Rh7BG268900

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0009053
Physical Location & Seq
Reverse (-)
24678243 .. 24683683
5441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG268900.1

Sequence Viewer

Length: 2643 bp
ATGGACGTGAGGCTAACGGAGGGGGCGATACTGAAGATCACGACGGAGCCCCATGACGAGCAGTATAGGCCCATCCTGCAAGTGCTCGACCTGAAGCAGATCCACGCACGCGGCGGCGGCTCGAACACCGAGCGCTACAGGCTGGCCTTGTCCGATGGGTCCCACACCCAGCAGGGGATGCTTGCCACTCAGCAGAACTTCCTGGTCCAGCAGGGCCACCTCCAGAAGGGCTCCGTAGTCTGCCTCAACCAATACACCTGCACCGCCGTCCAAGGCCGCCAGATCATCATTATAGTCTCATTGGATGTGCTAGTTAATACATGTGAAATCATTGGACAGCCTGCCACATTTTCGAATGCACAGTCCCCAGCTACAATACCTGGAAATGCCCAATCTCTTAACAGCAGTGCTCGTGTGGCGGGTCATGAAACTGTGACTGAGGCAACTCCCCAACAACCTAGATTGAATCAATCATATGGTGGTTCTTATTCTGGTGGTGCTGACCCAGGAAGATATGCTACAATGAGTACAGTTCCCAATCACCCTAGACCAGAGACTGGTTCTGGTTTTGCTGGGTTAGCACCAGTAAGTGGGTCATATGCTGGTCAGAACACAGGTTTACGCAGCCCCATATCCCAGGTTCCACCTCCACTTCAAAATTCTTATGCTCACCCACCTCAGCCGACTTATCAACAGTCACACTCAAATTCTTATGCCCGCCCGCCTCAGGCAACTTATCAACAGCCACCTCCAAGTTCTTATGCACGCCCACCTCAGCCAACTCATCAACAGCCACCTCCATTATATAGTAATAGAGGACCAGTTGCCAAAAATGAAGCTCCTGGTATAATAGTACAAATTTGTGCTCTGAATCGATACCTAAATAGGTGGACAATTAAGGCTAGAGTGGCGGCTAAATCAGAACTCAGGTCCTACACCAATGCACGTGGTGAGGGGAAAGTATTTAATTTTGATCTTATTGATTCTTCTGGCGAAATTCGTGTAACCTGCTTTAATCCAGTGGCAGAAGAGTTCTACAATTTGATTGAAGTTGGTAAGCTTTACATGATCTCAAAGGGTAGTGTGAAGGCAGCTGTAAGGAAATTCAACCACCTCAACAACGAGTTTGAAATTCAATTGGACACCACTTCAACAATACAGTTATGTTTTGAGGATGATGGCTCAATTCCACAGCAGCGGTTCAATTTTCGGCCAATAAGTGATGTTGAAGGCTTGGGGAACGACATTATTGTGGATATAATAGGTGTGGTAACATTCATCAGTCCTGCCGCTTCAATAAGGACGAAAAACGGTGAGACACAAAAGAGAACTCTCCAGTTGAAGGATATGTCTTGTCGTAGCGTTGAAGTAACTCTCTGGGGCAATTTGTGCAATGTAGAAGGGCAAAAACTTCAAAGTATGTGTGATTCTGGGTTATTTCCAGTGCTAGCTGTTAAAGCTGGTAGAGTCAATGACTTCAATGGCAAGGGGGTGGGGACCATGGCTACTAGTCAGCTCTTTATAGAGCCCAATAACTCTCAAGCTGATGAGGTGAGGGAATGGTTTGACAGGGAAGGAAGGAATTCCCCATGTGTCTCTATTTCCAGGGGAACAGCAAGTGTGGGAAGAAATGATATCCGAAAGATTATATCCCAGATTAAAGATGAGAAGCTAGGGACTTCTGAGAAGCCAGATTGGATCACAGTCAGTGCTACTGTCATATTTATAAAGAGTGATAACTACTTCTATGCAGCATGCCCACTCAAGGTAGGGGAGCGGCAATGCAACAAAAAGGTTATAAATGATGGAGATGGGACATGGCGGTGTGAAAGATGTGAGCAGTCCATAGAACAGTGTGACTACAGATATATACTCAACTTACAGATACAAGACCATACTGGCATAGCATGGGTAACTGCATTTCAGGAGGCTGGTGAAGAAATGATGGGTATAACTGCAAAAGATTTTCATTATTTGAAACATGAAAAGCAGGATGAAGATAAAGTTGCAGAAATCATTCGAAGGGTCTTGTTTACTGAATTCGTTTTCAAGTTGAAAATAAAGGAGGAGACTTTTAGTGATGAGCAGCGTGTGAAATCAACAATTGTCAAAGCAGATCAAGTTAGTTTCTCTTCAAAATCTAGATTTCATTTAGATCTGATGGATAAACTTAAAAATGGGGAAACTGCAATACCTTCTGCTGGGATGAACAATACTGAAGTGGGGAGCTCTGCAATTAGACAACATACACCAGCTGTGAATGCTAGTTACAGTACCAATGCTGCCAATGCTGGTAGAGAGTTTGGAACACCAGCAAATCAAGTACATGTTGGAAGCCAGTATATTAGTCCTAGGGTTCCTTCAACCGGCTCCGCTGGTTTGTATCCTACCTGTAACATCTGTCGAGGTACAAGCCATAATTCTTTGAACTGCCCAAATGTTATGAATGGCCCAGGACAGTCATCAGGAGGGGGTTACACCAATAGTAGGCCAGTTGCCAGTAATTCTTCACCATGCTATAAATGTCACCAACCTGGGCACTGGGCGAAAGACTGTCCTAGCTCGAACCATATTCCAGCTTATGGAAGTAGTGGAGTCCAGCCAGGCAGATATGGAGTTTCAAATCAAAGGGTCAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon zf-CCHC

Protein Analysis

880

Amino Acids

96.45

Weight (kDa)

8.42

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 5 - 105 1.8e-26 Replication factor-A protein 1, N-terminal domain
tRNA_anti-codon PF01336 297 - 380 5.6e-10 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 406 - 508 7e-24 Replication protein A OB domain
Rep_fac-A_C PF08646 566 - 715 3e-50 Replication factor-A C terminal domain
zf-CCHC PF00098 838 - 854 2.8e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1727, 1799
AarI CACCTGC 1 cut(s) 266
Acc36I ACCTGC 2 cut(s) 266, 1016
AccB7I CCANNNNNTGG 3 cut(s) 557, 590, 2582
AccBSI CCGCTC 1 cut(s) 1777
AccII CGCG 1 cut(s) 111
AclWI GGATC 2 cut(s) 94, 1706
AcoI YGGCCR 1 cut(s) 1211
AcsI RAATTY 8 cut(s) 658, 706, 858, 996, 1103, 1131, 1582, 2039
AcuI CTGAAG 3 cut(s) 53, 113, 2238
AcvI CACGTG 1 cut(s) 947
AdeI CACNNNGTG 1 cut(s) 950
AfaI GTAC 5 cut(s) 529, 855, 2275, 2325, 2410
AfeI AGCGCT 1 cut(s) 134
AflIII ACRYGT 2 cut(s) 320, 2326
AhlI ACTAGT 1 cut(s) 1508
AjiI CACGTC 1 cut(s) 7
AjnI CCWGG 9 cut(s) 201, 379, 505, 636, 841, 1604, 2452, 2533, 2602
Alw21I GWGCWC 4 cut(s) 87, 412, 868, 2231
Alw26I GTCTC 5 cut(s) 301, 548, 1310, 1600, 2063
AlwI GGATC 2 cut(s) 94, 1706
AlwNI CAGNNNCTG 1 cut(s) 557
Aor51HI AGCGCT 1 cut(s) 134
AoxI GGCC 7 cut(s) 68, 144, 214, 274, 1211, 2449, 2489
ApeKI GCWGC 6 cut(s) 624, 1091, 1195, 1751, 2086, 2282
ApoI RAATTY 8 cut(s) 658, 706, 858, 996, 1103, 1131, 1582, 2039
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
Asp700I GAANNNNTTC 2 cut(s) 1582, 2016
AspA2I CCTAGG 1 cut(s) 2351
AspLEI GCGC 1 cut(s) 135
AspS9I GGNCC 8 cut(s) 69, 159, 205, 214, 818, 930, 1497, 2450
AsuHPI GGTGA 8 cut(s) 533, 662, 962, 1325, 1564, 1946, 2502, 2519
AsuII TTCGAA 2 cut(s) 353, 2020
AsuNHI GCTAGC 1 cut(s) 1447
AvaII GGWCC 5 cut(s) 159, 205, 818, 930, 1497
AvrII CCTAGG 1 cut(s) 2351
AxyI CCTNAGG 1 cut(s) 726
BaeGI GKGCMC 1 cut(s) 2541
BanII GRGCYC 4 cut(s) 51, 233, 1530, 2231
BarI GAAGNNNNNNTAC 2 cut(s) 502, 534
BauI CACGAG 1 cut(s) 411
BbrPI CACGTG 1 cut(s) 947
Bbv12I GWGCWC 4 cut(s) 87, 412, 868, 2231
BbvCI CCTCAGC 2 cut(s) 678, 774
BbvI GCAGC 6 cut(s) 636, 1103, 1207, 1763, 2098, 2269
BccI CCATC 7 cut(s) 80, 149, 1172, 1799, 1805, 1939, 2155
BceAI ACGGC 1 cut(s) 251
BciT130I CCWGG 9 cut(s) 203, 381, 507, 638, 843, 1606, 2454, 2535, 2604
BciVI GTATCC 1 cut(s) 2394
BcoDI GTCTC 5 cut(s) 301, 548, 1310, 1600, 2063
BcuI ACTAGT 1 cut(s) 1508
BfmI CTRYAG 2 cut(s) 136, 1861
BfoI RGCGCY 1 cut(s) 136
BfuAI ACCTGC 2 cut(s) 266, 1016
BfuI GTATCC 1 cut(s) 2394
BglII AGATCT 1 cut(s) 2155
BlnI CCTAGG 1 cut(s) 2351
Bme1390I CCNGG 9 cut(s) 203, 381, 507, 638, 843, 1606, 2454, 2535, 2604
Bme18I GGWCC 5 cut(s) 159, 205, 818, 930, 1497
BmgBI CACGTC 1 cut(s) 7
BmgT120I GGNCC 8 cut(s) 69, 159, 205, 214, 818, 930, 1497, 2450
BmiI GGNNCC 8 cut(s) 48, 160, 161, 232, 642, 1498, 2358, 2371
BmrFI CCNGG 9 cut(s) 203, 381, 507, 638, 843, 1606, 2454, 2535, 2604
BmrI ACTGGG 1 cut(s) 2551
BmsI GCATC 1 cut(s) 168
BmtI GCTAGC 1 cut(s) 1451
BmuI ACTGGG 1 cut(s) 2551
BpmI CTGGAG 2 cut(s) 206, 1319
Bpu10I CCTNAGC 2 cut(s) 678, 774
Bpu14I TTCGAA 2 cut(s) 353, 2020
BpuEI CTTGAG 2 cut(s) 1524, 1748
Bsa29I ATCGAT 1 cut(s) 874
BsaAI YACGTR 1 cut(s) 947
BsaJI CCNNGG 8 cut(s) 271, 505, 636, 1500, 1605, 2351, 2452, 2534
Bse118I RCCGGY 1 cut(s) 2366
Bse21I CCTNAGG 1 cut(s) 726
Bse3DI GCAATG 2 cut(s) 1399, 1787
BseBI CCWGG 9 cut(s) 203, 381, 507, 638, 843, 1606, 2454, 2535, 2604
BseCI ATCGAT 1 cut(s) 874
BseDI CCNNGG 8 cut(s) 271, 505, 636, 1500, 1605, 2351, 2452, 2534
BseGI GGATG 6 cut(s) 72, 183, 310, 1180, 1999, 2211
BseMI GCAATG 2 cut(s) 1399, 1787
BseMII CTCAG 7 cut(s) 203, 429, 692, 740, 788, 940, 1674
BseRI GAGGAG 1 cut(s) 2081
BseSI GKGCMC 1 cut(s) 2541
BseXI GCAGC 6 cut(s) 636, 1103, 1207, 1763, 2098, 2269
BseYI CCCAGC 4 cut(s) 168, 367, 572, 2201
BsgI GTGCAG 1 cut(s) 244
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 7 cut(s) 70, 146, 216, 276, 1213, 2451, 2491
BshVI ATCGAT 1 cut(s) 874
BsiHKAI GWGCWC 4 cut(s) 87, 412, 868, 2231
BsiSI CCGG 1 cut(s) 2367
BslFI GGGAC 5 cut(s) 145, 349, 1510, 1690, 1828
BsmAI GTCTC 5 cut(s) 301, 548, 1310, 1600, 2063
BsmFI GGGAC 5 cut(s) 145, 349, 1510, 1690, 1828
BsmI GAATGC 2 cut(s) 361, 2266
BsnI GGCC 7 cut(s) 70, 146, 216, 276, 1213, 2451, 2491
Bsp119I TTCGAA 2 cut(s) 353, 2020
Bsp1286I GDGCHC 8 cut(s) 51, 87, 233, 412, 868, 1530, 2231, 2541
Bsp143I GATC 8 cut(s) 36, 99, 282, 973, 1068, 1698, 2116, 2155
Bsp19I CCATGG 1 cut(s) 1500
BspANI GGCC 7 cut(s) 70, 146, 216, 276, 1213, 2451, 2491
BspCNI CTCAG 7 cut(s) 202, 430, 691, 739, 787, 939, 1675
BspDI ATCGAT 1 cut(s) 874
BspFNI CGCG 1 cut(s) 111
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 8 cut(s) 48, 160, 161, 232, 642, 1498, 2358, 2371
BspMI ACCTGC 2 cut(s) 266, 1016
BspOI GCTAGC 1 cut(s) 1451
BspPI GGATC 2 cut(s) 94, 1706
BspT104I TTCGAA 2 cut(s) 353, 2020
BsrBI CCGCTC 1 cut(s) 1777
BsrDI GCAATG 2 cut(s) 1399, 1787
BsrFI RCCGGY 1 cut(s) 2366
BssAI RCCGGY 1 cut(s) 2366
BssECI CCNNGG 8 cut(s) 271, 505, 636, 1500, 1605, 2351, 2452, 2534
BssMI GATC 8 cut(s) 36, 99, 282, 973, 1068, 1698, 2116, 2155
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 3 cut(s) 271, 1500, 2351
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 9 cut(s) 203, 381, 507, 638, 843, 1606, 2454, 2535, 2604
Bst6I CTCTTC 2 cut(s) 1023, 2137
BstAPI GCANNNNNTGC 2 cut(s) 178, 1389
BstBAI YACGTR 1 cut(s) 947
BstBI TTCGAA 2 cut(s) 353, 2020
BstC8I GCNNGC 9 cut(s) 109, 144, 183, 342, 718, 722, 766, 1449, 1756
BstDEI CTNAG 7 cut(s) 189, 438, 678, 726, 774, 926, 1683
BstDSI CCRYGG 1 cut(s) 1500
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 6 cut(s) 72, 183, 310, 1180, 1999, 2211
BstFNI CGCG 1 cut(s) 111
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 1 cut(s) 135
BstKTI GATC 8 cut(s) 39, 102, 285, 976, 1071, 1701, 2119, 2158
BstMAI GTCTC 5 cut(s) 301, 548, 1310, 1600, 2063
BstMBI GATC 8 cut(s) 36, 99, 282, 973, 1068, 1698, 2116, 2155
BstNI CCWGG 9 cut(s) 203, 381, 507, 638, 843, 1606, 2454, 2535, 2604
BstNSI RCATGY 3 cut(s) 324, 1758, 2330
BstSCI CCNGG 9 cut(s) 201, 379, 505, 636, 841, 1604, 2452, 2533, 2602
BstSFI CTRYAG 2 cut(s) 136, 1861
BstSLI GKGCMC 1 cut(s) 2541
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 6 cut(s) 636, 1103, 1207, 1763, 2098, 2269
BstX2I RGATCY 2 cut(s) 99, 2155
BstYI RGATCY 2 cut(s) 99, 2155
Bsu15I ATCGAT 1 cut(s) 874
Bsu36I CCTNAGG 1 cut(s) 726
BsuI GTATCC 1 cut(s) 2394
BsuRI GGCC 7 cut(s) 70, 146, 216, 276, 1213, 2451, 2491
BsuTUI ATCGAT 1 cut(s) 874
BtgI CCRYGG 1 cut(s) 1500
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 6 cut(s) 72, 183, 310, 1180, 1999, 2211
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 6 cut(s) 412, 1026, 1449, 1714, 1859, 2539
BveI ACCTGC 2 cut(s) 266, 1016
Cac8I GCNNGC 9 cut(s) 109, 144, 183, 342, 718, 722, 766, 1449, 1756
CaiI CAGNNNCTG 1 cut(s) 557
CciI TCATGA 1 cut(s) 424
CfoI GCGC 1 cut(s) 135
Cfr10I RCCGGY 1 cut(s) 2366
Cfr13I GGNCC 8 cut(s) 69, 159, 205, 214, 818, 930, 1497, 2450
ClaI ATCGAT 1 cut(s) 874
Csp6I GTAC 5 cut(s) 528, 854, 2274, 2324, 2409
CspCI CAANNNNNGTGG 2 cut(s) 928, 963
CviQI GTAC 5 cut(s) 528, 854, 2274, 2324, 2409
DdeI CTNAG 7 cut(s) 189, 438, 678, 726, 774, 926, 1683
DpnI GATC 8 cut(s) 38, 101, 284, 975, 1070, 1700, 2118, 2157
DpnII GATC 8 cut(s) 36, 99, 282, 973, 1068, 1698, 2116, 2155
DraIII CACNNNGTG 1 cut(s) 950
EaeI YGGCCR 1 cut(s) 1211
Eam1104I CTCTTC 2 cut(s) 1023, 2137
EarI CTCTTC 2 cut(s) 1023, 2137
Ecl136II GAGCTC 1 cut(s) 2229
Eco130I CCWWGG 3 cut(s) 271, 1500, 2351
Eco24I GRGCYC 4 cut(s) 51, 233, 1530, 2231
Eco32I GATATC 1 cut(s) 1636
Eco47I GGWCC 5 cut(s) 159, 205, 818, 930, 1497
Eco47III AGCGCT 1 cut(s) 134
Eco53kI GAGCTC 1 cut(s) 2229
Eco57I CTGAAG 3 cut(s) 53, 113, 2238
Eco72I CACGTG 1 cut(s) 947
Eco81I CCTNAGG 1 cut(s) 726
EcoICRI GAGCTC 1 cut(s) 2229
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoO109I RGGNCCY 2 cut(s) 159, 930
EcoRI GAATTC 2 cut(s) 1582, 2039
EcoRII CCWGG 9 cut(s) 201, 379, 505, 636, 841, 1604, 2452, 2533, 2602
EcoRV GATATC 1 cut(s) 1636
EcoT14I CCWWGG 3 cut(s) 271, 1500, 2351
EcoT38I GRGCYC 4 cut(s) 51, 233, 1530, 2231
ErhI CCWWGG 3 cut(s) 271, 1500, 2351
FaqI GGGAC 5 cut(s) 145, 349, 1510, 1690, 1828
FauI CCCGC 3 cut(s) 412, 725, 729
FauNDI CATATG 2 cut(s) 475, 598
FokI GGATG 6 cut(s) 59, 190, 317, 1187, 2006, 2218
FriOI GRGCYC 4 cut(s) 51, 233, 1530, 2231
GlaI GCGC 1 cut(s) 134
GsaI CCCAGC 4 cut(s) 172, 371, 576, 2205
GsuI CTGGAG 2 cut(s) 206, 1319
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 7 cut(s) 70, 146, 216, 276, 1213, 2451, 2491
HapII CCGG 1 cut(s) 2367
HhaI GCGC 1 cut(s) 135
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HindIII AAGCTT 1 cut(s) 1058
HinfI GANTC 6 cut(s) 466, 871, 983, 1427, 1467, 2595
HpaII CCGG 1 cut(s) 2367
HphI GGTGA 8 cut(s) 533, 662, 962, 1325, 1564, 1946, 2502, 2519
Hpy166II GTNNAC 3 cut(s) 620, 891, 2034
Hpy188I TCNGA 7 cut(s) 154, 609, 870, 922, 1640, 1684, 2160
Hpy188III TCNNGA 6 cut(s) 40, 223, 425, 1925, 2142, 2466
Hpy8I GTNNAC 3 cut(s) 620, 891, 2034
Hpy99I CGWCG 1 cut(s) 46
HpyCH4IV ACGT 2 cut(s) 6, 946
HpyF3I CTNAG 7 cut(s) 189, 438, 678, 726, 774, 926, 1683
HpySE526I ACGT 2 cut(s) 6, 946
HspAI GCGC 1 cut(s) 133
KflI GGGWCCC 1 cut(s) 159
Kzo9I GATC 8 cut(s) 36, 99, 282, 973, 1068, 1698, 2116, 2155
LmnI GCTCC 6 cut(s) 46, 236, 844, 1774, 2226, 2375
Lsp1109I GCAGC 6 cut(s) 636, 1103, 1207, 1763, 2098, 2269
LweI GCATC 1 cut(s) 168
MaeII ACGT 2 cut(s) 6, 946
MalI GATC 8 cut(s) 38, 101, 284, 975, 1070, 1700, 2118, 2157
MbiI CCGCTC 1 cut(s) 1777
MboI GATC 8 cut(s) 36, 99, 282, 973, 1068, 1698, 2116, 2155
MboII GAAGA 9 cut(s) 46, 522, 978, 1040, 1638, 1949, 2009, 2124, 2499
MfeI CAATTG 2 cut(s) 1136, 2103
MflI RGATCY 2 cut(s) 99, 2155
MhlI GDGCHC 8 cut(s) 51, 87, 233, 412, 868, 1530, 2231, 2541
MlyI GAGTC 2 cut(s) 1476, 2604
MmeI TCCRAC 1 cut(s) 2311
MroXI GAANNNNTTC 2 cut(s) 1582, 2016
MseI TTAA 8 cut(s) 315, 399, 897, 966, 1014, 1455, 1659, 2172
MslI CAYNNNNRTG 2 cut(s) 1250, 1822
MspA1I CMGCKG 4 cut(s) 1094, 1198, 2255, 2375
MspI CCGG 1 cut(s) 2367
MspR9I CCNGG 9 cut(s) 203, 381, 507, 638, 843, 1606, 2454, 2535, 2604
MunI CAATTG 2 cut(s) 1136, 2103
Mva1269I GAATGC 2 cut(s) 361, 2266
MvaI CCWGG 9 cut(s) 203, 381, 507, 638, 843, 1606, 2454, 2535, 2604
MvnI CGCG 1 cut(s) 111
NcoI CCATGG 1 cut(s) 1500
NdeI CATATG 2 cut(s) 475, 598
NdeII GATC 8 cut(s) 36, 99, 282, 973, 1068, 1698, 2116, 2155
NheI GCTAGC 1 cut(s) 1447
NlaIV GGNNCC 8 cut(s) 48, 160, 161, 232, 642, 1498, 2358, 2371
NmuCI GTSAC 4 cut(s) 433, 696, 1856, 2525
NspI RCATGY 3 cut(s) 324, 1758, 2330
NspV TTCGAA 2 cut(s) 353, 2020
PaeI GCATGC 1 cut(s) 1758
PagI TCATGA 1 cut(s) 424
PaqCI CACCTGC 1 cut(s) 266
PciI ACATGT 2 cut(s) 320, 2326
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 2 cut(s) 361, 2266
PdmI GAANNNNTTC 2 cut(s) 1582, 2016
PfeI GAWTC 4 cut(s) 466, 871, 983, 1427
PflMI CCANNNNNTGG 3 cut(s) 557, 590, 2582
PleI GAGTC 2 cut(s) 1475, 2603
PmaCI CACGTG 1 cut(s) 947
PmlI CACGTG 1 cut(s) 947
PpsI GAGTC 2 cut(s) 1475, 2603
Ppu21I YACGTR 1 cut(s) 947
PpuMI RGGWCCY 2 cut(s) 159, 930
PscI ACATGT 2 cut(s) 320, 2326
PsiI TTATAA 2 cut(s) 1727, 1799
Psp124BI GAGCTC 1 cut(s) 2231
Psp5II RGGWCCY 2 cut(s) 159, 930
Psp6I CCWGG 9 cut(s) 201, 379, 505, 636, 841, 1604, 2452, 2533, 2602
PspCI CACGTG 1 cut(s) 947
PspFI CCCAGC 4 cut(s) 168, 367, 572, 2201
PspGI CCWGG 9 cut(s) 201, 379, 505, 636, 841, 1604, 2452, 2533, 2602
PspN4I GGNNCC 8 cut(s) 48, 160, 161, 232, 642, 1498, 2358, 2371
PspPI GGNCC 8 cut(s) 69, 159, 205, 214, 818, 930, 1497, 2450
PspPPI RGGWCCY 2 cut(s) 159, 930
PstNI CAGNNNCTG 1 cut(s) 557
PsuI RGATCY 2 cut(s) 99, 2155
PvuII CAGCTG 2 cut(s) 1094, 2255
RsaI GTAC 5 cut(s) 529, 855, 2275, 2325, 2410
RsaNI GTAC 5 cut(s) 528, 854, 2274, 2324, 2409
RseI CAYNNNNRTG 2 cut(s) 1250, 1822
SacI GAGCTC 1 cut(s) 2231
SaqAI TTAA 8 cut(s) 315, 399, 897, 966, 1014, 1455, 1659, 2172
Sau3AI GATC 8 cut(s) 36, 99, 282, 973, 1068, 1698, 2116, 2155
Sau96I GGNCC 8 cut(s) 69, 159, 205, 214, 818, 930, 1497, 2450
SchI GAGTC 2 cut(s) 1476, 2604
ScrFI CCNGG 9 cut(s) 203, 381, 507, 638, 843, 1606, 2454, 2535, 2604
SduI GDGCHC 8 cut(s) 51, 87, 233, 412, 868, 1530, 2231, 2541
SfaNI GCATC 1 cut(s) 168
SfcI CTRYAG 2 cut(s) 136, 1861
SfuI TTCGAA 2 cut(s) 353, 2020
SinI GGWCC 5 cut(s) 159, 205, 818, 930, 1497
SmiMI CAYNNNNRTG 2 cut(s) 1250, 1822
SmlI CTYRAG 2 cut(s) 1539, 1763
SmoI CTYRAG 2 cut(s) 1539, 1763
SpeI ACTAGT 1 cut(s) 1508
SphI GCATGC 1 cut(s) 1758
SstI GAGCTC 1 cut(s) 2231
StyD4I CCNGG 9 cut(s) 201, 379, 505, 636, 841, 1604, 2452, 2533, 2602
StyI CCWWGG 3 cut(s) 271, 1500, 2351
TaiI ACGT 2 cut(s) 9, 949
TaqI TCGA 7 cut(s) 87, 122, 353, 874, 2020, 2404, 2564
TatI WGTACW 3 cut(s) 527, 853, 2323
TauI GCSGC 7 cut(s) 114, 117, 120, 279, 914, 1292, 1780
TfiI GAWTC 4 cut(s) 466, 871, 983, 1427
Tru1I TTAA 8 cut(s) 315, 399, 897, 966, 1014, 1455, 1659, 2172
Tru9I TTAA 8 cut(s) 315, 399, 897, 966, 1014, 1455, 1659, 2172
TscAI CASTG 6 cut(s) 412, 1026, 1449, 1714, 1859, 2546
TseFI GTSAC 4 cut(s) 433, 696, 1856, 2525
TseI GCWGC 6 cut(s) 624, 1091, 1195, 1751, 2086, 2282
Tsp45I GTSAC 4 cut(s) 433, 696, 1856, 2525
TspDTI ATGAA 9 cut(s) 441, 849, 1267, 1958, 1998, 2010, 2138, 2222, 2459
TspGWI ACGGA 3 cut(s) 32, 59, 223
TspRI CASTG 6 cut(s) 412, 1026, 1449, 1714, 1859, 2546
Van91I CCANNNNNTGG 3 cut(s) 557, 590, 2582
VpaK11BI GGWCC 5 cut(s) 159, 205, 818, 930, 1497
XagI CCTNNNNNAGG 1 cut(s) 224
XapI RAATTY 8 cut(s) 658, 706, 858, 996, 1103, 1131, 1582, 2039
XbaI TCTAGA 1 cut(s) 2141
XceI RCATGY 3 cut(s) 324, 1758, 2330
XmaJI CCTAGG 1 cut(s) 2351
XmnI GAANNNNTTC 2 cut(s) 1582, 2016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.