Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003822

Physical Location & Seq

Genomic Coordinates Reverse (-)

13523403 .. 13525834

Gene Structure 2432 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G35330.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 819 bp

ATGAGTATGTCGCAGTCTCGAGCTGTCCAAAGATCGTCTTCACCTAATGAGGATAGAGGTGAGAATCAGTTAGTGGTTTATGACTTGAAAGGAAATGATGATACTGAAGAAGAAGTTCTACCGGTTCAATCTCAGCCTTTGTCATCTAGAACACAGTGTCCCTCTATCGGGGCATTTACGGTTCAGTGTGCAAGTTGTTTCAAATGGAGACTCATGCCTTCAATGCAGAAGTATGAAGAGATTAGGGAACAGCTTCTTGAAAACCCGTTTTTCTGCGATACAGCGCGTGAGTGGAAGCCGGATATTTCTTGTGATGTTCCTGCTGATATCTATCAAGATGGAACTCGTCTTTGGGCTATTGATAAACCGAATATCTCTCGTCCACCCGCTGGCTGGCAACGTTTACTGCGGATTAGAGGTGAAGGAGGTACCAGATTTGCTGATGTTTATTATGTTGCTCCGTCTGGAAAGAAGCTTCGATCAACTGTGGAGGTCCAAAAGTACTTGAATGACAACTCCGAATACATTGGAGAAGGGGTGAAACTTTCACAGTTCTCGTTTCAAATCCCGAAACCGCTGCAGGATGACTACGTGAGGAAGCGCCCAGCTCGACTACTGGATTCCATCGATAATACAAACACCCCTGTAGCTAAAGAAGCTAATCCACTGGCATGGATATCTCCAGATGATCATATATCGTTGCAGCTAGGTACGCCAACCGAGTCTGGACTTAACAATTCACATTACCAACCATCGAAAAAGAAAAAAACATCGACGCTATCAATCTTTGGCTCCAACGACGAACTTGCTGATAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0008327 GO:0019899 GO:0036094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MBD zf-CW

Protein Analysis

272

Amino Acids

30.77

Weight (kDa)

5.61

Isoelectric Point (pI)

57.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-CW	PF07496	60 - 109	1.3e-10	CW-type Zinc Finger
MBD	PF01429	123 - 189	7.7e-14	Methyl-CpG binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003822)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35330 AT5G35330 AT5G35330 AT5G35330
fragaria_vesca	FvH4_4g06240 FvH4_4g06240 FvH4_4g06240 FvH4_4g06240 FvH4_4g06240
malus_domestica	MD13G1234700.v1.1 MD16G1239500.v1.1
prunus_persica	Prupe.1G065600_v2.0.a1 Prupe.1G065600_v2.0.a1
pyrus_communis	pycom16g20040
rosa_chinensis	RchiOBHm_Chr4g0397961
rosa_laevigata	RLG00000009392
rosa_multiflora	Rmu_co8227835.1_g000001 Rmu_co8419633.1_g000001 Rmu_sc0001074.1_g000002 Rmu_sc0001074.1_g000005 Rmu_sc0003773.1_g000027
rosa_roxburghii	Rroxscaffold_5G00342870 Rroxscaffold_5G00342930
rosa_rugosa	Rorug04G0004900 Rorug04G0005000
rosa_samantha	Rh4AG081200 Rh4AG081600 Rh4BG079200 Rh4CG088800 Rh4CG089100 Rh4DG074700
rosa_wichuraiana	Rw0G019360 Rw4G006770 Rw4G006790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	428
AccB1I	GGYRCC	1 cut(s)	428
AccB7I	CCANNNNNTGG	1 cut(s)	389
AccII	CGCG	1 cut(s)	286
AciI	CCGC	3 cut(s)	387, 409, 575
AclI	AACGTT	1 cut(s)	400
AcuI	CTGAAG	1 cut(s)	126
AfaI	GTAC	3 cut(s)	430, 503, 712
AfiI	CCNNNNNNNGG	4 cut(s)	167, 168, 389, 393
AgeI	ACCGGT	1 cut(s)	121
AgsI	TTSAA	7 cut(s)	88, 128, 202, 222, 260, 508, 563
AjuI	GAANNNNNNNTTGG	2 cut(s)	334, 366
AloI	GAACNNNNNNTCC	2 cut(s)	142, 174
AluBI	AGCT	7 cut(s)	23, 253, 475, 608, 650, 659, 706
AluI	AGCT	7 cut(s)	23, 253, 475, 608, 650, 659, 706
Alw26I	GTCTC	2 cut(s)	21, 202
Ama87I	CYCGRG	1 cut(s)	18
ApeKI	GCWGC	2 cut(s)	577, 703
AsiGI	ACCGGT	1 cut(s)	121
Asp700I	GAANNNNTTC	2 cut(s)	114, 252
Asp718I	GGTACC	1 cut(s)	428
AspLEI	GCGC	2 cut(s)	286, 603
AspS9I	GGNCC	1 cut(s)	493
AsuHPI	GGTGA	4 cut(s)	33, 71, 431, 550
AvaI	CYCGRG	1 cut(s)	18
AvaII	GGWCC	1 cut(s)	493
BanI	GGYRCC	1 cut(s)	428
BarI	GAAGNNNNNNTAC	2 cut(s)	102, 134
BbsI	GAAGAC	1 cut(s)	30
BbvI	GCAGC	2 cut(s)	564, 715
BccI	CCATC	3 cut(s)	332, 632, 760
BcgI	CGANNNNNNTGC	3 cut(s)	559, 593, 788
BclI	TGATCA	1 cut(s)	688
BcoDI	GTCTC	2 cut(s)	21, 202
BfaI	CTAG	2 cut(s)	147, 707
BfmI	CTRYAG	2 cut(s)	578, 645
BfoI	RGCGCY	1 cut(s)	604
BisI	GCNGC	2 cut(s)	578, 704
BlsI	GCNGC	2 cut(s)	579, 705
BmcAI	AGTACT	1 cut(s)	503
Bme18I	GGWCC	1 cut(s)	493
BmeT110I	CYCGRG	1 cut(s)	18
BmgT120I	GGNCC	1 cut(s)	493
BmiI	GGNNCC	2 cut(s)	430, 793
BpiI	GAAGAC	1 cut(s)	30
BpmI	CTGGAG	1 cut(s)	666
Bsa29I	ATCGAT	1 cut(s)	627
BsaAI	YACGTR	1 cut(s)	592
BsaBI	GATNNNNATC	1 cut(s)	330
BsaWI	WCCGGW	1 cut(s)	121
BsaXI	ACNNNNNCTCC	2 cut(s)	522, 552
Bsc4I	CCNNNNNNNGG	4 cut(s)	167, 168, 389, 393
Bse118I	RCCGGY	1 cut(s)	121
Bse1I	ACTGG	2 cut(s)	621, 672
Bse8I	GATNNNNATC	1 cut(s)	330
BseCI	ATCGAT	1 cut(s)	627
BseGI	GGATG	1 cut(s)	589
BseJI	GATNNNNATC	1 cut(s)	330
BseLI	CCNNNNNNNGG	4 cut(s)	167, 168, 389, 393
BseMII	CTCAG	1 cut(s)	146
BseNI	ACTGG	2 cut(s)	621, 672
BseXI	GCAGC	2 cut(s)	564, 715
BseYI	CCCAGC	1 cut(s)	604
Bsh1236I	CGCG	1 cut(s)	286
BshNI	GGYRCC	1 cut(s)	428
BshTI	ACCGGT	1 cut(s)	121
BshVI	ATCGAT	1 cut(s)	627
BsiHKCI	CYCGRG	1 cut(s)	18
BsiSI	CCGG	2 cut(s)	122, 299
BslFI	GGGAC	1 cut(s)	144
BslI	CCNNNNNNNGG	4 cut(s)	167, 168, 389, 393
BsmAI	GTCTC	2 cut(s)	21, 202
BsmFI	GGGAC	1 cut(s)	144
BsoBI	CYCGRG	1 cut(s)	18
Bsp143I	GATC	3 cut(s)	32, 479, 688
BspACI	CCGC	3 cut(s)	387, 409, 575
BspCNI	CTCAG	1 cut(s)	145
BspDI	ATCGAT	1 cut(s)	627
BspFNI	CGCG	1 cut(s)	286
BspLI	GGNNCC	2 cut(s)	430, 793
BspMAI	CTGCAG	1 cut(s)	582
BspT107I	GGYRCC	1 cut(s)	428
BsrFI	RCCGGY	1 cut(s)	121
BsrI	ACTGG	2 cut(s)	621, 672
BssAI	RCCGGY	1 cut(s)	121
BssMI	GATC	3 cut(s)	32, 479, 688
Bst4CI	ACNGT	4 cut(s)	156, 181, 487, 552
Bst6I	CTCTTC	1 cut(s)	231
BstBAI	YACGTR	1 cut(s)	592
BstC8I	GCNNGC	2 cut(s)	391, 395
BstDEI	CTNAG	1 cut(s)	132
BstF5I	GGATG	1 cut(s)	589
BstFNI	CGCG	1 cut(s)	286
BstH2I	RGCGCY	1 cut(s)	604
BstHHI	GCGC	2 cut(s)	286, 603
BstKTI	GATC	3 cut(s)	35, 482, 691
BstMAI	GTCTC	2 cut(s)	21, 202
BstMBI	GATC	3 cut(s)	32, 479, 688
BstMWI	GCNNNNNNNGC	3 cut(s)	223, 656, 712
BstSFI	CTRYAG	2 cut(s)	578, 645
BstUI	CGCG	1 cut(s)	286
BstV1I	GCAGC	2 cut(s)	564, 715
BstV2I	GAAGAC	1 cut(s)	30
BstXI	CCANNNNNNTGG	1 cut(s)	672
Bsu15I	ATCGAT	1 cut(s)	627
BsuTUI	ATCGAT	1 cut(s)	627
BtsCI	GGATG	1 cut(s)	589
BtsIMutI	CAGTG	3 cut(s)	161, 191, 665
Cac8I	GCNNGC	2 cut(s)	391, 395
CfoI	GCGC	2 cut(s)	286, 603
Cfr10I	RCCGGY	1 cut(s)	121
Cfr13I	GGNCC	1 cut(s)	493
ClaI	ATCGAT	1 cut(s)	627
CseI	GACGC	1 cut(s)	784
Csp6I	GTAC	3 cut(s)	429, 502, 711
CspAI	ACCGGT	1 cut(s)	121
CviAII	CATG	2 cut(s)	214, 672
CviQI	GTAC	3 cut(s)	429, 502, 711
DdeI	CTNAG	1 cut(s)	132
DpnI	GATC	3 cut(s)	34, 481, 690
DpnII	GATC	3 cut(s)	32, 479, 688
Eam1104I	CTCTTC	1 cut(s)	231
EarI	CTCTTC	1 cut(s)	231
Eco32I	GATATC	2 cut(s)	328, 678
Eco47I	GGWCC	1 cut(s)	493
Eco57I	CTGAAG	1 cut(s)	126
Eco88I	CYCGRG	1 cut(s)	18
EcoRV	GATATC	2 cut(s)	328, 678
FaeI	CATG	2 cut(s)	217, 675
FaiI	YATR	8 cut(s)	8, 81, 215, 234, 453, 673, 693, 695
FalI	AAGNNNNNCTT	2 cut(s)	22, 54
FaqI	GGGAC	1 cut(s)	144
FatI	CATG	2 cut(s)	213, 671
FauI	CCCGC	1 cut(s)	394
FbaI	TGATCA	1 cut(s)	688
Fnu4HI	GCNGC	2 cut(s)	578, 704
FokI	GGATG	1 cut(s)	596
Fsp4HI	GCNGC	2 cut(s)	578, 704
FspBI	CTAG	2 cut(s)	147, 707
GlaI	GCGC	2 cut(s)	285, 602
GluI	GCNGC	2 cut(s)	578, 704
GsaI	CCCAGC	1 cut(s)	608
GsuI	CTGGAG	1 cut(s)	666
HaeII	RGCGCY	1 cut(s)	604
HapII	CCGG	2 cut(s)	122, 299
HgaI	GACGC	1 cut(s)	784
HhaI	GCGC	2 cut(s)	286, 603
Hin1II	CATG	2 cut(s)	217, 675
Hin6I	GCGC	2 cut(s)	284, 601
HinP1I	GCGC	2 cut(s)	284, 601
HindIII	AAGCTT	1 cut(s)	473
HinfI	GANTC	4 cut(s)	64, 210, 620, 722
HpaII	CCGG	2 cut(s)	122, 299
HphI	GGTGA	4 cut(s)	33, 71, 431, 550
Hpy166II	GTNNAC	2 cut(s)	383, 404
Hpy188I	TCNGA	1 cut(s)	520
Hpy188III	TCNNGA	8 cut(s)	18, 147, 257, 335, 465, 568, 683, 726
Hpy8I	GTNNAC	2 cut(s)	383, 404
Hpy99I	CGWCG	2 cut(s)	778, 803
HpyAV	CCTTC	3 cut(s)	228, 416, 527
HpyCH4III	ACNGT	4 cut(s)	156, 181, 487, 552
HpyCH4IV	ACGT	2 cut(s)	400, 591
HpyCH4V	TGCA	4 cut(s)	191, 226, 580, 703
HpyF10VI	GCNNNNNNNGC	3 cut(s)	223, 656, 712
HpyF3I	CTNAG	1 cut(s)	132
HpySE526I	ACGT	2 cut(s)	400, 591
Hsp92II	CATG	2 cut(s)	217, 675
HspAI	GCGC	2 cut(s)	284, 601
KpnI	GGTACC	1 cut(s)	432
Ksp22I	TGATCA	1 cut(s)	688
Kzo9I	GATC	3 cut(s)	32, 479, 688
LmnI	GCTCC	2 cut(s)	463, 797
Lsp1109I	GCAGC	2 cut(s)	564, 715
MaeI	CTAG	2 cut(s)	147, 707
MaeII	ACGT	2 cut(s)	400, 591
MalI	GATC	3 cut(s)	34, 481, 690
MboI	GATC	3 cut(s)	32, 479, 688
MboII	GAAGA	4 cut(s)	30, 119, 122, 248
MluCI	AATT	1 cut(s)	736
MlyI	GAGTC	2 cut(s)	204, 731
MmeI	TCCRAC	1 cut(s)	819
MnlI	CCTC	7 cut(s)	43, 50, 172, 410, 419, 484, 588
MroXI	GAANNNNTTC	2 cut(s)	114, 252
MseI	TTAA	1 cut(s)	732
MslI	CAYNNNNRTG	1 cut(s)	670
MspA1I	CMGCKG	2 cut(s)	389, 577
MspI	CCGG	2 cut(s)	122, 299
MvnI	CGCG	1 cut(s)	286
MwoI	GCNNNNNNNGC	3 cut(s)	223, 656, 712
NdeII	GATC	3 cut(s)	32, 479, 688
NlaIII	CATG	2 cut(s)	217, 675
NlaIV	GGNNCC	2 cut(s)	430, 793
PaeR7I	CTCGAG	1 cut(s)	18
PdmI	GAANNNNTTC	2 cut(s)	114, 252
PfeI	GAWTC	2 cut(s)	64, 620
PflMI	CCANNNNNTGG	1 cut(s)	389
PinAI	ACCGGT	1 cut(s)	121
PkrI	GCNGC	2 cut(s)	579, 705
PleI	GAGTC	2 cut(s)	204, 730
PpsI	GAGTC	2 cut(s)	204, 730
Ppu21I	YACGTR	1 cut(s)	592
Psp1406I	AACGTT	1 cut(s)	400
PspFI	CCCAGC	1 cut(s)	604
PspN4I	GGNNCC	2 cut(s)	430, 793
PspPI	GGNCC	1 cut(s)	493
PstI	CTGCAG	1 cut(s)	582
RsaI	GTAC	3 cut(s)	430, 503, 712
RsaNI	GTAC	3 cut(s)	429, 502, 711
RseI	CAYNNNNRTG	1 cut(s)	670
SaqAI	TTAA	1 cut(s)	732
SatI	GCNGC	2 cut(s)	578, 704
Sau3AI	GATC	3 cut(s)	32, 479, 688
Sau96I	GGNCC	1 cut(s)	493
ScaI	AGTACT	1 cut(s)	503
SchI	GAGTC	2 cut(s)	204, 731
SfcI	CTRYAG	2 cut(s)	578, 645
Sfr274I	CTCGAG	1 cut(s)	18
SinI	GGWCC	1 cut(s)	493
SlaI	CTCGAG	1 cut(s)	18
SmiMI	CAYNNNNRTG	1 cut(s)	670
SmlI	CTYRAG	1 cut(s)	18
SmoI	CTYRAG	1 cut(s)	18
Sse9I	AATT	1 cut(s)	736
SsiI	CCGC	3 cut(s)	387, 409, 575
SspMI	CTAG	2 cut(s)	147, 707
TaaI	ACNGT	4 cut(s)	156, 181, 487, 552
TaiI	ACGT	2 cut(s)	403, 594
TaqI	TCGA	6 cut(s)	19, 478, 610, 627, 755, 773
TasI	AATT	1 cut(s)	736
TatI	WGTACW	1 cut(s)	501
TfiI	GAWTC	2 cut(s)	64, 620
Tru1I	TTAA	1 cut(s)	732
Tru9I	TTAA	1 cut(s)	732
TscAI	CASTG	3 cut(s)	161, 191, 672
TseI	GCWGC	2 cut(s)	577, 703
TspDTI	ATGAA	1 cut(s)	249
TspGWI	ACGGA	1 cut(s)	450
TspRI	CASTG	3 cut(s)	161, 191, 672
Van91I	CCANNNNNTGG	1 cut(s)	389
VpaK11BI	GGWCC	1 cut(s)	493
XbaI	TCTAGA	1 cut(s)	146
XcmI	CCANNNNNNNNNTGG	1 cut(s)	390
XhoI	CTCGAG	1 cut(s)	18
XmnI	GAANNNNTTC	2 cut(s)	114, 252
XspI	CTAG	2 cut(s)	147, 707
ZrmI	AGTACT	1 cut(s)	503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.