Rh2BG015800

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009053
Physical Location & Seq
Forward (+)
1160795 .. 1162041
1247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG015800.1

Sequence Viewer

Length: 783 bp
ATGGACGTGAGGCTAACGGAGGGGGCGATACTGAAGATCACGACGGAAGACCATGACGAGCAGTATAGGCCCATCCTGCAAGTGCTCGACGTGAAGCAGATCCTCGCACAGAGCGGCGGCGGCGGCGCGGACAAAGAGCGCTACAGGCTGGCCTTGTCCGATGGGTCCCACACCCAGCAGGGGATGATTTCCACTCAGCAGAACTTCCTGATTCAGCAGGGCCACCTCCAGAAGGGCTCCGTCGTCTGCCTCAACCAATACACCTGCACGCCCGTCCGAGGCCGCCAGATCATCATTATAATCTCATTGGATGTGCTAGTTAATACATGTGAAATCATTGGACAGCCTGCCACATTTTCGAATGCACAGTCCCCAGCTACAATACCTGGAAATGCCCAATCTCTTAACAGCAGTGCTCGTGTGGCGGGTCATGAAACTGTGACTGAGGCAACTCCCCAACAACCTAGATTGAATCAATCGTATGGTGGTTCTTATTCTGGTGGTGTTGACCCAGGAAGATATGCTACAATGAGTACAGTTCCCAATCACCCTAGACCAGAGACTGGTTCTGGTTTTGCTGGGTTAGCACCAGTAAGTGGGTCATATGCTGGTCAGAACACAGGTTTACGCAGCCCCATATCCCAGGTTCCACCTCCACTTCAAAATTCTTATGCTCACCCACCTCAGCCGACTTATCAACAGTCACACTCAAATTCTTATGCCCGCCCACCTCAGCCGACTTATCAACAGTCACAATTAATTCAATCAAAACTTGGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

260

Amino Acids

27.9

Weight (kDa)

7.77

Isoelectric Point (pI)

59.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 107 2.6e-25 Replication factor-A protein 1, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 299
AarI CACCTGC 1 cut(s) 272
Acc36I ACCTGC 1 cut(s) 272
AccB7I CCANNNNNTGG 2 cut(s) 563, 596
AccBSI CCGCTC 1 cut(s) 114
AccII CGCG 1 cut(s) 128
AciI CCGC 8 cut(s) 114, 117, 120, 123, 128, 283, 425, 724
AclWI GGATC 1 cut(s) 94
AcsI RAATTY 2 cut(s) 664, 712
AcuI CTGAAG 1 cut(s) 53
AfaI GTAC 1 cut(s) 535
AfeI AGCGCT 1 cut(s) 140
AfiI CCNNNNNNNGG 5 cut(s) 180, 232, 278, 563, 596
AflIII ACRYGT 1 cut(s) 326
AgsI TTSAA 3 cut(s) 472, 662, 764
AjiI CACGTC 2 cut(s) 7, 91
AjnI CCWGG 3 cut(s) 385, 511, 642
AluBI AGCT 1 cut(s) 377
AluI AGCT 1 cut(s) 377
Alw21I GWGCWC 2 cut(s) 87, 418
Alw26I GTCTC 1 cut(s) 554
AlwI GGATC 1 cut(s) 94
AlwNI CAGNNNCTG 1 cut(s) 563
Aor51HI AGCGCT 1 cut(s) 140
AoxI GGCC 4 cut(s) 68, 150, 220, 280
ApeKI GCWGC 1 cut(s) 630
ApoI RAATTY 2 cut(s) 664, 712
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
AseI ATTAAT 1 cut(s) 758
AspLEI GCGC 2 cut(s) 128, 141
AspS9I GGNCC 3 cut(s) 69, 165, 220
AsuHPI GGTGA 2 cut(s) 539, 668
AsuII TTCGAA 1 cut(s) 359
AvaII GGWCC 1 cut(s) 165
BanII GRGCYC 1 cut(s) 239
BarI GAAGNNNNNNTAC 2 cut(s) 508, 540
BauI CACGAG 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 2 cut(s) 87, 418
BbvCI CCTCAGC 2 cut(s) 684, 732
BbvI GCAGC 1 cut(s) 642
BccI CCATC 2 cut(s) 80, 155
BciT130I CCWGG 3 cut(s) 387, 513, 644
BcoDI GTCTC 1 cut(s) 554
BfaI CTAG 3 cut(s) 317, 465, 552
BfmI CTRYAG 1 cut(s) 142
BfoI RGCGCY 1 cut(s) 142
BfuAI ACCTGC 1 cut(s) 272
BisI GCNGC 6 cut(s) 115, 118, 121, 124, 283, 631
BlsI GCNGC 6 cut(s) 116, 119, 122, 125, 284, 632
Bme1390I CCNGG 3 cut(s) 387, 513, 644
Bme18I GGWCC 1 cut(s) 165
BmgBI CACGTC 2 cut(s) 7, 91
BmgT120I GGNCC 3 cut(s) 69, 165, 220
BmiI GGNNCC 4 cut(s) 166, 167, 238, 648
BmrFI CCNGG 3 cut(s) 387, 513, 644
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 212
Bpu10I CCTNAGC 2 cut(s) 684, 732
Bpu14I TTCGAA 1 cut(s) 359
BsaJI CCNNGG 3 cut(s) 277, 511, 642
Bsc4I CCNNNNNNNGG 5 cut(s) 180, 232, 278, 563, 596
Bse1I ACTGG 2 cut(s) 568, 590
BseBI CCWGG 3 cut(s) 387, 513, 644
BseDI CCNNGG 3 cut(s) 277, 511, 642
BseGI GGATG 3 cut(s) 72, 189, 316
BseLI CCNNNNNNNGG 5 cut(s) 180, 232, 278, 563, 596
BseMII CTCAG 4 cut(s) 209, 435, 698, 746
BseNI ACTGG 2 cut(s) 568, 590
BseXI GCAGC 1 cut(s) 642
BseYI CCCAGC 3 cut(s) 174, 373, 578
BsgI GTGCAG 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 128
BshFI GGCC 4 cut(s) 70, 152, 222, 282
BsiHKAI GWGCWC 2 cut(s) 87, 418
BslFI GGGAC 2 cut(s) 151, 355
BslI CCNNNNNNNGG 5 cut(s) 180, 232, 278, 563, 596
BsmAI GTCTC 1 cut(s) 554
BsmFI GGGAC 2 cut(s) 151, 355
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 4 cut(s) 70, 152, 222, 282
Bsp119I TTCGAA 1 cut(s) 359
Bsp1286I GDGCHC 3 cut(s) 87, 239, 418
Bsp143I GATC 3 cut(s) 36, 99, 288
BspACI CCGC 8 cut(s) 114, 117, 120, 123, 128, 283, 425, 724
BspANI GGCC 4 cut(s) 70, 152, 222, 282
BspCNI CTCAG 4 cut(s) 208, 436, 697, 745
BspFNI CGCG 1 cut(s) 128
BspHI TCATGA 1 cut(s) 430
BspLI GGNNCC 4 cut(s) 166, 167, 238, 648
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 1 cut(s) 94
BspT104I TTCGAA 1 cut(s) 359
BsrBI CCGCTC 1 cut(s) 114
BsrI ACTGG 2 cut(s) 568, 590
BssECI CCNNGG 3 cut(s) 277, 511, 642
BssMI GATC 3 cut(s) 36, 99, 288
BssSI CACGAG 1 cut(s) 417
Bst2BI CACGAG 1 cut(s) 417
Bst2UI CCWGG 3 cut(s) 387, 513, 644
Bst4CI ACNGT 5 cut(s) 369, 439, 538, 702, 750
BstBI TTCGAA 1 cut(s) 359
BstC8I GCNNGC 4 cut(s) 150, 269, 348, 724
BstDEI CTNAG 4 cut(s) 195, 444, 684, 732
BstENI CCTNNNNNAGG 1 cut(s) 230
BstF5I GGATG 3 cut(s) 72, 189, 316
BstFNI CGCG 1 cut(s) 128
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 2 cut(s) 128, 141
BstKTI GATC 3 cut(s) 39, 102, 291
BstMAI GTCTC 1 cut(s) 554
BstMBI GATC 3 cut(s) 36, 99, 288
BstMWI GCNNNNNNNGC 7 cut(s) 67, 76, 120, 123, 145, 422, 584
BstNI CCWGG 3 cut(s) 387, 513, 644
BstNSI RCATGY 1 cut(s) 330
BstSCI CCNGG 3 cut(s) 385, 511, 642
BstSFI CTRYAG 1 cut(s) 142
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 1 cut(s) 642
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 1 cut(s) 99
BstYI RGATCY 1 cut(s) 99
BsuRI GGCC 4 cut(s) 70, 152, 222, 282
BtrI CACGTC 2 cut(s) 7, 91
BtsCI GGATG 3 cut(s) 72, 189, 316
BtsI GCAGTG 1 cut(s) 418
BtsIMutI CAGTG 1 cut(s) 418
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 4 cut(s) 150, 269, 348, 724
CaiI CAGNNNCTG 1 cut(s) 563
CciI TCATGA 1 cut(s) 430
CfoI GCGC 2 cut(s) 128, 141
Cfr13I GGNCC 3 cut(s) 69, 165, 220
Csp6I GTAC 1 cut(s) 534
CviAII CATG 3 cut(s) 53, 327, 431
CviQI GTAC 1 cut(s) 534
DdeI CTNAG 4 cut(s) 195, 444, 684, 732
DpnI GATC 3 cut(s) 38, 101, 290
DpnII GATC 3 cut(s) 36, 99, 288
Eco24I GRGCYC 1 cut(s) 239
Eco47I GGWCC 1 cut(s) 165
Eco47III AGCGCT 1 cut(s) 140
Eco57I CTGAAG 1 cut(s) 53
EcoNI CCTNNNNNAGG 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 165
EcoRII CCWGG 3 cut(s) 385, 511, 642
EcoT38I GRGCYC 1 cut(s) 239
FaeI CATG 3 cut(s) 56, 330, 434
FaqI GGGAC 2 cut(s) 151, 355
FatI CATG 3 cut(s) 52, 326, 430
FauI CCCGC 2 cut(s) 418, 731
FauNDI CATATG 1 cut(s) 604
Fnu4HI GCNGC 6 cut(s) 115, 118, 121, 124, 283, 631
FokI GGATG 3 cut(s) 59, 196, 323
FriOI GRGCYC 1 cut(s) 239
Fsp4HI GCNGC 6 cut(s) 115, 118, 121, 124, 283, 631
FspBI CTAG 3 cut(s) 317, 465, 552
GlaI GCGC 2 cut(s) 127, 140
GluI GCNGC 6 cut(s) 115, 118, 121, 124, 283, 631
GsaI CCCAGC 3 cut(s) 178, 377, 582
GsuI CTGGAG 1 cut(s) 212
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 4 cut(s) 70, 152, 222, 282
HhaI GCGC 2 cut(s) 128, 141
Hin1II CATG 3 cut(s) 56, 330, 434
Hin6I GCGC 2 cut(s) 126, 139
HinP1I GCGC 2 cut(s) 126, 139
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HinfI GANTC 2 cut(s) 211, 472
HphI GGTGA 2 cut(s) 539, 668
Hpy166II GTNNAC 2 cut(s) 508, 626
Hpy188I TCNGA 3 cut(s) 160, 278, 615
Hpy188III TCNNGA 4 cut(s) 40, 208, 229, 431
Hpy8I GTNNAC 2 cut(s) 508, 626
Hpy99I CGWCG 3 cut(s) 46, 92, 245
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 5 cut(s) 369, 439, 538, 702, 750
HpyCH4IV ACGT 2 cut(s) 6, 90
HpyCH4V TGCA 3 cut(s) 79, 267, 365
HpyF10VI GCNNNNNNNGC 7 cut(s) 67, 76, 120, 123, 145, 422, 584
HpyF3I CTNAG 4 cut(s) 195, 444, 684, 732
HpySE526I ACGT 2 cut(s) 6, 90
Hsp92II CATG 3 cut(s) 56, 330, 434
HspAI GCGC 2 cut(s) 126, 139
KflI GGGWCCC 1 cut(s) 165
Kzo9I GATC 3 cut(s) 36, 99, 288
LmnI GCTCC 1 cut(s) 242
Lsp1109I GCAGC 1 cut(s) 642
MaeI CTAG 3 cut(s) 317, 465, 552
MaeII ACGT 2 cut(s) 6, 90
MaeIII GTNAC 3 cut(s) 439, 702, 750
MalI GATC 3 cut(s) 38, 101, 290
MbiI CCGCTC 1 cut(s) 114
MboI GATC 3 cut(s) 36, 99, 288
MboII GAAGA 3 cut(s) 46, 59, 528
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 3 cut(s) 87, 239, 418
MluCI AATT 4 cut(s) 664, 712, 755, 759
MseI TTAA 3 cut(s) 321, 405, 758
MspR9I CCNGG 3 cut(s) 387, 513, 644
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 3 cut(s) 387, 513, 644
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 7 cut(s) 67, 76, 120, 123, 145, 422, 584
NdeI CATATG 1 cut(s) 604
NdeII GATC 3 cut(s) 36, 99, 288
NlaIII CATG 3 cut(s) 56, 330, 434
NlaIV GGNNCC 4 cut(s) 166, 167, 238, 648
NmuCI GTSAC 3 cut(s) 439, 702, 750
NspI RCATGY 1 cut(s) 330
NspV TTCGAA 1 cut(s) 359
PagI TCATGA 1 cut(s) 430
PaqCI CACCTGC 1 cut(s) 272
PciI ACATGT 1 cut(s) 326
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 1 cut(s) 367
PfeI GAWTC 2 cut(s) 211, 472
PflMI CCANNNNNTGG 2 cut(s) 563, 596
PkrI GCNGC 6 cut(s) 116, 119, 122, 125, 284, 632
PpuMI RGGWCCY 1 cut(s) 165
PscI ACATGT 1 cut(s) 326
PshBI ATTAAT 1 cut(s) 758
PsiI TTATAA 1 cut(s) 299
Psp5II RGGWCCY 1 cut(s) 165
Psp6I CCWGG 3 cut(s) 385, 511, 642
PspFI CCCAGC 3 cut(s) 174, 373, 578
PspGI CCWGG 3 cut(s) 385, 511, 642
PspN4I GGNNCC 4 cut(s) 166, 167, 238, 648
PspPI GGNCC 3 cut(s) 69, 165, 220
PspPPI RGGWCCY 1 cut(s) 165
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 1 cut(s) 99
RsaI GTAC 1 cut(s) 535
RsaNI GTAC 1 cut(s) 534
SaqAI TTAA 3 cut(s) 321, 405, 758
SatI GCNGC 6 cut(s) 115, 118, 121, 124, 283, 631
Sau3AI GATC 3 cut(s) 36, 99, 288
Sau96I GGNCC 3 cut(s) 69, 165, 220
ScrFI CCNGG 3 cut(s) 387, 513, 644
SduI GDGCHC 3 cut(s) 87, 239, 418
SfcI CTRYAG 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 359
SinI GGWCC 1 cut(s) 165
Sse9I AATT 4 cut(s) 664, 712, 755, 759
SsiI CCGC 8 cut(s) 114, 117, 120, 123, 128, 283, 425, 724
SspMI CTAG 3 cut(s) 317, 465, 552
StyD4I CCNGG 3 cut(s) 385, 511, 642
TaaI ACNGT 5 cut(s) 369, 439, 538, 702, 750
TaiI ACGT 2 cut(s) 9, 93
TaqI TCGA 2 cut(s) 87, 359
TasI AATT 4 cut(s) 664, 712, 755, 759
TatI WGTACW 1 cut(s) 533
TauI GCSGC 5 cut(s) 117, 120, 123, 126, 285
TfiI GAWTC 2 cut(s) 211, 472
Tru1I TTAA 3 cut(s) 321, 405, 758
Tru9I TTAA 3 cut(s) 321, 405, 758
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 3 cut(s) 439, 702, 750
TseI GCWGC 1 cut(s) 630
Tsp45I GTSAC 3 cut(s) 439, 702, 750
TspDTI ATGAA 1 cut(s) 447
TspGWI ACGGA 3 cut(s) 32, 59, 229
TspRI CASTG 1 cut(s) 418
Van91I CCANNNNNTGG 2 cut(s) 563, 596
VpaK11BI GGWCC 1 cut(s) 165
VspI ATTAAT 1 cut(s) 758
XagI CCTNNNNNAGG 1 cut(s) 230
XapI RAATTY 2 cut(s) 664, 712
XceI RCATGY 1 cut(s) 330
XspI CTAG 3 cut(s) 317, 465, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.