598
BspTI	CTTAAG	1 cut(s)	718
BspTNI	GGTCTC	1 cut(s)	2468
BsrDI	GCAATG	4 cut(s)	302, 1824, 2712, 2754
BsrFI	RCCGGY	2 cut(s)	122, 2647
BsrGI	TGTACA	2 cut(s)	879, 1730
BsrI	ACTGG	8 cut(s)	1038, 1170, 2230, 2260, 2324, 2378, 2527, 2776
BssAI	RCCGGY	2 cut(s)	122, 2647
BssECI	CCNNGG	8 cut(s)	210, 326, 437, 559, 800, 1226, 1227, 2448
BssNI	GRCGYC	2 cut(s)	72, 1217
BssSI	CACGAG	1 cut(s)	28
BssT1I	CCWWGG	3 cut(s)	210, 559, 2448
Bst2BI	CACGAG	1 cut(s)	28
Bst2UI	CCWGG	5 cut(s)	327, 438, 1228, 1395, 1530
Bst4CI	ACNGT	9 cut(s)	53, 289, 518, 634, 1241, 1982, 2016, 2049, 2572
Bst6I	CTCTTC	4 cut(s)	22, 861, 960, 1134
BstACI	GRCGYC	2 cut(s)	72, 1217
BstAFI	CTTAAG	1 cut(s)	718
BstAPI	GCANNNNNTGC	3 cut(s)	1067, 1910, 2093
BstAUI	TGTACA	2 cut(s)	879, 1730
BstBAI	YACGTR	3 cut(s)	1196, 1433, 1856
BstBI	TTCGAA	1 cut(s)	598
BstDEI	CTNAG	7 cut(s)	222, 228, 234, 774, 1349, 2292, 2481
BstDSI	CCRYGG	1 cut(s)	2448
BstEII	GGTNACC	1 cut(s)	563
BstENI	CCTNNNNNAGG	1 cut(s)	1712
BstF5I	GGATG	4 cut(s)	436, 679, 2674, 2699
BstH2I	RGCGCY	1 cut(s)	2513
BstHHI	GCGC	1 cut(s)	2512
BstMAI	GTCTC	5 cut(s)	1037, 1385, 1827, 2055, 2468
BstMCI	CGRYCG	1 cut(s)	127
BstNI	CCWGG	5 cut(s)	327, 438, 1228, 1395, 1530
BstNSI	RCATGY	3 cut(s)	340, 1619, 1866
BstPI	GGTNACC	1 cut(s)	563
BstSCI	CCNGG	6 cut(s)	150, 325, 436, 1226, 1393, 1528
BstSFI	CTRYAG	6 cut(s)	35, 249, 1154, 2028, 2085, 2145
BstSLI	GKGCMC	1 cut(s)	62
BstV1I	GCAGC	5 cut(s)	808, 1021, 1146, 1913, 2828
BstV2I	GAAGAC	5 cut(s)	740, 1293, 1853, 2813, 2843
BstX2I	RGATCY	3 cut(s)	355, 1974, 2778
BstYI	RGATCY	3 cut(s)	355, 1974, 2778
BstZI	CGGCCG	1 cut(s)	124
BsuI	GTATCC	2 cut(s)	566, 2272
BtgI	CCRYGG	1 cut(s)	2448
BtgZI	GCGATG	1 cut(s)	1487
BtrI	CACGTC	1 cut(s)	877
BtsCI	GGATG	4 cut(s)	436, 679, 2674, 2699
BtsI	GCAGTG	1 cut(s)	854
BtsIMutI	CAGTG	3 cut(s)	854, 2568, 2962
BveI	ACCTGC	2 cut(s)	1931, 2092
CaiI	CAGNNNCTG	5 cut(s)	443, 467, 1162, 2102, 2255
CfoI	GCGC	1 cut(s)	2512
Cfr10I	RCCGGY	2 cut(s)	122, 2647
Cfr13I	GGNCC	7 cut(s)	58, 59, 78, 622, 1388, 2655, 2672
CseI	GACGC	1 cut(s)	61
Csp6I	GTAC	5 cut(s)	534, 880, 1731, 2045, 2553
CviQI	GTAC	5 cut(s)	534, 880, 1731, 2045, 2553
DdeI	CTNAG	7 cut(s)	222, 228, 234, 774, 1349, 2292, 2481
DraI	TTTAAA	1 cut(s)	891
DraIII	CACNNNGTG	1 cut(s)	2572
EaeI	YGGCCR	3 cut(s)	124, 134, 2574
EagI	CGGCCG	1 cut(s)	124
Eam1104I	CTCTTC	4 cut(s)	22, 861, 960, 1134
EarI	CTCTTC	4 cut(s)	22, 861, 960, 1134
EciI	GGCGGA	1 cut(s)	2839
EclXI	CGGCCG	1 cut(s)	124
Eco130I	CCWWGG	3 cut(s)	210, 559, 2448
Eco24I	GRGCYC	2 cut(s)	49, 62
Eco31I	GGTCTC	1 cut(s)	2468
Eco47I	GGWCC	1 cut(s)	622
Eco47III	AGCGCT	1 cut(s)	2511
Eco52I	CGGCCG	1 cut(s)	124
Eco57I	CTGAAG	5 cut(s)	228, 752, 923, 2256, 2874
Eco72I	CACGTG	2 cut(s)	1433, 1856
Eco88I	CYCGRG	1 cut(s)	2591
Eco91I	GGTNACC	1 cut(s)	563
EcoNI	CCTNNNNNAGG	1 cut(s)	1712
EcoO109I	RGGNCCY	2 cut(s)	59, 622
EcoO65I	GGTNACC	1 cut(s)	563
EcoRII	CCWGG	5 cut(s)	325, 436, 1226, 1393, 1528
EcoT14I	CCWWGG	3 cut(s)	210, 559, 2448
EcoT22I	ATGCAT	1 cut(s)	2271
EcoT38I	GRGCYC	2 cut(s)	49, 62
ErhI	CCWWGG	3 cut(s)	210, 559, 2448
FalI	AAGNNNNNCTT	8 cut(s)	561, 593, 1128, 1160, 2496, 2528, 2889, 2921
FaqI	GGGAC	3 cut(s)	232, 454, 1512
FauI	CCCGC	1 cut(s)	2786
FauNDI	CATATG	1 cut(s)	406
FbaI	TGATCA	1 cut(s)	2694
Fnu4HI	GCNGC	9 cut(s)	308, 372, 822, 991, 1010, 1160, 1867, 1902, 2817
FokI	GGATG	4 cut(s)	443, 686, 2681, 2686
FriOI	GRGCYC	2 cut(s)	49, 62
Fsp4HI	GCNGC	9 cut(s)	308, 372, 822, 991, 1010, 1160, 1867, 1902, 2817
GlaI	GCGC	1 cut(s)	2511
GluI	GCNGC	9 cut(s)	308, 372, 822, 991, 1010, 1160, 1867, 1902, 2817
GsaI	CCCAGC	2 cut(s)	219, 1800
GsuI	CTGGAG	4 cut(s)	432, 1184, 1416, 2247
HaeII	RGCGCY	1 cut(s)	2513
HapII	CCGG	9 cut(s)	42, 76, 123, 127, 151, 388, 1628, 2414, 2648
HgaI	GACGC	1 cut(s)	61
HhaI	GCGC	1 cut(s)	2512
Hin1I	GRCGYC	2 cut(s)	72, 1217
Hin6I	GCGC	1 cut(s)	2510
HinP1I	GCGC	1 cut(s)	2510
HincII	GTYRAC	3 cut(s)	643, 1215, 2528
HindII	GTYRAC	3 cut(s)	643, 1215, 2528
HindIII	AAGCTT	3 cut(s)	8, 935, 2630
HinfI	GANTC	9 cut(s)	130, 170, 1356, 1441, 2153, 2348, 2410, 2624, 2968
HpaII	CCGG	9 cut(s)	42, 76, 123, 127, 151, 388, 1628, 2414, 2648
HphI	GGTGA	9 cut(s)	112, 436, 575, 797, 922, 1634, 2270, 2321, 2372
Hpy166II	GTNNAC	8 cut(s)	258, 643, 880, 1112, 1215, 2261, 2528, 2914
Hpy8I	GTNNAC	8 cut(s)	258, 643, 880, 1112, 1215, 2261, 2528, 2914
Hpy99I	CGWCG	1 cut(s)	182
HpyAV	CCTTC	8 cut(s)	346, 421, 1021, 2486, 2731, 2792, 2860, 2894
HpyCH4III	ACNGT	9 cut(s)	53, 289, 518, 634, 1241, 1982, 2016, 2049, 2572
HpyCH4IV	ACGT	8 cut(s)	695, 876, 886, 1188, 1195, 1217, 1432, 1855
HpyF3I	CTNAG	7 cut(s)	222, 228, 234, 774, 1349, 2292, 2481
HpySE526I	ACGT	8 cut(s)	695, 876, 886, 1188, 1195, 1217, 1432, 1855
Hsp92I	GRCGYC	2 cut(s)	72, 1217
HspAI	GCGC	1 cut(s)	2510
KroI	GCCGGC	1 cut(s)	2647
KroNI	GCCGGC	1 cut(s)	2649
Ksp22I	TGATCA	1 cut(s)	2694
LguI	GCTCTTC	2 cut(s)	861, 1134
LmnI	GCTCC	9 cut(s)	44, 188, 406, 413, 818, 1645, 1765, 2228, 2507
Lsp1109I	GCAGC	5 cut(s)	808, 1021, 1146, 1913, 2828
LweI	GCATC	3 cut(s)	1878, 2023, 2406
MaeII	ACGT	8 cut(s)	695, 876, 886, 1188, 1195, 1217, 1432, 1855
MaeIII	GTNAC	8 cut(s)	96, 563, 785, 1189, 1891, 2206, 2360, 2761
MfeI	CAATTG	3 cut(s)	948, 1425, 2787
MflI	RGATCY	3 cut(s)	355, 1974, 2778
MhlI	GDGCHC	2 cut(s)	49, 62
MlsI	TGGCCA	1 cut(s)	2576
MluNI	TGGCCA	1 cut(s)	2576
MlyI	GAGTC	1 cut(s)	2404
MmeI	TCCRAC	6 cut(s)	183, 1321, 1447, 1540, 1623, 2398
Mox20I	TGGCCA	1 cut(s)	2576
Mph1103I	ATGCAT	1 cut(s)	2271
MroNI	GCCGGC	1 cut(s)	2647
MroXI	GAANNNNTTC	2 cut(s)	1275, 2612
MscI	TGGCCA	1 cut(s)	2576
MslI	CAYNNNNRTG	5 cut(s)	783, 1620, 2640, 2702, 2717
Msp20I	TGGCCA	1 cut(s)	2576
MspA1I	CMGCKG	1 cut(s)	1159
MspCI	CTTAAG	1 cut(s)	718
MspI	CCGG	9 cut(s)	42, 76, 123, 127, 151, 388, 1628, 2414, 2648
MspR9I	CCNGG	6 cut(s)	152, 327, 438, 1228, 1395, 1530
MunI	CAATTG	3 cut(s)	948, 1425, 2787
Mva1269I	GAATGC	1 cut(s)	2269
MvaI	CCWGG	5 cut(s)	327, 438, 1228, 1395, 1530
NaeI	GCCGGC	1 cut(s)	2649
NciI	CCSGG	1 cut(s)	152
NcoI	CCATGG	1 cut(s)	2448
NdeI	CATATG	1 cut(s)	406
NgoMIV	GCCGGC	1 cut(s)	2647
NheI	GCTAGC	1 cut(s)	367
NlaIV	GGNNCC	9 cut(s)	46, 60, 184, 357, 624, 1952, 2458, 2656, 2780
NmeAIII	GCCGAG	1 cut(s)	1958
NmuCI	GTSAC	6 cut(s)	563, 785, 1189, 1891, 2206, 2360
NsiI	ATGCAT	1 cut(s)	2271
NspI	RCATGY	3 cut(s)	340, 1619, 1866
NspV	TTCGAA	1 cut(s)	598
PaeI	GCATGC	1 cut(s)	1866
PaeR7I	CTCGAG	1 cut(s)	2591
PaqCI	CACCTGC	1 cut(s)	1931
PasI	CCCWGGG	1 cut(s)	1227
PciSI	GCTCTTC	2 cut(s)	861, 1134
PctI	GAATGC	1 cut(s)	2269
PdiI	GCCGGC	1 cut(s)	2649
PdmI	GAANNNNTTC	2 cut(s)	1275, 2612
PfeI	GAWTC	8 cut(s)	130, 170, 1356, 1441, 2153, 2348, 2624, 2968
PflMI	CCANNNNNTGG	4 cut(s)	55, 410, 2669, 2681
PfoI	TCCNGGA	1 cut(s)	1393
PkrI	GCNGC	9 cut(s)	309, 373, 823, 992, 1011, 1161, 1868, 1903, 2818
PleI	GAGTC	1 cut(s)	2404
PmaCI	CACGTG	2 cut(s)	1433, 1856
PmlI	CACGTG	2 cut(s)	1433, 1856
PpsI	GAGTC	1 cut(s)	2404
Ppu21I	YACGTR	3 cut(s)	1196, 1433, 1856
PpuMI	RGGWCCY	1 cut(s)	622
Psp1406I	AACGTT	1 cut(s)	886
Psp5II	RGGWCCY	1 cut(s)	622
Psp6I	CCWGG	5 cut(s)	325, 436, 1226, 1393, 1528
PspCI	CACGTG	2 cut(s)	1433, 1856
PspEI	GGTNACC	1 cut(s)	563
PspFI	CCCAGC	2 cut(s)	215, 1796
PspGI	CCWGG	5 cut(s)	325, 436, 1226, 1393, 1528
PspN4I	GGNNCC	9 cut(s)	46, 60, 184, 357, 624, 1952, 2458, 2656, 2780
PspOMI	GGGCCC	1 cut(s)	58
PspPI	GGNCC	7 cut(s)	58, 59, 78, 622, 1388, 2655, 2672
PspPPI	RGGWCCY	1 cut(s)	622
PstI	CTGCAG	2 cut(s)	2089, 2149
PstNI	CAGNNNCTG	5 cut(s)	443, 467, 1162, 2102, 2255
PsuI	RGATCY	3 cut(s)	355, 1974, 2778
PvuII	CAGCTG	1 cut(s)	1159
RsaI	GTAC	5 cut(s)	535, 881, 1732, 2046, 2554
RsaNI	GTAC	5 cut(s)	534, 880, 1731, 2045, 2553
RseI	CAYNNNNRTG	5 cut(s)	783, 1620, 2640, 2702, 2717
SapI	GCTCTTC	2 cut(s)	861, 1134
SatI	GCNGC	9 cut(s)	308, 372, 822, 991, 1010, 1160, 1867, 1902, 2817
Sau96I	GGNCC	7 cut(s)	58, 59, 78, 622, 1388, 2655, 2672
SchI	GAGTC	1 cut(s)	2404
ScrFI	CCNGG	6 cut(s)	152, 327, 438, 1228, 1395, 1530
SduI	GDGCHC	2 cut(s)	49, 62
SfaNI	GCATC	3 cut(s)	1878, 2023, 2406
SfcI	CTRYAG	6 cut(s)	35, 249, 1154, 2028, 2085, 2145
Sfr274I	CTCGAG	1 cut(s)	2591
SfuI	TTCGAA	1 cut(s)	598
SinI	GGWCC	1 cut(s)	622
SlaI	CTCGAG	1 cut(s)	2591
SmiMI	CAYNNNNRTG	5 cut(s)	783, 1620, 2640, 2702, 2717
SmlI	CTYRAG	3 cut(s)	650, 718, 2591
SmoI	CTYRAG	3 cut(s)	650, 718, 2591
SpeI	ACTAGT	1 cut(s)	737
SphI	GCATGC	1 cut(s)	1866
SsiI	CCGC	7 cut(s)	308, 371, 991, 1866, 2390, 2793, 2824
SspI	AATATT	1 cut(s)	2586
StyD4I	CCNGG	6 cut(s)	150, 325, 436, 1226, 1393, 1528
StyI	CCWWGG	3 cut(s)	210, 559, 2448
TaaI	ACNGT	9 cut(s)	53, 289, 518, 634, 1241, 1982, 2016, 2049, 2572
TaiI	ACGT	8 cut(s)	698, 879, 889, 1191, 1198, 1220, 1435, 1858
TaqI	TCGA	9 cut(s)	598, 1175, 1456, 1596, 1704, 1746, 1872, 2487, 2592
TatI	WGTACW	3 cut(s)	879, 1730, 2044
TauI	GCSGC	4 cut(s)	310, 374, 993, 1869
TfiI	GAWTC	8 cut(s)	130, 170, 1356, 1441, 2153, 2348, 2624, 2968
TscAI	CASTG	3 cut(s)	854, 2575, 2962
TseFI	GTSAC	6 cut(s)	563, 785, 1189, 1891, 2206, 2360
TseI	GCWGC	5 cut(s)	821, 1009, 1159, 1901, 2816
Tsp45I	GTSAC	6 cut(s)	563, 785, 1189, 1891, 2206, 2360
TspGWI	ACGGA	1 cut(s)	1311
TspRI	CASTG	3 cut(s)	854, 2575, 2962
Van91I	CCANNNNNTGG	4 cut(s)	55, 410, 2669, 2681
Vha464I	CTTAAG	1 cut(s)	718
VpaK11BI	GGWCC	1 cut(s)	622
XagI	CCTNNNNNAGG	1 cut(s)	1712
XapI	RAATTY	4 cut(s)	1181, 1275, 1328, 2298
XbaI	TCTAGA	1 cut(s)	1678
XceI	RCATGY	3 cut(s)	340, 1619, 1866
XhoI	CTCGAG	1 cut(s)	2591
XmnI	GAANNNNTTC	2 cut(s)	1275, 2612
ZraI	GACGTC	1 cut(s)	1218
Zsp2I	ATGCAT	1 cut(s)	2271

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.