pycom06g01230

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0009053
Physical Location & Seq
Reverse (-)
1294992 .. 1298356
3365 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g01230.1

Sequence Viewer

Length: 2880 bp
ATGTACTCACAATTTGGGATATATCCGGCATTTTCACAGAAGCTGTACCCTTTAAACCAAAGCCTTCTCACTTTCCCTCTCAAATTACACTTTCTGGCGCCTTCGTTTCGATTTCGATTCCGTCAGTTTTTTAGGGTTTCAGTTTTCACTCTCTATACACTCCAAAGCCCCAATCTTTGCACTATCTCTCTCTATTTTCCCAAATCAATCAACTTTCTCGGCTACCAAACAGCCAGTTACCCTGTTTCGACGATGGATGTGAGGTTAACAGAAGGGGCGATAATGAAGATGTGCACAGAGGAGCTTAACGACGGGAGCTTTCAGCCTATCCTGCAAGTGATGGACGTGAAGCTGGTCCATACGGCGGCGCGAAATGGAGCTCAGCCGGGCCAGGATAAGGAGAGATACAGGGTTGTGATCTCCGACGGGTCGCACTACCAGCAGGGGATGCTGGGCACCCAGAAGAACACACTGGTTCGGCAAGGTATGCTTCAGAGCGGCTCCATCGTCTGCCTCAAACAGTTCACCTGCAACGCAATCCAAAACCGCCTGATTATCATTATAATCGAATTGGATTTGGTGCTTGACAAATGTGATTTCATTGGCAGTCCTGTACCGGGACCTAAGTATTCGAATGCACAGTCGTCAGGGACGTCACCTGGAAATTCTCAATCTCTTGGTGGCACTGCACATTCTGGCGGCACAGTTCATCAAACTGGCACGGGGACTACTCCAGAACAGCCTAGAGTAAATCAATCATTGGGTAGTGCAGCTAGTGCTTACTCTGGTAATGCTGAGCCAGGAAGAAATGCTACTATGAATACACCTCCCAATTACCCTAAACCAGAGTCTGGATTTGGTTTTCCTGGATCAGCACCATCAAGTGGGTCATATGGCAATCAGAACATAGGTTTTCACAACCCTAGACCTGAGGTTCCTCAGCCACCACTAAATTCTTATGCCCGCCCACCTCAGCCATCTTATCAGCAGCCACCTCCGGTGTATACTAATAGAGGACCAGTTGCCAAAAATGATGCCCCTCCAAGAATAATGCCAATTGCTGCTCTGAATCCGTATCAAGGAAGGTGGACAATCAAGGCTAGAGTGACAGCTAAAGGAGAACTTAGGCGCTATAACAATACACGTGGCGATGGGAAAGTATTTTCTTTTGATCTTCTTGATGCTCATAATGGAGAAATTCGAGCAACCTGTTTTAATGCAGTGGCTGATCAATTCTACAACCAGATTGAAGTTGGTAAGATTTATTTGGTCTCCAGGGGTAGTTTAAAACCAGCTCAAAAGAATTTCAACCACCTCAACAATGACCATGAAATTCTTTTAGATAACTCATCAGTAATACAGTCATGTTTTGAGGATGATGATTCGATTCCACATCATAATTTCAATTTCAGTCCAATAAATCTTGTTGAAGGCTTGGAGAACAACACTATTGTGGATGTAATAGGTGTGGTATCATCCATCAGTCCCCCTGCCTCTATAATGAGGAAAAATGGTGTTGAGACTCAGAAGCGAACCCTCCACTTGAAGGACATGTCTGGTCGTAGTGTTGAAGTAACTCTTTGGGGAAATTTGTGCAATACAGAAGGACAAAGACTGCAGGTTATGTGTGATTCTGGTCTATTCCCGGTGCTTGCTGTAAAAGGTGGTAGAGTCAATGACTTCAATGGGAAGGCGGTGGGGACCGTTTCCACGAGTCAGCTTCTAATAGAGCCTAATATCGAGAAGGCTCATGAGGTGAGGAACTGGTTTGAGAAGGAAGGAAAGAATACCCCATGTATCTCTATTTCCAGGGAAACGGCGGGTGTGGGTAGAACAGAGAACCGAAAGACTATATCCCAAATTAAAGATGAGAAGCTAGGGACTTCCGAGAAGCCAGATTGGATCGCAGTTGGTGCAACCATTTCATTTGTAAAGGTTGATAGTTTTTGCTATTCAGCATGCCCAATCATGATTGGTGATCGACAATGCAGCAAAAAGGTTACAAATAATGGAGATGGGACTTGGAGGTGTGAAAGATGCAATGAGTCCGTGGAAGAATGTGACTACAGATATATACTCCAGTTTCAGATACAGGACCATACTGACTCAACATGGGTAACTGCATTTCAGGAGGGCGGTGAGGAAATCATGGGATTACCTGCAAAAGAATTGTATTTTATCAAATTTGAAGAGCAAGATGATGAGAAGTTTGCAGAAATCATTCAGAAGGTTCTCTTTACCAAATTCATTTTCAAGTTGAAAGTAAAGGAGGAGACTTTTAGTGATGAGCAACGTGTAAAATCAACAGTTATCAAAGCCGAGAAAGTGAACCATTCATCGCATGCTAGATATCATTTGGAATTGATGGATAAACTTAAGATGGAAAATATTAGTGCTTCTGCCTCCAAAGCTGACAATGTAACGCCTAGCGCTGGAATGAATGTTGCTGGTGTCGGGAACACTGCAATTAGACAGCCCACTCCTGCTGTGAATGTTGGTTACAGTAACAATGCTGGTAGAGAATTTGGAGCACCAGAAAATCAAGCGGGTCACCATGGGAATCAGTATGGCGGTACGTTCCTTTCAGGGGGTTCTGCTGGTTCATATCAAATATGTAAGAGTTGTGGGGATGCTGGCCATAGCTCAATGAACTGCCCAACTGTAATGAATGGCCCAGGACAGTCGTCAGGAGTGGGCTATAACAATAGAGTGTCTTCTAGGCCAGGTATCAGCAATACTTCTGGTGAATGCTACAAATGCCACCAACCTGGACACTGGGCAAGAGACTGTCCTGGGTTAAACACTTCTCCAGCTTATGGAAGCAGCGGAATCAACCGTGGGACATATGGTGTTTCAAATCAAGAGGTCGGTCGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

960

Amino Acids

105.18

Weight (kDa)

8.38

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 89 - 194 2.8e-24 Replication factor-A protein 1, N-terminal domain
tRNA_anti-codon PF01336 364 - 435 4.6e-09 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 474 - 576 5e-27 Replication protein A OB domain
Rep_fac-A_C PF08646 634 - 783 1.9e-52 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 563
AarI CACCTGC 1 cut(s) 536
AatII GACGTC 1 cut(s) 656
Acc36I ACCTGC 3 cut(s) 536, 1609, 2167
AccB1I GGYRCC 2 cut(s) 97, 455
AccB7I CCANNNNNTGG 3 cut(s) 851, 884, 2816
AccBSI CCGCTC 1 cut(s) 498
AccI GTMKAC 1 cut(s) 1004
AccII CGCG 1 cut(s) 370
AclWI GGATC 2 cut(s) 877, 1910
AcoI YGGCCR 1 cut(s) 2635
AcsI RAATTY 9 cut(s) 664, 952, 1197, 1303, 1332, 1588, 2183, 2243, 2522
AcuI CTGAAG 1 cut(s) 476
AcvI CACGTG 1 cut(s) 1145
AcyI GRCGYC 2 cut(s) 98, 653
AfaI GTAC 4 cut(s) 5, 47, 615, 2575
AfeI AGCGCT 1 cut(s) 2431
AflII CTTAAG 1 cut(s) 2375
AflIII ACRYGT 3 cut(s) 1142, 1551, 2293
AjiI CACGTC 1 cut(s) 346
AjuI GAANNNNNNNTTGG 2 cut(s) 2234, 2266
AleI CACNNNNGTG 1 cut(s) 1451
Alw21I GWGCWC 3 cut(s) 296, 382, 2533
Alw26I GTCTC 4 cut(s) 1276, 1514, 2267, 2778
Alw44I GTGCAC 1 cut(s) 292
AlwI GGATC 2 cut(s) 877, 1910
AlwNI CAGNNNCTG 3 cut(s) 43, 851, 1226
Aor51HI AGCGCT 1 cut(s) 2431
AoxI GGCC 4 cut(s) 388, 2635, 2671, 2720
ApaLI GTGCAC 1 cut(s) 292
ApeKI GCWGC 5 cut(s) 770, 988, 1061, 1989, 2823
ApoI RAATTY 9 cut(s) 664, 952, 1197, 1303, 1332, 1588, 2183, 2243, 2522
ArsI GACNNNNNNTTYG 2 cut(s) 626, 658
Asp700I GAANNNNTTC 1 cut(s) 2220
AspLEI GCGC 4 cut(s) 100, 370, 1131, 2432
AspS9I GGNCC 7 cut(s) 355, 388, 620, 1016, 1701, 2095, 2672
AsuC2I CCSGG 3 cut(s) 387, 618, 1646
AsuHPI GGTGA 7 cut(s) 517, 648, 1768, 1988, 2150, 2543, 2756
AsuII TTCGAA 1 cut(s) 632
AvaII GGWCC 5 cut(s) 355, 620, 1016, 1701, 2095
AxyI CCTNAGG 1 cut(s) 930
BaeGI GKGCMC 2 cut(s) 296, 458
BalI TGGCCA 1 cut(s) 2637
BanI GGYRCC 2 cut(s) 97, 455
BanII GRGCYC 1 cut(s) 382
BarI GAAGNNNNNNTAC 2 cut(s) 796, 828
BauI CACGAG 1 cut(s) 1711
BbrPI CACGTG 1 cut(s) 1145
BbsI GAAGAC 1 cut(s) 2706
Bbv12I GWGCWC 3 cut(s) 296, 382, 2533
BbvCI CCTCAGC 2 cut(s) 939, 972
BbvI GCAGC 5 cut(s) 782, 1000, 1048, 2001, 2835
BceAI ACGGC 2 cut(s) 378, 1833
BclI TGATCA 1 cut(s) 1228
BcnI CCSGG 3 cut(s) 387, 618, 1646
BcoDI GTCTC 4 cut(s) 1276, 1514, 2267, 2778
BfaI CTAG 8 cut(s) 744, 774, 924, 1101, 1877, 2346, 2427, 2718
BfmI CTRYAG 2 cut(s) 1616, 2065
BfoI RGCGCY 3 cut(s) 101, 1132, 2433
BfrI CTTAAG 1 cut(s) 2375
BfuAI ACCTGC 3 cut(s) 536, 1609, 2167
BisI GCNGC 8 cut(s) 366, 499, 700, 771, 989, 1062, 1990, 2824
BlpI GCTNAGC 2 cut(s) 381, 795
BlsI GCNGC 8 cut(s) 367, 500, 701, 772, 990, 1063, 1991, 2825
Bme18I GGWCC 5 cut(s) 355, 620, 1016, 1701, 2095
BmgBI CACGTC 1 cut(s) 346
BmgT120I GGNCC 7 cut(s) 355, 388, 620, 1016, 1701, 2095, 2672
BmiI GGNNCC 6 cut(s) 99, 457, 502, 621, 936, 1702
BmrI ACTGGG 1 cut(s) 2785
BmsI GCATC 5 cut(s) 438, 1024, 1171, 2027, 2620
BmuI ACTGGG 1 cut(s) 2785
BoxI GACNNNNGTC 1 cut(s) 2683
BpiI GAAGAC 1 cut(s) 2706
BpmI CTGGAG 4 cut(s) 717, 1258, 2063, 2793
Bpu10I CCTNAGC 2 cut(s) 939, 972
Bpu1102I GCTNAGC 2 cut(s) 381, 795
Bpu14I TTCGAA 1 cut(s) 632
BpuMI CCSGG 3 cut(s) 387, 618, 1646
BsaAI YACGTR 1 cut(s) 1145
BsaHI GRCGYC 2 cut(s) 98, 653
BsaI GGTCTC 1 cut(s) 1276
BsaJI CCNNGG 7 cut(s) 1275, 1809, 2049, 2554, 2674, 2792, 2836
BsaWI WCCGGW 1 cut(s) 997
Bse1I ACTGG 7 cut(s) 234, 477, 721, 1019, 1769, 2080, 2780
Bse21I CCTNAGG 1 cut(s) 930
Bse3DI GCAATG 1 cut(s) 2047
BseDI CCNNGG 7 cut(s) 1275, 1809, 2049, 2554, 2674, 2792, 2836
BseGI GGATG 6 cut(s) 262, 453, 1381, 1462, 1475, 2635
BseMI GCAATG 1 cut(s) 2047
BseMII CTCAG 6 cut(s) 395, 786, 921, 953, 986, 1538
BseNI ACTGG 7 cut(s) 234, 477, 721, 1019, 1769, 2080, 2780
BseRI GAGGAG 2 cut(s) 314, 2285
BseSI GKGCMC 2 cut(s) 296, 458
BseXI GCAGC 5 cut(s) 782, 1000, 1048, 2001, 2835
BseYI CCCAGC 1 cut(s) 451
BsgI GTGCAG 2 cut(s) 672, 789
Bsh1236I CGCG 1 cut(s) 370
Bsh1285I CGRYCG 1 cut(s) 2872
BshFI GGCC 4 cut(s) 390, 2637, 2673, 2722
BshNI GGYRCC 2 cut(s) 97, 455
BsiEI CGRYCG 1 cut(s) 2872
BsiHKAI GWGCWC 3 cut(s) 296, 382, 2533
BsiSI CCGG 5 cut(s) 26, 386, 617, 998, 1646
BslFI GGGAC 8 cut(s) 633, 664, 739, 1470, 1714, 1894, 2032, 2854
BsmAI GTCTC 4 cut(s) 1276, 1514, 2267, 2778
BsmFI GGGAC 8 cut(s) 633, 664, 739, 1470, 1714, 1894, 2032, 2854
BsmI GAATGC 2 cut(s) 640, 2753
BsnI GGCC 4 cut(s) 390, 2637, 2673, 2722
Bso31I GGTCTC 1 cut(s) 1276
Bsp119I TTCGAA 1 cut(s) 632
Bsp1286I GDGCHC 4 cut(s) 296, 382, 458, 2533
Bsp143I GATC 6 cut(s) 417, 869, 1171, 1228, 1902, 1978
Bsp1720I GCTNAGC 2 cut(s) 381, 795
Bsp19I CCATGG 1 cut(s) 2554
BspANI GGCC 4 cut(s) 390, 2637, 2673, 2722
BspCNI CTCAG 6 cut(s) 394, 787, 922, 952, 985, 1537
BspFNI CGCG 1 cut(s) 370
BspHI TCATGA 2 cut(s) 1750, 1968
BspLI GGNNCC 6 cut(s) 99, 457, 502, 621, 936, 1702
BspMAI CTGCAG 1 cut(s) 1620
BspMI ACCTGC 3 cut(s) 536, 1609, 2167
BspPI GGATC 2 cut(s) 877, 1910
BspQI GCTCTTC 1 cut(s) 2184
BspT104I TTCGAA 1 cut(s) 632
BspT107I GGYRCC 2 cut(s) 97, 455
BspTI CTTAAG 1 cut(s) 2375
BspTNI GGTCTC 1 cut(s) 1276
BsrBI CCGCTC 1 cut(s) 498
BsrDI GCAATG 1 cut(s) 2047
BsrI ACTGG 7 cut(s) 234, 477, 721, 1019, 1769, 2080, 2780
BssECI CCNNGG 7 cut(s) 1275, 1809, 2049, 2554, 2674, 2792, 2836
BssMI GATC 6 cut(s) 417, 869, 1171, 1228, 1902, 1978
BssNAI GTATAC 1 cut(s) 1005
BssNI GRCGYC 2 cut(s) 98, 653
BssSI CACGAG 1 cut(s) 1711
BssT1I CCWWGG 1 cut(s) 2554
Bst1107I GTATAC 1 cut(s) 1005
Bst2BI CACGAG 1 cut(s) 1711
Bst6I CTCTTC 1 cut(s) 2184
BstACI GRCGYC 2 cut(s) 98, 653
BstAFI CTTAAG 1 cut(s) 2375
BstAPI GCANNNNNTGC 4 cut(s) 448, 487, 776, 1913
BstBAI YACGTR 1 cut(s) 1145
BstBI TTCGAA 1 cut(s) 632
BstC8I GCNNGC 5 cut(s) 964, 1653, 1960, 2343, 2635
BstDEI CTNAG 8 cut(s) 381, 624, 795, 930, 939, 972, 1124, 1524
BstDSI CCRYGG 3 cut(s) 2049, 2554, 2836
BstEII GGTNACC 1 cut(s) 2549
BstF5I GGATG 6 cut(s) 262, 453, 1381, 1462, 1475, 2635
BstFNI CGCG 1 cut(s) 370
BstH2I RGCGCY 3 cut(s) 101, 1132, 2433
BstHHI GCGC 4 cut(s) 100, 370, 1131, 2432
BstKTI GATC 6 cut(s) 420, 872, 1174, 1231, 1905, 1981
BstMAI GTCTC 4 cut(s) 1276, 1514, 2267, 2778
BstMBI GATC 6 cut(s) 417, 869, 1171, 1228, 1902, 1978
BstMCI CGRYCG 1 cut(s) 2872
BstMWI GCNNNNNNNGC 8 cut(s) 331, 439, 448, 487, 776, 1913, 2408, 2757
BstNSI RCATGY 3 cut(s) 1555, 1962, 2345
BstPAI GACNNNNGTC 1 cut(s) 2683
BstPI GGTNACC 1 cut(s) 2549
BstSFI CTRYAG 2 cut(s) 1616, 2065
BstSLI GKGCMC 2 cut(s) 296, 458
BstUI CGCG 1 cut(s) 370
BstV1I GCAGC 5 cut(s) 782, 1000, 1048, 2001, 2835
BstV2I GAAGAC 1 cut(s) 2706
BstXI CCANNNNNNTGG 1 cut(s) 2768
BstZ17I GTATAC 1 cut(s) 1005
Bsu36I CCTNAGG 1 cut(s) 930
BsuRI GGCC 4 cut(s) 390, 2637, 2673, 2722
BtgI CCRYGG 3 cut(s) 2049, 2554, 2836
BtgZI GCGATG 2 cut(s) 1164, 2322
BtrI CACGTC 1 cut(s) 346
BtsCI GGATG 6 cut(s) 262, 453, 1381, 1462, 1475, 2635
BtsI GCAGTG 3 cut(s) 684, 1227, 2460
BtsIMutI CAGTG 5 cut(s) 470, 684, 1227, 2460, 2773
BveI ACCTGC 3 cut(s) 536, 1609, 2167
Cac8I GCNNGC 5 cut(s) 964, 1653, 1960, 2343, 2635
CaiI CAGNNNCTG 3 cut(s) 43, 851, 1226
CciI TCATGA 2 cut(s) 1750, 1968
CfoI GCGC 4 cut(s) 100, 370, 1131, 2432
Cfr13I GGNCC 7 cut(s) 355, 388, 620, 1016, 1701, 2095, 2672
Csp6I GTAC 4 cut(s) 4, 46, 614, 2574
CspCI CAANNNNNGTGG 4 cut(s) 1067, 1102, 1126, 1161
CviQI GTAC 4 cut(s) 4, 46, 614, 2574
DdeI CTNAG 8 cut(s) 381, 624, 795, 930, 939, 972, 1124, 1524
DinI GGCGCC 1 cut(s) 99
DpnI GATC 6 cut(s) 419, 871, 1173, 1230, 1904, 1980
DpnII GATC 6 cut(s) 417, 869, 1171, 1228, 1902, 1978
DraI TTTAAA 2 cut(s) 54, 1287
EaeI YGGCCR 1 cut(s) 2635
Eam1104I CTCTTC 1 cut(s) 2184
EarI CTCTTC 1 cut(s) 2184
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 2554
Eco24I GRGCYC 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 1276
Eco32I GATATC 1 cut(s) 2351
Eco47I GGWCC 5 cut(s) 355, 620, 1016, 1701, 2095
Eco47III AGCGCT 1 cut(s) 2431
Eco53kI GAGCTC 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 476
Eco72I CACGTG 1 cut(s) 1145
Eco81I CCTNAGG 1 cut(s) 930
Eco91I GGTNACC 1 cut(s) 2549
EcoICRI GAGCTC 1 cut(s) 380
EcoO109I RGGNCCY 1 cut(s) 620
EcoO65I GGTNACC 1 cut(s) 2549
EcoRV GATATC 1 cut(s) 2351
EcoT14I CCWWGG 1 cut(s) 2554
EcoT38I GRGCYC 1 cut(s) 382
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 2554
FalI AAGNNNNNCTT 8 cut(s) 474, 506, 1107, 1139, 1563, 1595, 2219, 2251
FaqI GGGAC 8 cut(s) 633, 664, 739, 1470, 1714, 1894, 2032, 2854
FauI CCCGC 3 cut(s) 971, 1813, 2539
FauNDI CATATG 2 cut(s) 892, 2845
FbaI TGATCA 1 cut(s) 1228
FblI GTMKAC 1 cut(s) 1004
Fnu4HI GCNGC 8 cut(s) 366, 499, 700, 771, 989, 1062, 1990, 2824
FokI GGATG 6 cut(s) 269, 460, 1388, 1462, 1469, 2642
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 8 cut(s) 366, 499, 700, 771, 989, 1062, 1990, 2824
FspBI CTAG 8 cut(s) 744, 774, 924, 1101, 1877, 2346, 2427, 2718
GlaI GCGC 4 cut(s) 99, 369, 1130, 2431
GluI GCNGC 8 cut(s) 366, 499, 700, 771, 989, 1062, 1990, 2824
GsaI CCCAGC 1 cut(s) 455
GsuI CTGGAG 4 cut(s) 717, 1258, 2063, 2793
HaeII RGCGCY 3 cut(s) 101, 1132, 2433
HaeIII GGCC 4 cut(s) 390, 2637, 2673, 2722
HapII CCGG 5 cut(s) 26, 386, 617, 998, 1646
HhaI GCGC 4 cut(s) 100, 370, 1131, 2432
Hin1I GRCGYC 2 cut(s) 98, 653
Hin6I GCGC 4 cut(s) 98, 368, 1129, 2430
HinP1I GCGC 4 cut(s) 98, 368, 1129, 2430
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HpaI GTTAAC 1 cut(s) 267
HpaII CCGG 5 cut(s) 26, 386, 617, 998, 1646
HphI GGTGA 7 cut(s) 517, 648, 1768, 1988, 2150, 2543, 2756
Hpy166II GTNNAC 6 cut(s) 267, 294, 525, 1005, 1089, 2329
Hpy188I TCNGA 8 cut(s) 424, 495, 903, 1068, 1527, 1888, 2088, 2226
Hpy8I GTNNAC 6 cut(s) 267, 294, 525, 1005, 1089, 2329
Hpy99I CGWCG 3 cut(s) 253, 314, 428
HpyCH4IV ACGT 5 cut(s) 345, 653, 1144, 2293, 2576
HpyF10VI GCNNNNNNNGC 8 cut(s) 331, 439, 448, 487, 776, 1913, 2408, 2757
HpyF3I CTNAG 8 cut(s) 381, 624, 795, 930, 939, 972, 1124, 1524
HpySE526I ACGT 5 cut(s) 345, 653, 1144, 2293, 2576
Hsp92I GRCGYC 2 cut(s) 98, 653
HspAI GCGC 4 cut(s) 98, 368, 1129, 2430
KasI GGCGCC 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 1228
KspAI GTTAAC 1 cut(s) 267
Kzo9I GATC 6 cut(s) 417, 869, 1171, 1228, 1902, 1978
LguI GCTCTTC 1 cut(s) 2184
LmnI GCTCC 5 cut(s) 301, 315, 377, 506, 2528
Lsp1109I GCAGC 5 cut(s) 782, 1000, 1048, 2001, 2835
LweI GCATC 5 cut(s) 438, 1024, 1171, 2027, 2620
MaeI CTAG 8 cut(s) 744, 774, 924, 1101, 1877, 2346, 2427, 2718
MaeII ACGT 5 cut(s) 345, 653, 1144, 2293, 2576
MalI GATC 6 cut(s) 419, 871, 1173, 1230, 1904, 1980
MbiI CCGCTC 1 cut(s) 498
MboI GATC 6 cut(s) 417, 869, 1171, 1228, 1902, 1978
MboII GAAGA 7 cut(s) 298, 475, 816, 1166, 2066, 2201, 2706
MfeI CAATTG 1 cut(s) 1056
MhlI GDGCHC 4 cut(s) 296, 382, 458, 2533
MlsI TGGCCA 1 cut(s) 2637
MluNI TGGCCA 1 cut(s) 2637
Mly113I GGCGCC 1 cut(s) 98
MlyI GAGTC 6 cut(s) 857, 1516, 1680, 1723, 2054, 2099
MmeI TCCRAC 1 cut(s) 447
Mox20I TGGCCA 1 cut(s) 2637
MroXI GAANNNNTTC 1 cut(s) 2220
MscI TGGCCA 1 cut(s) 2637
MseI TTAA 8 cut(s) 53, 266, 306, 1215, 1286, 1863, 2376, 2798
MslI CAYNNNNRTG 1 cut(s) 1451
Msp20I TGGCCA 1 cut(s) 2637
MspA1I CMGCKG 1 cut(s) 2826
MspCI CTTAAG 1 cut(s) 2375
MspI CCGG 5 cut(s) 26, 386, 617, 998, 1646
MunI CAATTG 1 cut(s) 1056
Mva1269I GAATGC 2 cut(s) 640, 2753
MvnI CGCG 1 cut(s) 370
MwoI GCNNNNNNNGC 8 cut(s) 331, 439, 448, 487, 776, 1913, 2408, 2757
NarI GGCGCC 1 cut(s) 98
NciI CCSGG 3 cut(s) 387, 618, 1646
NcoI CCATGG 1 cut(s) 2554
NdeI CATATG 2 cut(s) 892, 2845
NdeII GATC 6 cut(s) 417, 869, 1171, 1228, 1902, 1978
NlaIV GGNNCC 6 cut(s) 99, 457, 502, 621, 936, 1702
NmeAIII GCCGAG 2 cut(s) 198, 2344
NmuCI GTSAC 4 cut(s) 654, 1105, 2060, 2549
NspI RCATGY 3 cut(s) 1555, 1962, 2345
NspV TTCGAA 1 cut(s) 632
OliI CACNNNNGTG 1 cut(s) 1451
PaeI GCATGC 2 cut(s) 1962, 2345
PagI TCATGA 2 cut(s) 1750, 1968
PaqCI CACCTGC 1 cut(s) 536
PciI ACATGT 1 cut(s) 1551
PciSI GCTCTTC 1 cut(s) 2184
PcsI WCGNNNNNNNCGW 1 cut(s) 650
PctI GAATGC 2 cut(s) 640, 2753
PdmI GAANNNNTTC 1 cut(s) 2220
PfeI GAWTC 7 cut(s) 117, 1069, 1382, 1387, 1631, 2560, 2829
PflMI CCANNNNNTGG 3 cut(s) 851, 884, 2816
PfoI TCCNGGA 1 cut(s) 865
PkrI GCNGC 8 cut(s) 367, 500, 701, 772, 990, 1063, 1991, 2825
PleI GAGTC 6 cut(s) 856, 1516, 1679, 1722, 2053, 2099
PluTI GGCGCC 1 cut(s) 101
PmaCI CACGTG 1 cut(s) 1145
PmlI CACGTG 1 cut(s) 1145
PpsI GAGTC 6 cut(s) 856, 1516, 1679, 1722, 2053, 2099
Ppu21I YACGTR 1 cut(s) 1145
PpuMI RGGWCCY 1 cut(s) 620
PscI ACATGT 1 cut(s) 1551
PshAI GACNNNNGTC 1 cut(s) 2683
PsiI TTATAA 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 382
Psp5II RGGWCCY 1 cut(s) 620
PspCI CACGTG 1 cut(s) 1145
PspEI GGTNACC 1 cut(s) 2549
PspFI CCCAGC 1 cut(s) 451
PspN4I GGNNCC 6 cut(s) 99, 457, 502, 621, 936, 1702
PspPI GGNCC 7 cut(s) 355, 388, 620, 1016, 1701, 2095, 2672
PspPPI RGGWCCY 1 cut(s) 620
PstI CTGCAG 1 cut(s) 1620
PstNI CAGNNNCTG 3 cut(s) 43, 851, 1226
RsaI GTAC 4 cut(s) 5, 47, 615, 2575
RsaNI GTAC 4 cut(s) 4, 46, 614, 2574
RseI CAYNNNNRTG 1 cut(s) 1451
SacI GAGCTC 1 cut(s) 382
SapI GCTCTTC 1 cut(s) 2184
SaqAI TTAA 8 cut(s) 53, 266, 306, 1215, 1286, 1863, 2376, 2798
SatI GCNGC 8 cut(s) 366, 499, 700, 771, 989, 1062, 1990, 2824
Sau3AI GATC 6 cut(s) 417, 869, 1171, 1228, 1902, 1978
Sau96I GGNCC 7 cut(s) 355, 388, 620, 1016, 1701, 2095, 2672
SchI GAGTC 6 cut(s) 857, 1516, 1680, 1723, 2054, 2099
SduI GDGCHC 4 cut(s) 296, 382, 458, 2533
SfaNI GCATC 5 cut(s) 438, 1024, 1171, 2027, 2620
SfcI CTRYAG 2 cut(s) 1616, 2065
SfoI GGCGCC 1 cut(s) 99
SfuI TTCGAA 1 cut(s) 632
SinI GGWCC 5 cut(s) 355, 620, 1016, 1701, 2095
SmiMI CAYNNNNRTG 1 cut(s) 1451
SmlI CTYRAG 1 cut(s) 2375
SmoI CTYRAG 1 cut(s) 2375
SphI GCATGC 2 cut(s) 1962, 2345
SspDI GGCGCC 1 cut(s) 97
SspI AATATT 1 cut(s) 2389
SspMI CTAG 8 cut(s) 744, 774, 924, 1101, 1877, 2346, 2427, 2718
SstI GAGCTC 1 cut(s) 382
StyI CCWWGG 1 cut(s) 2554
TaiI ACGT 5 cut(s) 348, 656, 1147, 2296, 2579
TaqI TCGA 9 cut(s) 109, 115, 248, 567, 632, 1201, 1385, 1740, 1981
TaqII GACCGA 1 cut(s) 2858
TatI WGTACW 1 cut(s) 3
TauI GCSGC 3 cut(s) 368, 501, 702
TfiI GAWTC 7 cut(s) 117, 1069, 1382, 1387, 1631, 2560, 2829
Tru1I TTAA 8 cut(s) 53, 266, 306, 1215, 1286, 1863, 2376, 2798
Tru9I TTAA 8 cut(s) 53, 266, 306, 1215, 1286, 1863, 2376, 2798
TscAI CASTG 5 cut(s) 477, 691, 1227, 2467, 2780
TseFI GTSAC 4 cut(s) 654, 1105, 2060, 2549
TseI GCWGC 5 cut(s) 770, 988, 1061, 1989, 2823
Tsp45I GTSAC 4 cut(s) 654, 1105, 2060, 2549
TspGWI ACGGA 3 cut(s) 110, 1062, 2038
TspRI CASTG 5 cut(s) 477, 691, 1227, 2467, 2780
Van91I CCANNNNNTGG 3 cut(s) 851, 884, 2816
Vha464I CTTAAG 1 cut(s) 2375
VneI GTGCAC 1 cut(s) 292
VpaK11BI GGWCC 5 cut(s) 355, 620, 1016, 1701, 2095
XapI RAATTY 9 cut(s) 664, 952, 1197, 1303, 1332, 1588, 2183, 2243, 2522
XceI RCATGY 3 cut(s) 1555, 1962, 2345
XcmI CCANNNNNNNNNTGG 1 cut(s) 1250
XmiI GTMKAC 1 cut(s) 1004
XmnI GAANNNNTTC 1 cut(s) 2220
XspI CTAG 8 cut(s) 744, 774, 924, 1101, 1877, 2346, 2427, 2718
ZraI GACGTC 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.