MD06G1015200.v1.1

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009053
Physical Location & Seq
Reverse (-)
1938586 .. 1942209
3624 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1015200.v1.1.491

Sequence Viewer

Length: 2610 bp
ATGGATGTGAGGTTAACAGAAGGGGCGATAATGAAGATGTGCACAGAGGAGCTCAACGACGGGAGCTTTCAGCCCATCCTGCAAGTGATGGACGTGAAGCTGGTCCATACGGCGGCGCGAAATGGAGCTCAGCCGGGCCAAGATAAGGAGAGATACAGGGTTGTGATCTCCGACGGGTCGCACTACCAGCAGGGGATGCTGGGCACCCAGAAGAACACACTGGTTCGGCAAGGTATGCTTCAGAGCGGCTCCATCGTCTGCCTCAAACAGTTCACCTGCAACGCAATCCAAAACCGCCTGATTATCATTATAATTGAATTGGATTTGGTGCTTGACAAATGTGATTTCATTGGCAATCCTGTACCGGGACCTAAGTATTCGAATGCACAGTCGTCAGGGACGTCACCTGGAAATTCTCAATCTCTTGGTGGCACTGCAAATTCTGGCAGCACAGTTCATCAAACTGGCACGGGGGCTACTCCAGAACAGCCTAGAGTAAATCAATCATTTGGTAGTGCAGCTAGTGCTTACTCTAGTAATGCTGAGCCAGGAAGAAATGCTACTATGAATACACCTTCCAATTACCCTAAACCAGAGTCTGGATTTGGTTTTCCTGGATCAGCACCATCAAGTGGGTCATATGGCAATCAGAGCATAGGTTTTCACAACCCTAGATCTGAAGTTCCTCAGCCACCACCAAATTCATATGCCCGCCCACCTCAGCCCGCATATCAGCAGCCACCTCCGGTGTATACTAATAGAGGACCAGTTGCCAAAAATGATGCTCCTCCAAGAATAATGCCAATTGCTGCTCTGAATCCATATCAAGGAAGGTGGACAATCAAGGCTAGAGTGACAGCTAAAGGAGAACTTAGGCGCTATAACAATACTCGTGGCGATGGGAAAGTATTTTCTTTTGATCTTCTTGATGCTCATAATGGAGAAATTCGAGCAACCTGTTTTAATGCTGTGGCTGATCAATTCTACAACCAGATTGAAGTTGGTAAGATTTATTTGGTCTCCAGGGGTAGTTTAAAACCAGCTCAAAAGAATTTCAACCACCTCAACAATGACCATGAAATTCTTTTAGATAACTCATCAGTAATACAGTCATGTTTTGAGGATGATGGTTCGATTCCACATCATAATTTCAATTTCAGTCCAATAAATCTTGTTGAAGGCTTGGAGAACAACACTATTGTGGATGTAATAGGTGTGGTATCATTTATCAGTCCCCCTGCCTCTATAATGAGGAAAAATGGTGTTGAGACTCAGAAGAGAACCCTCCACTTGAAGGACATGTCTGGTCGTAGTGTTGAAGTAACTCTTTGGGGAAATTTGTGCAATGCAGAAGGACAAAGACTGCAGGTTATGTGTGATTCTGGTCTATTCCCGGTGCTTGCTGTAAAAGGTGCTAGAGTCAATGACTTCAATGGGAAGGCGGTGGGGACCATTTCCACAAGTCAGCTTCTGATAGAGCCTAATATCGAGAAGGCTCAGGAGGTGAGGAACTGGTTTGAGAAGGAAGGAAAGAATACCTCATGTATCTCTATTTCCAGGGAAACGGCAGGTGTGGGTAGAACAGAGAACCGAAAGACGTTATCCCAAATTAAAGATGAGAAGCTAGGGACTTCCGAGAAGCCAGATTGGATCACAGTTGGTGCAACCATTTCATTTATAAAGGTTGATAGTTTTTGCTATTCAGCATGCCCACTCATGATTGGTGATCGACAATGCAGCAAAAAGGTTACAAATAATGGAGATGGGACTTGGAGGTGTGAAAGATGCAATGAGTCCGTAGAAGCATGTGACTACAGATATATACTCCAGTTTCAGATACAGGACCATACTGACTCAACATGGGTAACTGCATTTCAGGAGGGTGGTGAGGAAATCATGGGAATACCTGCAAAAGATTTGTATTTTATCAAATTTGAAGAGCAAGATGATGAGAAGTTTGCAGAAATCATTCAGAAGGTTCTCTTTACCAAATTCATTTTCAAGTTGAAAGTAAAGGAGGAGACTTTTAGTGATGAGCAACGTGTAAAATCAACAGTTATCAAAGCCGAGAAAGTGAACCATTCATCGCATGCTAGATTTCATTTGGAATTGATGGATAAACTTAAGATGGAAAATATTAGTTCTTCTGCCTCCAAAGGGGACAATCTAATGCCTAGCGCCGGAATGAATGTTGCTGGAGTCGGGAACACTGCAATTAGACAGCCCACCCCTGCTGTGAATGTTGGTTATAGTAACAATGCTGGTAGAGAATTTGGAGCACCAGAAAATCAAGCGGGTCACCATGGGAATCAGTATGGCGGTACGTTTCTTTCAGGGGGTTCTGCTGGTTCATATCAAATATGTAAGAGTTGCGGGGATGCTGGCCATAGCTCAATGAACTGCCCAACTGTAATGAATGGCGCAGGACAGTCATCAGGAGTGGGCTATAACAATAGAGTGTCTTCTAGGCCAGGTATCAGCAATACTTCTGGTGAATGCTACAAATGCCACCAACCTGGACACTGGGCAAGAGACTGTCCTGGGTTAAACACTACTCCAGCTTATGGAAGCAGTGGAATCAACCGTGGGACATATGGTGTTTCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

870

Amino Acids

94.55

Weight (kDa)

7.75

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 110 2.4e-24 Replication factor-A protein 1, N-terminal domain
tRNA_anti-codon PF01336 280 - 351 4e-09 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 390 - 492 1.2e-26 Replication protein A OB domain
Rep_fac-A_C PF08646 550 - 698 1.1e-52 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 311, 1679
AarI CACCTGC 2 cut(s) 284, 1559
AatII GACGTC 1 cut(s) 404
Acc36I ACCTGC 4 cut(s) 284, 1357, 1559, 1915
AccB1I GGYRCC 1 cut(s) 203
AccB7I CCANNNNNTGG 3 cut(s) 599, 632, 2564
AccBSI CCGCTC 1 cut(s) 246
AccI GTMKAC 1 cut(s) 752
AccII CGCG 1 cut(s) 118
AciI CCGC 9 cut(s) 113, 246, 295, 712, 726, 1442, 2294, 2319, 2373
AclWI GGATC 2 cut(s) 625, 1658
AcoI YGGCCR 1 cut(s) 2383
AcuI CTGAAG 2 cut(s) 224, 699
AcyI GRCGYC 1 cut(s) 401
AfaI GTAC 2 cut(s) 363, 2323
AflII CTTAAG 1 cut(s) 2123
AflIII ACRYGT 2 cut(s) 1299, 2041
AjiI CACGTC 1 cut(s) 94
AjnI CCWGG 8 cut(s) 406, 547, 613, 1022, 1556, 2470, 2515, 2539
AjuI GAANNNNNNNTTGG 2 cut(s) 1982, 2014
AleI CACNNNNGTG 1 cut(s) 1199
Alw21I GWGCWC 4 cut(s) 44, 54, 130, 2281
Alw26I GTCTC 4 cut(s) 1024, 1262, 2015, 2526
Alw44I GTGCAC 1 cut(s) 40
AlwI GGATC 2 cut(s) 625, 1658
AlwNI CAGNNNCTG 2 cut(s) 599, 1471
AoxI GGCC 3 cut(s) 136, 2383, 2468
ApaLI GTGCAC 1 cut(s) 40
ApeKI GCWGC 5 cut(s) 447, 518, 736, 809, 1737
ArsI GACNNNNNNTTYG 2 cut(s) 374, 406
Asp700I GAANNNNTTC 1 cut(s) 1968
AspLEI GCGC 4 cut(s) 118, 879, 2180, 2423
AspS9I GGNCC 6 cut(s) 103, 136, 368, 764, 1449, 1843
AsuC2I CCSGG 3 cut(s) 135, 366, 1394
AsuHPI GGTGA 7 cut(s) 265, 396, 1516, 1736, 1898, 2291, 2504
AsuII TTCGAA 1 cut(s) 380
AvaII GGWCC 5 cut(s) 103, 368, 764, 1449, 1843
BaeGI GKGCMC 2 cut(s) 44, 206
BalI TGGCCA 1 cut(s) 2385
BanI GGYRCC 1 cut(s) 203
BanII GRGCYC 2 cut(s) 54, 130
BarI GAAGNNNNNNTAC 2 cut(s) 544, 576
BauI CACGAG 1 cut(s) 891
BbsI GAAGAC 1 cut(s) 2454
Bbv12I GWGCWC 4 cut(s) 44, 54, 130, 2281
BbvCI CCTCAGC 2 cut(s) 687, 720
BbvI GCAGC 5 cut(s) 459, 530, 748, 796, 1749
BccI CCATC 9 cut(s) 82, 83, 260, 634, 893, 1121, 1757, 2107, 2122
BceAI ACGGC 2 cut(s) 126, 1581
BciT130I CCWGG 8 cut(s) 408, 549, 615, 1024, 1558, 2472, 2517, 2541
BclI TGATCA 1 cut(s) 976
BcnI CCSGG 3 cut(s) 135, 366, 1394
BcoDI GTCTC 4 cut(s) 1024, 1262, 2015, 2526
BfmI CTRYAG 2 cut(s) 1364, 1813
BfoI RGCGCY 2 cut(s) 880, 2181
BfrI CTTAAG 1 cut(s) 2123
BfuAI ACCTGC 4 cut(s) 284, 1357, 1559, 1915
BglII AGATCT 1 cut(s) 674
BisI GCNGC 7 cut(s) 114, 247, 448, 519, 737, 810, 1738
BlpI GCTNAGC 2 cut(s) 129, 543
BlsI GCNGC 7 cut(s) 115, 248, 449, 520, 738, 811, 1739
Bme18I GGWCC 5 cut(s) 103, 368, 764, 1449, 1843
BmgBI CACGTC 1 cut(s) 94
BmgT120I GGNCC 6 cut(s) 103, 136, 368, 764, 1449, 1843
BmiI GGNNCC 4 cut(s) 205, 250, 369, 1450
BmrI ACTGGG 1 cut(s) 2533
BmsI GCATC 5 cut(s) 186, 772, 919, 1775, 2368
BmuI ACTGGG 1 cut(s) 2533
BpiI GAAGAC 1 cut(s) 2454
BpmI CTGGAG 5 cut(s) 465, 1006, 1811, 2217, 2541
Bpu10I CCTNAGC 3 cut(s) 687, 720, 1497
Bpu1102I GCTNAGC 2 cut(s) 129, 543
Bpu14I TTCGAA 1 cut(s) 380
BpuMI CCSGG 3 cut(s) 135, 366, 1394
BsaHI GRCGYC 1 cut(s) 401
BsaI GGTCTC 1 cut(s) 1024
BsaJI CCNNGG 5 cut(s) 1023, 1557, 2302, 2540, 2584
BsaWI WCCGGW 1 cut(s) 745
Bse1I ACTGG 6 cut(s) 225, 469, 767, 1517, 1828, 2528
Bse3DI GCAATG 2 cut(s) 1351, 1795
BseBI CCWGG 8 cut(s) 408, 549, 615, 1024, 1558, 2472, 2517, 2541
BseDI CCNNGG 5 cut(s) 1023, 1557, 2302, 2540, 2584
BseGI GGATG 6 cut(s) 10, 75, 201, 1129, 1210, 2383
BseMI GCAATG 2 cut(s) 1351, 1795
BseMII CTCAG 6 cut(s) 143, 534, 701, 734, 1286, 1511
BseNI ACTGG 6 cut(s) 225, 469, 767, 1517, 1828, 2528
BseRI GAGGAG 3 cut(s) 62, 777, 2033
BseSI GKGCMC 2 cut(s) 44, 206
BseXI GCAGC 5 cut(s) 459, 530, 748, 796, 1749
BseYI CCCAGC 1 cut(s) 199
BsgI GTGCAG 1 cut(s) 537
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 3 cut(s) 138, 2385, 2470
BshNI GGYRCC 1 cut(s) 203
BsiHKAI GWGCWC 4 cut(s) 44, 54, 130, 2281
BsiSI CCGG 5 cut(s) 134, 365, 746, 1394, 2181
BslFI GGGAC 8 cut(s) 381, 412, 1218, 1462, 1642, 1780, 2174, 2602
BsmAI GTCTC 4 cut(s) 1024, 1262, 2015, 2526
BsmFI GGGAC 8 cut(s) 381, 412, 1218, 1462, 1642, 1780, 2174, 2602
BsmI GAATGC 2 cut(s) 388, 2501
BsnI GGCC 3 cut(s) 138, 2385, 2470
Bso31I GGTCTC 1 cut(s) 1024
Bsp119I TTCGAA 1 cut(s) 380
Bsp1286I GDGCHC 5 cut(s) 44, 54, 130, 206, 2281
Bsp143I GATC 7 cut(s) 165, 617, 674, 919, 976, 1650, 1726
Bsp1720I GCTNAGC 2 cut(s) 129, 543
Bsp19I CCATGG 1 cut(s) 2302
BspACI CCGC 9 cut(s) 113, 246, 295, 712, 726, 1442, 2294, 2319, 2373
BspANI GGCC 3 cut(s) 138, 2385, 2470
BspCNI CTCAG 6 cut(s) 142, 535, 700, 733, 1285, 1510
BspFNI CGCG 1 cut(s) 118
BspHI TCATGA 1 cut(s) 1716
BspLI GGNNCC 4 cut(s) 205, 250, 369, 1450
BspMAI CTGCAG 1 cut(s) 1368
BspMI ACCTGC 4 cut(s) 284, 1357, 1559, 1915
BspPI GGATC 2 cut(s) 625, 1658
BspQI GCTCTTC 1 cut(s) 1932
BspT104I TTCGAA 1 cut(s) 380
BspT107I GGYRCC 1 cut(s) 203
BspTI CTTAAG 1 cut(s) 2123
BspTNI GGTCTC 1 cut(s) 1024
BsrBI CCGCTC 1 cut(s) 246
BsrDI GCAATG 2 cut(s) 1351, 1795
BsrI ACTGG 6 cut(s) 225, 469, 767, 1517, 1828, 2528
BssECI CCNNGG 5 cut(s) 1023, 1557, 2302, 2540, 2584
BssMI GATC 7 cut(s) 165, 617, 674, 919, 976, 1650, 1726
BssNAI GTATAC 1 cut(s) 753
BssNI GRCGYC 1 cut(s) 401
BssSI CACGAG 1 cut(s) 891
BssT1I CCWWGG 1 cut(s) 2302
Bst1107I GTATAC 1 cut(s) 753
Bst2BI CACGAG 1 cut(s) 891
Bst2UI CCWGG 8 cut(s) 408, 549, 615, 1024, 1558, 2472, 2517, 2541
Bst6I CTCTTC 2 cut(s) 1271, 1932
BstACI GRCGYC 1 cut(s) 401
BstAFI CTTAAG 1 cut(s) 2123
BstAPI GCANNNNNTGC 3 cut(s) 196, 235, 524
BstBI TTCGAA 1 cut(s) 380
BstC8I GCNNGC 6 cut(s) 712, 726, 1401, 1708, 2091, 2383
BstDEI CTNAG 8 cut(s) 129, 372, 543, 687, 720, 872, 1272, 1497
BstDSI CCRYGG 2 cut(s) 2302, 2584
BstEII GGTNACC 1 cut(s) 2297
BstF5I GGATG 6 cut(s) 10, 75, 201, 1129, 1210, 2383
BstFNI CGCG 1 cut(s) 118
BstH2I RGCGCY 2 cut(s) 880, 2181
BstHHI GCGC 4 cut(s) 118, 879, 2180, 2423
BstKTI GATC 7 cut(s) 168, 620, 677, 922, 979, 1653, 1729
BstMAI GTCTC 4 cut(s) 1024, 1262, 2015, 2526
BstMBI GATC 7 cut(s) 165, 617, 674, 919, 976, 1650, 1726
BstMWI GCNNNNNNNGC 7 cut(s) 79, 187, 196, 235, 524, 651, 2505
BstNI CCWGG 8 cut(s) 408, 549, 615, 1024, 1558, 2472, 2517, 2541
BstNSI RCATGY 4 cut(s) 1303, 1710, 1809, 2093
BstPI GGTNACC 1 cut(s) 2297
BstSFI CTRYAG 2 cut(s) 1364, 1813
BstSLI GKGCMC 2 cut(s) 44, 206
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 5 cut(s) 459, 530, 748, 796, 1749
BstV2I GAAGAC 1 cut(s) 2454
BstX2I RGATCY 1 cut(s) 674
BstXI CCANNNNNNTGG 1 cut(s) 2516
BstYI RGATCY 1 cut(s) 674
BstZ17I GTATAC 1 cut(s) 753
BsuRI GGCC 3 cut(s) 138, 2385, 2470
BtgI CCRYGG 2 cut(s) 2302, 2584
BtgZI GCGATG 2 cut(s) 912, 2070
BtrI CACGTC 1 cut(s) 94
BtsCI GGATG 6 cut(s) 10, 75, 201, 1129, 1210, 2383
BtsI GCAGTG 3 cut(s) 432, 2208, 2578
BtsIMutI CAGTG 5 cut(s) 218, 432, 2208, 2521, 2578
BveI ACCTGC 4 cut(s) 284, 1357, 1559, 1915
Cac8I GCNNGC 6 cut(s) 712, 726, 1401, 1708, 2091, 2383
CaiI CAGNNNCTG 2 cut(s) 599, 1471
CciI TCATGA 1 cut(s) 1716
CfoI GCGC 4 cut(s) 118, 879, 2180, 2423
Cfr13I GGNCC 6 cut(s) 103, 136, 368, 764, 1449, 1843
Csp6I GTAC 2 cut(s) 362, 2322
CspCI CAANNNNNGTGG 4 cut(s) 815, 850, 874, 909
CviQI GTAC 2 cut(s) 362, 2322
DdeI CTNAG 8 cut(s) 129, 372, 543, 687, 720, 872, 1272, 1497
DpnI GATC 7 cut(s) 167, 619, 676, 921, 978, 1652, 1728
DpnII GATC 7 cut(s) 165, 617, 674, 919, 976, 1650, 1726
DraI TTTAAA 1 cut(s) 1035
EaeI YGGCCR 1 cut(s) 2383
Eam1104I CTCTTC 2 cut(s) 1271, 1932
EarI CTCTTC 2 cut(s) 1271, 1932
Ecl136II GAGCTC 2 cut(s) 52, 128
Eco130I CCWWGG 1 cut(s) 2302
Eco24I GRGCYC 2 cut(s) 54, 130
Eco31I GGTCTC 1 cut(s) 1024
Eco47I GGWCC 5 cut(s) 103, 368, 764, 1449, 1843
Eco53kI GAGCTC 2 cut(s) 52, 128
Eco57I CTGAAG 2 cut(s) 224, 699
Eco91I GGTNACC 1 cut(s) 2297
EcoICRI GAGCTC 2 cut(s) 52, 128
EcoO109I RGGNCCY 1 cut(s) 368
EcoO65I GGTNACC 1 cut(s) 2297
EcoRII CCWGG 8 cut(s) 406, 547, 613, 1022, 1556, 2470, 2515, 2539
EcoT14I CCWWGG 1 cut(s) 2302
EcoT38I GRGCYC 2 cut(s) 54, 130
ErhI CCWWGG 1 cut(s) 2302
FalI AAGNNNNNCTT 8 cut(s) 222, 254, 855, 887, 1311, 1343, 1967, 1999
FaqI GGGAC 8 cut(s) 381, 412, 1218, 1462, 1642, 1780, 2174, 2602
FauI CCCGC 4 cut(s) 719, 733, 2287, 2366
FauNDI CATATG 3 cut(s) 640, 706, 2593
FbaI TGATCA 1 cut(s) 976
FblI GTMKAC 1 cut(s) 752
Fnu4HI GCNGC 7 cut(s) 114, 247, 448, 519, 737, 810, 1738
FokI GGATG 6 cut(s) 17, 62, 208, 1136, 1217, 2390
FriOI GRGCYC 2 cut(s) 54, 130
Fsp4HI GCNGC 7 cut(s) 114, 247, 448, 519, 737, 810, 1738
GlaI GCGC 4 cut(s) 117, 878, 2179, 2422
GluI GCNGC 7 cut(s) 114, 247, 448, 519, 737, 810, 1738
GsaI CCCAGC 1 cut(s) 203
GsuI CTGGAG 5 cut(s) 465, 1006, 1811, 2217, 2541
HaeII RGCGCY 2 cut(s) 880, 2181
HaeIII GGCC 3 cut(s) 138, 2385, 2470
HapII CCGG 5 cut(s) 134, 365, 746, 1394, 2181
HhaI GCGC 4 cut(s) 118, 879, 2180, 2423
Hin1I GRCGYC 1 cut(s) 401
Hin6I GCGC 4 cut(s) 116, 877, 2178, 2421
HinP1I GCGC 4 cut(s) 116, 877, 2178, 2421
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HpaI GTTAAC 1 cut(s) 15
HpaII CCGG 5 cut(s) 134, 365, 746, 1394, 2181
HphI GGTGA 7 cut(s) 265, 396, 1516, 1736, 1898, 2291, 2504
Hpy166II GTNNAC 6 cut(s) 15, 42, 273, 753, 837, 2077
Hpy188III TCNNGA 9 cut(s) 482, 600, 926, 1489, 1499, 1717, 1877, 2203, 2436
Hpy8I GTNNAC 6 cut(s) 15, 42, 273, 753, 837, 2077
Hpy99I CGWCG 2 cut(s) 62, 176
HpyCH4IV ACGT 5 cut(s) 93, 401, 1598, 2041, 2324
HpyF10VI GCNNNNNNNGC 7 cut(s) 79, 187, 196, 235, 524, 651, 2505
HpyF3I CTNAG 8 cut(s) 129, 372, 543, 687, 720, 872, 1272, 1497
HpySE526I ACGT 5 cut(s) 93, 401, 1598, 2041, 2324
Hsp92I GRCGYC 1 cut(s) 401
HspAI GCGC 4 cut(s) 116, 877, 2178, 2421
Ksp22I TGATCA 1 cut(s) 976
KspAI GTTAAC 1 cut(s) 15
Kzo9I GATC 7 cut(s) 165, 617, 674, 919, 976, 1650, 1726
LguI GCTCTTC 1 cut(s) 1932
LmnI GCTCC 6 cut(s) 49, 63, 125, 254, 790, 2276
Lsp1109I GCAGC 5 cut(s) 459, 530, 748, 796, 1749
LweI GCATC 5 cut(s) 186, 772, 919, 1775, 2368
MaeII ACGT 5 cut(s) 93, 401, 1598, 2041, 2324
MaeIII GTNAC 8 cut(s) 402, 853, 1321, 1747, 1808, 1864, 2252, 2297
MalI GATC 7 cut(s) 167, 619, 676, 921, 978, 1652, 1728
MbiI CCGCTC 1 cut(s) 246
MboI GATC 7 cut(s) 165, 617, 674, 919, 976, 1650, 1726
MboII GAAGA 8 cut(s) 46, 223, 564, 914, 1288, 1949, 2136, 2454
MfeI CAATTG 1 cut(s) 804
MflI RGATCY 1 cut(s) 674
MhlI GDGCHC 5 cut(s) 44, 54, 130, 206, 2281
MlsI TGGCCA 1 cut(s) 2385
MluNI TGGCCA 1 cut(s) 2385
MlyI GAGTC 6 cut(s) 605, 1264, 1428, 1802, 1847, 2208
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 1 cut(s) 2385
MroXI GAANNNNTTC 1 cut(s) 1968
MscI TGGCCA 1 cut(s) 2385
MseI TTAA 7 cut(s) 14, 963, 1034, 1611, 2124, 2546, 2608
MslI CAYNNNNRTG 1 cut(s) 1199
Msp20I TGGCCA 1 cut(s) 2385
MspCI CTTAAG 1 cut(s) 2123
MspI CCGG 5 cut(s) 134, 365, 746, 1394, 2181
MunI CAATTG 1 cut(s) 804
Mva1269I GAATGC 2 cut(s) 388, 2501
MvaI CCWGG 8 cut(s) 408, 549, 615, 1024, 1558, 2472, 2517, 2541
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 7 cut(s) 79, 187, 196, 235, 524, 651, 2505
NciI CCSGG 3 cut(s) 135, 366, 1394
NcoI CCATGG 1 cut(s) 2302
NdeI CATATG 3 cut(s) 640, 706, 2593
NdeII GATC 7 cut(s) 165, 617, 674, 919, 976, 1650, 1726
NlaIV GGNNCC 4 cut(s) 205, 250, 369, 1450
NmeAIII GCCGAG 1 cut(s) 2092
NmuCI GTSAC 4 cut(s) 402, 853, 1808, 2297
NspI RCATGY 4 cut(s) 1303, 1710, 1809, 2093
NspV TTCGAA 1 cut(s) 380
OliI CACNNNNGTG 1 cut(s) 1199
PaeI GCATGC 2 cut(s) 1710, 2093
PagI TCATGA 1 cut(s) 1716
PaqCI CACCTGC 2 cut(s) 284, 1559
PciI ACATGT 1 cut(s) 1299
PciSI GCTCTTC 1 cut(s) 1932
PcsI WCGNNNNNNNCGW 1 cut(s) 398
PctI GAATGC 2 cut(s) 388, 2501
PdmI GAANNNNTTC 1 cut(s) 1968
PfeI GAWTC 5 cut(s) 817, 1135, 1379, 2308, 2577
PflMI CCANNNNNTGG 3 cut(s) 599, 632, 2564
PfoI TCCNGGA 1 cut(s) 613
PkrI GCNGC 7 cut(s) 115, 248, 449, 520, 738, 811, 1739
PleI GAGTC 6 cut(s) 604, 1264, 1427, 1801, 1847, 2207
PpsI GAGTC 6 cut(s) 604, 1264, 1427, 1801, 1847, 2207
PpuMI RGGWCCY 1 cut(s) 368
PscI ACATGT 1 cut(s) 1299
PsiI TTATAA 2 cut(s) 311, 1679
Psp124BI GAGCTC 2 cut(s) 54, 130
Psp5II RGGWCCY 1 cut(s) 368
Psp6I CCWGG 8 cut(s) 406, 547, 613, 1022, 1556, 2470, 2515, 2539
PspEI GGTNACC 1 cut(s) 2297
PspFI CCCAGC 1 cut(s) 199
PspGI CCWGG 8 cut(s) 406, 547, 613, 1022, 1556, 2470, 2515, 2539
PspN4I GGNNCC 4 cut(s) 205, 250, 369, 1450
PspPI GGNCC 6 cut(s) 103, 136, 368, 764, 1449, 1843
PspPPI RGGWCCY 1 cut(s) 368
PstI CTGCAG 1 cut(s) 1368
PstNI CAGNNNCTG 2 cut(s) 599, 1471
PsuI RGATCY 1 cut(s) 674
RsaI GTAC 2 cut(s) 363, 2323
RsaNI GTAC 2 cut(s) 362, 2322
RseI CAYNNNNRTG 1 cut(s) 1199
SacI GAGCTC 2 cut(s) 54, 130
SapI GCTCTTC 1 cut(s) 1932
SaqAI TTAA 7 cut(s) 14, 963, 1034, 1611, 2124, 2546, 2608
SatI GCNGC 7 cut(s) 114, 247, 448, 519, 737, 810, 1738
Sau3AI GATC 7 cut(s) 165, 617, 674, 919, 976, 1650, 1726
Sau96I GGNCC 6 cut(s) 103, 136, 368, 764, 1449, 1843
SchI GAGTC 6 cut(s) 605, 1264, 1428, 1802, 1847, 2208
SduI GDGCHC 5 cut(s) 44, 54, 130, 206, 2281
SfaNI GCATC 5 cut(s) 186, 772, 919, 1775, 2368
SfcI CTRYAG 2 cut(s) 1364, 1813
SfuI TTCGAA 1 cut(s) 380
SinI GGWCC 5 cut(s) 103, 368, 764, 1449, 1843
SmiMI CAYNNNNRTG 1 cut(s) 1199
SmlI CTYRAG 1 cut(s) 2123
SmoI CTYRAG 1 cut(s) 2123
SphI GCATGC 2 cut(s) 1710, 2093
SsiI CCGC 9 cut(s) 113, 246, 295, 712, 726, 1442, 2294, 2319, 2373
SspI AATATT 1 cut(s) 2137
SstI GAGCTC 2 cut(s) 54, 130
StyI CCWWGG 1 cut(s) 2302
TaiI ACGT 5 cut(s) 96, 404, 1601, 2044, 2327
TaqI TCGA 5 cut(s) 380, 949, 1133, 1488, 1729
TauI GCSGC 2 cut(s) 116, 249
TfiI GAWTC 5 cut(s) 817, 1135, 1379, 2308, 2577
Tru1I TTAA 7 cut(s) 14, 963, 1034, 1611, 2124, 2546, 2608
Tru9I TTAA 7 cut(s) 14, 963, 1034, 1611, 2124, 2546, 2608
TscAI CASTG 5 cut(s) 225, 439, 2215, 2528, 2578
TseFI GTSAC 4 cut(s) 402, 853, 1808, 2297
TseI GCWGC 5 cut(s) 447, 518, 736, 809, 1737
Tsp45I GTSAC 4 cut(s) 402, 853, 1808, 2297
TspGWI ACGGA 1 cut(s) 1786
TspRI CASTG 5 cut(s) 225, 439, 2215, 2528, 2578
Van91I CCANNNNNTGG 3 cut(s) 599, 632, 2564
Vha464I CTTAAG 1 cut(s) 2123
VneI GTGCAC 1 cut(s) 40
VpaK11BI GGWCC 5 cut(s) 103, 368, 764, 1449, 1843
XceI RCATGY 4 cut(s) 1303, 1710, 1809, 2093
XcmI CCANNNNNNNNNTGG 1 cut(s) 998
XmiI GTMKAC 1 cut(s) 752
XmnI GAANNNNTTC 1 cut(s) 1968
ZraI GACGTC 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.