Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0003822

Physical Location & Seq

Genomic Coordinates Reverse (-)

13892084 .. 13943641

Gene Structure 51558 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG079200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGGAATCCTCGACTCCGAGCCCTTGTAAGATCACCCTCAAGCTCAGAAGAGACGACGAACAAGAACAAGAGGCGAAAACGAACGACGACAAACCCGCCCAGTCTCCTTCGTCTTCCTCCGTCTCGTCGCCGCCGTCTTCCCCTTCCTCCTCCGACGAGATTCTCCAGTCCCATCTTCAATCCCAGGAGCTCGTGCTCTACGATCCCGCCGTCGCCGCTAATGCCGGTGGTGACGCCAATCGCGGCGCTGCTACAGTGAAGCGGATTCCGAATCTGAACAAATCCATTTCTCCGGTCTCGGTTTCGAATAGTCAAGGTCCTAGGGTTTTGCCGTCGGTCGGGGCCTTCACTGTGCAGTGCGCCAATTGCTTCAAGTGGAGGCTGATTCCGACGAAGGAGCAGTACGAGCAAATCCGGGAGCACATTCTGGAACGGCCGTTCTACTGTGAAACTGCAAAGGAATGGCGGCCGTCGATTTCGTGCGATGATCCGGCTGACATTACTCAAGACGGGAGCCGGCTCTGGGCCATTGATAGGCCCAACATTGCTCAGCCACCTCCCGGCTGGCAACGGCTACTCAGGATTCGAGGTGAAGGAAGCACCAAGTTTGCAGATGTTTATTATCAAGCGCCGTCGGGCAAGAGACTTCGGTCCATGGTGGAGGTTCAAAAGTATTTGATTGAACATCCTGAGTATATGGTGGATGGTCTATCTATGGCACAATTTTCATTTCAAATTCCAAAGCCTGTACAGGAGAAATATGTGAGGAAACGCCCTGCGGCTCCTCGGTTGACAGCAGCTTCCCATGCCAGTAGAACTCTTGAACCTACTGAAGCAAATCCCATAGCAAGGGCTGGTCCAGATGATATAGAGTTGCTACTTGGTTCTCCGTACTTGGAGGCTCATGTGTTTGACCCTGTTGGTCGACCTACAAAGAAGCGAGCCAAAACAAGAACCTCCATCAAGGGTCTCATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0008327 GO:0019899 GO:0036094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MBD zf-CW

Protein Analysis

325

Amino Acids

36.02

Weight (kDa)

8.86

Isoelectric Point (pI)

68.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-CW	PF07496	117 - 166	3.3e-11	CW-type Zinc Finger
MBD	PF01429	186 - 249	7.6e-14	Methyl-CpG binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003822)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35330 AT5G35330 AT5G35330 AT5G35330
fragaria_vesca	FvH4_4g06240 FvH4_4g06240 FvH4_4g06240 FvH4_4g06240 FvH4_4g06240
malus_domestica	MD13G1234700.v1.1 MD16G1239500.v1.1
prunus_persica	Prupe.1G065600_v2.0.a1 Prupe.1G065600_v2.0.a1
pyrus_communis	pycom16g20040
rosa_chinensis	RchiOBHm_Chr4g0397961
rosa_laevigata	RLG00000009392
rosa_multiflora	Rmu_co8227835.1_g000001 Rmu_co8419633.1_g000001 Rmu_sc0001074.1_g000002 Rmu_sc0001074.1_g000005 Rmu_sc0003773.1_g000027
rosa_roxburghii	Rroxscaffold_5G00342870 Rroxscaffold_5G00342930
rosa_rugosa	Rorug04G0004900 Rorug04G0005000
rosa_samantha	Rh4AG081200 Rh4AG081600 Rh4BG079200 Rh4CG088800 Rh4CG089100 Rh4DG074700
rosa_wichuraiana	Rw0G019360 Rw4G006770 Rw4G006790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	925
AccII	CGCG	1 cut(s)	243
AciI	CCGC	8 cut(s)	96, 131, 207, 216, 243, 262, 466, 779
AclWI	GGATC	2 cut(s)	197, 482
AcoI	YGGCCR	2 cut(s)	434, 467
AcsI	RAATTY	1 cut(s)	735
AcuI	CTGAAG	1 cut(s)	852
AcyI	GRCGYC	1 cut(s)	234
AfaI	GTAC	3 cut(s)	404, 750, 893
AfiI	CCNNNNNNNGG	5 cut(s)	338, 523, 534, 560, 849
AgsI	TTSAA	6 cut(s)	179, 373, 668, 683, 734, 824
AjnI	CCWGG	1 cut(s)	183
AloI	GAACNNNNNNTCC	2 cut(s)	422, 454
AluBI	AGCT	3 cut(s)	43, 190, 800
AluI	AGCT	3 cut(s)	43, 190, 800
Alw21I	GWGCWC	3 cut(s)	192, 198, 423
Alw26I	GTCTC	6 cut(s)	45, 108, 127, 301, 637, 974
AlwI	GGATC	2 cut(s)	197, 482
AoxI	GGCC	5 cut(s)	342, 434, 467, 525, 536
ApeKI	GCWGC	2 cut(s)	248, 797
ApoI	RAATTY	1 cut(s)	735
AspA2I	CCTAGG	1 cut(s)	320
AspLEI	GCGC	3 cut(s)	248, 362, 631
AspS9I	GGNCC	6 cut(s)	317, 342, 525, 537, 651, 857
AsuC2I	CCSGG	2 cut(s)	416, 561
AsuHPI	GGTGA	3 cut(s)	25, 242, 602
AsuII	TTCGAA	1 cut(s)	305
AvaII	GGWCC	3 cut(s)	317, 651, 857
AvrII	CCTAGG	1 cut(s)	320
BanII	GRGCYC	2 cut(s)	23, 192
BarI	GAAGNNNNNNTAC	2 cut(s)	386, 418
BauI	CACGAG	1 cut(s)	191
BbsI	GAAGAC	2 cut(s)	105, 129
Bbv12I	GWGCWC	3 cut(s)	192, 198, 423
BbvI	GCAGC	2 cut(s)	235, 809
BccI	CCATC	3 cut(s)	180, 698, 968
BceAI	ACGGC	8 cut(s)	118, 194, 316, 421, 449, 454, 587, 616
BciT130I	CCWGG	1 cut(s)	185
BcnI	CCSGG	2 cut(s)	416, 561
BcoDI	GTCTC	6 cut(s)	45, 108, 127, 301, 637, 974
BfaI	CTAG	1 cut(s)	321
BfmI	CTRYAG	1 cut(s)	252
BfoI	RGCGCY	2 cut(s)	249, 632
BisI	GCNGC	7 cut(s)	131, 216, 244, 249, 467, 780, 798
BlnI	CCTAGG	1 cut(s)	320
BlpI	GCTNAGC	1 cut(s)	549
BlsI	GCNGC	7 cut(s)	132, 217, 245, 250, 468, 781, 799
Bme1390I	CCNGG	3 cut(s)	185, 416, 561
Bme18I	GGWCC	3 cut(s)	317, 651, 857
BmgT120I	GGNCC	6 cut(s)	317, 342, 525, 537, 651, 857
BmiI	GGNNCC	3 cut(s)	343, 515, 783
BmrFI	CCNGG	3 cut(s)	185, 416, 561
BmrI	ACTGGG	1 cut(s)	94
BmuI	ACTGGG	1 cut(s)	94
BoxI	GACNNNNGTC	1 cut(s)	649
BpiI	GAAGAC	2 cut(s)	105, 129
BpmI	CTGGAG	1 cut(s)	149
Bpu1102I	GCTNAGC	1 cut(s)	549
Bpu14I	TTCGAA	1 cut(s)	305
BpuEI	CTTGAG	2 cut(s)	23, 489
BpuMI	CCSGG	2 cut(s)	416, 561
BsaHI	GRCGYC	1 cut(s)	234
BsaI	GGTCTC	2 cut(s)	301, 974
BsaJI	CCNNGG	4 cut(s)	183, 320, 654, 785
BsaWI	WCCGGW	1 cut(s)	292
Bsc4I	CCNNNNNNNGG	5 cut(s)	338, 523, 534, 560, 849
Bse118I	RCCGGY	2 cut(s)	224, 516
Bse1I	ACTGG	3 cut(s)	100, 166, 810
Bse3DI	GCAATG	1 cut(s)	543
BseBI	CCWGG	1 cut(s)	185
BseDI	CCNNGG	4 cut(s)	183, 320, 654, 785
BseGI	GGATG	2 cut(s)	685, 709
BseLI	CCNNNNNNNGG	5 cut(s)	338, 523, 534, 560, 849
BseMI	GCAATG	1 cut(s)	543
BseMII	CTCAG	4 cut(s)	58, 563, 592, 681
BseNI	ACTGG	3 cut(s)	100, 166, 810
BseRI	GAGGAG	2 cut(s)	139, 774
BseX3I	CGGCCG	2 cut(s)	434, 467
BseXI	GCAGC	2 cut(s)	235, 809
BsgI	GTGCAG	1 cut(s)	374
Bsh1236I	CGCG	1 cut(s)	243
Bsh1285I	CGRYCG	3 cut(s)	339, 437, 470
BshFI	GGCC	5 cut(s)	344, 436, 469, 527, 538
BsiEI	CGRYCG	3 cut(s)	339, 437, 470
BsiHKAI	GWGCWC	3 cut(s)	192, 198, 423
BsiSI	CCGG	6 cut(s)	225, 293, 415, 491, 517, 561
BslFI	GGGAC	1 cut(s)	154
BslI	CCNNNNNNNGG	5 cut(s)	338, 523, 534, 560, 849
BsmAI	GTCTC	6 cut(s)	45, 108, 127, 301, 637, 974
BsmBI	CGTCTC	2 cut(s)	45, 127
BsmFI	GGGAC	1 cut(s)	154
BsnI	GGCC	5 cut(s)	344, 436, 469, 527, 538
Bso31I	GGTCTC	2 cut(s)	301, 974
Bsp119I	TTCGAA	1 cut(s)	305
Bsp1286I	GDGCHC	4 cut(s)	23, 192, 198, 423
Bsp1407I	TGTACA	1 cut(s)	748
Bsp143I	GATC	3 cut(s)	30, 202, 487
Bsp1720I	GCTNAGC	1 cut(s)	549
Bsp19I	CCATGG	1 cut(s)	654
BspACI	CCGC	8 cut(s)	96, 131, 207, 216, 243, 262, 466, 779
BspANI	GGCC	5 cut(s)	344, 436, 469, 527, 538
BspCNI	CTCAG	4 cut(s)	57, 562, 591, 682
BspFNI	CGCG	1 cut(s)	243
BspLI	GGNNCC	3 cut(s)	343, 515, 783
BspPI	GGATC	2 cut(s)	197, 482
BspT104I	TTCGAA	1 cut(s)	305
BspTNI	GGTCTC	2 cut(s)	301, 974
BsrDI	GCAATG	1 cut(s)	543
BsrFI	RCCGGY	2 cut(s)	224, 516
BsrGI	TGTACA	1 cut(s)	748
BsrI	ACTGG	3 cut(s)	100, 166, 810
BssAI	RCCGGY	2 cut(s)	224, 516
BssECI	CCNNGG	4 cut(s)	183, 320, 654, 785
BssMI	GATC	3 cut(s)	30, 202, 487
BssNI	GRCGYC	1 cut(s)	234
BssSI	CACGAG	1 cut(s)	191
BssT1I	CCWWGG	2 cut(s)	320, 654
Bst2BI	CACGAG	1 cut(s)	191
Bst2UI	CCWGG	1 cut(s)	185
Bst4CI	ACNGT	3 cut(s)	256, 352, 446
Bst6I	CTCTTC	1 cut(s)	43
BstACI	GRCGYC	1 cut(s)	234
BstAUI	TGTACA	1 cut(s)	748
BstBI	TTCGAA	1 cut(s)	305
BstC8I	GCNNGC	3 cut(s)	518, 566, 942
BstDEI	CTNAG	4 cut(s)	44, 549, 578, 690
BstDSI	CCRYGG	1 cut(s)	654
BstF5I	GGATG	2 cut(s)	685, 709
BstFNI	CGCG	1 cut(s)	243
BstH2I	RGCGCY	2 cut(s)	249, 632
BstHHI	GCGC	3 cut(s)	248, 362, 631
BstKTI	GATC	3 cut(s)	33, 205, 490
BstMAI	GTCTC	6 cut(s)	45, 108, 127, 301, 637, 974
BstMBI	GATC	3 cut(s)	30, 202, 487
BstMCI	CGRYCG	3 cut(s)	339, 437, 470
BstMWI	GCNNNNNNNGC	5 cut(s)	215, 221, 366, 406, 806
BstNI	CCWGG	1 cut(s)	185
BstPAI	GACNNNNGTC	1 cut(s)	649
BstSCI	CCNGG	3 cut(s)	183, 414, 559
BstSFI	CTRYAG	1 cut(s)	252
BstUI	CGCG	1 cut(s)	243
BstV1I	GCAGC	2 cut(s)	235, 809
BstV2I	GAAGAC	2 cut(s)	105, 129
BstZI	CGGCCG	2 cut(s)	434, 467
BsuRI	GGCC	5 cut(s)	344, 436, 469, 527, 538
BtgI	CCRYGG	1 cut(s)	654
BtgZI	GCGATG	1 cut(s)	498
BtsCI	GGATG	2 cut(s)	685, 709
BtsI	GCAGTG	1 cut(s)	362
BtsIMutI	CAGTG	3 cut(s)	261, 348, 362
Cac8I	GCNNGC	3 cut(s)	518, 566, 942
CfoI	GCGC	3 cut(s)	248, 362, 631
Cfr10I	RCCGGY	2 cut(s)	224, 516
Cfr13I	GGNCC	6 cut(s)	317, 342, 525, 537, 651, 857
CseI	GACGC	1 cut(s)	242
Csp6I	GTAC	3 cut(s)	403, 749, 892
CviAII	CATG	3 cut(s)	655, 806, 905
CviQI	GTAC	3 cut(s)	403, 749, 892
DdeI	CTNAG	4 cut(s)	44, 549, 578, 690
DpnI	GATC	3 cut(s)	32, 204, 489
DpnII	GATC	3 cut(s)	30, 202, 487
EaeI	YGGCCR	2 cut(s)	434, 467
EagI	CGGCCG	2 cut(s)	434, 467
Eam1104I	CTCTTC	1 cut(s)	43
EarI	CTCTTC	1 cut(s)	43
Ecl136II	GAGCTC	1 cut(s)	190
EclXI	CGGCCG	2 cut(s)	434, 467
Eco130I	CCWWGG	2 cut(s)	320, 654
Eco24I	GRGCYC	2 cut(s)	23, 192
Eco31I	GGTCTC	2 cut(s)	301, 974
Eco47I	GGWCC	3 cut(s)	317, 651, 857
Eco52I	CGGCCG	2 cut(s)	434, 467
Eco53kI	GAGCTC	1 cut(s)	190
Eco57I	CTGAAG	1 cut(s)	852
EcoICRI	GAGCTC	1 cut(s)	190
EcoO109I	RGGNCCY	2 cut(s)	317, 342
EcoRII	CCWGG	1 cut(s)	183
EcoT14I	CCWWGG	2 cut(s)	320, 654
EcoT38I	GRGCYC	2 cut(s)	23, 192
ErhI	CCWWGG	2 cut(s)	320, 654
Esp3I	CGTCTC	2 cut(s)	45, 127
FaeI	CATG	3 cut(s)	658, 809, 908
FaiI	YATR	9 cut(s)	656, 696, 698, 716, 762, 807, 845, 869, 906
FaqI	GGGAC	1 cut(s)	154
FatI	CATG	3 cut(s)	654, 805, 904
FauI	CCCGC	2 cut(s)	103, 214
FblI	GTMKAC	1 cut(s)	925
Fnu4HI	GCNGC	7 cut(s)	131, 216, 244, 249, 467, 780, 798
FokI	GGATG	2 cut(s)	672, 716
FriOI	GRGCYC	2 cut(s)	23, 192
Fsp4HI	GCNGC	7 cut(s)	131, 216, 244, 249, 467, 780, 798
FspBI	CTAG	1 cut(s)	321
GlaI	GCGC	3 cut(s)	247, 361, 630
GluI	GCNGC	7 cut(s)	131, 216, 244, 249, 467, 780, 798
GsuI	CTGGAG	1 cut(s)	149
HaeII	RGCGCY	2 cut(s)	249, 632
HaeIII	GGCC	5 cut(s)	344, 436, 469, 527, 538
HapII	CCGG	6 cut(s)	225, 293, 415, 491, 517, 561
HgaI	GACGC	1 cut(s)	242
HhaI	GCGC	3 cut(s)	248, 362, 631
Hin1I	GRCGYC	1 cut(s)	234
Hin1II	CATG	3 cut(s)	658, 809, 908
Hin6I	GCGC	3 cut(s)	246, 360, 629
HinP1I	GCGC	3 cut(s)	246, 360, 629
HincII	GTYRAC	2 cut(s)	792, 926
HindII	GTYRAC	2 cut(s)	792, 926
HinfI	GANTC	7 cut(s)	5, 13, 160, 265, 271, 385, 583
HpaII	CCGG	6 cut(s)	225, 293, 415, 491, 517, 561
HphI	GGTGA	3 cut(s)	25, 242, 602
Hpy166II	GTNNAC	2 cut(s)	792, 926
Hpy188I	TCNGA	6 cut(s)	18, 47, 154, 270, 276, 390
Hpy188III	TCNNGA	6 cut(s)	428, 506, 580, 689, 821, 860
Hpy8I	GTNNAC	2 cut(s)	792, 926
Hpy99I	CGWCG	9 cut(s)	59, 89, 130, 158, 215, 337, 394, 475, 637
HpyAV	CCTTC	5 cut(s)	117, 153, 355, 388, 587
HpyCH4III	ACNGT	3 cut(s)	256, 352, 446
HpyCH4V	TGCA	3 cut(s)	355, 455, 611
HpyF10VI	GCNNNNNNNGC	5 cut(s)	215, 221, 366, 406, 806
HpyF3I	CTNAG	4 cut(s)	44, 549, 578, 690
Hsp92I	GRCGYC	1 cut(s)	234
Hsp92II	CATG	3 cut(s)	658, 809, 908
HspAI	GCGC	3 cut(s)	246, 360, 629
KroI	GCCGGC	1 cut(s)	516
KroNI	GCCGGC	1 cut(s)	518
Kzo9I	GATC	3 cut(s)	30, 202, 487
LmnI	GCTCC	5 cut(s)	187, 397, 418, 513, 787
Lsp1109I	GCAGC	2 cut(s)	235, 809
MaeI	CTAG	1 cut(s)	321
MaeIII	GTNAC	1 cut(s)	230
MalI	GATC	3 cut(s)	32, 204, 489
MboI	GATC	3 cut(s)	30, 202, 487
MboII	GAAGA	4 cut(s)	60, 105, 129, 167
MfeI	CAATTG	1 cut(s)	364
MhlI	GDGCHC	4 cut(s)	23, 192, 198, 423
MluCI	AATT	3 cut(s)	364, 722, 735
MlyI	GAGTC	1 cut(s)	7
MmeI	TCCRAC	2 cut(s)	177, 413
MroNI	GCCGGC	1 cut(s)	516
MseI	TTAA	1 cut(s)	976
MspI	CCGG	6 cut(s)	225, 293, 415, 491, 517, 561
MspR9I	CCNGG	3 cut(s)	185, 416, 561
MunI	CAATTG	1 cut(s)	364
MvaI	CCWGG	1 cut(s)	185
MvnI	CGCG	1 cut(s)	243
MwoI	GCNNNNNNNGC	5 cut(s)	215, 221, 366, 406, 806
NaeI	GCCGGC	1 cut(s)	518
NciI	CCSGG	2 cut(s)	416, 561
NcoI	CCATGG	1 cut(s)	654
NdeII	GATC	3 cut(s)	30, 202, 487
NgoMIV	GCCGGC	1 cut(s)	516
NlaIII	CATG	3 cut(s)	658, 809, 908
NlaIV	GGNNCC	3 cut(s)	343, 515, 783
NmuCI	GTSAC	1 cut(s)	230
NspV	TTCGAA	1 cut(s)	305
PcsI	WCGNNNNNNNCGW	3 cut(s)	131, 198, 207
PdiI	GCCGGC	1 cut(s)	518
PfeI	GAWTC	6 cut(s)	5, 160, 265, 271, 385, 583
PfoI	TCCNGGA	1 cut(s)	414
PkrI	GCNGC	7 cut(s)	132, 217, 245, 250, 468, 781, 799
PleI	GAGTC	1 cut(s)	7
PpsI	GAGTC	1 cut(s)	7
PpuMI	RGGWCCY	1 cut(s)	317
PshAI	GACNNNNGTC	1 cut(s)	649
Psp124BI	GAGCTC	1 cut(s)	192
Psp5II	RGGWCCY	1 cut(s)	317
Psp6I	CCWGG	1 cut(s)	183
PspGI	CCWGG	1 cut(s)	183
PspN4I	GGNNCC	3 cut(s)	343, 515, 783
PspPI	GGNCC	6 cut(s)	317, 342, 525, 537, 651, 857
PspPPI	RGGWCCY	1 cut(s)	317
RsaI	GTAC	3 cut(s)	404, 750, 893
RsaNI	GTAC	3 cut(s)	403, 749, 892
SacI	GAGCTC	1 cut(s)	192
SalI	GTCGAC	1 cut(s)	924
SaqAI	TTAA	1 cut(s)	976
SatI	GCNGC	7 cut(s)	131, 216, 244, 249, 467, 780, 798
Sau3AI	GATC	3 cut(s)	30, 202, 487
Sau96I	GGNCC	6 cut(s)	317, 342, 525, 537, 651, 857
SchI	GAGTC	1 cut(s)	7
ScrFI	CCNGG	3 cut(s)	185, 416, 561
SduI	GDGCHC	4 cut(s)	23, 192, 198, 423
SfcI	CTRYAG	1 cut(s)	252
SfuI	TTCGAA	1 cut(s)	305
SinI	GGWCC	3 cut(s)	317, 651, 857
SmlI	CTYRAG	2 cut(s)	38, 504
SmoI	CTYRAG	2 cut(s)	38, 504
Sse9I	AATT	3 cut(s)	364, 722, 735
SsiI	CCGC	8 cut(s)	96, 131, 207, 216, 243, 262, 466, 779
SspMI	CTAG	1 cut(s)	321
SstI	GAGCTC	1 cut(s)	192
StyD4I	CCNGG	3 cut(s)	183, 414, 559
StyI	CCWWGG	2 cut(s)	320, 654
TaaI	ACNGT	3 cut(s)	256, 352, 446
TaqI	TCGA	5 cut(s)	11, 305, 473, 586, 925
TaqII	GACCGA	2 cut(s)	325, 639
TasI	AATT	3 cut(s)	364, 722, 735
TatI	WGTACW	1 cut(s)	748
TauI	GCSGC	5 cut(s)	133, 218, 246, 469, 782
TfiI	GAWTC	6 cut(s)	5, 160, 265, 271, 385, 583
Tru1I	TTAA	1 cut(s)	976
Tru9I	TTAA	1 cut(s)	976
TscAI	CASTG	3 cut(s)	261, 355, 362
TseFI	GTSAC	1 cut(s)	230
TseI	GCWGC	2 cut(s)	248, 797
Tsp45I	GTSAC	1 cut(s)	230
TspDTI	ATGAA	1 cut(s)	717
TspGWI	ACGGA	2 cut(s)	109, 879
TspRI	CASTG	3 cut(s)	261, 355, 362
VpaK11BI	GGWCC	3 cut(s)	317, 651, 857
XapI	RAATTY	1 cut(s)	735
XcmI	CCANNNNNNNNNTGG	1 cut(s)	561
XmaJI	CCTAGG	1 cut(s)	320
XmiI	GTMKAC	1 cut(s)	925
XspI	CTAG	1 cut(s)	321

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.