FvH4_5g23941

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009053
Physical Location & Seq
Reverse (-)
15234777 .. 15238569
3793 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23941.t1

Sequence Viewer

Length: 2625 bp
ATGGCCGTTAGGCTAACGGAGGGCGCGGTACTGAAGATGACGGCGGAGGACGCCGATCAATCCTTCAAGCCCGTCCTGCAAGTCACCGACCTGAGACAGATCCACACACAGCGCAGCGGCGGCGACTCAGAACGGTACAGGCTTAACTTGTCCGATGGGTCCCACTTGGAGCAGGGGATGCTTGCCAGTCAGCATAACTCCCTGGTTCACCAAGGCCTCCTTCAGAAGGGCTCCATCGTCTGCCTCACTGAATTCACCTGCACCAAAGTCCAGAATCGCAGGATCATCATTATAGTCTCACTGGATGTGCTAGTTAGTGAATGTGATATCATTGGACAGCCTGTCAGATTGTCGAATGCGCAGTCCCCAGCGACAATACCTGGAAACACCCAATCTCTTAACAGCAGTGTGCGTGGGGCGGGTCATGAAGCTATGACTGAGGCGAGTCCCGATCAACCTAGATTGAATCCATCGTATGGCGGTTCTTACTCTGGTAGGGCTGAACCAGGGAGATATGCTGCAACGAGCTCAGTTCCTAATCACCCTAGACCTGAGAGTGGTTCTGGTTTTGCTGGGTCAGCACCTGGGTCATATGCTGGTCAGAACACGGTTTTACGCAGCCCAAGACCCCAGGTTTCACCTCCACTCCAAAGTTCTTATGCTCACCCACCTCAGCCGACTTATCAACAGTCGCGCTCAAATTCTTATGCCCAACAGCCTCAGTCAACTTATCAACAGGCCCCTCCAAGTTCTTATGCACGCTCACCACAGCCAACTCATCAACAGCCACCTCCATTGTACAGTAACAGAGGACCAGTAGCCAAAAATGAAGCTCCTGGTAGAATAGTACAAATTTGTGCTCTGAATATGTACCAAAATAGGTGGACAATCAAGGCGAGAGTGACAGCTAAATCAGAACTCAGGACCTACACCAATGCACGTGGTGAGGGGAAAGTATTTAATTTTGATCTTATTGATTCCAGTGGAGAAATTAGAGTAACCTGCTTTAATCCTGTGGCTGAAGAATTCTACAGTTTGATTGAAGTTGGTAAGGTGTATCGTATCTCGAAAGGTACCATCAAGACGGCTGCGCGGAAATTTAACCACTTGAATAATGAGTTTGAAATTCATTTGGACGCCACTTCGACAATCCAATTATGTTTTGAGGATGATGGCTCGATTCCACAGCAGCGATTCAATTTTCGGCCAATAAGTGATGTTGAAGGCTTGGAGAACAACACTATTGTGGACATAATAGGTGTGGTGTCATTCATCAGTCCAGCTGCTTCAATAAGGACGAAAAATGGTGAGACTCAAAAGAGAACTCTCCAGTTGAAGGATATGTCTTGTCGTAGCGTGGAAGTAACTCTGTGGGGGAATTTGTGTAACGTAGAAGGTCAAAAACTTCAAGGTATGTGCGATTCTGGGTTGTTTCCCGTGGTAGCTGTCAAAGCTGGTAGAGTCAGTGAGTTCAACGGCAAGGTGGTGGGGACCACGGCTGCTAGTCAGCTCTTTATAGAACCAAAGGACTCTCAAGCTGAAGAGGTGAGGTTATGGTTTGAGAGGGAAGGAATGGATTCTCCATGTGTCTCTATCTCCAGGGACACAGCAAGAAATGACATCAGAAAGACTATATCCCAGATTAAAGATGAGAAGCTAGGCACTTTTGAGAAGCCAGATTGGATCACAGTCGGTGCAACTGTCACATTTATAAAGAGTGATAACTGTTTCTATCCGGCATGCCCACTCAAAACAGGGGACCGGCAATGCAACAAAAAGGTTATAAATGATGGAGATGGGACATGGAGGTGTGAAAGATGTGATCAGTCAGTACAACAGTGTGACTACAGATATATACTCAACTTACAGATACAAGACCATACTGGCGAAGCATGGGTAACTGCATTTCAGGAGAGTGGCGAAGAAATAATGGGTATAACAGCAAAAGAATTTCATTATTTGAAACATGAAGTGCAGGATGAGGATAAAGTTGCAGAAATCATTCGAAGGGTCTTGTTTACTGAATTTGCTTTCAAATTGAAAATAAAGGAAGAGACTTTTAGTGATGAGCAGCGTGTGAAATCAACTGTCGTCAAAGCAGATAAAGTGAATTTTTCTACGAAGTCTAGATTTCAGTTGGATCTGATCGATAACCTTAAAAAAGGGGAAACTGCAATACCTTGTGCTGGGATGAATAATTCTGGAGTGGGGAGCTCTGCAATTGGACAACATACACCTGCTGTCAATGTTAGTTACAGTACCAATGCCGCTAATGCTAGTAGTGTGTTTGGAGCACCAGCAAGTCAATTGGGACACGTTGGAAGCCAGTATATTAGTCCTAGAGTTCCTCCAACTGGCGGCTCTGCTGGTTCGTATCTTAGCTGTAACATCTGTGGAGGTACAAGCCATAATTCTTTGAACTGCCCAAATGTTATGAAACAATCATCAGGAGGGAGCTATGCCAATAGTAGGCCAGTTGCCAGTAATTCTGGTGAATGCTATAAATGCCATCAACCTGGTCATTGGGCGAGCGACTGCCCTGTCTCGAACCATATTCCAGCTTATGGAAGTAGTGGAGTCCAGCCTGGGAGATATGGAGTTTCAAATCAAAGGGTTAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

875

Amino Acids

95.59

Weight (kDa)

8.2

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 106 2.1e-27 Replication factor-A protein 1, N-terminal domain
tRNA_anti-codon PF01336 295 - 378 8.7e-12 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 404 - 506 2.8e-25 Replication protein A OB domain
Rep_fac-A_C PF08646 561 - 710 4.1e-53 Replication factor-A C terminal domain
zf-CCHC PF00098 832 - 847 9.8e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1712, 1784
AarI CACCTGC 2 cut(s) 266, 2245
AasI GACNNNNNNGTC 1 cut(s) 2540
Acc16I TGCGCA 1 cut(s) 360
Acc36I ACCTGC 3 cut(s) 266, 1010, 2245
Acc65I GGTACC 1 cut(s) 1073
AccB1I GGYRCC 1 cut(s) 1073
AccB7I CCANNNNNTGG 2 cut(s) 476, 2564
AccII CGCG 3 cut(s) 26, 694, 1093
AciI CCGC 9 cut(s) 26, 44, 117, 120, 419, 480, 1093, 2268, 2358
AclWI GGATC 4 cut(s) 94, 290, 1691, 2148
AcoI YGGCCR 2 cut(s) 3, 1205
AcuI CTGAAG 4 cut(s) 53, 206, 1041, 1560
AcvI CACGTG 1 cut(s) 941
AcyI GRCGYC 2 cut(s) 51, 1137
AdeI CACNNNGTG 1 cut(s) 944
AfaI GTAC 9 cut(s) 30, 137, 800, 849, 872, 1075, 1833, 2260, 2401
AfiI CCNNNNNNNGG 9 cut(s) 226, 476, 557, 1336, 2186, 2354, 2357, 2469, 2564
AflIII ACRYGT 1 cut(s) 2314
AhdI GACNNNNNGTC 1 cut(s) 341
AjnI CCWGG 9 cut(s) 201, 379, 505, 583, 630, 835, 1598, 2515, 2584
AjuI GAANNNNNNNTTGG 2 cut(s) 204, 236
AleI CACNNNNGTG 1 cut(s) 1244
Alw21I GWGCWC 4 cut(s) 530, 862, 2216, 2296
Alw26I GTCTC 6 cut(s) 88, 301, 1304, 1594, 2048, 2548
AlwI GGATC 4 cut(s) 94, 290, 1691, 2148
AlwNI CAGNNNCTG 1 cut(s) 584
AoxI GGCC 5 cut(s) 3, 214, 738, 1205, 2471
ApeKI GCWGC 8 cut(s) 114, 518, 618, 1088, 1189, 1283, 1499, 2071
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
Asp700I GAANNNNTTC 2 cut(s) 1576, 2001
Asp718I GGTACC 1 cut(s) 1073
AspLEI GCGC 5 cut(s) 26, 114, 361, 696, 1093
AspS9I GGNCC 6 cut(s) 159, 739, 812, 924, 1491, 1759
AsuII TTCGAA 1 cut(s) 2005
AvaII GGWCC 5 cut(s) 159, 812, 924, 1491, 1759
BanI GGYRCC 1 cut(s) 1073
BanII GRGCYC 3 cut(s) 233, 530, 2216
BbrPI CACGTG 1 cut(s) 941
Bbv12I GWGCWC 4 cut(s) 530, 862, 2216, 2296
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 8 cut(s) 126, 505, 630, 1075, 1201, 1270, 1486, 2083
BccI CCATC 8 cut(s) 149, 242, 478, 1085, 1166, 1784, 1790, 2517
BceAI ACGGC 4 cut(s) 57, 1101, 1492, 1512
BciT130I CCWGG 9 cut(s) 203, 381, 507, 585, 632, 837, 1600, 2517, 2586
BclI TGATCA 1 cut(s) 1822
BcoDI GTCTC 6 cut(s) 88, 301, 1304, 1594, 2048, 2548
BfaI CTAG 8 cut(s) 311, 459, 546, 1503, 1658, 2127, 2277, 2340
BfmI CTRYAG 2 cut(s) 1030, 1846
BfuAI ACCTGC 3 cut(s) 266, 1010, 2245
Bme1390I CCNGG 9 cut(s) 203, 381, 507, 585, 632, 837, 1600, 2517, 2586
Bme18I GGWCC 5 cut(s) 159, 812, 924, 1491, 1759
BmeRI GACNNNNNGTC 1 cut(s) 341
BmgT120I GGNCC 6 cut(s) 159, 739, 812, 924, 1491, 1759
BmiI GGNNCC 7 cut(s) 160, 161, 232, 741, 1075, 1492, 1760
BmrFI CCNGG 9 cut(s) 203, 381, 507, 585, 632, 837, 1600, 2517, 2586
BmsI GCATC 1 cut(s) 168
BpmI CTGGAG 3 cut(s) 1313, 1582, 2223
Bpu10I CCTNAGC 1 cut(s) 672
Bpu14I TTCGAA 1 cut(s) 2005
BpuEI CTTGAG 1 cut(s) 1518
Bsa29I ATCGAT 1 cut(s) 2148
BsaAI YACGTR 1 cut(s) 941
BsaHI GRCGYC 2 cut(s) 51, 1137
BsaJI CCNNGG 9 cut(s) 201, 211, 506, 584, 630, 1437, 1494, 1599, 2585
BsaXI ACNNNNNCTCC 2 cut(s) 630, 660
Bsc4I CCNNNNNNNGG 9 cut(s) 226, 476, 557, 1336, 2186, 2354, 2357, 2469, 2564
Bse118I RCCGGY 1 cut(s) 1761
Bse3DI GCAATG 1 cut(s) 1772
BseBI CCWGG 9 cut(s) 203, 381, 507, 585, 632, 837, 1600, 2517, 2586
BseCI ATCGAT 1 cut(s) 2148
BseDI CCNNGG 9 cut(s) 201, 211, 506, 584, 630, 1437, 1494, 1599, 2585
BseGI GGATG 5 cut(s) 183, 310, 1174, 1984, 2196
BseLI CCNNNNNNNGG 9 cut(s) 226, 476, 557, 1336, 2186, 2354, 2357, 2469, 2564
BseMI GCAATG 1 cut(s) 1772
BseMII CTCAG 8 cut(s) 83, 141, 429, 543, 543, 686, 734, 934
BseXI GCAGC 8 cut(s) 126, 505, 630, 1075, 1201, 1270, 1486, 2083
BseYI CCCAGC 3 cut(s) 367, 572, 2186
BsgI GTGCAG 2 cut(s) 244, 1994
Bsh1236I CGCG 3 cut(s) 26, 694, 1093
BshFI GGCC 5 cut(s) 5, 216, 740, 1207, 2473
BshNI GGYRCC 1 cut(s) 1073
BshVI ATCGAT 1 cut(s) 2148
BsiHKAI GWGCWC 4 cut(s) 530, 862, 2216, 2296
BsiSI CCGG 2 cut(s) 1736, 1762
BslFI GGGAC 8 cut(s) 145, 349, 432, 1504, 1616, 1772, 1813, 2325
BslI CCNNNNNNNGG 9 cut(s) 226, 476, 557, 1336, 2186, 2354, 2357, 2469, 2564
BsmAI GTCTC 6 cut(s) 88, 301, 1304, 1594, 2048, 2548
BsmFI GGGAC 8 cut(s) 145, 349, 432, 1504, 1616, 1772, 1813, 2325
BsmI GAATGC 2 cut(s) 361, 2501
BsnI GGCC 5 cut(s) 5, 216, 740, 1207, 2473
Bsp119I TTCGAA 1 cut(s) 2005
Bsp1286I GDGCHC 5 cut(s) 233, 530, 862, 2216, 2296
Bsp1407I TGTACA 1 cut(s) 798
Bsp143I GATC 9 cut(s) 55, 99, 282, 451, 967, 1683, 1822, 2140, 2145
BspACI CCGC 9 cut(s) 26, 44, 117, 120, 419, 480, 1093, 2268, 2358
BspANI GGCC 5 cut(s) 5, 216, 740, 1207, 2473
BspCNI CTCAG 8 cut(s) 84, 140, 430, 542, 544, 685, 733, 933
BspDI ATCGAT 1 cut(s) 2148
BspFNI CGCG 3 cut(s) 26, 694, 1093
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 7 cut(s) 160, 161, 232, 741, 1075, 1492, 1760
BspMI ACCTGC 3 cut(s) 266, 1010, 2245
BspPI GGATC 4 cut(s) 94, 290, 1691, 2148
BspT104I TTCGAA 1 cut(s) 2005
BspT107I GGYRCC 1 cut(s) 1073
BsrDI GCAATG 1 cut(s) 1772
BsrFI RCCGGY 1 cut(s) 1761
BsrGI TGTACA 1 cut(s) 798
BssAI RCCGGY 1 cut(s) 1761
BssECI CCNNGG 9 cut(s) 201, 211, 506, 584, 630, 1437, 1494, 1599, 2585
BssMI GATC 9 cut(s) 55, 99, 282, 451, 967, 1683, 1822, 2140, 2145
BssNI GRCGYC 2 cut(s) 51, 1137
BssT1I CCWWGG 1 cut(s) 211
Bst2UI CCWGG 9 cut(s) 203, 381, 507, 585, 632, 837, 1600, 2517, 2586
Bst6I CTCTTC 2 cut(s) 1536, 2046
BstACI GRCGYC 2 cut(s) 51, 1137
BstAPI GCANNNNNTGC 1 cut(s) 178
BstAUI TGTACA 1 cut(s) 798
BstBAI YACGTR 1 cut(s) 941
BstBI TTCGAA 1 cut(s) 2005
BstC8I GCNNGC 4 cut(s) 183, 760, 1741, 2530
BstDEI CTNAG 9 cut(s) 92, 127, 438, 529, 552, 672, 720, 920, 2378
BstDSI CCRYGG 2 cut(s) 1437, 1494
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 5 cut(s) 183, 310, 1174, 1984, 2196
BstFNI CGCG 3 cut(s) 26, 694, 1093
BstHHI GCGC 5 cut(s) 26, 114, 361, 696, 1093
BstKTI GATC 9 cut(s) 58, 102, 285, 454, 970, 1686, 1825, 2143, 2148
BstMAI GTCTC 6 cut(s) 88, 301, 1304, 1594, 2048, 2548
BstMBI GATC 9 cut(s) 55, 99, 282, 451, 967, 1683, 1822, 2140, 2145
BstMWI GCNNNNNNNGC 8 cut(s) 50, 76, 120, 178, 578, 1451, 2273, 2505
BstNI CCWGG 9 cut(s) 203, 381, 507, 585, 632, 837, 1600, 2517, 2586
BstNSI RCATGY 1 cut(s) 1743
BstSCI CCNGG 9 cut(s) 201, 379, 505, 583, 630, 835, 1598, 2515, 2584
BstSFI CTRYAG 2 cut(s) 1030, 1846
BstUI CGCG 3 cut(s) 26, 694, 1093
BstV1I GCAGC 8 cut(s) 126, 505, 630, 1075, 1201, 1270, 1486, 2083
BstX2I RGATCY 2 cut(s) 99, 2140
BstXI CCANNNNNNTGG 1 cut(s) 2516
BstYI RGATCY 2 cut(s) 99, 2140
Bsu15I ATCGAT 1 cut(s) 2148
BsuRI GGCC 5 cut(s) 5, 216, 740, 1207, 2473
BsuTUI ATCGAT 1 cut(s) 2148
BtgI CCRYGG 2 cut(s) 1437, 1494
BtsCI GGATG 5 cut(s) 183, 310, 1174, 1984, 2196
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 6 cut(s) 246, 299, 412, 988, 1471, 1844
BveI ACCTGC 3 cut(s) 266, 1010, 2245
Cac8I GCNNGC 4 cut(s) 183, 760, 1741, 2530
CaiI CAGNNNCTG 1 cut(s) 584
CciI TCATGA 1 cut(s) 424
CfoI GCGC 5 cut(s) 26, 114, 361, 696, 1093
Cfr10I RCCGGY 1 cut(s) 1761
Cfr13I GGNCC 6 cut(s) 159, 739, 812, 924, 1491, 1759
ClaI ATCGAT 1 cut(s) 2148
CseI GACGC 2 cut(s) 59, 1145
CsiI ACCWGGT 1 cut(s) 2515
Csp6I GTAC 9 cut(s) 29, 136, 799, 848, 871, 1074, 1832, 2259, 2400
CspCI CAANNNNNGTGG 6 cut(s) 777, 812, 863, 898, 922, 957
CviAII CATG 6 cut(s) 425, 1584, 1740, 1803, 1893, 1967
CviQI GTAC 9 cut(s) 29, 136, 799, 848, 871, 1074, 1832, 2259, 2400
DdeI CTNAG 9 cut(s) 92, 127, 438, 529, 552, 672, 720, 920, 2378
DpnI GATC 9 cut(s) 57, 101, 284, 453, 969, 1685, 1824, 2142, 2147
DpnII GATC 9 cut(s) 55, 99, 282, 451, 967, 1683, 1822, 2140, 2145
DraIII CACNNNGTG 1 cut(s) 944
DrdI GACNNNNNNGTC 1 cut(s) 2540
DriI GACNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 2540
EaeI YGGCCR 2 cut(s) 3, 1205
Eam1104I CTCTTC 2 cut(s) 1536, 2046
Eam1105I GACNNNNNGTC 1 cut(s) 341
EarI CTCTTC 2 cut(s) 1536, 2046
EciI GGCGGA 1 cut(s) 59
Ecl136II GAGCTC 2 cut(s) 528, 2214
Eco130I CCWWGG 1 cut(s) 211
Eco147I AGGCCT 1 cut(s) 216
Eco24I GRGCYC 3 cut(s) 233, 530, 2216
Eco32I GATATC 1 cut(s) 328
Eco47I GGWCC 5 cut(s) 159, 812, 924, 1491, 1759
Eco53kI GAGCTC 2 cut(s) 528, 2214
Eco57I CTGAAG 4 cut(s) 53, 206, 1041, 1560
Eco72I CACGTG 1 cut(s) 941
EcoICRI GAGCTC 2 cut(s) 528, 2214
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoO109I RGGNCCY 3 cut(s) 159, 739, 924
EcoRI GAATTC 2 cut(s) 251, 1025
EcoRII CCWGG 9 cut(s) 201, 379, 505, 583, 630, 835, 1598, 2515, 2584
EcoRV GATATC 1 cut(s) 328
EcoT14I CCWWGG 1 cut(s) 211
EcoT38I GRGCYC 3 cut(s) 233, 530, 2216
ErhI CCWWGG 1 cut(s) 211
FaeI CATG 6 cut(s) 428, 1587, 1743, 1806, 1896, 1970
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 8 cut(s) 145, 349, 432, 1504, 1616, 1772, 1813, 2325
FatI CATG 6 cut(s) 424, 1583, 1739, 1802, 1892, 1966
FauI CCCGC 1 cut(s) 412
FauNDI CATATG 1 cut(s) 592
FbaI TGATCA 1 cut(s) 1822
FokI GGATG 5 cut(s) 190, 317, 1181, 1991, 2203
FriOI GRGCYC 3 cut(s) 233, 530, 2216
FspBI CTAG 8 cut(s) 311, 459, 546, 1503, 1658, 2127, 2277, 2340
FspI TGCGCA 1 cut(s) 360
GlaI GCGC 5 cut(s) 25, 113, 360, 695, 1092
GsaI CCCAGC 3 cut(s) 371, 576, 2190
GsuI CTGGAG 3 cut(s) 1313, 1582, 2223
HaeIII GGCC 5 cut(s) 5, 216, 740, 1207, 2473
HapII CCGG 2 cut(s) 1736, 1762
HgaI GACGC 2 cut(s) 59, 1145
HhaI GCGC 5 cut(s) 26, 114, 361, 696, 1093
Hin1I GRCGYC 2 cut(s) 51, 1137
Hin1II CATG 6 cut(s) 428, 1587, 1743, 1806, 1896, 1970
Hin6I GCGC 5 cut(s) 24, 112, 359, 694, 1091
HinP1I GCGC 5 cut(s) 24, 112, 359, 694, 1091
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HpaII CCGG 2 cut(s) 1736, 1762
Hpy166II GTNNAC 5 cut(s) 208, 726, 885, 1249, 2019
Hpy188I TCNGA 9 cut(s) 130, 154, 225, 347, 603, 864, 916, 1625, 2145
Hpy8I GTNNAC 5 cut(s) 208, 726, 885, 1249, 2019
HpyAV CCTTC 8 cut(s) 73, 220, 230, 1217, 1330, 1388, 1562, 2001
HpyCH4IV ACGT 3 cut(s) 940, 1389, 2316
HpyF10VI GCNNNNNNNGC 8 cut(s) 50, 76, 120, 178, 578, 1451, 2273, 2505
HpyF3I CTNAG 9 cut(s) 92, 127, 438, 529, 552, 672, 720, 920, 2378
HpySE526I ACGT 3 cut(s) 940, 1389, 2316
Hsp92I GRCGYC 2 cut(s) 51, 1137
Hsp92II CATG 6 cut(s) 428, 1587, 1743, 1806, 1896, 1970
HspAI GCGC 5 cut(s) 24, 112, 359, 694, 1091
KflI GGGWCCC 1 cut(s) 159
KpnI GGTACC 1 cut(s) 1077
Ksp22I TGATCA 1 cut(s) 1822
Kzo9I GATC 9 cut(s) 55, 99, 282, 451, 967, 1683, 1822, 2140, 2145
LmnI GCTCC 6 cut(s) 169, 236, 838, 2211, 2291, 2454
Lsp1109I GCAGC 8 cut(s) 126, 505, 630, 1075, 1201, 1270, 1486, 2083
LweI GCATC 1 cut(s) 168
MabI ACCWGGT 1 cut(s) 2515
MaeI CTAG 8 cut(s) 311, 459, 546, 1503, 1658, 2127, 2277, 2340
MaeII ACGT 3 cut(s) 940, 1389, 2316
MalI GATC 9 cut(s) 57, 101, 284, 453, 969, 1685, 1824, 2142, 2147
MboI GATC 9 cut(s) 55, 99, 282, 451, 967, 1683, 1822, 2140, 2145
MboII GAAGA 5 cut(s) 46, 1034, 1553, 1934, 2063
MfeI CAATTG 2 cut(s) 2220, 2306
MflI RGATCY 2 cut(s) 99, 2140
MhlI GDGCHC 5 cut(s) 233, 530, 862, 2216, 2296
MlyI GAGTC 6 cut(s) 119, 454, 1306, 1470, 1523, 2586
MmeI TCCRAC 3 cut(s) 2118, 2299, 2375
MroXI GAANNNNTTC 2 cut(s) 1576, 2001
MseI TTAA 7 cut(s) 144, 399, 960, 1008, 1101, 1644, 2157
MslI CAYNNNNRTG 2 cut(s) 1244, 1807
MspA1I CMGCKG 2 cut(s) 117, 1283
MspI CCGG 2 cut(s) 1736, 1762
MspR9I CCNGG 9 cut(s) 203, 381, 507, 585, 632, 837, 1600, 2517, 2586
MunI CAATTG 2 cut(s) 2220, 2306
Mva1269I GAATGC 2 cut(s) 361, 2501
MvaI CCWGG 9 cut(s) 203, 381, 507, 585, 632, 837, 1600, 2517, 2586
MvnI CGCG 3 cut(s) 26, 694, 1093
MwoI GCNNNNNNNGC 8 cut(s) 50, 76, 120, 178, 578, 1451, 2273, 2505
NdeI CATATG 1 cut(s) 592
NdeII GATC 9 cut(s) 55, 99, 282, 451, 967, 1683, 1822, 2140, 2145
NlaIII CATG 6 cut(s) 428, 1587, 1743, 1806, 1896, 1970
NlaIV GGNNCC 7 cut(s) 160, 161, 232, 741, 1075, 1492, 1760
NmuCI GTSAC 4 cut(s) 82, 901, 1702, 1841
NsbI TGCGCA 1 cut(s) 360
NspI RCATGY 1 cut(s) 1743
NspV TTCGAA 1 cut(s) 2005
OliI CACNNNNGTG 1 cut(s) 1244
PaeI GCATGC 1 cut(s) 1743
PagI TCATGA 1 cut(s) 424
PaqCI CACCTGC 2 cut(s) 266, 2245
PceI AGGCCT 1 cut(s) 216
PctI GAATGC 2 cut(s) 361, 2501
PdmI GAANNNNTTC 2 cut(s) 1576, 2001
PfeI GAWTC 7 cut(s) 274, 466, 977, 1180, 1194, 1421, 1577
PflMI CCANNNNNTGG 2 cut(s) 476, 2564
PleI GAGTC 6 cut(s) 119, 453, 1306, 1469, 1523, 2585
PmaCI CACGTG 1 cut(s) 941
PmlI CACGTG 1 cut(s) 941
PpsI GAGTC 6 cut(s) 119, 453, 1306, 1469, 1523, 2585
Ppu21I YACGTR 1 cut(s) 941
PpuMI RGGWCCY 2 cut(s) 159, 924
PsiI TTATAA 2 cut(s) 1712, 1784
Psp124BI GAGCTC 2 cut(s) 530, 2216
Psp5II RGGWCCY 2 cut(s) 159, 924
Psp6I CCWGG 9 cut(s) 201, 379, 505, 583, 630, 835, 1598, 2515, 2584
PspCI CACGTG 1 cut(s) 941
PspFI CCCAGC 3 cut(s) 367, 572, 2186
PspGI CCWGG 9 cut(s) 201, 379, 505, 583, 630, 835, 1598, 2515, 2584
PspN4I GGNNCC 7 cut(s) 160, 161, 232, 741, 1075, 1492, 1760
PspPI GGNCC 6 cut(s) 159, 739, 812, 924, 1491, 1759
PspPPI RGGWCCY 2 cut(s) 159, 924
PstNI CAGNNNCTG 1 cut(s) 584
PsuI RGATCY 2 cut(s) 99, 2140
PvuII CAGCTG 1 cut(s) 1283
RsaI GTAC 9 cut(s) 30, 137, 800, 849, 872, 1075, 1833, 2260, 2401
RsaNI GTAC 9 cut(s) 29, 136, 799, 848, 871, 1074, 1832, 2259, 2400
RseI CAYNNNNRTG 2 cut(s) 1244, 1807
SacI GAGCTC 2 cut(s) 530, 2216
SaqAI TTAA 7 cut(s) 144, 399, 960, 1008, 1101, 1644, 2157
Sau3AI GATC 9 cut(s) 55, 99, 282, 451, 967, 1683, 1822, 2140, 2145
Sau96I GGNCC 6 cut(s) 159, 739, 812, 924, 1491, 1759
SchI GAGTC 6 cut(s) 119, 454, 1306, 1470, 1523, 2586
ScrFI CCNGG 9 cut(s) 203, 381, 507, 585, 632, 837, 1600, 2517, 2586
SduI GDGCHC 5 cut(s) 233, 530, 862, 2216, 2296
SexAI ACCWGGT 1 cut(s) 2515
SfaNI GCATC 1 cut(s) 168
SfcI CTRYAG 2 cut(s) 1030, 1846
SfuI TTCGAA 1 cut(s) 2005
SinI GGWCC 5 cut(s) 159, 812, 924, 1491, 1759
SmiMI CAYNNNNRTG 2 cut(s) 1244, 1807
SmlI CTYRAG 1 cut(s) 1533
SmoI CTYRAG 1 cut(s) 1533
SphI GCATGC 1 cut(s) 1743
SseBI AGGCCT 1 cut(s) 216
SsiI CCGC 9 cut(s) 26, 44, 117, 120, 419, 480, 1093, 2268, 2358
SspMI CTAG 8 cut(s) 311, 459, 546, 1503, 1658, 2127, 2277, 2340
SstI GAGCTC 2 cut(s) 530, 2216
StuI AGGCCT 1 cut(s) 216
StyD4I CCNGG 9 cut(s) 201, 379, 505, 583, 630, 835, 1598, 2515, 2584
StyI CCWWGG 1 cut(s) 211
TaiI ACGT 3 cut(s) 943, 1392, 2319
TaqI TCGA 7 cut(s) 353, 1067, 1145, 1178, 2005, 2148, 2546
TatI WGTACW 3 cut(s) 798, 847, 1831
TauI GCSGC 4 cut(s) 120, 123, 2270, 2361
TfiI GAWTC 7 cut(s) 274, 466, 977, 1180, 1194, 1421, 1577
Tru1I TTAA 7 cut(s) 144, 399, 960, 1008, 1101, 1644, 2157
Tru9I TTAA 7 cut(s) 144, 399, 960, 1008, 1101, 1644, 2157
TscAI CASTG 6 cut(s) 253, 306, 412, 988, 1471, 1844
TseFI GTSAC 4 cut(s) 82, 901, 1702, 1841
TseI GCWGC 8 cut(s) 114, 518, 618, 1088, 1189, 1283, 1499, 2071
Tsp45I GTSAC 4 cut(s) 82, 901, 1702, 1841
TspDTI ATGAA 8 cut(s) 441, 843, 1118, 1261, 1943, 1983, 2207, 2450
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 6 cut(s) 253, 306, 412, 988, 1471, 1844
Van91I CCANNNNNTGG 2 cut(s) 476, 2564
VpaK11BI GGWCC 5 cut(s) 159, 812, 924, 1491, 1759
XagI CCTNNNNNAGG 1 cut(s) 224
XbaI TCTAGA 1 cut(s) 2126
XceI RCATGY 1 cut(s) 1743
XmnI GAANNNNTTC 2 cut(s) 1576, 2001
XspI CTAG 8 cut(s) 311, 459, 546, 1503, 1658, 2127, 2277, 2340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.