AT5G45400

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009053
Physical Location & Seq
Forward (+)
18398832 .. 18402284
3453 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G45400.1

Sequence Viewer

Length: 2562 bp
ATGGCGGTGAGTTTGACGGAGGGAGTGGTAATGAAGATGTTGAACGGAGAAGTGACGAGCGAGACAGATATGATGCCAGTGCTACAAGTAACGGAATTGAAGCTGATTCAGAGCAAGCTTCATCAAAATCAGGAATCTAGCAATCGATATAAGTTTTTATTATCAGATGGGACTGACTTGGCAGCAGGAATGCTTAATACTTCATTGAACAGTTTAGTGAATCAAGGAACTATTCAATTGGGTTCGGTTATAAGACTCACTCACTACATCTGCAATCTCATCCAGACTCGAAGGATTGTTGTTATTATGCAATTGGAGGTTATTGTGGAGAAATGTAATATAATTGGAAATCCTAAAGAACCTGGGCATAGCTCGATTAATCCACAACGTGGTGGAGTTAACACACAAAGCAATGGTGGTTCTGAGCAGCAACAAGCTAGAAGGAGTGATGTGAATGGTGGGAGATACGGTGTATCGGCTAATTCGCCTCAACCTCAAGTTGTTCATAACAGTTCCGACGCTGGAAGATACTGTGTATCGGCTAATTCACCTCAGCCTCAAGTTGTTCATAGCAGTTCCGACGCTGGAAGATATGGTGTATCGGCTAATTCACCTCAGCGTCAAGTTGTTCATAACAGTCCCGACGCTGGGAGATATGGACAGCCTCAAGTTAGTCAAAGATATGGTACTGGTTCTGGTTATCCGGAAACATCACCTTCCACTAGACCGTATGTGAGCTCAAATGCAGGTTATGGAGGCTCTAGGCAGGACCAACCTCGGGCTCCTACTGCAACAACTGCTTATTCCCGTCCGGTTCAGTCAGCTTATCAGCCACAACAACCACCGATGTATGTTAACAGGGGACCTGTGGCTAGGAACGAAGCTCCTCCGAGGATAAATCCGATTGCTGCTCTTAATCCTTACCAGGGTCGGTGGACCATCAAGGTTAGGGTCACGAGTAAGGCAGACCTGAGGCGGTTTAATAATCCGCGTGGGGAAGGGAAGTTGTTCAGTTTTGATCTTCTTGATGCTGATGGTGGAGAAATACGGGTGACTTGTTTTAATGATGCGGTTGATCAATTCTTTGACAAGATTGTAGTTGGTAATGTTTATCTGATTTCAAGGGGAAATTTGAAGCCTGCTCAGAAGAACTTTAATCATCTTCCGAATGATTATGAAATACATTTGGACTCTGCATCAACGATACAGCCTTGTGAAGATGATGGTACCATTCCACGCTATCATTTCCATTTTCGTAACATAGGTGATATTGAAAACATGGAGAACAACAGCACCACTGATGTAATTGGTATTGTTTCGTCCATCAGTCCGACAGTAGCAATTATGCGGAAAAACTTAACTGAGGTACAGAAAAGGTCTCTTCAGTTGAAGGATATGTCAGGCCGAAGTGTTGAGGTAACCATGTGGGGTAATTTCTGCAACGCAGAAGGACAGAAATTGCAAAATCTTTGTGATTCTGGGGTATTCCCGGTTCTTGCTTTGAAAGCGGGCAGGATCGGTGAATTCAACGGGAAGCAAGTGAGCACAATTGGAGCAAGTCAATTCTTTATAGAGCCAGATTTCCCTGAAGCCAGAGAATTAAGGCAATGGTATGAGAGAGAAGGAAGGAATGCTCATTTTACCTCCATATCTAGAGAGTTTTCCGGTGTTGGCAGACAAGAAGTTCGCAAGGTAATTGCACAGATCAAGGACGAGAAGCTAGGGACCTCAGAGAAGCCGGACTGGATCACAGTCTGTGCCACCATTTCATTCATGAAGGTTGAAAATTTCTGCTATACGGCTTGTCCTATCATGAATGGAGATCGTCCATGCAGCAAAAAGGTCACAAATAACGGAGATGGGACATGGCGTTGTGAGAAGTGTGACAAATGCGTGGATGAATGTGACTACAGGTATATATTACAGATCCAGTTACAGGACCATACAGACCTAACCTGGGCAACTGCATTCCAAGAAGCTGGTGAAGAGATAATGGGAATGTCTGCAAAAGATTTGTATTATGTGAAGTATGAAAATCAGGATGAGGAGAAATTTGAAGATATCATCCGCAGTGTCGCTTTTACTAAGTACATTTTCAAGCTGAAGATTAAAGAAGAAACTTACAGCGATGAGCAGCGGGTTAAAGCAACAGTTGTGAAAGCTGAAAAGCTGAATTATTCTTCAAATACCAGGTTCATGCTAGAGGCAATAGATAAACTCAAGATAGGAGATGCAAATTCTCTCCCTATAAAGGCAGAGAGTTCCAATTACAGGTCTGATGCATTTAATTCCGGTGTTGGAACCTCAGGAACTAGAGACACCGCATCAGTCGATGCACGCAGAGAATTTGGATTACCTGCAGCAAACCAAGTGGGTCAATATGGAAATCAGTACAGTAGTGATGCAAGGTCTCTTGGCGGTTTTACTAGCTGCAACGTGTGTCGCAGCAACAGCCATGTTTCTGCAAACTGCCCAACCCTTATGAGTGAGCCACAAGGACAATACATGGGTGGCACAAATGCAGGAGGAGGTATGCCAAGGCAACATGTAGGCAGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

853

Amino Acids

94.39

Weight (kDa)

7.76

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 109 3.2e-33 Replication factor-A protein 1, N-terminal domain
tRNA_anti-codon PF01336 312 - 399 3.3e-10 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 421 - 524 1.3e-27 Replication protein A OB domain
Rep_fac-A_C PF08646 582 - 732 1.9e-51 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 251
Acc36I ACCTGC 2 cut(s) 737, 2365
Acc65I GGTACC 1 cut(s) 1226
AccB1I GGYRCC 1 cut(s) 1226
AccB7I CCANNNNNTGG 1 cut(s) 389
AccII CGCG 1 cut(s) 991
AccIII TCCGGA 1 cut(s) 703
AclWI GGATC 3 cut(s) 1523, 1754, 1921
AcsI RAATTY 6 cut(s) 1129, 1523, 1786, 2051, 2236, 2345
AcuI CTGAAG 3 cut(s) 1367, 1608, 2123
AdeI CACNNNGTG 1 cut(s) 389
AfaI GTAC 5 cut(s) 688, 1228, 1368, 2090, 2393
AfiI CCNNNNNNNGG 9 cut(s) 389, 647, 648, 778, 926, 1936, 1957, 2251, 2271
AflIII ACRYGT 2 cut(s) 2436, 2545
AjnI CCWGG 4 cut(s) 361, 924, 1955, 2189
Alw21I GWGCWC 2 cut(s) 740, 1547
Alw26I GTCTC 4 cut(s) 56, 1383, 2310, 2415
AlwI GGATC 3 cut(s) 1523, 1754, 1921
AlwNI CAGNNNCTG 1 cut(s) 2559
Ama87I CYCGRG 1 cut(s) 777
Aor13HI TCCGGA 1 cut(s) 703
AoxI GGCC 1 cut(s) 1402
ApeKI GCWGC 9 cut(s) 182, 427, 908, 1833, 2134, 2360, 2430, 2445, 2553
ApoI RAATTY 6 cut(s) 1129, 1523, 1786, 2051, 2236, 2345
ArsI GACNNNNNNTTYG 2 cut(s) 1668, 1700
AseI ATTAAT 1 cut(s) 378
Asp700I GAANNNNTTC 1 cut(s) 1007
Asp718I GGTACC 1 cut(s) 1226
AspS9I GGNCC 5 cut(s) 769, 863, 936, 1725, 1939
AsuC2I CCSGG 1 cut(s) 1490
AsuHPI GGTGA 8 cut(s) 19, 540, 603, 705, 1063, 1277, 1532, 1994
AvaI CYCGRG 1 cut(s) 777
AvaII GGWCC 5 cut(s) 769, 863, 936, 1725, 1939
AxyI CCTNAGG 2 cut(s) 971, 2305
BanI GGYRCC 1 cut(s) 1226
BanII GRGCYC 2 cut(s) 740, 784
BauI CACGAG 1 cut(s) 955
Bbv12I GWGCWC 2 cut(s) 740, 1547
BbvCI CCTCAGC 2 cut(s) 552, 615
BbvI GCAGC 8 cut(s) 194, 439, 895, 1845, 2146, 2372, 2417, 2457
BccI CCATC 6 cut(s) 161, 947, 1028, 1217, 1331, 1853
BceAI ACGGC 1 cut(s) 1815
BciT130I CCWGG 4 cut(s) 363, 926, 1957, 2191
BclI TGATCA 1 cut(s) 1075
BcnI CCSGG 1 cut(s) 1490
BcoDI GTCTC 4 cut(s) 56, 1383, 2310, 2415
BfmI CTRYAG 2 cut(s) 1909, 2358
BfuAI ACCTGC 2 cut(s) 737, 2365
BisI GCNGC 9 cut(s) 183, 428, 909, 1834, 2135, 2361, 2431, 2446, 2554
BlsI GCNGC 9 cut(s) 184, 429, 910, 1835, 2136, 2362, 2432, 2447, 2555
Bme1390I CCNGG 5 cut(s) 363, 926, 1490, 1957, 2191
Bme18I GGWCC 5 cut(s) 769, 863, 936, 1725, 1939
BmeT110I CYCGRG 1 cut(s) 777
BmgT120I GGNCC 5 cut(s) 769, 863, 936, 1725, 1939
BmiI GGNNCC 5 cut(s) 783, 864, 1228, 1726, 2302
BmrFI CCNGG 5 cut(s) 363, 926, 1490, 1957, 2191
BmsI GCATC 9 cut(s) 63, 1018, 1057, 1205, 2221, 2269, 2323, 2333, 2392
Bpu10I CCTNAGC 2 cut(s) 552, 615
BpuEI CTTGAG 4 cut(s) 480, 543, 651, 2204
BpuMI CCSGG 1 cut(s) 1490
Bsa29I ATCGAT 1 cut(s) 145
BsaI GGTCTC 2 cut(s) 1383, 2415
BsaJI CCNNGG 6 cut(s) 362, 776, 890, 925, 1956, 2537
BsaWI WCCGGW 4 cut(s) 703, 811, 1664, 2291
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 9 cut(s) 389, 647, 648, 778, 926, 1936, 1957, 2251, 2271
Bse1I ACTGG 4 cut(s) 77, 694, 1748, 1930
Bse21I CCTNAGG 2 cut(s) 971, 2305
Bse3DI GCAATG 2 cut(s) 418, 1613
BseAI TCCGGA 1 cut(s) 703
BseBI CCWGG 4 cut(s) 363, 926, 1957, 2191
BseCI ATCGAT 1 cut(s) 145
BseDI CCNNGG 6 cut(s) 362, 776, 890, 925, 1956, 2537
BseGI GGATG 4 cut(s) 279, 1903, 2047, 2064
BseLI CCNNNNNNNGG 9 cut(s) 389, 647, 648, 778, 926, 1936, 1957, 2251, 2271
BseMI GCAATG 2 cut(s) 418, 1613
BseMII CTCAG 8 cut(s) 414, 566, 629, 962, 1157, 1353, 1743, 2319
BseNI ACTGG 4 cut(s) 77, 694, 1748, 1930
BseRI GAGGAG 3 cut(s) 876, 2060, 2541
BseXI GCAGC 8 cut(s) 194, 439, 895, 1845, 2146, 2372, 2417, 2457
BseYI CCCAGC 1 cut(s) 647
Bsh1236I CGCG 1 cut(s) 991
BshFI GGCC 1 cut(s) 1404
BshNI GGYRCC 1 cut(s) 1226
BshVI ATCGAT 1 cut(s) 145
BsiHKAI GWGCWC 2 cut(s) 740, 1547
BsiHKCI CYCGRG 1 cut(s) 777
BsiSI CCGG 6 cut(s) 704, 812, 1490, 1665, 1739, 2292
BslFI GGGAC 5 cut(s) 184, 624, 876, 1738, 1876
BslI CCNNNNNNNGG 9 cut(s) 389, 647, 648, 778, 926, 1936, 1957, 2251, 2271
BsmAI GTCTC 4 cut(s) 56, 1383, 2310, 2415
BsmFI GGGAC 5 cut(s) 184, 624, 876, 1738, 1876
BsmI GAATGC 3 cut(s) 195, 1636, 1967
BsnI GGCC 1 cut(s) 1404
Bso31I GGTCTC 2 cut(s) 1383, 2415
BsoBI CYCGRG 1 cut(s) 777
Bsp1286I GDGCHC 3 cut(s) 740, 784, 1547
Bsp13I TCCGGA 1 cut(s) 703
Bsp143I GATC 7 cut(s) 1018, 1075, 1515, 1704, 1746, 1822, 1926
BspANI GGCC 1 cut(s) 1404
BspCNI CTCAG 8 cut(s) 415, 565, 628, 963, 1156, 1354, 1742, 2318
BspDI ATCGAT 1 cut(s) 145
BspEI TCCGGA 1 cut(s) 703
BspFNI CGCG 1 cut(s) 991
BspHI TCATGA 2 cut(s) 1773, 1812
BspLI GGNNCC 5 cut(s) 783, 864, 1228, 1726, 2302
BspMAI CTGCAG 1 cut(s) 2362
BspMI ACCTGC 2 cut(s) 737, 2365
BspPI GGATC 3 cut(s) 1523, 1754, 1921
BspT107I GGYRCC 1 cut(s) 1226
BspTNI GGTCTC 2 cut(s) 1383, 2415
BsrDI GCAATG 2 cut(s) 418, 1613
BsrI ACTGG 4 cut(s) 77, 694, 1748, 1930
BssECI CCNNGG 6 cut(s) 362, 776, 890, 925, 1956, 2537
BssMI GATC 7 cut(s) 1018, 1075, 1515, 1704, 1746, 1822, 1926
BssSI CACGAG 1 cut(s) 955
BssT1I CCWWGG 1 cut(s) 2537
Bst2BI CACGAG 1 cut(s) 955
Bst2UI CCWGG 4 cut(s) 363, 926, 1957, 2191
Bst6I CTCTTC 2 cut(s) 1386, 1980
BstAPI GCANNNNNTGC 1 cut(s) 797
BstC8I GCNNGC 4 cut(s) 116, 1140, 1510, 2338
BstDEI CTNAG 9 cut(s) 423, 552, 615, 971, 1143, 1362, 1729, 2085, 2305
BstEII GGTNACC 1 cut(s) 1417
BstF5I GGATG 4 cut(s) 279, 1903, 2047, 2064
BstFNI CGCG 1 cut(s) 991
BstKTI GATC 7 cut(s) 1021, 1078, 1518, 1707, 1749, 1825, 1929
BstMAI GTCTC 4 cut(s) 56, 1383, 2310, 2415
BstMBI GATC 7 cut(s) 1018, 1075, 1515, 1704, 1746, 1822, 1926
BstMWI GCNNNNNNNGC 4 cut(s) 788, 797, 1505, 2451
BstNI CCWGG 4 cut(s) 363, 926, 1957, 2191
BstNSI RCATGY 1 cut(s) 2549
BstPI GGTNACC 1 cut(s) 1417
BstSCI CCNGG 5 cut(s) 361, 924, 1488, 1955, 2189
BstSFI CTRYAG 2 cut(s) 1909, 2358
BstUI CGCG 1 cut(s) 991
BstV1I GCAGC 8 cut(s) 194, 439, 895, 1845, 2146, 2372, 2417, 2457
BstX2I RGATCY 1 cut(s) 1926
BstXI CCANNNNNNTGG 1 cut(s) 1979
BstYI RGATCY 1 cut(s) 1926
Bsu15I ATCGAT 1 cut(s) 145
Bsu36I CCTNAGG 2 cut(s) 971, 2305
BsuRI GGCC 1 cut(s) 1404
BsuTUI ATCGAT 1 cut(s) 145
BtgZI GCGATG 1 cut(s) 2142
BtsCI GGATG 4 cut(s) 279, 1903, 2047, 2064
BtsI GCAGTG 1 cut(s) 2077
BtsIMutI CAGTG 3 cut(s) 84, 1296, 2077
BveI ACCTGC 2 cut(s) 737, 2365
Cac8I GCNNGC 4 cut(s) 116, 1140, 1510, 2338
CaiI CAGNNNCTG 1 cut(s) 2559
CciI TCATGA 2 cut(s) 1773, 1812
Cfr13I GGNCC 5 cut(s) 769, 863, 936, 1725, 1939
ClaI ATCGAT 1 cut(s) 145
CseI GACGC 4 cut(s) 527, 590, 608, 653
CsiI ACCWGGT 1 cut(s) 2189
Csp6I GTAC 5 cut(s) 687, 1227, 1367, 2089, 2392
CspCI CAANNNNNGTGG 2 cut(s) 2352, 2387
CviQI GTAC 5 cut(s) 687, 1227, 1367, 2089, 2392
DdeI CTNAG 9 cut(s) 423, 552, 615, 971, 1143, 1362, 1729, 2085, 2305
DpnI GATC 7 cut(s) 1020, 1077, 1517, 1706, 1748, 1824, 1928
DpnII GATC 7 cut(s) 1018, 1075, 1515, 1704, 1746, 1822, 1926
DraIII CACNNNGTG 1 cut(s) 389
Eam1104I CTCTTC 2 cut(s) 1386, 1980
EarI CTCTTC 2 cut(s) 1386, 1980
Ecl136II GAGCTC 1 cut(s) 738
Eco130I CCWWGG 1 cut(s) 2537
Eco24I GRGCYC 2 cut(s) 740, 784
Eco31I GGTCTC 2 cut(s) 1383, 2415
Eco32I GATATC 1 cut(s) 2062
Eco47I GGWCC 5 cut(s) 769, 863, 936, 1725, 1939
Eco53kI GAGCTC 1 cut(s) 738
Eco57I CTGAAG 3 cut(s) 1367, 1608, 2123
Eco81I CCTNAGG 2 cut(s) 971, 2305
Eco88I CYCGRG 1 cut(s) 777
Eco91I GGTNACC 1 cut(s) 1417
EcoICRI GAGCTC 1 cut(s) 738
EcoO109I RGGNCCY 2 cut(s) 863, 1725
EcoO65I GGTNACC 1 cut(s) 1417
EcoRI GAATTC 1 cut(s) 1523
EcoRII CCWGG 4 cut(s) 361, 924, 1955, 2189
EcoRV GATATC 1 cut(s) 2062
EcoT14I CCWWGG 1 cut(s) 2537
EcoT22I ATGCAT 1 cut(s) 2284
EcoT38I GRGCYC 2 cut(s) 740, 784
ErhI CCWWGG 1 cut(s) 2537
FaqI GGGAC 5 cut(s) 184, 624, 876, 1738, 1876
FauI CCCGC 2 cut(s) 1501, 2130
FbaI TGATCA 1 cut(s) 1075
Fnu4HI GCNGC 9 cut(s) 183, 428, 909, 1834, 2135, 2361, 2431, 2446, 2554
FokI GGATG 4 cut(s) 266, 1910, 2051, 2054
FriOI GRGCYC 2 cut(s) 740, 784
Fsp4HI GCNGC 9 cut(s) 183, 428, 909, 1834, 2135, 2361, 2431, 2446, 2554
GluI GCNGC 9 cut(s) 183, 428, 909, 1834, 2135, 2361, 2431, 2446, 2554
GsaI CCCAGC 1 cut(s) 651
HaeIII GGCC 1 cut(s) 1404
HapII CCGG 6 cut(s) 704, 812, 1490, 1665, 1739, 2292
HgaI GACGC 4 cut(s) 527, 590, 608, 653
HincII GTYRAC 2 cut(s) 400, 856
HindII GTYRAC 2 cut(s) 400, 856
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 7 cut(s) 106, 134, 220, 255, 286, 1190, 1475
HpaI GTTAAC 2 cut(s) 400, 856
HpaII CCGG 6 cut(s) 704, 812, 1490, 1665, 1739, 2292
HphI GGTGA 8 cut(s) 19, 540, 603, 705, 1063, 1277, 1532, 1994
Hpy166II GTNNAC 3 cut(s) 400, 856, 936
Hpy8I GTNNAC 3 cut(s) 400, 856, 936
Hpy99I CGWCG 3 cut(s) 521, 584, 647
HpyAV CCTTC 9 cut(s) 285, 435, 726, 992, 1384, 1442, 1616, 1620, 1771
HpyCH4IV ACGT 2 cut(s) 388, 2436
HpyF10VI GCNNNNNNNGC 4 cut(s) 788, 797, 1505, 2451
HpyF3I CTNAG 9 cut(s) 423, 552, 615, 971, 1143, 1362, 1729, 2085, 2305
HpySE526I ACGT 2 cut(s) 388, 2436
Kpn2I TCCGGA 1 cut(s) 703
KpnI GGTACC 1 cut(s) 1230
Ksp22I TGATCA 1 cut(s) 1075
KspAI GTTAAC 2 cut(s) 400, 856
Kzo9I GATC 7 cut(s) 1018, 1075, 1515, 1704, 1746, 1822, 1926
LmnI GCTCC 3 cut(s) 787, 889, 1553
Lsp1109I GCAGC 8 cut(s) 194, 439, 895, 1845, 2146, 2372, 2417, 2457
LweI GCATC 9 cut(s) 63, 1018, 1057, 1205, 2221, 2269, 2323, 2333, 2392
MabI ACCWGGT 1 cut(s) 2189
MaeII ACGT 2 cut(s) 388, 2436
MalI GATC 7 cut(s) 1020, 1077, 1517, 1706, 1748, 1824, 1928
MboI GATC 7 cut(s) 1018, 1075, 1515, 1704, 1746, 1822, 1926
MfeI CAATTG 3 cut(s) 236, 311, 1548
MflI RGATCY 1 cut(s) 1926
MhlI GDGCHC 3 cut(s) 740, 784, 1547
MlyI GAGTC 3 cut(s) 249, 280, 1184
MmeI TCCRAC 4 cut(s) 540, 603, 1355, 2278
Mph1103I ATGCAT 1 cut(s) 2284
MroI TCCGGA 1 cut(s) 703
MroXI GAANNNNTTC 1 cut(s) 1007
MspA1I CMGCKG 1 cut(s) 2137
MspI CCGG 6 cut(s) 704, 812, 1490, 1665, 1739, 2292
MspR9I CCNGG 5 cut(s) 363, 926, 1490, 1957, 2191
MunI CAATTG 3 cut(s) 236, 311, 1548
Mva1269I GAATGC 3 cut(s) 195, 1636, 1967
MvaI CCWGG 4 cut(s) 363, 926, 1957, 2191
MvnI CGCG 1 cut(s) 991
MwoI GCNNNNNNNGC 4 cut(s) 788, 797, 1505, 2451
NciI CCSGG 1 cut(s) 1490
NdeII GATC 7 cut(s) 1018, 1075, 1515, 1704, 1746, 1822, 1926
NlaIV GGNNCC 5 cut(s) 783, 864, 1228, 1726, 2302
NmuCI GTSAC 6 cut(s) 52, 952, 1051, 1843, 1883, 1904
NsiI ATGCAT 1 cut(s) 2284
NspI RCATGY 1 cut(s) 2549
PagI TCATGA 2 cut(s) 1773, 1812
PciI ACATGT 1 cut(s) 2545
PctI GAATGC 3 cut(s) 195, 1636, 1967
PdmI GAANNNNTTC 1 cut(s) 1007
PfeI GAWTC 4 cut(s) 106, 134, 220, 1475
PflMI CCANNNNNTGG 1 cut(s) 389
PkrI GCNGC 9 cut(s) 184, 429, 910, 1835, 2136, 2362, 2432, 2447, 2555
PleI GAGTC 3 cut(s) 249, 280, 1184
PpsI GAGTC 3 cut(s) 249, 280, 1184
PpuMI RGGWCCY 2 cut(s) 863, 1725
PscI ACATGT 1 cut(s) 2545
PshBI ATTAAT 1 cut(s) 378
PsiI TTATAA 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 740
Psp5II RGGWCCY 2 cut(s) 863, 1725
Psp6I CCWGG 4 cut(s) 361, 924, 1955, 2189
PspEI GGTNACC 1 cut(s) 1417
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 4 cut(s) 361, 924, 1955, 2189
PspN4I GGNNCC 5 cut(s) 783, 864, 1228, 1726, 2302
PspPI GGNCC 5 cut(s) 769, 863, 936, 1725, 1939
PspPPI RGGWCCY 2 cut(s) 863, 1725
PsrI GAACNNNNNNTAC 2 cut(s) 1476, 1508
PstI CTGCAG 1 cut(s) 2362
PstNI CAGNNNCTG 1 cut(s) 2559
PsuI RGATCY 1 cut(s) 1926
RsaI GTAC 5 cut(s) 688, 1228, 1368, 2090, 2393
RsaNI GTAC 5 cut(s) 687, 1227, 1367, 2089, 2392
SacI GAGCTC 1 cut(s) 740
SatI GCNGC 9 cut(s) 183, 428, 909, 1834, 2135, 2361, 2431, 2446, 2554
Sau3AI GATC 7 cut(s) 1018, 1075, 1515, 1704, 1746, 1822, 1926
Sau96I GGNCC 5 cut(s) 769, 863, 936, 1725, 1939
SchI GAGTC 3 cut(s) 249, 280, 1184
ScrFI CCNGG 5 cut(s) 363, 926, 1490, 1957, 2191
SduI GDGCHC 3 cut(s) 740, 784, 1547
SexAI ACCWGGT 1 cut(s) 2189
SfaNI GCATC 9 cut(s) 63, 1018, 1057, 1205, 2221, 2269, 2323, 2333, 2392
SfcI CTRYAG 2 cut(s) 1909, 2358
SinI GGWCC 5 cut(s) 769, 863, 936, 1725, 1939
SmlI CTYRAG 4 cut(s) 495, 558, 666, 2219
SmoI CTYRAG 4 cut(s) 495, 558, 666, 2219
SstI GAGCTC 1 cut(s) 740
StyD4I CCNGG 5 cut(s) 361, 924, 1488, 1955, 2189
StyI CCWWGG 1 cut(s) 2537
TaiI ACGT 2 cut(s) 391, 2439
TaqI TCGA 4 cut(s) 145, 289, 374, 2331
TatI WGTACW 2 cut(s) 2088, 2391
TfiI GAWTC 4 cut(s) 106, 134, 220, 1475
TscAI CASTG 3 cut(s) 84, 1303, 2077
TseFI GTSAC 6 cut(s) 52, 952, 1051, 1843, 1883, 1904
TseI GCWGC 9 cut(s) 182, 427, 908, 1833, 2134, 2360, 2430, 2445, 2553
Tsp45I GTSAC 6 cut(s) 52, 952, 1051, 1843, 1883, 1904
TspGWI ACGGA 4 cut(s) 32, 60, 107, 1869
TspRI CASTG 3 cut(s) 84, 1303, 2077
Van91I CCANNNNNTGG 1 cut(s) 389
VpaK11BI GGWCC 5 cut(s) 769, 863, 936, 1725, 1939
VspI ATTAAT 1 cut(s) 378
XapI RAATTY 6 cut(s) 1129, 1523, 1786, 2051, 2236, 2345
XbaI TCTAGA 1 cut(s) 1652
XceI RCATGY 1 cut(s) 2549
XmnI GAANNNNTTC 1 cut(s) 1007
Zsp2I ATGCAT 1 cut(s) 2284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.