Rmu_co8086728.1_g000001

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8086728.1
OG0009053
Physical Location & Seq
Forward (+)
1 .. 574
574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8086728.1_g000001.1.cds

Sequence Viewer

Length: 487 bp
ttccggcgaaccgaaccccttcaattttccggcgatggacgtgaggctaacggagggggcgatactgaagatcacgacggaagaccatgaccagcagtatagacccatcctgcaagtgcacgacatgaagcagatccactcacagggcggcgccggcgcggacaaagagcgctacaggctggccttgtccgatgggtcccacacccagcaggggatgcttgccactcagcagaacttcctgattcggcagggccacctccaggtgggctccgtcgtctgcctcaatcaattcacctgcaccaccgtccaaggccgccagtatgttctacctctctctctctctctctctctctctcttccttcctttttccaaattccatgcttttttggtcaaacttgttttgttgatgcgtgtttcgttttcgagtttgtgtttgtgattaatggaattgagaggaaaatgatgctgaagctgtgttttagttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

161

Amino Acids

18.11

Weight (kDa)

6.48

Isoelectric Point (pI)

32.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 303
Acc36I ACCTGC 1 cut(s) 303
AccB1I GGYRCC 1 cut(s) 150
AccII CGCG 1 cut(s) 159
AciI CCGC 3 cut(s) 148, 159, 314
AclWI GGATC 1 cut(s) 128
AcsI RAATTY 1 cut(s) 373
AcuI CTGAAG 1 cut(s) 87
AcyI GRCGYC 1 cut(s) 151
AfeI AGCGCT 1 cut(s) 171
AfiI CCNNNNNNNGG 4 cut(s) 143, 211, 260, 263
AgsI TTSAA 1 cut(s) 23
AjiI CACGTC 1 cut(s) 41
AjnI CCWGG 1 cut(s) 259
AluBI AGCT 1 cut(s) 473
AluI AGCT 1 cut(s) 473
Alw21I GWGCWC 1 cut(s) 121
Alw44I GTGCAC 1 cut(s) 117
AlwI GGATC 1 cut(s) 128
Aor51HI AGCGCT 1 cut(s) 171
AoxI GGCC 3 cut(s) 181, 251, 311
ApaLI GTGCAC 1 cut(s) 117
ApoI RAATTY 1 cut(s) 373
AseI ATTAAT 1 cut(s) 442
Asp700I GAANNNNTTC 1 cut(s) 18
AspLEI GCGC 3 cut(s) 153, 159, 172
AspS9I GGNCC 2 cut(s) 196, 251
AsuHPI GGTGA 1 cut(s) 284
AvaII GGWCC 1 cut(s) 196
BaeGI GKGCMC 1 cut(s) 121
BanI GGYRCC 1 cut(s) 150
BanII GRGCYC 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 121
BccI CCATC 3 cut(s) 29, 114, 186
BciT130I CCWGG 1 cut(s) 261
BfmI CTRYAG 1 cut(s) 173
BfoI RGCGCY 2 cut(s) 154, 173
BfuAI ACCTGC 1 cut(s) 303
BisI GCNGC 2 cut(s) 149, 314
BlsI GCNGC 2 cut(s) 150, 315
Bme1390I CCNGG 1 cut(s) 261
Bme18I GGWCC 1 cut(s) 196
BmgBI CACGTC 1 cut(s) 41
BmgT120I GGNCC 2 cut(s) 196, 251
BmiI GGNNCC 4 cut(s) 152, 197, 198, 269
BmrFI CCNGG 1 cut(s) 261
BmsI GCATC 3 cut(s) 205, 398, 454
BpiI GAAGAC 1 cut(s) 88
BpmI CTGGAG 1 cut(s) 243
BsaHI GRCGYC 1 cut(s) 151
BsaJI CCNNGG 1 cut(s) 308
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 211, 260, 263
Bse118I RCCGGY 1 cut(s) 153
Bse1I ACTGG 1 cut(s) 317
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 308
BseGI GGATG 2 cut(s) 106, 220
BseLI CCNNNNNNNGG 4 cut(s) 143, 211, 260, 263
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 1 cut(s) 317
BseSI GKGCMC 1 cut(s) 121
BseYI CCCAGC 1 cut(s) 205
BsgI GTGCAG 1 cut(s) 281
Bsh1236I CGCG 1 cut(s) 159
BshFI GGCC 3 cut(s) 183, 253, 313
BshNI GGYRCC 1 cut(s) 150
BsiHKAI GWGCWC 1 cut(s) 121
BsiSI CCGG 3 cut(s) 4, 30, 154
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 4 cut(s) 143, 211, 260, 263
BsmFI GGGAC 1 cut(s) 182
BsnI GGCC 3 cut(s) 183, 253, 313
Bsp1286I GDGCHC 2 cut(s) 121, 270
Bsp143I GATC 2 cut(s) 70, 133
BspACI CCGC 3 cut(s) 148, 159, 314
BspANI GGCC 3 cut(s) 183, 253, 313
BspCNI CTCAG 1 cut(s) 239
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 4 cut(s) 152, 197, 198, 269
BspMI ACCTGC 1 cut(s) 303
BspPI GGATC 1 cut(s) 128
BspT107I GGYRCC 1 cut(s) 150
BsrFI RCCGGY 1 cut(s) 153
BsrI ACTGG 1 cut(s) 317
BssAI RCCGGY 1 cut(s) 153
BssECI CCNNGG 1 cut(s) 308
BssMI GATC 2 cut(s) 70, 133
BssNI GRCGYC 1 cut(s) 151
BssT1I CCWWGG 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 1 cut(s) 361
BstACI GRCGYC 1 cut(s) 151
BstAPI GCANNNNNTGC 1 cut(s) 215
BstC8I GCNNGC 3 cut(s) 155, 181, 220
BstDEI CTNAG 1 cut(s) 226
BstF5I GGATG 2 cut(s) 106, 220
BstFNI CGCG 1 cut(s) 159
BstH2I RGCGCY 2 cut(s) 154, 173
BstHHI GCGC 3 cut(s) 153, 159, 172
BstKTI GATC 2 cut(s) 73, 136
BstMBI GATC 2 cut(s) 70, 133
BstMWI GCNNNNNNNGC 3 cut(s) 154, 176, 215
BstNI CCWGG 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 259
BstSFI CTRYAG 1 cut(s) 173
BstSLI GKGCMC 1 cut(s) 121
BstUI CGCG 1 cut(s) 159
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuRI GGCC 3 cut(s) 183, 253, 313
BtgZI GCGATG 1 cut(s) 48
BtrI CACGTC 1 cut(s) 41
BtsCI GGATG 2 cut(s) 106, 220
BveI ACCTGC 1 cut(s) 303
Cac8I GCNNGC 3 cut(s) 155, 181, 220
CfoI GCGC 3 cut(s) 153, 159, 172
Cfr10I RCCGGY 1 cut(s) 153
Cfr13I GGNCC 2 cut(s) 196, 251
CviAII CATG 3 cut(s) 87, 125, 379
CviJI RGCY 7 cut(s) 47, 179, 183, 253, 268, 313, 473
CviKI_1 RGCY 7 cut(s) 47, 179, 183, 253, 268, 313, 473
DdeI CTNAG 1 cut(s) 226
DinI GGCGCC 1 cut(s) 152
DpnI GATC 2 cut(s) 72, 135
DpnII GATC 2 cut(s) 70, 133
Eam1104I CTCTTC 1 cut(s) 361
EarI CTCTTC 1 cut(s) 361
Eco130I CCWWGG 1 cut(s) 308
Eco24I GRGCYC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 196
Eco47III AGCGCT 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 87
EcoO109I RGGNCCY 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 308
EcoT38I GRGCYC 1 cut(s) 270
EgeI GGCGCC 1 cut(s) 152
EheI GGCGCC 1 cut(s) 152
ErhI CCWWGG 1 cut(s) 308
FaeI CATG 3 cut(s) 90, 128, 382
FaiI YATR 5 cut(s) 88, 100, 126, 322, 380
FaqI GGGAC 1 cut(s) 182
FatI CATG 3 cut(s) 86, 124, 378
Fnu4HI GCNGC 2 cut(s) 149, 314
FokI GGATG 2 cut(s) 93, 227
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 2 cut(s) 149, 314
GlaI GCGC 3 cut(s) 152, 158, 171
GluI GCNGC 2 cut(s) 149, 314
GsaI CCCAGC 1 cut(s) 209
GsuI CTGGAG 1 cut(s) 243
HaeII RGCGCY 2 cut(s) 154, 173
HaeIII GGCC 3 cut(s) 183, 253, 313
HapII CCGG 3 cut(s) 4, 30, 154
HhaI GCGC 3 cut(s) 153, 159, 172
Hin1I GRCGYC 1 cut(s) 151
Hin1II CATG 3 cut(s) 90, 128, 382
Hin6I GCGC 3 cut(s) 151, 157, 170
HinP1I GCGC 3 cut(s) 151, 157, 170
HinfI GANTC 1 cut(s) 242
HpaII CCGG 3 cut(s) 4, 30, 154
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 1 cut(s) 119
Hpy188I TCNGA 1 cut(s) 191
Hpy188III TCNNGA 2 cut(s) 74, 239
Hpy8I GTNNAC 1 cut(s) 119
Hpy99I CGWCG 2 cut(s) 80, 276
HpyAV CCTTC 2 cut(s) 29, 370
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 1 cut(s) 40
HpyCH4V TGCA 3 cut(s) 113, 119, 298
HpyF10VI GCNNNNNNNGC 3 cut(s) 154, 176, 215
HpyF3I CTNAG 1 cut(s) 226
HpySE526I ACGT 1 cut(s) 40
Hsp92I GRCGYC 1 cut(s) 151
Hsp92II CATG 3 cut(s) 90, 128, 382
HspAI GCGC 3 cut(s) 151, 157, 170
KasI GGCGCC 1 cut(s) 150
KflI GGGWCCC 1 cut(s) 196
KroI GCCGGC 1 cut(s) 153
KroNI GCCGGC 1 cut(s) 155
Kzo9I GATC 2 cut(s) 70, 133
LmnI GCTCC 1 cut(s) 273
LweI GCATC 3 cut(s) 205, 398, 454
MaeII ACGT 1 cut(s) 40
MalI GATC 2 cut(s) 72, 135
MboI GATC 2 cut(s) 70, 133
MboII GAAGA 3 cut(s) 80, 93, 348
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 2 cut(s) 121, 270
MluCI AATT 4 cut(s) 23, 288, 373, 448
Mly113I GGCGCC 1 cut(s) 151
MnlI CCTC 6 cut(s) 37, 47, 267, 291, 340, 448
MreI CGCCGGCG 1 cut(s) 153
MroNI GCCGGC 1 cut(s) 153
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 2 cut(s) 442, 485
MspI CCGG 3 cut(s) 4, 30, 154
MspR9I CCNGG 1 cut(s) 261
MvaI CCWGG 1 cut(s) 261
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 3 cut(s) 154, 176, 215
NaeI GCCGGC 1 cut(s) 155
NarI GGCGCC 1 cut(s) 151
NdeII GATC 2 cut(s) 70, 133
NgoMIV GCCGGC 1 cut(s) 153
NlaIII CATG 3 cut(s) 90, 128, 382
NlaIV GGNNCC 4 cut(s) 152, 197, 198, 269
PaqCI CACCTGC 1 cut(s) 303
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PdiI GCCGGC 1 cut(s) 155
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 1 cut(s) 242
PkrI GCNGC 2 cut(s) 150, 315
PluTI GGCGCC 1 cut(s) 154
PpuMI RGGWCCY 1 cut(s) 196
PshBI ATTAAT 1 cut(s) 442
Psp5II RGGWCCY 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 259
PspFI CCCAGC 1 cut(s) 205
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 4 cut(s) 152, 197, 198, 269
PspPI GGNCC 2 cut(s) 196, 251
PspPPI RGGWCCY 1 cut(s) 196
PsuI RGATCY 1 cut(s) 133
SaqAI TTAA 2 cut(s) 442, 485
SatI GCNGC 2 cut(s) 149, 314
Sau3AI GATC 2 cut(s) 70, 133
Sau96I GGNCC 2 cut(s) 196, 251
ScrFI CCNGG 1 cut(s) 261
SduI GDGCHC 2 cut(s) 121, 270
SetI ASST 6 cut(s) 43, 259, 265, 297, 332, 475
SfaNI GCATC 3 cut(s) 205, 398, 454
SfcI CTRYAG 1 cut(s) 173
SfoI GGCGCC 1 cut(s) 152
SgrAI CRCCGGYG 1 cut(s) 153
SinI GGWCC 1 cut(s) 196
Sse9I AATT 4 cut(s) 23, 288, 373, 448
SsiI CCGC 3 cut(s) 148, 159, 314
SspDI GGCGCC 1 cut(s) 150
StyD4I CCNGG 1 cut(s) 259
StyI CCWWGG 1 cut(s) 308
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 1 cut(s) 43
TaqI TCGA 1 cut(s) 424
TasI AATT 4 cut(s) 23, 288, 373, 448
TauI GCSGC 2 cut(s) 151, 316
TfiI GAWTC 1 cut(s) 242
Tru1I TTAA 2 cut(s) 442, 485
Tru9I TTAA 2 cut(s) 442, 485
TspDTI ATGAA 1 cut(s) 141
TspGWI ACGGA 3 cut(s) 66, 93, 260
VneI GTGCAC 1 cut(s) 117
VpaK11BI GGWCC 1 cut(s) 196
VspI ATTAAT 1 cut(s) 442
XapI RAATTY 1 cut(s) 373
XmnI GAANNNNTTC 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.